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1. Azithromycin disrupts apicoplast biogenesis in replicating and dormant liver stages of the relapsing malaria parasites Plasmodium vivax and Plasmodium cynomolgi

Author

Amanzougaghene, Nadia, Tajeri, Shahin, Franetich, Jean-François, Ashraf, Kutub, Soulard, Valérie, Bigeard, Pierre, Guindo, Cheick Oumar, Bouillier, Camille, Lemaitre, Julien, Relouzat, Francis, Legrand, Roger, Kocken, Clemens H.M., Zeeman, Anne-Marie, Roobsoong, Wanlapa, Sattabongkot, Jetsumon, Yang, Zhaoqing, Snounou, Georges, and Mazier, Dominique

Published
2024
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2. Novel thienopyrimidones targeting hepatic and erythrocytic stages of Plasmodium parasites with increased microsomal stability

Author

Lagardère, Prisca, Mustière, Romain, Amanzougaghene, Nadia, Hutter, Sébastien, Casanova, Marion, Franetich, Jean-François, Tajeri, Shahin, Malzert-Fréon, Aurélie, Corvaisier, Sophie, Since, Marc, Azas, Nadine, Vanelle, Patrice, Verhaeghe, Pierre, Primas, Nicolas, Mazier, Dominique, Masurier, Nicolas, and Lisowski, Vincent

Published
2023
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4. Synthesis of antiplasmodial 2-aminothieno[3,2-d]pyrimidin-4(3H)-one analogues using the scaffold hopping strategy

Author

Mustière, Romain, Lagardère, Prisca, Hutter, Sébastien, Dell’Orco, Viviana, Amanzougaghene, Nadia, Tajeri, Shahin, Franetich, Jean-François, Corvaisier, Sophie, Since, Marc, Malzert-Fréon, Aurélie, Masurier, Nicolas, Lisowski, Vincent, Verhaeghe, Pierre, Mazier, Dominique, Azas, Nadine, Vanelle, Patrice, and Primas, Nicolas

Published
2022
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10. Novel Thienopyrimidones Targeting Hepatic and Erythrocytic Stages of Plasmodium Parasites with Increased Microsomal Stability

Author

Lagardère, Prisca, primary, Mustière, Romain, additional, Amanzougaghene, Nadia, additional, Hutter, Sébastien, additional, Casanova, Marion, additional, Franetich, Jean-François, additional, Tajeri, Shahin, additional, Malzert-Fréon, Aurélie, additional, Corvaisier, Sophie, additional, Since, Marc, additional, Azas, Nadine, additional, Vanelle, Patrice, additional, Verhaeghe, Pierre, additional, Primas, Nicolas, additional, Mazier, Dominique, additional, Masurier, Nicolas, additional, and Lisowski, Vincent, additional

Published
2023
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12. Rapid and Specific Action of Methylene Blue against Plasmodium Transmission Stages

Author

Saison, Nathanaël, primary, Franetich, Jean-François, additional, Pinilla, Yudi T., additional, Hoffmann, Anton, additional, Boussougou-Sambe, Stravensky T., additional, Ngossanga, Barclaye, additional, Tefit, Maurel, additional, Ashraf, Kutub, additional, Amanzougaghene, Nadia, additional, Tajeri, Shahin, additional, Adegnika, Ayola A., additional, Mazier, Dominique, additional, and Borrmann, Steffen, additional

Published
2022
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13. 4-Substituted Thieno[3,2-d]pyrimidines as Dual-Stage Antiplasmodial Derivatives

Author

Lagardère, Prisca, primary, Mustière, Romain, additional, Amanzougaghene, Nadia, additional, Hutter, Sébastien, additional, Franetich, Jean-François, additional, Azas, Nadine, additional, Vanelle, Patrice, additional, Verhaeghe, Pierre, additional, Primas, Nicolas, additional, Mazier, Dominique, additional, Masurier, Nicolas, additional, and Lisowski, Vincent, additional

Published
2022
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14. Booklice Liposcelis bostrychophila Naturally Infected by Rickettsia felis Cause Fever and Experimental Pneumonia in Mammals

Author

Mediannikov, Oleg, primary, Bechah, Yassina, additional, Amanzougaghene, Nadia, additional, Lepidi, Hubert, additional, Bassene, Hubert, additional, Sambou, Masse, additional, Lienhard, Charles, additional, Benkacimi, Linda, additional, Dieme, Constentin, additional, Sokhna, Cheikh, additional, Fenollar, Florence, additional, and Raoult, Didier, additional

Published
2022
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15. Enteric pathogenic bacteria and resistance gene carriage in the homeless population in Marseille, France

Author

Ly, Tran Duc Anh, Hadjadj, Linda, Hoang, van Thuan, Goumbala, Ndiaw, Dao, Thi Loi, Badiaga, Sekene, Tissot-Dupont, Herve, Brouqui, Philippe, Raoult, Didier, Rolain, Jean-Marc, Gautret, Philippe, Bosson-Vanga, Henriette, Primas, Nicolas, Franetich, Jean-François, Lavazec, Catherine, Gomez, Lina, Ashraf, Kutub, Tefit, Maurel, Soulard, Valérie, Dereuddre-Bosquet, Nathalie, Le Grand, Roger, Donnette, Mélanie, Mustière, Romain, Amanzougaghene, Nadia, Tajeri, Shahin, Suzanne, Peggy, Malzert-Fréon, Aurélie, Rault, Sylvain, Vanelle, Patrice, Hutter, Sébastien, Cohen, Anita, Snounou, Georges, Roques, Pierre, Azas, Nadine, Lagardère, Prisca, Lisowski, Vincent, Masurier, Nicolas, Nguyen, Michel, Paloque, Lucie, Benoit-Vical, Françoise, Verhaeghe, Pierre, Mazier, Dominique, Vecteurs - Infections tropicales et méditerranéennes (VITROME), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées (IRBA), Microbes évolution phylogénie et infections (MEPHI), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU), Thai Binh University of Medicine and Pharmacy, Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées [Brétigny-sur-Orge] (IRBA), and Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)

Subjects
medicine.disease_cause, Microbiology, law.invention, Tropheryma whipplei, 03 medical and health sciences, 0302 clinical medicine, Antibiotic resistance, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, law, medicine, [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology, 030212 general & internal medicine, Escherichia coli, Gene, Polymerase chain reaction, [SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases, 0303 health sciences, General Immunology and Microbiology, biology, 030306 microbiology, 1. No poverty, Pathogenic bacteria, General Medicine, biology.organism_classification, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, 3. Good health, Carriage, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Antibiotic resistance genes
Abstract

