Search

A protein isolate was prepared from karanj seed (KPI) with 921.2 g protein/kg seed, which contained a negligible amount of anti‐nutritional factors and a balanced amino acid composition, especially rich in methionine. For 60‐day feeding trial, five isonitrogenous (300 g/kg CP) and isocaloric (15 MJ DE/kg) diets were formulated by replacing soybean protein isolate (SPI) on protein equivalent basis, KPI‐0 (control, 0 g/kg KPI); KPI‐25 (replacing 250 g/kg SPI protein with KPI); KPI‐50 (replacing 500 g/kg SPI protein with KPI); KPI‐75 (replacing 750 g/kg SPI protein with KPI) and KPI‐100 (replacing 1,000 g/kg SPI protein with KPI) for the feeding of L. rohita. The weight gain percentage, specific growth rate, feed conversion ratio and protein efficiency ratio were not significantly (p > .05) varied among the KPI fed and control groups. A significantly higher hepatosomatic index was recorded in the control and KPI‐25 groups compared with other groups. The whole‐body compositions, except ether extract, did not differ significantly (p > .05) among the groups. Digestive (amylase, protease, lipase and alkaline phosphatase) and metabolic enzyme activities (hexokinase, transaminases and lactate dehydrogenase) and glycogen stores were not significantly affected, whereas intestinal alkaline phosphatase differed significantly (p

A protein isolate was prepared from the neem seed cake, a by-product of the oil extraction processing industry. With this, the study aimed to assess the effects of dietary neem seed protein isolate (NPI) on growth, nutrient utilization, physio-metabolic responses, and hepatic IGF-I expression of Labeo rohita fingerlings. Five isonitrogenous (270 g/kg crude protein as fed basis) and isocaloric (3800 kcal gross energy/kg as fed basis) experimental diets were formulated with graded level of NPI by replacing soy protein isolate (SPI) on protein equivalent basis. Thus experimental diets viz., control (0 g/kg NPI, 200 g/kg SPI protein), NPI-25 (56 g/kg NPI in replacement of 250 g/kg SPI protein), NPI-50 (110 g/kg NPI in replacement of 500 g/kg SPI protein), NPI-75 (164 g/kg NPI in replacement of 750 g/kg SPI protein), NPI-100 (217 g/kg NPI in replacement of 1000 g/kg SPI protein) were prepared for conducting the feeding trial. One hundred eighty acclimated Labeo rohita fingerlings (3.5. ± .0.2 g mean weight) were equally assigned to five experimental groups in triplicates following the completely randomized design. Fishes were maintained and fed twice daily to the satiation level during the experimental period of 60 days. The study results showed that percent weight gain, protein efficiency ratio, and feed conversion ratio were significantly (P 0.05) different from the treatment groups except for NPI-100, which showed a significant (P 0.05) among the experimental groups. A significantly (P 0.05) growth performances.

Hypoxia in aquatic environment is often a recurrent stressor which can affect growth and health of fishes. However, nutritional intervention can help aquatic animals to cope up with environmental stressors so as to alleviate the adverse effects. Here, we examined the impact of L-Arginine (Arg) supplementation on hypoxia-induced changes in immune responses and resistance to Aeromonas hydrophila infection in Indian Major Carp, Cirrhinus mrigala. The fish were fed with graded levels of Arg for 60 days and exposed to 72 h sublethal level of hypoxia (0.50 ± 0.16 mg/L dissolved oxygen [DO]). The six treatment groups with three replicates having different levels of Arginine such as N0.0 (0% Arg + Normoxia), H0.0 (0% Arg + Hypoxia), N0.7 (0.70% Arg + Normoxia), H0.7 (0.70% Arg + Hypoxia), N1.4 (1.40% Arg + Normoxia), and H1.4 (1.40% Arg + Hypoxia) were used in the study. Subsequently, the fish of different treatment groups were challenged with A. hydrophila. The hypoxia exposed fish exhibited significantly higher (P

Neem protein isolate (NPI) was prepared from neem seed cake (NSC) resulting in five fold increase of crude protein content, amounting to 82%. The recovery of protein was highest (49%) when the NSC was solubilised at pH 12 for 2h and precipitated at pH 4. Crude fibre, nitrogen free extract and total ash contents were significantly (P