6,083 results on '"Allergic inflammation"'
Search Results
2. Allergy and Immunology Natural History Study
- Published
- 2024
3. Targeted inhibition of m6A demethylase FTO by FB23 attenuates allergic inflammation in the airway epithelium.
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Lian, Zexuan, Chen, Ruchong, Xian, Mo, Huang, Peiying, Xu, Jiahan, Xiao, Xiaojun, Ning, Xiaoping, Zhao, Jin, Xie, Jianlei, Duan, Jielin, Li, Bizhou, Wang, Wanjun, Shi, Xu, Wang, Xinru, Jia, Nan, Chen, Xuepeng, Li, Jing, and Yang, Zhaowei
- Abstract
Epithelial cells play a crucial role in asthma, contributing to chronic inflammation and airway hyperresponsiveness. m6A modification, which involves key proteins such as the demethylase fat mass and obesity‐associated protein (FTO), is crucial in the regulation of various diseases, including asthma. However, the role of FTO in epithelial cells and the development of asthma remains unclear. In this study, we investigated the demethylase activity of FTO using a small‐molecule inhibitor FB23 in epithelial cells and allergic inflammation in vivo and in vitro. We examined the FTO‐regulated transcriptome‐wide m6A profiling by methylated RNA immunoprecipitation sequencing (MeRIP‐seq) and RNA‐seq under FB23 treatment and allergic inflammation conditions. Immunofluorescence staining was performed to assess the tissue‐specific expression of FTO in asthmatic bronchial mucosa. We demonstrated that FB23 alleviated allergic inflammation in IL‐4/IL‐13‐treated epithelial cells and house dust mite (HDM)‐induced allergic airway inflammation mouse model. The demethylase activity of FTO contributed to the regulation of TNF‐α signaling via NF‐κB and epithelial–mesenchymal transition‐related pathways under allergic inflammation conditions in epithelial cells. FTO was expressed in epithelial, submucosal gland, and smooth muscle cells in human bronchial mucosa. In conclusion, FB23‐induced inhibition of FTO alleviates allergic inflammation in epithelial cells and HDM‐induced mice, potentially through diverse cellular processes and epithelial–mesenchymal transition signaling pathways, suggesting that FTO is a potential therapeutic target in asthma management. [ABSTRACT FROM AUTHOR]
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- 2024
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4. Involvement of Muscarinic M3 Receptor in the Development of M2 Macrophages in Allergic Inflammation.
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Jinno, Megumi, Ohta, Shin, Mikuni, Hatsuko, Uno, Tomoki, Uchida, Yoshitaka, Manabe, Ryo, Miyata, Yoshito, Homma, Tetsuya, Watanabe, Yoshio, Kusumoto, Sojiro, Suzuki, Shintaro, Tanaka, Akihiko, and Sagara, Hironori
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MUSCARINIC receptors , *MONONUCLEAR leukocytes , *ASTHMATICS , *BONE marrow , *FLOW cytometry - Abstract
Introduction: The muscarinic M3 receptor antagonist, tiotropium, has a bronchodilatory effect on asthma patients. Additionally, tiotropium inhibits allergic airway inflammation and remodeling in a murine asthma model. However, the underlying mechanisms of this M3 receptor antagonist remain unclear. Therefore, we investigated the effect of muscarinic M3 receptor blockage on M2 macrophage development during allergic airway inflammation. Methods: BALB/c mice were sensitized and challenged with ovalbumin to develop a murine model of allergic airway inflammation mimicking human atopic asthma. During the challenge phase, mice were treated with or without tiotropium. Lung cells were isolated 24 h after the last treatment and gated using CD68-positive cells. Relm-α and Arginase-1 (Arg1) (M2 macrophage markers) expression was determined by flow cytometry. Mouse bone marrow mononuclear cell-derived macrophages (mBMMacs) and human peripheral blood mononuclear cells (PBMCs)-derived macrophages were stimulated with IL-4 and treated with a muscarinic M3 receptor antagonist in vitro. Results: The total cells, eosinophils, and IL-5 and IL-13 levels in BAL fluids were markedly decreased in the asthma group treated with tiotropium compared to that in the untreated asthma group. The Relm-α and Arg1 expression in macrophages was reduced considerably in the asthma group treated with tiotropium compared to that in the untreated asthma group, suggesting that the development of M2 macrophages was inhibited by muscarinic M3 receptor blockage. Additionally, muscarinic M3 receptor blockage in vitro significantly inhibited M2 macrophage development in both mBMMacs- and PBMCs-derived macrophages. Conclusions: Muscarinic M3 receptor blockage inhibits M2 macrophage development and prevents allergic airway inflammation. Moreover, muscarinic M3 receptors might be involved in the differentiation of immature macrophages into M2 macrophages. [ABSTRACT FROM AUTHOR]
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- 2024
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5. TH2-driven manifestations of inborn errors of immunity.
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James, Alyssa E., Abdalgani, Manar, Khoury, Paneez, Freeman, Alexandra F., and Milner, Joshua D.
- Abstract
Monogenic lesions in pathways critical for effector functions responsible for immune surveillance, protection against autoinflammation, and appropriate responses to allergens and microorganisms underlie the pathophysiology of inborn errors of immunity (IEI). Variants in cytokine production, cytokine signaling, epithelial barrier function, antigen presentation, receptor signaling, and cellular processes and metabolism can drive autoimmunity, immunodeficiency, and/or allergic inflammation. Identification of these variants has improved our understanding of the role that many of these proteins play in skewing toward T H 2-related allergic inflammation. Early-onset or atypical atopic disease, often in conjunction with immunodeficiency and/or autoimmunity, should raise suspicion for an IEI. This becomes a diagnostic dilemma if the initial clinical presentation is solely allergic inflammation, especially when the prevalence of allergic diseases is becoming more common. Genetic sequencing is necessary for IEI diagnosis and is helpful for early recognition and implementation of targeted treatment, if available. Although genetic evaluation is not feasible for all patients with atopy, identifying atopic patients with molecular immune abnormalities may be helpful for diagnostic, therapeutic, and prognostic purposes. In this review, we focus on IEI associated with T H 2-driven allergic manifestations and classify them on the basis of the affected molecular pathways and predominant clinical manifestations. [ABSTRACT FROM AUTHOR]
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- 2024
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6. Notch 2 signaling contributes to intestinal eosinophil adaptations in steady state and tissue burden following oral allergen challenge.
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Schworer, Stephen A, Olbrich, Courtney L, Larsen, Leigha D, Howard, Emily, Liu, Linying, Koyama, Kenya, and Spencer, Lisa A
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EOSINOPHILS ,ALLERGIES ,HUMAN phenotype ,HOMEOSTASIS ,GASTROINTESTINAL diseases - Abstract
Eosinophils not only function as inflammatory effectors in allergic diseases, but also contribute to tissue homeostasis in steady state. Emerging data are revealing tissue eosinophils to be adaptive cells, imprinted by their local tissue microenvironment and exhibiting distinct functional phenotypes that may contribute to their homeostatic vs. inflammatory capacities. However, signaling pathways that regulate eosinophil tissue adaptations remain elusive. Notch signaling is an evolutionarily conserved pathway that mediates differential cell fate programming of both pre- and postmitotic immune cells. This study investigated a role for notch receptor 2 signaling in regulating eosinophil functions and tissue phenotype in both humans and mice. Notch 2 receptors were constitutively expressed and active in human blood eosinophils. Pharmacologic neutralization of notch 2 in ex vivo stimulated human eosinophils altered their activated transcriptome and prevented their cytokine-mediated survival. Genetic ablation of eosinophil-expressed notch 2 in mice diminished steady-state intestine-specific eosinophil adaptations and impaired their tissue retention in a food allergic response. In contrast, notch 2 had no effect on eosinophil phenotype or tissue inflammation within the context of allergic airways inflammation, suggesting that notch 2–dependent regulation of eosinophil phenotype and function is specific to the gut. These data reveal notch 2 signaling as a cell-intrinsic mechanism that contributes to eosinophil survival, function, and intestine-specific adaptations. The notch 2 pathway may represent a viable strategy to reprogram eosinophil functional phenotypes in gastrointestinal eosinophil-associated diseases. [ABSTRACT FROM AUTHOR]
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- 2024
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7. Nur77 Mediates Anaphylaxis by Regulating miR-21a
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Hyein Jo, Jaewhoon Jeoung, Kyeonghee Shim, and Dooil Jeoung
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allergic inflammation ,C-JUN ,miR-21a ,Nur77 ,Biology (General) ,QH301-705.5 - Abstract
Nur77 belongs to the NR4A subfamily of orphan nuclear hormone receptors. It has been shown to play important roles in metabolism, cancer progression, cellular differentiation, and the regulation of immune process. However, there has yet to be research reporting on the role of Nur77 in allergic inflammations such as anaphylaxis. This study aimed to identify molecules that could mediate allergic inflammations. To this end, we performed RNA sequencing analysis employing bone marrow-derived mast cells (BMMCs). Antigen (DNP-HSA) stimulation increased the expression levels of transcription factors such as Nr4a3 (NOR1), Nr4a1 (Nur77), and Nr4a2 (Nurr1). We focused our study on Nur77. Antigen stimulation increased the expression of Nur77 in a time- and dose-dependent manner in rat basophilic leukemia cells (RBL2H3). The downregulation of Nur77 prevented both antigen-induced increase in β-hexosaminidase activity as well as hallmarks of allergic reactions such as HDAC3, COX2, and MCP1 in RBL2H3 cells. Nur77 was necessary for both passive cutaneous anaphylaxis (PCA) and passive systemic anaphylaxis (PSA). TargetScan analysis predicted that miR-21a would be a negative regulator of Nur77. miR-21a mimic negatively regulated PCA and PSA by inhibiting the hallmarks of allergic reactions. ChIP assays showed that c-JUN could bind to the promoter sequences of Nur77. Antigen stimulation increased the expression of c-JUN in RBL2H3 cells. Altogether, our findings demonstrate the regulatory role played by Nur77-miR-21a loop in allergic inflammations such as anaphylaxis, making this the first report to present the role played by Nur77 in an allergic inflammation. Our results suggest that Nur77 and miR-21 might serve as targets for developing anti-allergy drugs.
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- 2024
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8. Allergic inflammation triggers dyslipidemia via IgG signalling.
