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La resistencia bacteriana frente a los antibióticos es uno de los problemas más grandes de salud pública a nivel mundial. Esto dificulta controlar las enfermedades infecciosas causadas por las bacterias, ya que genera fallo terapéutico, alarga los tratamientos y aumenta costes en la atención de la salud. Staphylococcus spp, es uno de los grupos bacterianos más estudiados debido a su importancia clínica y las altas tasas de resistencia que presenta frente a los antibióticos. La especie más importantes es Staphylococcus aureus considerándose la más patógena seguida de los estafilococos coagulasa negativos que actúan como patógenos oportunistas. Este es un estudio retrospectivo cuyo objetivo es determinar patrones de resistencia bacteriana de Staphylococcus spp de los registros de antibiogramas realizados en el Laboratorio Clínico Medilab-Medihospital 2018 - 2020. Donde se aislaron 86 cultivos positivos de Staphylococcus aureus con el 71,43% y Staphylococcus epidermidis con el 78,40%, con más frecuente en el área de consulta externa y predominando en muestras de secreciones con el 77,6% y 67,6% de acuerdo a la especie. El perfil de resistencia de Staphylococcus aureus frente a los antibióticos ensayados en el laboratorio de microbiología son: penicilina (87%), oxacilina (81,50%) y eritromicina (64,6%); mientras que el de Staphylococcus epidermidis fue: penicilina (75%), oxacilina (66,7%), eritromicina (62,9%) y clindamicina (52,8%). Se concluye que ambos grupos de microorganismos expresan fenotipos de resistencia a oxacilina, penicilina y eritromicina, sensibilidad a vancomicina y linezolid y susceptibilidad variable al resto de antibióticos evaluados.

This research investigates the behaviour of the Discharge Points in a Municipal Solid Waste Management System to evaluate the feasibility of making individual predictions of every Discharge Point. Such predictions could enhance system management through optimisation, improving their ecological and economic impact. The current approaches consider installations as a whole, but individual predictions may yield better results. This paper follows a methodology that includes analysing data from 200 different Discharge Points over a period of four years and applying twelve forecast algorithms found as more commonly used for these predictions in the literature, including Random Forest, Support Vector Machines, and Decision Tree, to identify predictive patterns. The results are compared and evaluated to determine the accuracy of individual predictions and their potential improvements. As the results show that the algorithms do not capture the individual Discharge Points behaviour, alternative approaches are suggested for further development.

Este trabajo analiza el seguimiento informativo de la campaña de las elecciones andaluzas de 2022 y su vinculación con el interés manifestado por los ciudadanos en la campaña y con su participación a través del voto y como transmisores de contenidos sobre la misma. Para determinar la existencia de relaciones entre las variables consideradas se usa el test de hipótesis chi-cuadrado. La fuerza de las asociaciones se mide con el coeficiente V de Cramer y el coeficiente de contingencia. Los resultados muestran la existencia de asociación entre el interés en la campaña y su seguimiento mediático. La televisión es el medio preferido para informarse, seguido por la prensa digital y la radio. Los debates y las entrevistas son los contenidos más consumidos. La participación mediante conversaciones se lleva a cabo especialmente en entornos familiares. Sin embargo, no se observa relación entre el interés en el seguimiento mediático de la campaña y la participación a través del voto. Incluso aquellas personas que siguieron la campaña con mayor interés parecían tener decidido el voto ya antes de los comicios.

El presente estudio tiene por objetivo analizar la labor profesional que desempeñan los/las Trabajadores/as Sociales durante su inserción en tres establecimientos educativos públicos de enseñanza básica en la Comuna de Copiapó, Región de Atacama, Chile a los 15 años de la implementación de la Ley de Subvención Escolar Preferencial (SEP). Para ello, se desarrolló un estudio de tipo cualitativo con diseño de estudio de casos y elementos etnográficos con la participación de tres profesionales de Trabajo Social que ejercen funciones en establecimientos educativos públicos de enseñanza básica pertenecientes al Servicio Local de Educación Atacama. La recolección de información se realizó mediante el desarrollo de entrevistas semiestructuradas y revisión documental a partir de lo cual se establecieron tres categorías de análisis, concluyendo que si bien no se evidencian lineamientos laborales establecidos que orienten el quehacer profesional, se identifica que los profesionales ejercen un rol importante en este contexto mediante el desarrollo de prácticas educativas implementadas con los actores de sus comunidades.

