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Abstract There is no reliable automated non-invasive solution for monitoring circulation and guiding treatment in prehospital emergency medicine. Cardiac output (CO) monitoring might provide a solution, but CO monitors are not feasible/practical in the prehospital setting. Non-invasive ballistocardiography (BCG) measures heart contractility and tracks CO changes. This study analyzed the feasibility of estimating CO using morphological features extracted from BCG signals. In 20 healthy subjects ECG, carotid/abdominal BCG, and invasive arterial blood pressure based CO were recorded. BCG signals were adaptively processed to isolate the circulatory component from carotid (CCc) and abdominal (CCa) BCG. Then, 66 features were computed on a beat-to-beat basis to characterize amplitude/duration/area/length of the fluctuation in CCc and CCa. Subjects’ data were split into development set (75%) to select the best feature subset with which to build a machine learning model to estimate CO and validation set (25%) to evaluate model’s performance. The model showed a mean absolute error, percentage error and 95% limits of agreement of 0.83 L/min, 30.2% and − 2.18–1.89 L/min respectively in the validation set. BCG showed potential to reliably estimate/track CO. This method is a promising first step towards an automated, non-invasive and reliable CO estimator that may be tested in prehospital emergencies.

This study explores the potential of training robots using reinforcement learning (RL) to adapt their behavior based on human comfort levels during tasks. An experimental environment has been developed and made available to the research community, facilitating the replication of these experiments. The results demonstrate that adjusting a single comfort-related input parameter during training leads to significant variations in the robot’s behavior. Detailed discussions of the reward functions and obtained results validate these behavioral adaptations, confirming that robots can dynamically respond to human needs, thereby enhancing human-robot interaction. While the study highlights the effectiveness of this approach, it also raises the question of real-time comfort measurement, suggesting various systems for future exploration. These findings contribute to the development of more intuitive and emotionally responsive robots, offering new possibilities for future research in advancing human-robot interaction.

The objectives of this study were to compare the effects of two in-season velocity loss training methods (VBT) on performance outcomes and to evaluate the effects of velocity-based training compared to traditional resistance training (TRT) on performance outcomes in young elite soccer players. VBT utilized the same relative load but varied in the extent of velocity loss during the set: 15% (VL15%) and 30% (VL30%). Thirty-four players were recruited and randomly distributed into three groups: the VL15% group (n = 12; age = 18.50 ± 0.67 years; stature = 183.41 ± 4.25 cm; body mass = 75.08 ± 5.57 kg), the VL30% group (n = 11; age = 17.91 ± 0.60 years; stature = 181.21 ± 6.56 cm, body mass = 73.58 ± 6.22 kg), and the traditional strength training group TRT (n = 11; age = 18.14 ± 0.74 years; stature = 182.17 ± 5.52 cm; body mass = 74.86 ± 6.68 kg). Alongside regular soccer sessions and matches, the groups underwent a four-week (2 sesions per week) resistance training intervention with back squats involved. Changes in leg strength (SQ1RM), 20 m sprint time (SPR 20 m), countermovement jump height (CMJ), reactive strength index (RSI), and change of direction (COD) from before and after were evaluated using a 3 × 2 ANOVA. While no significant interaction was found for SQ1RM and SPR20, all of the groups showed significant pre to post improvements. Significant interactions were observed for CMJ (F = 38.24, p = 0.000), RSI (F = 8.33; p = 0.001), and change of direction agility test (COD) (F = 3.64; p = 0.038), with a post hoc analysis showing differences between the VL15 (6.0%) and TRT (1.7%) groups (p = 0.034); VL15 (12.2%) and VL30 (3.2%) groups (p = 0.004); VL15 and TRT (0.4%) (p = 0.018); VL15 (2.4%) and VL30 (1.5%) (p = 0.049); and between the VL15 and TRT (0.4%) groups (p = 0.015). Four weeks of VL15% training during the season induced similar strength increases to VL30% and TRT, superior improvements in RSI and COD compared to VL30%, and superior improvements in CMJ, RSI, and COD tests compared to TRT. Thus, incorporating the VL15% training method may be recommended to improve power-related performance metrics in elite young soccer players.

Collaborative robotics is a major topic in current robotics research, posing new challenges, especially in human–robot interaction. The main aspect in this area of research focuses on understanding the behavior of robots when engaging with humans, where reinforcement learning is a key discipline that allows us to explore sophisticated emerging reactions. This review aims to delve into the relevance of different sensors and techniques, with special attention to EEG (electroencephalography data on brain activity) and its influence on the behavior of robots interacting with humans. In addition, mechanisms available to mitigate potential risks during the experimentation process such as virtual reality are also be addressed. In the final part of the paper, future lines of research combining the areas of collaborative robotics, reinforcement learning, virtual reality, and human factors are explored, as this last aspect is vital to ensuring safe and effective human–robot interactions.

