17 results on '"András Bratincsák"'
Search Results
2. Editorial: Kawasaki disease: an ongoing enigma tangled with the appearance of MIS-C
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Andras Bratincsak and Marianna Fabi
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Kawasaki disease ,MIS-C multisystem inflammatory syndrome in children ,coronary artery dilation ,COVID-19 ,differential diagnosis ,Pediatrics ,RJ1-570 - Published
- 2023
- Full Text
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3. Temporal clustering of Kawasaki disease cases around the world
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Jennifer A. Burney, Laurel L. DeHaan, Chisato Shimizu, Emelia V. Bainto, Jane W. Newburger, Roberta L. DeBiasi, Samuel R. Dominguez, Michael A. Portman, Marian Melish, Andras Bratincsak, Marianna Fabi, Elena Corinaldesi, Jeong Jin Yu, Paul Gee, Naomi Kitano, Adriana H. Tremoulet, Daniel R. Cayan, Jane C. Burns, and the Kawasaki Disease Climate Study Group
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Medicine ,Science - Abstract
Abstract In a single-site study (San Diego, CA, USA), we previously showed that Kawasaki Disease (KD) cases cluster temporally in bursts of approximately 7 days. These clusters occurred more often than would be expected at random even after accounting for long-term trends and seasonality. This finding raised the question of whether other locations around the world experience similar temporal clusters of KD that might offer clues to disease etiology. Here we combine data from San Diego and nine additional sites around the world with hospitals that care for large numbers of KD patients, as well as two multi-hospital catchment regions. We found that across these sites, KD cases clustered at short time scales and there were anomalously long quiet periods with no cases. Both of these phenomena occurred more often than would be expected given local trends and seasonality. Additionally, we found unusually frequent temporal overlaps of KD clusters and quiet periods between pairs of sites. These findings suggest that regional and planetary range environmental influences create periods of higher or lower exposure to KD triggers that may offer clues to the etiology of KD.
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- 2021
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4. Osteoporosis treatment rates after hip fracture 2011–2019 in Hawaii: Undertreatment of men after hip fractures
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Luke Taylor, Chieko Kimata, Andrea M. Siu, Samantha N. Andrews, Prashant Purohit, Melissa Yamauchi, Andras Bratincsak, Russell Woo, Cass K. Nakasone, and Sian Yik Lim
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Hip fracture ,Hospitalization ,Osteoporosis ,Diseases of the musculoskeletal system ,RC925-935 - Abstract
Objectives: To investigate trends of osteoporosis treatment rates, and factors affecting osteoporosis treatment after hip fracture admission within a single health care system in Hawaii. Methods: A retrospective chart review was conducted of patients aged 50 years or older and hospitalized for hip fractures between January 1, 2011 and December 31, 2019 at Hawaii Pacific Health, a large health care system in Hawaii. We collected data on basic demographics and osteoporosis medication prescription from electronic medical records. We evaluated trends of osteoporosis treatment rates and performed logistic regression to determine factors associated with osteoporosis treatment. Results: The mean for treatment rates for osteoporosis from 2011 to 2019 was 17.2% (range 8.8%–26.0%). From 2011 to 2019 there was a small increase in treatment rates from 16.3% in 2011 to 24.1% in 2019. Men were less likely to receive osteoporosis treatment after admission for hip fracture. Patients discharged to a facility were more likely to receive osteoporosis treatment. As compared to women, men who had a hip fracture were less likely to receive dual-energy X-ray absorptiometry scan, and osteoporosis medication before hip fracture admission. Conclusions: The use of osteoporosis medication for secondary prevention after admission for hip fracture in Hawaii from 2011 to 2019 was low. However, there was a small increase in treatment rates from 2011 to 2019. Disparities in treatment of osteoporosis after hip fracture were noted in men. Significant work is needed to increase treatment rates further, and to address the disparity in osteoporosis treatment between men and women.
