29 results on '"Amavet, Patricia Susana"'
Search Results
2. Immuno-endocrine alterations and oxidative stress induced by commercial formulations of pesticides in the tegu lizard (Salvator merianae)
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Mestre, Ana Paula, Odetti, Lucía Magdalena, Marcó, María Virginia Parachú, Poletta, Gisela Laura, Amavet, Patricia Susana, and Siroski, Pablo Ariel
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- 2023
- Full Text
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3. Hybridization and Speciation Among New-World Crocodilian Species
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Pacheco-Sierra, Gualberto, Amavet, Patricia Susana, Zucoloto, Rodrigo Barban, editor, Amavet, Patricia Susana, editor, Verdade, Luciano Martins, editor, and Farias, Izeni Pires, editor
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- 2021
- Full Text
- View/download PDF
4. Genetic Diversity of New World Crocodilians
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Amavet, Patricia Susana, Zucoloto, Rodrigo Barban, Hrbek, Tomas, Farias, Izeni Pires, Zucoloto, Rodrigo Barban, editor, Amavet, Patricia Susana, editor, Verdade, Luciano Martins, editor, and Farias, Izeni Pires, editor
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- 2021
- Full Text
- View/download PDF
5. Molecular Markers Applied to Conservation Genetics of American Crocodilians
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Zucoloto, Rodrigo Barban, Farias, Izeni Pires, Amavet, Patricia Susana, Zucoloto, Rodrigo Barban, editor, Amavet, Patricia Susana, editor, Verdade, Luciano Martins, editor, and Farias, Izeni Pires, editor
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- 2021
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6. Perspectives and Final Considerations About the Molecular Ecology of New-World Crocodilians
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Amavet, Patricia Susana, Zucoloto, Rodrigo Barban, Zucoloto, Rodrigo Barban, editor, Amavet, Patricia Susana, editor, Verdade, Luciano Martins, editor, and Farias, Izeni Pires, editor
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- 2021
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- View/download PDF
7. Perspectives and Final Considerations About the Molecular Ecology of New-World Crocodilians
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Amavet, Patricia Susana, primary and Zucoloto, Rodrigo Barban, additional
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- 2020
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- View/download PDF
8. Molecular Markers Applied to Conservation Genetics of American Crocodilians
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Zucoloto, Rodrigo Barban, primary, Farias, Izeni Pires, additional, and Amavet, Patricia Susana, additional
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- 2020
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- View/download PDF
9. Hybridization and Speciation Among New-World Crocodilian Species
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Pacheco-Sierra, Gualberto, primary and Amavet, Patricia Susana, additional
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- 2020
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10. Effects of glyphosate, cypermethrin, and chlorpyrifos on hematological parameters of the tegu lizard (Salvator merianae) in different embryo stages
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Mestre, Ana Paula, primary, Amavet, Patricia Susana, additional, van der Sloot, Imreël Stefanus, additional, Carletti, Julieta Verónica, additional, Poletta, Gisela Laura, additional, and Siroski, Pablo Ariel, additional
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- 2020
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11. Baseline values of immunologic parameters in the lizard Salvator merianae (Teiidae, squamata)
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Mestre, Ana Paula, Amavet, Patricia Susana, and Siroski, Pablo Ariel
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Ciencias Veterinarias ,Biomarkers, Immune system, Reptilian, Salvator merianae, Sentinel model ,Reptilian ,Immune system ,QL1-991 ,CIENCIAS AGRÍCOLAS ,Otras Ciencias Veterinarias ,Immune System ,Salvator Merianae ,Original Article ,Sentinel model ,Sentinel Model ,purl.org/becyt/ford/4.3 [https] ,Zoology ,Biomarkers ,purl.org/becyt/ford/4 [https] ,Salvator merianae - Abstract
