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In 2018 we celebrated 25 years of development of radar altimetry, and the progress achieved by this methodology in the fields of global and coastal oceanography, hydrology, geodesy and cryospheric sciences. Many symbolic major events have celebrated these developments, e.g., in Venice, Italy, the 15th (2006) and 20th (2012) years of progress and more recently, in 2018, in Ponta Delgada, Portugal, 25 Years of Progress in Radar Altimetry. On this latter occasion it was decided to collect contributions of scientists, engineers and managers involved in the worldwide altimetry community to depict the state of altimetry and propose recommendations for the altimetry of the future. This paper summarizes contributions and recommendations that were collected and provides guidance for future mission design, research activities, and sustainable operational radar altimetry data exploitation. Recommendations provided are fundamental for optimizing further scientific and operational advances of oceanographic observations by altimetry, including requirements for spatial and temporal resolution of altimetric measurements, their accuracy and continuity. There are also new challenges and new openings mentioned in the paper that are particularly crucial for observations at higher latitudes, for coastal oceanography, for cryospheric studies and for hydrology. The paper starts with a general introduction followed by a section on Earth System Science including Ocean Dynamics, Sea Level, the Coastal Ocean, Hydrology, the Cryosphere and Polar Oceans and the “Green” Ocean, extending the frontier from biogeochemistry to marine ecology. Applications are described in a subsequent section, which covers Operational Oceanography, Weather, Hurricane Wave and Wind Forecasting, Climate projection. Instruments’ development and satellite missions’ evolutions are described in a fourth section. A fifth section covers the key observations that altimeters provide and their potential complements, from other Earth observation measurements to in situ data. Section 6 identifies the data and methods and provides some accuracy and resolution requirements for the wet tropospheric correction, the orbit and other geodetic requirements, the Mean Sea Surface, Geoid and Mean Dynamic Topography, Calibration and Validation, data accuracy, data access and handling (including the DUACS system). Section 7 brings a transversal view on scales, integration, artificial intelligence, and capacity building (education and training). Section 8 reviews the programmatic issues followed by a conclusion.

Background: Spatial acuity concerns the ability to localize and discriminate sensory input and is often tested using the two‐point discrimination threshold (2PDT). Sensitization of the pain system can affect the spatial acuity, but it is unclear how 2PDTs of different testing modalities are affected. The aim was to investigate if the 2PDTs for mechanical and heat stimulation at different intensities were modulated by topical capsaicin sensitization. Methods: 30 healthy subjects were divided into either a capsaicin or a placebo group. The 2PDT was tested using two different modalities, mechanical and thermal (laser) delivered at innocuous and noxious intensities. The 2PDT were determined at baseline and re‐assessed 48 h later. In the follow‐up session, the subjects either had a capsaicin patch (8%) or placebo patch placed in the testing area for 30 min before re‐testing the 2PDT. Results: The 2PDT was highly dependent on stimulation modality and intensity. The lowest 2PDT was found for innocuous mechanical stimuli (40.0 mm, 95% CI 38.1–41.9 mm), and the highest 2PDT was found for innocuous thermal stimuli (81.7 mm, 95% CI 73.9–89.5 mm). Topical capsaicin generally increased the 2PDT, but this was only significant for innocuous mechanical stimuli. The perceived intensity of the stimuli was increased following capsaicin and was generally higher for noxious stimuli than for innocuous stimuli (ANOVA, p < 0.001). Conclusions: This study showed that capsaicin provoked pain sensitization increased the 2PDT. The 2PDT tested using innocuous mechanical stimuli showed less variable results indicating that this test is most suitable to detect this aspect of spatial acuity. Significance Statement: This study investigated how the two‐point discrimination threshold (2PDT) can be modulated by topical capsaicin. The 2PDT was assessed for two different modalities (thermal and mechanical) and for two different intensities (innocuous and noxious) before and after capsaicin. The results showed that the 2PDT was generally impaired following capsaicin, but this was only significant for mechanical innocuous stimuli. Furthermore, it was shown that mechanical innocuous stimuli assessed the 2PDT with lower variability than other combinations. [ABSTRACT FROM AUTHOR]

