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154 results on '"Alves, Gutemberg Gomes"'

1. Investigating the Biological Efficacy of Albumin-Enriched Platelet-Rich Fibrin (Alb-PRF): A Study on Cytokine Dynamics and Osteoblast Behavior.

Author

Lourenço, Emanuelle Stellet, Rocha, Neilane Rodrigues Santiago, de Lima Barbosa, Renata, Mello-Machado, Rafael Coutinho, de Souza Lima, Victor Hugo, Leite, Paulo Emilio Correa, Pereira, Mariana Rodrigues, Casado, Priscila Ladeira, Kawase, Tomoyuki, Mourão, Carlos Fernando, and Alves, Gutemberg Gomes

Subjects
PLATELET-derived growth factor, VASCULAR endothelial growth factors, GROWTH factors, BONE growth, CLINICAL medicine, PLATELET-rich fibrin
Abstract

The development of effective biomaterials for tissue regeneration has led to the exploration of blood derivatives such as leucocyte- and platelet-rich fibrin (L-PRF). A novel variant, Albumin-Enriched Platelet-Rich Fibrin (Alb-PRF), has been introduced to improve structural stability and bioactivity, making it a promising candidate for bone regeneration. This study aimed to evaluate Alb-PRF's capacity for cytokine and growth factor release, along with its effects on the proliferation, differentiation, and mineralization of human osteoblasts in vitro. Alb-PRF membranes were analyzed using histological, scanning electron microscopy, and fluorescence microscopy techniques. Cytokine and growth factor release was quantified over seven days, and osteoinductive potential was evaluated with MG-63 osteoblast-like cells. Structural analysis showed Alb-PRF as a biphasic, highly cellularized material that releases lower levels of inflammatory cytokines and higher concentrations of platelet-derived growth factor (PDGF) and vascular endothelial growth factor (VEGF) compared to L-PRF. Alb-PRF exhibited higher early alkaline phosphatase activity and in vitro mineralization (p < 0.05) and significantly increased the OPG/RANKL mRNA ratio (p < 0.05). These results indicate that Alb-PRF has promising potential as a scaffold for bone repair, warranting further in vivo and clinical assessments to confirm its suitability for clinical applications. [ABSTRACT FROM AUTHOR]

Published
2024
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3. In Vitro Evaluation of Cellular Interactions with Nanostructured Spheres of Alginate and Zinc-Substituted Carbonated Hydroxyapatite.

Author

Dornelas, Jessica, Dornelas, Gisele, Tude, Elena Mavropoulos Oliveira, Mourão, Carlos Fernando, Rossi, Alexandre da Malta, and Alves, Gutemberg Gomes

Subjects
FOURIER transform infrared spectroscopy, CARBON analysis, TISSUE engineering, X-ray spectroscopy, SURFACE analysis, BONE regeneration
Abstract

The increasing demand for effective bone regeneration materials drives the exploration of biomaterials with enhanced bioactivity and biocompatibility, such as zinc-substituted compounds. This study investigates the in vitro cellular interactions with nanostructured spheres composed of alginate/carbonated hydroxyapatite (CHA), compared to zinc-substituted CHA (ZnCHA). This work aimed to compare the physicochemical properties and biological effects of ZnCHA and CHA on osteoblasts. ZnCHA was synthesized using a wet chemical method, followed by characterization through X-ray diffraction, Fourier transform infrared spectroscopy, total organic carbon analysis, Wavelength-dispersive X-ray spectroscopy, and BET surface area analysis to assess ion release and structural changes. Biological evaluation was conducted using cell viability, proliferation, and biomineralization assays on osteoblasts. Results showed successful incorporation of zinc and carbonate, leading to reduced crystallinity and increased surface area. Cell viability and proliferation assays indicated ZnCHA's cytocompatibility and enhanced osteoblastic activity, with increased mineralization nodules compared to CHA samples. The study concludes that ZnCHA composites are promising candidates for bone tissue engineering, demonstrating improved cytocompatibility and potential for further preclinical evaluations. [ABSTRACT FROM AUTHOR]

Published
2024
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4. The Incorporation of Zinc into Hydroxyapatite and Its Influence on the Cellular Response to Biomaterials: A Systematic Review.

