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The use of bioprocess to convert low valued biomass or agroindustrial byproducts into high-value chemicals is an emerging area. However, laboratories usually use outdated analytical techniques to identify bioproducts, losing valuable information, such as the identification of unexpected compounds. In this work, we presented a successful systematic and modern analytical approach based on direct infusion mass spectrometry (DIMS) for an automated high-throughput screening of untargeted compounds from glycerin bioconversion process. We describe advantages of DIMS and its combined application with chemometrics towards an untargeted metabolomics approach to analyze several samples in a short time (11 samples per h). Three batches with 34 samples from the bioconversion of glycerin using several filamentous fungi strains were analyzed. Batch 347 was selected as promising, since high value chemicals, such as amide, phenolic and acid compounds, were identified. The platform presented was fast, robust, and versatile, then it could be applied to different bioprocesses.

Extracellular β-1,3-glucanase was produced by Trichoderma harzianum Rifai cultivated in the Agaricus blazei (Agaricus brasiliensis) extract as a substrate in submerged fermentation. A 2²-central composite factorial design was developed using the time of culture (x1/day) and Agaricus blazei extract concentration (x2/(g/L)) as variables, and the results were analyzed using response surface methodology (RSM). The results showed that the Agaricus blazei extract concentration was the most important variable in the production of β-1,3-glucanase, and the maximum β-1,3-glucanase activity (0.77 U/mL) was obtained in one day of cultivation. The β-glucan present in the cell wall of Agaricus blazei mushroom proved to be a good substrate for inducing the production of specific β-1,3-glucanase by Trichoderma harzianum Rifai., Ekstrakt gljive Agaricus blazei (Agaricus brasiliensis) upotrijebljen je kao supstrat za proizvodnju β-1,3-glukanaze submerznim uzgojem plijesni Trichoderma harzianum Rifai. Centralno složeni 22 faktorijalni dizajn razvijen je primjenom ovih varijabla: vrijeme uzgoja (x1/dan) i koncentracija ekstrakta Agaricus blazei (x2/(g/L)), a rezultati su analizirani metodom odzivnih površina (RSM). Rezultati pokazuju da je koncentracija ekstrakta Agaricus blazei najvažnija varijabla za proizvodnju β-1,3-glukanaze, a maksimalna je aktivnost enzima od 0,77 U/mL dobivena nakon prvoga dana uzgoja. β-Glukan u staničnoj stijenci gljive Agaricus blazei dobar je supstrat za proizvodnju specifične β-1,3-glukanaze s pomoću Trichoderma harzianum Rifai.