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1. Human Campylobacter spp. infections in Italy

Author

Zerbato, Verena, Di Bella, Stefano, Pol, Riccardo, Luzzati, Roberto, Sanson, Gianfranco, Ambretti, Simone, Andreoni, Stefano, Aschbacher, Richard, Bernardo, Mariano, Bielli, Alessandra, Brigante, Gioconda, Busetti, Marina, Camarlinghi, Giulio, Carcione, Davide, Carducci, Antonella, Clementi, Nicola, Carretto, Edoardo, Chilleri, Chiara, Codda, Giulia, Consonni, Alessandra, Costantino, Venera, Cortazzo, Venere, Di Santolo, Manuela, Dodaro, Saveria, Fiori, Barbara, García-Fernández, Aurora, Foschi, Claudio, Gobbato, Elisa, Greco, Francesca, La Ragione, Roberto Marcello, Mancini, Nicasio, Maraolo, Alberto Enrico, Marchese, Anna, Marcuccio, Daniela, Marrollo, Roberta, Mauri, Carola, Mazzariol, Annarita, Morroni, Gianluca, Mosca, Adriana, Nigrisoli, Giacomo, Pagani, Elisabetta, Parisio, Eva Maria, Pollini, Simona, Sarti, Mario, Sorrentino, Annarita, Trotta, Domenico, Villa, Laura, Vismara, Chiara, and Principe, Luigi

Published
2024
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2. Hepatitis C Virus as a Possible Helper Virus in Human Hepatitis Delta Virus Infection.

Author

Crobu, Maria Grazia, Ravanini, Paolo, Impaloni, Clotilde, Martello, Claudia, Bargiacchi, Olivia, Di Domenico, Christian, Faolotto, Giulia, Macaluso, Paola, Mercandino, Alessio, Riggi, Miriam, Quaglia, Vittorio, Andreoni, Stefano, Pirisi, Mario, and Smirne, Carlo

Subjects
HEPATITIS D virus, HEPATITIS C virus, HEPATITIS B virus, HEPATITIS B
Abstract

Previous studies reported that the hepatitis C virus (HCV) could help disseminate the hepatitis D virus (HDV) in vivo through the unrelated hepatitis B virus (HBV), but with essentially inconclusive results. To try to shed light on this still-debated topic, 146 anti-HCV-positive subjects (of whom 91 HCV/HIV co-infected, and 43 with prior HCV eradication) were screened for anti-HDV antibodies (anti-HD), after careful selection for negativity to any serologic or virologic marker of current or past HBV infection. One single HCV/HIV co-infected patient (0.7%) tested highly positive for anti-HD, but with no positive HDV-RNA. Her husband, in turn, was a HCV/HIV co-infected subject with a previous contact with HBV. While conducting a thorough review of the relevant literature, the authors attempted to exhaustively describe the medical history of both the anti-HD-positive patient and her partner, believing it to be the key to dissecting the possible complex mechanisms of HDV transmission from one subject to another, and speculating that in the present case, it may have been HCV itself that behaved as an HDV helper virus. In conclusion, this preliminary research, while needing further validation in large prospective studies, provided some further evidence of a role of HCV in HDV dissemination in humans. [ABSTRACT FROM AUTHOR]

Published
2024
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3. Multicenter Italian Study on “In Vitro Activities” of Isavuconazole, Voriconazole, Amphotericin B, and Caspofungin for Aspergillus Species: Comparison between SensititreTM YeastOneTM and MIC Test Strip

Author

Lo Cascio, Giuliana, primary, Bazaj, Alda, additional, Trovato, Laura, additional, Sanna, Silvana, additional, Andreoni, Stefano, additional, Blasi, Elisabetta, additional, Conte, Marco, additional, Fazii, Paolo, additional, Oliva, Ester, additional, Lepera, Valentina, additional, Lombardi, Gianluigi, additional, and Farina, Claudio, additional

Published
2022
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4. Multicenter Italian Study on “In Vitro Activities” of Isavuconazole, Voriconazole, Amphotericin B, and Caspofungin for Aspergillus Species: Comparison between SensititreTM YeastOneTM and MIC Test Strip

