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At the optimum temperature, which is the ideal range in which seeds germinate most efficiently, seed germination may be lower than expected under favorable conditions, and this is indicative of seed dormancy. Also, germination may be enhanced by additional and interacting factors, such as nitrate and light. However, little is known about the interplay between temperature, nitrate, and seed germination. Using seeds from 22 accessions of four Plantago species that occupy distinct pedoclimates, we applied a factorial experimental design to assess the relationship between exogenously applied nitrate (KNO3) and temperature on germination in a Petri dish experiment. The data explore the relationship between seed germination, temperatures, and seed- and maternal-source soil N content as either nitrite (NO2−), nitrate (NO3−), or ammonium (NH4+). The interpretation also considered the total N and C contents of seeds, and the soil of the maternal plant (of the test seed) sources. Significant interspecific effects of nitrate and temperature on seed germination were observed. The capacity of nitrate to enhance final germination may be diminished substantially at supra-optimum temperatures, e.g., P. lagopus germination at 15 °C was 7% lower than that seen for water-only treatment. In contrast, at sub-optimum and alternating temperatures, nitrate enhanced final germination differentially across the species tested. This suggests a shift to enhanced germination at lower temperatures in the presence of sufficient soil nitrate, facilitating seedling establishment earlier in the growing season. The seeds of some Plantago species showed increased germination as a function of nitrate and temperature, particularly those of P. lagopus. The findings indicate that species (and genotype) responses correlated with the prevailing temperature and rainfall patterns of the locality; such local adaptation would ensure that seed germination and establishment occur during a period when environmental conditions are optimal.

The challenges posed by the COVID-19 pandemic forced schools to quickly mobilize toward emergency remote teaching and learning (ERTL) practices. Teachers’ digital competences were essential in this context. This study aims to identify the ERTL practices adopted in Portugal and how they were perceived by teachers of students aged 3 to 18. Data were gathered through a questionnaire administered online during April and May 2020 to 329 teachers. ERTL was perceived as an opportunity to develop their own and their students’ digital competences. Teachers expressed a moderately positive view of the process and reported an experience of work overload. The pedagogical practices implemented by teachers during this period were diverse and differed according to gender, self-assessment of digital competence, and level of education taught. Results point to the importance of continued teacher training to achieve quality distance education.

Lithography variation presents one of the biggest challenges for photonic component optimization, especially for fabless designers. Lithography prediction models are a crucial tool for minimizing the necessary number of fabrication iterations for a device’s optimization. This paper presents one of these models specifically adapted for the contra-directional coupler structure. Through the experimental characterization of devices with a specific range of design parameters, it was possible to observe how the lithography process impacts their performance. A correction model based on effective refractive index variation and its impact on the Bragg condition of the structure was developed to predict the performance variation of a device based on the expected design variation induced by fabrication. The contra-directional couplers fabricated at CORNERSTONE foundry show a tendency to be redshifted as the gap decreases, due to an increase in waveguide width as a result of a diffraction-limited lithography process. Based on these and other findings, it was possible to correlate the design parameters to the posterior fabricated structure and ultimately predict the expected experimental response.

IntroductionWorldwide, demographic ageing is a major social, economic and health challenge. Despite the increase in life expectancy, elderly often live with multiple chronic conditions, exposing them to multiple medications. Concerns have been raised about the growing issue of inappropriate long-term usage of proton-pump inhibitors (PPI), which have been associated with adverse outcomes and increased healthcare costs. Deprescribing is a recommended intervention to reduce or withdraw medicines that might be causing harm or might no longer be of benefit. This protocol details a trial to assess the effectiveness and cost-effectiveness of a collaborative deprescribing intervention of PPI among community-dwelling elderly, involving community pharmacists and general practitioners.Methods and analysisA pragmatic, multicentre, two-arm, non-randomised controlled trial of a structured PPI collaborative deprescribing intervention in the primary care setting with a 6-month follow-up will be conducted. Patients must be 65 years old or older, live in the community and have been using PPI for more than 8 weeks. We hypothesize that the intervention will reduce the PPI usage in the intervention group compared to the control group. The primary outcome is the successful discontinuation or dose decrease of any PPI, defined as a statistically significant absolute 20% reduction in medication use between the intervention and control groups at 3- and 6-month follow-ups. An economic evaluation will be conducted alongside the trial. This study was approved by the Ethics Research Committee of Nova Medical School, NOVA University of Lisbon and by the Ethics Committee from the Local Health Unit Alto Minho, Portugal.DiscussionThis pragmatic trial will provide evidence on the effectiveness and cost-effectiveness of a patient-centred collaborative deprescribing intervention in the community setting in Portugal. It will also inform improvements for the development of future multi-faceted interventions that aim to optimise medication for the community-dwelling elderly.Clinical trial registrationISRCTN 49637686.