The genus Salvator is widely distributed throughout South America. In Argentina, the species most abundant widely distributed is Salvator merianae. Particularly in Santa Fe province, the area occupied by populations of these lizards overlaps with areas where agriculture was extended. With the aim of established baseline values for four immunologic biomarkers widely used, 36 tegu lizards were evaluated tacking into account different age classes and both sexes. Total leukocyte counts were not different between age classes. Of the leucocytes count, eosinophils levels were higher in neonates compared with juvenile and adults; nevertheless, the heterophils group was the most prevalent leukocyte in the peripheral blood in all age classes. Lymphocytes, monocytes, heterophils, azurophils and basophils levels did not differ with age. Natural antibodies titres were higher in the adults compared with neonates and juveniles lizards. Lastly, complement system activity was low in neonates compared with juveniles and adults. Statistical analysis within each age group showed that gender was not a factor in the outcomes. Based on the results, we concluded that S. merianae demonstrated age (but not gender) related differences in the immune parameters analyzed. Having established baseline values for these four widely-used immunologic biomarkers, ongoing studies will seek to optimize the use of the S. merianae model in future research. Fil: Mestre, Ana Paula. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Ciencias Naturales. Laboratorio de Zoología Aplicada: Anexo Vertebrados (FHUC-UNL/MASPyMA); Argentina. Laboratorio de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Amavet, Patricia Susana. Laboratorio de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Siroski, Pablo Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Limnología "Dr. Raúl A. Ringuelet". Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Limnología; Argentina. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Ciencias Naturales. Laboratorio de Zoología Aplicada: Anexo Vertebrados (FHUC-UNL/MASPyMA); Argentina
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- 2017
12. Phenotypic variability and heritability of the cephalic region of Caiman latirostris
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Imhoff, Carolina Guadalupe, Giri, Federico, Siroski, Pablo Ariel, and Amavet, Patricia Susana
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Ciencias Biológicas ,BROAD-SNOUTED CAIMAN ,PHENOTYPIC VARIABILITY ,Otras Ciencias Biológicas ,MORPHOLOGY ,QUANTITATIVE GENETICS ,CIENCIAS NATURALES Y EXACTAS - Abstract
The study of the cephalic shape of crocodilian is relevant in the fields of ecology, systematics, evolution, and conservation. Therefore, the integration of geometric analysis within quantitative genetics allows the evaluation of the inheritable shape components. In this study, the dorsal cephalic region of 210 Caiman latirostris hatchlings was analyzed from seven populations in Santa Fe, Argentina, to detect intra-, and inter-population phenotypic variability, and to determine the heritability of biological shape and size, using newly available geometric morphometric tools. The principal component analysis showed two configurations of cephalic shape that could be related to sexual dimorphism. In the canonical variate analysis, Procrustes distances between groups indicated that there are differences in shape among populations. Furthermore, the method of partial least squares indicated a covariation between cephalic shape and environmental variables. Regarding to CS of the skull we found significant differences among populations, moreover the partial least squares was also significant. Estimates of the heritability of shape and size were high, indicating that the components of these features are susceptible to the selection. Fil: Imhoff, Carolina Guadalupe. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Ciencias Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Giri, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina Fil: Siroski, Pablo Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Amavet, Patricia Susana. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Ciencias Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
- Published
- 2016
13. Characterization of the Genetic Diversity of Trachemys dorbigni and Phrynops hilarii
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Guidetti, Brenda Yamile, Siroski, Pablo Ariel, and Amavet, Patricia Susana
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Ciencias Biológicas ,molecular markers ,variability ,RAPD ,Otras Ciencias Biológicas ,ISSR ,fresh water turtles ,CIENCIAS NATURALES Y EXACTAS - Abstract