Author

Lo Cascio,Giuliana, Bazaj,Alda, Trovato,Laura, Sanna,Silvana, Andreoni,Stefano, Blasi,Elisabetta, Conte,Marco, Fazii,Paolo, Oliva,Ester, Lepera,Valentina, Lombardi,Gianluigi, Farina,Claudio, Lo Cascio,Giuliana, Bazaj,Alda, Trovato,Laura, Sanna,Silvana, Andreoni,Stefano, Blasi,Elisabetta, Conte,Marco, Fazii,Paolo, Oliva,Ester, Lepera,Valentina, Lombardi,Gianluigi, and Farina,Claudio

Abstract

Giuliana Lo Cascio,1– 3 Alda Bazaj,1 Laura Trovato,2,4 Silvana Sanna,2,5 Stefano Andreoni,2,6 Elisabetta Blasi,2,7 Marco Conte,2,8 Paolo Fazii,2,9 Ester Oliva,10 Valentina Lepera,11 Gianluigi Lombardi,2,11 Claudio Farina2,10 1Clinical Microbiology and Virology Unit, Azienda Ospedaliera Universitaria Integrata, Verona, Italy; 2Medical Mycology Committee, Italian Society of Clinical Microbiologist, Milan, Italy; 3Clinical Microbiology and Virology Unit, Azienda USL, Piacenza, Italy; 4Clinical Microbiology, Azienda Ospedaliera Universitaria- Policlinico Vittorio Emanuele, Catania, Italy; 5Microbiology and Virology Unit, Azienda Ospedaliera Universitaria, Sassari, Italy; 6Microbiology and Virology Unit, Azienda Ospedaliero Universitaria Maggiore della Carità , Novara, Italy; 7Clinical Microbiology, Azienda Ospedaliero-Universitaria, Policlinico di Modena, Modena, Italy; 8Microbiology and Virology Unit, Grande Ospedale Metropolitano Bianchi- Melacrino- Morelli, Reggio, Calabria, Italy; 9Clinical Microbiology and Virology P.O. Spirito Santo, Pescara, Italy; 10Clinical Microbiology and Virology Unit, ASST Papa Giovanni XXIII, Bergamo, Italy; 11Clinical Microbiology, ASST Grande Ospedale Metropolitano Niguarda, Milan, ItalyCorrespondence: Giuliana Lo Cascio, Email g.locascio@ausl.pc.itAbstract: In this study the activity of Isavuconazole, Voriconazole, Amphotericin B, and Caspofungin against 224 clinical isolates of Aspergillus spp. originating from seven Italian hospitals, was comparatively evaluated with two commercial antifungal susceptibility tests (AST): SensititreTM YeastOneTM (SYO) and MIC Test Strip. More attention was focused on Isavuconazole activity, given the new introduction of the drug in widely distributed antifungal susceptibilities methods in the clinical microbiology lab. The minimum inhibitory concentrations of antifungal drug that can inhibit the growth of pathogen by 90% (MIC90) for Isavuconazole detected by SYO were 0.5, 1, 0.25, and 2 μg/mL for As

Published
2022

5. Multicenter Italian Study on "In Vitro Activities" of Isavuconazole, Voriconazole, Amphotericin B, and Caspofungin for Aspergillus Species: Comparison between SensititreTM YeastOneTM and MIC Test Strip.

Author

Cascio, Giuliana Lo, Bazaj, Alda, Trovato, Laura, Sanna, Silvana, Andreoni, Stefano, Blasi, Elisabetta, Conte, Marco, Fazii, Paolo, Oliva, Ester, Lepera, Valentina, Lombardi, Gianluigi, and Farina, Claudio

Subjects
ASPERGILLUS, AMPHOTERICIN B, CASPOFUNGIN, VORICONAZOLE, ASPERGILLUS fumigatus, ASPERGILLUS niger
Abstract