Background: During pulseless electrical activity (PEA) the cardiac mechanical and electrical functions are dissociated, a phenomenon occurring in 25–42% of in-hospital cardiac arrest (IHCA) cases. Accurate evaluation of the likelihood of a PEA patient transitioning to return of spontaneous circulation (ROSC) may be vital for the successful resuscitation. The aim: We sought to develop a model to automatically discriminate between PEA rhythms with favorable and unfavorable evolution to ROSC. Methods: A dataset of 190 patients, 120 with ROSC, were acquired with defibrillators from different vendors in three hospitals. The ECG and the transthoracic impedance (TTI) signal were processed to compute 16 waveform features. Logistic regression models where designed integrating both automated features and characteristics annotated in the QRS to identify PEAs with better prognosis leading to ROSC. Cross validation techniques were applied, both patient-specific and stratified, to evaluate the performance of the algorithm. Results: The best model consisted in a three feature algorithm that exhibited median (interquartile range) Area Under the Curve/Balanced accuracy/Sensitivity/Specificity of 80.3(9.9)/75.6(8.0)/ 77.4(15.2)/72.3(16.4) %, respectively. Conclusions: Information hidden in the waveforms of the ECG and TTI signals, along with QRS complex features, can predict the progression of PEA. Automated methods as the one proposed in this study, could contribute to assist in the targeted treatment of PEA in IHCA.

Aims: We designed a two-part epidemiological study, an observatory for amyloid transthyretin amyloidosis (OBSAMYL). The first objective was to identify and count the number of patients diagnosed with ATTR amyloidosis in participating French centres. The second was to evaluate the use and safety profile of tafamidis meglumine in real-world settings. Methods: This was a non-interventional descriptive retrospective multi-centre national study. A census was conducted to estimate the number of patients diagnosed with ATTR amyloidosis who were still alive at the time of the study (defined as 1 June 2017). Patients with ATTR amyloidosis were contacted by French centres from the French Rare Diseases network program. Patients aged ≥18 years with hereditary transthyretin-mediated amyloidosis (ATTRv) or wild-type transthyretin amyloidosis (ATTRwt) or a pathogenic transthyretin (TTR) mutation were eligible. Results: Of the 38 centres (13 cardiology and 25 neurology) invited to participate, 22 (60.5%) (10 cardiology, 12 neurology) participated. There were 333 patients in cardiology census population. Before diagnosis one-fourth of the patients had cardiac decompensation, and one-fifth had a pacemaker. The 177 ATTRwt-CM patients were older (80.1 ± 7.0 years versus 64.2 ± 14.3 years; P < 0.001), had a higher incidence of hypertension (51.4% versus 35.3%; P = 0.003), and a higher incidence of arrhythmia (45.8% versus 28.3%; P = 0.001) than 156 ATTRv patients. There were no differences in disease severity according to New York Heart Association classification. The ATTRv-mixed + CM group had more neurological symptoms (paraesthesia or dysesthesia, neuropathic pain, digestive disorders, and orthostatic hypotension) than the ATTRwt-CM group (P < 0.001). Biopsies were performed on nearly 90% of patients with most of them being positive. The most common biopsy sites were salivary glands (137 biopsies) and cardiac tissues (77 biopsies). Tafamidis meglumine was administered to 174 cardiology patients, including 96 with ATTRv-mixed, 61 with ATTRwt-CM, and 17 with ATTRv-CM. Tafamidis meglumine was generally well tolerated. 18 adverse events, including 12 severe adverse events were reported in 174 patients as safety-related incidents. Tafamidis meglumine was likely responsible for five adverse events, one of which was severe. Conclusion: This study of real-world clinical ATTR amyloidosis cases in France further elucidated the characteristics of and diagnostic approach to a cardiology patient population census of 333 patients. As of June 1, 2017, 177 ATTRwt-CM, 117 ATTRv-mixed, and 39 ATTRv-CM patients were alive. Our experience with tafamidis meglumine in the cardiology population confirmed its good tolerance.

Cardiac auscultation is an accessible diagnostic screening tool that can help to identify patients with heart murmurs, who may need follow-up diagnostic screening and treatment for abnormal cardiac function. However, experts are needed to interpret the heart sounds, limiting the accessibility of cardiac auscultation in resource-constrained environments. Therefore, the George B. Moody PhysioNet Challenge 2022 invited teams to develop algorithmic approaches for detecting heart murmurs and abnormal cardiac function from phonocardiogram (PCG) recordings of heart sounds. For the Challenge, we sourced 5272 PCG recordings from 1452 primarily pediatric patients in rural Brazil, and we invited teams to implement diagnostic screening algorithms for detecting heart murmurs and abnormal cardiac function from the recordings. We required the participants to submit the complete training and inference code for their algorithms, improving the transparency, reproducibility, and utility of their work. We also devised an evaluation metric that considered the costs of screening, diagnosis, misdiagnosis, and treatment, allowing us to investigate the benefits of algorithmic diagnostic screening and facilitate the development of more clinically relevant algorithms. We received 779 algorithms from 87 teams during the Challenge, resulting in 53 working codebases for detecting heart murmurs and abnormal cardiac function from PCG recordings. These algorithms represent a diversity of approaches from both academia and industry, including methods that use more traditional machine learning techniques with engineered clinical and statistical features as well as methods that rely primarily on deep learning models to discover informative features. The use of heart sound recordings for identifying heart murmurs and abnormal cardiac function allowed us to explore the potential of algorithmic approaches for providing more accessible diagnostic screening in resource-constrained environments. The submission of working, open-source algorithms and the use of novel evaluation metrics supported the reproducibility, generalizability, and clinical relevance of the research from the Challenge.