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Fernández‐Gallego, Nieves, Castillo‐González, Raquel, Moreno‐Serna, Lucía, García‐Cívico, Antonio J., Sánchez‐Martínez, Elisa, López‐Sanz, Celia, Fontes, Ana Luiza, Pimentel, Lígia L., Gradillas, Ana, Obeso, David, Neuhaus, René, Ramírez‐Huesca, Marta, Ruiz‐Fernández, Ignacio, Nuñez‐Borque, Emilio, Carrasco, Yolanda R., Ibáñez, Borja, Martín, Pilar, Blanco, Carlos, Barbas, Coral, and Barber, Domingo
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Background Methods Results Conclusion Allergic diseases begin early in life and are often chronic, thus creating an inflammatory environment that may precede or exacerbate other pathologies. In this regard, allergy has been associated to metabolic disorders and with a higher risk of cardiovascular disease, but the underlying mechanisms remain incompletely understood.We used a murine model of allergy and atherosclerosis, different diets and sensitization methods, and cell‐depleting strategies to ascertain the contribution of acute and late phase inflammation to dyslipidemia. Untargeted lipidomic analyses were applied to define the lipid fingerprint of allergic inflammation at different phases of allergic pathology. Expression of genes related to lipid metabolism was assessed in liver and adipose tissue at different times post‐allergen challenge. Also, changes in serum triglycerides (TGs) were evaluated in a group of 59 patients ≥14 days after the onset of an allergic reaction.We found that allergic inflammation induces a unique lipid signature that is characterized by increased serum TGs and changes in the expression of genes related to lipid metabolism in liver and adipose tissue. Alterations in blood TGs following an allergic reaction are independent of T‐cell‐driven late phase inflammation. On the contrary, the IgG‐mediated alternative pathway of anaphylaxis is sufficient to induce a TG increase and a unique lipid profile. Lastly, we demonstrated an increase in serum TGs in 59 patients after undergoing an allergic reaction.Overall, this study reveals that IgG‐mediated allergic inflammation regulates lipid metabolism. [ABSTRACT FROM AUTHOR]
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- 2024
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9. Targeting NF-κB Signaling: Selected Small Molecules Downregulate Pro-Inflammatory Cytokines in Both Food Allergen and LPS-Induced Inflammation.
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Zlatanova, Milena, Nešić, Andrijana, Trbojević-Ivić, Jovana, Četić, Danilo, and Gavrović-Jankulović, Marija
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SMALL molecules , *ALLERGENS , *CYTOKINES , *INFLAMMATION , *LIPOPOLYSACCHARIDES , *LAURIC acid - Abstract
Although inflammation is primarily a protective response guarding the human body, it can result in a variety of chronic diseases such as allergies, auto-immune, cardiovascular diseases, and cancer. In NF-κB-mediated inflammation, many small molecules and food compounds characterized as nutraceuticals have shown positive effects associated with immunomodulatory properties. We investigated the effects of selected bioactive small molecules, commonly found in food components, vanillyl alcohol (VA) and lauric acid (LA), on different cell lines exposed to pro-inflammatory stimuli, lipopolysaccharide (LPS), and the food allergen actinidin (Act d 1). Pro-inflammatory cytokines were downregulated in response to both VA and LA, and this downregulation was caused by a decrease in the activation of the NF-κB pathway and the translocation of p65, the pathway's major component. Small nutraceutical molecules, VA and LA, showed not only inhibition of the pro-inflammatory cytokines, but also inhibition of the NF-κB activation, and reduced translocation of the p65 component. The present study may contribute to the therapeutic use of these molecules for various inflammatory diseases, which have in common an increased expression of pro-inflammatory cytokines and NF-κB-mediated inflammation. [ABSTRACT FROM AUTHOR]
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- 2024
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10. 5-Hydroxymethylfurfural Ameliorates Allergic Inflammation in HMC-1 Cells by Inactivating NF-κB and MAPK Signaling Pathways.
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Hu, Pan, Zhang, Zhuo, Yu, Xiaolin, and Wang, Yinglin
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OVALBUMINS , *IMMUNOGLOBULIN E , *MITOGEN-activated protein kinases , *CELLULAR signal transduction , *ALANINE aminotransferase , *MAST cells , *INFLAMMATION , *CALCIUM channels - Abstract
Allergic inflammation is the foundation of multiple allergic disorders, such as allergic rhinitis and asthma. Mast cells are effector cells that initiate inflammatory response. 5-hydroxymethylfurfural (5-HMF), a furfural compound, is the heat-processed product of various fruit, foods, drinks, as well as some Chinese herbal medicines. 5-HMF was previously reported to inhibit mast cell activation. Our study aimed to explore the functions of 5-HMF in both phorbol 12-mystate 13-acetate (PMA) plus calcium ionophore (A23187)-induced allergic inflammation in human mast cell line HMC-1 and ovalbumin (OVA)-induced asthma mouse models. HMC-1 cells were pretreated with 5-HMF and then stimulated by PMA+A23187. The cytotoxicity of 5-HMF on HMC-1 cells was evaluated by MTT assay. Histamine content in cell supernatants was measured by the o-phthaldialdehyde spectrofluorometric procedure. Intracellular calcium was determined using the fluorescent dye Fura-2AM. The production and expression of pro-inflammatory cytokines were detected by ELISA and RT-qPCR. Caspase-1 colorimetric assay was employed to examine the enzymatic activity of caspase-1. Asthma mouse models were induced by OVA sensitization. The bronchoalveolar lavage fluid (BALF) and blood samples were collected for the detection of total and differential cell count as well as aspartate aminotransferase (AST), alanine aminotransferase (ALT), OVA-immunoglobulin E (OVA-IgE), OVA-immunoglobulin G1 (OVA-IgG1), and pro-inflammatory cytokine levels. The left lung of mouse was dissected for histopathological examination by hematoxylin and eosin (H&E) staining. The protein expression of pro-caspase-1 and the phosphorylation of NF-κB and MAPK pathway-associated molecules were assessed by Western blotting. Our findings revealed that 5-HMF efficiently suppressed the PMA+A23187-induced enhancement in histamine production and intracellular calcium in HMC-1 cells. Pro-inflammatory cytokine production and expression in HMC-1 cells were elevated after PMA plus A23187 stimulation, which, however, were inhibited by pretreatment of 5-HMF. Additionally, 5-HMF suppressed the activity of caspase-1 and the phosphorylation of NF-κB and MAPK-associated molecules including p65 NF-κB, p38 MAPK, ERK, and JNK in HMC-1 cells. In vivo experiments demonstrated that 5-HMF treatment reduced the lung/body weight index and total and differential (macrophages, neutrophils, lymphocytes, and eosinophils) cell counts in BALF of asthmatic mice, but exerted no influence on serum AST and ALT levels. Besides, 5-HMF reduced serum OVA-IgE and OVA-IgG1 levels, mitigated lung inflammation, and inhibited the NF-κB and MAPK signaling pathways in asthma mouse models. 5-HMF mitigates allergic inflammation in asthma by inactivating caspase-1 and NF-κB and MAPK signaling pathways. [ABSTRACT FROM AUTHOR]
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- 2024
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11. Gonadal sex and chromosome complement influence the gut microbiome in a mouse model of allergic airway inflammation.
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Ekpruke, Carolyn Damilola, Alford, Rachel, Parker, Erik, and Silveyra, Patricia
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SEX chromosomes , *GUT microbiome , *X chromosome , *Y chromosome , *LABORATORY mice , *ANIMAL disease models , *BIOMES - Abstract
Evidence abounds that gut microbiome components are associated with sex disparities in the immune system. However, it remains unclear whether the observed sex disparity in asthma incidence is associated with sex-dependent differences in immune-modulating gut microbiota, and/or its influence on allergic airway inflammatory processes. Using a mouse model of house dust mite (HDM)-induced allergic inflammation and the four core genotypes (FCGs) model, we have previously reported sex differences in lung inflammatory phenotypes. Here, we investigated associations of gut microbiomes with these phenotypes by challenging FCG mice [mouse with female sex chromosome and male gonad (XXM), mouse with female sex chromosome and female gonad (XXF), mouse with male sex chromosome and male gonad (XYM), and mouse with male sex chromosome and female gonad (XYF); n = 7/group] with HDM (25 μg) or PBS intranasally for 5 wk and collecting fecal samples. We extracted fecal DNA and analyzed the 16S microbiome via Targeted Metagenomic Sequencing. We compared α and β diversity across genotypes and assessed the Firmicutes/Bacteroidetes (F/B) ratio. When comparing baseline and after exposure for the FCG, we found that the gut F/B ratio was only increased in the XXM genotype. We also found that α diversity was significantly increased in all FCG mice upon HDM challenge, with the highest increase in the XXF, and the lowest in the XXM genotypes. Similarly, β diversity of the microbial community was also affected by challenge in a gonad- and chromosome-dependent manner. In summary, our results indicated that HDM treatment, gonads, and sex chromosomes significantly influence the gut microbial community composition. We concluded that allergic lung inflammation may be affected by the gut microbiome in a sex-dependent manner involving both hormonal and genetic influences. NEW & NOTEWORTHY: Recently, the gut microbiome and its role in chronic respiratory disease have been the subject of extensive research and the establishment of its involvement in immune functions. Using the FCG mouse model, our findings revealed the influence of gonads and sex chromosomes on the microbial community structure before and after exposure to HDM. Our data provide a potential new avenue to better understand mediators of sex disparities associated with allergic airway inflammation. [ABSTRACT FROM AUTHOR]
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- 2024
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12. Keratinocyte-derived small extracellular vesicles supply antigens for CD1a-resticted T cells and promote their type 2 bias in the context of filaggrin insufficiency.
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Kobiela, Adrian, Hewelt-Belka, Weronika, Frąckowiak, Joanna E., Kordulewska, Natalia, Hovhannisyan, Lilit, Bogucka, Aleksandra, Etherington, Rachel, Piróg, Artur, Dapic, Irena, Gabrielsson, Susanne, Brown, Sara J., Ogg, Graham S., and Gutowska-Owsiak, Danuta
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T cells ,FILAGGRIN ,EXTRACELLULAR vesicles ,MICROPHYSIOLOGICAL systems ,UNSATURATED fatty acids ,T cell receptors - Abstract
Introduction: Exosome-enriched small extracellular vesicles (sEVs) are nanosized organelles known to participate in long distance communication between cells, including in the skin. Atopic dermatitis (AD) is a chronic inflammatory skin disease for which filaggrin (FLG) gene mutations are the strongest genetic risk factor. Filaggrin insufficiency affects multiple cellular function, but it is unclear if sEV-mediated cellular communication originating from the affected keratinocytes is also altered, and if this influences peptide and lipid antigen presentation to T cells in the skin. Methods: Available mRNA and protein expression datasets from filaggrininsufficient keratinocytes (shFLG), organotypic models and AD skin were used for gene ontology analysis with FunRich tool. sEVs secreted by shFLG and control shC cells were isolated from conditioned media by differential centrifugation. Mass spectrometry was carried out for lipidomic and proteomic profiling of the cells and sEVs. T cell responses to protein, peptide, CD1a lipid antigens, as well as phospholipase A2-digested or intact sEVs were measured by ELISpot and ELISA. Results: Data analysis revealed extensive remodeling of the sEV compartment in filaggrin insufficient keratinocytes, 3D models and the AD skin. Lipidomic profiles of shFLGsEV showed a reduction in the long chain (LCFAs) and polyunsaturated fatty acids (PUFAs; permissive CD1a ligands) and increased content of the bulky headgroup sphingolipids (non-permissive ligands). This resulted in a reduction of CD1a-mediated interferon-g T cell responses to the lipids liberated from shFLGgenerated sEVs in comparison to those induced by sEVs from control cells, and an increase in interleukin 13 secretion. The altered sEV lipidome reflected a generalized alteration in the cellular lipidome in filaggrin-insufficient cells and the skin of AD patients, resulting from a downregulation of key enzymes implicated in fatty acid elongation and desaturation, i.e., enzymes of the ACSL, ELOVL and FADS family. Discussion: We determined that sEVs constitute a source of antigens suitable for CD1a-mediated presentation to T cells. Lipids enclosed within the sEVs secreted on the background of filaggrin insufficiency contribute to allergic inflammation by reducing type 1 responses and inducing a type 2 bias from CD1a-restricted T cells, thus likely perpetuating allergic inflammation in the skin. [ABSTRACT FROM AUTHOR]
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- 2024
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13. Downregulation of lncRNA CDKN2B-AS1 attenuates inflammatory response in mice with allergic rhinitis by regulating miR-98-5p/SOCS1 axis.
