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Participatory varietal selection was conducted in Southwestern part of Ethiopia to evaluate the performance of banana cultivars and to identify farmers’ preference and selection criteria in the study area. Seven banana cultivars collected from Melkassa agricultural research center were evaluated in single plot in 2020-2023 main cropping season. Farmers’ evaluation was made at two different stages of the crop, namely at vegetative and at maturity using both direct-matrix and pair-wise ranking methods of selection scheme. Farmers’ set; plant height, disease resistance/tolerance, fruit size and yield as selection criteria to evaluate and identify their preferred cultivars. The results of analysis of variance indicated the existence of highly significant differences among cultivars for all traits measured at 5% probability level. From the previous experiments, the highest mean yield was obtained from the cultivar William I (45.32 t/ha), Dinke-1 (45.1 t/ha), Lady Finger (40.16 t/ha) and Poyo (40 t/ha). Likewise, these three cultivars (William, Dinke-1, and Lady Finger) were identified as farmers preferred cultivars from both pair-wise and direct matrix ranking evaluations. Thus, these cultivars were chosen for their performance in the field and from farmers' evaluation perspective. Moreover, this study indicated participatory varietal selection is a viable method to gain greater insight into farmers’ perceptions, preferences, merits and shortcomings of banana cultivars. Therefore, based on the results of this study, William I, Dinke-1 and Lady Finger cultivars are recommended for multiplication and distribution to farmers in the area and similar agro-ecologies.

Abstract Coagulation factor XII (FXII) conveys various functions as an active protease that promotes thrombosis and inflammation, and as a zymogen via surface receptors like urokinase-type plasminogen activator receptor (uPAR). While plasma levels of FXII are increased in diabetes mellitus and diabetic kidney disease (DKD), a pathogenic role of FXII in DKD remains unknown. Here we show that FXII is locally expressed in kidney tubular cells and that urinary FXII correlates with kidney dysfunction in DKD patients. F12-deficient mice (F12 -/- ) are protected from hyperglycemia-induced kidney injury. Mechanistically, FXII interacts with uPAR on tubular cells promoting integrin β1-dependent signaling. This signaling axis induces oxidative stress, persistent DNA damage and senescence. Blocking uPAR or integrin β1 ameliorates FXII-induced tubular cell injury. Our findings demonstrate that FXII-uPAR-integrin β1 signaling on tubular cells drives senescence. These findings imply previously undescribed diagnostic and therapeutic approaches to detect or treat DKD and possibly other senescence-associated diseases.

Abstract Background The burden of malaria persists in sub-Saharan Africa and the emergence of artemisinin resistance has introduced complexity to control efforts. Monitoring the efficacy of artemisinin-based treatment for malaria is crucial to address this challenge. This study assessed treatment efficacy of artemether-lumefantrine (AL) and genetic diversity of Plasmodium falciparum isolates in a Nigerian population. Methods Participants presenting with clinical symptoms of uncomplicated malaria at a health centre in Lagos, Nigeria, were screened for P. falciparum. Enrolled participants were treated with AL and monitored through scheduled check-up visits, clinical and laboratory examinations for 28 days. Parasite clearance and genetic diversity were assessed through polymerase chain reaction (PCR) analysis of merozoite surface proteins (msp1 and msp2). The prevalence of drug resistance mutations was assessed by P. falciparum multidrug resistance gene 1 (mdr1) genotyping followed by P. falciparum ubiquitin-specific protease 1 (ubp1) gene sequencing. Results The PCR-uncorrected treatment outcome revealed 94.4% adequate clinical and parasitological response (ACPR) and 5.6% late parasitological failure (LPF) rates. After PCR correction, no suspected LPF case was detected and ACPR 67/67 (100%) was achieved in all the individuals. Moreover, a high prevalence of wild-type alleles for mdr1 N86Y (93.7%), and mdr1 D1246Y (87.5%) was observed. Genetic diversity analysis revealed predominant K1 allelic family for msp1 (90.2%) and FC27 for msp2 (64.4%). Estimated multiplicity of infection (MOI) was 1.7, with the highest MOI observed in the 5–15 years age group. ubp1 sequence analysis identified one nonsynonymous E1528D polymorphism at a low frequency (1.6%). Conclusion The study demonstrated sustained efficacy of AL for treating uncomplicated P. falciparum malaria. Genetic diversity analysis revealed various allelic types, suggesting occurrences of polyclonal infections. Nonetheless, the detection of a significant ubp1 polymorphism could have future implications for the epidemiology of anti-malarial drug resistance in the population.