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- 2021
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5. Acute Adult-Onset Kawasaki Disease Complicated by Coronary Artery Aneurysms, Thrombosis, and ST-Segment Elevation Myocardial Infarction
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Andras Bratincsak, MD, PhD, Trudy M. Hong, MD, and Marian E. Melish, MD
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coronary aneurysm ,Kawasaki disease ,myocardial infarction ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
A 21-year-old man who had an initial misdiagnosis of chest wall cellulitis and sepsis presented to the emergency department with chest pain. Electrocardiogram demonstrated ST-segment elevation in the inferior leads. Cardiac catheterization identified diffuse aneurysmal dilation and thrombosis of the distal right coronary artery. Clinical signs were consistent with acute Kawasaki disease. (Level of Difficulty: Intermediate.)
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- 2021
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6. Elevated Levels of Pentraxin 3 Correlate With Neutrophilia and Coronary Artery Dilation During Acute Kawasaki Disease
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Lauren L. Ching, Vivek R. Nerurkar, Eunjung Lim, Ralph V. Shohet, Marian E. Melish, and Andras Bratincsak
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Kawasaki disease ,pentraxin 3 ,coronary artery lesions ,coronary artery dilatation ,coronary artery aneurysm ,IVIG resistance ,Pediatrics ,RJ1-570 - Abstract
Kawasaki disease (KD) is the leading cause of acquired pediatric heart disease in the developed world as 25–30% of untreated patients and at least 5% of treated patients will develop irreversible coronary artery lesions (CAL). Pentraxin-3 (PTX-3) has been well-studied in inflammatory diseases, particularly in cardiovascular diseases associated with vascular endothelial dysfunction. We hypothesized that PTX-3 plays an important role in the development of KD-associated CAL and investigated the circulating levels of PTX-3 in the serum of KD patients. Children with acute KD were followed from diagnosis through normalization of the clinical parameters of inflammation (convalescent phase). Serum samples were obtained and echocardiograms were conducted at several phases of the illness: acute [prior to intravenous immunoglobulin (IVIG) treatment], sub-acute (5–10 days after IVIG treatment), and convalescent (1–4 months after KD diagnosis). Seventy children were included in the final cohort of the study, of whom 26 (37%) presented with CAL and 18 (26%) developed IVIG resistance. The patients included in this study came from diverse ethnic backgrounds, mostly with mixed ancestry/ ethnicity. Significantly increased PTX-3 levels were observed during the acute phase of KD compared to the sub-acute and the convalescent phases. The PTX-3 levels during acute KD were significantly higher among KD patients with CAL compared to patients with normal coronary arteries (NCA). Also, the PTX-3 levels were significantly higher in patients with IVIG resistance. Furthermore, the PTX-3 levels were significantly higher in IVIG-resistant KD patients with CAL as compared to the NCA group. Moreover, the PTX-3 levels were significantly correlated to coronary artery z-score during acute KD and to neutrophil counts throughout KD progression regardless of coronary artery z-score. Elevated PTX-3 levels correlated to elevated neutrophil counts, a known source of PTX-3 in acute inflammation and an important player in the development of KD vasculitis. We, therefore, suggest PTX-3 as a novel factor in the development of KD-associated CAL and propose neutrophil-derived PTX-3 as contributing to KD vascular dysfunction.