Author

Dornelas, Jessica, Dornelas, Giselle, Rossi, Alexandre, Piattelli, Adriano, Di Pietro, Natalia, Romasco, Tea, Mourão, Carlos Fernando, and Alves, Gutemberg Gomes

Subjects
BIOABSORBABLE implants, PLASMA spraying, BONE cells, BONE metabolism, BIOMEDICAL materials, BONE regeneration
Abstract

Zinc is known for its role in enhancing bone metabolism, cell proliferation, and tissue regeneration. Several studies proposed the incorporation of zinc into hydroxyapatite (HA) to produce biomaterials (ZnHA) that stimulate and accelerate bone healing. This systematic review aimed to understand the physicochemical characteristics of zinc-doped HA-based biomaterials and the evidence of their biological effects on osteoblastic cells. A comprehensive literature search was conducted from 2022 to 2024, covering all years of publications, in three databases (Web of Science, PUBMED, Scopus), retrieving 609 entries, with 36 articles included in the analysis according to the selection criteria. The selected studies provided data on the material's physicochemical properties, the methods of zinc incorporation, and the biological effects of ZnHA on bone cells. The production of ZnHA typically involves the wet chemical synthesis of HA and ZnHA precursors, followed by deposition on substrates using processes such as liquid precursor plasma spraying (LPPS). Characterization techniques confirmed the successful incorporation of zinc into the HA lattice. The findings indicated that zinc incorporation into HA at low concentrations is non-cytotoxic and beneficial for bone cells. ZnHA was found to stimulate cell proliferation, adhesion, and the production of osteogenic factors, thereby promoting in vitro mineralization. However, the optimal zinc concentration for the desired effects varied across studies, making it challenging to establish a standardized concentration. ZnHA materials are biocompatible and enhance osteoblast proliferation and differentiation. However, the mechanisms of zinc release and the ideal concentrations for optimal tissue regeneration require further investigation. Standardizing these parameters is essential for the effective clinical application of ZnHA. [ABSTRACT FROM AUTHOR]

Published
2024
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6. The Association of Nanostructured Carbonated Hydroxyapatite with Denatured Albumin and Platelet-Rich Fibrin: Impacts on Growth Factors Release and Osteoblast Behavior

Author

de Lima Barbosa, Renata, primary, Rodrigues Santiago Rocha, Neilane, additional, Stellet Lourenço, Emanuelle, additional, de Souza Lima, Victor Hugo, additional, Mavropoulos, Elena, additional, Mello-Machado, Rafael Coutinho, additional, Spiegel, Carolina, additional, Mourão, Carlos Fernando, additional, and Alves, Gutemberg Gomes, additional

Published
2024
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7. Cytocompatibility of filling pastes by primary teeth root simulating model

Author

Pintor, Andréa Vaz Braga, Queiroz, Luciana Domênico, Sancas, Mariana Coutinho, Brochado, Ana Carolina Batista, Spoladore, Janaína, Fonseca-Gonçalves, Andréa, Fidalgo, Tatiana Kelly S., Freitas-Fernandes, Liana Bastos, Valente, Ana Paula, de Souza, Ivete Pomarico Ribeiro, Primo, Laura Guimarães, and Alves, Gutemberg Gomes

Published
2021
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9. Clinical and Biochemical Evaluation of the Use of Alb-PRF Versus L-PRF in Mandibular Third Molar Extractions: A Split-Mouth Randomized Clinical Trial

Author

Javid, Kayvon, primary, Mourão, Carlos Fernando, additional, Mello-Machado, Rafael Coutinho, additional, Sartoretto, Suelen Cristina, additional, Torres, Madelaine, additional, Stellet Lourenço, Emanuelle, additional, Leite, Paulo Emilio Correa, additional, Granjeiro, José Mauro, additional, Alves, Gutemberg Gomes, additional, and Calasans-Maia, Monica Diuana, additional