We aimed to assess the prevalence of pathogenic bacteria and resistance genes in rectal samples collected among homeless persons in Marseille, France. In February 2014 we enrolled 114 sheltered homeless adults who completed questionnaires and had rectal samples collected. Eight types of enteric bacteria and 15 antibiotic resistance genes (ARGs) were sought by real-time polymerase chain reaction (qPCR) performed directly on rectal samples. ARG-positive samples were further tested by conventional PCR and sequencing. We evidenced a 17.5% prevalence of gastrointestinal symptoms, a 9.6% prevalence of enteric pathogenic bacteria carriage, including Escherichia coli pathotypes (8.7%) and Tropheryma whipplei (0.9%). Only 2 persons carried blaCTX-M-15 resistance genes (1.8%), while other genes, including carbapenemase-encoding genes and colistin-resistance genes, (mcr-1 to mcr-6, mcr-8) were not detected. Our results suggest that sheltered homeless persons in Marseille do not have a high risk of harbouring gastrointestinal antibiotic resistant bacteria.

Published
2021

18. New Antiplasmodial 4-Amino-Thieno[3,2-D]Pyrimidines with Improved Intestinal Permeability and Microsomal Stability

Author

Lagardère, Prisca, primary, Mustière, Romain, additional, Amanzougaghene, Nadia, additional, Hutter, Sébastien, additional, Casanova, Marion, additional, Franetich, Jean-François, additional, Tajeri, Shahin, additional, Malzert-Fréon, Aurélie, additional, Corvaisier, Sophie, additional, Azas, Nadine, additional, Vanelle, Patrice, additional, Verhaeghe, Pierre, additional, Primas, Nicolas, additional, Mazier, Dominique, additional, Masurier, Nicolas, additional, and Lisowski, Vincent, additional

Published
2022
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19. Synthesis of Antiplasmodial 2-Aminothieno[3,2-D]Pyrimidin-4(3h)-One Analogues Using the Scaffold Hopping Strategy

Author

Mustière, Romain, primary, Lagardère, Prisca, additional, Hutter, Sébastien, additional, Dell'Orco, Viviana, additional, Amanzougaghene, Nadia, additional, Tajeri, Shahin, additional, Franetich, Jean-François, additional, Corvaisier, Sophie, additional, Since, Marc, additional, Malzert-Fréon, Aurélie, additional, Masurier, Nicolas, additional, Lisowski, Vincent, additional, Verhaeghe, Pierre, additional, Mazier, Dominique, additional, Azas, Nadine, additional, Vanelle, Patrice, additional, and Primas, Nicolas, additional

Published
2022
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20. Artemisinin-independent inhibitory activity of Artemisia sp. infusions against different Plasmodium stages including relapse-causing hypnozoites

Author

Ashraf, Kutub, primary, Tajeri, Shahin, additional, Arnold, Christophe-Sébastien, additional, Amanzougaghene, Nadia, additional, Franetich, Jean-François, additional, Vantaux, Amélie, additional, Soulard, Valérie, additional, Bordessoulles, Mallaury, additional, Cazals, Guillaume, additional, Bousema, Teun, additional, van Gemert, Geert-Jan, additional, Le Grand, Roger, additional, Dereuddre-Bosquet, Nathalie, additional, Barale, Jean-Christophe, additional, Witkowski, Benoit, additional, Snounou, Georges, additional, Duval, Romain, additional, Botté, Cyrille Y, additional, and Mazier, Dominique, additional

Published
2021
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21. A New Thienopyrimidinone Chemotype Shows Multistage Activity against Plasmodium falciparum, Including Artemisinin-Resistant Parasites

Author

Bosson-Vanga, Henriette, primary, Primas, Nicolas, additional, Franetich, Jean-François, additional, Lavazec, Catherine, additional, Gomez, Lina, additional, Ashraf, Kutub, additional, Tefit, Maurel, additional, Soulard, Valérie, additional, Dereuddre-Bosquet, Nathalie, additional, Le Grand, Roger, additional, Donnette, Mélanie, additional, Mustière, Romain, additional, Amanzougaghene, Nadia, additional, Tajeri, Shahin, additional, Suzanne, Peggy, additional, Malzert-Fréon, Aurélie, additional, Rault, Sylvain, additional, Vanelle, Patrice, additional, Hutter, Sébastien, additional, Cohen, Anita, additional, Snounou, Georges, additional, Roques, Pierre, additional, Azas, Nadine, additional, Lagardère, Prisca, additional, Lisowski, Vincent, additional, Masurier, Nicolas, additional, Nguyen, Michel, additional, Paloque, Lucie, additional, Benoit-Vical, Françoise, additional, Verhaeghe, Pierre, additional, and Mazier, Dominique, additional

Published
2021
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22. Multiple vector-borne pathogens of domestic animals in Egypt

Author

Abdullah, Hend, Amanzougaghene, Nadia, Dahmana, Handi, Louni, Meriem, Raoult, Didier, Mediannikov, Oleg, National Research Centre [Giza, Egypt], Microbes évolution phylogénie et infections (MEPHI), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Vecteurs - Infections tropicales et méditerranéennes (VITROME), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées [Brétigny-sur-Orge] (IRBA), Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille), National Research Centre, Dokki, Giza, 12622, Egypt, and Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées (IRBA)

Subjects
RC955-962, Rickettsiales, Pathology and Laboratory Medicine, Biochemistry, Geographical Locations, Camels, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Arctic medicine. Tropical medicine, Prevalence, Medicine and Health Sciences, Protozoan Infections, Animal, Mammals, [SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases, Eukaryota, Bacterial Infections, Ruminants, Filariasis, Bacterial Pathogens, Nucleic acids, Ribosomal RNA, Medical Microbiology, Animals, Domestic, Vertebrates, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Egypt, Pathogens, Public aspects of medicine, RA1-1270, Research Article, Cell biology, Asia, Anaplasma, Cellular structures and organelles, Babesia, Vector Borne Diseases, Microbiology, Dogs, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, Bovines, Animals, [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology, Non-coding RNA, Microbial Pathogens, Filarioidea, Animal Pathogens, Sheep, Bacteria, Organisms, Biology and Life Sciences, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Cross-Sectional Studies, Amniotes, People and Places, Africa, Phyllachorales, RNA, Cattle, Zoology, Ribosomes
Abstract