OBJECTIVES/GOALS: Despite a disproportionate impact of COVID-19 on minority and under-resourced communities, nearly all COVID-19 resources have only been online in English. A statewide coalition of community and academic partners used community-engaged strategies to provide tailored outreach to diverse populations. METHODS/STUDY POPULATION: The STOP COVID-19 CA statewide team had a workgroup focused on communications. Members of this group represented different sectors, racial/ethnic groups, disciplines, and regions across the state. They had regular meetings to discuss and strategize how to overcome the impact of historic and structural racism on access to COVID-19 resources, including testing, vaccines, and protective equipment. The team also shared regular updates about changes in community concerns and needs as well as new, tailored resources. RESULTS/ANTICIPATED RESULTS: Together, the team has been able to reach diverse populations across the state, including providing information about COVID-19 in multiple languages and formats, from radio to virtual town halls to local health fairs. The multiple sites also increased access to vaccines and testing through trusted community leaders and locations, including church-based locations to bringing vaccines and testing directly to workplaces. These community pop-up vaccination sites have helped to vaccinate large numbers of diverse populations, some of whom were initially unsure about getting the vaccine, which has helped to reduce the gaps in community vaccination rates by race/ethnicity. DISCUSSION/SIGNIFICANCE: This network of community-engaged strategies utilized for rapid COVID-19 response could also be used to for responses to future public health emergencies, addressing chronic diseases (e.g., diabetes, hypertension), or even other complex issues that affect society and health (e.g., climate change).

Three hot pepper varieties were evaluated in single plot in 2022/23 main cropping season. Farmers’ evaluation was made at two different stages of the crop, namely at vegetative and at maturity using both direct-matrix and pair-wise ranking methods of selection scheme. Farmers’ set; green pod yield, disease tolerance, pod color and size as selection criteria to evaluate and identify their preferred varieties. The results of analysis of variance indicated the existence of highly significant differences among varieties for all traits measured at 5% probability level. The highest mean green pod yield was obtained from the variety Melka Awaze (11.39 t/ha) and Melka Zala (8.39 t/ha). Likewise, these two varieties were identified as farmers preferred varieties from both pair-wise and direct matrix ranking evaluations. Thus, the varieties Melka Awaze and Melka Zala were chosen for their performance in the field and from farmers' evaluation perspective. Moreover, this study indicated participatory varietal selection is a viable method to gain greater insight into farmers’ perceptions, preferences, merits and shortcomings of hot pepper varieties. Therefore, based on the results of this study, Melka Awaze and Melka Zala varieties are recommended for multiplication and distribution to farmers in the area.

Tomato is a crop of immense economic importance worldwide and salinity is one of the major abiotic factors limiting its production and productivity in Ethiopia. The study was conducted to assess growth, physiological activities and yield responses of two tomato varieties to six different salinity levels. Evaluation of the varieties for salt tolerance was carried out in greenhouse in 2018/19. Each treatment was replicated three times and arranged in Randomized Complete Block Design in factorial arrangement. Most of the traits showed significant decrease (P

The fruit with the most commercial significance in the Caricaceae family is the papaya (Carica papaya L.). The fruits are very valuable in terms of nutrition, economy, and medicine. One of the things restricting its production and productivity in Ethiopia is the lack of improved cultivars. In order to determine the optimum high yielding, pest and disease tolerant, variety/ies for the target area, this study was carried out to evaluate the performance of improved papaya varieties. Three newly released papaya varieties were brought to Teppi agricultural research center from Melkassa agricultural research center for evaluation over the cropping seasons of 2019/20 to 2020/21. The experiment was laid out in randomized complete block design and replicated three times. The mean yield was ranged from 34.24 to 90.08 t/ha for Braz-HS1 and Meki-HL1 varieties respectively. The findings of the study revealed that, based on the recorded parameters Meki-HL1 variety performed better followed by Koka-HM1 variety and these two varieties will be recommended for the surrounding farmers for wider production. Further study should be carried out with improved varieties to improve papaya production and nutritional security as well, especially in southwestern Ethiopia.

