Search

Background: Asprosin is correlated to many pathologic of glucose dysregulation, insulin resistance, ß-cell dysfunction, serum lipids, and adiposity. Objective investigating the role of the asprosin hormone and its relationship to insulin resistance in newly diagnosed metabolic syndrome and clinical parameters. Methods: The study included measurement of asprosin hormone, insulin, insulin resistance and some biochemical variable levels in metabolic syndrome patients with age matching to the control group (35 - 65 years). The study included the measurement of asprosin hormone. MetS were diagnosed in compliance with the European Group for the Study of Insulin Resistance (EGIR) criteria for patients attending the abdominal consultation unit at the Ibn Sina Teaching Hospital in Mosul, Iraq. Results: The findings revealed a significant increase in the concertation of asprosin hormone in metabolic syndrome patients compared to the control group. Also, it has been found that was a significant increase in the concertation of glucose, insulin, homeostasis model for insulin resistance (HOMA-IR), Glucose to insulin Ratio(G/I), Triglyceride Glucose index (TyG), and McAuley Index, in addition to decreasing in homeostasis model for ß-cell function(HOMA-%ß), sensitivity of insulin(HOMA-%S) and Quantitative insulin sensitivity check index(QUICKI) in the metabolic syndrome patients. there is also a significant positive correlation between asprosin hormone with insulin resistance. Conclusion: Findings indicated that serum levels of asprosin and insulin resistance are increased in patients with metabolic syndrome. Also, they have a relationship with clinical parameters. So asprosin hormone can be used new biomarker of metabolic turbulence. [ABSTRACT FROM AUTHOR]

Objectives: This study aims to examine the association between the (rs1544410) polymorphism of the VDR gene with the pathogenesis of T2DM in Iraqi obese women. Methods: A case-control study was performed on 50 patients with T2DM and 50 apparently healthy subjects who were admitted to Al-Hussein Teaching Hospital and Al-Hassan Center of Diabetes and Endocrinology unit/Kerbala health directorate--Iraq during (April 2022-March 2023). The T2DM groups were divided into two groups, 25 obese and 25 non-obese; the control group was divided into 25 obese and 25 non-obese as apparently healthy groups. The ELISA Kit was used to measure serum 25(OH)D3, heat shock protein-70, VDBP, insulin and C-peptide. Also HbA1c% and insulin resistance (HOMA-IR) were evaluated. The vitamin D3 receptor gene (VDR) variant and the SNP (rs1544410) polymorphism was determined using allele specific polymerase chain reaction, 1.5% agarose gel electrophoresis and then visualized by gel photo-documentation system. Results: The result of vitamin D3 variants genotype (rs 1544410) was a clear band with a molecular size of 200 bps. The size of the amplicon was determined by compare with DNA ladder 100-1500 bp. The result of the comparison between observed and anticipated values for SNIP with (rs 1544410) in the tested population was statistically significant, P = < 0.001 and the difference between demographic characteristics and (rs 1544410) SNP, age and BMI shows non-significant difference among all groups. The difference between biomarkers and (rs1544410) SNP was performed using one-way ANOVA test to compare the mean levels of HSP-70, VDBP, C-peptide, RBC and HbA1c% which shown a non-significant difference among the variants of VDBP Genotype (rs1544410) in obese women (patients and control) studied groups, P value > 0.05. Conclusion: The logistic analysis of the (rs1544410) SNP of the patients concluded that HSP-70, VDBP, and C-peptide level was no significantly related to the also C-peptide, was shown to be a related risk factor to both CT and CT alleles (1.003, P > 0.05) in comparison with CC alleles. Furthermore, HbA1c% level was demonstrated to be related as a risk factor for the CG allele in comparison with CC and GG alleles (1.009, P < 0.05). [ABSTRACT FROM AUTHOR]