Search

Optimal vitamin D status is very important for reflecting not only bone but overall woman's health. The aim of the study was to determine pharmacokinetic variability of 25-hydroxy vitamin D, to reveal and quantify the most significant factors that affect its variability in the population of healthy non-menopausal women using the population pharmacokinetic (PopPK) approach. The study population consisted of 74 healthy reproductive women aged from 35 to 50 years, without the use of any supplement. A population pharmacokinetics analysis was conducted using a nonlinear mixed-effects model software. A total of 35 factors were assessed: demographic, clinical, biochemical data and lifestyle factors. The average age and bodyweight of our participants were 40.11 ± 4.35 years 65.30 ± 6.80 kg, respectively. The observed mean serum concentration of 25-hydroxy vitamin D was 26.51 ± 13.49 ng/mL with a wide range of 6.97 to 59.89 ng/mL. Development final PopPK model of the clearance of 25-hydroxy vitamin D showed that only the average daily dose of vitamin D intake from food had a significant influence, with a magnitude of its effects of 0.00401. These results could help when individualizing vitamin D intake in the form of supplements, especially during the wintertime, in healthy reproductive women.

The aim of this study was to investigate in vitro antioxidant and antimicrobial potential of methanol and chloroform extracts of Sanguisorba minor L. subsp. muricata Briq. herba (S. minor). Total phenolic and flavonoid content of the investigated extracts were characterized. Antioxidant activity was estimated by five different in vitro assays. Antioxidant potency composite index was calculated also. Antimicrobial activity was tested against nine bacterial and one fungus strains by the micro-well dilution assay. The methanol extract of S. minor contains more phenols and shows the stronger antioxidant and antibacterial activity in comparison to the chloroform extract. However, the chloroform extract was superior to the methanol extract in content of flavonoids. Gram-positive bacteria were more sensitive than Gram-negative, to both extracts, Staphylococcus aureus being the most sensitive. Sanguisorba minor extracts were not considerably active against Candida albicans. The previous research data about Sanguisorba minor are scarce, so this data represent the first report on antimicrobial activity of S. minor. These results indicate that extracts of S. minor subsp. muricata have evidence-based potential for more comprehensive studies.