In this study the activity of Isavuconazole, Voriconazole, Amphotericin B, and Caspofungin against 224 clinical isolates of Aspergillus spp. originating from seven Italian hospitals, was comparatively evaluated with two commercial antifungal susceptibility tests (AST): SensititreTM YeastOneTM (SYO) and MIC Test Strip. More attention was focused on Isavuconazole activity, given the new introduction of the drug in widely distributed antifungal susceptibilities methods in the clinical microbiology lab. The minimum inhibitory concentrations of antifungal drug that can inhibit the growth of pathogen by 90% (MIC90) for Isavuconazole detected by SYO were 0.5, 1, 0.25, and 2 μg/mL for Aspergillus fumigatus, Aspergillus flavus, Aspergillus terreus, and Aspergillus niger, respectively, whilst they were 0.25, 0.25, 0.5, and 0.75 μg/mL by MIC Test Strip. Essential agreement between the two tested methods for Isavuconazole is 70% for all the species tested, 75.7% for A. fumigatus, 45.2% for A. flavus, 90.6% for A. terreus, and 40% for A. niger. Although the tested strains do not express any phenotypic resistance, MIC results were quite different if tested with microdilution broth or gradient agar method. This is the first Italian multicenter report on Isavuconazole MIC obtained employing the widely used SensititreTM Yeast OneTM (SYO) and MIC Test Strip on clinical isolates of Aspergillus. [ABSTRACT FROM AUTHOR]

Published
2022
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7. Control de trayectoria mediante LIDAR de un vehículo omnidireccional

Author

Andreoni, Stefano

Subjects
LIDAR, Raspberry, Robot, Mecanum wheel robot, Omnidireccional robot, Vehículo omnidireccional, Arduino, Kalman filter, Mecanum, Filtro de Kalman, Máster Universitario en Ingeniería Mecatrónica-Màster Universitari en Enginyeria Mecatrònica, INGENIERIA DE SISTEMAS Y AUTOMATICA
Abstract

[ES] El objetivo del trabajo es la puesta en marcha de un sistema de control de trayectoria de un vehículo equipado con ruedas mecanum. Para el posicionamiento, necesario para determinar la trayectoria seguida por el vehículo, se va a emplear la información proporcionada por un dispositivo LIDAR, incorporado al propio vehículo. Para el control de velocidad angular de las ruedas, que determina el movimiento longitudinal, transversal y angular del vehículo, se empleará Arduino como dispositivo de control. Para el procesado de la información proporcionada por el LIDAR se empleará Raspberry como dispositivo de procesamiento., [EN] The aim of this work is to implement a trajectory control system for a mecanum wheels vehicle . To estimate the position, which is necessary to the vehicle to follow a trajectory ,will be used the information provided by a LIDAR. For the speed control of the wheels, which determines the longitudinal, transversal and angular movement of the vehicle, Arduino will be used as the control device. Raspberry will be used as the processing device for processing LIDAR data.

Published
2021

8. Control de trayectoria mediante LIDAR de un vehículo omnidireccional

Author

Casanova Calvo, Vicente Fermín, Universitat Politècnica de València. Departamento de Ingeniería de Sistemas y Automática - Departament d'Enginyeria de Sistemes i Automàtica, Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería del Diseño - Escola Tècnica Superior d'Enginyeria del Disseny, Andreoni, Stefano, Casanova Calvo, Vicente Fermín, Universitat Politècnica de València. Departamento de Ingeniería de Sistemas y Automática - Departament d'Enginyeria de Sistemes i Automàtica, Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería del Diseño - Escola Tècnica Superior d'Enginyeria del Disseny, and Andreoni, Stefano

Abstract

[ES] El objetivo del trabajo es la puesta en marcha de un sistema de control de trayectoria de un vehículo equipado con ruedas mecanum. Para el posicionamiento, necesario para determinar la trayectoria seguida por el vehículo, se va a emplear la información proporcionada por un dispositivo LIDAR, incorporado al propio vehículo. Para el control de velocidad angular de las ruedas, que determina el movimiento longitudinal, transversal y angular del vehículo, se empleará Arduino como dispositivo de control. Para el procesado de la información proporcionada por el LIDAR se empleará Raspberry como dispositivo de procesamiento., [EN] The aim of this work is to implement a trajectory control system for a mecanum wheels vehicle . To estimate the position, which is necessary to the vehicle to follow a trajectory ,will be used the information provided by a LIDAR. For the speed control of the wheels, which determines the longitudinal, transversal and angular movement of the vehicle, Arduino will be used as the control device. Raspberry will be used as the processing device for processing LIDAR data.