Published
2023
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11. Association between genetic polymorphisms in DEFB1 and microRNA202 with caries in two groups of Brazilian children

Author

Oliveira, Daniela Silva Barroso de, Segato, Raquel Assed Bezerra, Oliveira, Sara, Dutra, André Luiz Tannus, Santos, Adriana Stone dos, Praxedes, Ananda Deva Noronha, Belém, Leandro Coelho, Antunes, Livia Azeredo, Lips, Andrea, Nelson-Filho, Paulo, da Silva, Léa Assed Bezerra, Alves, Gutemberg Gomes, Antunes, Leonardo Santos, and Küchler, Erika Calvano

Published
2018
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12. Does the association of blood-derived growth factors to nanostructured carbonated hydroxyapatite contributes to the maxillary sinus floor elevation? A randomized clinical trial

Author

de Almeida Barros Mourão, Carlos Fernando, Lourenço, Emanuelle Stellet, Nascimento, Jhonathan Raphael Barros, Machado, Rafael Coutinho Mello, Rossi, Alexandre Malta, Leite, Paulo Emílio Corrêa, Granjeiro, José Mauro, Alves, Gutemberg Gomes, and Calasans-Maia, Mônica Diuana

Published
2019
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17. Effects of platelet-rich fibrin in the behavior of cells from mineralizing tissues. A scoping review of in vitro evidence

Author

Alves, Gutemberg Gomes, De Almeida Barros Mourão, Carlos Fernando, and De Lima, Renata

Subjects
Medical Sciences, Dentistry, FOS: Clinical medicine, Medicine and Health Sciences
Abstract

A systematic, scoping review investigating the main mechanisms involved in the potential effects of Platelet-rich fibrin (PRF) in bone at the cellular level.

Published
2023
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18. CellEscape: o desenvolvimento de um escape room virtual para divulgação científica sobre aplicações de biotecnologia

Author

Sayde, Mariana Ribeiro, primary, Leão, Darcele Christo, additional, Silva, Maria Luiza Alves da, additional, Lahud, YuriVictor, additional, Felix, Gabriel Gavazzi, additional, Willner, Luiz Claudio, additional, Clua, Esteban W. Gonzalez, additional, Alves, Gutemberg Gomes, additional, and Spiegel, Carolina Nascimento, additional

Published
2022
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20. Métodos alternativos para determinação da potência de veneno e antiveneno botrópico: aplicações e perspectivas para o controle da qualidade

Author

Nundes, Renata Norbert Costa, Araújo, Humberto Pinheiro de, Alves, Gutemberg Gomes, Delgado, Isabella Fernandes, Presgrave, Octavio Augusto França, Moura, Wlamir Corrêa de, and Gonzalez, Marcelo Salabert

Subjects
Antiveneno Botrópico, Quality Control, Veneno Botrópico, Alternative Methods, Bothrops Antivenom, Método alternativo para controle da qualidade para veneno e antiveneno botrópico, Bothrops Venom, Métodos Alternativos, Controle da Qualidade
Abstract