Vector Borne Diseases (VBDs) are considered emerging and re-emerging diseases that represent a global burden. The aim of this study was to explore and characterize vector-borne pathogens in different domestic animal hosts in Egypt. A total of 557 blood samples were collected from different animals using a convenience sampling strategy (203 dogs, 149 camels, 88 cattle, 26 buffaloes, 58 sheep and 33 goats). All samples were tested for multiple pathogens using quantitative PCR and standard PCR coupled with sequencing. We identified Theileria annulata and Babesia bigemina in cattle (15.9 and 1.1%, respectively), T. ovis in sheep and buffaloes (8.6 and 7.7%, respectively) and Ba. canis in dogs (0.5%) as well as Anaplasma marginale in cattle, sheep and camels (20.4, 3.4 and 0.7%, respectively) and Coxiella burnetii in sheep and goats (1.7 and 3%; respectively). New genotypes of An. centrale, An. ovis, An. platys-like and Borrelia theileri were found in cattle (1.1,3.4, 3.4 and 3.4%, respectively), An. platys-like in buffaloes (7.7%), An. marginale, An. ovis, An. platys-like and Bo. theileri in sheep (3.4, 1.7, 1.7 and 3.4%, respectively), An. platys, An. platys-like and Setaria digitata in camels (0.7, 5.4 and 0.7%, respectively) and Rickettsia africae-like, An. platys, Dirofilaria repens and Acanthocheilonema reconditum in dogs (1.5, 3.4, 1 and 0.5%, respectively). Co-infections were found in cattle, sheep and dogs (5.7, 1.7, 0.5%, respectively). For the first time, we have demonstrated the presence of several vector-borne zoonoses in the blood of domestic animals in Egypt. Dogs and ruminants seem to play a significant role in the epidemiological cycle of VBDs., Author summary Vector Borne Diseases (VBDs) are considered emerging and re-emerging diseases that represent a global burden. Diagnosis of these diseases is challenging due to nonspecific febrile illness, difficulty of isolation, and cross-reactivity of serological methods. Therefore, the current study is the first large-scale epidemiological study in which molecular screening and characterization of multiple vector-borne pathogens in different animal hosts were performed to better understand the endemicity of VBDs in Egypt. We detected for the first time Anaplasma centrale, An. ovis, a novel An. platys-like and Borrelia theileri in cattle, a new An. platys-like in buffaloes, An. marginale, An. ovis, a new An. platys-like and Bo. theileri in sheep, An. platys, a new An. platys-like and Setaria digitata in camels and Rickettsia africae-like, An. platys, Dirofilaria repens and Acanthocheilonema reconditum in dogs, in Egypt. These results imply that ruminants and dogs in Egypt are reservoirs for several neglected, emerging and re-emerging potentially new vector-borne pathogens that have significant implications in human health.

Published
2021

23. Assessment of the burden of malaria and bacteraemia by retrospective molecular diagnosis in febrile illnesses and first-line anti-infectives in Côte d'Ivoire

Author

Ehounoud, Bilé Cyrille Hervé, Boumbanda Koyo, Celia Scherelle, Doua Bongue, Landry, Cortaredona, Sébastien, N’Douba Kakou, Adèle, Konan, Djanwai Berenger, Kouassi Patrick, Yao, Amanzougaghene, Nadia, N’Guessan, Jean-David, Davoust, Bernard, Raoult, Didier, Mediannikov, Oleg, Fenollar, Florence, Vecteurs - Infections tropicales et méditerranéennes (VITROME), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées [Brétigny-sur-Orge] (IRBA), Centre Médico Social Wale, Yamoussoukro, Université Félix Houphouët-Boigny (UFHB), Microbes évolution phylogénie et infections (MEPHI), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille), and Institut de Recherche Biomédicale des Armées (IRBA)-Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)

Subjects
[SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2021

24. The Host Protein Aquaporin-9 is Required for Efficient Plasmodium falciparum Sporozoite Entry into Human Hepatocytes

Author

Amanzougaghene, Nadia, Tajeri, Shahin, Yalaoui, Samir, Lorthiois, Audrey, Soulard, Valérie, Gego, Audrey, Rametti, Armelle, Risco-Castillo, Veronica, Moreno, Alicia, Tefit, Maurel, van Gemert, Geert-Jan, Sauerwein, Robert, Vaillant, Jean-Christophe, Ravassard, Philippe, Pérignon, Jean-Louis, Froissard, Patrick, Mazier, Dominique, Franetich, Jean-François, Centre d'Immunologie et des Maladies Infectieuses (CIMI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Immunobiologie Cellulaire et Moléculaire des Infections Parasitaires, Université Pierre et Marie Curie - Paris 6 (UPMC)-IFR113-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Cochin (IC UM3 (UMR 8104 / U1016)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Immunité et Infection, Laboratoire de Biochimie Pharmaceutique, Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Dynamic Microbiology - EA 7380 (DYNAMIC), École nationale vétérinaire - Alfort (ENVA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est (UPE)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Service de Parasitologie - Mycologie [CHU Saint-Antoine], CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Centre d'Immunologie et de Maladies Infectieuses (CIMI), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Service de Chirurgie Digestive, Hépato-Bilio-pancréatique et Transplantation Hépatique [CHU Pitié-Salpétrière], CHU Pitié-Salpêtrière [AP-HP], Institut du Cerveau = Paris Brain Institute (ICM), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR113-Université Pierre et Marie Curie - Paris 6 (UPMC), École nationale vétérinaire d'Alfort (ENVA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU), Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut du Cerveau et de la Moëlle Epinière = Brain and Spine Institute (ICM), Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes (UR), and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)

Subjects
sporozoites, CD81, liver stage, parasitic diseases, Plasmodium falciparum, Aquaporin-9, [SDV.IMM]Life Sciences [q-bio]/Immunology, hepatocytes
Abstract

International audience; Hepatocyte invasion by Plasmodium sporozoites represents a promising target for innovative antimalarial therapy, but the molecular events mediating this process are still largely uncharacterized. We previously showed that Plasmodium falciparum sporozoite entry into hepatocytes strictly requires CD81. However, CD81-overexpressing human hepatoma cells remain refractory to P. falciparum infection, suggesting the existence of additional host factors necessary for sporozoite entry. Here, through differential transcriptomic analysis of human hepatocytes and hepatoma HepG2-CD81 cells, the transmembrane protein Aquaporin-9 (AQP9) was found to be among the most downregulated genes in hepatoma cells. RNA silencing showed that sporozoite invasion of hepatocytes requires AQP9 expression. AQP9 overexpression in hepatocytes increased their permissiveness to P. falciparum. Moreover, chemical disruption with the AQP9 inhibitor phloretin markedly inhibited hepatocyte infection. Our findings identify AQP9 as a novel host factor required for P. falciparum sporozoite hepatocyte-entry and indicate that AQP9 could be a potential therapeutic target.

