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194 results on '"Alpizar, Yeranddy A"'

3. Local immune response to food antigens drives meal-induced abdominal pain

Author

Aguilera-Lizarraga, Javier, Florens, Morgane V., Viola, Maria Francesca, Jain, Piyush, Decraecker, Lisse, Appeltans, Iris, Cuende-Estevez, Maria, Fabre, Naomi, Van Beek, Kim, Perna, Eluisa, Balemans, Dafne, Stakenborg, Nathalie, Theofanous, Stavroula, Bosmans, Goele, Mondelaers, Stéphanie U., Matteoli, Gianluca, Ibiza Martínez, Sales, Lopez-Lopez, Cintya, Jaramillo-Polanco, Josue, Talavera, Karel, Alpizar, Yeranddy A., Feyerabend, Thorsten B., Rodewald, Hans-Reimer, Farre, Ricard, Redegeld, Frank A., Si, Jiyeon, Raes, Jeroen, Breynaert, Christine, Schrijvers, Rik, Bosteels, Cédric, Lambrecht, Bart N., Boyd, Scott D., Hoh, Ramona A., Cabooter, Deirdre, Nelis, Maxim, Augustijns, Patrick, Hendrix, Sven, Strid, Jessica, Bisschops, Raf, Reed, David E., Vanner, Stephen J., Denadai-Souza, Alexandre, Wouters, Mira M., and Boeckxstaens, Guy E.

Published
2021
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4. YF17D‐based vaccines – standing on the shoulders of a giant.

Author

Sanchez‐Felipe, Lorena, Alpizar, Yeranddy A., Ma, Ji, Coelmont, Lotte, and Dallmeier, Kai

Subjects
PANDEMIC preparedness, JAPANESE B encephalitis, CHRONIC active hepatitis, YELLOW fever, BIOENGINEERING
Abstract

Summary: Live‐attenuated yellow fever vaccine (YF17D) was developed in the 1930s as the first ever empirically derived human vaccine. Ninety years later, it is still a benchmark for vaccines made today. YF17D triggers a particularly broad and polyfunctional response engaging multiple arms of innate, humoral and cellular immunity. This unique immunogenicity translates into an extraordinary vaccine efficacy and outstanding longevity of protection, possibly by single‐dose immunization. More recently, progress in molecular virology and synthetic biology allowed engineering of YF17D as a powerful vector and promising platform for the development of novel recombinant live vaccines, including two licensed vaccines against Japanese encephalitis and dengue, even in paediatric use. Likewise, numerous chimeric and transgenic preclinical candidates have been described. These include prophylactic vaccines against emerging viral infections (e.g. Lassa, Zika and SARS‐CoV‐2) and parasitic diseases (e.g. malaria), as well as therapeutic applications targeting persistent infections (e.g. HIV and chronic hepatitis), and cancer. Efforts to overcome historical safety concerns and manufacturing challenges are ongoing and pave the way for wider use of YF17D‐based vaccines. In this review, we summarize recent insights regarding YF17D as vaccine platform, and how YF17D‐based vaccines may complement as well as differentiate from other emerging modalities in response to unmet medical needs and for pandemic preparedness. [ABSTRACT FROM AUTHOR]

Published
2024
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5. Editorial: Neuro-immune players of peripheral pain signalling.

Author

Aguilera-Lizarraga, Javier, Reed, David E., Starkl, Philipp, and Alpizar, Yeranddy A.

Subjects
ASPARTATE aminotransferase, BACTERIAL cell walls, EXERCISE physiology, BASIC proteins, DORSAL root ganglia, OROFACIAL pain, TOOTHACHE
Abstract

This article, titled "Editorial: Neuro-immune players of peripheral pain signalling," explores the interactions between the immune and nervous systems in the context of pain. It discusses the role of nociceptors, specialized sensory neurons that respond to harmful stimuli, and how their activation by inflammatory molecules can lead to chronic pain. The article includes two review articles and three original research articles that cover topics such as leukocyte infiltration in neuropathic pain, the effects of exercise on neuroimmune responses, and the mechanisms of pain sensitization. Overall, this research topic provides valuable insights into the complex relationship between the immune and nervous systems in pain signaling. [Extracted from the article]