Introduction: Accidents with venomous animals are classified as neglected tropical diseases and are currently the most frequent cause of intoxication in humans in Brazil. The only available treatment is the rapid administration of specific, quality-assured antivenoms. To ensure the efficacy and safety of these products, in vivo potency determination tests for venom and antivenom are performed during the production stages, until final release. Despite several studies on alternative methods to the murine assay, no method has been effectively validated. Objective: To compile alternative methods developed for Bothrops antivenoms, assessing the availability of the methods and the prospects and applications in Bothrops venom and antivenom production and quality control laboratories. Method: A search was conducted in PubMed, BVS, and Scopus databases between November 2021 and June 2022. 89 articles were identified, of which 31 were selected according to the eligibility criteria. Results: We observed in the alternative methods identified a preference of 42.80% of the studies for methodologies that use cell lines as an alternative method to the murine assays, and most of these works (58.30%) opted for a VERO cell line. Conclusions: Due to the diversity of toxins found in each genus of snakes, it is understood that the potency assay for antivenoms should be based on the evaluation and precise quantification of the inhibition of biological activity of venoms. Cytotoxicity assays are widely used and have been accumulating evidence of their suitability as an important alternative tool to the murine assay for quality control for Bothrops venom and antivenom. Introdução: Acidentes com animais peçonhentos são classificados como doenças tropicais negligenciadas e são atualmente a mais frequente causa de intoxicação em humanos no Brasil. O único tratamento disponível é a rápida administração de antivenenos específicos e de qualidade garantida. Para assegurar a eficácia e a segurança desses produtos, são realizados ensaios de determinação da potência in vivo para veneno e antiveneno, desde as etapas de produção até sua liberação final. Apesar dos diversos estudos sobre métodos alternativos ao ensaio murino, nenhum método foi efetivamente validado. Objetivo: Compilar os métodos alternativos desenvolvidos para os antivenenos botrópicos, avaliando sua disponibilidade, perspectivas e aplicações em laboratórios de produção e controle da qualidade. Método: Foi realizada uma busca nas bases PubMed, BVS e Scopus entre novembro de 2021 e junho de 2022. Foram identificados 89 trabalhos, dos quais 31 foram selecionados de acordo com os critérios de elegibilidade. Resultados: Nos métodos alternativos identificados, observamos a preferência de 42,80% dos estudos por metodologias que utilizem linhagens celulares como método alternativo aos ensaios murinos, sendo que a maioria destes trabalhos 58,30% optou pela linhagem celular Vero. Conclusões: Pela diversidade das toxinas encontradas em cada gênero de serpentes, entende-se que é de extrema importância que o ensaio de potência dos antivenenos tenha como base a avaliação e a quantificação precisa da inibição da atividade biológica dos venenos. Ensaios de citotoxicidade são amplamente utilizados e têm acumulado evidências de sua adequação como importante ferramenta alternativa ao ensaio murino para o controle da qualidade de veneno e antiveneno antibotrópico.

Published
2022

23. “Sticky Bone” Preparation Device: A Pilot Study on the Release of Cytokines and Growth Factors

Author

Gheno, Ezio, primary, Alves, Gutemberg Gomes, additional, Ghiretti, Roberto, additional, Mello-Machado, Rafael Coutinho, additional, Signore, Antonio, additional, Lourenço, Emanuelle Stellet, additional, Leite, Paulo Emílio Correa, additional, Mourão, Carlos Fernando de Almeida Barros, additional, Sohn, Dong-Seok, additional, and Calasans-Maia, Mônica Diuana, additional

Published
2022
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24. Characterization and Applicability of a Bone Spheroid Model for the Evaluation of Cytocompatibility of Bone Substitutes.

Author

Brochado, Ana Carolina Batista, Silva, Daniela Costa, Silva, Joice Correa da, Lowenstein, Adam, Gameiro, Vinicius Schott, Mavropoulos, Elena, Mourão, Carlos F., and Alves, Gutemberg Gomes

Subjects
BONE substitutes, MATERIALS testing, CELL culture, CYTOCOMPATIBILITY, OPACITY (Optics), BONE cells, POLYLACTIC acid
Abstract

In vitro cell-based tests are an important preclinical step for the safety assessment of biomaterials and drugs. Three-dimensional cell culture models (3D) may improve the limitations of the usual 2D models, as they better simulate a physiological environment. This work describes the characterization of a 3D spheroid model of MC3T3-E1 murine preosteoblasts for the testing of bone-substitute materials and investigates its adequacy to some of the most employed cell viability tests. The spheroids presented structural stability for 28 days in culture, with a regular spheroidal aspect, compact surface, and dense inner structure, with high potential for mineralization, but a time-dependent reduction in size. The use of colorimetric tests (MTT, XTT, and NRU) did not achieve satisfactory optical densities and did not correlate with cell density in the 3D model, as the aggregates remain strongly stained even after dye extraction steps. On the other hand, the LDH test achieved appropriate optical density and a high correlation with cell density (r2 = 0.77) and identified a dose–response for a well-known cytotoxic polymer (latex), while no toxicity was identified for biocompatible PLA wires. These results indicate that material testing with 3D bone cell models requires a careful choice of test methods and parameters. [ABSTRACT FROM AUTHOR]

