Search

Your search keyword '"Anosova, N. P."' showing total 12 results
Start Over Author "Anosova, N. P."
12 results on '"Anosova, N. P."'

8. Cholera toxin, E. coli heat-labile toxin, and non-toxic derivatives induce dendritic cell migration into the follicle-associated epithelium of Peyer's patches

Author

Anosova, N G, Chabot, S, Shreedhar, V, Borawski, J A, Dickinson, B L, and Neutra, M R

Abstract

The follicle-associated epithelium (FAE) of Peyer's patches (PPs) transports antigens and microorganisms into mucosal lymphoid tissues where they are captured by subepithelial dendritic cells (DCs). Feeding of cholera toxin (CT) induced migration of subepithelial DCs to interfollicular T-cell areas within 24 h. This study investigated short-term effects of CT, Escherichia coli heat-labile toxin, and non-toxic derivatives on DC migration. CT or CTB injected into ligated intestinal loops induced significant increase in CD11c+ DCs within the FAE within 90 min. In mice fed CT intragastrically, DC numbers in the FAE increased by 1 h, were maximal by 2 h, declined between 8 and 12 h, and were reversed by 24 h. Feeding of native LT, recombinant CTB, dibutyryl cyclic AMP, and to a lesser extent mutated CT(E29H) or mutated LT(R192G) had the same effect. Thus, both A and B subunits of enterotoxins, presumably acting through distinct signaling pathways, may promote capture of incoming antigens and pathogens by PP DCs.Mucosal Immunology (2008) 1, 59–67. doi:10.1038/mi.2007.7

Published
2008
Full Text
View/download PDF

9. Cholera toxin, E. coliheat-labile toxin, and non-toxic derivatives induce dendritic cell migration into the follicle-associated epithelium of Peyer's patches

Author

Anosova, N G, Chabot, S, Shreedhar, V, Borawski, J A, Dickinson, B L, and Neutra, M R

Abstract

The follicle-associated epithelium (FAE) of Peyer's patches (PPs) transports antigens and microorganisms into mucosal lymphoid tissues where they are captured by subepithelial dendritic cells (DCs). Feeding of cholera toxin (CT) induced migration of subepithelial DCs to interfollicular T-cell areas within 24 h. This study investigated short-term effects of CT, Escherichia coliheat-labile toxin, and non-toxic derivatives on DC migration. CT or CTB injected into ligated intestinal loops induced significant increase in CD11c+ DCs within the FAE within 90 min. In mice fed CT intragastrically, DC numbers in the FAE increased by 1 h, were maximal by 2 h, declined between 8 and 12 h, and were reversed by 24 h. Feeding of native LT, recombinant CTB, dibutyryl cyclic AMP, and to a lesser extent mutated CT(E29H) or mutated LT(R192G) had the same effect. Thus, both A and B subunits of enterotoxins, presumably acting through distinct signaling pathways, may promote capture of incoming antigens and pathogens by PP DCs.

Published
2008
Full Text
View/download PDF

10. Differential Roles of Direct and Indirect Allorecognition Pathways in the Rejection of Skin and Corneal Transplants

Author

Boisgérault, F, Liu, Ying, Anosova, N, Dana, Reza, and Benichou, Gilles A.

Subjects
cytokines, indirect allorecognition, T cells, corneal transplantation, skin graft
Abstract

Background It is generally accepted that all transplants are not rejected in the same fashion. However, the extrinsic and intrinsic factors that control the recognition and rejection of a particular allograft by the host are not well characterized. Methods We compared the mechanisms underlying the response to donor antigens by T cells activated after transplantation of fully allogeneic skin and corneal grafts in mice. Results In corneal-transplanted mice, the CD4+ T cell response was exclusively mediated by T cells recognizing minor antigens in an indirect fashion and producing low levels of IL-2. In contrast, skin grafts elicited both direct and indirect CD4+ T cell responses primarily directed to MHC antigens and characterized by high IL-2 levels. While CD8+ T cells producing γIFN were activated directly in both skin- and corneal-grafted mice, only CD8+ T cells from skin-transplanted mice mounted a cytotoxic response. Next, we investigated whether failure of corneal transplants to induce a CD4+ direct alloresponse is due to their poor immunogenicity or to the site of placement (eye). We observed that corneas transplanted under the skin as well as splenocytes transplanted in the eye were both capable of inducing direct CD4+ T cell alloreactivity. Conclusions This shows that, failure of orthotopic corneal allotransplants to elicit a CD4+ T cell direct alloresponse is associated with the combination of two factors, their low immunogenicity and the immune-privileged properties of the eye.

Published
2009
Full Text
View/download PDF

12. [Use of lornoxicam for analgesia in the early postoperative period].

Author

Nikoda VV, Maiachkin RB, Bondarenko AV, Mikhaĭlov IuE, and Anosova NP

Subjects
Adult, Aged, Anti-Inflammatory Agents, Non-Steroidal administration & dosage, Drug Therapy, Combination, Female, Humans, Infusions, Intravenous, Male, Middle Aged, Piroxicam administration & dosage, Promedol administration & dosage, Anti-Inflammatory Agents, Non-Steroidal therapeutic use, Pain, Postoperative drug therapy, Piroxicam analogs & derivatives, Piroxicam therapeutic use, Promedol therapeutic use
Abstract

Lornoxicam was used for analgesia in 64 patients on days 1-2 after extensive interventions. The drug efficiency and safety were evaluated depending on the dose and route of administration. Intravenous infusion of lornoxicam in a daily dose of 24 mg (basic therapy) did not involve the use of opioids in 35% patients and its analgesic effect was higher than that of promedol monotherapy. Combined therapy with lornoxicam and promedol allows reduction of promedol dose by 25-50% and the incidence of untoward effects by 27-44%.

Published
2001

Catalog

Books, media, physical & digital resources
See catalog results