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Meat is an important part of the food pyramid in Mexico, to such an extent that it is included in the basic food basket. In recent years, there has been great interest in the application of so-called emerging technologies, such as high-intensity ultrasound (HIU), to modify the characteristics of meat and meat products. The advantages of the HIU in meat such as pH, increased water-holding capacity, and antimicrobial activity are well documented and conclusive. However, in terms of meat tenderization, the results are confusing and contradictory, mainly when they focus on three HIU parameters: acoustic intensity, frequency, and application time. This study explores via a texturometer the effect of HIU-generated acoustic cavitation and ultrasonoporation in beef (m. Longissimus dorsi). Loin-steak was ultrasonicated with the following parameters: time tHIU = 30 min/each side; frequency fHIU = 37 kHz; acoustic intensity IHIU = ~6, 7, 16, 28, and 90 W/cm2. The results showed that acoustic cavitation has a chaotic effect on the loin-steak surface and thickness of the rib-eye due to Bjerknes force, generating shear stress waves, and acoustic radiation transmittance via the internal structure of the meat and the modification of the myofibrils, in addition to the collateral effect in which the collagen and pH generated ultrasonoporation. This means that HIU can be beneficial for the tenderization of meat.

En años actuales y atendiendo las necesidades de los consumidores, se ha incrementado el consumo de alimentos funcionales. Dentro de estos alimentos se encuentran los alimentos que contienen prebióticos y probióticos. Sin embargo, actualmente se han incluido dos términos nuevos, paraprobióticos y postbióticos: los primeros son células microbianas inactivas o no viables, mientras que los postbióticos son factores solubles o metabolitos que son secretados por las bacterias vivas o bien que son liberados después de una lisis celular. Dependiendo de donde se producen los postbióticos se clasifican en metabolitos microbianos (enzimas, lípidos, ácidos orgánicos, polisacáridos y péptidos/proteína) y componentes microbianos (proteínas de superficie celular, ácido lipotéicoico, peptidoglucano, polisacáridos y ácido teicoico). La absorción de estos compuestos se da en las células del intestino, que es donde ejercen su función. Actualmente se emplea el ultrasonido de alta intensidad (UAI) como una herramienta para la obtención de estos compuestos, debido a que este produce un fenómeno llamado cavitación acústica, el cual genera el crecimiento e implosión de burbujas de vapor, generando la formación de poros, también llamada ultrasonoporación, de manera transitoria en la membrana celular, permitiendo con ello el intercambio y liberación de moléculas o postbióticos. DOI: https://doi.org/10.54167/tecnociencia.v15i2.836

Vitamin B12 and melatonin were added to whole milk-stirred yogurt to evaluate its physicochemical (fat, protein, total solids, moisture, syneresis, and color), rheological (flow behavior and dynamic rheology), and color characteristics as L , a ∗ , and b ∗ and color change ( ∆ E ). For this, five treatments (T) were elaborated as follows: T1 = yogurt (Y) without vitamin B12 and melatonin, T2 = Y with 3 μg/L of B12, T3 = Y with 5 mg/L of melatonin, T4 = Y with 2 μg/L of B12 and 3 mg/L of melatonin, and T5 = Y with 3 μg/L of B12 and 5 mg/L of melatonin. The fortification of yogurt with vitamin B12 and melatonin modified ( P < 0.05 ) the content of fat, moisture, and syneresis. Treatment 5 presented the lowest content of fat and the largest content of moisture ( P < 0.05 ). Treatment 4 had the largest syneresis at day 1, and at day 14, there was not differences in syneresis among treatments ( P > 0.05 ). Regarding color, luminosity ( L ∗ ) did not have significant differences ( P > 0.05 ) among treatments and neither among storage days, except for T5 that presented the lowest value at day 14. Regarding parameter a ∗ , all treatments presented negative values (tendency to green), and T2, T3, and T5 showed significant differences ( P < 0.05 ) trough time. No significant differences ( P > 0.05 ) were observed for the b ∗ values neither among treatments nor among time. The highest value in Δ E , compare to the control, was observed in T5. Regarding flow behavior, T1 showed a significantly different ( P < 0.05 ) higher flow behavior ( n ) than the rest of the treatments. In general, all the samples indicate an increase ( P < 0.05 ) in the flow behavior ( n ) through the storage time. And the consistency index ( K ) showed significant difference ( P < 0.05 ) between T1 and T2 and T3 and T4. In conclusion, the addition of vitamin B12 and melatonin to yogurt generates changes in the physicochemical and rheological properties; likewise, the addition of melatonin in combination with vitamin B12 showed that the viscoelastic characteristics of the fortified yogurts are like the control yogurt. Since these, treatment 5 presented the most similar values of rheology and syneresis compared to the control, so this combination of B12 and melatonin could be employed to develop a new functional yogurt.