Photonic integrated circuits (PIC) devices are impacted by fabrication tolerances and therefore, prior knowledge of such variations could improve the PIC fabrication process and overall yield. This paper presents a method for predicting the fabrication impacts on a telecommunication optical digital to analog converter (oDAC)-based pulse amplitude modulator level four (PAM-4) transmitter as a case study where the certainty of this passive device is subjected to random variation. Our findings allow us to estimate the production yield in a fabrication scenario using the symbol error rate (SER) benchmark and this contributes to the study of the viability of oDAC PAM-4 transmitters to replace conventional electrical digital to analog converter (eDAC) PAM-4 transmitters.

MotivationSevere to profound sensorineural hearing loss interferes with a child's development at the cognitive, linguistic, academic, and social levels. Since the beginning of the pediatric auditory rehabilitation program through cochlear implantation in the Ear, Nose, and Throat (ENT) Service of the Coimbra Hospital and University Center (CHUC), Portugal, its mentors defended the early diagnosis of hearing loss followed by timely intervention, and this was considered the starting point to optimize (re)habilitation through this method. Three decades or so later, recently we conducted this study to evaluate the performance of patients implanted in the initial phase of the cochlear implantation program.ObjectivesThe study aimed to evaluate the performance of individuals with severe to profound congenital hearing loss who underwent pediatric cochlear implantation and have used the cochlear implant for at least 25 years, to analyze the beneficial effect of early intervention in improving performance results.MethodsThe study sample is composed of 31 individuals with severe to profound congenital hearing loss and no other comorbidities, divided into two groups (Group 1: age at implantation was under 3 years; Group 2: age at implantation was over 3 years). All 31 subjects were evaluated at 15, 20, and 25 years of cochlear implant (CI) use with a comprehensive set of tests. In addition, data were collected regarding the academic level of each participant. The results of both groups were compared to find out if there is an effect of age at implantation on auditory performance, and if there is an improvement in the performance with CI over time (15, 20, and 25 years of use).ResultsThe results show that there is a positive effect, with statistical significance, of early implantation on auditory performance, and telephone use. In both groups, there is an increase in performance over time, but it tends to stabilize after 20 years of CI use.Discussion and conclusionThe results obtained in this work support the importance of early intervention in patients with severe to profound hearing loss who are cochlear implant users and show that CI is an effective and reliable method in the treatment of these patients, contributing to their improved socio-educational integration, and that the benefits last over time.

Observational, cross-sectional prevalence studies are costly and time-consuming. The development of indirect methods estimating prevalence used to obtain faster, less-expensive, and more robust results would be an advantage for several healthcare applications. This study aimed to use the drug dispensing data from community pharmacies to estimate the prevalence of Type 2 Diabetes mellitus (T2DM) in the Portuguese population. A cross-sectional study was conducted using a database of dispensed medicines with an indication for Diabetes mellitus in 2018 and 2021, stratified by geographic region. The methodology was based on a sequential method of acquiring prevalence estimates obtained through exposure to medicines using the daily doses defined per thousand inhabitants per day and adjusted to the rate of adherence to therapy, prescription patterns, and concomitance of antidiabetic drugs. The estimated overall T2DM prevalence in 2018 was 13.9%, and it was 14.2% for 2021. The results show the increased consumption of antidiabetic drugs, with fixed-dose combination antidiabetics and new antidiabetics being particularly important in 2021. This work allowed for the development of a model to obtain the estimated prevalence of T2DM based on drug consumption, using a simple, fast, and robust method that is in line with the available evidence. However, with the recent expanding indications for new antidiabetics, the inclusion of further data in the model needs to be studied.