Published
2024
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12. Acute inhibition of transient receptor potential vanilloid‐type 4 cation channel halts cytoskeletal dynamism in microglia

Author

Beeken, Jolien, primary, Mertens, Melanie, additional, Stas, Nathan, additional, Kessels, Sofie, additional, Aerts, Liese, additional, Janssen, Bieke, additional, Mussen, Femke, additional, Pinto, Silvia, additional, Vennekens, Rudi, additional, Rigo, Jean‐Michel, additional, Nguyen, Laurent, additional, Brône, Bert, additional, and Alpizar, Yeranddy A., additional

Published
2022
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17. p27 kip1 Modulates the Morphology and Phagocytic Activity of Microglia.

Author

Beeken, Jolien, Kessels, Sofie, Rigo, Jean-Michel, Alpizar, Yeranddy A., Nguyen, Laurent, and Brône, Bert

Subjects
MICROGLIA, CELL migration, CELL cycle, MORPHOLOGY, BRAIN damage, PHAGOCYTOSIS, CEREBRAL cortex
Abstract

p27kip1 is a multifunctional protein that promotes cell cycle exit by blocking the activity of cyclin/cyclin-dependent kinase complexes as well as migration and motility via signaling pathways that converge on the actin and microtubule cytoskeleton. Despite the broad characterization of p27kip1 function in neural cells, little is known about its relevance in microglia. Here, we studied the role of p27kip1 in microglia using a combination of in vitro and in situ approaches. While the loss of p27kip1 did not affect microglial density in the cerebral cortex, it altered their morphological complexity in situ. However, despite the presence of p27kip1 in microglial processes, as shown by immunofluorescence in cultured cells, loss of p27kip1 did not change microglial process motility and extension after applying laser-induced brain damage in cortical brain slices. Primary microglia lacking p27kip1 showed increased phagocytic uptake of synaptosomes, while a cell cycle dead variant negatively affected phagocytosis. These findings indicate that p27kip1 plays specific roles in microglia. [ABSTRACT FROM AUTHOR]

Published
2022
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19. Local immune response to food antigens drives meal-induced abdominal pain

Author

Afd Pharmacology, Pharmacology, Aguilera-Lizarraga, Javier, Florens, Morgane V, Viola, Maria Francesca, Jain, Piyush, Decraecker, Lisse, Appeltans, Iris, Cuende-Estevez, Maria, Fabre, Naomi, Van Beek, Kim, Perna, Eluisa, Balemans, Dafne, Stakenborg, Nathalie, Theofanous, Stavroula, Bosmans, Goele, Mondelaers, Stéphanie U, Matteoli, Gianluca, Ibiza Martínez, Sales, Lopez-Lopez, Cintya, Jaramillo-Polanco, Josue, Talavera, Karel, Alpizar, Yeranddy A, Feyerabend, Thorsten B, Rodewald, Hans-Reimer, Farre, Ricard, Redegeld, Frank A, Si, Jiyeon, Raes, Jeroen, Breynaert, Christine, Schrijvers, Rik, Bosteels, Cédric, Lambrecht, Bart N, Boyd, Scott D, Hoh, Ramona A, Cabooter, Deirdre, Nelis, Maxim, Augustijns, Patrick, Hendrix, Sven, Strid, Jessica, Bisschops, Raf, Reed, David E, Vanner, Stephen J, Denadai-Souza, Alexandre, Wouters, Mira M, Boeckxstaens, Guy E, Afd Pharmacology, Pharmacology, Aguilera-Lizarraga, Javier, Florens, Morgane V, Viola, Maria Francesca, Jain, Piyush, Decraecker, Lisse, Appeltans, Iris, Cuende-Estevez, Maria, Fabre, Naomi, Van Beek, Kim, Perna, Eluisa, Balemans, Dafne, Stakenborg, Nathalie, Theofanous, Stavroula, Bosmans, Goele, Mondelaers, Stéphanie U, Matteoli, Gianluca, Ibiza Martínez, Sales, Lopez-Lopez, Cintya, Jaramillo-Polanco, Josue, Talavera, Karel, Alpizar, Yeranddy A, Feyerabend, Thorsten B, Rodewald, Hans-Reimer, Farre, Ricard, Redegeld, Frank A, Si, Jiyeon, Raes, Jeroen, Breynaert, Christine, Schrijvers, Rik, Bosteels, Cédric, Lambrecht, Bart N, Boyd, Scott D, Hoh, Ramona A, Cabooter, Deirdre, Nelis, Maxim, Augustijns, Patrick, Hendrix, Sven, Strid, Jessica, Bisschops, Raf, Reed, David E, Vanner, Stephen J, Denadai-Souza, Alexandre, Wouters, Mira M, and Boeckxstaens, Guy E