Abstract Background Facioscapulohumeral muscular dystrophy (FSHD) is among the most prevalent muscular dystrophies and currently has no treatment. Clinical and genetic heterogeneity are the main challenges to a full comprehension of the physiopathological mechanism. Improving our knowledge of FSHD is crucial to the development of future therapeutic trials and standards of care. National FSHD registries have been set up to this end. The French National Registry of FSHD combines a clinical evaluation form (CEF) and a self-report questionnaire (SRQ), filled out by a physician with expertise in neuromuscular dystrophies and by the patient, respectively. Aside from favoring recruitment, our strategy was devised to improve data quality. Indeed, the pairwise comparison of data from 281 patients for 39 items allowed for evaluating data accuracy. Kappa or intra-class coefficient (ICC) values were calculated to determine the correlation between answers provided in both the CEF and SRQ. Results Patients and physicians agreed on a majority of questions common to the SRQ and CEF (24 out of 39). Demographic, diagnosis- and care-related questions were generally answered consistently by the patient and the medical practitioner (kappa or ICC values of most items in these groups were greater than 0.8). Muscle function-related items, i.e. FSHD-specific signs, showed an overall medium to poor correlation between data provided in the two forms; the distribution of agreements in this section was markedly spread out and ranged from poor to good. In particular, there was very little agreement regarding the assessment of facial motricity and the presence of a winged scapula. However, patients and physicians agreed very well on the Vignos and Brooke scores. The report of symptoms not specific to FSHD showed general poor consistency. Conclusions Patient and physician answers are largely concordant when addressing quantitative and objective items. Consequently, we updated collection forms by relying more on patient-reported data where appropriate. We hope the revised forms will reduce data collection time while ensuring the same quality standard. With the advent of artificial intelligence and automated decision-making, high-quality and reliable data are critical to develop top-performing algorithms to improve diagnosis, care, and evaluate the efficiency of upcoming treatments.

This study presents a model for predicting photovoltaic power generation based on meteorological, temporal and geographical variables, without using irradiance values, which have traditionally posed challenges and difficulties for accurate predictions. Validation methods and evaluation metrics are used to analyse four different approaches that vary in the distribution of the training and test database, and whether or not location-independent modelling is performed. The coefficient of determination, R2, is used to measure the proportion of variation in photovoltaic power generation that can be explained by the model’s variables, while gCO2eq represents the amount of CO2 emissions equivalent to each unit of power generation. Both are used to compare model performance and environmental impact. The results show significant differences between the locations, with substantial improvements in some cases, while in others improvements are limited. The importance of customising the predictive model for each specific location is emphasised. Furthermore, it is concluded that environmental impact studies in model production are an additional step towards the creation of more sustainable and efficient models. Likewise, this research considers both the accuracy of solar energy predictions and the environmental impact of the computational resources used in the process, thereby promoting the responsible and sustainable progress of data science.

Boutary et al. describe siRNA based therapy conjugated with squalene nanoparticles as an efficient approach to normalize PMP22 protein levels, restore locomotor activity, electrophysiological parameters and function of myelin sheath in CMT1A mouse models. These findings could be useful to develop therapeutic strategies for inherited peripheral neuropathies.

Modern photonic technologies are emerging, allowing the acquisition of in-vivo endoscopic tissue imaging at a microscopic scale, with characteristics comparable to traditional histological slides, and with a label-free modality. This raises the possibility of an `optical biopsy' to aid clinical decision making. This approach faces barriers for being incorporated into clinical practice, including the lack of existing images for training, unfamiliarity of clinicians with the novel image domains and the uncertainty of trusting `black-box' machine learned image analysis, where the decision making remains inscrutable. In this paper, we propose a new method to transform images from novel photonics techniques (e.g. autofluorescence microscopy) into already established domains such as Hematoxilyn-Eosin (H-E) microscopy through virtual reconstruction and staining. We introduce three main innovations: 1) we propose a transformation method based on a Siamese structure that simultaneously learns the direct and inverse transformation ensuring domain back-transformation quality of the transformed data. 2) We also introduced an embedding loss term that ensures similarity not only at pixel level, but also at the image embedding description level. This drastically reduces the perception distortion trade-off problem existing in common domain transfer based on generative adversarial networks. These virtually stained images can serve as reference standard images for comparison with the already known H-E images. 3) We also incorporate an uncertainty margin concept that allows the network to measure its own confidence, and demonstrate that these reconstructed and virtually stained images can be used on previously-studied classification models of H-E images that have been computationally degraded and de-stained. The three proposed methods can be seamlessly incorporated on any existing architectures. We obtained balanced accuracies of 0.95 and negative predictive values of 1.00 over the reconstructed and virtually stained image-set on the detection of color-rectal tumoral tissue. This is of great importance as we reduce the need for extensive labeled datasets for training, which are normally not available on the early studies of a new imaging technology.