There is a growing search for methodologies and tools that foster student motivation and learning. This experimental study aims to examine students’ acceptance and attitude towards the use of game-based applications, as well as analyze how game-based learning impacts the acquisition and development of basic competences. To this end, online questionnaire responses from 135 undergraduate computer science students were analyzed using Structural Equation Modeling (SEM). The results indicate that students perceive these tools as useful and easy to use, thus exerting a positive influence on learning motivation and behavior change towards the implementation of game-based learning. Furthermore, the crucial role of game-based learning strategies in the development of formative collaborative learning is emphasized, as well as their positive influence on the acquisition of essential soft skills for the comprehensive training of computer science students. The conclusions underscore the importance of considering the GBL approach as a valuable educational strategy to motivate and enhance learning and develop fundamental skills in students.

The ability of flow cytometry to identify and quantify the presence of cell populations defined by their expression profile of specific markers has made this technique a powerful and routinary tool in clinical diagnostic practice. Specifically in the field of hematological malignancies, flow cytometry allows the identification of the correct type and lineage of each patient’s disease and also sensitively quantifies the presence of the disease at precise moments during treatment, that is, levels of measurable residual disease (MRD). The quantification of MRD by flow cytometry has allowed the adaptation of tailored therapies to patients, contributing to the improvement of the results of the different protocols in recent decades. In this context, our objective in the present work was to evaluate the potential impact that spectral flow cytometry can provide compared to conventional cytometry, which is the one usually used in clinics. We present here a comparative study of both technologies, spectral versus conventional flow cytometry, in primary samples corresponding to the diagnosis and follow-up of children and adolescents with acute lymphoblastic leukemia. Our initial experience demonstrates the feasibility of incorporating spectral flow cytometry into the routine workflow of a reference laboratory.

The success of developing and implementing Smart Cities (SC) projects depends on a varied set of factors, where the availability of a qualified technical workforce is a critical one. The combination of ICT requirements, like the effectiveness and quality of solutions merging IoT, cloud computing, sensors, and communications with the work from many varied disciplines (e.g., civil engineering, architecture, etc.), mixed with aspects of environmental and business sustainability, makes the management of these projects really challenging. Reports forecast a scarcity of qualified candidates, given this complexity and the growth of activity in SC projects. The European project SMACITE has addressed the requirements of the qualification of an ICT workforce with an analysis of multiples sources of information from the labor market, feedback from involved stakeholders, and the literature. The goal was the development of two occupational ICT profiles as a reference for training and for the availability of candidates for job vacancies. The result is two ICT role profiles for engineers and technicians, mapped with the European skills frameworks ESCO and EN16234. The profiles determined the whole set of requirements, including not only the technical areas and soft skills, but also additional technical areas and sustainability and managerial skills and the analysis of different sources of information. Our work has also determined which existing ESCO occupations are similar to the two reference profiles, so they are better adapted to SC projects. The training activities of SMACITE have also suggested the amount of training expected for a varied sample of candidates who want to be qualified for SC projects.

Abstract The widespread use of digital technologies and the expansion of social networks has created new communication and meeting spaces where people and social and political actors connect with each other. This opens diverse spaces and possibilities for digital engagement in a more accessible, immediate, continuous, egalitarian, and personalized way. Digital technology facilitates learning, dissemination, and access to information, turning it into a means of communication and fueling the practice of critical thinking. In particular civic critical thinking practices improve the organization and effectiveness of civic networks and spaces for citizen participation, ultimately helping to produce responsible, conscious citizens. This study proposes a series of hypotheses based on the relationships between digital learning, critical thinking and civic participation, and tests them using the technique of structural equation modeling (SEM) with partial least squares (PLS) applied to a sample of 191 primary and secondary school students. The results indicate that digital tools have a positive impact on the development of critical thinking, and this influences citizen participation, transforming people into more engaged citizens of the world with participatory attitudes and values.

Identifying cryptic species is pivotal for understanding marine biodiversity and optimizing strategies for its conservation. A robust understanding of poriferan diversity is a complex endeavour. It has also been extremely hampered by the high phenotypic plasticity and the limited number of diagnostic characters. Mycale (Carmia) cecilia has different body colours, even among individuals living together. We tested whether the colour variation could be due to polymorphism, phenotypic plasticity or cryptic speciation. Phylogenetic reconstructions of nuclear and mitochondrial loci were congruent. Individuals of different body colour did not cluster together and had high levels of genetic divergence. Furthermore, the green morphotype clustered in almost all reconstructions with Mycale (C.) phyllophila, as both showed higher gene similarity at the transcriptomic level (public transcriptome). Morphologically, the green individuals consistently showed discrepancies from the red ones. These results suggest that all individuals with the same body colour, either red or green, correspond to the same species, while individuals with different body colours probably belong to different species. These results reveal high levels of morphologic and genetic diversity, which could have important implications for what is known as M. (C.) cecilia and the Mycalidae systematics.