Published
2021

24. Crucial Role of Transient Receptor Potential Ankyrin 1 and Mast Cells in Induction of Nonallergic Airway Hyperreactivity in Mice

Author

Hox, Valérie, Vanoirbeek, Jeroen A., Alpizar, Yeranddy A., Voedisch, Sabrina, Callebaut, Ina, Bobic, Sonja, Sharify, Ali, De Vooght, Vanessa, Van Gerven, Laura, Devos, Fien, Liston, Adrian, Voets, Thomas, Vennekens, Rudi, Bullens, Dominique M. A., De Vries, Annick, Hoet, Peter, Braun, Armin, Ceuppens, Jan L., Talavera, Karel, Nemery, Benoit, and Hellings, Peter W.

Published
2013
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27. Effect of resolvins on sensitisation of TRPV1 and visceral hypersensitivity in IBS

Author

Perna, Eluisa, primary, Aguilera-Lizarraga, Javier, additional, Florens, Morgane V, additional, Jain, Piyush, additional, Theofanous, Stavroula A, additional, Hanning, Nikita, additional, De Man, Joris G, additional, Berg, Maya, additional, De Winter, Benedicte, additional, Alpizar, Yeranddy A, additional, Talavera, Karel, additional, Vanden Berghe, Pieter, additional, Wouters, Mira, additional, and Boeckxstaens, Guy, additional

Published
2020
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28. TRPV4 Mediates Acute Bladder Responses to Bacterial Lipopolysaccharides

Author

Alpizar, Yeranddy A., primary, Uvin, Pieter, additional, Naert, Robbe, additional, Franken, Jan, additional, Pinto, Silvia, additional, Sanchez, Alicia, additional, Gevaert, Thomas, additional, Everaerts, Wouter, additional, Voets, Thomas, additional, De Ridder, Dirk, additional, and Talavera, Karel, additional

Published
2020
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30. Mouse TRPA1 function and membrane localization are modulated by direct interactions with cholesterol

Author

Startek, Justyna J.B., Boonen, Brett, López-Requena, Alejandro, Talavera Perez, Ariel, Alpizar, Yeranddy Aguiar, Ghosh, Debapriya, Van Ranst, Nele, Nilius, Bernd, Voets, Thomas, Talavera, Karel, Startek, Justyna J.B., Boonen, Brett, López-Requena, Alejandro, Talavera Perez, Ariel, Alpizar, Yeranddy Aguiar, Ghosh, Debapriya, Van Ranst, Nele, Nilius, Bernd, Voets, Thomas, and Talavera, Karel

Abstract

The cation channel TRPA1 transduces a myriad of noxious chemical stimuli into nociceptor electrical excitation and neuropeptide release, leading to pain and neurogenic inflammation. Despite emergent evidence that TRPA1 is regulated by the membrane environment, it remains unknown whether this channel localizes in membrane microdomains or whether it interacts with cholesterol. Using total internal reflection fluorescence microscopy and density gradient centrifugation we found that mouse TRPA1 localizes preferably into cholesterol-rich domains and functional experiments revealed that cholesterol depletion decreases channel sensitivity to chemical agonists. Moreover, we identified two structural motifs in transmembrane segments 2 and 4 involved in mTRPA1-cholesterol interactions that are necessary for normal agonist sensitivity and plasma membrane localization. We discuss the impact of such interactions on TRPA1 gating mechanisms, regulation by the lipid environment, and role of this channel in sensory membrane microdomains, all of which helps to understand the puzzling pharmacology and pathophysiology of this channel., SCOPUS: ar.j, info:eu-repo/semantics/published