Flavescence dorée (FD) has been recorded in French vineyards since the mid-1950s; it has rapidly become a major threat to vineyard survival in different European grape-growing areas. Infection by the phytoplasma ‘Candidatus Phytoplasma vitis’ causes leaf chlorosis and disturbs primary and secondary core metabolic pathways; it can also occlude sieve plates, therefore affecting normal solute transport through the phloem. Thus, in the present study we hypothesised that the metabolomic fingerprint of the phloem sap changes in FDp-infected vines. The metabolome of the phloem sap collected by centrifugation from the bark of the stem of healthy and infected vines (cv. ‘Loureiro’) was profiled by ESI-HRMS at two snapshot time points: E-L 33 (grape berry still hard and green) and E-L 38 (grape berry at maturity). An untargeted analysis of the phloem sap enabled the identification of 476 metabolites, whereas the targeted analysis focused on eight main classes, namely acids, sugar and polyols, amino acids, phenolic acids, hormones, vitamins and N compounds, as well as on several minor classes like fatty acids, phenols or diols. Depending on the developmental stage of the vine, major differences were observed in the composition of cv. ‘Loureiro’ phloem sap in response to FD infection in terms of sugar, organic acid and amino acid content; furthermore, compounds involved in plant defense, such as salicylic acid, ABA, vitamin C and gallic acid also significantly changed. Overall, these results have contributed towards increasing knowledge about the complex interactions between vine and FD phytoplasma.

During their lifespan, grapes are exposed to conditions that can endanger plant health, including fluctuating climate and infection by pests and insects. In particular, attacks by filamentous fungi represent huge annual losses for grape farmers. Present concerns about rising antimicrobial resistance, fungicide prohibitions and shifting consumer tastes are driving the search for less harmful ways of preventing damage to grapevine by pathogenic fungi. In this study, the antagonist activity of yeasts isolated from grape berry surface microbiota was assessed by in vitro and in vivo assays on an assortment of grapevine fungal pathogens, including Botrytis cinerea and Aspergillus niger. The results show that W. anomalus, A. pullulans, C. intermedia, R. glutinis, Z. meyerae and H. takashimae were able to inhibit by 75 to 100 % mycelia growth and sporulation of the filamentous fungi in vitro. Strains of M. pulcherrima and S. bacillaris also showed 50 to 75 % efficacy in reducing mould growth. The strains were further assessed on incised leaves of 2-month-old grapevines for their ability to reduce disease incidence by the filamentous fungi. W. anomalus, A. pullulans, M. pulcherrima, C. intermedia and R. glutinis significantly reduced infection symptoms in the leaves, indicating their potential as effective biocontrol yeasts in the vineyard.

Climate changes are speeding up the maturation of grapes in numerous areas of the world, including in the Mediterranean basin, but warmer temperatures often uncouple technical and phenolic maturity, resulting in unbalanced wines. We tested the efficacy of crop forcing (CF) in delaying the maturation of cv. ‘Touriga Nacional’ vines of the Douro Region, and their impacts on plant performance, berry quality attributes and metabolome were also evaluated. In two consecutive seasons (2019 and 2020), CF was conducted 15 (CF1) and 30 (CF2) days after fruit set by hedging growing shoots to five nodes and removing summer laterals, leaves and clusters. Results showed that while CF2 delayed ripening up to 51 days till first autumn rainfall, which compromised optimal sugar ripeness, CF1 delayed the technical maturation by one month, but both treatments severely impacted the production, mainly CF1, which reduced grapevine yield up to 90 %. The effect of CF in protecting vines against drought stress was not evident, judging by the values of leaf pre-dawn water potential measured along both seasons. CF1 and CF2 resulted in berries with lower pH and higher titratable acidity than controls, while total phenolics content increased by up to 48 % in 2020. A UPLC–MS-based targeted metabolomic analysis showed that CF increased the relative abundance of key metabolites like flavan-3-ols (i.e., catechin gallate increased by up to 661 %), trihydroxylated anthocyanins (i.e., delphinidin-3-O-glucoside increased by up to 656 % after CF2) and stilbenes (resveratrol increased by up to 700 % after CF2) with potential positive impacts in wine quality.