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- 2020
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7. Electrocardiogram Standards for Children and Young Adults Using
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András, Bratincsák, Chieko, Kimata, Blair N, Limm-Chan, Kevin P, Vincent, Matthew R, Williams, and James C, Perry
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Adult ,Male ,Adolescent ,Body Surface Area ,Age Factors ,Infant, Newborn ,Action Potentials ,Infant ,Electrocardiography ,Young Adult ,Heart Conduction System ,Heart Rate ,Predictive Value of Tests ,Reference Values ,Child, Preschool ,Humans ,Female ,Child ,Retrospective Studies - Abstract
Normative ECG values for children are based on relatively few subjects and are not standardized, resulting in interpersonal variability of interpretation. Recent advances in digital technology allow a more quantitative, reproducible assessment of ECG variables. Our objective was to create the foundation of normative ECG standards in the young utilizingOne hundred two ECG variables were collected from a retrospective cohort of 27 085 study subjects with no known heart condition, ages 0 to 39 years. The cohort was divided into 16 age groups by sex. Median, interquartile range, and range were calculated for each variable adjusted to body surface area.Normative standards were developed for all 102 ECG variables including heart rate; P, R, and T axis; R-T axis deviation; PR interval, QRS duration, QT, and QTc interval; P, Q, R, S, and T amplitudes in 12 leads; as well as QRS and T wave integrals. IncrementalElectronically acquired ECG values based on the largest pediatric and young adult cohort ever compiled provide the first detailed, standardized, quantitative foundation of traditional and novel ECG variables. Expression of ECG variables by
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- 2020
8. Worsening of Functional Mitral Regurgitation from Septal Dyssynchrony Induced by Ventricular Pacing in Ebstein's Anomaly Undergoing Percutaneous Mitral Valve Repair
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Heajung L. Nguyen, Abdulfattah Saidi, Anwar Tandar, Elisa Rhee, András Bratincsák, William Van Alstine, Lindsay Koren, Kimberly Stoughton, José Negrón-Garcia, Anthony Ragheb, Hannah El-Sabrout, John W. Moore, Howaida el-Said, John P. Breinholt, Jason P. Glotzbach, Frederick G.P. Welt, Vikas Sharma, Kelsee Browning, Craig H. Selzman, David A. Bull, Holly Carveth, Marcella A. Calfon Press, Jamil A. Aboulhosn, Jeannette P. Lin, Peyman Benharash, and Eric H. Yang
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medicine.medical_specialty ,business.industry ,Ebstein's anomaly ,Internal medicine ,Cardiology ,Medicine ,Ventricular pacing ,business ,medicine.disease ,Functional mitral regurgitation ,Percutaneous Mitral Valve Repair - Abstract
Author(s): Nguyen, Heajung L; Calfon Press, Marcella A; Aboulhosn, Jamil A; Lin, Jeannette P; Benharash, Peyman; Yang, Eric H
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- 2017
9. Improvement in Pulmonary Function After Closure of Atrial Septal Defect in a Patient With Cystic Fibrosis
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Abdulfattah Saidi, Anwar Tandar, Elisa Rhee, András Bratincsák, William Van Alstine, Lindsay Koren, Kimberly Stoughton, José Negrón-Garcia, Anthony Ragheb, Hannah El-Sabrout, John W. Moore, Howaida el-Said, John P. Breinholt, Jason P. Glotzbach, Frederick G.P. Welt, Vikas Sharma, Kelsee Browning, Craig H. Selzman, David A. Bull, and Holly Carveth
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medicine.medical_specialty ,business.industry ,Closure (topology) ,Medicine ,business ,medicine.disease ,Cystic fibrosis ,Pulmonary function testing ,Surgery - Published
- 2017
10. Transforming growth factor α induces angiogenesis and neurogenesis following stroke
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Eva Mezey, András Bratincsák, Tal Shahar, Ildiko Szalayova, Ronen R. Leker, Riccardo Cassiani-Ingoni, and Zsuzsanna E. Tóth
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medicine.medical_specialty ,TGF alpha ,Angiogenesis ,business.industry ,General Neuroscience ,medicine.medical_treatment ,Neurogenesis ,medicine.disease ,Neuroprotection ,Neovascularization ,chemistry.chemical_compound ,Cytokine ,Endocrinology ,chemistry ,Internal medicine ,Immunology ,medicine ,medicine.symptom ,business ,Stroke ,Bromodeoxyuridine - Abstract
The cytokine transforming growth factor alpha (TGF alpha) has proangiogenic and proneurogenic effects and can potentially reduce infarct volumes. Therefore, we administered TGF alpha or vehicle directly into the area surrounding the infarct in female mice that received gender-mismatched bone marrow transplants from green fluorescent protein (GFP)-expressing males prior to undergoing permanent middle cerebral artery occlusion. Newborn cells were tracked with bromodeoxyuridine (BrdU) labeling and immunohistochemistry at 90 days after stroke onset. We also studied the ingress of bone marrow-derived cells into the ischemic brain to determine whether such cells contribute to angiogenesis or neurogenesis. Infarct volumes were measured at 90 days poststroke. The results show that TGF alpha led to significant increments in the number of newborn neurons and glia in the ischemic hemisphere. TGF alpha also led to significant increments in the number of bone marrow-derived cells entering into the ischemic hemisphere. Most of these cells did not label with BrdU and represented endothelial cells that incorporated into blood vessels in the infarct border zone. Our results also show that infarct size was significantly reduced in animals treated with TGF alpha compared with controls. These results suggest that TGF alpha can induce angiogenesis, neurogenesis and neuroprotection after stroke. At least part of the pro-angiogenic effect appears to be secondary to the incorporation of bone marrow-derived endothelial cells into blood vessels in the infarct border zone.