Published
2021

9. Superficial and subcutaneous mycoses

Author

Fazii Paolo, Andreoni Stefano, Farina Claudio, Laura Trovato, Blasi Elisabetta, Sanna Silvana, Giuliana Lo Cascio, Lombardi Gianluigi, and Marco Conte

Subjects
integumentary system, lcsh:QR1-502, General Medicine, Mycoses, subcutaneous, superficial, protocol, lcsh:Microbiology
Abstract

Tegument mycoses are classified into three groups: 1) Superficial skin mycoses or superficial mycoses: infections sustained by fungi limited to the skin horny layer or the hair extrafollicular portion, without a significant inflammatory response in the host. 2) Skin mycoses (dermatophytosis) sensu stricto: fungal infections in which skin and its annexes, at the level of the keratinized layers, are involved with an evident immune response by the host 3) Mycosis of the subcutaneous tissue: fungal infections that mainly affect the subcutaneous tissue and secondarily, by contiguity, skin, bones and other tissues, with a strong immune response by the host [...].

Published
2020

10. In Island erworbene Hautinfektion

Author

Gironi, Laura Cristina, primary, Cammarata, Edoardo, additional, Camaggi, Anna, additional, Brustia, Diego, additional, Giacalone, Angela, additional, Andreoni, Stefano, additional, Giorgione, Roberto, additional, Boggio, Paolo, additional, Astolfi, Stefano, additional, Tiberio, Rossana, additional, and Savoia, Paola, additional

Published
2021
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14. Chest CT accuracy in diagnosing COVID-19 during the peak of the Italian epidemic: A retrospective correlation with RT-PCR testing and analysis of discordant cases

Author

Falaschi, Zeno, primary, Danna, Pietro S.C., additional, Arioli, Roberto, additional, Pasché, Alessio, additional, Zagaria, Domenico, additional, Percivale, Ilaria, additional, Tricca, Stefano, additional, Barini, Michela, additional, Aquilini, Ferruccio, additional, Andreoni, Stefano, additional, and Carriero, Alessandro, additional

Published
2020
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15. Granulomatous skin infection from Iceland

Author

Gironi, Laura Cristina, primary, Cammarata, Edoardo, additional, Camaggi, Anna, additional, Brustia, Diego, additional, Giacalone, Angela, additional, Andreoni, Stefano, additional, Giorgione, Roberto, additional, Boggio, Paolo, additional, Astolfi, Stefano, additional, Tiberio, Rossana, additional, and Savoia, Paola, additional

Published
2020
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16. Superficial and subcutaneous mycoses

Author

Lombardi, Gianluigi, primary, Lo Cascio, Giuliana, additional, Andreoni, Stefano, additional, Blasi, Elisabetta, additional, Conte, Marco, additional, Farina, Claudio, additional, Fazii, Paolo, additional, Sanna, Silvana, additional, and Trovato, Laura, additional

Published
2020
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17. Disseminated ulcers with sporotrichoid distribution

Author

Veronese, Federica, primary, Molinari, Rossella, additional, Astolfi, Stefano, additional, Saini, Luigia, additional, Nicali, Roberta, additional, Camaggi, Anna, additional, Andreoni, Stefano, additional, Balbo, Piero Emilio, additional, and Savoia, Paola, additional

Published
2020
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18. Disseminierte Ulzera mit sporotrichoider Ausbreitung

Author

Veronese, Federica, primary, Molinari, Rossella, additional, Astolfi, Stefano, additional, Saini, Luigia, additional, Nicali, Roberta, additional, Camaggi, Anna, additional, Andreoni, Stefano, additional, Balbo, Piero Emilio, additional, and Savoia, Paola, additional

Published
2020
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21. Antifungal susceptibility of invasive yeast isolates in Italy: the GISIA3 study in critically ill patients

Author

Mussap Michele, Manso Ester, Lombardi Gianluigi, Cascio Giuliana, Farina Claudio, Brigante Gioconda, Andreoni Stefano, Amato Gerardino, Iatta Roberta, Borghi Elisa, Morace Giulia, Pecile Patrizia, Rigoli Roberto, Tangorra Elisabetta, Valmarin Maria, and Montagna Maria