Published
2023
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25. Osteosphere Model to Evaluate Cell–Surface Interactions of Implantable Biomaterials

Author

Brochado, Ana Carolina Batista, primary, de Souza, Victor Hugo, additional, Correa, Joice, additional, dos Anjos, Suzana Azevedo, additional, de Almeida Barros Mourão, Carlos Fernando, additional, Cardarelli, Angelo, additional, Montemezzi, Pietro, additional, Gameiro, Vinicius Schott, additional, Pereira, Mariana Rodrigues, additional, Mavropoulos, Elena, additional, and Alves, Gutemberg Gomes, additional

Published
2021
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27. In vivo evaluation of the biocompatibility and biodegradation of a new denatured plasma membrane combined with liquid PRF (Alb-PRF)

Author

Gheno, Ezio, de Almeida Barros Mourão, Carlos Fernando, de Mello-Machado, Rafael Coutinho, Lourenço, Emanuele Stellet, Miron, Richard J, Catarino, Karoline Ferreira Farias, Alves, Adriana Terezinha, Alves, Gutemberg Gomes, and Calasans-Maia, Mônica D.

Subjects
digestive system diseases
Abstract

Guided bone regeneration (GBR) is a process that involves the regeneration of bone defects through the application of occlusive membranes that mechanically exclude the population of non-osteogenic cells from the surrounding soft tissue. Interestingly, platelet-rich fibrin (PRF) has previously been proposed as an autologous GBR membrane despite its short-term resorption period of 2–3 weeks. Recent clinical observations have demonstrated that, by heating a liquid platelet-poor plasma (PPP) layer and mixing the cell-rich buffy coat zone, the resorption properties of heated albumin gel with liquid-PRF (Alb-PRF) can be significantly improved. The aim of this study was to evaluate the inflammatory reaction, biocompatibility, and extended degradation properties of a new autologous Alb-PRF membrane in comparison to commonly utilized standard PRF after nude mice implantation, according to ISO 10993–6/2016. Two standard preparations of PRF (L-PRF and H-PRF) were compared to novel Alb-PRF following subcutaneous implantation at 7, 14, and 21 days. All groups demonstrated excellent biocompatibility owing to their autologous sources. However, it is worth noting that, while both L-PRF and H-PRF membranes demonstrated significant or complete resorption by 21 days, the Alb-PRF membrane remained volume-stable throughout the duration of the study. This study demonstrates—for the first time, to the best of our knowledge—a marked improvement in the membrane stability of Alb-PRF. This indicates its future potential for use as a biological barrier membrane for GBR procedures with a long-lasting half-life, or as a biological filler material in esthetic medicine applications. Thus, further studies are warranted to explore future clinical applications in various fields of medicine.

Published
2021
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30. Effects of Leukocyte-Platelet-Rich Fibrin (L–PRF) on Pain, Soft Tissue Healing, Growth Factors, and Cytokines after Third Molar Extraction: A Randomized, Split-Mouth, Double-Blinded Clinical Trial

Author

da Silva, Madelaine Torres, primary, de Almeida Barros Mourão, Carlos Fernando, additional, Mello-Machado, Rafael Coutinho, additional, Montemezzi, Pietro, additional, de Lima Barbosa, Renata, additional, Sartoretto, Suelen Cristina, additional, Leite, Paulo Emílio Correa, additional, Javid, Kayvon, additional, Kawase, Tomoyuki, additional, Alves, Gutemberg Gomes, additional, and Calasans-Maia, Mônica Diuana, additional

Published
2021
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34. Cytocompatibility of filling pastes by primary teeth root simulating model