IntroductionLoss of B-cell aplasia (BCA) is a well-known marker of functional loss of CD19 CAR-T. Most relapses and loss of BCA occur in the first months after CD19 CAR-T infusion. In addition, high tumor burden (HTB) has shown to have a strong impact on relapse, especially in CD19-negative. However, little is known about the impact of late loss of BCA or the relationship between BCA and pre-infusion tumor burden in patients infused with tisagenlecleucel for relapsed/refractory B-cell acute lymphoblastic leukemia. Therefore, the optimal management of patients with loss of BCA is yet to be defined.MethodsWe conducted a Spanish, multicentre, retrospective study in patients infused with tisagenlecleucel after marketing authorization. A total of 73 consecutively treated patients were evaluated. ResultsPrior to infusion, 39 patients had HTB (≥ 5% bone marrow blasts) whereas 34 had a low tumor burden (LTB) (

Este artículo analiza las publicaciones científicas sobre los influencers en las redes sociales con objeto de explorar su relación en torno a las estrategias de branding. Para ello se lleva a cabo una revisión sistemática de estos conceptos a través de una búsqueda de artículos científicos que contempla las bases de datos Web of Science y Scopus. A partir de una muestra de un total de 74 publicaciones académicas revisadas por pares se advierte que las temáticas que relacionan las estrategias de influencers con el brand management son relativamente escasas. El incipiente crecimiento de los estudios científicos sobre el marketing de influencer unido al empleo de metodologías, en su mayoría de tipo exploratorio, en redes sociales, explican una tendencia centrada en el abordaje de estos perfiles desde una perspectiva mayoritariamente interesada en esclarecer cuestiones relacionadas con la ética, determinar las técnicas persuasivas empleadas por los influencers u observar su relación con los públicos desde una perspectiva social. Se aprecia la necesidad de continuar nutriendo una línea de investigación sólida que vincule el estudio de los influencers a las posibles implicaciones con respecto a las estrategias de marca.

This paper describes the design and methodology for the development and validation of an intelligent model in the healthcare domain. The generated model relies on artificial intelligence techniques, aiming to predict the length of stay and survival rate of patients admitted to a critical care hospitalization unit with better results than predictive systems using scoring. The proposed methodology is based on the following stages: preliminary data analysis, analysis of the architecture and systems integration model, the big data model approach, information structure and process development, and the application of machine learning techniques. This investigation substantiates that automated machine learning models significantly surpass traditional prediction techniques for patient outcomes within critical care settings. Specifically, the machine learning-based model attained an F1 score of 0.351 for mortality forecast and 0.615 for length of stay, in contrast to the traditional scoring model’s F1 scores of 0.112 for mortality and 0.412 for length of stay. These results strongly support the advantages of integrating advanced computational techniques in critical healthcare environments. It is also shown that the use of integration architectures allows for improving the quality of the information by providing a data repository large enough to generate intelligent models. From a clinical point of view, obtaining more accurate results in the estimation of the ICU stay and survival offers the possibility of expanding the uses of the model to the identification and prioritization of patients who are candidates for admission to the ICU, as well as the management of patients with specific conditions.

Remote sensing data can be a powerful and cost-effective method for determining the extent, composition, and structure of ecosystems across large areas. To use this tool for effective conservation of individual species, we need to test the assumption that remotely sensed habitat indices correspond to both patch suitability (i.e., presence) and value (i.e., demographic rates) for the species reliant on those habitats. We built an open pine habitat quality index (HQI) from remotely sensed spectral data to identify the condition of pine stands. We correlated the HQI with presence, group size, and fledgling production of an avian species (red-cockaded woodpecker, Dryobates (=Picoides) borealis, RCW) associated with longleaf pine (Pinus palustris) using Bayesian logistic regressions and structural equation models. The HQI was correlated with RCW cavity tree and foraging area presence, with 10.8 and 16.0 increased odds of RCW presence with each unit increase in the HQI, respectively. However, the HQI was not correlated with RCW demographic performance. Given that RCWs are an umbrella species and are currently limited by habitat, that the HQI successfully identifies areas of higher quality habitat for RCW is useful for conservation planning. Improving the specificity of the remotely sensed index could improve the link between the HQI and RCW demographic performance, but might limit its application to RCW, instead of the entire open pine endemic community. Our study suggests that linking generalized habitat indices to species habitat suitability is possible and strengthens the justification for their use in cost-effective, large-scale conservation of imperiled ecosystems.