Abstract Recessive mutations in PYROXD1, encoding an oxidoreductase, were recently reported in families with congenital myopathy or limb-girdle muscular dystrophy. Here we describe three novel PYROXD1 families at the clinical, histological, and genetic level. Histological analyses on muscle biopsies from all families revealed fiber size variability, endomysial fibrosis, and muscle fibers with multiple internal nuclei and cores. Further characterization of the structural muscle defects uncovered aggregations of myofibrillar proteins, and provided evidence for enhanced oxidative stress. Sequencing identified homozygous or compound heterozygous PYROXD1 mutations including the first deep intronic mutation reinforcing a cryptic donor splice site and resulting in mRNA instability through exonisation of an intronic segment. Overall, this work expands the PYROXD1 mutation spectrum, defines and specifies the histopathological hallmarks of the disorder, and indicates that oxidative stress contributes to the pathomechanism. Comparison of all new and published cases uncovered a genotype/phenotype correlation with a more severe and early-onset phenotypic presentation of patients harboring splice mutations resulting in reduced PYROXD1 protein levels compared with patients carrying missense mutations.

The CoronaVac vaccine is the most used anti-SARS-CoV-2 vaccine worldwide. Previous data indicate that this vaccine produces a lower immune response than RNA vaccines such as BNT162b2. End-stage renal disease (ESRD) patients have an increased rate of COVID-19 and a reduced immune response to vaccinations. Currently, there is little data on this population’s immune response induced by CoronaVac. Methods: This study involved a prospective cohort of ESRD patients in chronic hemodialysis who received a two-dose immunization scheme of either CoronaVac (Sinovac Biotech) or BNT162b2 vaccines (Pfizer-BioNTech). We measured the plasma levels of anti-SARS-CoV-2 IgG antibodies. We determined antibody titers before immunization, 2 and 4 months after two doses, plus 4 months after a booster dose. Results: We evaluated 208 patients in three hemodialysis centers. The mean age was 62.6 ± 15.6 years, of whom 91 were female (41.75%). Eighty-one patients (38.94%) received the BNT162b2 vaccine and 127 (61.06%) received the CoronaVac vaccine. Patients who received the BNT162b2 vaccine had a higher humoral response compared to those who received the CoronaVac vaccine (4 months after the second dose: BNT162b2: 88.89%, CoronaVac: 51.97%, p < 0.001; 4 months after the booster: BNT162b2: 98.77%, CoronaVac: 86.61%, p < 0.001). Conclusions: Our results suggest that the CoronaVac vaccine induced a lower humoral response than the BNT162b2 vaccine in ESRD patients on hemodialysis.

At the center of the debate about the social impact of the university we find a new division of work in the production of knowledge that can respond to the challenges of society. This new division of work is more complex and signals an epistemological turn in the form of organizing interaction between academic knowledge and society. This article poses four challenges that were identified based on a case of the construction of a dialogic space for the co-generation of knowledge. The case, which was developed through action research, questions the scheme in which knowledge generated in a university is packaged in the form of articles, books, reports, or conferences and communicated linearly, and suggests another that is based on dialogue and that allows other actors to contribute their knowledge as well.

Nations and territories have new and varied challenges with respect to promoting continuous growth in the economy. The purpose of this article is to determine the opportunities and difficulties faced by the territory of Gipuzkoa in the field of economics. In interviews and discussion groups with the principle agents of the territory, the unified commitment to industry over the last decades is unanimously appreciated. This commitment involves the complete transformation of the social and economic structure of the territory. However, disparities in business dynamism are evident, and the capacities and resources of companies to bring industry up to date in the digital economy and respond to new competitive factors are very different. This being the case, different perspectives are emerging to respond to challenges among agents. Nevertheless, two general issues are agreed upon: the territory is conceptualized as an active agent, and the need for updating the innovation system is recognized. This makes it possible to share strategies and to respond in a cooperative way to productive specialization.

Corrosion is an important factor affecting considerably not only the time of use, but also the mechanical properties of metal objects in general. Moreover, corrosion causes economical loses and has an impact on the environment. This fenomenon is of greater importance for steel equipments and materials used in industry. In many industrial processes acid solutions are used which accelerate corrosion. In order to minimize this fenomenon different approaches have been taken, inhibitor use beeing one of them. This paper reports on the protection efficiency of an ionic liquid, 1-decyl-3-methylimidazolium chloride in the corrosion protection of 36CrMo steel in HCl solutions of different concentrations and temperatures. The corrosion velocity and protection effiecieny have been assessed using the gravimetric method. The results show that an increase in the 1-decyl-3-methylimidazolium chloride concentration results in lower corrosion velocity of the 36CrMo steel in acid environment. The protection effieciency decreased with the increase of temperature. The mechanism of corrosion protection for this inhibitor is also discussed in the paper. [ABSTRACT FROM AUTHOR]

La formulación de las políticas de investigación y desarrollo está siendo transformada de manera novedosa en los documentos más recientes que se elaboran en Europa. Una de las peculiaridades es que los patrones de investigación transitan hacia prácticas científico-tecnológicas más interactivas entre los distintos actores de la sociedad. Así, como respuesta a la naturaleza compleja de la investigación las primeras medidas se revelan en aquellas estrategias para la promoción de actitudes emprendedoras y colaborativas entre los actores académico-empresariales de la investigación y el desarrollo. A estas iniciativas se superponen otros planteamientos que trascienden el objetivo primariamente economicista de la colaboración e integran los aspectos sociales y éticos en la investigación y el desarrollo. Sin embargo, estas transformaciones también están sujetas a diversas tensiones de base y a objetivos concurrentes. El artículo dilucida estas dinámicas de apertura-y-cierre y manifiesta que algunas de las relaciones y demandas se muestran más resistentes al cambio.

