Search

Objective To explore the impact of mosquito collection methods, sampling Intensity and target genus on molecular xenomonitoring detection of parasites causing lymphatic filariasis. Methods We systematically searched five databases for studies that used two or more collection strategies for sampling wild mosquitoes, and employed molecular methods to assess the molecular xenomonitoring prevalence of parasites responsible for lymphatic filariasis. We performed generic inverse variance meta-analyses and explored sources of heterogeneity using subgroup analyses. We assessed methodological quality and certainty of evidence. Findings We identified 25 eligible studies, with 172 083 mosquitoes analysed. We observed significantly higher molecular xenomonitoring prevalence with collection methods that target bloodfed mosquitoes compared to methods that target unfed mosquitoes (prevalence ratio: 3.53; 95% confidence interval, CI: 1.52-8.24), but no significant difference compared with gravid collection methods (prevalence ratio: 1.54; 95% CI: 0.46-5.16). Regarding genus, we observed significantly higher molecular xenomonitoring prevalence for anopheline mosquitoes compared to culicine mosquitoes in areas where Anopheles species are the primary vector (prevalence ratio: 6.91; 95% CI: 1.73-27.52). One study provided evidence that reducing the number of sampling sites did not significantly affect molecular xenomonitoring prevalence. Evidence of differences in molecular xenomonitoring prevalence between sampling strategies was considered to be of low certainty, due partly to inherent limitations of observational studies that were not explicitly designed for these comparisons. Conclusion The choice of sampling strategy can significantly affect molecular xenomonitoring results. Further research is needed to inform the optimum strategy in light of logistical constraints and epidemiological contexts. Objectif Explorer llmpact des methodes de collecte de moustiques, de l'intensite de l'echantillonnage et du genre cible sur la detection par xenosurveillance moleculaire des parasites responsables de la filariose lymphatique. Methodes Nous avons recherche systematiquement, dans cinq bases de donnees, des etudes recourant a au moins deux strategies de collecte en vue de l'echantillonnage de moustiques a l'etat sauvage et appliquant des methodes moleculaires pour evaluer la prevalence des parasites responsables de la filariose lymphatique dans le cadre d'une xenosurveillance moleculaire. Nous avons effectue des meta-analyses generiques de variance inverse et explore les sources d'heterogeneite a l'aide d'analyses de sous-groupes. Nous avons evalue la qualite methodologique et la certitude des preuves. Resultats Nous avons identifie 25 etudes admissibles, avec 172 083 moustiques analyses. Nous avons observe une prevalence de xenosurveillance moleculaire significativement plus elevee avec les methodes de collecte ciblant les moustiques hematophages qu'avec les methodes ciblant les moustiques non hematophages (rapport de prevalence: 3,53; intervalle de confiance (IC) a 95%: 1,52-8,24), mais aucune difference significative par rapport aux methodes de collecte de femelles gravides (rapport de prevalence: 1,54; IC a 95%: 0,46-5,16). En ce qui concerne le genre, nous avons observe une prevalence de xenosurveillance moleculaire significativement plus elevee pour les moustiques anopheles que pour les moustiques culicides dans les zones oU les especes d'anopheles sont le vecteur principal (rapport de prevalence: 6,91; IC a 95%: 1,73-27,52). Une etude a mis en evidence que la reduction du nombre de sites d'echantillonnage n'avait pas d'incidence significative sur la prevalence de la xenosurveillance moleculaire. Les preuves de differences dans la prevalence de la xenosurveillance moleculaire entre les strategies d'echantillonnage ont ete considerees comme peu sures, en partie a cause des limites inherentes aux etudes d'observation qui n'ont pas ete explicitement concues pour ces comparaisons. Conclusion Le choix de la strategie d'echantillonnage peut affecter de maniere significative les resultats de la xenosurveillance moleculaire. Des recherches supplementaires sont necessaires pour determiner la strategie optimale en fonction des contraintes logistiques et des contextes epidemiologiques. Objetivo Explorar el impacto de los metodos de recoleccion de mosquitos, la intensidad del muestreo y el genero objetivo en la deteccion mediante xenomonitoreo molecular de los parasitos que causan la filariasis linfatica. Metodos Se realizaron busquedas sistematicas en cinco bases de datos de estudios que utilizaron dos o mas estrategias de recoleccion para el muestreo de mosquitos silvestres y que emplearon metodos moleculares para evaluar la prevalencia de xenomonitoreo molecular de los parasitos responsables de la filariasis linfatica. Se realizaron metanalisis genericos de varianza inversa y se exploraron las fuentes de heterogeneidad mediante analisis de subgrupos. Se evaluo la calidad de la metodologia y la certeza de las evidencias. Resultados Se identificaron 25 estudios elegibles, con 172 083 mosquitos analizados. Se observo una prevalencia significativamente mayor de xenomonitoreo molecular con metodos de recoleccion dirigidos a mosquitos hematofagos en comparacion con metodos dirigidos a mosquitos no hematofagos (razon de prevalencia: 3,53; intervalo de confianza del 95%, IC: 1,52-8,24), pero ninguna diferencia significativa en comparacion con los metodos de recoleccion de hembras gestantes (razon de prevalencia: 1,54; IC del 95%: 0,46-5,16). En cuanto al genero, se observo una prevalencia significativamente mayor de xenomonitoreo molecular para los mosquitos anofelinos en comparacion con los culicidos en zonas donde las especies de Anopheles son el vector principal (razon de prevalencia: 6,91; IC del 95%: 1,73-27,52). Un estudio aporto evidencias de que la reduccion de la cantidad de sitios de muestreo no afecto significativamente la prevalencia del xenomonitoreo molecular. Las evidencias de diferencias en la prevalencia del xenomonitoreo molecular entre las estrategias de muestreo se consideraron de baja certeza, lo que se debio en parte a las limitaciones inherentes de los estudios observacionales que no se disenaron explicitamente para estas comparaciones. Conclusion La eleccion de la estrategia de muestreo puede afectar de manera significativa a los resultados del xenomonitoreo molecular. Se requieren mas investigaciones para determinar la estrategia optima teniendo en cuenta las limitaciones logisticas y los contextos epidemiologicos., Introduction Lymphatic filariasis is a disabling and debilitating disease caused by the filarial worms Wuchereria bancrofti, Brugia malayi or B. timori that are transmitted by mosquitoes of the genera Culex, [...]