The number of mobile apps is continuously growing but development organisations are not sure of the good alignment of the skills of application developers with what the industry needs. A precise definition of the professional profile of the Mobile Application Developer (MAD) can help to better understand the needs of software development teams. This cooperation of several European organisations for analysing professional profile of MAD has led to a study of skills and competences resulting in a conceptual model with relevant characteristics: a) based on literature review, b) framed within the European standard e-Competence Framework, e-CF (EN16234) and the ESCO official labour classification and c) empirically validated with qualitative and quantitative data from many stakeholders in the field. This analysis might help to add homogeneity to talent management overcoming possible barriers for international mobility within EU as the concepts are taken from the EU reference models.

El artículo tiene por objetivo el analizar la cooperación internacional, desde el convenio entre la Universidad de Atacama UDA-Chile y la Universidad de Nariño UDENAR-Colombia, las dos instituciones son de educación superior públicas y regionales. El estudio fue cualitativo - descriptivo, los participantes fueron I3 entre docentes, administrativos y estudiantes pertenecientes a las dos universidades. Sus edades fluctúan entre los 21 y 59 años, hombres y mujeres, nacidos en Chile o Colombia. Se utilizó como técnicas de recolección de información el análisis documental y un cuestionario con preguntas abiertas. Los resultados muestran una valoración positiva del convenio puesto que se ha manejado desde las tres funciones sustantivas universitarias: docencia, investigación e interacción social; en este mismo sentido se hace fuerte los aprendizajes adquiridos por cada participante y la necesidad de profundizar en investigación. Se concluye que la internacionalización tiene muchos beneficios desde el intercambio cultural, el prestigio internacional y los aprendizajes, así como la transdisiciplinariedad del convenio, que a través del pensamiento crítico construyendo espacios de diálogo de saberes no fragmentado en disciplinas, sino unido por un interés genuino por el conocimiento.

Although children and adolescents with acute lymphoblastic leukaemia (ALL) have high survival rates, approximately 15-20% of patients relapse. Risk of relapse is routinely estimated at diagnosis by biological factors, including flow cytometry data. This high-dimensional data is typically manually assessed by projecting it onto a subset of biomarkers. Cell density and "empty spaces" in 2D projections of the data, i.e. regions devoid of cells, are then used for qualitative assessment. Here, we use topological data analysis (TDA), which quantifies shapes, including empty spaces, in data, to analyse pre-treatment ALL datasets with known patient outcomes. We combine these fully unsupervised analyses with Machine Learning (ML) to identify significant shape characteristics and demonstrate that they accurately predict risk of relapse, particularly for patients previously classified as 'low risk'. We independently confirm the predictive power of CD10, CD20, CD38, and CD45 as biomarkers for ALL diagnosis. Based on our analyses, we propose three increasingly detailed prognostic pipelines for analysing flow cytometry data from ALL patients depending on technical and technological availability: 1. Visual inspection of specific biological features in biparametric projections of the data; 2. Computation of quantitative topological descriptors of such projections; 3. A combined analysis, using TDA and ML, in the four-parameter space defined by CD10, CD20, CD38 and CD45. Our analyses readily extend to other haematological malignancies.

Key Clinical Message HHV8‐ and EBV‐negative primary effusion lymphoma is an extremely rare neoplasm involving body cavities without detectable tumor mass. It usually presents in elderly patients without known immunodeficiency. Compared to primary effusion lymphoma, it has a better prognosis. Primary effusion lymphoma (PEL) is a rare non‐Hodgkin lymphoma confined exclusively to body cavities without detectable tumor masses. The term PEL‐like is an entity similar to PEL in clinical presentation but without relation to human herpesvirus 8 (HHV8). We report a case of HHV8‐ and EBV‐negative primary effusion‐based lymphoma.

IntroductionThe use of peripheral blood progenitor cells (PBPCs) as a source for hematopoietic stem cell transplantation (HSCT) in pediatric healthy donors is still under debate. The risk of a central venous catheter (CVC) placement and catheter-related complications continue to be the main arguments to discourage its use.Methodswe present a retrospective analysis of 140 PBPC collections in pediatric patients and donors, describing adverse events (AE) related to CVCs as well as the influence of catheterrelated variables on the efficiency of the leukapheresis.Results14 CVC-related AEs were recorded (10%). The most common was fever in 5 patients, 4 of which had a catheter-related bacteriemia. Thrombotic events were only observed in 3 patients with active malignancy. A healthy donor presented a moderate bleeding after catheter withdrawal that resolved with local measures, and none of the rest presented any AE. Regarding variables related to the development of AEs, the subject group (patient or donor) was the only one significantly associated (p