The utilization of RAPD and ISSR molecular markers is proposed to initiate studies of genetic variability in Phrynops hilarii (Chelidae) and Trachemys dorbigni (Emydidae), two species of fresh water turtles distributed in South America. Three primers of RAPD and four of ISSR were selected and the amplified products of these markers were evaluated by electrophoretic runs in agarose and polyacrylamide gels. The levels of heterozygosity, Shannon index and different allele numbers were slightly higher in P. hilarii for both types of markers. Levels of polymorphism were also higher in P. hilarii than T. dorbigni and both were elevated compared to those recorded for other species. The fact that similar results were obtained with both types of markers for all estimates of diversity highlights the usefulness and validity of the RAPD technique. The molecular markers used were found potentially useful for analysing future temporal and spatial distribution of genetic diversity in both species, expanding scales work. Fil: Guidetti, Brenda Yamile. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución. Grupo de Investigación en Ecología de Comunidades del Desierto; Argentina Fil: Siroski, Pablo Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Amavet, Patricia Susana. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Ciencias Naturales; Argentina
- Published
- 2015
14. The broad-snouted caiman population recovery in Argentina. A case of genetics conservation
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Amavet, Patricia Susana, primary, Rueda, Eva Carolina, additional, Vilardi, Juan César, additional, Siroski, Pablo, additional, Larriera, Alejandro, additional, and Saidman, Beatriz Ofelia, additional
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- 2017
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15. Characterization of genetic diversity of Trachemys dorbigni and Phrynops hilarii
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Guidetti, Brenda Yamile, Siroski, Pablo Ariel, and Amavet, Patricia Susana
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Ciencias Biológicas ,VARIABILITY ,RAPD ,ISSR ,MOLECULAR MARKERS ,FRESH WATER TURTLES ,CIENCIAS NATURALES Y EXACTAS ,Conservación de la Biodiversidad - Abstract
The utilization of RAPD and ISSR molecular markers is proposed to initiate studies of genetic variability in Phrynops hilarii and Trachemys dorbigni, two species of fresh water turtles distributed in South America. Three primers of RAPD and four of ISSR were selected and the amplified products of these markers were evaluated by electrophoretic runs in agarose and polyacrylamide gels. The levels of heterozygosity, Shannon index and different allele numbers were slightly higher in P. hilarii for both types of markers. Levels of polymorphism were also higher in P. hilarii than T. dorbigni and both were elevated compared to those recorded for other species. The fact that similar results were obtained with both types of markers for all estimates of diversity highlights the usefulness and validity of the RAPD technique. The molecular markers used were found potentially useful for analysing future temporal and spatial distribution of genetic diversity in both species, expanding scales work. Fil: Guidetti, Brenda Yamile. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Ciencias Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina Fil: Siroski, Pablo Ariel. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Cs.naturales. Laboratorio de Zoología Aplicada: Anexo Vertebrados (fhuc-unl/maspyma); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina Fil: Amavet, Patricia Susana. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Ciencias Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
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- 2015
16. Reptiles as Animal Models: Examples of their Utility in Genetics, Immunology and Toxicology
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Poletta, Gisela Laura, Siroski, Pablo Ariel, Amavet, Patricia Susana, Ortega, Hugo Hector, Mudry, Marta Dolores, and Lutterschmidt, William
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complemento ,Ciencias Biológicas ,Reptiles ,toxicologia ,CIENCIAS NATURALES Y EXACTAS ,Conservación de la Biodiversidad - Abstract
Historically, animals used as experimental models have contributed to the knowledgeof multiple aspects of organisms? biology and wildlife, providing valuable informationabout physiological processes, events, environmental situations, and even humaninteractions. Alternatives to animal testing are primarily based on biochemical assays orexperiments with cells/organs cultures, typically far more sophisticated and specific thanin vivo approaches. However, the whole organism allows for inferences about particularspecies and its situation in natural habitats. Sometimes, it is not possible to study directlythe species of interest, making it necessary to identify the closest related species that canbe used as a model organism. Reptiles may be good and interesting models as theyrespond both behaviorally and physiologically to environmental or experimentalconditions. This