Background: Commonly used models for eliciting muscle pain involve the injection of algesic substances or the induction of delayed onset muscle soreness. The former require invasive procedures, and the time frame for pain induction and subsidence in the latter can be inconvenient. This study presents a detailed spatiotemporal characterization of a new experimental model of muscle pain based on short‐wave diathermy (SWD), developed to overcome the limitations of existing models. Methods: The shoulder was selected as target site and the effects of the model were tested in two sessions to assess its reliability. Pain intensity profiles were recorded during the application of SWD, and changes in pressure pain threshold (PPT) in the infraspinatus muscle, together with pain intensity, duration, and quality were assessed 30 min after induction. Results: SWD‐induced pain intensity scores averaged 4 points on a visual analogue scale, whereas PPT showed a consistent decrease of about 25% relative to baseline values. Pain was localized in the shoulder area, and was described as continuous, dull, well‐delimited, heavy, and bearable. Pain lasted for an average of 145 min without requiring reinduction and was reliably elicited in both experimental sessions. Conclusion: SWD can be used to elicit experimental muscle pain in a non‐invasive, long‐lasting, and reliable way and allows for repeated within‐ and between‐session testing in the shoulder. Significance Statement: SWD produces deep heating in muscles by converting electromagnetic energy to thermal energy. It was previously shown that it can be used to elicit experimental pain in the forearm muscles, and the present study demonstrates that this can be reliably generalized to other body sites, such as the shoulder. Furthermore, SWD application is non‐invasive and presents a convenient time frame for pain induction and subsidence, thus overcoming limitations associated with traditional muscle pain models. [ABSTRACT FROM AUTHOR]

Accurate shallow-water depth information for island areas is crucial for maritime safety, resource exploration, ecological conservation, and offshore economic activity. Traditional approaches like shipborne sounding and airborne bathymetric light detection and ranging (LiDAR) surveys are expensive, time-consuming, and are limited in politically sensitive regions. Moreover, satellite altimetry-predicted depths exhibit large errors over shallow waters. In contrast, satellite-derived bathymetry (SDB), estimated from multispectral imagery, provides a rapid, open source, and cost-effective technique to fully characterize the bathymetry of a region. Given the scarcity of in-situ water-depth data for the South China Sea (SCS), a shallow-water depth model, HHU24SWDSCS, was developed by integrating 1298 Ice, Cloud, and land Elevation Satellite (ICESat-2) tracks with 70 Sentinel-2 multispectral images. The model covers >120 islands and reefs in the SCS, with a resolution of 10 m. Validation against independent ICESat-2 depth data produced a root mean square error for the model of 0.81–1.35 m (<5 % of the maximum depth), with an average coefficient of determination of 0.91. Validation against independent airborne LiDAR bathymetry data revealed an accuracy of 1.01 m for the Lingyang Reef. Further comparisons with existing bathymetry models revealed the superior performance of the model. While the existing bathymetry models exhibit errors up to tens of meters or larger for island regions, and should therefore be used with caution, the HHU24SWDSCS model exhibited good accuracy in shallow waters across the SCS. This model thus provides a reference for mapping shallow-water depth close to islands and provides fundamental support for research in oceanography, geodesy, and other disciplines. [ABSTRACT FROM AUTHOR]

Carbohydrates are critical for high‐intensity exercise performance. However, the effects of carbohydrate supplementation on muscle metabolism and performance during short‐duration high‐intensity intermittent exercise remain inadequately explored. Our aim was to address this aspect in a randomized, counterbalanced, double‐blinded crossover design. Eleven moderately‐to‐well‐trained males performed high‐intensity intermittent cycling receiving carbohydrate (CHO, ~55 g/h) or placebo (PLA) fluid supplementation. Three exercise periods (EX1‐EX3) were completed comprising 10 × 45 s at ~105% Wmax interspersed with 135 s rest between bouts and ~20 min between periods. Repeated sprint ability (5 × 6 s sprints with 24 s recovery) was assessed at baseline and after each period. Thigh muscle biopsies were obtained at baseline and before and after EX3 to determine whole‐muscle and fiber‐type‐specific glycogen depletion. No differences were found in muscle glycogen degradation at the whole‐muscle (p = 0.683) or fiber‐type‐specific level (p = 0.763–0.854) with similar post‐exercise whole‐muscle glycogen concentrations (146 ± 20 and 122 ± 15 mmol·kg−1 dw in CHO and PLA, respectively). Repeated sprint ability declined by ~9% after EX3 with no between‐condition differences (p = 0.971) and no overall differences in ratings of perceived exertion (p = 0.550). This was despite distinctions in blood glucose concentrations throughout exercise, reaching post‐exercise levels of 5.3 ± 0.2 and 4.1 ± 0.2 mmol·L−1 (p < 0.001) in CHO and PLA, respectively, accompanied by fivefold higher plasma insulin levels in CHO (p < 0.001). In conclusion, we observed no effects of carbohydrate ingestion on net muscle glycogen breakdown or sprint performance during short‐duration high‐intensity intermittent exercise despite elevated blood glucose and insulin levels. These results therefore question the efficacy of carbohydrate supplementation strategies in high‐intensity intermittent sports. [ABSTRACT FROM AUTHOR]