Published
2021

25. Artemisinin-independent inhibitory activity ofArtemisiasp. infusions against differentPlasmodiumstages including relapse-causing hypnozoites

Author

Ashraf, Kutub, primary, Tajeri, Shahin, additional, Arnold, Christophe-Sébastien, additional, Amanzougaghene, Nadia, additional, Franetich, Jean-François, additional, Vantaux, Amélie, additional, Soulard, Valérie, additional, Bordessoulles, Mallaury, additional, Cazals, Guillaume, additional, Bousema, Teun, additional, van Gemert, Geert-Jan, additional, Grand, Roger Le, additional, Dereuddre-Bosquet, Nathalie, additional, Barale, Jean-Christophe, additional, Witkowski, Benoit, additional, Snounou, Georges, additional, Duval, Romain, additional, Botté, Cyrille Y., additional, and Mazier, Dominique, additional

Published
2021
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26. 2-Phenoxy-3-Trichloromethylquinoxalines Are Antiplasmodial Derivatives with Activity against the Apicoplast of Plasmodium falciparum

Author

Amrane, Dyhia, primary, Arnold, Christophe-Sébastien, additional, Hutter, Sébastien, additional, Sanz-Serrano, Julen, additional, Collia, Miguel, additional, Azqueta, Amaya, additional, Paloque, Lucie, additional, Cohen, Anita, additional, Amanzougaghene, Nadia, additional, Tajeri, Shahin, additional, Franetich, Jean-François, additional, Mazier, Dominique, additional, Benoit-Vical, Françoise, additional, Verhaeghe, Pierre, additional, Azas, Nadine, additional, Vanelle, Patrice, additional, Botté, Cyrille, additional, and Primas, Nicolas, additional

Published
2021
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27. 4-Substituted Thieno[3,2- d ]pyrimidines as Dual-Stage Antiplasmodial Derivatives.

Author

Lagardère, Prisca, Mustière, Romain, Amanzougaghene, Nadia, Hutter, Sébastien, Franetich, Jean-François, Azas, Nadine, Vanelle, Patrice, Verhaeghe, Pierre, Primas, Nicolas, Mazier, Dominique, Masurier, Nicolas, and Lisowski, Vincent

Subjects
MALARIA, PLASMODIUM, PYRIMIDINES
Abstract

Malaria remains one of the major health problems worldwide. The increasing resistance of Plasmodium to approved antimalarial drugs requires the development of novel antiplasmodial agents that can effectively prevent and/or treat this disease. Based on the structure of Gamhepathiopine, a 2-tert-butylaminothieno[3,2-d]pyrimidin-4(3H)-one hit, active on the sexual and asexual stages of the parasite and thanked for the introduction of various substituents at position 4 of the thienopyrimidine core by nucleophilic aromatic substitution and pallado-catalyzed coupling reactions, a series of 4-substituted thieno[3,2-d]pyrimidines were identified as displaying in vitro activities against both the erythrocytic stage of P. falciparum and the hepatic stage of P. berghei. Among the 28 compounds evaluated, the chloro analogue of Gamhepathiopine showed good activity against the erythrocytic stage of P. falciparum, moderate toxicity on HepG2, and better activity against hepatic P. berghei parasites, compared to Gamhepathiopine. [ABSTRACT FROM AUTHOR]

Published
2022
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28. Where Are We With Human Lice? A Review of the Current State of Knowledge

Author

Amanzougaghene, Nadia, Fenollar, Florence, Raoult, Didier, Mediannikov, Oleg, Vecteurs - Infections tropicales et méditerranéennes (VITROME), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées [Brétigny-sur-Orge] (IRBA), Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille), Microbes évolution phylogénie et infections (MEPHI), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), ANR-10-IAHU-0003,Méditerranée Infection,I.H.U. Méditerranée Infection(2010), and Institut de Recherche Biomédicale des Armées (IRBA)-Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)

Subjects
Insecticides, disease-vector, Review, phylogeny, Evolution, Molecular, Insecticide Resistance, Cellular and Infection Microbiology, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Animals, Humans, [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology, [SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases, Genome, Bacteria, biology, Pediculus humanus, Pediculus, Lice Infestations, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Insect Vectors, Mitochondria, Phylogeography, Communicable Disease Control, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, epidemiology, control
Abstract

International audience; Pediculus humanus is an obligate bloodsucking ectoparasite of human that includes two ecotypes, head louse and body louse, which differ slightly in morphology and biology, but have distinct ecologies. Phylogenetically, they are classified on six mitochondrial clades (A, B, C, D, E, and F), head louse encompasses the full genetic diversity of clades, while body louse belongs to clades A and D. Recent studies suggested that not only body louse, but also head louse can transmit disease, which warrants greater attention as a serious public health problem. The recent sequencing of body louse genome confirmed that P. humanus has the smallest genome of any hemimetabolous insect reported to date, and also revealed numerous interesting characteristics in the nuclear and mitochondrial genomes. The transcriptome analyses showed that body and head lice were almost genetically identical. Indeed, the phenotypic flexibility associated with the emergence of body lice, is probably a result of regulatory changes, perhaps epigenetic in origin, triggered by environmental signals. Current lice control strategies have proven unsuccessful. For instance, ivermectin represents a relatively new and very promising pediculicide. However, ivermectin resistance in the field has begun to be reported. Therefore, novel opportunities for pest control strategies are needed. Our objective here is to review the current state of knowledge on the biology, epidemiology, phylogeny, disease-vector and control of this fascinating and very intimate human parasite.

Published
2020

29. Additional file 2 of Molecular identification of head lice collected in Franceville (Gabon) and their associated bacteria

Author

Boumbanda-Koyo, Celia Scherelle, Mediannikov, Oleg, Amanzougaghene, Nadia, Oyegue-Liabagui, Sandrine Lydie, Imboumi-Limoukou, Roméo Karl, Raoult, Didier, Lekana-Douki, Jean Bernard, and Fenollar, Florence

Subjects
mental disorders, parasitic diseases, biochemical phenomena, metabolism, and nutrition, bacterial infections and mycoses, skin and connective tissue diseases
Abstract

Additional file 2: Table S2. Number of lice carried by patients with lice positive for Acinetobacter baumannii and a breakdown by patient.