Pulseless electrical activity (PEA) is characterized by the disassociation of the mechanical and electrical activity of the heart and appears as the initial rhythm in 20–30% of out-of-hospital cardiac arrest (OHCA) cases. Predicting whether a patient in PEA will convert to return of spontaneous circulation (ROSC) is important because different therapeutic strategies are needed depending on the type of PEA. The aim of this study was to develop a machine learning model to differentiate PEA with unfavorable (unPEA) and favorable (faPEA) evolution to ROSC. An OHCA dataset of 1921 5s PEA signal segments from defibrillator files was used, 703 faPEA segments from 107 patients with ROSC and 1218 unPEA segments from 153 patients with no ROSC. The solution consisted of a signal-processing stage of the ECG and the thoracic impedance (TI) and the extraction of the TI circulation component (ICC), which is associated with ventricular wall movement. Then, a set of 17 features was obtained from the ECG and ICC signals, and a random forest classifier was used to differentiate faPEA from unPEA. All models were trained and tested using patientwise and stratified 10-fold cross-validation partitions. The best model showed a median (interquartile range) area under the curve (AUC) of 85.7(9.8)% and a balance accuracy of 78.8(9.8)%, improving the previously available solutions at more than four points in the AUC and three points in balanced accuracy. It was demonstrated that the evolution of PEA can be predicted using the ECG and TI signals, opening the possibility of targeted PEA treatment in OHCA.

This paper aims to provide the historical and conceptual bases underlying the inclusionary transition of European innovation policy, and critical analysis of the difficulties relating to the political nature of this transition. In the 50s and 60s of last century, linear innovation models operated on the basis of a clear division of roles among the different actors in innovation and fundamentally economistic-based strategies. The following decades saw innovation policies progressively recognize the multi-dimensional and complex nature of innovation and the need to make adjustments, but always in explicit response to the competitiveness imperative. More recent RRI (Responsible Research and Innovation) strategy within the European Union, in contrast, demands opening up the whole innovation process (including values and motivations) to collective decision, i.e., approaching responsible innovation as inclusive innovation. This paper appraises this important development primarily on the basis of in-depth analysis of the main policy literature on innovation, and also on the grounds of related academic literature. As a result, we conclude that the bid for collaboration models cohabits constitutively with another set of dynamics aimed at strengthening centralized and prescriptive forms of innovation. In other words, that inclusionary or political eagerness represented through RRI must grapple with the strategic imperative of competitiveness and economic development. Hence, fundamental tension exists, which should be elucidated in light of the objectives, demands and considerations that are integrated, and cease to be integrated, in innovation dynamics and trajectories. Keywords: Inclusive innovation, Responsible Research and Innovation (RRI), Competitiveness, Institutional ecosystem

A secondary arrest is frequent in patients that recover spontaneous circulation after an out-of-hospital cardiac arrest (OHCA). Rearrest events are associated to worse patient outcomes, but little is known on the heart dynamics that lead to rearrest. The prediction of rearrest could help improve OHCA patient outcomes. The aim of this study was to develop a machine learning model to predict rearrest. A random forest classifier based on 21 heart rate variability (HRV) and electrocardiogram (ECG) features was designed. An analysis interval of 2 min after recovery of spontaneous circulation was used to compute the features. The model was trained and tested using a repeated cross-validation procedure, on a cohort of 162 OHCA patients (55 with rearrest). The median (interquartile range) sensitivity (rearrest) and specificity (no-rearrest) of the model were 67.3% (9.1%) and 67.3% (10.3%), respectively, with median areas under the receiver operating characteristics and the precision–recall curves of 0.69 and 0.53, respectively. This is the first machine learning model to predict rearrest, and would provide clinically valuable information to the clinician in an automated way.

Es reseña de: El atentado contra Carrero Blanco como lugar de (no-)memoria. Narraciones históricas y representaciones culturales [de] Patrick Eser y Stefan Peters (eds.), 2016.

Abstract Amyotrophic lateral sclerosis (ALS) is the most common fatal motor neuron disease in adults. Numerous studies indicate that ALS is a systemic disease that affects whole body physiology and metabolic homeostasis. Using a mouse model of the disease (SOD1G86R), we investigated muscle physiology and motor behavior with respect to muscle metabolic capacity. We found that at 65 days of age, an age described as asymptomatic, SOD1G86R mice presented with improved endurance capacity associated with an early inhibition in the capacity for glycolytic muscle to use glucose as a source of energy and a switch in fuel preference toward lipids. Indeed, in glycolytic muscles we showed progressive induction of pyruvate dehydrogenase kinase 4 expression. Phosphofructokinase 1 was inhibited, and the expression of lipid handling molecules was increased. This mechanism represents a chronic pathologic alteration in muscle metabolism that is exacerbated with disease progression. Further, inhibition of pyruvate dehydrogenase kinase 4 activity with dichloroacetate delayed symptom onset while improving mitochondrial dysfunction and ameliorating muscle denervation. In this study, we provide the first molecular basis for the particular sensitivity of glycolytic muscles to ALS pathology.