Abstract BackgroundNoncommunicable diseases continue to pose a substantial health challenge globally, with hyperglycemia serving as a prominent indicator of diabetes. ObjectiveThis study employed machine learning algorithms to predict hyperglycemia in a cohort of individuals who were asymptomatic and unraveled crucial predictors contributing to early risk identification. MethodsThis dataset included an extensive array of clinical and demographic data obtained from 195 adults who were asymptomatic and residing in a suburban community in Nigeria. The study conducted a thorough comparison of multiple machine learning algorithms to ascertain the most effective model for predicting hyperglycemia. Moreover, we explored feature importance to pinpoint correlates of high blood glucose levels within the cohort. ResultsElevated blood pressure and prehypertension were recorded in 8 (4.1%) and 18 (9.2%) of the 195 participants, respectively. A total of 41 (21%) participants presented with hypertension, of which 34 (83%) were female. However, sex adjustment showed that 34 of 118 (28.8%) female participants and 7 of 77 (9%) male participants had hypertension. Age-based analysis revealed an inverse relationship between normotension and age (rPrPF1 ConclusionsThe random forest classifier identified significant clinical correlates associated with hyperglycemia, offering valuable insights for the early detection of diabetes and informing the design and deployment of therapeutic interventions. However, to achieve a more comprehensive understanding of each feature’s contribution to blood glucose levels, modeling additional relevant clinical features in larger datasets could be beneficial.

This article describes a first-person qualitative research study to understand how common pedagogical approaches and cultural learning environments in STEM impact individuals. Prior to the study, the author observed that many students who were successful in advanced undergraduate neuroscience courses reported having struggled academically, socially, or emotionally in introductory STEM courses. The objective was to generate new ideas for approaches to address high rates of student attrition from introductory STEM courses related to this full range of issues through curriculum development. The author, a neurobiologist and tenured faculty member at the institution, audited four introductory STEM courses: Introduction to Cellular and Molecular Biology, Atoms & Molecules, Calculus 1, and Introductory Physics 2: Electromagnetism, Optics, and Modern Physics, offered by tenured colleagues in four different departments. A total of approximately 600 hours was spent by the author attending lectures, participating in classroom activities, completing homework, and studying for assessments. Homework, quizzes, and exams were marked by the course faculty using the same criteria as were applied for student work. In addition to measures of academic performance collected through the normal assessments, the author made note of her own emotional responses throughout the course of the study, which is why the process was dubbed ‘embodied’ curriculum mapping. The emotional responses revealed high levels of emotional stress associated with assessment, sensitivity to disciplinary boundary reinforcement, and a complex role of social and academic identity in all aspects of the experience. Given the first-person nature of the study, the potential future generalizability of the findings must be considered in light of the various revealed aspects of identity and experience of the author and subjected to further study using a broader range of empirical methodologies. The focus of this article’s conclusions and recommendations is therefore the impact of the process on the author and the potential for a similar process to serve as a foundation for critical self-reflection and learning for other STEM educators. The author recommends the process as a generative tool for pedagogical innovation and building faculty capacity for culture change in STEM.

Background: Aging implies changes in terms of lung function, immune system, and respiratory and extra-respiratory comorbidities. Few studies have specifically addressed the relevance of age on severe asthma burden and control. We aimed to evaluate whether age acts as an independent determinant of asthma severity, in terms of clinical, functional, and inflammatory profile, and to explore potential cofactors that contribute to a more difficult disease control in different age groups. Methods: Patients from Severe Asthma Network Italy (SANI) registry were retrospectively divided in subgroups according to their age. Cutoffs for age were established according to quartiles in order to obtain a comparable number of patients for each group, and then rounded for the sake of simplicity. Results: Overall, 1805 severe asthma patients were analyzed. Lung function represented the most important age-related variable. On the opposite the level of asthma control was not differently distributed among age ranges. In young people the presence of atopy-related comorbidities (allergic rhinitis, atopic dermatitis) predominated, whilst systemic-metabolic and degenerative comorbidities such as diabetes, cardiovascular diseases, anxious-depressive syndrome, and osteoporosis prevailed in elderly. Bronchiectasis and sleep disturbances were significantly associated with age. Conclusions: Despite that it cannot be considered a treatable trait, our study suggests that age should be evaluated within a personalized approach to severe asthma patients, in order to provide a better clinical profiling and a more tailored treatment strategy.

Lack of trust in biomedical research, government, and health care systems, especially among racial/ethnic minorities and under-resourced communities, is a longstanding issue rooted in social injustice. The COVID-19 pandemic has further highlighted existing health and socioeconomic inequities and increased the urgency for solutions to provide access to timely, culturally, and linguistically appropriate evidence-based information about COVID-19; and ultimately to promote vaccine uptake. California's statewide alliance STOP COVID-19 CA (comprising eleven sites), leverages long standing community partnerships to better understand concerns, misinformation, and address racial/ethnic inequities in vaccine hesitancy and uptake. Using data from the California CEAL Communication Working Group, we demonstrate the wide range of strategies, communication methods, languages, and trusted messengers that have been effective in reaching diverse communities across the state. We also showcase challenges and lessons learned, such as the importance of including trusted community partners to share information or provide vaccines. These approaches, rooted in community engagement, are crucial for addressing inequities and responding to future public health emergencies.