Published
2019

31. Effect of resolvins on sensitisation of TRPV1 and visceral hypersensitivity in IBS.

Author

Perna, Eluisa, Aguilera-Lizarraga, Javier, Florens, Morgane V., Jain, Piyush, Theofanous, Stavroula A., Hanning, Nikita, De Man, Joris G., Berg, Maya, De Winter, Benedicte, Alpizar, Yeranddy A., Talavera, Karel, Berghe, Pieter Vanden, Wouters, Mira, and Boeckxstaens, Guy

Subjects
IRRITABLE colon, TRPV cation channels, G protein coupled receptors, CELL receptors, TRP channels
Published
2021
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36. Preoperative administration of the 5-HT4 receptor agonist prucalopride reduces intestinal inflammation and shortens postoperative ileus via cholinergic enteric neurons

Author

Stakenborg, Nathalie, primary, Labeeuw, Evelien, additional, Gomez-Pinilla, Pedro J, additional, De Schepper, Sebastiaan, additional, Aerts, Raymond, additional, Goverse, Gera, additional, Farro, Giovanna, additional, Appeltans, Iris, additional, Meroni, Elisa, additional, Stakenborg, Michelle, additional, Viola, Maria Francesca, additional, Gonzalez-Dominguez, Erika, additional, Bosmans, Goele, additional, Alpizar, Yeranddy A, additional, Wolthuis, Albert, additional, D’Hoore, Andre, additional, Van Beek, Kim, additional, Verheijden, Simon, additional, Verhaegen, Marleen, additional, Derua, Rita, additional, Waelkens, Etienne, additional, Moretti, Milena, additional, Gotti, Cecilia, additional, Augustijns, Patrick, additional, Talavera, Karel, additional, Vanden Berghe, Pieter, additional, Matteoli, Gianluca, additional, and Boeckxstaens, Guy E, additional

Published
2018
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37. TRP channels as sensors of bacterial endotoxins

Author

Research Foundation - Flanders, KU Leuven, Boonen, Brett, Alpizar, Yeranddy A., Meseguer, Víctor M., Talavera, Karel, Research Foundation - Flanders, KU Leuven, Boonen, Brett, Alpizar, Yeranddy A., Meseguer, Víctor M., and Talavera, Karel

Abstract

The cellular and systemic effects induced by bacterial lipopolysaccharides (LPS) have been solely attributed to the activation of the Toll-like receptor 4 (TLR4) signalling cascade. However, recent studies have shown that LPS activates several members of the Transient Receptor Potential (TRP) family of cation channels. Indeed, LPS induces activation of the broadly-tuned chemosensor TRPA1 in sensory neurons in a TLR4-independent manner, and genetic ablation of this channel reduced mouse pain and inflammatory responses triggered by LPS and the gustatory-mediated avoidance to LPS in fruit flies. LPS was also shown to activate TRPV4 channels in airway epithelial cells, an effect leading to an immediate production of bactericidal nitric oxide and to an increase in ciliary beat frequency. In this review, we discuss the role of TRP channels as sensors of bacterial endotoxins, and therefore, as crucial players in the timely detection of invading gram-negative bacteria.

Published
2018

38. Staphylococcus aureus enterotoxin B disrupts nasal epithelial barrier integrity.

Author

Martens, Katleen, Seys, Sven F., Alpizar, Yeranddy A., Schrijvers, Rik, Bullens, Dominique M. A., Breynaert, Christine, Lebeer, Sarah, and Steelant, Brecht

Subjects
STAPHYLOCOCCUS aureus, ENTEROTOXINS, TIGHT junctions, EPITHELIAL cell culture, EPITHELIAL cells
Abstract