In recent decades, food research has focused on hybrid systems, that is, the application of nanomaterials and the so-called emerging technologies, whose objective is to increase the quality of food. Among these technologies that are different from thermal is high intensity ultrasound. This chapter presents and describes the interaction of acoustic waves supported by classical physics and nanomaterials generated by nanotechnology carried out in contemporary physics, all integrated as a multidisciplinary knowledge applied to food. Acoustic waves have a spectrum called ultrasound, with an approximate frequency range between 20 kHz and 1 GHz, and this is divided into low-intensity ultrasound (LIU), whose objective is to characterize materials. On the contrary, high-intensity ultrasound (HIU) has the objective of carrying out destructive tests, modifying the study samples. This happens because the HIU generates a phenomenon called acoustic cavitation, which consists of the generation, growth, and implosion of microbubbles, causing alterations in the near and far acoustic field. The proposed review focuses on the application of high-intensity ultrasound to be used in the food industry. Subsequently, a brief approach is made to nanotechnology and nanomaterials and how they have been incorporated into the food industry.

The population of the Papaloapan region consume artisan fresh cheeses and no pathogen outbreaks have been reported recently. The microbiota is responsible to develop desirable characteristics of cheeses and undesirable characteristics due to the presence of certain pathogens microorganisms. Therefore, to identify the microorganisms of fresh cheeses is an important issue for the producers, consumers, and authorities. 11 Artisan fresh cheese samples from the Papaloapan region were collected in the summer and 11 samples in winter to characterize their microbiota. Traditional microbial techniques were used to identify the fungus and the amplification of the 16S rRNA gene and PCR-denaturing gradient gel electrophoresis (DGGE) The population of the Papaloapan region consume artisan fresh cheeses and no pathogen outbreaks have been reported recently. The microbiota is responsible to develop desirable characteristics of cheeses and undesirable characteristics due to the presence of certain pathogens microorganisms. Therefore, to identify the microorganisms of fresh cheeses is an important issue for the producers, consumers, and authorities. 11 Artisan fresh cheese samples from the Papaloapan region were collected in the summer and 11 samples in winter to characterize their microbiota. Traditional microbial techniques were used to identify the fungus and the amplification of the 16S rRNA gene and PCR-denaturing gradient gel electrophoresis (DGGE) The population of the Papaloapan region consume artisan fresh cheeses and no pathogen outbreaks have been reported recently. The microbiota is responsible to develop desirable characteristics of cheeses and undesirable characteristics due to the presence of certain pathogens microorganisms. Therefore, to identify the microorganisms of fresh cheeses is an important issue for the producers, consumers, and authorities. 11 Artisan fresh cheese samples from the Papaloapan region were collected in the summer and 11 samples in winter to characterize their microbiota. Traditional microbial techniques were used to identify the fungus and the amplification of the 16S rRNA gene and PCR-denaturing gradient gel electrophoresis (DGGE) method was used for bacteria identification. For all the samples, the presence of aerobic mesophiles, Streptococcus mesophiles and thermophiles, Lactobacillus mesophiles, Leuconostoc, total coliforms, Staphylococcus aureus, molds, and yeasts were identified. The complexity and variety of microorganisms in the summer and winter seasons samples were not significantly different. In conclusion, all samples of fresh artisan cheeses were under high microbial loads. Lactic Acid Bacteria (LAB) were in a typical load, as established by the quality and safety standards in the food industry. Conversely, pathogenic bacteria exceeded this limit. The microorganisms present in the fresh artisanal cheeses of the Papaloapan region were identified with precision, regarding the count and their diversity. A recommendation for the cheese manufacturers is to prepare starter cultures by selecting the appropriate microorganisms to produce the desirable characteristics such as aroma and flavor and reduce the risk of microbial infections by using pasteurized milk.