Published
2020
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30. [A health service]

Author

Mediannikov, Oleg, Fenollar, Florence, Davoust, Bernard, Amanzougaghene, Nadia, Lepidi, Hubert, Arzouni, Jean-Pierre, Diatta, Georges, Sokhna, Cheikh, Delerce, Jeremy, Levasseur, Anthony, Raoult, Didier, Microbes évolution phylogénie et infections (MEPHI), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Vecteurs - Infections tropicales et méditerranéennes (VITROME), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées (IRBA), and Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées [Brétigny-sur-Orge] (IRBA)

Subjects
MESH: Community Health Services, [SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases, Health Priorities, MESH: Hospitals, General, MESH: State Medicine, Models, Theoretical, Hospitals, General, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, State Medicine, MESH: Health Priorities, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Preventive Health Services, MESH: Preventive Health Services, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology, Community Health Services, MESH: Models, Theoretical, Mexico, MESH: Mexico, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2020

32. Chloroquine Potentiates Primaquine Activity against Active and Latent Hepatic Plasmodia Ex Vivo : Potentials and Pitfalls

Author

Dembélé, Laurent, primary, Franetich, Jean-François, additional, Soulard, Valérie, additional, Amanzougaghene, Nadia, additional, Tajeri, Shahin, additional, Bousema, Teun, additional, van Gemert, Geert-Jan, additional, Le Grand, Roger, additional, Dereuddre-Bosquet, Nathalie, additional, Baird, J. Kevin, additional, Mazier, Dominique, additional, and Snounou, Georges, additional

Published
2020
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35. Artemisinin-independent inhibitory activity of Artemisia sp. infusions against different Plasmodium stages including relapse-causing hypnozoites.

Author

Ashraf, Kutub, Tajeri, Shahin, Arnold, Christophe-Sébastien, Amanzougaghene, Nadia, Franetich, Jean-François, Vantaux, Amélie, Soulard, Valérie, Bordessoulles, Mallaury, Cazals, Guillaume, Bousema, Teun, van Gemert, Geert-Jan, Le Grand, Roger, Dereuddre-Bosquet, Nathalie, Barale, Jean-Christophe, Witkowski, Benoit, Snounou, Georges, Duval, Romain, Botté, Cyrille Y., and Mazier, Dominique

Published
2022
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37. Complexin in ivermectin resistance in body lice

Author

Amanzougaghene, Nadia, Fenollar, Florence, Nappez, Claude, Ben-Amara, Amira, Decloquement, Philippe, Azza, Said, Bechah, Yassina, Chabriere, Eric, Raoult, Didier, Mediannikov, Oleg, Microbes évolution phylogénie et infections (MEPHI), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Vecteurs - Infections tropicales et méditerranéennes (VITROME), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées [Brétigny-sur-Orge] (IRBA), Institut de Recherche pour le Développement (IRD), ANR-10-IAHU-0003,Méditerranée Infection,I.H.U. Méditerranée Infection(2010), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU), and Institut de Recherche Biomédicale des Armées (IRBA)-Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)

Subjects
Proteomics, Insecticides, lcsh:QH426-470, Nerve Tissue Proteins, Body lice, Insecticide Resistance, RNA interference, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], parasitic diseases, Binding analysis, Animals, [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology, Amino Acid Sequence, RNA, Messenger, Cloning, Molecular, Ivermectin, Pediculus, Cell binding, Sequence Analysis, DNA, Lice Infestations, lcsh:Genetics, Transmembrane transport proteins, Gene Expression Regulation, Insect Proteins, Protein expression, Gene expression
Abstract

International audience; Ivermectin has emerged as very promising pediculicide, particularly in cases of resistance to commonly used pediculicides. Recently, however, the first field-evolved ivermectin-resistance in lice was reported. To gain insight into the mechanisms underlying ivermectin-resistance, we both looked for mutations in the ivermectin-target site (GluCl) and searched the entire proteome for potential new loci involved in resistance from laboratory susceptible and ivermectin-selected resistant body lice. Polymorphism analysis of cDNA GluCl showed no non-silent mutations. Proteomic analysis identified 22 differentially regulated proteins, of which 13 were upregulated and 9 were downregulated in the resistant strain. We evaluated the correlation between mRNA and protein levels by qRT-PCR and found that the trend in transcriptional variation was consistent with the proteomic changes. Among differentially expressed proteins, a complexin i.e. a neuronal protein which plays a key role in regulating neurotransmitter release, was shown to be the most significantly down-expressed in the ivermectin-resistant lice. Moreover, DNA-mutation analysis revealed that some complexin transcripts from resistant lice gained a premature stop codon, suggesting that this down-expression might be due, in part, to secondary effects of a nonsense mutation inside the gene. We further confirmed the association between complexin and ivermectin-resistance by RNA-interfering and found that knocking down the complexin expression induces resistance to ivermectin in susceptible lice. Our results provide evidence that complexin plays a significant role in regulating ivermectin resistance in body lice and represents the first evidence that links complexin to insecticide resistance.

Published
2018

40. Detection of bacterial pathogens including potential new species in human head lice from Mali

Author

Amanzougaghene, Nadia, Fenollar, Florence, Sangaré, Abdoul Karim, Sissoko, Mahamadou S., Doumbo, Ogobara K., Raoult, Didier, Mediannikov, Oleg, Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR48, Institut des sciences biologiques (INSB-CNRS)-Institut des sciences biologiques (INSB-CNRS)-Centre National de la Recherche Scientifique (CNRS), Malaria Research and Training Center [Bamako, Mali], Université de Bamako, Sciences Economiques et Sociales de la Santé & Traitement de l'Information Médicale (SESSTIM - U912 INSERM - Aix Marseille Univ - IRD), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Malaria Research and Training Center (MRTC), Faculté de Médecine de Bamako, ANR-10-IAHU-0003,Méditerranée Infection,I.H.U. Méditerranée Infection(2010), INSB-INSB-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche pour le Développement (IRD)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Aix Marseille Université (AMU), COMBE, Isabelle, and Instituts Hospitalo-Universitaires - I.H.U. Méditerranée Infection - - Méditerranée Infection2010 - ANR-10-IAHU-0003 - IAHU - VALID