Subjects
Infectious and parasitic diseases, RC109-216
Abstract

Abstract Background Yeasts are a common cause of invasive fungal infections in critically ill patients. Antifungal susceptibility testing results of clinically significant fungal strains are of interest to physicians, enabling them to adopt appropriate strategies for empiric and prophylactic therapies. We investigated the antifungal susceptibility of yeasts isolated over a 2-year period from hospitalised patients with invasive yeast infections. Methods 638 yeasts were isolated from the blood, central venous catheters and sterile fluids of 578 patients on general and surgical intensive care units and surgical wards. Etest strips and Sensititre panels were used to test the susceptibility of the isolates to amphotericin B, anidulafungin, caspofungin, fluconazole, itraconazole, posaconazole and voriconazole in 13 laboratories centres (LC) and two co-ordinating centres (CC). The Clinical and Laboratory Standards Institute (CLSI) reference broth microdilution method was used at the CCs for comparison. Results Etest and Sensititre (LC/CC) MIC90 values were, respectively: amphotericin B 0.5/0.38, 1/1 mg/L; anidulafungin 2/1.5 and 1/1 mg/L; caspofungin 1/0.75 and 0.5/0.5 mg/L; fluconazole 12/8 and 16/16 mg/L; itraconazole 1/1.5, 0.5/0.5 mg/L; posaconazole 0.5 mg/L and voriconazole 0.25 mg/L for all. The overall MIC90 values were influenced by the reduced susceptibility of Candida parapsilosis isolates to echinocandins and a reduced or lack of susceptibility of Candida glabrata and Candida krusei to azoles, in particular fluconazole and itraconazole. Comparison of the LC and CC results showed good Essential Agreement (90.3% for Etest and 92.9% for Sensititre), and even higher Categorical Agreement (93.9% for Etest and 96% for Sensititre); differences were observed according to the species, method, and antifungal drug. No cross-resistance between echinocandins and triazoles was detected. Conclusions Our data confirm the different antifungal susceptibility patterns among species, and highlight the need to perform antifungal susceptibility testing of clinically relevant yeasts. With the exception of a few species (e.g. C. glabrata for azoles and C. parapsilosis for echinocandins), the findings of our study suggest that two of the most widely used commercial methods (Etest and Sensititre) provide valid and reproducible results.

Published
2011
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23. Granulomatous skin infection from Iceland.

Author

Gironi, Laura Cristina, Cammarata, Edoardo, Camaggi, Anna, Brustia, Diego, Giacalone, Angela, Andreoni, Stefano, Giorgione, Roberto, Boggio, Paolo, Astolfi, Stefano, Tiberio, Rossana, and Savoia, Paola

Published
2021
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28. Le Polmoniti: proposta di un percorso condiviso per la diagnosi microbiologica

Author

Farina Claudio, 1. 9., Andreoni, Stefano, Basaglia, Giancarlo, Capobianchi Maria rosa, Carretto, Edoardo, Del prato Bruno, Garlaschi Maria laura, Gesù Giovanni pietro, Giusti, Massimo, Langer, Martin, Lauria Francesco nicola, Manso, Esther, Marone, Piero, Nicoletti, Pierluigi, Pauri, Paola, Podestà, Alberto, Privitera, GAETANO PIERPAOLO, Sambri, Vittorio, Viale, Pierluigi, and Viganò Egidio franco

Published
2009

30. Microarray technology for yeast identification directly from positive blood cultures. A multicenter Italian experience

Author

Farina, Claudio, primary, Russello, Giuseppe, additional, Andreoni, Stefano, additional, Bonetti, Cristina, additional, Conte, Marco, additional, Fazi, Paolo, additional, Lombardi, Gianluigi, additional, Luzzaro, Francesco, additional, Manso, Esther, additional, Marone, Piero, additional, Passera, Marco, additional, Rocchetti, Andrea, additional, Sanna, Silvana, additional, and Viganò, Egidio Franco, additional

Published
2012
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33. Antifungal susceptibility of invasive yeast isolates in Italy: the GISIA3 study in critically ill patients

Author

Morace, Giulia, primary, Borghi, Elisa, additional, Iatta, Roberta, additional, Amato, Gerardino, additional, Andreoni, Stefano, additional, Brigante, Gioconda, additional, Farina, Claudio, additional, Cascio, Giuliana Lo, additional, Lombardi, Gianluigi, additional, Manso, Ester, additional, Mussap, Michele, additional, Pecile, Patrizia, additional, Rigoli, Roberto, additional, Tangorra, Elisabetta, additional, Valmarin, Maria, additional, and Montagna, Maria Teresa, additional