Se evaluó el efecto del aceite esencial de orégano (AEO) y bagazo de orégano (BO) incorporados en la dieta sobre los parámetros productivos, variables de sacrificio y calidad de la carne de conejos. Un total de 100 conejos (30 d de edad) fueron distribuidos en seis tratamientos T1: control, T2: 0.25 g/kg de AEO, T3: 0.40 g/kg de AEO, T4: 20% de BO, T5: 0.25 g/kg de AEO + 20% de BO y T6: 0.40 g / kg de AE + 20% de BO. El mayor peso vivo fue el T6 (P˂0.0001). En 37, 44 y 51 días, T3 presentó el menor consumo de alimento (P=0.0089) y T6 tuvo la mejor ganancia de peso (P=0.0172). La conversión alimenticia fue mejor (P=0.0138) en T5 a los 37 días. El rendimiento de la canal fría y lomo fue más alto en T2, T4 y T5 (P

Raspberries are widely consumed; the taste of the fruit is determined by the interaction between sugars, organic acids and a set of volatile compounds. Meanwhile, organic agriculture has developed rapidly as an alternative to conventional system and has been driven by the demand for pesticide-free food that brings greater benefits to human health. However, this system can alter the integral quality of the fruit, including the aroma, which has been little studied. Therefore, the objective of this study was to evaluate the effect of the application of organic and conventional fertilizers, in the presence of volatile compounds synthesized in the cv. ‘Heritage’ raspberry, during two crop cycles. The qualitative profile of volatile compounds was analysed by mass gas chromatography, obtaining as a result that fertilization treatments significantly affected the emission of volatile compounds in the most abundant functional group (C13 norisoprenoids) 48.5 vs. 25.8% and 56.9 vs. 29.1% in conventional and organic, in 2017 and 2018, respectively (p ≤ 0.05). The crop cycle affected the concentration of aldehydes (11.2 vs. 30.6% in organic, in 2017 and 2018, respectively (p ≤ 0.05). Impact compounds such as α-ionone (13.92 vs. 9.08% and 25.34 vs. 9.17% in conventional and organic in 2017 and 2018, respectively) and β-ionone (24.93 vs. 14.10% and 22.66 vs. 15.94% in conventional and organic in 2017 and 2018, respectively), presented greater abundance in conventional fertilization (p ≤ 0.05). Since there were changes in the volatile compounds, it is recommended to study whether the consumer perceives these changes.

Fermented whey-based beverages were elaborated using commercial probiotics cultures (CHR HANSEN): BCT-1®, ABT-4®, ABC where A: Lactobacillus acidophilus, B: Bifidobacterium animalis subsp. lactis, C: Lactobacillus paracasei subsp. paracasei and T: Streptococccus thermophiles. Three treatments were prepared with these probiotics cultures and a treatment without cultures added as a control. All beverage were maintained at 4 °C and evaluated during their shelf life at 0, 7, 14 and 21 days. The variables analyzed were peptide concentration, proteolysis, ACE-inhibitory activity and, peptide and aromatic amino acid profiles. Principal component analysis (PCA) was conducted to assess the relationship between these variables. All whey-based fermented beverages added with probiotics presented ACE inhibitory activity (22–100%). However, whey-based beverage without probiotics added had the highest ACE inhibitory activity (100%), and it was maintained during its shelf life. Fermented beverage with ABT-4® culture had an ACE inhibitory activity from 80 to 100% during its shelf life. Thus can be attributed to the release of peptides during processing. Meanwhile, significative correlations were observed between variables varying this depending on the treatment, both, in magnitude and in direct or inverse direction (0.684–0.986). The ACE inhibitory activity was correlated to proteolysis and peptide and aromatic amino acids profiles. And, the IC(50) was observed in the range of 0.01–0.055 mg/mL.