Subjects
lcsh:Medicine, Rickettsiales, Disease Vectors, Pathology and Laboratory Medicine, Mali, Polymerase Chain Reaction, Body lice, Database and Informatics Methods, Bartonella quintana, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Medicine and Health Sciences, lcsh:Science, skin and connective tissue diseases, Phylogeny, Pediculus, Eukaryota, Lice Infestations, Trench Fever, Insects, Infectious Diseases, Medical Microbiology, Coxiella burnetii, Head lice, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Sequence databases, Pathogens, Sequence Analysis, [SDV.MP.PAR] Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology, Lice, Research Article, DNA, Bacterial, Anaplasma, Arthropoda, Bioinformatics, Ehrlichia, Research and Analysis Methods, Microbiology, parasitic diseases, Animals, Humans, [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology, Microbial Pathogens, Bacteria, lcsh:R, Organisms, Biology and Life Sciences, bacterial infections and mycoses, Invertebrates, Insect Vectors, Species Interactions, Biological Databases, Scalp Dermatoses, bacteria, lcsh:Q, Bacterial pathogens
Abstract

International audience; In poor African countries, where no medical and biological facilities are available, the identification of potential emerging pathogens of concern at an early stage is challenging. Head lice, Pediculus humanus capitis, have a short life, feed only on human blood and do not transmit pathogens to their progeny. They are, therefore, a perfect tool for the xenodiagnosis of current or recent human infection. This study assessed the occurrence of bacterial pathogens from head lice collected in two rural villages from Mali, where a high frequency of head lice infestation had previously been reported, using molecular methods. Results show that all 600 head lice, collected from 117 individuals, belonged to clade E, specific to West Africa. Bartonella quintana, the causative agent of trench fever, was identified in three of the 600 (0.5%) head lice studied. Our study also shows, for the first time, the presence of the DNA of two pathogenic bacteria, namely Coxiella burnetii (5.1%) and Rickettsia aeschlimannii (0.6%), detected in human head lice, as well as the DNA of potential new species from the Anaplasma and Ehrlichia genera of unknown pathogenicity. The finding of several Malian head lice infected with B. quintana, C. burnetii, R. aeschlimannii, Anaplasma and Ehrlichia is alarming and highlights the need for active survey programs to define the public health consequences of the detection of these emerging bacterial pathogens in human head lice.

Published
2017

41. Head Lice of Pygmies Reveal the Presence of Relapsing Fever Borreliae in the Republic of Congo

Author

Amanzougaghene, Nadia, Akiana, Jean, Ndombe, Géor Mongo, Davoust, Bernard, Nsana, Nardiouf Sjelin, Parra, Henri-Joseph, Fenollar, Florence, Raoult, Didier, Mediannikov, Oleg, Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR48, Institut des sciences biologiques (INSB-CNRS)-Institut des sciences biologiques (INSB-CNRS)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier et Universitaire de Brazzaville (CHUB), Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille), INSB-INSB-Centre National de la Recherche Scientifique (CNRS), and COMBE, Isabelle

Subjects
Male, lcsh:Arctic medicine. Tropical medicine, lcsh:RC955-962, Body lice, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, parasitic diseases, Animals, Humans, [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology, Borrelia infection, skin and connective tissue diseases, Phylogeny, [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, [SDV.MHEP.ME] Life Sciences [q-bio]/Human health and pathology/Emerging diseases, [SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases, Acinetobacter, Borrelia, lcsh:Public aspects of medicine, Pediculus, Relapsing Fever, Acinetobacter infections, lcsh:RA1-1270, Lice Infestations, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, [SDV.MHEP.CSC] Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, Congo, Haplotypes, Head lice, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Haplogroups, Female, [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, [SDV.MP.PAR] Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology
Abstract

International audience; BACKGROUND: Head lice, Pediculus humanus capitis, occur in four divergent mitochondrial clades (A, B, C and D), each having particular geographical distributions. Recent studies suggest that head lice, as is the case of body lice, can act as a vector for louse-borne diseases. Therefore, understanding the genetic diversity of lice worldwide is of critical importance to our understanding of the risk of louse-borne diseases.METHODOLOGY/PRINCIPAL FINDINGS: Here, we report the results of the first molecular screening of pygmies' head lice in the Republic of Congo for seven pathogens and an analysis of lice mitochondrial clades. We developed two duplex clade-specific real-time PCRs and identified three major mitochondrial clades: A,C, and D indicating high diversity among the head lice studied. We identified the presence of a dangerous human pathogen, Borrelia recurrentis, the causative agentof relapsing fever, in ten clade A head lice, which was not reported in the Republic of Congo, and B. theileri in one head louse. The results also show widespread infection among head lice with several species of Acinetobacter. A. junii was the most prevalent, followed by A. ursingii, A. baumannii, A. johnsonii, A. schindleri, A. lwoffii, A. nosocomialis and A. towneri. CONCLUSIONS/SIGNIFICANCE: Our study is the first to show the presence of B. recurrentis in African pygmies' head lice in the Republic of Congo. This study isalso the first to report the presence of DNAs of B. theileri and several species of Acinetobacter in human head lice. Further studies are needed to determinewhether the head lice can transmit these pathogenic bacteria from person to another.

Published
2016

42. High Ancient Genetic Diversity of Human Lice, Pediculus humanus, from Israel Reveals New Insights into the Origin of Clade B Lice

Author

Amanzougaghene, Nadia, Mumcuoglu, Kosta Y., Fenollar, Florence, Alfi, Shir, Yesilyurt, Gonca, Raoult, Didier, Mediannikov, Oleg, Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR48, INSB-INSB-Centre National de la Recherche Scientifique (CNRS), Kuvin Center for the Study of Infectious and Tropical Diseases, The Hebrew University of Jerusalem (HUJ), and Institut des sciences biologiques (INSB-CNRS)-Institut des sciences biologiques (INSB-CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Epidemiology, lcsh:Medicine, Artificial Gene Amplification and Extension, Disease Vectors, Biochemistry, Body lice, Abdomen, Medicine and Health Sciences, Israel, lcsh:Science, History, Ancient, Phylogeny, Likelihood Functions, Ancient DNA, [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE], Pediculus, Cytochromes b, humanities, Polymerase chain reaction, Insects, Nucleic acids, Head lice, Anatomy, Lice, Research Article, Arthropoda, Molecular Sequence Data, Research and Analysis Methods, Real-Time Polymerase Chain Reaction, parasitic diseases, Genetics, Animals, Humans, [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology, Molecular Biology Techniques, Molecular Biology, Ovum, Evolutionary Biology, Population Biology, Base Sequence, lcsh:R, Organisms, Biology and Life Sciences, Paleontology, Genetic Variation, DNA, Invertebrates, Insect Vectors, Earth sciences, Haplotypes, RNA, Ribosomal, Haplogroups, lcsh:Q, Paleogenetics, Sequence Alignment, Population Genetics
Abstract