Background: Cutaneous laser stimulation has commonly been employed to investigate the thermal properties of the nociceptive system. The aim of this study was to investigate how a temperature‐controlled laser system improves the assessment of directional discrimination in the nociceptive system. Methods: In total, twenty healthy volunteers participated in this study. To determine the directional discrimination threshold (stimulation length 50% correct, expressed in mm), thermal stimuli were delivered using a diode laser and the laser beam was perpendicularly displaced across the skin to give a linear stimulation in four different directions (distal, proximal, lateral and medial) and displacement lengths (3 for lateral‐medial and 5 for distal‐proximal). Two temperature control modes were used in the stimulation system, open‐loop and closed‐loop control. The subjects had to report the perceived stimulus direction, the degree of certainty regarding the perceived direction and the intensity of the perceived stimulus (0–10 numerical rating scale, 3: pain threshold). Results: During closed‐loop control, the orientation of stimuli was discriminated significantly more accurately than during open‐loop control. During closed‐loop control, the directional discrimination threshold was 31.9 and 26.1 mm for distal‐proximal and lateral‐medial directed stimuli, respectively. A numerical rating scale was significantly higher for the lateral/medial directions. Moreover, the variability of the discrimination threshold is reduced in the closed‐loop control system. Conclusions: The findings show that discrimination ability is better in the lateral‐medial directions compared to the distal‐proximal directions. This study indicates that using a system enabling closed‐loop temperature control, allows more robust probing of the temporo‐spatial mechanisms in the nociceptive system. Significance: This study shows that a newly developed temperature‐controlled laser stimulation system enhances the possibilities to investigate the nociceptive temporo‐spatial integration, as shown by a less variable directional discrimination threshold. The results also show that different orthogonal directions are discriminated differently. This new method allows a better investigation of the combined temporal and spatial mechanisms in the nociceptive system. [ABSTRACT FROM AUTHOR]