Beet has been used as an ingredient for functional foods due to its high antioxidant activity, thanks to the betalains it contains. The effects of the addition of beet extract (liquid and lyophilized) on the physicochemical characteristics, color, antioxidant activity (AA), total betalains (TB), total polyphenols (TP), and total protein concentration (TPC) were evaluated on stirred yogurt. The treatments (T1-yogurt natural, T2-yogurt added with beet juice, T3-added extract of beet encapsulated with maltodextrin, and T4-yogurt added with extract of beet encapsulated with inulin) exhibited results with significant differences (p &lt, 0.05). The highest TB content was observed in T2 (209.49 ± 14.91), followed by T3 (18.65 ± 1.01) and later T4 (12.96 ± 0.55). The highest AA was observed on T2 after 14 days (ABTS˙ 0.819 mM TE/100 g and DPPH˙ 0.343 mM TE/100 g), and the lowest was found on T1 at day 14 (ABTS˙ 0.526 mM TE/100 g and DPPH˙ 0.094 mM TE/100 g). A high content of TP was observed (7.13 to 9.79 mg GAE/g. The TPC varied between 11.38 to 12.56 µg/mL. The addition of beet extract significantly increased AA in yogurt, betalains being the main compounds responsible for that bioactivity.

Microbial quality and the prevalence of foodborne pathogens (E. coli 0157:H7, Salmonella spp., Listeria monocytogenes and Campylobacter jejuni) of cheeses sold at three retail points (supermarkets [SM], street markets [ST], and convenience grocery stores [CGS]) in Chihuahua, Mexico, were evaluated (n=90). The most commonly found cheeses in the retail points were Chihuahua (60%) and Ranchero (28.8%). According to Mexican standards, three SM, two CGS, and none of the ST cheeses complied with the regulations. Two cheeses (cheddar and Ranchero) from SM (2.22%) were positive for L. monocytogenes. Eight cheeses (8.88%) were positive for Salmonella spp. (5 from ST and 3 from CGS). E.coli 0157:H7 and Campylobacter jejuni were negative in all samples. In the Ranchero cheese, total coliforms (TC), faecal coliforms (FC), and yeast and moulds (Y&M) showed no significant differences (P > 0.05) among retail points. Nevertheless, in Chihuahua cheese, the numbers of total coliforms, faecal coliforms, and yeast and moulds were statistically different (P < 0.05) among retail points. PSM Chihuahua cheese had the lowest numbers of total (0.99 ± 1.0 Log10CFU/g) and faecal coliforms (166 ± 154 Log10CFU/g). CGS cheeses had the lowest counts in terms of yeast and moulds (3.1 ± 2.2 Log10CFU/g). The results revealed that most cheeses, regardless of the retail point, did not conform to Mexican standards. The number of total and faecal coliforms indicates either flaws during the production and commercialization processes or the ill-handling of raw materials.