The article analyzes the variables that better explain the perceptions and attitudes of Europeans regarding science and their more recent scientific innovations. The cognitive deficit hypothesis proposes that social ambivalence arises because people have little scientific knowledge and misunderstands scientific and technical advances. The article agrees with the analytic role of contextual perspective, identifies ten myths linked to the traditional explanation of social perception of science, and proposes an alternative interpretation of the relationship between knowledge, institutional dynamics, and social trust.

La respuesta a los efectos no deseados de la tecnología ha consolidado un amplio conjunto de bases legales e instrumentos análiticos ligados a la evaluación de los riesgos. Este artículo plantea que buena parte de las controversias sobre los riesgos tecnológicos más que a la ausencia de su regulación se debe paradójicamente a la generalización de las políticas basadas en la ciencia. En el origen encontramos una concepción equívoca del conocimiento científico. La ciencia como gobierno de la sociedad requiere previamente una reflexión sobre las dinámicas del conocimiento y su gobierno.

We present the first rock magnetic study of archeologically-relevant chert samples from the Basque-Cantabrian basin (BCB) and surrounding regions, which was conducted in order to test the usefulness of non-destructive magnetic properties for assessing chert quality, distinguishing source areas, and identifying heated samples in the archeological record. Our results indicate that the studied BCB cherts are diamagnetic and have very low amounts of magnetic minerals. The only exception is the chert of Artxilondo, which has a mean positive magnetic susceptibility associated with larger concentrations of magnetic minerals. But even in this case, the magnetic susceptibility is within the lower range of other archeologically-relevant cherts elsewhere, which indicates that the studied BCB cherts can be considered as flint. The similar mean values for all magnetic properties, along with their associated large standard deviations, indicates that rock magnetic methods are of limited use for sourcing different types of flint except in some specific contexts involving the Artxilondo flint. With regards to the identification of chert heating in the archeological record, our results indicate only a minor magnetic enhancement of BCB natural flint samples upon heating, which we attribute to the low amount of non-silica impurities. In any case, the diamagnetic behavior of most BCB natural flints, along with the local use only of the Artxilondo type, suggests that any flint tool within the core of the BCB with positive magnetic susceptibility values is likely to have been subjected to heating for improving its knapping properties. Further studies are necessary to better identify the type, origin and grain size of magnetic minerals in BCB natural flints, and to apply non-destructive magnetic properties to flint tools in order to identify the use of heat treatment in the BCB archeological record.

Este trabajo comprende el principio de precaución como indicador de las tensiones en los estándares de gobierno. El principio de precaución no es una medida provisional y preventiva tomada por la falta de conocimientos y causado por la magnitud de los riesgos; no es una variante del análisis del riesgo. Antes bien, es un principio que genera reglas y normas orientadas a identificar espacios de incertidumbre y debatir acerca de lo que denominamos conocimiento relevante.

The automatic detection of pulse during out-of-hospital cardiac arrest (OHCA) is necessary for the early recognition of the arrest and the detection of return of spontaneous circulation (end of the arrest). The only signal available in every single defibrillator and valid for the detection of pulse is the electrocardiogram (ECG). In this study we propose two deep neural network (DNN) architectures to detect pulse using short ECG segments (5 s), i.e., to classify the rhythm into pulseless electrical activity (PEA) or pulse-generating rhythm (PR). A total of 3914 5-s ECG segments, 2372 PR and 1542 PEA, were extracted from 279 OHCA episodes. Data were partitioned patient-wise into training (80%) and test (20%) sets. The first DNN architecture was a fully convolutional neural network, and the second architecture added a recurrent layer to learn temporal dependencies. Both DNN architectures were tuned using Bayesian optimization, and the results for the test set were compared to state-of-the art PR/PEA discrimination algorithms based on machine learning and hand crafted features. The PR/PEA classifiers were evaluated in terms of sensitivity (Se) for PR, specificity (Sp) for PEA, and the balanced accuracy (BAC), the average of Se and Sp. The Se/Sp/BAC of the DNN architectures were 94.1%/92.9%/93.5% for the first one, and 95.5%/91.6%/93.5% for the second one. Both architectures improved the performance of state of the art methods by more than 1.5 points in BAC.

Growing evidence supports a link between fatty acid metabolism and amyotrophic lateral sclerosis (ALS). Here we determined the fatty acid composition of blood lipids to identify markers of disease progression and survival. We enrolled 117 patients from two clinical centers and 48 of these were age and gender matched with healthy volunteers. We extracted total lipids from serum and blood cells, and separated fatty acid methyl esters by gas chromatography. We measured circulating biochemical parameters indicative of the metabolic status. Association between fatty acid composition and clinical readouts was studied, including ALS functional rating scale-revised (ALSFRS-R), survival, disease duration, site of onset and body mass index. Palmitoleate (16:1) and oleate (18:1) levels, and stearoyl-CoA desaturase indices (16:1/16:0 and 18:1/18:0) significantly increased in blood cells from ALS patients compared to healthy controls. Palmitoleate levels and 16:1/16:0 ratio in blood cells, but not body mass index or leptin concentrations, negatively correlated with ALSFRS-R decline over a six-month period (p