Abstract Digital counting methods were developed to decrease the high intra- and inter-observer variability of immunohistochemical markers such as Ki67, with most presenting a good correlation coefficient (CC). Since Ki67 is one of the major contributors to Oncotype DX, it is conceivable that Ki67 expression and the recurrence score (RS) obtained by the multigene panel are positively correlated. We decided first to test to what extent conventional and digital Ki67 quantification methods correlate in daily practice and, second, to determine which of these methods correlates better with the prognostic capacity of the Oncotype DX test. Both Ki67 evaluations were performed in 89 core biopsies with a diagnosis of estrogen receptor (ER) positive HER2-negative breast cancer (BC). Cases were, thus, classified twice for surrogate subtype: first by conventional analysis and then by digital evaluation. The Oncotype RS was obtained in 55 cases that were subsequently correlated to Ki67 evaluation by both methods. Conventional and digital Ki67 evaluation showed good concordance and correlation (CC = 0.81 (95% CI 0.73–0.89)). The correlation of Oncotype DX risk groups and surrogate derived subtypes was slightly higher for the digital technique (rs = 0.46, p

El propósito de este artículo es presentar los nuevos desafíos de la práctica docente derivados del análisis del perfil de quienes son protagonistas en el proceso de enseñanza-aprendizaje: estudiantes y docentes. Para ello se utilizó una metodología mixta, con técnicas de observación documental focalizada en la revisión de información secundaria disponible y encuesta al cuerpo académico de la unidad a través de la autoaplicación de un cuestionario online. Se evidencia en estudiantes la disminución de habilidades comunicativas y el aumento de actividad laboral paralela. En docentes se perfila una práctica flexible, comunicativa y que incentiva el trabajo colaborativo. Así, la práctica docente, bajo el modelo constructivista, se modifica en la medida que se presentan nuevas condiciones y escenarios de aprendizaje.

IntroductionA multicenter prospective cohort study studied patients admitted to the intensive care unit (ICU) by coronavirus-19 (COVID-19) with respiratory involvement. We observed the number of occasions in which the value of procalcitonin (PCT) was higher than 0.5 ng/ml.ObjectiveEvaluation of PCT elevation and influence on mortality in patients admitted to the ICU for COVID-19 with respiratory involvement.Measurements and main resultsWe studied 201 patients. On the day of admission, acute physiology and chronic health evaluation (APACHE)-II was 13 (10–16) points. In-hospital mortality was 36.8%. During ICU stay, 104 patients presented 1 or more episodes of PCT elevation and 60 (57.7%) died and 97 patients did not present any episodes of PCT elevation and only 14 (14.4%) died (p < 0.001). Multivariable analysis showed that mortality was associated with APACHE-II: [odds ratio (OR): 1.13 (1.04–1.23)], acute kidney injury [OR: 2.21 (1.1–4.42)] and with the presentation of one or more episodes of escalating PCT: [OR: 5.07 (2.44–10.53)]. Of 71 patients who died, 59.2% had an elevated PCT value on the last day, and of the 124 patients who survived, only 3.2% had an elevated PCT value on the last day (p < 0.001). On the last day of the ICU stay, the sequential organ failure assessment (SOFA) score of those who died was 9 (6–11) and 1 (0–2) points in survivors (p < 0.001). Of the 42 patients who died and in whom PCT was elevated on the last day, 71.4% were considered to have a mainly non-respiratory cause of death.ConclusionIn patients admitted to the ICU by COVID-19 with respiratory involvement, numerous episodes of PCT elevation are observed, related to mortality. PCT was elevated on the last day in more than half of the patients who died. Serial assessment of procalcitonin in these patients is useful because it alerts to situations of high risk of death. This may be useful in the future to improve the treatment and prognosis of these patients.

Olive tree vegetal materials are considered a powerful source for the isolation of bioactive compounds—mainly phenols and triterpenic acids. However, the high humidity content of them reduces their preservation and extractability to a liquid solvent. Accordingly, a drying step is crucial to homogenize the material and to obtain an efficient extraction. We studied the influence of the drying process on the extraction efficiency of bioactive compounds from olive vegetal material. For this purpose, we evaluated the effects of four drying processes on the solid–liquid extraction of bioactive compounds from two by-products, olive leaves and pomace, and olive fruits harvested from two cultivars, Alfafara and Koroneiki. Infrared-assisted drying (IAD) was the most suited approach to obtain extracts enriched in oleuropein from leaves (28.5 and 22.2% dry weight in Alfafara and Koroneiki, respectively). In the case of pomace, lyophilization and microwave-assisted drying led to extracts concentrated in oleacein and oleuropein aglycone, whereas IAD and oven-drying led to extracts with enhanced contents of hydroxytyrosol glucoside and hydroxytyrosol, respectively. The drying process considerably affected the chemical composition of extracts obtained from fruits. Changes in the composition of the extracts were explained essentially by the drying process conditions using auxiliary energies, temperature, and time, which promoted chemical alterations and increased the extractability of the compounds. Therefore, the drying protocol should be selected depending on the phenolic content and initial raw material.