Background The production of agricultural wastes still growing as a consequence of the population growing. However, the majority of these residues are under-utilized due their chemical composition, which is mainly composed by cellulose. Actually, the search of cellulases with high efficiency to degrade this carbohydrate remains as the challenge. In the present experiment, two genes encoding an endoglucanase (EC 3.2.1.4) and β-glucosidase (EC 3.2.1.21) were overexpressed in Escherichia coli and their recombinant enzymes (egl-FZYE and cel-FZYE, respectively) characterized. Those genes were found in Trabulsiella odontermitis which was isolated from the gut of termite Heterotermes sp. Additionally, the capability to release sugars from agricultural wastes was evaluated in both enzymes, alone and in combination. Results The results have shown that optimal pH was 6.0 and 6.5, reaching an activity of 1051.65 ± 47.78 and 607.80 ± 10.19 U/mg at 39 °C, for egl-FZYE and cel-FZYE, respectively. The Km and Vmax for egl-FZYE using CMC as substrate were 11.25 mg/mL and 3921.57 U/mg, respectively, whereas using Avicel were 15.39 mg/mL and 2314.81 U/mg, respectively. The Km and Vmax for cel-FZYE using Avicel as substrate were 11.49 mg/mL and 2105.26 U/mg, respectively, whereas using CMC the enzyme did not had activity. Both enzymes had effect on agricultural wastes, and their effect was improved when they were combined reaching an activity of 955.1 ± 116.1, 4016.8 ± 332 and 1124.2 ± 241 U/mg on corn stover, sorghum stover and pine sawdust, respectively. Conclusions Both enzymes were capable of degrading agricultural wastes, and their effectiveness was improved up to 60% of glucose released when combined. In summary, the results of the study demonstrate that the recombinant enzymes exhibit characteristics that indicate their value as potential feed additives and that the enzymes could be used to enhance the degradation of cellulose in the poor-quality forage generally used in ruminant feedstuffs.

Opuntia ssp. is the main genus of the Cactaceae family that includes about 1500 species in the world. Opuntia ssp. edible parts include cladodes and their fruits, and their by-products are rich in dietary fiber, minerals, and bioactive compounds. These ingredients are currently being investigated for their application in edible films and coatings. Edible films and coatings are defined as continuous matrices of polymeric material. Among various functions and specific characteristics of edible films or coatings are; suitable water barrier efficiency, control of the gas exchange, retard solute, oil, and fat migration. Edible films and coatings research from Opuntia polysaccharides and its derivates are an exciting and novelty area that has been developed in recent years. This chapter aims to evaluate the state of Opuntia’s art as a source of polymers to incorporate as edible films and coatings.

Graphical abstract, Highlights • High-intensity ultrasound (HIU) before freezing produced intense and bright yellow-orange tones. • The post-freezing application of HIU produced pale red tones with little brightness. • HIU before freezing accelerates rigor mortis resolution. • HIU before freezing improve the tenderness of rabbit meat. • HIU after freezing decreased the WHC and yield (weigh loss) of the meat., High intensity ultrasound (HIU) is a technique with the potential to improve meat quality, however, more research is needed on its application within the chain of cold storage and freezing. This study evaluates the effect of HIU (40 kHz, 9.6 W/cm2, 20 and 40 min) and post-mortem development on the yield and physicochemical quality of rabbit meat in samples treated with HIU pre- and post-storage in a freezer (120 h at −20 °C). Twenty rabbit carcasses were vacuum packed 12 h post-mortem, placed in a fridge at 4 °C for 24 h, and divided in two groups (HIU application before or after freezing), before assigning the treatments. The results show that HIU before freezing produced intense and bright orange-yellow colours, whereas its application after freezing resulted in pale red tones. HIU application accelerates rigor mortis resolution when it is applied before freezing and causes a significant decrease in pH immediately following the HIU treatment. Post-freezing application of HIU is not recommended because it considerably increased weight loss and toughening of the meat when long exposure times were used (40 min). In contrast, a short treatment duration with HIU mitigated the effects of freezing and produced significant increases in water-holding capacity (WHC) after cold storage. The yield (weight loss) of the rabbit meat was not affected when HIU was applied pre-freezing. The application of HIU pre-freezing constitutes a promising technology because it increased the tenderness and the WHC of rabbit meat. However, more research is needed to improve the appearance before scaling up to industrial levels.