chapter specifically describes the utility of crocodiles, lizards, and turtlesas animal models in studies of genetics, immunology, and toxicology. The increasedinterest in reptile genomics is evident by newly sequenced genomes, by the establishmentof significant genomic resources for some reptile groups, and by the awareness thatgenomic diversity in Reptiles is substantially greater than that of mammals. Reptiles alsodemonstrate immune components with an apparently higher activity than othervertebrates. Their ability to resist serious injuries makes them interesting models toelucidate mechanisms within the defense system. In the same way, interesting studieswere performed to propose immune components to be used as indicators of toxicsexposure. Environmental contaminants can significantly affect many reptiles. However,these species are often excluded from toxicology studies and ecological risk assessments,even though they are important elements of ecosystems and show similar sensitivity tothat reported for birds and mammals. Genotoxicity, immunotoxicity and oxidative stresscompounds in reptile species, serving as early-warning signals of populationsenvironmentally exposed. Fil: Poletta, Gisela Laura. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Siroski, Pablo Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Amavet, Patricia Susana. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Mudry, Marta Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
- Published
- 2013
17. Isolation and characterization of new microsatellite markers for application in population genetic studies of Caiman latirostris and related species
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Amavet, Patricia Susana, primary, Rueda, Eva Carolina, additional, Siroski, Pablo Ariel, additional, Larriera, Alejandro, additional, and Saidman, Beatriz Ofelia, additional
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- 2015
- Full Text
- View/download PDF
18. Distribution studies of the genetic variability and analysis of mating system in wild populations of Caiman latirostris (Broad-snouted caiman) (Reptilia, alligatoridae)
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Amavet, Patricia Susana and Saidman, Beatriz
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GENETICA DE POBLACIONES ,CAIMAN LATIROSTRIS ,MICROSATELLITE ,MATING SYSTEM ,ANALISIS DE PATERNIDAD ,VARIABILIDAD ,SISTEMA DE APAREAMIENTO ,MICROSATELITES ,VARIABILITY ,MORFOMETRIA ,ISOENZIMAS ,RAPD ,POPULATION GENETICS ,ISOZYMES ,PATERNITY ANALYSIS ,MORPHOMETRICS - Abstract
Caiman latirostris (yacaré overo) es una de las dos especies de cocodrilianos citadas para nuestro país. Sus poblaciones silvestres son objeto de manejo en Argentina, mediante programas que utilizan el sistema de ranching (cosecha de huevos silvestres para cría en granjas), para su uso sustentable y la conservación de su hábitat. C. latirostris, en nuestro país, se encuentra en el Apéndice II de CITES, el cual permite el comercio regulado de su cuero y carne, y se ha convertido en una especie de gran importancia comercial, a nivel nacional e internacional. Esta tesis incluye estudios genético-poblacionales del yacaré overo en la provincia de Santa Fe, Argentina. Se realizaron análisis de variabilidad, diferenciación y estructura genética empleando isoenzimas, marcadores RAPD y caracteres morfométricos, y un estudio de paternidad utilizando marcadores microsatélites. Los resultados obtenidos indican que las poblaciones analizadas de yacaré overo poseen valores de variabilidad genética de bajos a intermedios, diferenciación poblacional significativa y variabilidad fenotípica alta para algunos de los rasgos morfométricos estudiados. Además, se hallaron indicios de que el sistema de apareamiento de C. latirostris podría incluir el comportamiento de múltiple paternidad, al detectar más de un progenitor paterno en al menos una de las familias analizadas. Caiman latirostris (broad-snouted caiman) is one of the two crocodilian species cited for Argentina. Their wild populations are subject to management in our country, by means of programs that use ranching system (harvest of wild eggs, captive rearing and reintroduction to wild) for their sustainable use and habitat conservation. In Argentina, C. latirostris is included in the Appendix II of CITES, therefore, the regulated trade of meat and leather is allowed, and this species has turned into an important resource at both national and international levels. This thesis includes genetic population studies about broad-snouted caiman in Santa Fe province, Argentina. Analyses related to variability, differentiation and genetic structure were carried out through isozyme electrophoresis, RAPD markers, and quantitative traits. On the other hand, paternity studies were conducted using microsatellite markers. The obtained results indicate that analyzed broad-snouted caiman populations have low to intermediate genetic variability values, significant population differentiation, and high phenotypic variability for some of the studied morphometric traits. In addition, we found indications that C. latirostris mating system could include multiple paternity behavior, since we found more than one paternal progenitor in at least one of the analyzed families. Fil: Amavet, Patricia Susana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