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- 2009
11. Disease Progression After Bone Marrow Transplantation in a Model of Multiple Sclerosis Is Associated With Chronic Microglial and Glial Progenitor Response
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Larry S. Sherman, Riccardo Cassiani-Ingoni, Tal Shahar, Jacqueline A. Quandt, Roland Martin, Tim Magnus, András Bratincsák, Mahendra S. Rao, Mark P. Mattson, Paolo A. Muraro, Jens Schmidt, Jaebong Huh, Susan Reichert-Scrivner, and Fabrizio Eusebi
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Encephalomyelitis, Autoimmune, Experimental ,Multiple Sclerosis ,Nerve Tissue Proteins ,Pathology and Forensic Medicine ,Mice ,Cellular and Molecular Neuroscience ,Antigens, CD ,Glial Fibrillary Acidic Protein ,Basic Helix-Loop-Helix Transcription Factors ,Animals ,Medicine ,Neuroinflammation ,Bone Marrow Transplantation ,Cell Proliferation ,Glycoproteins ,Microglia ,business.industry ,Stem Cells ,Experimental autoimmune encephalomyelitis ,Brain ,General Medicine ,Oligodendrocyte Transcription Factor 2 ,Flow Cytometry ,medicine.disease ,Peptide Fragments ,Mice, Inbred C57BL ,Disease Models, Animal ,medicine.anatomical_structure ,Spinal Cord ,Neurology ,Gliosis ,Immunology ,Disease Progression ,Neuroglia ,Myelin-Oligodendrocyte Glycoprotein ,Neurology (clinical) ,Bone marrow ,Stem cell ,medicine.symptom ,business ,Astrocyte - Abstract
Multiple sclerosis (MS), the most common nontraumatic cause of neurologic disability in young adults in economically developed countries, is characterized by inflammation, gliosis, demyelination, and neuronal degeneration in the CNS. Bone marrow transplantation (BMT) can suppress inflammatory disease in a majority of patients with MS but retards clinical progression only in patients treated in the early stages of the disease. Here, we applied BMT in a mouse model of neuroinflammation, experimental autoimmune encephalomyelitis (EAE), and investigated the kinetics of reconstitution of the immune system in the periphery and in the CNS using bone marrow cells isolated from syngeneic donors constitutively expressing green fluorescent protein. This approach allowed us to dissect the contribution of donor cells to the turnover of resident microglia and to the pathogenesis of observed disease relapses after BMT. BMT effectively blocked or delayed EAE development when mice were treated early in the course of the disease but was without effect in mice with chronic disease. We found that there is minimal overall replacement of host microglia with donor cells in the CNS and that newly transplanted cells do not appear to contribute to disease progression. In contrast, EAE relapses are accompanied by the robust activation of endogenous microglial and macroglial cells, which further involves the maturation of endogenous Olig2 glial progenitor cells into reactive astrocytes through the cytoplasmic translocation of Olig2 and the expression of CD44 on the cellular membrane. The observed maturation of large numbers of reactive astrocytes from glial progenitors and the chronic activation of host microglial cells have relevance for our understanding of the resident glial response to inflammatory injury in the CNS. Our data indicate that reactivation of a local inflammatory process after BMT is sustained predominantly by endogenous microglia/macrophages.
