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Objective: To assess the prevalence and intensity of intestinal schistosomiasis and soil transmitted helminthiasis among primary school children in Gorgora, Northwest Ethiopia. Methods: A cross-sectional epidemiological study was carried out in Gorgora primary school in Northwest Ethiopia from November to December 201 0. Stool sample was collected from 326 children (170 male and 156 female) and examined by Kato-Katz technique for intestinal schistosomiasis and soil transmitted helminth infection. Results: A total of 120 children (56 males and 64 females) were positive for single or multiple parasitic infection. Four helminth parasite species were identified and the most prevalent was Ascaris lumbricoides (A. lumbricoides) (16.60%), followed by Schistosoma mansoni (S. mansoni) (10.10%), hookworm (5.50%) and Trichuris trichiura (T. trichiura) (4.60%). Furthermore, 1.84%, 2.76% and 2.45% of the study participants were coinfected with Hookworm & A. lumbricoides, A. lumbricoides & S. mansoni and A. lumbricoides & T. trichiura, respectively. Conclusions: The high prevalence of intestinal schistosomiasis and soil-transmitted helminthiasis observed in the study area shows the need of integrated control programme including periodic deworming, improving sanitation and access to clean water, appropriate health education and environmental measures to have a lasting impact on transmission.

A bacteriocin-producing strain isolated from Ethiopian honey was identified as Enterococcus faecium. The incidence of multidrug resistance has become a leading challenge in the treatment of deadly infectious diseases specifically of bacterial origin. In order to reduce the risk and spread of bacterial antibiotic resistance, various new drugs have been searched and introduced in the biomedical science. In this reference, bacteriocin could have been an important alternative for the control of certain infectious agents. The present study was carried out to check the effect of bacteriocin produced from Enterococcus faecium on multidrug resistant bacterial isolates. The isolates of Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, were isolated from different clinical specimens and were screened for multidrug resistance by the standard Kirby-Bauer disk diffusion method. Bacteriocin was produced from Enterococcus faecium during growth in MRS broth at 37°C and was checked for antibacterial activity against above mentioned resistant bacterial isolates by agar well diffusion method. All the clinical isolates were confirmed to be multi drug resistant. The bacteriocin proved to be active against a narrow range of gram positive bacteria i.e. isolates of Staphylococcus aureus and it showed reduced activity against; isolates of Pseudomonas aeruginosa and Escherichia coli.

Background In Ethiopia, light microscopy is the gold standard for malaria diagnosis although it is not available in most peripheral health facilities. It is time consuming, requires trained personnel and needs careful preparation and application of reagents to ensure quality results. This study was aimed at testing the diagnostic performance of CareStart™ malaria rapid diagnostic test (RDT) with reference to light microscopy for the diagnosis of falciparum and vivax malaria in Ethiopia. Methods Blood samples were collected from 254 patients suspected to have malaria at Kola Diba Health Center in the late malaria transmission peak season from November 2011 to December 2011. The samples were examined immediately by light microscopy and the RDT (CareStart™ Malaria HRP2/pLDH COMBO Test kit). Statistical analysis was performed using SPSS version 16 and the JavaStat two-way contingency table analysis. Results The overall sensitivity and specificity of CareStartTM RDT was found to be 95% (90–97.9%, 95% CI) and 94.2% (90.9–96%, 95% CI), respectively. The sensitivity of the CareStartTM RDT for Plasmodium falciparum or mixed infection was calculated to be 92.9% (82.5–98%, 95%CI) while a sensitivity of 90.9% (74.1–98.4%, 95%CI) was found for non-falciparum species. The specificity for P. falciparum or mixed infections was found to be 95.4% (92.5–96.8%, 95%CI) while it was 97.3% (94.8–98.4%, 95%CI) for non-falciparum species. There was an excellent agreement between the two tests with a kappa value of 0.918. Conclusion The CareStartTM RDT test showed good sensitivity and specificity with an excellent agreement to the reference light microscopy. The RDT could therefore be used in place of light microscopy, which in poor set-ups cannot be used routinely.