7 results on '"Alita Moura de Lima"'
Search Results
2. Antifungal activity of extracts and phenolic compounds from Deguelia duckeana
- Author
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Lorena M. Cursino-Hron, Cecilia Veronica Nunez, João Vicente Braga de Souza, Jane V.N. Marinho, André C. de Oliveira, Alita Moura de Lima, and Nerilson M. Lima
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Phytochemistry ,Lignan ,Cytotoxicity ,Flavonoid ,lcsh:RS1-441 ,3,4,7 Trimethoxyflavone ,5,4 Dihydroxy Isolonchocarpine ,01 natural sciences ,Chalcone ,Chalcone Derivative ,General Pharmacology, Toxicology and Pharmaceutics ,Candida albicans ,Timbó ,chemistry.chemical_classification ,biology ,Traditional medicine ,3,5,4 Trimethoxy Stilbene ,Corpus albicans ,Amazonian plant ,3,5,4 Trimethoxy 4 Prenylstilbene ,3,4,7 Trimethoxy Flavonol ,Phytochemical ,Flavone Derivative ,Minimum Inhibitory Concentration ,Anti-fungal Activity ,4 Hydroxylonchocarpine ,Cryptococcus Neoformans ,Carbon Nuclear Magnetic Resonance ,3,4 Methylenedioxy 7 Methoxyflavone ,Plant Extract ,Deguelia Duckeana ,lcsh:Pharmacy and materia medica ,Minimum inhibitory concentration ,Unclassified Drug ,medicine ,4 Hydroxyderricidine ,Cryptococcus gattii ,Cryptococcus neoformans ,Liquid Chromatography-mass Spectrometry ,Derricidine ,010405 organic chemistry ,010401 analytical chemistry ,biology.organism_classification ,medicine.disease ,Nonhuman ,0104 chemical sciences ,Cryptococcus Gattii ,chemistry ,Stilbene ,Cryptococcosis ,Deguelia Duckeana Extract ,Proton Nuclear Magnetic Resonance ,Plant Medicinal Product ,Yangambin ,4 Hydroxyderricine - Abstract
Candida spp. is associated with almost 80% of all nosocomial fungal infections and is considered a major cause of blood stream infections. In humans, Cryptococcosis is a disease of the lungs caused by the fungi Cryptococcus gattii and Cryptococcus neoformans. It can be potentially fatal, especially in immune-compromised patients. In a search for antifungal drugs, Deguelia duckeana extracts were assayed against these two fungi and also against Candida albicans, which causes candidiasis. Hexane branches and CH2Cl2 root extracts as well as the substances 4-hydroxylonchocarpine, 3,5,4′-trimethoxy-4-prenylstilbene and 3′,4′-methylenedioxy-7-methoxyflavone were assayed to determine the minimal inhibitory concentration. Phytochemical study of CH2Cl2 root and hexane branch extracts from D. duckeana A.M.G. Azevedo, Fabaceae, resulted in the isolation and characterization of nine phenolic compounds: 4-hydroxyderricine, 4-hydroxylonchocarpine, 3′,4′,7-trimethoxy-flavonol, 5,4′-dihydroxy-isolonchocarpine, 4-hydroxyderricidine, derricidine, 3,5,4′-trimethoxy-stilbene, 3′,4′,7-trimethoxyflavone and yangambin. The only active extract was a CH2Cl2 root showing minimal inhibitory concentration 800 μg/ml against C. gattii, and the investigation of compounds obtained from this extract showed that 4-hydroxylonchocarpine was active against all three fungi (C. neoformans, C. gattii and C. albicans). These results suggest that D. duckeana extracts have potential therapeutic value for the treatment of pathogenic fungi. Keywords: Amazonian plant, Chalcone, Flavonoid, Lignan, Stilbene, Timbó more...