Abstract Big climate change data have become a pressing issue that organizations face with methods to analyze data generated from various data types. Moreover, storage, processing, and analysis of data generated from climate change activities are becoming very massive, and are challenging for the current algorithms to handle. Therefore, big data analytics methods are designed for significantly large amounts of data required to enhance seasonal change monitoring and understand and ascertain the health risks of climate change. In addition, analysis of climate change data would improve the allocation, and utilisation of natural resources. This paper provides an extensive discussion of big data analytic methods for climate data analysis and investigates how climate change and sustainability issues can be analyzed through these approaches. We further present the big data analytic methods, strengths, and weaknesses, and the essence of analyzing big climate change using these methods. The common datasets, implementation frameworks for climate change modeling, and future research directions were also presented to enhance the clarity of these compelling climate change analysis challenges. This big data analytics method is well-timed to solve the inherent issues of data analysis and easy realization of sustainable development goals.

This study examined extension agents’ viewpoints on how funders’ mandates and access to funds influence sustainable development in Ogun State. Simple random sampling was used to select 80 extension agents, and the data collected were analysed with frequency count, percentages and mean statistics. Findings revealed that eleven projects were funded, mainly by IITA (4) and the World Bank (3). The major challenges were too many instructions and requirements for accessing funds and a short project timeframe. Most (82.5%) of the respondents perceived that funders’ mandates and fund access impede sustainable development. It is concluded that the stridency of accessing funds and fund mandates could make agricultural projects fail. It is recommended that funders and funded organizations address these issues for the smooth implementation of sustainable agriculture and development.

Campuses of Nigerian universities, especially the older ones, are home to aged trees that were originally planted for environmental beautification and aesthetics. However, due to the current global climate change and increased vulnerability to abiotic and biotic stressors, the old trees could pose threats to pedestrians, structures, and roads within the university campus environments. Therefore, the objective of this paper is to develop a geodatabase and evaluate the health risk assessment of avenue trees on selected roads in a tertiary institution in Ibadan, Oyo State, Nigeria using appropriate standard methods. Results obtained reported a total of 121 individual avenue trees belonging to 14 species along the study roads. The geospatial distribution analysis revealed that Emotan road had a lower density of avenue trees compared to Benue and Oduduwa roads. Furthermore, the health risk assessment indicated that 17.35% of the individual avenue trees had defects, posing potential hazards and risks of tree failure, and potential damage to pedestrians, vehicles, and neighboring utilities. The developed geodatabase is user-friendly and allows for easy data storage and quick information retrieval on the avenue trees, enhancing their maintenance and risk management. Furthermore, this study shows that systematic replacement, replanting, and management of avenue tree species could be a proactive initiative for the expansion of the geodatabase and to reduce negative health impacts.

BackgroundNutrition knowledge stands as a cornerstone in facilitating informed dietary choices, thereby profoundly impacting overall health and lifestyle outcomes. Malnutrition often correlates with deficient nutritional knowledge, highlighting the critical need for comprehensive understanding in this domain. While Ethiopia has seen considerable research on nutritional status and associated factors, there remains a paucity of studies specifically addressing nutrition knowledge among secondary school students, particularly within the Haramaya District. Therefore, this study aimed to meticulously assess nutrition knowledge and its determinants among secondary school students in Eastern Ethiopia.MethodsEmploying an institutional-based cross-sectional design, we carefully selected 417 students from secondary schools in Haramaya District, Eastern Ethiopia, through simple random sampling. Data Research Topic entailed structured interviews, with subsequent entry into Epi Data version 3.1 for meticulous analysis utilizing SPSS version 21 software. Descriptive statistics summarized participant characteristics, while both bivariable and multivariable logistic regression analyses were conducted to elucidate factors associated with nutritional knowledge, setting statistical significance at p-value

A college education can help pave the way for a meaningful and rewarding career. However, college students with disabilities, especially students from ethnic and minority backgrounds, face many challenges as they transition from high school to college. Research has demonstrated the importance of social support from family members, friends, and significant others on college life adjustment of minority students with disabilities. The purpose of this study was to validate the "Multidimensional Scale of Perceived Social Support" (MSPSS) for use with African American college students with disabilities. The confirmatory factor analysis was conducted with 220 African American college students with disabilities. The results indicated that the three-factor measurement structure of the MSPSS fits the data relatively well: : X[superscript 2](51, N = 202) = 151.59, p < 0.001; X[superscript 2]/df = 2.97 (< 3), CFI = 0.96 (> 0.95), TLI = 0.95 (>= 0.95), RMSEA = 0.099 (> 0.080), and SRMR = 0.04 (< 0.08). The three factors include perceived social support from (a) family, (b) friends, and (c) significant others. The results of the correlational analysis found significant relationships among these subscales as well as related constructs including goal persistence and college adjustment problems in expected directions providing initial support for the construct validity of the MSPSS. Assessing the levels of perceived social support received by African American students with disabilities can provide valuable information for disability service personnel to tailor their services to help these students develop social skills, social support, and social capital that are critical to college life adjustment and goal persistence.

