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1. 1. Several samples of Bence-Jones protein were isolated from urine of a patient with multiple myeloma. The isolation was carried out by different methods and from different urine specimens of the same patient. A sample of the same protein was also isolated from serum of the patient. 2. 2. The isoelectric point, acid-base binding capacity, viscosity, optical rotation, and amino acid composition of the various samples were determined. Significant variations in properties and chemical composition were found for several samples. 3. 3. The homogenity of the isolated Bence-Jones protein was determined by paper electrophoresis and by flocculation with propanol. The samples tested were found to give one single component by paper electrophoresis, but behaved like a mixture of proteins in the floccutation test.

1. 1. The distribution pattern of free amino acids and related substances (taurine, glutathione and glutamine), has been determined by means of two-dimensional paper chromatography in eight organs of the rat. 2. 2. A major portion of the amino nitrogen was found distributed among few substances, in a manner characteristic for each organ. Several unidentified substances have been detected in some of the tissues examined.