- Published
- 2018
Catalog
3. Bioprospecting of Amazon soil fungi with the potential for pigment production
- Author
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Alita Moura de Lima, Maria da Paz Lima, João Vicente Braga de Souza, Jessyca dos Reis Celestino, Loretta Ennes de Carvalho, and Mauricio Morishi Ogusku
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Bioprospecting ,Aspergillus calidoustus ,biology ,Serial dilution ,Amazon rainforest ,Rhamnose ,Penicillium purpurogenum ,food and beverages ,Bioengineering ,biology.organism_classification ,Applied Microbiology and Biotechnology ,Biochemistry ,Pigment ,chemistry.chemical_compound ,chemistry ,visual_art ,Botany ,visual_art.visual_art_medium ,Penicillium citrinum - Abstract
The aim of this study was to isolate fungi able to produce pigments. Fifty strains were isolated from the Amazon soil by the conventional technique of serial dilution. Submerged fermentation was performed in Czapeck broth in order to select strains able to synthesise pigments. Five strains were able to produce pigments and were identified by sequencing the rDNA (ITS regions). These fungi were identified as Penicillium sclerotiorum 2AV2, Penicillium sclerotiorum 2AV6, Aspergillus calidoustus 4BV13, Penicillium citrinum 2AV18 and Penicillium purpurogenum 2BV41. P. sclerotiorum 2AV2 produced intensely coloured pigments and were therefore selected for chemical characterisation. NMR identified the pigment as sclerotiorin. In this work, the influence of nutrients on sclerotiorin yield was also studied and it was verified that rhamnose and peptone increased production when used separately. These results indicate that Amazonian fungi bioprospecting is a viable means to search for new sources of natural dyes. more...
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- 2014
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4. Availability and morphological characteristics of endophytic fungi held in different methods of preservation
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Amaury dos Santos Bentes, Ana Karla Lima Freire, Érica SimplÃcio de Souza, Liliane Coelho da Rocha, Ivanete de Lima Sampaio, Luciana Fujimoto Botineli, João Braga de Souza, and Alita Moura de Lima
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0301 basic medicine ,Fusarium ,Aspergillus ,Preservation methods ,biology ,Microorganism ,030106 microbiology ,General Engineering ,General Physics and Astronomy ,General Medicine ,Contamination ,biology.organism_classification ,General Biochemistry, Genetics and Molecular Biology ,Plant use of endophytic fungi in defense ,03 medical and health sciences ,Horticulture ,030104 developmental biology ,Distilled water ,Mycology ,General Agricultural and Biological Sciences - Abstract
Maintenance of microorganisms in mycology is of fundamental importance for retrospective and prospective studies that focus on their biology, etiology and epidemiology. The aim of this study was to evaluate the viability, contamination and morphological changes of endophytic fungi maintained under different preservation methods. We evaluated five preservation methods; constant sub-culturing, preservation under mineral oil, under distilled water, preservation at -20°C and storage at -70°C. Every 50 days, we evaluated the viability, purity and macro-micro morphology of the cultures. The main results are as follows: constant sub-culturing and preservation under distilled water have allowed the viability of all strains during the study period. Preservation in mineral oil resulted in the contamination of the strain Aspergillus F45 and micro-morphological modification of the Fusarium LU5 culture after 100 days of preservation. Preservation at -70°C caused macro-morphological changes in Fusarium LU6 after 100 days. Under the experimental conditions and the limited period of study (150 days) it was demonstrated that conservation under distilled water was the most appropriate form of preservation of endophytic microorganisms. Key words: Endophytes, preservation methods, morphological characteristics. more...
- Published
- 2016
5. Availability and morphological characteristics of endophytic fungi held in different methods of preservation
- Author
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Ana, Karla Lima Freire, primary, Amaury, dos Santos Bentes, additional, Ivanete, de Lima Sampaio, additional, Alita, Moura de Lima, additional, Luciana, Fujimoto Botineli, additional, Liliane, Coelho da Rocha, additional, João, Braga de Souza, additional, and Érica, Simplício de Souza, additional more...