Difficulties in the collection of hematopoietic stem and progenitor cells (HSPCs) from Fanconi anemia (FA) patients have limited the gene therapy in this disease. We have investigated (ClinicalTrials.gov, NCT02931071) the safety and efficacy of filgrastim and plerixafor for mobilization of HSPCs and collection by leukapheresis in FA patients. Nine of eleven enrolled patients mobilized beyond the threshold level of 5 CD34+ cells/μL required to initiate apheresis. A median of 21.8 CD34+ cells/μL was reached at the peak of mobilization. Significantly, the oldest patients (15 and 16 years old) were the only ones who did not reach that threshold. A median of 4.27 million CD34+ cells/kg was collected in 2 or 3 aphereses. These numbers were markedly decreased to 1.1 million CD34+ cells/kg after immunoselection, probably because of weak expression of the CD34 antigen. However, these numbers were sufficient to facilitate the engraftment of corrected HSPCs in non-conditioned patients. No procedure-associated serious adverse events were observed. Mobilization of CD34+ cells correlated with younger age, higher leukocyte counts and hemoglobin values, lower mean corpuscular volume, and higher proportion of CD34+ cells in bone marrow (BM). All these values offer crucial information for the enrollment of FA patients for gene therapy protocols.

BackgroundT-cell depleted (TCD) haploidentical transplantation using CD3+/CD19+ and TCRαβ+/CD19+ depletion techniques has been increasingly used in children with hematological malignancies. We present a retrospective study aimed to compare transplant outcomes in children with leukemia receiving a TCD haploidentical transplant using either CD3+/CD19+ or TCRαβ+/CD19+ platforms.MethodsA total of 159 children with leukemia (ALL=80) (AML=79) that received a TCD haploidentical transplantation using either CD3+/CD19+ (n=79) or TCRαβ+/CD19+ (n=80) platforms between 2005 and 2020 were included. Median age was 9 years in both groups. There were no differences in patient, donor, and transplant characteristics between groups except for donor KIR B genotype more frequent in the TCRαβ+/CD19+ group (91%) than in the CD3+/CD19+ group (76%) (p=0.009) and a high number of NK+ cells and lower CD19+ cells infused in the TCRαβ+/CD19+ group (35.32x106/kg and 0.06 x106/Kg) than in the CD3+/CD19 group (24.6x106/Kg and 0.25 x106/Kg) (p=0.04 and p=0.0001), respectively. Conditioning was based on TBF. Median follow-up for survivors was 11 years (range; 8-16 y) in CD3+/CD19+ group and 5 years (range; 2-9 y) in the TCRαβ+/CD19+ group.ResultsEngraftment kinetics were similar in both groups (13 days for neutrophils and 10 days for platelets). There was no difference in the incidence of acute GvHD II-IV (29 ± 5% in the CD3+/CD19+ group vs 38 ± 5% in the TCRαβ+/CD19+ group) and chronic GvHD (32 ± 5% vs 23 ± 4%, respectively). NRM was 23 ± 5% in the CD3+/CD19+group vs 21 ± 4% in the TCRαβ+/CD19+group. Relapse incidence was also similar, 32 ± 5% vs 34 ± 6%, respectively. DFS and OS were not different (45 ± 5% vs 45 ± 6% and 53 ± 6% vs 58 ± 6% respectively). As there were no differences on transplant outcomes between groups, we further analyzed all patients together for risk factors associated with transplant outcomes. On multivariate analysis, we identified that early disease status at transplant (HR: 0.16; 95%CI (0.07-0.35) (p=0.0001), presence of cGvHD (HR: 0.38; 95%CI (0.20-0.70) (p= 0.002), and donor KIR-B genotype (HR: 0.50; 95%CI (0.32-0.90) (p=0.04) were associated with better DFS.ConclusionsOur data suggest that there are no advantages in transplant outcomes between TCD platforms. Risk factors for survival are dependent on disease characteristic, donor KIR genotype, and chronic GvHD rather than the TCD platform used.

This paper examines the situation of e-commerce and m-commerce in SMEs in the European Union and analyses if their behaviour in this field is determined by a series of factors, such as the size of the company, the type of business, turnover, etc. which may also influence their needs of specific training for their employees. This study is based on an online survey responded by 674 SMEs from eight different countries in Europe, aimed at providing training assistance to SMEs to support their efforts towards m-commerce. The objective is the analysis of the skills and knowledge to be recommended to employees of SMEs for a better preparation to implement the companies' strategy of m-commerce. Although analysis of results from survey has supported the initial goal of developing training courses for m-commerce which include different economic, legal and technical topics, it has mainly provided very relevant conclusions on the factors which impact the adoption of e-commerce and m-commerce by SMEs.