En este artículo se analizan las filmaciones de las cuatro primeras ediciones del Aberri Eguna (Día de la Patria Vasca), celebradas en Bilbao en 1932, San Sebastián en 1933, Vitoria en 1934, y Pamplona en 1935. De este modo es posible conocer las iniciativas cinematográficas llevadas a cabo durante la II República por el Partido Nacionalista Vasco (PNV) y la evolución de esta festividad nacionalista en sus vertientes rituales, simbólicas, organizativas y políticas. El análisis de estas películas permite profundizar en el modo en el que el PNV empleó el cine y esta celebración para unir a la comunidad nacionalista en torno no sólo a unas ideas sino también a unos símbolos. Esta misma fiesta se convirtió rápidamente en un importante símbolo nacionalista vasco y actuó a su vez como catalizador de nuevas construcciones simbólicas. Las fuentes cinematográficas se emplean aquí por vez primera también para conocer más a fondo la historia del PNV, su organización y su liderazgo en la década de 1930.

BACKGROUND:Amyotrophic lateral sclerosis (ALS), the most frequent adult onset motor neuron disease, is associated with hypermetabolism linked to defects in muscle mitochondrial energy metabolism such as ATP depletion and increased oxygen consumption. It remains unknown whether muscle abnormalities in energy metabolism are causally involved in the destruction of neuromuscular junction (NMJ) and subsequent motor neuron degeneration during ALS. METHODOLOGY/PRINCIPAL FINDINGS:We studied transgenic mice with muscular overexpression of uncoupling protein 1 (UCP1), a potent mitochondrial uncoupler, as a model of muscle restricted hypermetabolism. These animals displayed age-dependent deterioration of the NMJ that correlated with progressive signs of denervation and a mild late-onset motor neuron pathology. NMJ regeneration and functional recovery were profoundly delayed following injury of the sciatic nerve and muscle mitochondrial uncoupling exacerbated the pathology of an ALS animal model. CONCLUSIONS/SIGNIFICANCE:These findings provide the proof of principle that a muscle restricted mitochondrial defect is sufficient to generate motor neuron degeneration and suggest that therapeutic strategies targeted at muscle metabolism might prove useful for motor neuron diseases.

Reseña de la obra de Enrique Gil Calvo aparecida en 2003, El miedo es el mensaje: Riesgo, incertidumbre y medios de comunicación. Madrid: Alianza. 320 p.

This study evaluated the efficacy of an active absorbent pad ( a PAD) in reducing microbial growth and enhancing the shelf life of rabbit meat stored in modified atmosphere packaging (MAP). Thigh muscles from 60 rabbits were used, divided into three dietary groups: a control group (CNT), a group supplemented with 5% extruded flaxseed (ELS5%), and a group with 3.5% extruded flaxseed and 0.2% Padina pavonica algae (LPP3.5%). Samples were packaged in MAP (70% O 2 , 30% CO 2 ) with either a conventional pad ( n PAD) or a PAD and analyzed at 1, 4, 7, 14, 21 days. Microbiological analysis revealed a significantly lower total viable count at 21 days in the ELS5% a PAD group. For coagulase-positive staphylococci, the CNT a PAD group showed lower microbial counts at both day 4 and day 21 ( p < 0.05). Enterobacteriaceae reductions were observed at 24 h post packaging in both the CNT a PAD and LPP3.5% a PAD groups and at day 14 in ELS5% a PAD. Lipid oxidation (TBARS) was also lower in a PAD samples, particularly in LPP3.5%, which remained below 1.5 mg MDA/kg compared to >2.5 mg MDA/kg in n PAD ( p < 0.05). Sensory attributes such as texture and color were better preserved with a PAD. These findings underscore the effectiveness of a PAD in MAP to control microbial growth, limit oxidation, and extend the shelf life of omega-3-enriched rabbit meat, providing a promising solution for functional meat product preservation.

This study investigated the effects of dietary supplementation with extruded linseed (ELS) and Padina pavonica algae extract (PP) on rabbit carcass and meat quality. Ninety-six rabbit carcasses from two production cycles were analyzed. In the first cycle (C1), rabbits were fed a control diet (1CNT), the same diet supplemented with 5% ELS (1ELS5%), and supplemented with 3.5% ELS and 0.2% PP (1LPP3.5%). In the second cycle (C2), the diets varied in composition and supplementation levels: a different control diet (2CNT), the same diet with 5% ELS (2ELS5%), and with 5% ELS and 0.2% PP (2LPP5%). Meat analyses were performed on Longissimus thoracis et lumborum (LTL) muscle for physical properties and on thigh meat (THM) for proximate composition, vitamin E, coenzyme-Q10, cholesterol, fatty acid profile, and mineral content. No significant differences in LTL physical quality were observed in C1, although LTL was brighter in C2 ( p < 0.001). THM in C2 had higher fat content ( p < 0.001). Dietary supplementation with ELS and PP extract significantly increased polyunsaturated fatty acids (n-3 PUFAs) and improved the n-6/n-3 ratio ( p < 0.001) in rabbit meat, demonstrating their positive impact on meat quality.