Background: Vaccines, including COVID-19 vaccines, are known to be cost-effective interventions for disease prevention and control. However, adverse events following immunisation (AEFI) may challenge the acceptance of these vaccines. This study assessed the prevalence and severity of COVID-19-related AEFI amongst healthcare workers at tertiary health facilities in Nigeria. Materials and Methods: This descriptive, cross-sectional study was conducted among healthcare workers who had received the COVID-19 vaccine. A multi-stage sampling technique was used to select participants from six Tertiary Health Facilities in Nigeria. Ethical approval (NHREC/01/01/2007-19/07/2021) was obtained from NHREC. Data were analysed using IBM® SPSS version 25 and categorical variables were presented in tables/charts using frequencies and proportions. Results: A total of 2130 respondents participated in the study, with a mean age of 37.4 ± 9.1 years. Most of the respondents, 1674 (78.6%), had two doses of the vaccine, and the overall prevalence of AEFI was 813 (38.2%). Common among the AEFI reported following the administration of the first dose of the vaccine were fever 649 (30.5%) and pain at the injection site 644 (30.2%), while it was pain at the injection site 216 (10.1%) and fever 173 (8.1%) for second dose. The higher proportions of AEFI were mostly mild to moderate. Conclusion: The study observed a relatively low prevalence of AEFI, with the commonly reported ones being fever and injection site pain. It is crucial that countries continuously collect the data on AEFI and establish causality as a way to improve quality and guarantee vaccine safety.

Tomato (Lycopersicon esculentum L.) belongs to the Solanaceae family. It is a crop of immense economic importance worldwide and salinity is one of the major abiotic factors limiting it's production and productivity globally. The amount of irrigation water and their evapotranspiration is the main reason that causes salinization. Salinity is an abiotic stress that affects agriculture by severely impacting crop growth and, consequently, final yield. Considering that sea levels rise at an alarming rate over year, it is clear that salt stress constitutes a top-ranking threat to agriculture. Among the economically important crops that are sensitive to high salinity is tomato one that is more affected by salt stress. Si plays the beneficial role of the quasi-essential metalloid silicon (Si), which increases the vigor and protects plants against a biotic stresses. The use of silicon fertilization can be used as sustainable practices in agricultural production to increase yield and quality of plants. Silicon fertilization also plays role in plant protection against various range of exogenous stresses especially, under changing environment. The use of appropriate irrigation method, amount and water quality to minimize the risk of salt accumulation around root zone of plants. Different plant growth regulators and amino acids could also play a great role in increasing yield and growth of tomato under salt stress.

Abstract Background Underdiagnosis of migraine causes a significant health burden, including lower quality of life, excessive medication use, and a delay in effective treatment. The purpose of this study was to evaluate migraine diagnosis accuracy and to review the treatment approaches used by neurologists in the Baltic states. Methods The research was conducted as an anonymous e-survey with four cases in March and April 2021. Results 119 practicing adult neurologists have participated. The migraine diagnostic accuracy was 63.2%. The most commonly used diagnostic criteria were moderate/severe pain, unilateral pain, and disruption of daily activities. Diagnostic accuracy did not differ significantly between neurologists who always use ICHD-3 criteria and those who don’t (68.4% vs. 58.5%, p = 0.167). It was higher in neurologists who were working in headache centers (91.7% vs. 60.9%, p = 0.012), and was related to a higher percentage of migraine diagnoses in all consulted headache patients (R 2 = 0.202, adjusted R 2 = 0.195, p

ABSTRACTBanana belongs to the (Musaceae) family. It is a crop of immense economic importance worldwide. The aim of this work was to evaluate the performance of Banana cultivars for yield and yield components. Nine banana cultivars and one standard check cultivar were compared in Tepi agricultural research center. The experiment was laid out in a randomized complete block design with three replications. The analysis of variance showed that, for all phonological and vegetative traits except leaf width, significant variation has been recorded. The combined analysis of the over seasons revealed significant difference due to cultivars and years for most of the characters tested. The over years combined analyses of variance revealed that there were significant differences (P