Published
2011
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35. Mycobacterium szulgai: articular infections case report

Author

Camaggi, Anna, primary, Ceffa, Remo, additional, Mantovani, Monia, additional, Macaluso, Paola, additional, Orlandi, Stefania, additional, Tamburelli, Sabrina, additional, Andreoni, Stefano, additional, Molinari, Gian Lorenzo, additional, Kroumova, Vesselina, additional, and Fortina, Giacomo, additional

Published
2009
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37. Italian survey on subcutaneous and deep infections by moulds

Author

Farina, Claudio, primary, Andreoni, Stefano, additional, Conte, Marco, additional, Fazii, Paolo, additional, Lombardi, Gianluigi, additional, Manso, Esther, additional, Sanna, Silvana, additional, and ‘Micosi sottocutanee e da Miceti Filamentosi’, Gruppo di Lavoro AMCLI-CoSM su, additional

Published
2009
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44. Candidurie nosocomiali: analisi dei fattori di rischio, terapia ed evoluzione micologica. Risultati di una indagine nazionale

Author

Faggi, Elisabetta, primary, Lombardi, Gianluigi, additional, Andreoni, Stefano, additional, Manso, Esther, additional, Fazii, Paolo, additional, Farina, Claudio, additional, Pini, Gabriella, additional, Brigante, Gioconda, additional, Fracchiolla, Stefania, additional, Spinelli, Mario, additional, Bonetti, Cristina, additional, D’Accardo, Anna Maria, additional, Verna, Giorgio, additional, and Anichini, Piero, additional

Published
2004
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45. In vitro fluconazole susceptibility of 1565 clinical isolates of candida species evaluated by the disk diffusion method performed using NCCLS M44-A guidelines

Author

Testore, Gian Piero, primary, Dori, Luca, additional, Buonomini, Anna Rita, additional, Schito, Gian Carlo, additional, Soro, Ornella, additional, Fortina, Giacomo, additional, Andreoni, Stefano, additional, Carlone, Nicola, additional, Tullio, Vivian, additional, and Andreoni, Massimo, additional

Published
2004
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49. Multicenter evaluation of an enzyme immunoassay (Platelia®Aspergillus) for the detection of Aspergillusantigen in serum

Author

Lombardi, Gianluigi, Farina, Claudio, Andreoni, Stefano, D'Antonio, Domenico, Faggi, Elisabetta, Manso, Esther, and Mazzoni, Aldo

Abstract

Invasive aspergillosis is a serious problem for immunocompromised patients, especially if neutropenic. The diagnosis of this infection is complicated, since clinical symptoms are often similar to those of other fungal diseases. The chance of detecting the presence of a specific antigen in the serum could confirm the suspected clinical diagnosis and, perhaps, be useful for the follow-up of the patient. The Medical Mycology Committee of the Associazione Microbiologi Clinici Italiani (AMCLI) decided to evaluate in a multicenter prospective study (from 1 November 1998 to 28 February 1999) the performance of the Platelia® AspergillusKit (Bio-Rad) for the detection of Aspergillusgalactomannan in human serum. The enrolled patients included various groups of immunosuppressed patients (mostly neutropenic). Blood samples were drawn at the time of enrollment. This decision was based upon a clinical diagnosis of probable aspergillosis (antibiotic non-responsive fever for at least 96 hours, cough, hemophthosis and positive chest X-ray). Additional blood samples were drawn on days 3, 6, 9, 12, 15 and 21. Culture and histopathologic examinations were performed according to the individual laboratory workflow. For each patient the laboratory filled a form with all the available clinical information, to create a database on which to evaluate the results of the test. During the study, 187 patients with various kinds of immunosuppression were enrolled. A total of 256 sera were tested: for 117 patients (62.6%) only the basal sample was tested, whereas for the 70 symptomatic patients (37.4%) multiple specimens (range: 1–6) were tested. The results allowed the laboratories to exclude (68.6%) or confirm (31.5%: confirmed and/or probable) the clinical diagnosis of invasive aspergillosis; 4 cases remained undetermined. Based on the results of this study, it seems that the use of this test should be limited to those patients with clinical symptoms of aspergillosis.

Published
2002
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