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Bangyu Deng, Yunxia zhao, and Jisheng Liu
- Abstract
Long non-coding RNA cyclin-dependent kinase inhibitor 2B antisense RNA 1 (CDKN2B-AS1) in various diseases has been verified. However, the underlying mechanism of CDKN2B-AS1 contributes to the development of allergic rhinitis (AR) remains unknown. To evaluate the impact of CDKN2B-AS1 on AR, BALB/c mice were sensitized by intraperitoneal injection of normal saline containing ovalbumin (OVA) and calmogastrin to establish an AR model. Nasal rubbing and sneezing were documented after the final OVA treatment. The concentrations of IgE, IgG1, and inflammatory elements were quantified using ELISA. Hematoxylin and eosin (H&E) staining and immunofluorescence were used to assess histopathological variations and tryptase expression, respectively. StarBase, TargetScan and luciferase reporter assays were applied to predict and confirm the interactions among CDKN2B-AS1, miR-98-5p, and SOCS1. CDKN2B-AS1, miR-98-5p, and SOCS1 levels were assessed by quantitative real-time PCR (qRT-PCR) or western blotting. Our results revealed that CDKN2B-AS1 was obviously over-expressed in the nasal mucosa of AR patients and AR mice. Down-regulation of CDKN2B-AS1 significantly decreased nasal rubbing and sneezing frequencies, IgE and IgG1 concentrations, and cytokine levels. Furthermore, down-regulation of CDKN2B-AS1 also relieved the pathological changes in the nasal mucosa, and the infiltration of eosinophils and mast cells. Importantly, these results were reversed by the miR-98-5p inhibitor, whereas miR-98-5p directly targeted CDKN2B-AS1, and miR-98-5p negatively regulated SOCS1 level. Our findings demonstrate that down-regulation of CDKN2B-AS1 improves allergic inflammation and symptoms in a murine model of AR through the miR-98-5p/SOCS1 axis, which provides new insights into the latent functions of CDKN2B-AS1 in AR treatment. [ABSTRACT FROM AUTHOR]
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- 2024
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14. Theory of Lipid Metabolism Disorders in Rhinitis and Asthma (Lipid Droplets)
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Zheng, Shaohua, Guo, Yijia, Wu, Zhaoyan, and Cheng, Jing
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- 2024
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15. Human germline gain-of-function in STAT6: from severe allergic disease to lymphoma and beyond.
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ALLERGIES , *STAT proteins , *ATOPY , *GENOME-wide association studies , *GERM cells , *DRUG allergy - Abstract
Primary atopic disorders (PADs) are a subset of monogenic inborn errors of immunity (IEIs) characterized by severe allergic diseases. Recently, a new PAD was discovered that was caused by germline heterozygous gain-of-function (GOF) variants in human STAT6. Signal transducer and activator of transcription (STAT)-6-GOF disease was recently diagnosed in 21 individuals with severe allergic disease from birth. These patients presented with severe treatment-resistant atopic dermatitis, eosinophilic gastrointestinal disease, skin and respiratory infections, and other non-allergic manifestations. This discovery follows decades of fundamental biological research into STAT6, establishing it as a molecule that is central to T helper type 2 (T H 2) immunity. This builds on a large body of knowledge, integrating both mouse models and genome-wide association studies, linking STAT6 function with allergic disease. The mechanism of STAT6-GOF disease emphasizes the central role of enhanced STAT6 activity, via increased phosphorylation, delayed dephosphorylation, as well as increased nuclear translocation and transcription of STAT6 targets and T H 2 polarization. Early results indicate that precision medicine approaches targeting the IL4R/Janus kinase (JAK/)STAT6 pathway, particularly dupilumab and JAK inhibitors, might be effective in treating STAT6-GOF disease. Germline heterozygous GOF STAT6 variants have been recently discovered to cause a broad and severe human clinical phenotype of asthma, atopic dermatitis, food and drug allergies, eosinophilic gastrointestinal disease, as well as skeletal and vascular anomalies. Moving forward, as more patients are found to have STAT6-GOF disease and the mechanisms of disease are fully defined, we may appreciate the full clinical phenotype and optimal treatment approaches for this newfound primary atopic disorder. Signal transducer and activator of transcription (STAT)-6 is a transcription factor central to pro-allergic immune responses, although the function of human STAT6 at the whole-organism level has long remained unknown. Germline heterozygous gain-of-function (GOF) rare variants in STAT6 have been recently recognized to cause a broad and severe clinical phenotype of early-onset, multi-system allergic disease. Here, we provide an overview of the clinical presentation of STAT6-GOF disease, discussing how dysregulation of the STAT6 pathway causes severe allergic disease, and identifying possible targeted treatment approaches. Finally, we explore the mechanistic overlap between STAT6-GOF disease and other monogenic atopic disorders, and how this group of inborn errors of immunity (IEIs) powerfully inform our fundamental understanding of common human allergic disease. [ABSTRACT FROM AUTHOR]
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- 2024
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16. Transcriptomics analysis of allergen-induced inflammatory gene expression in the Four-Core Genotype mouse model.
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Ekpruke, Carolyn Damilola, Alford, Rachel, Rousselle, Dustin, Babayev, Maksat, Sharma, Shikha, Commodore, Sarah, Buechlein, Aaron, Rusch, Douglas B., and Silveyra, Patricia
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GENE expression , *GENE expression profiling , *TRANSCRIPTOMES , *LABORATORY mice , *HOUSE dust mites , *GENOTYPES , *SEX chromosomes , *IMMUNOGLOBULIN E - Abstract
Sex differences in allergic inflammation have been reported, but the mechanisms underlying these differences remain unknown. Contributions of both sex hormones and sex-related genes to these mechanisms have been previously suggested in clinical and animal studies. Here, Four-Core Genotypes (FCG) mouse model was used to study the inflammatory response to house dust mite (HDM) challenge and identify differentially expressed genes (DEGs) and regulatory pathways in lung tissue. Briefly, adult mice (8–10 wk old) of the FCG (XXM, XXF, XYM, XYF) were challenged intranasally with 25 μg of HDM or vehicle (PBS-control group) 5 days/wk for 5 wk (n ¼ 3/10 group). At 72 h after the last exposure, we analyzed the eosinophils and neutrophils in the bronchoalveolar lavage (BAL) of FCG mice. We extracted lung tissue and determined DEGs using Templated Oligo-Sequencing (TempO-Seq). DEG analysis was performed using the DESeq2 package and gene enrichment analysis was done using Ingenuity Pathway Analysis. A total of 2,863 DEGs were identified in the FCG. Results revealed increased eosinophilia and neutrophilia in the HDM-treated group with the most significantly expressed genes in XYF phenotype and a predominant effect of female hormones vs. chromosomes. Regardless of the sex hormones, mice with female chromosomes had more downregulated genes in the HDM group but this was reversed in the control group. Interestingly, genes associated with inflammatory responses were overrepresented in the XXM and XYF genotypes treated with HDM. Sex hormones and chromosomes contribute to inflammatory responses to HDM challenge, with female hormones exerting a predominant effect mediated by inflammatory DEGs. NEW & NOTEWORTHY Gene expression profiling helps to provide deep insight into the global view of disease-related mechanisms and responses to therapy. Using the Four-Core Genotype mouse model, our findings revealed the influence of sex hormones and sex chromosomes in the gene expression of lungs exposed to an aeroallergen (House Dust Mite) and identified sexspecific pathways to better understand sex disparities associated with allergic airway inflammation. [ABSTRACT FROM AUTHOR]
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- 2024
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17. Studies examining the role of IL-33 and IL1RL1 (ST2) in human ILC2 function
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Alshammari, Mohammed
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human ILC2 function ,IL-33 ,IL1RL1 (ST2) ,Asthma ,heterogeneous disease ,allergic inflammation ,flow cytometry ,ST2 antibodies ,ST2 expression in asthma ,thesis - Abstract
Asthma is a heterogeneous disease characterised by frequent airway obstruction in the lung resulting from allergic (atopic) or non-allergic (non-atopic) triggers. Allergic asthma is associated with airway inflammation and is characterised by the infiltration of inflammatory cells including eosinophils, mast cells and T-helper 2 cells (TH2). Several soluble mediators have been shown to play a critical role in the pathogenicity of allergic asthma. These mediators include the hallmark TH2 cytokines interleukin (IL)-4, IL-5 and IL-13 and the epithelial-derived cytokines IL-25, IL-33 and Thymic Stromal Lymphopoietin (TSLP). Until recently, it was believed that allergic inflammation is linked to the CD4+ TH2 cells and mast cells. However, the recently discovered group 2 innate lymphoid cells (ILC2s) have been found to play an important role in mucosal surfaces including airway tracts, as they have the capacity to produce TH2 cytokines including IL-4, IL-5 and IL-13 upon stimulation with IL-25, IL-33 and TSLP. Interleukin 1 receptor-like 1 (IL1RL1), also known as ST2, with its ligand IL33, induces allergic inflammation due to the release of type 2 cytokines. The biology of ST2 is complex because it is expressed in two forms, the soluble protein sST2, which down regulates TH2 responses, and the transmembrane form ST2 (ST2 or ST2L). Several studies have demonstrated that ST2 is expressed by ILC2s in mice; however, it is unclear from the literature whether human ILC2s express ST2. Therefore, the aims of this project were to validate antibodies to ST2 and to use validated antibodies to examine ST2 expression on human peripheral blood samples, in particular ILC2s. We hypothesised that ST2 may be upregulated on human ILC2s from asthmatic subjects compared to healthy controls. The results demonstrated that most of the commonly used ST2 antibodies were not specific to ST2, calling into question several of the published reports in the literature. Despite identifying an appropriate antibody for use in flow cytometry, it was not possible to complete the assessment of ST2 expression in asthma due to the COVID19 pandemic.
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- 2022
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18. Aspalathin, a Primary Rooibos Flavonoid, Alleviates Mast Cell-Mediated Allergic Inflammation by the Inhibition of FcεRI Signaling Pathway
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Kim, Yeyoung, Lee, Soyoung, Jin, Meiling, Choi, Young-Ae, Choi, Jin Kyeong, Kwon, Taeg Kyu, Khang, Dongwoo, and Kim, Sang-Hyun
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- 2024
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19. More airway smooth muscle in males versus females in a mouse model of asthma: A blessing in disguise?