- Published
- 2009
19. Estudios genético-poblacionales de Caiman latirostris (Reptilia, Alligatoridae) en Santa Fe, Argentina: una revisión a través del tiempo
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Amavet, Patricia Susana, Rosso, Daniel Esteban, Markariani, Rosa, and Larriera, Alejandro
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purl.org/becyt/ford/1 [https] ,Ciencias Biológicas ,GENÉTICA ,VARIABILIDAD ,CAIMÁN ,purl.org/becyt/ford/1.6 [https] ,CIENCIAS NATURALES Y EXACTAS ,Conservación de la Biodiversidad - Abstract
Los estudios genéticos en Caiman latirostris (Yacaré overo) en Santa Fe, Argentina, comenzaron con un análisis citogenético en comparación con Caiman yacare. El cariotipo en ambas especies consistió de 42 cromosomas, con un patrón de bandas C difuso y un solo par de cromosomas con NOR. Debido a la falta de diferencias claras entre ambos cariotipos, decidimos usar marcadores moleculares en el análisis de esta especie. Cuatro isoenzimas: Esterasas, Isocitrato Dehidrogenasa, Malato Dehidrogenasa, y Superóxido Dismutasa se analizaron en animales provenientes de cuatro poblaciones santafesinas. En todos los casos se encontraron valores nulos de heterocigosis. Se analizaron 7 primers para RAPD y sólo 13.73 % de 233 marcadores analizados resultaron polimórficos. Los resultados para polimorfismos, heterocigosis y número medio de alelos por locus en las poblaciones fueron niveles bajos a intermedios. El análisis de AMOVA indicó que casi toda la variación existe dentro de las poblaciones, significando que varios alelos son compartidos entre las poblaciones. Debido a que los marcadores RAPD pueden ser menos eficaces en detectar variaciones que los microsatélites en poblaciones de cocodrilianos, en estos momentos estamos comenzando a utilizar esta técnica en el análisis poblacional. Hasta el momento se han amplificado positivamente cuatro primers, y detectamos indicios de la existencia de más de un padre en tres familias. Los datos genéticos de especies nativas son fundamentales para establecer y evaluar planes de manejo, y el análisis de variabilidad es básico para el conocimiento biológico de las especies, aportando a la sistemática, la ecología y la biodiversidad. Fil: Amavet, Patricia Susana. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rosso, Daniel Esteban. Universidad Nacional del Litoral; Argentina Fil: Markariani, Rosa. Universidad Nacional del Litoral; Argentina Fil: Larriera, Alejandro. Universidad Nacional del Litoral; Argentina
- Published
- 2008
20. Mating system and population analysis of the broad-snouted caiman (Caiman latirostris) using microsatellite markers.
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Amavet, Patricia Susana, César Vilardi, Juan, Rueda, Eva Carolina, Larriera, Alejandro, and Saidman, Beatriz Ofelia
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BROAD-nosed caiman , *REPTILE reproduction , *SPECIES diversity , *MICROSATELLITE repeats , *REPTILE populations , *ANIMAL sexual behavior - Abstract
The knowledge about reproductive strategies of a species contributes to their conservation. Multiple paternity is a reproductive behavior increasing effective population size, which could increase genetic diversity particularly in populations submitted to bottlenecks events. In Argentina, wild populations of Caiman latirostris are subject of a management plan devoted to their preservation and sustainable utilization based on its commercial interest. This program started in response to the evident numeric reduction of the populations, as a consequence of hunting pressure and habitat modification; it had a remarkable success in population recovery allowing the commercial use of C. latirostris. Data on reproductive behavior of C. latirostris are limited because mating occurs in the water and the information about their genetic diversity is scarce too. Our specific aims were to study the mating system and population genetic structure applying microsatellite markers in twelve C. latirostris families. The obtained results showed highly significant difference among populations and a lack of correspondence between geographical distance and genetic differentiation suggesting that populations of C. latirostris represent unstable metapopulations. In the paternity analysis was detected more than one father in two nests, which could be explained by capacity of storage sperm, proposed in females of a related species. The behavior of multipaternity could contribute to maintain viable populations of C. latirostris, since the maintenance of genetic variability within populations could help increase their capacity to respond to selective pressure. Further studies employing genetic and behavioral framework are needed to better understand the reproductive biology of C. latirostris. [ABSTRACT FROM AUTHOR]