Southwest Greenland constitutes an internationally important wintering area for seabirds, including Thick-billed Murres (Uria lomvia (Linnaeus, 1758)), but their prey may be affected by the general warming of this sub-Arctic region. We compared murre diet collected in winter in the 1990s and in the 2010s around Nuuk, Greenland. Fish made up 36% of the diet (wet mass) and crustaceans 63% in the 1990s, changing to 22% and 78% in the 2010s, respectively. Capelin (Mallotus villosus (Muller, 1776)) was the dominant fish species, and the smaller contribution in the 2010s coincided with declining densities of capelin around Nuuk. The crustaceans were dominated by two krill species (Meganyctiphanes norvegica (M. Sars, 1857) and Thysanoessa inermis (Kroyer, 1846)). However, M. norvegica was only important in the 2010s (51% wet mass), while T. inermis was dominating the 1990s with 62% wet mass and only 23% in the 2010s. The dominance of M. norvegica in the 2010s confirmed our expectations of a gradual 'borealization' of this region due to the generally warming sub-Arctic. The smaller contribution of fish in the diet may also support the hypothesis of deteriorating winter conditions for murres. Apart from the diet, plastic was found in 15% of the birds and 53% had parasitic nematodes. Key words: seabird winter diet, Thick-billed Murre, Uria lomvia, southwest Greenland, borealization, krill, capelin, Mallotus villosus. Si le sud-ouest du Groenland constitue une aire d'hivernage d'importance internationale pour les oiseaux marins, incluant le guillemot de Brunnich (Uria lomvia (Linnaeus, 1758)), le rechauffement general de cette region subarctique pourrait avoir une incidence sur leurs proies. Nous comparons les regimes alimentaires hivernaux de guillemots durant les annees 1990 et 2010 dans les environs de Nuuk (Groenland). Les poissons constituaient 36 % (masse humide) de leur alimentation et les crustaces, 63 %, dans les annees 1990, ces proportions passant a 22 % et 78 %, respectivement, durant les annees 2010. Le capelan (Mallotus villosus (Muller, 1776)) etait l'espece de poisson dominante, et le plus faible pourcentage de poissons dans les annees 2010 coincide avec des densites de capelans reduites aux environs de Nuuk. Parmi les crustaces consommes, deux especes de krill (Meganyctiphanes norvegica (M. Sars, 1857) et Thysanoessa inermis (Kroyer, 1846)) dominaient. Toutefois, M. norvegica n'etait important que dans les annees 2010 (51 % masse humide), alors que T. inermis etait dominant dans les annees 1990 (62 % masse humide, contre seulement 23 % durant les annees 2010). La predominance de M. norvegica dans les annees 2010 confirme nos predictions d'une <> progressive de cette region decoulant du rechauffement general de la region subarctique. La diminution de la proportion des poissons dans l'alimentation pourrait egalement appuyer l'hypothese de la deterioration des conditions hivernales pour les guillemots. Du plastique a egalement ete retrouve dans 15 % des oiseaux, et 53 % etaient parasites par des nematodes. [Traduit par la Redaction] Mots-cles: alimentation hivernale des oiseaux marins, guillemot de Brunnich, Uria lomvia, sud-ouest du Groenland, borealisation, krill, capelan, Mallotus villosus., Introduction For migratory bird populations, the non-breeding season represents a very long and usually challenging period of their annual cycle, and their survival and subsequent breeding performance are highly dependent [...]

The coastal marine gravity field is not well modelled due to poor data coverage. Recent satellite altimeters provide reliable altimetry observations near the coast, filling the gaps between the open ocean and land. We show the potential of recent satellite altimetry for the coastal marine gravity modelling using the least squares collocation technique. Gravity prediction error near the coast is better than 4 mGal. The modelled gravity anomalies are validated with sparse shipborne gravimetric measurements. We obtained 4.86 mGal precision when using the altimetry data with the best coastal coverage and retracked with narrow primary peak retracker. The predicted gravity field is an enhancement to EGM2008 over the coastal regions. The potential improvement in alti- metric marine gravity will be beneficial for the next generation of EGM development.

Objectives: To explore the antimicrobial potential of strontium (Sr)‐functionalized wafers against multiple bacteria associated with per‐implant infections, in both mono‐ and multispecies biofilms. Materials and Methods: The bactericidal and bacteriostatic effect of silicon wafers functionalized with a strontium titanium oxygen coating (Sr‐Ti‐O) or covered only with Ti (controls) against several bacteria, either grown as a mono‐species or multispecies biofilms, was assessed using a bacterial viability assay and a plate counting method. Mono‐species biofilms were assessed after 2 and 24 h, while the antimicrobial effect on multispecies biofilms was assessed at Days 1, 3, and 6. The impact of Sr functionalization on the total percentage of Porphyromonas gingivalis in the multispecies biofilm, using qPCR, and gingipain activity was also assessed. Results: Sr‐functionalized wafers, compared to controls, were associated with statistically significant less viable cells in both mono‐ and multispecies tests. The number of colony forming units (CFUs) within the biofilm was significantly less in Sr‐functionalized wafers, compared to control wafers, for Staphylococcus aureus at all time points of evaluation and for Escherichia coli at Day 1. Gingipain activity was less in Sr‐functionalized wafers, compared to control wafers, and the qPCR showed that P. gingivalis remained below detection levels at Sr‐functionalized wafers, while it consisted of 15% of the total biofilm on control wafers at Day 6. Conclusion: Sr functionalization displayed promising antimicrobial potential, possessing bactericidal and bacteriostatic ability against bacteria associated with peri‐implantitis grown either as mono‐species or mixed in a multispecies consortium with several common oral microorganisms. [ABSTRACT FROM AUTHOR]