- Author
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Rebecka Gill, Andrés Rojas‐Ruiz, Magali Boucher, Cyndi Henry, and Ynuk Bossé
- Subjects
airway hyperresponsiveness ,airway remodelling ,allergic inflammation ,Physiology ,QP1-981 - Abstract
Abstract Mouse models are helpful in unveiling the mechanisms underlying sex disparities in asthma. In comparison to their female counterparts, male mice are hyperresponsive to inhaled methacholine, a cardinal feature of asthma that contributes to its symptoms. The physiological details and the structural underpinnings of this hyperresponsiveness in males are currently unknown. Herein, BALB/c mice were exposed intranasally to either saline or house dust mite once daily for 10 consecutive days to induce experimental asthma. Twenty‐four hours after the last exposure, respiratory mechanics were measured at baseline and after a single dose of inhaled methacholine that was adjusted to trigger the same degree of bronchoconstriction in both sexes (it was twice as high in females). Bronchoalveolar lavages were then collected, and the lungs were processed for histology. House dust mite increased the number of inflammatory cells in bronchoalveolar lavages to the same extent in both sexes (asthma, P = 0.0005; sex, P = 0.96). The methacholine response was also markedly increased by asthma in both sexes (e.g., P = 0.0002 for asthma on the methacholine‐induced bronchoconstriction). However, for a well‐matched bronchoconstriction between sexes, the increase in hysteresivity, an indicator of airway narrowing heterogeneity, was attenuated in males for both control and asthmatic mice (sex, P = 0.002). The content of airway smooth muscle was not affected by asthma but was greater in males (asthma, P = 0.31; sex, P < 0.0001). These results provide further insights regarding an important sex disparity in mouse models of asthma. The increased amount of airway smooth muscle in males might contribute functionally to their greater methacholine response and, possibly, to their decreased propensity for airway narrowing heterogeneity.
- Published
- 2023
- Full Text
- View/download PDF
20. Malaria-induced bacteremia as a consequence of multiple parasite survival strategies
- Author
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Donnelly, Erinn, Van de Water, Judy, and Luckhart, Shirley
- Subjects
Medical Microbiology ,Biomedical and Clinical Sciences ,Clinical Sciences ,Prevention ,Rare Diseases ,Infectious Diseases ,Digestive Diseases ,Malaria ,Vector-Borne Diseases ,2.2 Factors relating to the physical environment ,Aetiology ,2.1 Biological and endogenous factors ,Infection ,Good Health and Well Being ,Mast cells ,Bacteremia ,Intestinal permeability ,Allergic inflammation ,Parasite transmission ,Microbiology ,Medical microbiology - Abstract
Globally, malaria continues to be an enormous public health burden, with concomitant parasite-induced damage to the gastrointestinal (GI) barrier resulting in bacteremia-associated morbidity and mortality in both adults and children. Infected red blood cells sequester in and can occlude the GI microvasculature, ultimately leading to disruption of the tight and adherens junctions that would normally serve as a physical barrier to translocating enteric bacteria. Mast cell (MC) activation and translocation to the GI during malaria intensifies damage to the physical barrier and weakens the immunological barrier through the release of enzymes and factors that alter the host response to escaped enteric bacteria. In this context, activated MCs release Th2 cytokines, promoting a balanced Th1/Th2 response that increases local and systemic allergic inflammation while protecting the host from overwhelming Th1-mediated immunopathology. Beyond the mammalian host, recent studies in both the lab and field have revealed an association between a Th2-skewed host response and success of parasite transmission to mosquitoes, biology that is evocative of parasite manipulation of the mammalian host. Collectively, these observations suggest that malaria-induced bacteremia may be, in part, an unintended consequence of a Th2-shifted host response that promotes parasite survival and transmission. Future directions of this work include defining the factors and mechanisms that precede the development of bacteremia, which will enable the development of biomarkers to simplify diagnostics, the identification of therapeutic targets to improve patient outcomes and better understanding of the consequences of clinical interventions to transmission blocking strategies.
- Published
- 2021
21. Deciphering the Interplay between the Epithelial Barrier, Immune Cells, and Metabolic Mediators in Allergic Disease
- Author
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Lea Ling-Yu Kan, Peiting Li, Sharon Sze-Man Hon, Andrea Yin-Tung Lai, Aixuan Li, Katie Ching-Yau Wong, Danqi Huang, and Chun-Kwok Wong
- Subjects
allergy ,eosinophils ,ILC2 ,lipid mediators ,allergic inflammation ,metabolites ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Chronic exposure to harmful pollutants, chemicals, and pathogens from the environment can lead to pathological changes in the epithelial barrier, which increase the risk of developing an allergy. During allergic inflammation, epithelial cells send proinflammatory signals to group 2 innate lymphoid cell (ILC2s) and eosinophils, which require energy and resources to mediate their activation, cytokine/chemokine secretion, and mobilization of other cells. This review aims to provide an overview of the metabolic regulation in allergic asthma, atopic dermatitis (AD), and allergic rhinitis (AR), highlighting its underlying mechanisms and phenotypes, and the potential metabolic regulatory roles of eosinophils and ILC2s. Eosinophils and ILC2s regulate allergic inflammation through lipid mediators, particularly cysteinyl leukotrienes (CysLTs) and prostaglandins (PGs). Arachidonic acid (AA)-derived metabolites and Sphinosine-1-phosphate (S1P) are significant metabolic markers that indicate immune dysfunction and epithelial barrier dysfunction in allergy. Notably, eosinophils are promoters of allergic symptoms and exhibit greater metabolic plasticity compared to ILC2s, directly involved in promoting allergic symptoms. Our findings suggest that metabolomic analysis provides insights into the complex interactions between immune cells, epithelial cells, and environmental factors. Potential therapeutic targets have been highlighted to further understand the metabolic regulation of eosinophils and ILC2s in allergy. Future research in metabolomics can facilitate the development of novel diagnostics and therapeutics for future application.
- Published
- 2024
- Full Text
- View/download PDF
22. Targeting NF-κB Signaling: Selected Small Molecules Downregulate Pro-Inflammatory Cytokines in Both Food Allergen and LPS-Induced Inflammation
- Author
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Milena Zlatanova, Andrijana Nešić, Jovana Trbojević-Ivić, Danilo Četić, and Marija Gavrović-Jankulović
- Subjects
NF-κB ,immunomodulation ,pro-inflammatory cytokines ,allergic inflammation ,vanillyl alcohol ,lauric acid ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Although inflammation is primarily a protective response guarding the human body, it can result in a variety of chronic diseases such as allergies, auto-immune, cardiovascular diseases, and cancer. In NF-κB-mediated inflammation, many small molecules and food compounds characterized as nutraceuticals have shown positive effects associated with immunomodulatory properties. We investigated the effects of selected bioactive small molecules, commonly found in food components, vanillyl alcohol (VA) and lauric acid (LA), on different cell lines exposed to pro-inflammatory stimuli, lipopolysaccharide (LPS), and the food allergen actinidin (Act d 1). Pro-inflammatory cytokines were downregulated in response to both VA and LA, and this downregulation was caused by a decrease in the activation of the NF-κB pathway and the translocation of p65, the pathway’s major component. Small nutraceutical molecules, VA and LA, showed not only inhibition of the pro-inflammatory cytokines, but also inhibition of the NF-κB activation, and reduced translocation of the p65 component. The present study may contribute to the therapeutic use of these molecules for various inflammatory diseases, which have in common an increased expression of pro-inflammatory cytokines and NF-κB-mediated inflammation.
- Published
- 2024
- Full Text
- View/download PDF
23. Dissecting Airborne Allergens.
- Author
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Torres-Borrego, Javier and Sánchez-Solís, Manuel
- Subjects
- *
ALLERGENS , *GENERAL practitioners , *ATOPIC dermatitis , *ALLERGIC rhinitis , *IMMUNOGLOBULIN E , *WHEEZE , *PEANUT allergy - Abstract
Asthma is a heterogeneous and very complex group of diseases, and includes different clinical phenotypes depending on symptoms, progression, exacerbation patterns, or responses to treatment, among other characteristics. The allergic phenotype is the most frequent, especially in pediatric asthma. It is characterized by sensitization (the production of specific IgEs) to allergens and frequent comorbidity with rhinitis as well as atopic dermatitis. Given the complexity of allergic asthma, knowledge of it must be approached from different points of view: clinical, histological, physiological, epidemiological, biochemical, and immunological, among others. Since partial approaches do not allow for the understanding of this complexity, it is necessary to have multidimensional knowledge that helps in performing the optimal management of each case, avoiding a "blind men and elephant parable" approach. Allergens are antigens that trigger the production of specific IgE antibodies in susceptible individuals, who present symptoms that will depend on the type and intensity of the allergenic load as well as the tissue where the interaction occurs. Airborne allergens cause their effects in the respiratory tract and eyes, and can be indoor or outdoor, perennial, or seasonal. Although allergens such as mites, pollens, or animal dander are generally considered single particles, it is important to note that they contain different molecules which could trigger distinct specific IgE molecules in different patients. General practitioners, pediatricians, and other physicians typically diagnose and treat asthma based on clinical and pulmonary function data in their daily practice. This nonsystematic and nonexhaustive revision aims to update other topics, especially those focused on airborne allergens, helping the diagnostic and therapeutic processes of allergic asthma and rhinitis. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
24. Mitochondrial dynamics modulate the allergic inflammation in a murine model of allergic rhinitis.
- Author
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Chen, Xu‐qing, Zhou, Long‐yun, Ma, Hua‐an, Wu, Ji‐yong, Liu, Shu‐fen, Wu, Yong‐jun, and Yan, Dao‐nan
- Subjects
- *
ALLERGIC rhinitis , *CONFOCAL fluorescence microscopy , *MITOCHONDRIA , *NASAL mucosa , *REACTIVE oxygen species - Abstract
Objective: Allergic rhinitis (AR) is a common allergic disorder, afflicting thousands of human beings. Aberrant mitochondrial dynamics are important pathological elements for various immune cell dysfunctions and allergic diseases. However, the connection between mitochondrial dynamics and AR remains poorly understood. This study aimed to determine whether mitochondrial dynamics influence the inflammatory response in AR. Methods: In the present study, we established a murine model of AR by sensitization with ovalbumin (OVA). Then, we investigated the mitochondrial morphology in mice with AR by transmission electron microscopy and confocal fluorescence microscopy, and evaluated the role of Mdivi‐1 (an inhibitor of mitochondrial fission) on allergic symptoms, inflammatory responses, allergic‐related signals, and reactive oxygen species formation. Results: There was a notable enhancement in mitochondrial fragmentation in the nasal mucosa of mice following OVA stimulation, whereas Mdivi‐1 prevented aberrant mitochondrial morphology. Indeed, Mdivi‐1 alleviated the rubbing and sneezing responses in OVA‐sensitized mice. Compared with vehicle‐treated ones, mice treated with Mdivi‐1 exhibited a reduction in interleukin (IL)‐4, IL‐5, and specific IgE levels in both serum and nasal lavage fluid, and shown an amelioration in inflammatory response of nasal mucosa. Meanwhile, Mdivi‐1 treatment was associated with a suppression in JAK2 and STAT6 activation and reactive oxygen species generation, which act as important signaling for allergic response. Conclusion: Our findings reveal mitochondrial dynamics modulate the allergic responses in AR. Mitochondrial dynamics may represent a promising target for the treatment of AR. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
25. Smokeless tobacco enhances allergic inflammation, aggravation of asthma and oxidative stress in asthmatic patients from Algeria.