ABSTRACTAvocado is a member of the Lauraceae family. It is a crop with enormous economic significance all over the world. The aim of this work was to evaluate the performance of avocado cultivars for yield and yield components. Six avocado cultivars and one local check cultivar were compared in Teppi Agricultural Research Center. The experiment was laid out in randomized complete block design with three replications. The analysis of variance showed that, for most growth traits except girth above union and girth below union, significant variation has been recorded. The combined analysis over seasons revealed that significant difference between cultivars for most of the characters tested. The over years combined analyses of variance revealed that there was significant difference (P

This study assessed tree growth variables, above (AGB), below ground biomass (BGB) and total carbon content (TC) sequestered by Parkia biglobosa (Jacq.) Bench. Plantation in Wasangare, Oyo State using non-destructive ground base survey. Tree growth data (Diameter at breast height, Dbh and Tree height, Th) were collected using lacer ace hypsometer and diameter girth tape from 20 temporary sampling plots of size 25 m X 25 m established through systematic transect lines. Diameter size classes (DSC) for the plantation was examined, carbon stock for each DSC was also determine while basal area (m2 ha-1), volume (m3 ha-1), Biomass (Mg ha-1) and Carbon (Mg ha-1) were also estimated. Results showed mean Dbh of 18.7 + 0.25 cm with 8.14 + 0.10 m, 0.033 + 0.00 m2 ha-1 and 0.320 + 0.01 m3 ha-1 for tree height, basal area and volume respectively. AGB and BGB were 10.877 + 0.39 Mgha-1 and 2.175 + 0.08 Mgha-1 respectively while TC was 6.527 + 0.24 Mgha-1. The percentage carbon stock proportion for each DSC revealed class size 25-29-9 cm (19.02%) as the highest while the least proportion was observed in less than 5 cm class with 0.04% of carbon. The DSC showed majority of the tree Dbh in lower Dbh classes with fewer trees in higher classes forming almost a normal bell shape. The study provides information that can help the management in planning silvicultural activities and selective removal from the stand (harvesting schedule). Tree Dbh, height, basal area, volume and biomass are the determinant characteristics for forest carbon assessment. In conclusion, the plantation actively sequesters carbon showing potentials for indigenous trees in climate change mitigation.

ABSTRACT: This study aimed to determine the impact of the age of breeder hens and egg storage on egg quality, embryonic development, hatching events and chick quality in FUNAAB-alpha chickens. The study involved the use of 500 hatching eggs each collected from 32-wk and 60-wk-old of FUNAAB-alpha broiler breeder hens at the Animal Breeding and Genetic Unit of the Federal University of Agriculture, Abeokuta, Nigeria and subjected to 5 storage periods (0, 3, 7, 11, and 15 d). The quality traits of the eggs were recorded and incubated using the conventional protocol. Data were collected on the internal and external egg characteristics, embryonic development, hatching events, and chick quality. The data collected were laid out in 2 by 5 factorial design. The results showed that eggs from 32-wk-old breeder hens had higher albumen height and Haugh unit (HU) value than those from 60-wk-old breeders. The albumen height and HU decreased progressively with storage length in the 2 age groups. Extended storage duration linearly increased (P < 0.01) egg weight loss and decreased (P < 0.01) yolk height. The eggs from both breeder ages had increasing blastodermal diameters at oviposition up until d 11 of storage but decreased on d 15 of storage in eggs from 32 wk breeders. Eggs of 32-wk-old FUNAAB-alpha breeder hens had larger diameters at oviposition compared with 60-wk-old breeders. The chicks from 60-wk breeder had late internal pipping (469.06 h), early external pipping (474.46 h) and a shorter time lag between both pips (9.00 h) compared to chicks from 32 wk breeder. The highest fertility was recorded in eggs stored for 3 d (80.7% and 79.6%), while the lowest fertility was in eggs stored for 15 d (53.4% and 47.7%) in both 32-wk and 60-wk-old breeders, respectively. Chicks from young breeder hens stored for 3 d had better quality scores (100%) compared to 0, 7, 11, and 15-d storage duration and in 60-wk-old breeders across all storage duration. It was concluded that both egg storage duration and age of breeder affected egg quality, hatching events and hatchling quality of FUNAAB-alpha chickens and the interaction effects of both factors was recorded for some of these traits. However, extended storage beyond 7 d had a larger negative impact on egg quality and hatchability of eggs from an old breeder (60 wk) than on eggs of a young breeder (32 wk).