Background: Ready to eat sliced-cooked-meat-products (RTE) are popular convenience foods. Slicing of such products at retail point is a common practice in supermarkets. Due to handling, as well as the supermarket environment, it has been suggested that the counts and presence of specific lactic acid bacteria (LAB) in these products may be associated with their rapid deterioration and short shelf life. Objectives: The aim was to quantify and identify LAB in commercial sliced cooked ham. Methods: Thirty samples of sliced cooked ham were collected from five supermarkets. Each sample was analyzed in terms of: Lactobacillus on De Man Rogosa, Sharpe agar (30(C), mesophilic (30(C) and thermophilic (37ºC ) Streptococcus on M17 agar containing 1% lactose, Lactococcus on LM17 agar containing 100 (g per liter of cycloheximide (25(C) and Enterococci on Kanamicin Aesculin Azide agar containing kanamycin (37(C), all under anaerobic conditions (Gas-Pack System(, BBL) for 48-72h. Twenty-one colonies were randomly picked and physiologically and biochemically characterized. Results: No difference was observed in genuses between supermarkets; however, significant differences were observed between microbial genuses. Enterococci showed the lowest count (2.34 ± 0.05 log10 CFU/g) and Lactobacilli had the highest counts (5.98 ± 0.04 log10 CFU/g). Of the strains isolated 23.8% were identified as thermophillic Lactobacillus, 23.8% mesophilic Lactobacillus, 28.6% Enterococcus, 14.3% Lactococcus and 9.5% Streptococcus. From these, only six could be characterized to species level; one was L.lactis subsp. lactis and five were L. amylolyticus. Conclusions: High LAB counts are a common cause of spoilage in RTE meat products, since these are commonly found in meat environments. Therefore a study on the processing, distribution and in-site handling of RTE meat products at supermarkets may be necessary in order to increase its shelf life.

The aim of this work is to characterize the natural microflora of artisanal Ranchero cheese and to identify Lactococcus isolates. Ten artisanal Ranchero cheese samples made with raw milk were obtained from local producers located in different geographical areas. Aerobic mesophilic, Staphylococci, Psychrophilic , total coliforms, molds, yeast and, lactic acid bacteria (LAB) (Lactococcus , Streptococcus mesophilic and thermophilic, Lactobacillus , Enterococcus, Leuconostoc) were numbered through agar plating. LAB isolates were classified by genus. Then, nineteen randomly selected presumptive Lactococcus isolates were assigned to specie by PCR amplification and DNA sequence. A high number of aerobic mesophilic Staphylococci, Psychrophilic, total coliforms, molds, and yeast were found. Lactococci, mesophilic and thermophilic Streptococci and Lactobacilli were the dominant LAB. Enterococci and Leuconostoc were also present. The isolates were identified as L. lactis subsp. lactis and L. garvieae . And both were found to be the dominant Lactococcus species that could be selected and used in a starter culture. Sanitation deficiencies in the production of artisanal Ranchero cheese were evident, which may translate into being a potential consumers health risk factor.

Resumen Se evaluó el efecto de la inclusión de aceite esencial de orégano (AEO) en el agua de bebida sobre la microbiología intestinal, características fisicoquímicas y rendimiento en canal y carne de 440 pollos de engorda de 1 d de edad no sexados (42.59 ± 1.63 g de peso) y alimentados durante 42 d. Las aves fueron asignadas al azar en cuatro tratamientos con 11 repeticiones de 10 aves con 0, 100, 200 y 400 p.p.m. de AEO en el agua de bebida. Se aplicó a los datos un análisis de varianza (ANDEVA) y un análisis multivariado (MANDEVA). No se encontraron diferencias significativas (P>0.05) en las variables microbiológicas, fisicoquímicas ni rendimiento en canal. Se concluye que el AEO en las concentraciones utilizadas no redujo significativamente los conteos de Salmonella spp. y Campylobacter spp. en ciego, no afectó las propiedades fisicoquímicas de la carne ni de rendimiento de canal.