Background: Staphylococcus aureus colonization and release of enterotoxin B (SEB) has been associated with severe chronic rhinosinusitis with nasal polyps (CRSwNP). The pathogenic mechanism of SEB on epithelial barriers, however, is largely unexplored. Objective: We investigated the effect of SEB on nasal epithelial barrier function. Methods: SEB was apically administered to air‐liquid interface (ALI) cultures of primary polyp and nasal epithelial cells of CRSwNP patients and healthy controls, respectively. Epithelial cell integrity and tight junction expression were evaluated. The involvement of Toll‐like receptor 2 (TLR2) activation was studied in vitro with TLR2 monoclonal antibodies and in vivo in tlr2−/− knockout mice. Results: SEB applied to ALI cultures of polyp epithelial cells decreased epithelial cell integrity by diminishing occludin and zonula occludens (ZO)‐1 protein expression. Antagonizing TLR2 prevented SEB‐induced barrier disruption. SEB applied in the nose of control mice increased mucosal permeability and decreased mRNA expression of occludin and ZO‐1, whereas mucosal integrity and tight junction expression remained unaltered in tlr2−/− mice. Furthermore, in vitro SEB stimulation resulted in epithelial production of IL‐6 and IL‐8, which was prevented by TLR2 antagonization. Conclusion & Clinical relevance: SEB damages nasal polyp epithelial cell integrity by triggering TLR2 in CRSwNP. Our results suggest that SEB might represent a driving factor of disease exacerbation, rather than a causal factor for epithelial defects in CRSwNP. Interfering with TLR2 triggering might provide a way to avoid the pathophysiological consequences of S. aureus on inflammation in CRSwNP. [ABSTRACT FROM AUTHOR]

Published
2021
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43. TRPV4 activation triggers protective responses to bacterial lipopolysaccharides in airway epithelial cells

Author

KU Leuven, Research Foundation - Flanders, Agency for Innovation by Science and Technology (Belgium), Ministerio de Economía y Competitividad (España), Instituto de Salud Carlos III, European Commission, Alpizar, Yeranddy A., Boonen, Brett, Sánchez, Alicia, Jung, Carole, López-Requena, Alejandro, Naert, Robbe, Steelant, Brecht, Luyts, Katrien, Plata, Cristina, De Vooght, Vanessa, Vanoirbeek, Jeroen A. J., Meseguer, Víctor M., Voets, Thomas, Álvarez, Julio L., Hellings, Peter W., Hoet, Peter H.M., Nemery, Benoit, Valverde, Miguel A., Talavera, Karel, KU Leuven, Research Foundation - Flanders, Agency for Innovation by Science and Technology (Belgium), Ministerio de Economía y Competitividad (España), Instituto de Salud Carlos III, European Commission, Alpizar, Yeranddy A., Boonen, Brett, Sánchez, Alicia, Jung, Carole, López-Requena, Alejandro, Naert, Robbe, Steelant, Brecht, Luyts, Katrien, Plata, Cristina, De Vooght, Vanessa, Vanoirbeek, Jeroen A. J., Meseguer, Víctor M., Voets, Thomas, Álvarez, Julio L., Hellings, Peter W., Hoet, Peter H.M., Nemery, Benoit, Valverde, Miguel A., and Talavera, Karel

Abstract

Lipopolysaccharides (LPS), the major components of the wall of gram-negative bacteria, trigger powerful defensive responses in the airways via mechanisms thought to rely solely on the Toll-like receptor 4 (TLR4) immune pathway. Here we show that airway epithelial cells display an increase in intracellular Ca2+ concentration within seconds of LPS application. This response occurs in a TLR4-independent manner, via activation of the transient receptor potential vanilloid 4 cation channel (TRPV4). We found that TRPV4 mediates immediate LPS-induced increases in ciliary beat frequency and the production of bactericidal nitric oxide. Upon LPS challenge TRPV4-deficient mice display exacerbated ventilatory changes and recruitment of polymorphonuclear leukocytes into the airways. We conclude that LPS-induced activation of TRPV4 triggers signaling mechanisms that operate faster and independently from the canonical TLR4 immune pathway, leading to immediate protective responses such as direct antimicrobial action, increase in airway clearance, and the regulation of the inflammatory innate immune reaction.