International audience; The human head louse, Pediculus humanus capitis, is subdivided into several significantly divergent mitochondrial haplogroups, each with particular geographical distributions. Historically, they are among the oldest human parasites, representing an excellent marker for tracking older events in human evolutionary history. In this study, ancient DNA analysis using real-time polymerase chain reaction (qPCR), combined with conventional PCR, was applied to the remains of twenty-four ancient head lice and their eggs from the Roman period which were recovered from Israel. The lice and eggs were found in three combs, one of which was recovered from archaeological excavations in the Hatzeva area of the Judean desert, and two of which found in Moa, in the Arava region, close to the Dead Sea. Results show that the head lice remains dating approximately to 2,000 years old have a cytb haplogroup A, which is worldwide in distribution, and haplogroup B, which has thus far only been found in contemporary lice from America, Europe, Australia and, most recently, Africa. More specifically, this haplogroup B has a B36 haplotype, the most common among B haplogroups, and has been present in America for at least 4,000 years. The present findings confirm that clade B lice existed, at least in the Middle East, prior to contacts between Native Americans and Europeans. These results support a Middle Eastern origin for clade B followed by its introduction into the New World with the early peoples. Lastly, the presence of Acinetobacter baumannii DNA was demonstrated by qPCR and sequencing in four head lice remains belonging to clade A.

Published
2016

46. Molecular Survey of Head and Body Lice, <italic>Pediculus humanus</italic>, in France.

Author

Candy, Kerdalidec, Amanzougaghene, Nadia, Izri, Arezki, Brun, Sophie, Durand, Rémy, Louni, Meriem, Raoult, Didier, Fenollar, Florence, and Mediannikov, Oleg

Subjects
*PEDICULUS humanus, *ACINETOBACTER calcoaceticus, *DNA analysis
Abstract

Human lice, Pediculus humanus, are obligate blood-sucking parasites. Phylogenetically, they belong to several mitochondrial clades exhibiting some geographic differences. Currently, the body louse is the only recognized disease vector, with the head louse being proposed as an additional vector. In this article, we study the genetic diversity of head and body lice collected from Bobigny, a town located close to Paris (France), and look for louse-borne pathogens. By amplifying and sequencing the cytb gene, we confirmed the presence of clades A and B in France. Besides, by amplifying and sequencing both cytb and cox1 gene, we reported, for the first time, the presence of clade E, which has thus far only been found in lice from West Africa. DNA from Bartonella quintana was detected in 16.7% of body lice from homeless individuals, but in none of the head lice collected from 47 families. Acinetobacter DNA was detected in 11.5% of head lice belonging to all three clades and 29.1% of body lice. Six species of Acinetobacter were identified, including two potential new ones. Acinetobacter baumannii was the most prevalent, followed by Candidatus Acinetobacter Bobigny-1, Acinetobacter calcoaceticus, Acinetobacter nosocomialis, Acinetobacter junii, and Candidatus Acinetobacter Bobigny-2. Body lice were found to be infected only with A. baumannii. These findings show for the first time, the presence of clade E head lice in France. This study is also the first to report the presence of DNAs of several species of Acinetobacter in human head lice in France. [ABSTRACT FROM AUTHOR]

Published
2018
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47. Multiple vector-borne pathogens of domestic animals in Egypt.

Author

Abdullah HHAM, Amanzougaghene N, Dahmana H, Louni M, Raoult D, and Mediannikov O

Subjects
Animals, Babesia genetics, Bacteria genetics, Bacterial Infections blood, Bacterial Infections epidemiology, Bacterial Infections veterinary, Cross-Sectional Studies, Egypt epidemiology, Filariasis epidemiology, Filariasis parasitology, Filariasis veterinary, Phyllachorales, Prevalence, Protozoan Infections, Animal blood, Protozoan Infections, Animal epidemiology, Protozoan Infections, Animal parasitology, Vector Borne Diseases blood, Vector Borne Diseases epidemiology, Animals, Domestic, Babesia isolation & purification, Bacteria isolation & purification, Filarioidea isolation & purification, Vector Borne Diseases veterinary
Abstract

Vector Borne Diseases (VBDs) are considered emerging and re-emerging diseases that represent a global burden. The aim of this study was to explore and characterize vector-borne pathogens in different domestic animal hosts in Egypt. A total of 557 blood samples were collected from different animals using a convenience sampling strategy (203 dogs, 149 camels, 88 cattle, 26 buffaloes, 58 sheep and 33 goats). All samples were tested for multiple pathogens using quantitative PCR and standard PCR coupled with sequencing. We identified Theileria annulata and Babesia bigemina in cattle (15.9 and 1.1%, respectively), T. ovis in sheep and buffaloes (8.6 and 7.7%, respectively) and Ba. canis in dogs (0.5%) as well as Anaplasma marginale in cattle, sheep and camels (20.4, 3.4 and 0.7%, respectively) and Coxiella burnetii in sheep and goats (1.7 and 3%; respectively). New genotypes of An. centrale, An. ovis, An. platys-like and Borrelia theileri were found in cattle (1.1,3.4, 3.4 and 3.4%, respectively), An. platys-like in buffaloes (7.7%), An. marginale, An. ovis, An. platys-like and Bo. theileri in sheep (3.4, 1.7, 1.7 and 3.4%, respectively), An. platys, An. platys-like and Setaria digitata in camels (0.7, 5.4 and 0.7%, respectively) and Rickettsia africae-like, An. platys, Dirofilaria repens and Acanthocheilonema reconditum in dogs (1.5, 3.4, 1 and 0.5%, respectively). Co-infections were found in cattle, sheep and dogs (5.7, 1.7, 0.5%, respectively). For the first time, we have demonstrated the presence of several vector-borne zoonoses in the blood of domestic animals in Egypt. Dogs and ruminants seem to play a significant role in the epidemiological cycle of VBDs., Competing Interests: The authors have declared that no competing interests exist.