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- 2007
12. Low dose tissue plasminogen activator treatment for vascular thrombosis following cardiac catheterization in children: a single center experience
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András, Bratincsák, John W, Moore, and Howaida G, El-Said
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Venous Thrombosis ,Cardiac Catheterization ,Time Factors ,Age Factors ,Thrombosis ,Femoral Vein ,California ,Femoral Artery ,Treatment Outcome ,Fibrinolytic Agents ,Health Care Surveys ,Tissue Plasminogen Activator ,Humans ,Drug Dosage Calculations ,Thrombolytic Therapy ,Ultrasonography, Doppler, Color ,Retrospective Studies - Abstract
To assess the efficacy and safety of low dose tissue plasminogen activator (tPA) therapy in children with vascular thrombosis following cardiac catheterization.Currently, heparin is the standard of care for vascular thrombosis, however, it may not be efficacious in dissolving existing thrombi. Although tPA is an accepted thrombolytic therapy for life-threatening thrombosis in children, it is not a well-established treatment for the thrombosis of femoral vessels following cardiac catheterization.A retrospective analysis was performed from 1/1/2009 until 6/30/2011 at Rady Children's Hospital-San Diego (RCHSD) reviewing all cases with venous or arterial thrombosis at the vascular access site following cardiac catheterization, and a survey was conducted among the Congenital Cardiovascular Interventional Study Consortium members regarding their experience in tPA therapy.At RCHSD, out of 1,155 catheterizations, 12 children developed femoral thrombosis. In three children, where 12-h heparin therapy was unsuccessful in resolving thrombosis, we used low-dose tPA according to the following regime: 0.05 mg/kg/h for 30 min, followed by 0.1 mg/kg/h for 4 h. We achieved thrombolysis in all cases evidenced by repeat ultrasound and return of palpable pulse with normal limb circulation. No complications were encountered. The survey confirmed that adult tPA dose (0.1-0.5 mg/kg/h) has 100% efficacy in resolving thrombi in children with vascular thrombosis, however, the rate of serious complications was not negligible (15%).tPA is an efficacious therapy to dissolve thrombi that developed as a complication of cardiac catheterization in children. The rate of complications due to tPA may be reduced using a lower dose: 0.05-0.1 mg/kg/h.
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- 2012
13. The combination of granulocyte colony-stimulating factor and stem cell factor significantly increases the number of bone marrow-derived endothelial cells in brains of mice following cerebral ischemia
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Tal Shahar, Krisztián Németh, András Bratincsák, Ildiko Szalayova, Ronen R. Leker, Balázs Mayer, Brook Asemenew, Zsuzsanna E. Tóth, Alissa Parmelee, Sandra Pastorino, Sharon Key, and Eva Mezey
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Pathology ,medicine.medical_specialty ,Hematopoiesis and Stem Cells ,Angiogenesis ,medicine.medical_treatment ,Immunology ,Green Fluorescent Proteins ,Neovascularization, Physiologic ,Stem cell factor ,Biochemistry ,Brain Ischemia ,Mice ,Granulocyte Colony-Stimulating Factor ,medicine ,Animals ,Bone Marrow Transplantation ,Stem Cell Factor ,business.industry ,Endothelial Cells ,Infarction, Middle Cerebral Artery ,Cell Biology ,Hematology ,Recovery of Function ,Granulocyte colony-stimulating factor ,Endothelial stem cell ,Mice, Inbred C57BL ,Haematopoiesis ,Cytokine ,medicine.anatomical_structure ,Cancer research ,Drug Therapy, Combination ,Female ,Bone marrow ,Stem cell ,business ,Cell Division - Abstract