The commonly accepted definition of sustainability considers the availability of relevant resources to make an activity feasible and durable while also recognizing users’ support as an essential part of the social side of sustainability. IoT represents a disruption in the general scenario of computing for both users and professionals. The real expansion and integration of applications based on IoT depend on our capacity of exploring the necessary skills and professional profiles that are essential for the implementation of IoT projects, but also on the perception of relevant aspects for users, e.g., privacy, legal, IPR, and security issues. Our participation in several EU-funded projects with a focus on this area has enabled the collection of information on both sides of IoT sustainability through surveys but also by collecting data from a variety of sources. Thanks to these varied and complementary sources of information, this article will explore the user and professional aspects of the sustainability of the Internet of Things in practice.

Lactococcus garvieae, a gram-positive anaerobe facultative coccus, is a well-known pathogen in the aquaculture and cattle sector, being extremely rare for human beings. There are some case reports of infections caused by this microorganism, however, there are no fasciitis cases up to date. This is a case of a 24-year-old patient with uncontrolled diabetes mellitus and previous COVID-19 pneumonia without sequelae, admitted to the emergency room for a case compatible with fasciitis. Three cultures for L. garvieae were obtained from surgical debridement and microbiological studies were performed using automated VITEK-2 equipment. No posterior complications were documented. The patient went through a skin graft with a favorable response without evidence of clinical relapse.

Resumen: Antecedentes y objetivo: La enfermedad renal crónica (ERC) requiere de un proceso de adaptación en el paciente, que se puede facilitar con el apoyo de los profesionales sanitarios, así como por iguales capacitados. El objetivo de este estudio es presentar la puesta en marcha de un programa piloto de paciente mentor para promover la adaptación de los pacientes con ERC. Materiales y método: Diseño mixto (cuantitativo y cualitativo) pre-post. El estudio se llevó a cabo en 6 hospitales de España. Los instrumentos utilizados para medir el impacto fueron escalas elaboradas ad-hoc (formato de respuesta escala de Likert de 10 puntos) de satisfacción y adquisición de competencias, así como la creación de grupos focales con 8 pacientes mentores y 10 profesionales sanitarios. Se dividió el programa en 4 fases: 1) Diseño y validación de contenidos del programa manualizado y selección de pacientes mentores; 2) Formación a mentores, satisfacción con la formación y competencias adquiridas por los mentores; 3) Implementación de los grupos de apoyo mutuo y perfil de los asistentes a los grupos de apoyo mutuo, y 4) Evaluación y resultados del programa de Mentoring. Resultados: Se han formado a un total de 39 mentores en habilidades para conducción de grupos, así como para facilitar apoyo emocional. Se han conducido 22 grupos de apoyo con 121 participantes (22% cuidadores). El 65% de los pacientes estaban en consulta de ERC. Un 65% de los pacientes participantes consideraron hacer algún cambio en su estilo de vida tras la asistencia al programa. Todos los ítems que evalúan satisfacción y utilidad han mostrado una puntuación muy elevada, por encima del valor 8,5 sobre 10. Conclusiones: Este es el primer programa manualizado de Mentoring en ERC llevado a cabo de manera simultánea en 6 hospitales españoles. La naturaleza del programa, manualizado y altamente estructurado, permite su replicabilidad minimizando el riesgo de error. Abstract: Background and objective: Chronic kidney disease (CKD) requires patients to participate in an adaptation process, which may be facilitated with the support of healthcare professionals and trained peers. The objective of this study is to present the implementation of a pilot patient mentoring programme to promote adaptation in patients with CKD. Materials and method: Pre-test-post-test design (quantitative and qualitative). The study was carried out in six hospitals in Spain. The instruments used to measure impact were ad-hoc scales (10-point Likert scale response format) on satisfaction and skill acquisition, as well as the creation of focus groups with eight patient mentors and 10 healthcare professionals. The programme was split into four phases: 1. Design and validation of the manualised programme's content, and selection of patient mentors; 2. Mentor training, satisfaction with training and skills acquired by the mentors; 3. Implementation of mutual support groups and profile of those attending these mutual support groups; 4. Assessment and results of the Mentoring programme. Results: In total, 39 mentors were trained on group management skills, as well as how to provide emotional support. 22 support groups were held, with 121 participants (22% carers). The 65% of the patients were attending the CKD clinic. 65% of the participating patients considered making some form of lifestyle change after taking part in the programme. All the items assessing satisfaction and usefulness scored very highly, achieving 8.5 out of 10 or above. Conclusions: This is the first manualised mentoring programme in CKD to be undertaken simultaneously in six Spanish hospitals. The manualised and highly structured nature of the programme make it easy to replicate, minimising the risk of error. Palabras clave: Adaptación a la enfermedad, Enfermedad renal crónica, Mentoring, Apoyo emocional entre pares, Keywords: Adaptation to the disease, Chronic kidney disease, Mentoring, Peer emotional support