Published
2017

44. TRPV4 activation triggers protective responses to bacterial lipopolysaccharides in airway epithelial cells

Author

Alpizar, Yeranddy A., primary, Boonen, Brett, additional, Sanchez, Alicia, additional, Jung, Carole, additional, López-Requena, Alejandro, additional, Naert, Robbe, additional, Steelant, Brecht, additional, Luyts, Katrien, additional, Plata, Cristina, additional, De Vooght, Vanessa, additional, Vanoirbeek, Jeroen A. J., additional, Meseguer, Victor M., additional, Voets, Thomas, additional, Alvarez, Julio L., additional, Hellings, Peter W., additional, Hoet, Peter H. M., additional, Nemery, Benoit, additional, Valverde, Miguel A., additional, and Talavera, Karel, additional

Published
2017
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47. TRPV1 Contributes to Acrolein-Induced Toxicity

Author

Alpizar, Yeranddy A., primary, Boonen, Brett, additional, Gees, Maarten, additional, Uvin, Pieter, additional, Voets, Thomas, additional, De Ridder, Dirk, additional, Everaerts, Wouter, additional, and Talavera, Karel, additional

Published
2017
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48. Preoperative administration of the 5-HT4 receptor agonist prucalopride reduces intestinal inflammation and shortens postoperative ileus via cholinergic enteric neurons.

Author

Stakenborg, Nathalie, Labeeuw, Evelien, Gomez-Pinilla, Pedro J, Schepper, Sebastiaan De, Aerts, Raymond, Goverse, Gera, Farro, Giovanna, Appeltans, Iris, Meroni, Elisa, Stakenborg, Michelle, Viola, Maria Francesca, Gonzalez-Dominguez, Erika, Bosmans, Goele, Alpizar, Yeranddy A, Wolthuis, Albert, D'Hoore, Andre, Van Beek, Kim, Verheijden, Simon, Verhaegen, Marleen, and Derua, Rita

Subjects
CARBACHOL, NEURONS, MEDICAL ethics, BOWEL obstructions, PANCREATICODUODENECTOMY, ENTERIC nervous system, COLECTOMY, SUBMUCOUS plexus
Published
2019
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49. Neuro-immune interactions in chemical-induced airway hyperreactivity.

Author

KUL - University Hospitals Leuven, Devos, Fien C, Boonen, Brett, Alpizar, Yeranddy A, Maes, Tania, Hox, Valérie, Seys, Sven, Pollaris, Lore, Liston, Adrian, Nemery, Benoit, Talavera, Karel, Hoet, Peter H M, Vanoirbeek, Jeroen A J, KUL - University Hospitals Leuven, Devos, Fien C, Boonen, Brett, Alpizar, Yeranddy A, Maes, Tania, Hox, Valérie, Seys, Sven, Pollaris, Lore, Liston, Adrian, Nemery, Benoit, Talavera, Karel, Hoet, Peter H M, and Vanoirbeek, Jeroen A J

Abstract

Asthma may be induced by chemical sensitisers, via mechanisms that are still poorly understood. This type of asthma is characterised by airway hyperreactivity (AHR) and little airway inflammation. Since potent chemical sensitisers, such as toluene-2,4-diisocyanate (TDI), are also sensory irritants, it is suggested that chemical-induced asthma relies on neuro-immune mechanisms.We investigated the involvement of transient receptor potential channels (TRP) A1 and V1, major chemosensors in the airways, and mast cells, known for their ability to communicate with sensory nerves, in chemical-induced AHR.In vitro intracellular calcium imaging and patch-clamp recordings in TRPA1- and TRPV1-expressing Chinese hamster ovarian cells showed that TDI activates murine TRPA1, but not TRPV1. Using an in vivo model, in which an airway challenge with TDI induces AHR in TDI-sensitised C57Bl/6 mice, we demonstrated that AHR does not develop, despite successful sensitisation, in Trpa1 and Trpv1 knockout mice, and wild-type mice pretreated with a TRPA1 blocker or a substance P receptor antagonist. TDI-induced AHR was also abolished in mast cell deficient Kit(Wsh) (/Wsh) mice, and in wild-type mice pretreated with the mast cell stabiliser ketotifen, without changes in immunological parameters.These data demonstrate that TRPA1, TRPV1 and mast cells play an indispensable role in the development of TDI-elicited AHR.

Published
2016

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