OBJECTIVES/GOALS: Despite a disproportionate impact of COVID-19 on minority and under-resourced communities, nearly all COVID-19 resources have only been online in English. A statewide coalition of community and academic partners used community-engaged strategies to provide tailored outreach to diverse populations. METHODS/STUDY POPULATION: The STOP COVID-19 CA statewide team had a workgroup focused on communications. Members of this group represented different sectors, racial/ethnic groups, disciplines, and regions across the state. They had regular meetings to discuss and strategize how to overcome the impact of historic and structural racism on access to COVID-19 resources, including testing, vaccines, and protective equipment. The team also shared regular updates about changes in community concerns and needs as well as new, tailored resources. RESULTS/ANTICIPATED RESULTS: Together, the team has been able to reach diverse populations across the state, including providing information about COVID-19 in multiple languages and formats, from radio to virtual town halls to local health fairs. The multiple sites also increased access to vaccines and testing through trusted community leaders and locations, including church-based locations to bringing vaccines and testing directly to workplaces. These community pop-up vaccination sites have helped to vaccinate large numbers of diverse populations, some of whom were initially unsure about getting the vaccine, which has helped to reduce the gaps in community vaccination rates by race/ethnicity. DISCUSSION/SIGNIFICANCE: This network of community-engaged strategies utilized for rapid COVID-19 response could also be used to for responses to future public health emergencies, addressing chronic diseases (e.g., diabetes, hypertension), or even other complex issues that affect society and health (e.g., climate change).

Background: This study aimed to assess the contamination of Polychlorinated Biphenyls (PCBs) in indoor dust among university students in Ilorin, Nigeria. Methods: One hundred (100) indoor dust samples were collected from different locations within university hostels. Two Universities in Ilorin were sampled for the study; the Univerisity of Ilorin and Al-Hikmah University. Gas chromatography-mass spectroscopy (GC-MS) was employed to analyze and identify fourteen PCB congeners. Results: The results revealed that university students were exposed to low levels of PCB contamination in indoor dust. The concentrations of individual PCB congeners ranged from 4.993ng/g to 37.701ng/g. Notably, PCB-52 and PCB-118 were the predominant congeners, with mean concentrations of 37.701ng/g and 30.928ng/g. Conversely, PCB-44 exhibited the lowest occurrence, with a mean concentration of 4.993ng/g. The study found positive correlations among specific PCB congeners, suggesting familiar sources and environmental behaviors. However, no significant correlations were found between residential characteristics and measured PCB levels. Conclusion: The results emphasize the need for further research to minimize PCB contamination in indoor environments and protect human health. It is recommended that PCB levels be monitored in indoor environments and enforce strong regulations to control PCB production, use, and disposal.

Abstract Purpose One of the main limiting factors of whole-brain radiation therapy (WBRT) for primary central nervous system lymphoma (PCNSL) is the impairment of neurocognitive functions (NCFs), which is mainly caused by radiation-induced injury to the hippocampus. With a view to preventing NCF impairment and personalizing treatment, we explored the feasibility of sparing the hippocampus during WBRT by correlating the sites of PCNSL lesions with the hippocampus. Methods and materials Pre-treatment MR images from patients who underwent WBRT between 2010 and January 2020—and post-radiotherapy images in cases of relapse—were imported into the Varian Eclipse treatment-planning system and registered with the simulation CT. We constructed three 3-dimensional envelopes around the hippocampus at distances of 5, 10 and 15 mm and also contoured primary lesions and recurrences. Results We analyzed 43 patients with 66 primary lesions: 9/66 (13.6%) involved the hippocampus and 11/66 (16.7%) were located within 5 mm of it. Thirty-six lesions (54.5%) were situated more than 15 mm from the hippocampus, while 10/66 (15.2%) were between 5 and 15 mm from it. The most common location was in deep brain structures (31%). Thirty-five of the 66 lesions relapsed: in field in 14/35 (40%) and outfield in 21/35 (60%) in different sites. Globally, 16/35 recurrences (45.7%) were located in the hippocampus or within 5 mm of it. Conclusion These data show that routinely sparing the hippocampus is not feasible. This approach could be considered in selected patients, when the lesion is more than 15 mm from the hippocampus.

The primary reason for postharvest deterioration, which is primarily brought on by fungal invasion, is the loss of turmeric products in Southwestern Ethiopia. The study was conducted to determine the main fungi responsible for the postharvest deterioration of turmeric in major turmeric-growing areas of southwestern Ethiopia. In total, 60 samples of dried and stored turmeric were gathered in 2020 and 2021 from different production zones of southwestern Ethiopia. The fungus that causes postharvest turmeric to deteriorate was isolated and identified at the genus level. At the study locations, rhizomes were not cleaned, washed, sorted, or separated from healthy rhizomes. The spoiled turmeric samples contain fungi from four genera: Aspergillus, Penicillium, Fusarium, and Rhizopus. Aspergillus, Penicillium, and Fusarium were isolated from drying and storage samples in all zones. However, Rhizopus was only isolated from samples that had been stored. It was discovered that Aspergillus, Penicillium, and Fusarium were primarily responsible for the observed deterioration. The identified genera's percentage incidence ranged from 15.1% (Fusarium) to 45.2% (Aspergillus). Proper harvesting, postharvest handling procedures, and adopting suitable turmeric harvesting and postharvest handling technology can help decrease turmeric postharvest degradation.