Byline: S. Galatali, T. Zengin and E. Kaya Key words: cold stress, long-term conservation, gene expression, Real-Time PCR INTRODUCTION There are more than sixty species in different regions of the [...]

Byline: Li Weiyi, Li Shaofan, Wu Xinchen, Yang Boyun and Luo Huolin Key words: Cymbidium kanran; transcriptome; flowering regulation; differentially expressed gene INTRODUCTION Cymbidium kanran (Orchidaceae) is a perennial herb, [...]

Byline: S. P. Si, J. Z. Wan, L. H. Yang, Z. Z. Wu, B. Xiong, C. H. Zhang, L. Wang and C. J. Wang Keywords Chloroplast genomes, Cupressaceae, Juniperus przewalskii, [...]

Byline: P. B. Cao Keywords: cocoa (Theobroma cacao L.), gene family, in silico, small heat shock proteins (sHSP) INTRODUCTION Heat shock protein (HSP) was firstly found in common fruit fly [...]

Byline: L. Zeng, X. Tan, S. Hu and Y. Luo Key words: Nuclear transcriptome, Rice, RNA-seq, long non-protein coding RNAs, transposable element-related genes INTRODUCTION The transcriptional landscape of nucleus extended [...]

Abstract: Kokia (Malvaceae) is a tree genus consisting only of three endangered and one extinct species endemic to the Hawaiian Islands. Morphological and molecular population analyses suggested conflicting hypotheses as [...]

Byline: N. Akhtar, A. Kiran, Sajid-ur-Rehman, M. Kausar, M. Khan, U. Saleem and T. Mahmood Key words: Gene, heat map, DNA markers, PCR, SSR. INTRODUCTION Wheat(Triticum aestivum L.) is widely [...]

Malaria is a vectorborne parasitic infection caused by Plasmodium spp. and transmitted by Anopheles mosquitoes, characterized by fever and hemolysis with chronic and fatal potential (1). Despite substantial strides toward [...]

Elizabethkingia are gram-negative, nonmotile, nonspore-forming rods that are rarely associated with human disease. E. meningoseptica is the most recognized clinically, mainly causing hospital-acquired infections, including sepsis in immunocompromised patients and [...]

The multiple genetic, environmental, and behavioral factors that play a role in the development of alcohol use disorders (AUDs) make it difficult to identify individual genes linked to these disorders. [...]

During the first decade of the new millennium, remarkable advances in technology allowed investigators in all areas of biological research to collect massive amounts of genetic data at an unprecedented [...]

ABSTRACT Previous studies have shown that hemic neoplasia in softshell clams is related to the level of p53-like mRNA in hemocytes. Traditionally, the p53-like mRNA level has been quantified using [...]