- Published
- 2016
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6. Mansonella ozzardi (Nematoda: Onchocercidae) in the riverine population of the Tefé River, State of Amazonia, Brazil
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Alita Moura de Lima, Cristóvão Alves da Costa, Jansen Fernandes Medeiros, and Felipe Arley Costa Pessoa
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Male ,Rural Population ,Veterinary medicine ,Prevalence ,Clinical Female ,Child ,Aged, 80 and over ,education.field_of_study ,biology ,Amazon rainforest ,Ecology ,Blood Smear ,Mansonelliasis ,Middle Aged ,Aged, 80 And Over ,Filariasis ,Infectious Diseases ,Geography ,Mansonella ozzardi ,Child, Preschool ,Female ,Pilot Study ,Brazil ,Human ,Oil and natural gas ,Microbiology (medical) ,Adult ,Tefé River ,lcsh:Arctic medicine. Tropical medicine ,Adolescent ,lcsh:RC955-962 ,Population ,Amazonas ,Young Adult ,Rivers ,medicine ,Animals ,Humans ,education ,Aged ,Animal ,Brasil ,Mansonella ,Onchocercidae ,biology.organism_classification ,medicine.disease ,Blood smear ,Parasitology - Abstract
Introduction: This study assessed the prevalence of Mansonella ozzardi in riverine communities of the Tefé River, Amazonas, Brazil. Methods: The prevalence of M. ozzardi was estimated by microscopic examination of thick blood smears. Results: The M. ozzardi prevalence rate was 6.3% (19/300). Filarial infection was found in 8 of the 11 communities surveyed, with prevalence rates varying from 2.5% to 22.2%. Conclusions: Tefé is a region of oil and natural gas exploration, in which there is a high turnover of workers. Migration patterns may facilitate the dissemination of mansonelliasis to other regions. more...
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- 2012
7. Effects of culture filtrates of endophytic fungi obtained from Piper aduncum L. on the growth of Mycobacterium tuberculosis
- Author
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Clarice Maia Carvalho, João Vicente Braga de Souza, Francisco Célio Maia Chaves, Alita Moura de Lima, Ana Cláudia Alves Cortez, Julia Ignez Salem, and Valdir Florêncio da Veiga Junior
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Tuberculosis ,Deuteromycetes ,Fungal Morphology ,Culture Filtrate ,Piper Aduncum ,Fungus Culture ,Biology ,Bacterial growth ,Applied Microbiology and Biotechnology ,Plant use of endophytic fungi in defense ,Plant Stem ,Microbiology ,Mycobacterium tuberculosis ,Tuberculosis diagnosis ,stimulation growth ,medicine ,Controlled Study ,Plant Leaf ,Endophytic Fungi ,Fungus ,Piper aduncum ,M. Tuberculosis ,Bioprocessing ,Plant Extracts ,Bacterial Growth ,Microbial Growth ,Fungi ,Piperaceae ,Fungus Isolation ,medicine.disease ,Antimicrobial ,biology.organism_classification ,Nonhuman ,antagonism ,Culture Media ,tuberculosis ,Endophytic Fungus ,Bioassay ,Bioprocess ,Bioprocesses ,Extracellular Metabolites ,Mycobacterium Tuberculosis ,Biotechnology - Abstract
Normal 0 21 false false false ES-CL X-NONE X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabla normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} Substances that inhibit the growth of Mycobacterium tuberculosis could potentially be used as antibiotics. These substances could also be added to test culture media to improve the speed of tuberculosis diagnosis. The aim of this work was to investigate the influence of culture filtrates of endophytic fungi isolated from P. aduncum L. on the growth of M. tuberculosis . To achieve this objective, the following methodology was used: a) endophytic fungi were isolated from the leaves and stems of P. aduncum L.; b) the isolated fungi were submitted to submerged bioprocessing; c) culture filtrates from the bioprocess were assayed to evaluate their effect on the growth of M. tuberculosis . We isolated 315 fungal types, which represented 85 morphologies, from different parts of P. aduncum L. The bioassays were performed on 82 culture filtrates and 6 plant extracts and resulted in the detection of 1 culture filtrate that stimulated the growth of M. tuberculosis and 15 that inhibited microbial growth. None of the phytochemical extracts had an effect on the growth of M. tuberculosis . In conclusion, we observed that the endophytic fungi isolated from P. aduncum L. (Piperaceae) produced extracellular metabolites (present in the culture filtrate) that affect the growth of M. tuberculosis . These compounds have the potential to be used as antimicrobials or in the diagnosis of tuberculosis. more...
- Published
- 2011
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