During the COVID-19 pandemic, HIV programs scaled up differentiated service delivery (DSD) models for people living with HIV (PLHIV). We evaluated the effects of COVID-19 on HIV service delivery and viral suppression in facilities in Northern Nigeria, and determined factors associated with viral suppression among adolescents and adults. We analysed a cross-sectional survey data from facility heads, and retrospective, routinely collected patient data from 63 facilities for PLHIV ≥10 years old in care between April 2019-March 2021, defining study periods as "pre-COVID-19" (before April 2020) and "during COVID-19" (after April 2020). For the pre-COVID and the COVID-19 periods we compared uptake of antiretroviral therapy (ART) refills of ≥3 months (MMD3), and ≥6 months (MM6), missed appointments, viral load (VL) testing, VL testing turnaround time (TAT) and viral suppression among those on ART for ≥6 months using two proportions Z-test and t-tests. We fit a multivariable logistic regression model to determine factors associated with maintaining or achieving viral suppression. Of 84,776 patients, 58% were 5 years, 93% from facilities with community-based ART refill, a higher proportion were on MMD3 (95% versus 74%, p

Black pod disease of cocoa (Theobroma cacao L.) caused by Phytophthora palmivora is the major constraint in cocoa production in southwestern Ethiopia. Therefore, integrated field management trials were conducted to evaluate the efficacy of different fungicides against a disease epidemic of cocoa black pod in southwestern Ethiopia. The field experiment was laid out in a randomized complete block design with seven treatments and three replications. The experiment was performed during the 2021 and 2022 main cropping seasons using five fungicides: Copper oxychloride (Koka blue 50 WG) combined with cultural practices, Agro-laxyl 63.5 WP (Metalaxyl + Mancozeb) combined with cultural practices, Progress 250 EC (Propiconazole 25%) combined with cultural practices, Ethiozeb 80% WP (Mancozeb) combined with cultural practices, More 720 WP (Mancozeb +Cymoxanil) combined with cultural practices, cultural practices alone and control without any cultural practices and fungicides spray for control of black pod infection. Cultural practices such as nutrient management, pruning, plant and field hygiene etc. were implemented in all treatments except control. The results of the experiment indicated that copper oxychloride (Koka blue 50 WG) combined with cultural practices significantly affected the cocoa black pod epidemics. When paired with cultural practices, copper oxychloride (Koka blue 50 WG) had a considerably greater impact on lowering disease severity (4.76) than the other treatments. Additionally, compared to control plots, the incidence of disease was reduced by more than 50% when cultural practices alone were used. This shows that in farms where black pod disease severity is low, the disease may be economically managed by implementing cultural measures alone. So, the the study's findings, black pod disease can be controlled in areas with a high disease incidence by combining the application of copper oxychloride (Koka blue 50 WG) with other cultural practices.

Abstract Background Buruli ulcer (BU) is a debilitating neglected tropical disease which causes disability and mostly affects inhabitants in impoverished settings where access to medical care is challenging. This study aims to determine the effect of training community members as volunteers for or in the detection and referral of people who have Buruli ulcer to the hospital. Methods The following study is a before and after study in the BU-endemic Local Government Areas (LGA) of Ebonyi State. A cluster random sampling technique was used to select 90 volunteers from three LGAs (30 from each LGA). In each LGA, the volunteers underwent a one-day training and six months field work to identify all those who have any form of ulcer on any part of their bodies. A short questionnaire was used to capture socio-demographic characteristics of the patient, site of the ulcer, duration of the ulcer, initial appearance of the ulcer, referral to hospital, result of laboratory investigation, and treatment received. The data was analysed using the Statistical Package for Social Sciences (SPSS) for Microsoft Windows version 20 software. The Z test statistic was used to compare the number of referred BU patients before and after the intervention by LGA. The Chi square test was used to examine the association between the dependent and independent variables. Results The mean age of volunteers was 39 ± 9.5 while mean age of the patients was 42.3 ± 17.1. Most of the ulcers were on the legs (79.4%) and lasted 1–5 years (65.6%). There was a significant increase in the proportion of BU suspects identified by the community volunteers in all 3 LGAs (Afikpo north (p =