Granulocyte colony-stimulating factor (G-CSF) induces proliferation of bone marrow–derived cells. G-CSF is neuroprotective after experimental brain injury, but the mechanisms involved remain unclear. Stem cell factor (SCF) is a cytokine important for the survival and differentiation of hematopoietic stem cells. Its receptor (c-kit or CD117) is present in some endothelial cells. We aimed to determine whether the combination of G-CSF/SCF induces angiogenesis in the central nervous system by promoting entry of endothelial precursors into the injured brain and causing them to proliferate there. We induced permanent middle cerebral artery occlusion in female mice that previously underwent sex-mismatched bone marrow transplantation from enhanced green fluorescent protein (EGFP)–expressing mice. G-CSF/SCF treatment reduced infarct volumes by more than 50% and resulted in a 1.5-fold increase in vessel formation in mice with stroke, a large percentage of which contain endothelial cells of bone marrow origin. Most cells entering the brain maintained their bone marrow identity and did not transdifferentiate into neural cells. G-CSF/SCF treatment also led to a 2-fold increase in the number of newborn cells in the ischemic hemisphere. These findings suggest that G-CSF/SCF treatment might help recovery through induction of bone marrow–derived angiogenesis, thus improving neuronal survival and functional outcome.
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- 2008
14. Spatial and temporal activation of brain regions in hibernation: c-fos expression during the hibernation bout in thirteen-lined ground squirrel
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David P. McMullen, Miklos Palkovits, Shinichi Miyake, András Bratincsák, John M. Hallenbeck, and Zsuzsanna Tóth
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Hibernation ,medicine.medical_specialty ,Suprachiasmatic nucleus ,Reverse Transcriptase Polymerase Chain Reaction ,General Neuroscience ,Brain ,Sciuridae ,Torpor ,Biology ,Thermoregulation ,Article ,Circadian Rhythm ,Preoptic area ,Endocrinology ,Arcuate nucleus ,Hypothalamus ,Internal medicine ,medicine ,Animals ,Choroid plexus ,RNA, Messenger ,Arousal ,Proto-Oncogene Proteins c-fos ,In Situ Hybridization - Abstract
Hibernation results in dramatic changes in body temperature and metabolism; however, the central nervous system remains active during deep torpor. By cloning c-fos cDNA from the 13-lined ground squirrel (Spermophilus tridecemlineatus) and using squirrel c-fos mRNA probe for in situ hybridization histochemistry, we systematically analyzed and identified specific brain regions that were activated during six different phases of the hibernation bout. During entrance into torpor, we detected activation of the ventrolateral subdivision of the medial preoptic area ('thermoregulatory center'), and the reticular thalamic nucleus, which is known to inhibit the somatomotor cortex. During torpor, c-fos expression in the cortex was suppressed while the reticular thalamic nucleus remained uniformly active. Throughout torpor the suprachiasmatic nucleus ('biological clock') showed increasing activity, likely participating in phase-change regulation of the hibernation bout. Interestingly, during torpor very strong c-fos activation was seen in the epithelial cells of the choroid plexus and in tanycytes at the third ventricle, both peaking near the beginning of arousal. In arousal, activity of the suprachiasmatic and reticular thalamic nuclei and choroid epithelial cells diminished, while ependymal cells in the lateral and fourth ventricles showed stronger activity. Increasing body temperature during arousal was driven by the activation of neurons in the medial part of the preoptic area. In interbout awake animals, we demonstrated the activation of hypothalamic neurons located in the arcuate nucleus and the dorsolateral hypothalamus, areas involved in food intake. Our observations indicate that the hibernation bout is closely regulated and orchestrated by specific regions of the central nervous system. J. Comp. Neurol. 505:443-458, 2007. (c) 2007 Wiley-Liss, Inc.
