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322 results on '"Allouche, M."'

9. Resveratrol Reverses Endothelial Colony-Forming Cell Dysfunction in Adulthood in a Rat Model of Intrauterine Growth Restriction

Author

Guillot, E., Lemay, A., Allouche, M., Vitorino Silva, S., Coppola, H., Sabatier, F., Dignat-George, F., Sarre, A., Peyter, A.C., Simoncini, S., and Yzydorczyk, C.

Subjects
Humans, Male, Female, Rats, Animals, Fetal Growth Retardation/metabolism, Resveratrol/pharmacology, Resveratrol/metabolism, Fluorescent Dyes/metabolism, Endothelial Cells/metabolism, Cardiovascular Diseases/metabolism, Cell Proliferation, Cells, Cultured, developmental programming, endothelial colony-forming cells, intrauterine growth restriction, oxidative stress, resveratrol, stress-induced premature senescence
Abstract

Individuals born after intrauterine growth restriction (IUGR) are at risk of developing cardiovascular diseases (CVDs). Endothelial dysfunction plays a role in the pathogenesis of CVDs; and endothelial colony-forming cells (ECFCs) have been identified as key factors in endothelial repair. In a rat model of IUGR induced by a maternal low-protein diet, we observed an altered functionality of ECFCs in 6-month-old males, which was associated with arterial hypertension related to oxidative stress and stress-induced premature senescence (SIPS). Resveratrol (R), a polyphenol compound, was found to improve cardiovascular function. In this study, we investigated whether resveratrol could reverse ECFC dysfunctions in the IUGR group. ECFCs were isolated from IUGR and control (CTRL) males and were treated with R (1 μM) or dimethylsulfoxide (DMSO) for 48 h. In the IUGR-ECFCs, R increased proliferation (5'-bromo-2'-deoxyuridine (BrdU) incorporation, p < 0.001) and improved capillary-like outgrowth sprout formation (in Matrigel), nitric oxide (NO) production (fluorescent dye, p < 0.01), and endothelial nitric oxide synthase (eNOS) expression (immunofluorescence, p < 0.001). In addition, R decreased oxidative stress with reduced superoxide anion production (fluorescent dye, p < 0.001); increased Cu/Zn superoxide dismutase expression (Western blot, p < 0.05); and reversed SIPS with decreased beta-galactosidase activity (p < 0.001), and decreased p16 ink4a (p < 0.05) and increased Sirtuin-1 (p < 0.05) expressions (Western blot). No effects of R were observed in the CTRL-ECFCs. These results suggest that R reverses long-term ECFC dysfunctions related to IUGR.

Published
2023

20. Traumatisme sur rein pathologique : y a-t-il une sur-morbidité ?

Author

Maatougui, J., primary, Saadi, A., additional, Chakroun, M., additional, Bellali, M., additional, Allouche, M., additional, Jrad, M., additional, Derouiche, A., additional, Bouzouita, A., additional, Ben Slama, M., additional, Hamdoun, M., additional, Ayed, H., additional, and Chebil, M., additional

Published
2021
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23. Late Pleistocene submarine terraces in the Eastern Mediterranean, central Lebanon, Byblos: Revealing their formation time frame through modeling

Author

Georgiou, N., Geraga, M., Francis-Allouche, M., Christodoulou, D., Stocchi, P., Fakiris, E., Dimas, X., Zoura, D., Iatrou, M., Papatheodorou, G., Georgiou, N., Geraga, M., Francis-Allouche, M., Christodoulou, D., Stocchi, P., Fakiris, E., Dimas, X., Zoura, D., Iatrou, M., and Papatheodorou, G.

Abstract

Important evidence related to sea-level fluctuation, human evolution and dispersal that took place onshore during the Late Pleistocene and Holocene eras, are currently found underwater due to the sea-level rise. In this study, we present submarine relative paleo sea-level indicators found offshore the Lebanese coastline, at large from the prominent ancient city of Byblos. Two different submarine erosional terrace sequences were identified at depths ranging from −40 to −25m (“distant” sequence) and −11 to 0m (“nearshore” sequence) below present sea level, by using a combination of high-resolution geophysical means and terrain data analysis techniques. In the absence of datings, a model that uses relative sea-level curves for different uplift rates and examines the terraces' formation for various cliff retreat rates (derived from literature and on-field GPS measurements) was built. This model indicates the most possible time frames of the submarine terraces’ (STs) formation. The “nearshore” terrace sequence is suggested to have originally been formed during Marine Isotope Stage 5a (MIS5a) sea-level highstand, while it was possibly re-flattened during the first MIS1 sea-level slowdown (8–6 ka BP). The “distant” sequence formation is placed between the MIS4 to MIS3 transition (∼62–50 ka BP), and during MIS3 sea-level highstands within 45–35 ka BP. It was also found that a long-term uplift rate of 0.28–0.37 mm/a and cliff retreat rate of 0.03–0.09 m/a best fit our data and existing onshore relative sea-level indicators. The formation of the STs at this time was further supported by chronologically intercurrent uplifting events that facilitated their formation and preservation. Finally, our model supports recent ice-sheet reconstructions related to higher MIS3 values since sea-level curves that were tested for this scenario (eustatically reaching up to −37/−38m depth), proved more successful in the formation of the “distant” sequence.