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- 2017
- Full Text
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21. Mating system and population analysis of the broad-snouted caiman (Caiman latirostris) using microsatellite markers
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Amavet, Patricia Susana, primary, Vilardi, Juan César, additional, Rueda, Eva Carolina, additional, Larriera, Alejandro, additional, and Saidman, Beatriz Ofelia, additional
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- 2012
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22. Crocodilian Genome Advances
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Brittain, Katherine, Ray, David A., Gongora, Jaime, Zucoloto, Rodrigo Barban, editor, Amavet, Patricia Susana, editor, Verdade, Luciano Martins, editor, and Farias, Izeni Pires, editor
- Published
- 2021
- Full Text
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23. How Genetic Tools Can Help Crocodilians’ Management and Governance
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Verdade, Luciano M., Piña, Carlos I., Simoncini, Melina, Silva-Brandão, Karina L., Zucoloto, Rodrigo Barban, editor, Amavet, Patricia Susana, editor, Verdade, Luciano Martins, editor, and Farias, Izeni Pires, editor
- Published
- 2021
- Full Text
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24. Crocodilians Are Promiscuous But Not to the Benefit of Heterozygosity
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Isberg, Sally R., Zucoloto, Rodrigo Barban, editor, Amavet, Patricia Susana, editor, Verdade, Luciano Martins, editor, and Farias, Izeni Pires, editor
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- 2021
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25. Biogeography and Comparative Phylogeography of New-World Crocodylians
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Muniz, Fábio de Lima, Bittencourt, Pedro Senna, Hernández-Rangel, Sandra Marcela, Roberto, Igor Joventino, Farias, Izeni Pires, Hrbek, Tomas, Zucoloto, Rodrigo Barban, editor, Amavet, Patricia Susana, editor, Verdade, Luciano Martins, editor, and Farias, Izeni Pires, editor
- Published
- 2021
- Full Text
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26. Molecular Phylogenetics of the New-World Crocodylia
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Densmore III, Llewellyn D., Hrbek, Tomas, Zucoloto, Rodrigo Barban, editor, Amavet, Patricia Susana, editor, Verdade, Luciano Martins, editor, and Farias, Izeni Pires, editor
- Published
- 2021
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27. Geographic Distribution, Habitat, Reproduction, and Conservation Status of Crocodilians in the Americas
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Villamarín, Francisco, Escobedo-Galván, Armando H., Siroski, Pablo, Magnusson, William E., Zucoloto, Rodrigo Barban, editor, Amavet, Patricia Susana, editor, Verdade, Luciano Martins, editor, and Farias, Izeni Pires, editor
- Published
- 2021
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28. Immunotoxic effect evaluation of pesticides, for agricultural use, in Salvator merianae (iguana overa) as a biomonitor of environmental pollution
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Mestre, Ana Paula, Siroski, Pablo Ariel, Vigliano, Fabricio, Perez Coll, Cristina, Rey, Florencia, and Amavet, Patricia Susana
- Subjects
Corticosterona ,Sistema inmune ,Biomarcadores ,Immune system ,Reptiles ,Plaguicidas ,Pesticides ,Corticosterone ,Biomarkers ,Salvator merianae - Abstract