El rumen es un ecosistema complejo donde los nutrientes consumidos por los rumiantes son digeridos mediante un proceso de ferme ntacion realizado por los microorganismos ruminales (bacterias, protozoos y hongos). Dichos microorganismos estan en simbiosis, debido a su capacidad de adaptacion e interaccion, y mientras el rumiante proporciona el ambiente necesario para su establecimiento estos proporcionan energia al animal, la que proviene de los productos finales de la fermentacion. Dentro del rumen, los microorganismos coexisten en un entorno reducido y a un pH cercano a la neutralidad. Estos microorganismos fermentan los sustratos presentes en la dieta del rumiante (azucares, proteinas y lipidos). Sin embargo, el proceso de fermentacion no es 100% eficaz, ya que durante la fermentacion existen perdidas de energia, principalmente en forma de gas metano (CH 4 ), el que representa un problema medioambiental, ya que es un gas de efecto invernadero. Por consiguiente, para mejorar la eficiencia de los sistemas de produccion de rumiantes se han establecido estrategias nutricionales que tienen como objetivo manipular la fermentacion ruminal mediante el uso de aditivos en la dieta, como monensina, sebo, tampones, compuestos de nitrogeno, probioticos, etc. Estos aditivos permiten cambiar el proceso de fermentacion y mejorar la eficiencia animal, ademas disminuyen la perdida de energia. El objetivo de este trabajo es revisar los procesos fermentativos que tienen lugar en el rumen y aplicar los fundamentos de estos en el desarrollo de nuevas estrategias nutricionales que pudieran ayudar a mejorar los procesos de digestion, de manera que se alcance una maxima produccion

Trabulsiella odontotermitis represents a novel species in the genus Trabulsiella with no complete genome reported yet. Here, we describe the draft genome sequences of five isolates from termites present in the north of Mexico, which have an interesting pool of genes related to cellulose degradation with biotechnological application.

The draft genome of Citrobacter sp. CtB7.12, isolated from termite gut, is presented here. This organism has been reported as a cellulolytic bacterium, which is biotechnologically important because it can be used as a gene donor for the ethanol and biofuel industries.

The aim of this study was to evaluate the influence of different concentrations of inulin and pectin as fat substitutes on the chemical composition, texture, and sensory acceptance of frankfurter sausages. Six treatments were evaluated to test fat replacement: control (T0); low fat control (T1); low fat with 15% inulin (T2); low fat with 30% inulin (T3); low fat with 7.5% inulin and 7.5% pectin (T4); and low fat with 15% inulin and 15% pectin (T5). The addition of fibers increased the yield (T3 and T5; 98.96%), and the color parameters were slightly reduced (T3). Moisture (61.14%) and ashes (6.96%) of sausages with inulin and pectin were higher (T5), while shear force, hardness, fracturability, gumminess, and chewiness (T3 and T5) were slightly lower than those of the control. The addition of inulin (T2) increased the sensory acceptance of the sausages (5.75). Fat can be replaced with inulin and pectin in frankfurter sausages to produce healthy and functional products.

Hispanics comprise the largest minority group in the United States (US); it is estimated that by 2050, they will represent about 25 % of the US population [1]. The US Office of Management and Budget defines the terms “Hispanic,” “Latino,” and “of Spanish origin” as a “person of Cuban, Mexican (ME), Puerto Rican (PR), Central American (CA) or South American (SA), or other Spanish culture origin, regardless of race” [2]. Sometimes the terms “Latino” and “Hispanic” are used interchangeably; however, these terms are not used in Hispanic American countries; people from these countries prefer to refer themselves with national terms like “Colombians,” “Costa Ricans,” “Mexicans,” or “Dominicans.” Although, most of the Hispanics share a language (Spanish) and some cultural values, they should not be considered a homogenous group; Hispanics came from different sociocultural contexts [3]. Studies have demonstrated that Hispanics have differences in several socioeconomic indicators, health outcomes, lifestyle behaviors, and cultural characteristics [4, 5]. This chapter focuses on one of the main differences found within the Hispanic population—food-related practices.