Author

Pintor, Andréa Vaz Braga, primary, Queiroz, Luciana Domênico, additional, Sancas, Mariana Coutinho, additional, Brochado, Ana Carolina Batista, additional, Spoladore, Janaína, additional, Fonseca-Gonçalves, Andréa, additional, Fidalgo, Tatiana Kelly S., additional, Freitas-Fernandes, Liana Bastos, additional, Valente, Ana Paula, additional, de Souza, Ivete Pomarico Ribeiro, additional, Primo, Laura Guimarães, additional, and Alves, Gutemberg Gomes, additional

Published
2020
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37. In vivo evaluation of the biocompatibility and biodegradation of a new denatured plasma membrane combined with liquid PRF (Alb-PRF)

Author

Gheno, Ezio, primary, Mourão, Carlos Fernando de Almeida Barros, additional, Mello-Machado, Rafael Coutinho de, additional, Stellet Lourenço, Emanuele, additional, Miron, Richard J, additional, Catarino, Karoline Ferreira Farias, additional, Alves, Adriana Terezinha, additional, Alves, Gutemberg Gomes, additional, and Calasans-Maia, Mônica D., additional

Published
2020
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38. Métodos alternativos para a detecção de pirogênios em produtos e ambientes sujeitos a Vigilância Sanitária: avanços e perspectivas no Brasil a partir do reconhecimento internacional do Teste de Ativação de Monócitos

Author

da Silva, Cristiane Caldeira, de Oliveira, Carolina Barbara Nogueira, Carneiro, Patrícia dos Santos, Marengo, Eliana Blini, de Mattos, Katherine Antunes, de Almeida, Ricardo Sergio Couto, Spoladore, Janaína, Alves, Gutemberg Gomes, Presgrave, Octavio Augusto França, and Delgado, Isabella Fernandes

Subjects
Quality Control, Teste de Ativação de Monócitos, Técnicas in vitro, Legislação, Alternative Methods, Legislation, Pyrogen, Monocyte Activation Test, Pirogênio, Métodos Alternativos, Controle da Qualidade, In vitro Techniques
Abstract

Introduction: The detection of pyrogens is essential for the quality control of injectable products. The Rabbit Pyrogen Test remains widely used, despite the existence of alternative methods such as the Monocyte Activation Test (MAT). Objective: To review the use of alternative methods for pyrogen testing, pointing out advances and perspectives from the recognition of MAT by the European pharmacopoeia and its acceptance for regulatory purposes in Brazil. Method: A search was performed on the PubMed and BVS databases, with further classification, categorization by topic and critical analysis of the results. Results: Twenty-four papers were identified, addressing topics such as applications of MAT, its validation and comparisons with in vivo tests. MAT presented better results when compared to other tests, both in the evaluation of biological products and in the detection of non-endotoxin pyrogens. Limitations to diffusion include difficulties in obtaining whole human blood as a source of monocytes, for which several alternatives have been proposed. Conclusions: MAT is a promising method, with application in safety evaluation of new technologies. Its application in Brazil depends on a national implementation policy, which might include greater integration between BraCVAM, Concea and RENAMA in search for its recognition for regulatory purposes. Introdução: A detecção de pirogênios é imprescindível no controle da qualidade de produtos injetáveis. O Teste de Pirogênio em coelhos ainda tem larga aplicação, apesar da existência de métodos alternativos como o Teste de Ativação de Monócitos (MAT). Objetivo: Revisar o uso dos métodos alternativos no teste de pirogênio, apontando avanços e perspectivas a partir do reconhecimento do MAT pela Farmacopeia Europeia e sua aceitação para fins regulatórios no Brasil. Método: Uma busca foi realizada nas bases PubMed e BVS, com posterior classificação, categorização por assuntos e análise crítica dos resultados. Resultados: Foram identificados 24 trabalhos, abordando temas como as aplicações do MAT, sua validação e comparação com testes in vivo. O MAT apresentou melhores resultados quando comparado a outros testes, tanto na avaliação de produtos biológicos como na detecção de pirogênios não-endotoxinas. Limitações para sua difusão incluem a dificuldade de obtenção de sangue total humano como fonte de monócitos, para o qual diversas alternativas têm sido propostas. Conclusões: O MAT se mostra um método promissor, com aplicação na avaliação da segurança de novas tecnologias. Sua aplicação no Brasil depende de uma política nacional de implantação, que inclua maior Integração entre BraCVAM, Concea e RENAMA na busca por seu reconhecimento para fins regulatórios.