- Author
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Khaldi, Taha, Boughemara, Karima, Khodja Hesnie, Yasmine, Amira, Aicha Beya, Messarah, Mahfoud, and Boumendjel, Amel
- Abstract
Purpose: Despite their effects on human health, the link between smokeless tobacco (ST) consumption and asthma severity in asthmatic patients is still unknown. Thus, the present study aims to complete the lack of information by investigating the aggravation of inflammation, aggravation of asthma, oxidative stress and cytotoxicity induced by ST in asthmatic patients. Methods: The study recruited 80 male volunteers residing in Annaba town, Algeria, divided into four groups by using a questionnaire, each group consisting of 20 male volunteers. Herein, biochemical parameters, hematological parameters, C-reactive protein (CRP), total IgE, interleukin-5 (IL-5), nitric oxide and oxidative stress were measured. Results: The obtained results showed that ST clearly enhanced lung inflammation and aggravation of asthma through total IgE, IL-5 and CRP increased production. In addition, ST was found to intensify oxidative stress via increased lipid peroxidation and decreased reduced glutathione (GSH) levels. Likewise, the biochemical and hematological parameters results showed that ST causes damage and inflammation to tissues. Conclusion: Therefore, our study reveals that ST obviously enhances allergic inflammation in patients suffering from asthma. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
26. The Role of Diet and Nutrition in Allergic Diseases.
- Author
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Zhang, Ping
- Abstract
Allergic diseases are a set of chronic inflammatory disorders of lung, skin, and nose epithelium characterized by aberrant IgE and Th2 cytokine-mediated immune responses to exposed allergens. The prevalence of allergic diseases, including asthma, allergic rhinitis, and atopic dermatitis, has increased dramatically worldwide in the past several decades. Evidence suggests that diet and nutrition play a key role in the development and severity of allergic diseases. Dietary components can differentially regulate allergic inflammation pathways through host and gut microbiota-derived metabolites, therefore influencing allergy outcomes in positive or negative ways. A broad range of nutrients and dietary components (vitamins A, D, and E, minerals Zn, Iron, and Se, dietary fiber, fatty acids, and phytochemicals) are found to be effective in the prevention or treatment of allergic diseases through the suppression of type 2 inflammation. This paper aims to review recent advances in the role of diet and nutrition in the etiology of allergies, nutritional regulation of allergic inflammation, and clinical findings about nutrient supplementation in treating allergic diseases. The current literature suggests the potential efficacy of plant-based diets in reducing allergic symptoms. Further clinical trials are warranted to examine the potential beneficial effects of plant-based diets and anti-allergic nutrients in the prevention and management of allergic diseases. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
27. "Outside-to-inside," "inside-to-outside," and "intrinsic" endogenous pathogenic mechanisms in atopic dermatitis: keratinocytes as the key functional cells involved in both permeability barrier dysfunction and immunological alterations.
- Author
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Yutaka Hatano and Elias, Peter M.
- Subjects
ATOPIC dermatitis ,PERMEABILITY ,KERATINOCYTES ,SINGLE molecules ,SKIN diseases - Abstract
Permeability barrier disruption has been shown to induce immunological alterations (i.e., an "outside-to-inside" pathogenic mechanism). Conversely, several inflammatory and immunological mechanisms reportedly interrupt permeability barrier homeostasis (i.e., an "inside-to-outside" pathogenic mechanism). It is now widely recognized that alterations of even a single molecule in keratinocytes can lead to not only permeability barrier dysfunction but also to immunological alterations. Such a simultaneous, bidirectional functional change by keratinocytes is herein named an "intrinsic" pathogenic mechanism. Molecules and/or pathways involved in this mechanism could be important not only as factors in disease pathogenesis but also as potential therapeutic targets for inflammatory cutaneous diseases, such as atopic dermatitis, psoriasis, and prurigo nodularis. Elevation of skin surface pH following permeability barrier abrogation comprises one of the key pathogenic phenomena of the "outside-to-inside" mechanism. Not only type 2 cytokines (e.g., IL-4, IL-13, IL-31) but also type 1 (e.g. IFN-γ), and type 3 (e.g., IL-17, IL-22) as well as several other inflammatory factors (e.g. histamine) can disrupt permeability barrier homeostasis and are all considered part of the "inside-tooutside" mechanism. Finally, examples of molecules relevant to the "intrinsic" pathogenic mechanism include keratin 1, filaggrin, and peroxisome proliferatoractivated receptor-α (PPARα). [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
28. Wheatgrass-and-Aronia-Mixed Extract Suppresses Immunoglobulin E-Mediated Allergic Reactions In Vitro and In Vivo.
- Author
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Lee, Ji-Hyun, Lim, Ji-Ye, Jeon, Yong-Deok, Yun, Dae-Ho, Lee, Young-Mi, and Kim, Dae-Ki
- Subjects
- *
ALLERGIES , *MAST cell disease , *MAST cells , *TRYPTASE , *IMMUNOGLOBULIN E , *EXTRACTS - Abstract
Mast cells are an important component of immune responses. Immunoglobulin (Ig) E-sensitized mast cells release substances within minutes of allergen exposure, triggering allergic responses. Until now, numerous pharmacological effects of wheatgrass and aronia have been verified, but the effects of wheatgrass and aronia (TAAR)-mixed extract on allergic reactions have not been identified. Therefore, the aim of this study was to demonstrate the anti-allergic effect of TAAR extract on mast cell activation and cutaneous anaphylaxis. In this study, we investigated the anti-allergic effects and related mechanisms of TAAR extract in IgE-activated mast cells in vitro. We also assessed the ameliorating effect of TAAR extract on IgE-mediated passive cutaneous anaphylaxis mice in vivo. The TAAR extract significantly reduced the expression of β-hexosaminidase, histamine, and pro-inflammatory cytokines, which are mediators related to mast cell degranulation, via the regulation of various signaling pathways. The TAAR extract also regulated oxidative-stress-related factors through the Nrf2 signaling pathway. Additionally, treatment of TAAR extract to the passive cutaneous anaphylaxis mouse model improved ear thickness and local ear pigmentation. Taken together, our results suggest that TAAR extract is a potential candidate natural product to treat overall IgE-mediated allergic inflammation and oxidative-stress-related diseases by suppressing mast cell activity. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
29. More airway smooth muscle in males versus females in a mouse model of asthma: A blessing in disguise?
- Author
-
Gill, Rebecka, Rojas‐Ruiz, Andrés, Boucher, Magali, Henry, Cyndi, and Bossé, Ynuk
- Subjects
- *
SMOOTH muscle , *RESPIRATORY mechanics , *LABORATORY mice , *HOUSE dust mites , *ANIMAL disease models , *COUGH - Abstract
New Findings: What is the central question of this study?The lung response to inhaled methacholine is reputed to be greater in male than in female mice. The underpinnings of this sex disparity are ill defined.What is the main finding and its importance?We demonstrated that male airways exhibit a greater content of airway smooth muscle than female airways. We also found that, although a more muscular airway tree in males might contribute to their greater responsiveness to inhaled methacholine than females, it might also curb the heterogeneity in small airway narrowing. Mouse models are helpful in unveiling the mechanisms underlying sex disparities in asthma. In comparison to their female counterparts, male mice are hyperresponsive to inhaled methacholine, a cardinal feature of asthma that contributes to its symptoms. The physiological details and the structural underpinnings of this hyperresponsiveness in males are currently unknown. Herein, BALB/c mice were exposed intranasally to either saline or house dust mite once daily for 10 consecutive days to induce experimental asthma. Twenty‐four hours after the last exposure, respiratory mechanics were measured at baseline and after a single dose of inhaled methacholine that was adjusted to trigger the same degree of bronchoconstriction in both sexes (it was twice as high in females). Bronchoalveolar lavages were then collected, and the lungs were processed for histology. House dust mite increased the number of inflammatory cells in bronchoalveolar lavages to the same extent in both sexes (asthma, P = 0.0005; sex, P = 0.96). The methacholine response was also markedly increased by asthma in both sexes (e.g., P = 0.0002 for asthma on the methacholine‐induced bronchoconstriction). However, for a well‐matched bronchoconstriction between sexes, the increase in hysteresivity, an indicator of airway narrowing heterogeneity, was attenuated in males for both control and asthmatic mice (sex, P = 0.002). The content of airway smooth muscle was not affected by asthma but was greater in males (asthma, P = 0.31; sex, P < 0.0001). These results provide further insights regarding an important sex disparity in mouse models of asthma. The increased amount of airway smooth muscle in males might contribute functionally to their greater methacholine response and, possibly, to their decreased propensity for airway narrowing heterogeneity. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