Heavy metals in the marine environment are significant contaminants that readily bioaccumulate in the tissues of aquatic organisms, particularly in filter-feeding animals such as bivalve molluscs. Human exposure to elevated concentrations of heavy metals, including essential elements such as Fe, Cu, and Zn, through the consumption of seafood can lead to various pathological effects. Research has demonstrated that among bivalve molluscs, mussels are the most effective indicators for monitoring marine pollution. Consequently, this study focused on the species Mytilus galloprovincialis to evaluate the levels of Al, As, Cd, Cr, Cu, Fe, Mg, Ni, Pb, and Zn in the two primary harvesting areas of Albania and to assess the associated human health risks from mussel consumption. The results revealed a concerning situation, particularly for Pb and Cd, with average concentrations of 2.15 μg/g and 4.14 μg/g, respectively, significantly exceeding the limits established by Regulation (EC) No. 915/2023. The levels of the other investigated elements also raised concerns, as only half of them were within the dietary intake values recommended by scientific authorities for weekly consumption of 250 g of mussels., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)

The nutritional, antimicrobial, and antioxidant properties of bovine colostrum (BC) have encouraged its use in animal nutrition as a functional food in recent years. Nonetheless, the potential implications of BC supplementation on meat quality remain to be thoroughly assessed. To address this, thirty-nine New Zealand White rabbits (n = 13/group) were fed different dietary regimens until slaughter.: commercial standard diet for the control group (C) and C with 2.5% and 5% w/w of BC for BC-2.5 and BC-5 groups, respectively. Rabbits were slaughtered at 91 days of age and meat quality, and sensory characteristics were evaluated at days 2 (48 h after slaughter), 5, and 10 of refrigerated storage at 4 °C. The addition of colostrum in the diet resulted in a reduction of the total viable count, albeit only at the highest concentration and at the final detection, whereas for Lactobacillus spp. and Pseudomonas spp., there was little or no effect. The colour coordinates showed no differences between the groups, but they varied over time according to diet. Some differences between groups emerged in the definition of sensory attributes but did not affect the overall liking and overall scores of individual descriptors. These results indicate that the use of colostrum in rabbit feeding does not significantly impart meat quality and sensory attributes, but the potential of this valuable by-product for the food industry needs further investigation., Competing Interests: Declaration of competing interest The authors declare no conflicts of interest in this article., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Rabbit breeding has many critical aspects related to reproduction, production, and animal welfare, which reduce its profitability as well as consumer attractiveness. Dietary supplementation with n-3 polyunsaturated fatty acids (PUFA) seems to be a good nutritional strategy to improve several aspects of rabbit breeding, enhance animal welfare and produce a new functional food considered healthy for human consumption. For this reason, the main available scientific research regarding the physiological effects of n-3 PUFA rich products supplemented to the rabbit diet will be reviewed. In particular, consequences on the reproductive performances of both doe and buck, the productive parameters, and the meat quality will be analysed., Competing Interests: We declare that we have no financial and personal relationships with other people or organizations that can inappropriately influence our work, and there is no professional or other personal interest of any nature or kind in any product, service and/or company that could be construed as influencing the content of this paper., (© 2023 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd.)

In June 2014, Albania was granted EU candidate status, thus starting a process of compliance with the membership criteria. In this context, a modern meat inspection approach in line with the European legislation was applied to a pig slaughterhouse in northern Albania in order to investigate the ante-mortem (AM) and post-mortem (PM) conditions and the relationship between these findings. For this purpose, 3930 pigs divided into 35 batches were evaluated over a 3-month period. The most frequent AM conditions recorded were tail lesions and dyspnea (9.1%), followed by skin (8.9%) and ear lesions (8.5%), while in the PM inspections, pleuritis was the most frequently observed condition (10.2%), followed by pneumonia (8.5%), liver alterations (5.7%), milk spot liver (3.8%), and pericarditis (3.3%). With the exception of liver alterations, the other PM lesions mentioned were positively associated with lesions on the ears (OR = 1.036; p < 0.001) and skin (OR = 1.026; p = 0.011) and dyspnea (OR = 1.021; p = 0.005), confirming the link between these variables and the health and welfare conditions of pigs on farms. Overall, the evidence that emerged from this Albanian slaughterhouse can be considered in line with other European contexts, especially in light of the considerable variability in the data present in the literature.

Bovine colostrum (BC) is rich in nutrients, antimicrobial, and antioxidant factors; for these reasons, it has been used as supplement in animal nutrition. However, its possible effects on meat quality have not been studied yet. Thirty-nine New Zealand White rabbits (n = 13/group) were assigned to three groups and fed until slaughter with a commercial standard diet, control group (C), and C supplemented with 2.5% and 5% (w/w) of BC (BC-2.5 and BC-5 groups, respectively). After slaughtering, the effect of dietary supplementation on microbiological and chemical characteristics of the rabbit loins was evaluated at 48 h postmortem (D0) and after 3 (D3) and 8 (D8) days of refrigerated storage. Results showed no difference in the microbiological parameters. In the supplemented groups, TBARS and TVBN values were lower and higher than in the C group, respectively (p < 0.01), and their fatty-acid profile was increased in SFA and decreased in MUFA (p < 0.05). In conclusion, research must continue to examine in depth the possible effects of BC byproduct reuse in animal nutrition on meat quality (e.g., antioxidant power, and physical and sensory characteristics).