Published
2018

42. Effects of rotor angle and time after centrifugation on the biological in vitro properties of platelet rich fibrin membranes.

Author

Lourenço, Emanuelle Stellet, Alves, Gutemberg Gomes, Lima Barbosa, Renata, Spiegel, Carolina Nascimento, Mello‐Machado, Rafael Coutinho, Al‐Maawi, Sarah, Ghanaati, Shahram, and Almeida Barros Mourão, Carlos Fernando

Subjects
CENTRIFUGATION, PLATELET-rich fibrin, BLOOD cell count, ERYTHROCYTES, BLOOD platelets
Abstract

This study evaluated the impact of rotor angle and time of storage after centrifugation on the in vitro biological properties of platelet‐rich fibrin (PRF) membranes. Blood samples (n = 9) were processed with a vertical fixed‐angle (V) or a swing‐out horizontal (H) centrifuge, with 20–60 min of sample storage after centrifugation. Leukocytes, platelets, and red blood cells were counted, and fibrin architecture was observed by scanning electron microscopy (SEM). The release of FGF2, PDGFbb, VEGF, IL‐6, and IL‐1β was measured after incubation on culture media for 7–21 days. Cell content was equivalent in all experimental groups (p >.05). The fibrin matrix was similar for fixed‐angle and horizontal centrifugation. Horizontal centrifugation induced a twofold increase in PDGF and 1.7× increase on FGF release as compared to V samples, while IL‐1β was significantly reduced (p <.05). No significant difference was observed on the release of growth factors and cytokines at different times after centrifugation (p <.05). These data suggest that both angles of centrifugation produce PRF membranes with similar structure and cellularity, but horizontal centrifugation induces a higher release of growth factors. Higher times of storage after centrifugation did not impact on cell content and the release of growth factors. [ABSTRACT FROM AUTHOR]

Published
2021
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43. A Proof of the Low Speed Centrifugation Concept in Rodents: New Perspectives forIn VivoResearch

Author

Ghanaati, Shahram, primary, Al-Maawi, Sarah, additional, Herrera-Vizcaino, Carlos, additional, Alves, Gutemberg Gomes, additional, Calasans-Maia, Mônica Diuana, additional, Sader, Robert, additional, Kirkpatrick, C. James, additional, Choukroun, Joseph, additional, Bonig, Halvard, additional, and Mourão, Carlos Fernando de Almeida Barros, additional

Published
2018
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45. Does the association of blood-derived growth factors to nanostructured carbonated hydroxyapatite contributes to the maxillary sinus floor elevation? A randomized clinical trial

Author

de Almeida Barros Mourão, Carlos Fernando, primary, Lourenço, Emanuelle Stellet, additional, Nascimento, Jhonathan Raphael Barros, additional, Machado, Rafael Coutinho Mello, additional, Rossi, Alexandre Malta, additional, Leite, Paulo Emílio Corrêa, additional, Granjeiro, José Mauro, additional, Alves, Gutemberg Gomes, additional, and Calasans-Maia, Mônica Diuana, additional

Published
2018
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50. Genetic Polymorphisms in DEFB1 and miRNA202 Are Involved in Salivary Human β-Defensin 1 Levels and Caries Experience in Children

Author

Lips, Andrea, primary, Antunes, Leonardo Santos, additional, Antunes, Lívia Azeredo, additional, Abreu, Júlia Guimarães Barcellos de, additional, Barreiros, Driely, additional, Oliveira, Daniela Silva Barroso de, additional, Batista, Ana Carolina, additional, Nelson-Filho, Paulo, additional, Silva, Léa Assed Bezerra da, additional, Silva, Raquel Assed Bezerra da, additional, Alves, Gutemberg Gomes, additional, and Küchler, Erika Calvano, additional

Published
2017
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