30. CD300a Regulates Mouse Macrophage Functionality in Allergic Inflammation.
- Author
-
Puzzovio, Pier Giorgio, Levy, Bruce D., and Levi-Schaffer, Francesca
- Subjects
- *
MACROPHAGES , *MAST cells , *MICE , *INFLAMMATION , *STAPHYLOCOCCUS aureus - Abstract
Background: CD300a is an inhibitory receptor (IR) expressed on several leukocytes, including mast cells (MCs) and macrophages (MΦ), important cells in allergic inflammation (AI). We have previously characterized CD300a role on MCs and in vivo in mouse models of allergy, in which the absence of CD300a resulted in increased inflammatory features and delayed resolution. However, the exact mechanism of this delayed resolution is unclear. Our hypothesis is that MΦ, important players in resolution, might be impaired when CD300a is absent. Objectives: The aim of the study was to investigate CD300a-dependent functionality of mouse MΦ. Method: MΦ were purified from the peritoneum of wild-type (WT) and CD300a−/− mice naïve and 48 h and 96 h after challenge with ovalbumin/alum. Phenotype switching was analyzed via specific M1-M2 inducers and markers. MΦ phagocytotic ability was assessed via Staphylococcus aureus pHrodo-conjugated bioparticles. The influence of MCs on MΦ was investigated by incubating WT MΦ with supernatants from non-activated and IgE-activated bone marrow-derived MCs (BMMCs) and analyzing functional responses. Results: Naïve CD300a−/− MΦ presented with increased sensitivity to activation when treated with LPS. Absence of CD300a results in increased Arg1 expression and increased IL-6 release when MΦ are purified from allergic peritonitis-induced mice. Similar results were obtained when CD300a−/− MΦ were purified 96 h after challenge. On the other hand, CD300a absence did not affect phagocytosis. WT MΦ incubated with supernatants of non-activated and IgE-activated BMMCs presented with increased iNOS expression and decreased Arg1 levels. Conclusions: The IR CD300a controls the activation state of MΦ, and its absence could augment the inflammatory state seen in CD300a−/− mice. Moreover, MCs can also influence MΦ phenotype switching. This may partially explain the delayed AI resolution seen in these mice. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
31. Downregulation of lncRNA CDKN2B-AS1 attenuates inflammatory response in mice with allergic rhinitis by regulating miR-98-5p/SOCS1 axis
- Author
-
Deng, Bangyu, zhao, Yunxia, and Liu, Jisheng
- Published
- 2024
- Full Text
- View/download PDF
32. Mitochondrial dynamics modulate the allergic inflammation in a murine model of allergic rhinitis
- Author
-
Xu‐qing Chen, Long‐yun Zhou, Hua‐an Ma, Ji‐yong Wu, Shu‐fen Liu, Yong‐jun Wu, and Dao‐nan Yan
- Subjects
allergic inflammation ,allergic rhinitis ,Mdivi‐1 ,mitochondrial dynamics ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Abstract Objective Allergic rhinitis (AR) is a common allergic disorder, afflicting thousands of human beings. Aberrant mitochondrial dynamics are important pathological elements for various immune cell dysfunctions and allergic diseases. However, the connection between mitochondrial dynamics and AR remains poorly understood. This study aimed to determine whether mitochondrial dynamics influence the inflammatory response in AR. Methods In the present study, we established a murine model of AR by sensitization with ovalbumin (OVA). Then, we investigated the mitochondrial morphology in mice with AR by transmission electron microscopy and confocal fluorescence microscopy, and evaluated the role of Mdivi‐1 (an inhibitor of mitochondrial fission) on allergic symptoms, inflammatory responses, allergic‐related signals, and reactive oxygen species formation. Results There was a notable enhancement in mitochondrial fragmentation in the nasal mucosa of mice following OVA stimulation, whereas Mdivi‐1 prevented aberrant mitochondrial morphology. Indeed, Mdivi‐1 alleviated the rubbing and sneezing responses in OVA‐sensitized mice. Compared with vehicle‐treated ones, mice treated with Mdivi‐1 exhibited a reduction in interleukin (IL)‐4, IL‐5, and specific IgE levels in both serum and nasal lavage fluid, and shown an amelioration in inflammatory response of nasal mucosa. Meanwhile, Mdivi‐1 treatment was associated with a suppression in JAK2 and STAT6 activation and reactive oxygen species generation, which act as important signaling for allergic response. Conclusion Our findings reveal mitochondrial dynamics modulate the allergic responses in AR. Mitochondrial dynamics may represent a promising target for the treatment of AR.
- Published
- 2023
- Full Text
- View/download PDF
33. IRF3 Activation in Mast Cells Promotes FcεRI-Mediated Allergic Inflammation.
- Author
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Choi, Young-Ae, Dhakal, Hima, Lee, Soyoung, Kim, Namkyung, Lee, Byungheon, Kwon, Taeg Kyu, Khang, Dongwoo, and Kim, Sang-Hyun
- Subjects
- *
MAST cells , *TRYPTASE , *KNOCKOUT mice , *INTERFERON regulatory factors - Abstract
(1) Background: This study aims to elucidate a novel non-transcriptional action of IRF3 in addition to its role as a transcription factor in mast cell activation and associated allergic inflammation; (2) Methods: For in vitro experiments, mouse bone-marrow-derived mast cells (mBMMCs) and a rat basophilic leukemia cell line (RBL-2H3) were used for investigating the underlying mechanism of IRF3 in mast-cell-mediated allergic inflammation. For in vivo experiments, wild-type and Irf3 knockout mice were used for evaluating IgE-mediated local and systemic anaphylaxis; (3) Results: Passive cutaneous anaphylaxis (PCA)-induced tissues showed highly increased IRF3 activity. In addition, the activation of IRF3 was observed in DNP-HSA-treated mast cells. Phosphorylated IRF3 by DNP-HSA was spatially co-localized with tryptase according to the mast cell activation process, and FcεRI-mediated signaling pathways directly regulated that activity. The alteration of IRF3 affected the production of granule contents in the mast cells and the anaphylaxis responses, including PCA- and ovalbumin-induced active systemic anaphylaxis. Furthermore, IRF3 influenced the post-translational processing of histidine decarboxylase (HDC), which is required for granule maturation; and (4) Conclusion: Through this study, we demonstrated the novel function of IRF3 as an important factor inducing mast cell activation and as an upstream molecule for HDC activity. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
34. CARD9 balances Aspergillus fumigatus‐induced anti‐fungal immunity and allergic inflammation.
- Author
-
Xu, Xia, Li, Fan, Huang, Ronghua, Wu, Siyuan, Huang, Zhiwen, and Duan, Jielin
- Subjects
- *
IMMUNITY , *PULMONARY eosinophilia , *ASPERGILLUS , *IMMUNOGLOBULIN E , *ALVEOLAR macrophages , *T helper cells , *PULMONARY aspergillosis , *ANTIALLERGIC agents - Abstract
Keywords: Allergic inflammation; Alveolar macrophages; Aspergillus fumigatus; CARD9 EN Allergic inflammation Alveolar macrophages Aspergillus fumigatus CARD9 659 663 5 06/09/23 20230601 NES 230601 Key message CARD9 enhances pro-inflammatory immune response, but inhibits pulmonary type 2 immunity after I A. fumigatus i exposure. Moreover, we sorted out alveolar macrophages from WT and CARD9 deficient mice infected with/without I A. fumigatus i , and further confirmed that RelB activation only occurred in CARD9 deficient alveolar macrophages. Simultaneously, we found that the expression of CD80, CD86 and MHC-II were comparable between WT and CARD9 deficient mice with/without I A. fumigatus i exposure, hinting that CARD9 did not affect the antigen presentation capacity of alveolar macrophages. CARD9 balances Aspergillus fumigatus-induced anti-fungal immunity and allergic inflammation. [Extracted from the article]
- Published
- 2023
- Full Text
- View/download PDF
35. Efficacy of Puressentiel Protective Nasal Spray in Allergic Rhinitis (EPPNS)
- Author
-
Institut National de la Santé Et de la Recherche Médicale, France
- Published
- 2021
36. Dynamics of the cellular composition of lymphoid nodules in the lungs of guinea pigs sensitized with ovalbumin
- Author
-
S.S. Popko
- Subjects
lymphoid nodule ,guinea pig ,lung ,allergic inflammation ,ovalbumin ,Medicine - Abstract
The article discusses the morphological aspects of the dynamics of the cellular composition of lymphoid nodules in the lungs of guinea pigs as a result of an experimental ovalbumin-induced allergic process. We studied the reactivity of immunocompetent cells of lymphoid formations of the lungs after three times subcutaneous sensitization and subsequent 8-day intranasal aeroallergization with ovalbumin in the early and late stage period of the allergic inflammatory process by microscopic, morphometric and statistical methods. By help of morphometric analysis we demonstrate the general regularity of reactivity of a local specific link of the pulmonary immune system to the action of an allergen, which consists in the elevation of the average amount of immune cells of lymphoid nodules of the lungs, starting from the 30th to the 44th day after the start of the experiment. The maximal coefficient of increase by 5.8 times was observed in counting plasma cells among all types of immunocompetent cells of lymphoid nodules in the lungs during the experiment. It has been statistically proven that the implementation of the ovalbumin-induced allergic inflammatory process in the lungs proceeds according to the humoral type and the duration of its course is not limited by the direct influence of the allergen, it also continues after the end of its action, which is a manifestation of changes in compensatory-adaptive processes in the pulmonary immune system with ovalbumin-induced allergic inflammation.
- Published
- 2022
- Full Text
- View/download PDF
37. Neuro-allergology: Mast cell–nerve cross-talk
- Author
-
Shota Toyoshima and Yoshimichi Okayama
- Subjects
Allergic inflammation ,Cross-talk ,Mast cell ,Nerve ,Neuropeptide ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Mast cells (MCs) are derived from hematopoietic stem cells in the bone marrow, and their maturation is regulated by the tissue environment, such as the skin, lung and gut, leading to host defense. Peripheral nerve fibers located in various tissues are involved in diverse physiological and pathological processes. Anatomical relationships between MCs and nerve fibers were reported to have been observed in various organs. Moreover, MCs are positive for a large number of receptors for classical neurotransmitters (e.g., acetylcholine and corticotropin-releasing hormone) and neuropeptides (e.g., substance P, calcitonin gene-related peptides and hemokinin), and MC's functions are regulated by those nerve-derived factors. Also, histamine and proteases produced and released by MCs modulate nerve fiber functions. This functional cross-talk between MCs and nerve fibers can play physiological and pathological roles. MCs are key effector cells of allergic inflammation, such as atopic dermatitis, airway inflammation and food allergy. Here, we summarize and discuss the molecular mechanisms underlying the functional and anatomical cross-talk between MCs and nerve fibers in allergic inflamed tissues.
- Published
- 2022
- Full Text
- View/download PDF
38. Role of L-selectin and ICAM-1 adhesion molecules in children with asthma
- Author
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P. V. Berezhansky, N. S. Tataurschikova, T. G. Fedoskova, O. I. Letyaeva, A. S. Rusanova, I. N. Grigorieva, A. A. Kameleva, A. E. Dobrenkaya, and A. I. Vasilkova
- Subjects
asthma ,adhesion molecules ,virus ,allergic inflammation ,asthma exacerbation ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Аsthma is among the commonest chronic bronchopulmonary diseases in childhood, being a serious medical, social and economic problem. Asthma represents a multifactorial chronic inflammatory disease characterized by activation of T-mediated factors, including adhesion molecules in bronchial mucosa, as well as minimal persistent inflammation which is characterized by a long-term inflammatory process (despite complete absence of clinical manifestations) in the patients with allergic disorders accompanied by increased expression of ICAM-1 (type 1 intercellular adhesion molecule) and CD62L (L-selectin) in the bloodstream.Lymphocyte and eosinophil counts in allergic inflammation show direct dependence on ICAM-1 contents, an intercellular adhesion molecule that provides transmigration of eosinophils and leukocytes through the endothelial barrier. Increased amount of ICAM-1 directly depends on excessive production of various reactive oxygen species in bronchial asthma. In turn, ICAM-1 induces changes in the cellular cytoskeleton which play a significant role in pathogenesis of asthma. It has been noted that ICAM-1 and CD62L molecules are those factors that exert changes at the microrheological level, including respiratory pathology of allergic nature. Increased amounts of vascular adhesion molecules in respiratory tract It has been proven are proven to be an important component of pathogenesis in bronchial asthma.Maximal expression of vascular cell adhesion molecule 1 (VCAM-1) and ICAM-1 in the persons prone to allergic diseases may occur after undetermined time period, and it immediately causes pronounced degranulation of eosinophils in respiratory tract and capillary bed. Viral infection is also an important trigger for the asthma exacerbation. Epithelial expression of intercellular adhesion molecule ICAM-1, a cellular receptor for the most rhinoviruses, is increased after the rhinovirus infection itself. Both eosinophils and neutrophils contribute to the development of severe asthma, or exacerbation of asthma. ICAM-1 is a cellular receptor for rhinoviruses. Adhesion of eosinophils to ICAM-1 promotes functional activation of eosinophils. Therefore, adhesion of eosinophils to epithelial cells via ICAM-1 may activate this population during exacerbation in bronchial asthma.Changes in the immunohemorheology system in children with bronchial asthma represent the starting point of disorders at either hemostatic pathways, with a trend for increased adhesiveness and hypercoagulability, thus activating entire cascade of immunometabolic disorders and initiate clinical development of asthma. Exacerbation of asthma is characterized by the distinct expression pattern of the ICAM-1 adhesion factor, depending on the agent which promotes the airway obstruction. In the patients with asthma, depending on severity of exacerbation, there are pronounced changes in the levels of adhesion molecules. A pronounced increase in ICAM-1 at the time of bronchial obstruction is caused by the both causal allergen and infectious agent. However, more pronounced increase occurs during pollination, as well as slight elevation is observed in the course of obstruction caused by an infectious agent.
