1. On-chip flow cytometer using integrated photonics for the detection of human leukocytes
- Author
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Stijn Jooken, Kirill Zinoviev, Günay Yurtsever, Anabel De Proft, Koen de Wijs, Zeinab Jafari, Ana Lebanov, Gaudhaman Jeevanandam, Mateusz Kotyrba, Erwin Gorjup, Jelle Fondu, Liesbet Lagae, Sarah Libbrecht, Pol Van Dorpe, and Niels Verellen
- Subjects
Medicine ,Science - Abstract
Abstract Differentiation between leukocyte subtypes like monocytes and lymphocytes is essential for cell therapy and research applications. To guarantee the cost-effective delivery of functional cells in cell therapies, billions of cells must be processed in a limited time. Yet, the sorting rates of commercial cell sorters are not high enough to reach the required yield. Process parallelization by using multiple instruments increases variability and production cost. A compact solution with higher throughput can be provided by multichannel flow cytometers combining fluidics and optics on-chip. In this work, we present a micro-flow cytometer with monolithically integrated photonics and fluidics and demonstrate that both the illumination of cells, as well as the collection of scattered light, can be realized using photonic integrated circuits. Our device is the first with sufficient resolution for the discrimination of lymphocytes and monocytes. Innovations in microfabrication have enabled complete integration of miniaturized photonic components and fluidics in a CMOS-compatible wafer stack. In combination with external optics, the device is ready for the collection of fluorescence using the on-chip excitation.
- Published
- 2024
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