Published
2021
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48. Assessment of the burden of malaria and bacteraemia by retrospective molecular diagnosis in febrile illnesses and first-line anti-infectives in Côte d'Ivoire.

Author

Ehounoud BCH, Boumbanda Koyo CS, Doua Bongue L, Cortaredona S, N'Douba Kakou A, Konan DB, Kouassi Patrick Y, Amanzougaghene N, N'Guessan JD, Davoust B, Raoult D, Mediannikov O, and Fenollar F

Subjects
Cote d'Ivoire epidemiology, Humans, Retrospective Studies, Bacteremia diagnosis, Bacteremia epidemiology, Malaria complications, Malaria epidemiology, Malaria, Falciparum
Abstract

Background: The aetiologies of fever are poorly understood in sub-Saharan Africa. We aimed to assess the burden of malaria and bacteria in Côte d'Ivoire., Methods: Blood samples from 438 febrile and 346 afebrile people were screened using molecular tools., Results: Plasmodium falciparum was the most common microorganism associated with fever (46.8% in febrile, 23.4% in afebrile people; p < 0.001). Bacteraemia was detected in 21.7% of febrile people and 12.7% of afebrile people (p = 0.001). Streptococcus pneumoniae was the main cause of bacteraemia (7.1% of febrile and 0.6% of afebrile individuals; p < 0.001). Non-typhoidal Salmonella spp. was detected in 4.5% of febrile people and 1.2% of afebrile individuals (p < 0.001). Salmonella enterica Typhi and S. enterica Paratyphi were only detected in febrile subjects (1.4% and 2.1%), as well as Tropheryma whipplei (0.9%), Streptococcus pyogenes (0.7%), and Plasmodium ovale (4.6%). The prevalence in febrile and afebrile people was similar for Staphylococcus aureus (3.6-4.9%), Rickettsia felis (5.5-6.4%), Mansonella perstans (3.0-3.2%), and Plasmodium malariae (1.6-2.3%). Comorbidities were higher in febrile than in afebrile subjects (10.3% versus 5.5%; p = 0.01); 82% involving P. falciparum. All patients co-infected with P. falciparum and S. pneumoniae were febrile whereas 30% of those infected by P. falciparum alone were not (p = 0.02). Among febrile participants, 30.4% with malaria and 54.7% with bacteraemia had received neither antimalarial nor antibiotic therapy., Conclusion: Identification of etiologies of acute febrile diseases in sub-Saharan Africa proposes keys to successful treatment and prevention of infectious diseases. Vaccination campaigns may decrease the morbidity of mono- and co-infections by preventable microorganisms., (Copyright © 2021 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Published
2021
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49. Molecular Evidence of Bacteria in Clothes Lice Collected from Homeless People Living in Shelters in Marseille.

Author

Ly TDA, Amanzougaghene N, Hoang VT, Dao TL, Louni M, Mediannikov O, and Gautret P

Subjects
Animals, France, Host-Pathogen Interactions, Phylogeny, Prevalence, Acinetobacter baumannii isolation & purification, Bartonella quintana isolation & purification, Clothing, Ill-Housed Persons, Phthiraptera microbiology
Abstract

We sought to evidence the presence of emerging bacterial pathogens in clothes lice collected from sheltered homeless individuals from Marseille, France. During the 2013-2018 period, a total of 507 lice were collected from 37 individuals and were processed for molecular analysis. We reported a low prevalence of Bartonella quintana DNA carriage (1.2%). No louse tested positive for Rickettsia sp., Rickettsia prowazekii , Borrelia sp., Anaplasma sp., Yersinia Pestis , or Coxiella burnetii . A comparison with studies conducted before 2013 showed a 17.5-fold reduction in the rate of B. quintana DNA positivity. By contrast, a high prevalence of Acinetobacter species DNA carriage (40.8%), mostly A. baumannii (32.9%), was observed, tending to increase over time. In addition, we detected Acinetobacter ursingii DNA in clothes lice for the first time. Genotypic characterization and antimicrobial susceptibility testing of A. baumannii isolates from clothes lice are needed to assess whether these A. baumannii strains present in lice are similar to those responsible for human infections and harbor mechanisms of resistance against antibiotics.

Published
2020
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50. Complexin in ivermectin resistance in body lice.

Author

Amanzougaghene N, Fenollar F, Nappez C, Ben-Amara A, Decloquement P, Azza S, Bechah Y, Chabrière E, Raoult D, and Mediannikov O

Subjects
Amino Acid Sequence, Animals, Cloning, Molecular, Gene Expression Regulation, Insect Proteins genetics, Insecticides, Lice Infestations drug therapy, Nerve Tissue Proteins genetics, Proteomics, RNA, Messenger genetics, Sequence Analysis, DNA, Insect Proteins metabolism, Insecticide Resistance genetics, Ivermectin, Nerve Tissue Proteins metabolism, Pediculus genetics
Abstract

Ivermectin has emerged as very promising pediculicide, particularly in cases of resistance to commonly used pediculicides. Recently, however, the first field-evolved ivermectin-resistance in lice was reported. To gain insight into the mechanisms underlying ivermectin-resistance, we both looked for mutations in the ivermectin-target site (GluCl) and searched the entire proteome for potential new loci involved in resistance from laboratory susceptible and ivermectin-selected resistant body lice. Polymorphism analysis of cDNA GluCl showed no non-silent mutations. Proteomic analysis identified 22 differentially regulated proteins, of which 13 were upregulated and 9 were downregulated in the resistant strain. We evaluated the correlation between mRNA and protein levels by qRT-PCR and found that the trend in transcriptional variation was consistent with the proteomic changes. Among differentially expressed proteins, a complexin i.e. a neuronal protein which plays a key role in regulating neurotransmitter release, was shown to be the most significantly down-expressed in the ivermectin-resistant lice. Moreover, DNA-mutation analysis revealed that some complexin transcripts from resistant lice gained a premature stop codon, suggesting that this down-expression might be due, in part, to secondary effects of a nonsense mutation inside the gene. We further confirmed the association between complexin and ivermectin-resistance by RNA-interfering and found that knocking down the complexin expression induces resistance to ivermectin in susceptible lice. Our results provide evidence that complexin plays a significant role in regulating ivermectin resistance in body lice and represents the first evidence that links complexin to insecticide resistance., Competing Interests: The authors have declared that no competing interests exist.

Published
2018
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