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- 2022
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39. Fatty-Acid-Based Membrane Lipidome Profile of Peanut Allergy Patients: An Exploratory Study of a Lifelong Health Condition.
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Del Duca, Elisabetta, Sansone, Anna, Sgrulletti, Mayla, Di Nolfo, Federica, Chini, Loredana, Ferreri, Carla, and Moschese, Viviana
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- *
PEANUT allergy , *IMMUNOGLOBULIN E , *UNSATURATED fatty acids , *FATTY acid methyl esters , *BODY mass index , *EICOSAPENTAENOIC acid , *DOCOSAHEXAENOIC acid , *ARACHIDONIC acid - Abstract
Peanut allergy is a lifelong, increasingly prevalent, and potentially life-threatening disease burdening families and communities. Dietary, particularly polyunsaturated fatty acids (PUFAs), intakes can exert positive effects on immune and inflammatory responses, and the red blood cell (RBC) membrane lipidome contains stabilized metabolic and nutritional information connected with such responses. The fatty-acid-based membrane lipidome profile has been exploratorily evaluated in a small cohort of patients (eight males and one female, age range 4.1–21.7 years old, body mass index BMI < 25) with angioedema and/or anaphylaxis after peanut ingestion. This analysis was performed according to an ISO 17025 certified robotic protocol, isolating mature RBCs, extracting membrane lipids, and transforming them to fatty acid methyl esters for gas chromatography recognition and quantification. Comparison with a group of age- and BMI-matched healthy individuals and with benchmark interval values of a healthy population evidenced significant differences, such as higher levels of ω-6 (arachidonic acid), lower values of ω-3 eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), together with an increased ω-6/ω-3 ratio in allergic patients. A significant inverse correlation was also found between specific immunoglobulin E (IgE) levels and ω-6 di-homo-gamma-linolenic acid (DGLA) and total PUFAs. Results of this preliminary study encourage screenings in larger cohorts, also in view of precision nutrition and nutraceuticals strategies, and stimulate interest to expand basic and applied research for unveiling molecular mechanisms that are still missing and individuating treatments in chronic allergic disorders. [ABSTRACT FROM AUTHOR]
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- 2023
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40. Experimental allergic airway inflammation impacts gut homeostasis in mice
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Carolina Martins Nascimento, Mateus Campos Casaro, Evelyn Roxana Perez, Willian Rodrigues Ribeiro, Marcia Pinto Alves Mayer, Karin Hitomi Ishikawa, Adriana Lino-dos-Santos-Franco, Joice Naiara Bertaglia Pereira, and Caroline Marcantonio Ferreira
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Asthma ,Allergic inflammation ,Lung ,Gut and mucosa ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Background: /Aims: Epidemiological data show that there is an important relationship between respiratory and intestinal diseases. To improve our understanding on the interconnectedness between the lung and intestinal mucosa and the overlap between respiratory and intestinal diseases, our aim was to investigate the influence of ovalbumin (OVA)-induced allergic airway inflammation on gut homeostasis. Methods: A/J mice were sensitized and challenged with OVA. The animals were euthanized 24 h after the last challenge, lung inflammation was determined by evaluating cells in Bronchoalveolar lavage fluid, serum anti-OVA IgG titers and colon morphology, inflammation and integrity of the intestinal mucosa were investigated. IL-4 and IL-13 levels and myeloperoxidase activity were determined in the colon samples. The expression of genes involved in inflammation and mucin production at the gut mucosa was also evaluated. Results: OVA challenge resulted not only in lung inflammation but also in macroscopic alterations in the gut such as colon shortening, increased myeloperoxidase activity and loss of integrity in the colonic mucosal. Neutral mucin intensity was lower in the OVA group, which was followed by down-regulation of transcription of ATOH1 and up-regulation of TJP1 and MUC2. In addition, the OVA group had higher levels of IL-13 and IL-4 in the colon. Ova-specific IgG1 and OVA-specific IgG2a titers were higher in the serum of the OVA group than in controls. Conclusions: Our data using the OVA experimental model suggested that challenges in the respiratory system may result not only in allergic airway inflammation but also in the loss of gut homeostasis.
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- 2023
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41. Changes in Adaptive Immune Responses and Effector Cell Responses Upon Nasal Allergen Exposure - a Pilot Study
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Ass.Prof.Dr.med. Josef Toth, Associate Professor, Doctor of Medicine
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- 2019
42. Mast cells contribute to the resolution of allergic inflammation by releasing resolvin D1
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Pier Giorgio Puzzovio, Hadas Pahima, Tresa George, David Mankuta, Ron Eliashar, Ekaterini Tiligada, Bruce D. Levy, and Francesca Levi-Schaffer
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Allergic inflammation ,Allergic peritonitis ,Eosinophils ,Mast cells ,Resolution ,Resolvin D1 ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Background: Mast cells are initiators and main effectors of allergic inflammation, together with eosinophils, with whom they can interact in a physical and soluble cross-talk with marked pro-inflammatory features, the Allergic Effector Unit. The pro-resolution role of mast cells, alone or in co-culture with eosinophils, has not been characterized yet. Objectives: We aimed to investigate select pro-resolution pathways in mast cells in vitro and in vivo in allergic inflammation. Methods: In vitro, we employed human and murine mast cells and analyzed release of resolvin D1 and expression of 15-lipoxygenase after IgE-mediated activation. We performed co-culture of IgE-activated mast cells with peripheral blood eosinophils and investigated 15-lipoxygenase expression and Resolvin D1 release. In vivo, we performed Ovalbumin/Alum and Ovalbumin/S. aureus enterotoxin B allergic peritonitis model in Wild Type mice following a MC “overshoot” protocol. Results: We found that IgE-activated mast cells release significant amounts of resolvin D1 30 min after activation, while 15-lipoxygenase expression remained unchanged. Resolvin D1 release was found to be decreased in IgE-activated mast cells co-cultured with peripheral blood eosinophils for 30 min In vivo, mast cell-overshoot mice exhibited a trend of reduced inflammation, together with increased peritoneal resolvin D1 release. Conclusions: Mast cells can actively contribute to resolution of allergic inflammation by releasing resolvin D1.
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- 2023
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43. Supplementation of syringic acid-rich Phrynium pubinerve leaves imparts protection against allergic inflammatory responses by downregulating iNOS, COX-2, and NF-κB expressions
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Md Arman Islam, Md Samiul Huq Atanu, Md Afjalus Siraj, Rabindra Nath Acharyya, Khondoker Shahin Ahmed, Shrabanti Dev, Shaikh Jamal Uddin, and Asish Kumar Das
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Phrynium pubinerve ,Allergic inflammation ,Syringic acid ,iNOS ,COX-2 ,NF-κB ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Background: The present study was designed to characterize the role of ethanolic leaf extract of Phrynium pubinerve Blume (EPP) supplement in attenuating allergic inflammation, encouraged by the presence of syringic acid in it, as this phenolic acid is reportedly promising in suppressing serum immunoglobulin E (IgE) and inflammatory cytokine levels. Materials and methods: HPLC-DAD dereplication analysis was performed to determine the presence of the vital polyphenolic metabolites. The efficacy of EPP against lipopolysaccharide (LPS)-induced inflammation in RAW 264.7 cells was evaluated by measuring its inhibitory effects on NO and ROS/RNS production. The expressions of major inflammation-associated molecules (iNOS, COX-2, NF-κB, IL-6, and TNF-α) in RAW 264.7 cells were assessed through Western blot. Physiological and behavioral changes, BMI, and different biochemical parameters in mice blood serum were investigated in the toxicological assays. Formaldehyde-induced paw edema test in mice was conducted using established animal model. TDI-induced allergic model in mice was carried out to determine different allergy-like symptoms, and differential white blood cell (WBC) counts in blood and bronchoalveolar lavage (BAL) fluid. The intermolecular interaction analysis of the identified major metabolite of EPP with H1R and iNOS was studied by molecular docking. Results: HPLC-DAD analysis showed the presence of syringic acid (89.19 mg/100 g EPP) and a few other compounds. LPS-induced NO generation was reduced by EPP in a concentration-dependent manner, showing IC50 of 28.20 ± 0.27 μg/mL. EPP exhibited a similar inhibitory effect on ROS/RNS production with IC50 of 29.47 ± 2.19 μg/mL. Western blotting revealed that EPP significantly downregulated the expressions of iNOS, COX-2, NF-κB, IL-6, and TNF-α in RAW 264.7 cells when challenged with LPS. The toxicological assays confirmed the dosage and organ-specific safety of EPP. In the formaldehyde-induced paw edema test, EPP caused a 66.41% reduction in mice paw volume at 500 mg/kg dose. It ameliorated TDI-induced allergy-like symptoms and decreased different inflammatory WBCs in mice's blood and BAL fluid in a dose-dependent manner. Finally, syringic acid demonstrated mentionable intermolecular binding affinity towards H1R (−6.6 Kcal/moL) and iNOS (−6.7 Kcal/moL). Conclusions: Collectively, considerable scientific reasoning was obtained in favor of the suppressive potential of EPP against allergic inflammatory responses that are proposed to be exerted via the downregulation of iNOS, COX-2, and NF-κB expressions, H1R antagonism and suppression of cytokines, such as IL-6, and TNF-α.
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- 2023
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44. The Crosstalk between FcεRI and Sphingosine Signaling in Allergic Inflammation.