Fil: Mestre, Ana Paula. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina. La expansión de la frontera agrícola ha afectado las poblaciones silvestres animales en Argentina. Particularmente la distribución natural de la iguana overa (Salvator merianae) ha quedado inmersa en áreas expuestas a plaguicidas. El sistema inmune es un excelente indicador de la salud de los organismos, por lo que este trabajo tuvo como objetivo evaluar el efecto de los agroquímicos más utilizados en el país (cipermetrina, glifosato y clorpirifos) y de sus combinaciones, sobre el sistema inmune de S. merianae. Los parámetros inmunológicos evaluados fueron: recuento total y diferencial de glóbulos blancos, índices de lobularidad y de heterófilos/linfocitos, anticuerpos naturales y actividad del sistema de complemento. Complementariamente, se evaluaron los niveles de corticosterona en sangre y el crecimiento corporal de los individuos. Los resultados sugieren que los plaguicidas aplicados en menores concentraciones que las utilizadas en la agricultura actual, pueden inducir alteraciones en el sistema inmune de embriones y juveniles de S. merianae. Las diferentes respuestas inmunológicas, endócrinas y/o de crecimiento dependieron de las características del formulado, de la marca comercial probada y el método de exposición: individual o combinado, reconociendo interacciones entre los componentes de las mezclas. En animales expuestos durante el desarrollo embrionario, los efectos también dependieron de las diferentes concentraciones y de la etapa embrionaria de los individuos al momento de la exposición. En base a los resultados obtenidos, se sugiere continuar con la evaluación de los efectos de plaguicidas, empleando biomarcadores que permitan identificar las condiciones reales de exposición en las que se encuentra esta especie en su ambiente natural. The expansion of the agricultural frontier in recent years has excessively affected wild animal populations in Argentina. Particularly, the natural distribution of the Tegu Lizard (Salvator merianae) has been immersed in areas exposed to different pesticide formulations. The immune system is an excellent indicator of the health of organisms, so in this Doctoral Thesis, we begin to evaluate the possible effect of the most widely used agrochemicals in the country (cypermethrin, glyphosate, and chlorpyrifos) and their mixtures, on the immune system of S. merianae. The immunological parameters evaluated were: total and differential white blood cell count, lobularity index, heterophils / lymphocytes index, natural antibodies titres, and complement system activity. Besides, corticosterone levels and body growth of individuals were evaluated. The results showed that the formulations tested at lower concentrations to those recommended for crops application, induce immune system alterations of embryos and juveniles S. merianae exposed. Different immune, endocrine, and / or body growth organism’s responses to pesticide exposures, changed according to the compound applied, the glyphosate trademark tested, and the exposure method: individual or combined, recognizing different interactions among components in the mixtures. Finally, in animals exposed during embryonic development, toxic effects also changed according to different tested pesticide concentrations and embryonic stage at the time of topical application. Based on these findings on the results, it is suggested to continue with the evaluation of pesticides effects, through the use of biomarkers to identify the real exposure conditions of this species in its natural environment. Consejo Nacional de Investigaciones Científicas y Técnicas
- Published
- 2021
29. Baseline values of immunologic parameters in the lizard Salvator merianae ( Teiidae , Squamata).
- Author
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Mestre AP, Amavet PS, and Siroski PA
- Abstract
The genus Salvator is widely distributed throughout South America. In Argentina, the species most abundant widely distributed is Salvator merianae . Particularly in Santa Fe province, the area occupied by populations of these lizards overlaps with areas where agriculture was extended. With the aim of established baseline values for four immunologic biomarkers widely used, 36 tegu lizards were evaluated tacking into account different age classes and both sexes. Total leukocyte counts were not different between age classes. Of the leucocytes count, eosinophils levels were higher in neonates compared with juvenile and adults; nevertheless, the heterophils group was the most prevalent leukocyte in the peripheral blood in all age classes. Lymphocytes, monocytes, heterophils, azurophils and basophils levels did not differ with age. Natural antibodies titres were higher in the adults compared with neonates and juveniles lizards. Lastly, complement system activity was low in neonates compared with juveniles and adults. Statistical analysis within each age group showed that gender was not a factor in the outcomes. Based on the results, we concluded that S. merianae demonstrated age (but not gender) related differences in the immune parameters analyzed. Having established baseline values for these four widely-used immunologic biomarkers, ongoing studies will seek to optimize the use of the S. merianae model in future research.
- Published
- 2017
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