Published
2021

34. Corrigendum to 'Development of a combined solver to model transport and chemical reactions in catalytic wall-flow filters' [Chem. Eng. Res. Des. 117 (2016) 681–687] (S0263876216304324) (10.1016/j.cherd.2016.11.014)

Author

Allouche M. H., Enjalbert R., Alberini F., Ariane M., Liu L., Wilkinson S. K., Alexiadis A., Allouche M.H., Enjalbert R., Alberini F., Ariane M., Liu L., Wilkinson S.K., and Alexiadis A.

Subjects
CFD Reactive flow Heat and mass transfer Catalytic converter Diesel particulate filter
Abstract

The authors regret that two of the authors, Li Liu and Sam K. Wilkinson, were omitted in error during the publication of this article. Hence the full list of authors is now shown in this corrigendum. The authors would like to apologise for any inconvenience caused.

Published
2017

44. Late cardiomyopathy in childhood acute myeloid leukemia survivors: a study from the L.E.A. program

Author

Barlogis, V., primary, Auquier, P., additional, Bertrand, Y., additional, Chastagner, P., additional, Plantaz, D., additional, Poiree, M., additional, Kanold, J., additional, Berbis, J., additional, Oudin, C., additional, Vercasson, C., additional, Allouche, M., additional, Tabone, M.-D., additional, Thouvenin-Doulet, S., additional, Saumet, L., additional, Chambost, H., additional, Baruchel, A., additional, Leverger, G., additional, and Michel, G., additional

Published
2015
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48. Daunorubicin-induced internucleosomal DNA fragmentation in acute myeloid cell lines

Author

Quillet-Mary, A., Mansat, V., Duchayne, E., Come, M. G., Allouche, M., Jean-Denis BAILLY, Bordier, C., Laurent, G., Laboratoire de Pharmacologie et de Toxicologie Fondamentales (LPTF), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), CHU Dijon, Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), QUILLET-MARY, Anne, and Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.MHEP.HEM] Life Sciences [q-bio]/Human health and pathology/Hematology, Pyrrolidines, Apoptosis, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Antioxidants, DNA Nucleotidylexotransferase, Thiocarbamates, Methionine Sulfoximine, Tumor Cells, Cultured, Humans, ATP Binding Cassette Transporter, Subfamily B, Member 1, Buthionine Sulfoximine, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, health care economics and organizations, Antibiotics, Antineoplastic, Daunorubicin, Cell Differentiation, [SDV.MHEP.HEM]Life Sciences [q-bio]/Human health and pathology/Hematology, DNA, Neoplasm, Free Radical Scavengers, humanities, Acetylcysteine, Nucleosomes, Leukemia, Myeloid, Acute, Verapamil, DNA Damage
Abstract

International audience; The study was designed to evaluate the implication of apoptosis in myeloid leukemic cell death induced by daunorubicin (DNR) and to identify the possible factors which may influence this process. DNR-induced apoptosis was characterized by morphology and DNA fragmentation in six leukemic myeloid cell lines which expressed different differentiation phenotypes. In phenotypically mature HL-60 and U937 cells, DNR induced typical apoptosis with characteristic morphological changes and intense internucleosomal DNA fragmentation within a narrow concentration range (0.5-2 microM). When these cells were treated with higher doses of DNR, large DNA fragments (100 kbp), but not internucleosomal fragments, were identified. DNR-induced DNA fragmentation in HL-60 and U937 was inhibited by antioxidants such as N-acetylcysteine (N-ac) or pyrrolidine-dithiocarbamate (PDTC). In the phenotypically immature KG1a, KG1, HEL and ML1 cell lines DNR induced no characteristic apoptotic morphological features as well as very low levels of internucleosomal DNA fragmentation, whereas large DNA fragments (200 kbp) were observed in KG1a treated with 7 microM DNR. Since the latter expressed P-glycoprotein (P-gp), the role of P-gp in the lack of apoptotic response to DNR was investigated. One P-gp inhibitor (verapamil) slightly improved DNR-induced DNA fragmentation in KG1a cells whereas the combination of verapamil and buthionine-sulfoximine (BSO), which depletes glutathion store, further increased internucleosomal DNA fragmentation. In conclusion, DNR induced internucleosomal DNA fragmentation in some but not all AML cells; the magnitude of this process being influenced by both intracellular drug concentration and oxidative balance.

Published
1996

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