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Jo, Hyein, Shim, Kyeonghee, and Jeoung, Dooil
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- *
SPHINGOSINE , *ALLERGIES , *ANTIALLERGIC agents , *SKIN diseases , *MAST cells - Abstract
Sphingolipid molecules have recently attracted attention as signaling molecules in allergic inflammation diseases. Sphingosine-1-phosphate (S1P) is synthesized by two isoforms of sphingosine kinases (SPHK 1 and SPHK2) and is known to be involved in various cellular processes. S1P levels reportedly increase in allergic inflammatory diseases, such as asthma and anaphylaxis. FcεRI signaling is necessary for allergic inflammation as it can activate the SPHKs and increase the S1P level; once S1P is secreted, it can bind to the S1P receptors (S1PRs). The role of S1P signaling in various allergic diseases is discussed. Increased levels of S1P are positively associated with asthma and anaphylaxis. S1P can either induce or suppress allergic skin diseases in a context-dependent manner. The crosstalk between FcεRI and S1P/SPHK/S1PRs is discussed. The roles of the microRNAs that regulate the expression of the components of S1P signaling in allergic inflammatory diseases are also discussed. Various reports suggest the role of S1P in FcεRI-mediated mast cell (MC) activation. Thus, S1P/SPHK/S1PRs signaling can be the target for developing anti-allergy drugs. [ABSTRACT FROM AUTHOR]
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- 2022
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45. A pathogenic integrated view explaining the different endotypes of asthma and allergic disorders.
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Maggi, Enrico, Parronchi, Paola, Azzarone, Bruno Giuseppe, and Moretta, Lorenzo
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- *
INNATE lymphoid cells , *ASTHMA , *ALLERGIES - Abstract
The inflammation of allergic diseases is characterized by a complex interaction between type 2 and type 3 immune responses, explaining clinical symptoms and histopathological patterns. Airborne stimuli activate the mucosal epithelium to release a number of molecules impacting the activity of resident immune and environmental cells. Signals from the mucosal barrier, regulatory cells, and the inflamed tissue are crucial conditions able to modify innate and adaptive effector cells providing the selective homing of eosinophils or neutrophils. The high plasticity of resident T‐ and innate lymphoid cells responding to external signals is the prerequisite to explain the multiplicity of endotypes of allergic diseases. This notion paved the way for the huge use of specific biologic drugs interfering with pathogenic mechanisms of inflammation. Based on the response of the epithelial barrier, the activity of resident regulatory cells, and functions of structural non‐lymphoid environmental cells, this review proposes some immunopathogenic scenarios characterizing the principal endotypes which can be associated with a precise phenotype of asthma. Recent literature indicates that similar concepts can also be applied to the inflammation of other non‐respiratory allergic disorders. The next challenges will consist in defining specific biomarker(s) of each endotype allowing for a quick diagnosis and the most effective personalized therapy. [ABSTRACT FROM AUTHOR]
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- 2022
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46. The impact of type 2 immunity and allergic diseases in atherosclerosis.
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Fernández‐Gallego, Nieves, Castillo‐González, Raquel, Méndez‐Barbero, Nerea, López‐Sanz, Celia, Obeso, David, Villaseñor, Alma, Escribese, María M., López‐Melgar, Beatriz, Salamanca, Jorge, Benedicto‐Buendía, Amparo, Jiménez‐Borreguero, Luis Jesús, Ibañez, Borja, Sastre, Joaquín, Belver, María Teresa, Vega, Francisco, Blanco, Carlos, Barber, Domingo, Sánchez‐Madrid, Francisco, de la Fuente, Hortensia, and Martín, Pilar
- Subjects
- *
ALLERGIES , *TH2 cells , *ATHEROSCLEROSIS , *ALLERGIC rhinitis , *ATOPIC dermatitis , *ENDOTHELIUM diseases , *ATOPY - Abstract
Allergic diseases are allergen‐induced immunological disorders characterized by the development of type 2 immunity and IgE responses. The prevalence of allergic diseases has been on the rise alike cardiovascular disease (CVD), which affects arteries of different organs such as the heart, the kidney and the brain. The underlying cause of CVD is often atherosclerosis, a disease distinguished by endothelial dysfunction, fibrofatty material accumulation in the intima of the artery wall, smooth muscle cell proliferation, and Th1 inflammation. The opposed T‐cell identity of allergy and atherosclerosis implies an atheroprotective role for Th2 cells by counteracting Th1 responses. Yet, the clinical association between allergic disease and CVD argues against it. Within, we review different phases of allergic pathology, basic immunological mechanisms of atherosclerosis and the clinical association between allergic diseases (particularly asthma, atopic dermatitis, allergic rhinitis and food allergy) and CVD. Then, we discuss putative atherogenic mechanisms of type 2 immunity and allergic inflammation including acute allergic reactions (IgE, IgG1, mast cells, macrophages and allergic mediators such as vasoactive components, growth factors and those derived from the complement, contact and coagulation systems) and late phase inflammation (Th2 cells, eosinophils, type 2 innate‐like lymphoid cells, alarmins, IL‐4, IL‐5, IL‐9, IL‐13 and IL‐17). [ABSTRACT FROM AUTHOR]
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- 2022
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47. Prostacyclin Regulation of Allergic Inflammation.
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Patel, Kunj and Peebles Jr., R. Stokes
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PROSTACYCLIN ,TH2 cells ,T cells ,PULMONARY hypertension ,DENDRITIC cells - Abstract
Prostacyclin is a metabolic product of the cyclooxygenase pathway that is constitutively expressed and can be induced during inflammatory conditions. While prostacyclin and its analogs have historically been considered effective vasodilators and used in treating pulmonary hypertension, prostacyclin has demonstrated potent anti-inflammatory effects in animal models of allergic airway inflammation. In vitro studies reveal that prostacyclin directly inhibits type 2 cytokine production from CD4+ Th2 cells and ILC2 and reduces the ability of dendritic cells to generate Th2 cytokine production from CD4+ T cells in an antigen-specific manner. Thus, there is strong evidence that prostacyclin may be an additional therapeutic target for treating allergic inflammation and asthma in human subjects. [ABSTRACT FROM AUTHOR]
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- 2022
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48. Inhibitory Effects of Grewia tomentosa Juss. on IgE-Mediated Allergic Reaction and DNCB-Induced Atopic Dermatitis.
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Lee, Hwa Pyoung, Choi, Wooram, Kwon, Ki Woong, You, Long, Rahmawati, Laily, Luong, Van Dung, Kim, Wonhee, Lee, Byoung-Hee, Lee, Sarah, Kim, Ji Hye, and Cho, Jae Youl
- Subjects
ATOPIC dermatitis ,ALLERGIES ,MAST cells ,THERAPEUTICS ,IMMUNOGLOBULIN E ,HERBAL medicine - Abstract
Grewia tomentosa Juss. is a deciduous shrub that mainly grows in Asia. Despite studies of other Grewia species for treatment of various diseases, Grewia tomentosa Juss. has not been studied as a medicinal herb. This study evaluates the anti-allergic and anti-topic dermatitis activity of Grewia tomentosa Juss. ethanol extract (Gt-EE). The results show that Gt-EE suppressed IgE–antigen-induced β-hexosaminidase release. The mRNA expression of IL-1β, IL-4, IL-5, IL-6, IL-13, TNF-α, MCP-1, and TSLP, which are involved in allergic responses, was inhibited by Gt-EE in IgE-stimulated RBL-2H3 cells. In addition, the phosphorylation of Syk, PLCγ1, PKCδ, PI3K, AKT, NF-κB p65, NF-κB p50, p38, JNK, and ERK1/2 was decreased by Gt-EE in these cells. Gt-EE also showed anti-inflammatory effects in in vivo mouse models. In passive cutaneous anaphylaxis (PCA), a commonly used mouse model, Gt-EE decreased the allergic response, infiltration of mast cells, and mRNA level of IL-4. Furthermore, Gt-EE ameliorated symptoms of DNCB-induced atopic dermatitis (AD). In DNCB-induced AD, Gt-EE suppressed the increase in mast cells, serum IgE level, expression of allergic mediators (IL-1β, IL-4, IL-5, IL-6, TNF-α), and phosphorylation of proteins (IκBα, NF-κB p65, NF-κB p50, p38, JNK, and ERK1/2) implicated in allergic reactions [ABSTRACT FROM AUTHOR]
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- 2022
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49. Dissecting Airborne Allergens
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Javier Torres-Borrego and Manuel Sánchez-Solís
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airborne allergens ,allergic inflammation ,asthma ,allergic rhinoconjunctivitis ,sensitization ,allergy ,Medicine - Abstract
Asthma is a heterogeneous and very complex group of diseases, and includes different clinical phenotypes depending on symptoms, progression, exacerbation patterns, or responses to treatment, among other characteristics. The allergic phenotype is the most frequent, especially in pediatric asthma. It is characterized by sensitization (the production of specific IgEs) to allergens and frequent comorbidity with rhinitis as well as atopic dermatitis. Given the complexity of allergic asthma, knowledge of it must be approached from different points of view: clinical, histological, physiological, epidemiological, biochemical, and immunological, among others. Since partial approaches do not allow for the understanding of this complexity, it is necessary to have multidimensional knowledge that helps in performing the optimal management of each case, avoiding a “blind men and elephant parable” approach. Allergens are antigens that trigger the production of specific IgE antibodies in susceptible individuals, who present symptoms that will depend on the type and intensity of the allergenic load as well as the tissue where the interaction occurs. Airborne allergens cause their effects in the respiratory tract and eyes, and can be indoor or outdoor, perennial, or seasonal. Although allergens such as mites, pollens, or animal dander are generally considered single particles, it is important to note that they contain different molecules which could trigger distinct specific IgE molecules in different patients. General practitioners, pediatricians, and other physicians typically diagnose and treat asthma based on clinical and pulmonary function data in their daily practice. This nonsystematic and nonexhaustive revision aims to update other topics, especially those focused on airborne allergens, helping the diagnostic and therapeutic processes of allergic asthma and rhinitis.
- Published
- 2023
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50. The Role of Diet and Nutrition in Allergic Diseases
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Ping Zhang
- Subjects
allergy ,allergic inflammation ,asthma ,allergic rhinitis ,atopic dermatitis ,dietary lipids ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Allergic diseases are a set of chronic inflammatory disorders of lung, skin, and nose epithelium characterized by aberrant IgE and Th2 cytokine-mediated immune responses to exposed allergens. The prevalence of allergic diseases, including asthma, allergic rhinitis, and atopic dermatitis, has increased dramatically worldwide in the past several decades. Evidence suggests that diet and nutrition play a key role in the development and severity of allergic diseases. Dietary components can differentially regulate allergic inflammation pathways through host and gut microbiota-derived metabolites, therefore influencing allergy outcomes in positive or negative ways. A broad range of nutrients and dietary components (vitamins A, D, and E, minerals Zn, Iron, and Se, dietary fiber, fatty acids, and phytochemicals) are found to be effective in the prevention or treatment of allergic diseases through the suppression of type 2 inflammation. This paper aims to review recent advances in the role of diet and nutrition in the etiology of allergies, nutritional regulation of allergic inflammation, and clinical findings about nutrient supplementation in treating allergic diseases. The current literature suggests the potential efficacy of plant-based diets in reducing allergic symptoms. Further clinical trials are warranted to examine the potential beneficial effects of plant-based diets and anti-allergic nutrients in the prevention and management of allergic diseases.
- Published
- 2023
- Full Text
- View/download PDF
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