Esta investigación presenta un análisis de las sedes web de 20 universidades cubanas adscritas al Ministerio de Educación Superior (MES) de Cuba. Su diseño está basado en la construcción de la representación analítica de cuatro dimensiones fundamentales: contenido, apariencia, accesibilidad y posicionamiento SEO. El objetivo esencial del trabajo es analizar el estado de los sitios webs de las universidades cubanas. Para ello, se procede a la definición y aplicación de un grupo de indicadores que nos permitirá analizar los contenidos, la apariencia, la accesibilidad y posicionamiento SEO respectivamente. Con base en la literatura científica sobre gestión de la comunicación digital y, de manera específica, sobre la gestión de sedes webs universitarias, se definieron esos indicadores y una serie de parámetros que permitieron evaluar la situación actual de la universidad cubana. Este estudio en las sedes web universitarias cubanas evidencia resultados no muy alentadores en la gestión de contenidos y, sobre todo, deficiencias sobre la comunicación y difusión de las funciones de estos centros, como instituciones que vinculan la docencia e investigación con el desarrollo social.

Introducción: debido a la emergencia del coronavirus SARS-CoV-2 desde diciembre del 2019, se ha generado un gran volumen de producción científica, en algún caso incierta o controvertida especialmente en el manejo farmacológico de los pacientes con esta infección; por lo tanto, se considera relevante buscar alternativas metodológicas para realizar su síntesis rigurosa, sistemática y de calidad, pero con menor tiempo de ejecución y menor costo. Objetivo: presentar la evidencia disponible respecto al manejo farmacológico de personas con sospecha o diagnóstico de infección respiratoria por SARS-CoV-2 (COVID-19) utilizando el método de revisiones sistemáticas rápidas (RS-R) en medicamentos poten- cialmente eficaces para su manejo. Metodología: se realizó una búsqueda sistemática y estructurada en Medline, Embase, Scopus, Cochrane Library, Clinical trials y Google Scholar en inglés. Los estudios incluidos fueron guías de práctica clínica, consensos, revisiones sistemáticas, metaanálisis, ensayos clínicos y otros estudios primarios. La búsqueda y extracción de datos se realizó por múltiples revisores, pero ninguna fue pareada. Resultados: dieciseis preguntas de interés clínico fueron resueltas, relacionadas con el uso en COVID-19 de lopinavir/ ritonavir, nelfinavir, oseltamivir, remdesivir, ribavirina, teicoplanina, umifenovir, favipiravir, tocilizumab, ivermectina y plasma convaleciente; también se evaluó el uso de medicamentos de soporte e incluidos en el manejo como la dexametasona, así como el uso concomitante de medicamentos que generaron dudas como son los AINES, los IECA y los ARA II. Conclusiones: los resúmenes de evidencia se muestran dentro del escenario de la pandemia como una buena alternativa metodológica para ofrecer información de calidad a corto plazo para los tomadores de decisiones.

En Colombia, actualmente no existen parámetros claros para el diagnóstico de pacientes con talla baja, ni sobre el tratamiento de esta población con hormona de crecimiento recombinante humana (somatropina), lo cual se ve favorecido por la diversidad de programas de formación de profesionales en endocrinología pediátrica. En respuesta a esta problemática se realizó el primer acuerdo colombiano de expertos en talla baja liderado por la Asociación Colegio Colombiana de Endocrinología Pediátrica (ACCEP); este trabajo contó con la participación y el aval de expertos clínicos de importantes instituciones de salud públicas y privadas del país, además de expertos metodológicos del instituto Keralty, quienes garantizaron la estandarización del uso de la somatropina. Después de realizar una minuciosa revisión de la literatura, se propone la unificación de definiciones, un algoritmo diagnóstico, los parámetros de referencia de las pruebas bioquímicas y dinámicas, una descripción de las consideraciones de uso de la somatropina para el tratamiento de las patologías con aprobación por la entidad regulatoria de medicamentos y alimentos en Colombia y, por último, un formato de consentimiento informado y de ficha técnica del medicamento.