- Published
- 2007
15. CD45-positive blood cells give rise to uterine epithelial cells in mice
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Zsuzsanna E. Tóth, Riccardo Cassiani-Ingoni, Ildiko Szalayova, András Bratincsák, Michael J. Brownstein, James Pickel, Eva Mezey, Sharon Key, Sandra Pastorino, and Krisztián Németh
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Aging ,Stromal cell ,CD34 ,Mice, Transgenic ,Biology ,Mice ,Pregnancy ,Animals ,Progenitor cell ,Interleukin 3 ,Uterus ,Cell Differentiation ,Epithelial Cells ,Cell Biology ,Hematopoietic Stem Cells ,Cell biology ,Endothelial stem cell ,Mice, Inbred C57BL ,Amniotic epithelial cells ,Immunology ,Molecular Medicine ,Leukocyte Common Antigens ,Female ,Stem cell ,Developmental Biology ,Adult stem cell - Abstract
The uterine endometrium is composed of epithelial and stromal cells, which undergo extensive degeneration and regeneration in every estrous cycle, and dramatic changes occur during pregnancy. The high turnover of cells requires a correspondingly high level of cell division by progenitor cells in the uterus, but the character and source of these cells remain obscure. In the present study, using a novel transgenic mouse, we showed that CD45-positive hematopoietic progenitor cells colonize the uterine epithelium and that in pregnancy more than 80% of the epithelium can derive from these cells. Since we also found green fluorescent protein (GFP)-positive uterine endothelial cells in long-term GFP bone marrow-transplanted mice, we conclude that circulating CD45+ cells play an important role in regenerating the uterine epithelium. Disclosure of potential conflicts of interest is found at the end of this article.
- Published
- 2007
16. Using brain slice cultures of mouse brain to assess the effect of growth factors on differentiation of bone marrow derived stem cells
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András, Bratincsák, Anna, Lonyai, Tal, Shahar, Arne, Hansen, Zsuzsanna E, Tóth, and Eva, Mezey
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Tissue Culture Techniques ,Mice ,Time Factors ,Epidermal Growth Factor ,Stem Cells ,Green Fluorescent Proteins ,Animals ,Brain ,Bone Marrow Cells ,Cell Differentiation ,Chemokines, CXC ,Immunohistochemistry ,Chemokine CXCL12 - Abstract
Bone marrow derived stem cells (BMDSCs) have been reported to form neurons and supportive cells in the brain. We describe a technique that combines the simplicity of in vitro studies with many of the advantages of in vivo experiments. We cultured mouse brain slices, deposited GFP-tagged BMDSCs evenly distributed on their surfaces, and then added test factors to the culture medium. Addition of both SDF-1 and EGF resulted in morphological changes of BMDSC and in the induction of islet-1, a marker of neuroepithelial progenitors. We conclude that organotypic tissue culture (OTC) may allow us to detect the effects of exogenous factors on the differentiation of BMDSCs (or any other type of stem cells) in an environment that may resemble the CNS after brain injury. Once such factors have been identified they could be evaluated for tissue regeneration in more complex, whole animal models.
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- 2007
17. Comment on 'Failure of Bone Marrow Cells to Transdifferentiate into Neural Cells in Vivo'
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András Bratincsák, Ildiko Szalayova, Tal Shahar, Sharon Key, Eva Mezey, Andras Nagy, and J. Baffi
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Multidisciplinary ,medicine.anatomical_structure ,Bone marrow transplantation ,Microglia ,Chemistry ,In vivo ,Cellular differentiation ,medicine.medical_treatment ,Immunology ,medicine ,Bone marrow ,Hematopoietic stem cell transplantation ,Cell biology - Abstract
Castro et al . ([1][1]) reported that bone marrow cells (BMCs) fail to generate neural cells in vivo. The bone marrow they injected came from mice in which LacZ expression was driven by a widely expressed trapped promoter. In principle, such cells and their progeny should readily be detected by
- Published
- 2003
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