The role of photosynthesis in fruits still challenges scientists. This is especially true in the case of mature grape berries of red varieties lined by an anthocyanin-enriched exocarp (skin) almost impermeable to gases. Although chlorophylls are degraded and replaced by carotenoids in several fruits, available evidence suggests that they may persist in red grapes at maturity. In the present study, chloroplasts were isolated from the skin of red grape berries (cv. Vinhão) to measure chlorophyll levels and the organelle proteome. The results showed that chloroplasts (and chlorophylls) are maintained in ripe berries masked by anthocyanin accumulation and that the proteome of chloroplasts from green and mature berries is distinct. Several proteins of the light reactions significantly accumulated in chloroplasts at the mature stage including those of light-harvesting complexes of photosystems I (PSI) and II (PSII), redox chain, and ATP synthase, while chloroplasts at the green stage accumulated more proteins involved in the Calvin cycle and the biosynthesis of amino acids, including precursors of secondary metabolism. Taken together, results suggest that although chloroplasts are more involved in biosynthetic reactions in green berries, at the mature stage, they may provide ATP for cell maintenance and metabolism or even O2 to feed the respiratory demand of inner tissues.

There is an increasing need for state-of-the-art Central Auditory Processing assessment for Portuguese native speakers, applicable as early as possible. As a contribution to answering this need, this paper presents a new battery for Central Auditory Processing assessment for European Portuguese applicable to children aged 5 and above, named BAPA-PE, providing information regarding test selection and development. The battery consists of six behavioral tests: Staggered Spondaic Words (SSW) for European Portuguese, Filtered Speech, Speech in Noise, Detection Interval in Noise, Duration, and Frequency Pattern. The normative data for children aged 5 to 12 are also reported. A sample was obtained of 217 subjects without ear pathology and with typical development. Each age group was composed of at least 30 children. All children were evaluated using pure tone audiometry, speech audiometry, impedance, and otoacoustic emissions. Normative scores are reported for each of the six auditory processing tests. The assessment is applicable to young children (aged 5 and 6). The statistical analyses showed significant effects in scores of Age for all tests and of Ear for several tests. The main result from the work presented, the Auditory Processing Assessment Battery—European Portuguese (BAPA-PE), is available for clinical use with normative data. This battery is a new tool for behaviorism assessment of European Portuguese speakers with suspected central auditory pathology and for monitoring the results of auditory training.

The relevance of non-Saccharomyces native yeasts for improving the complexity of wine aroma is increasingly recognised, besides their use in a range of biotechnological applications. Previously, we used a metagenomic approach to assess the effects of vineyard calcium sprays on the microbiota of grape berries cv. Vinhão and Loureiro. In this study, we aimed at assembling a catalogue of the cultivable yeasts from grape berries of these cultivars and to investigate the direct effect of calcium and other elicitors on the isolated taxa. Seventeen unique colony morphologies were identified, and the sequencing of the region spanning the rDNA internal transcribed spacers or the rDNA D1/D2 domain revealed that they are distributed into 13 different genera. Although the cultivar and vintage influenced the taxa abundance and diversity, Aureobasidium pullulans and Hanseniaspora uvarum were the most abundant species, ubiquitously populating the fruits together with Pichia terricola, Rhodotorula glutinis, Starmerella bacillaris and Vishniacozyma heimaeyensis. The rarest genera comprised Candida, Trichosporon and Zygoascus. Analysis of yeast growth in the presence of specific elicitors revealed that Pichia spp. are the most sensitive to exogenous calcium, while Sporobolomyces roseus is the most sensitive to copper exposure. Moreover, A. pullulans, H. uvarum and S. bacillaris displayed the highest sensitivity to osmotic stress. In turn, Wickerhamomyces anomalus, Metschnikowia pulcherrima, P. fermentans, P. kluyveri and P. kudriavzevii showed mild resistance to ethanol, unlike the remaining non-Saccharomyces yeasts. Correlation networks expressing the overall yeast performance showed that H. uvarum and S. bacillaris share the strongest positive interspecies correlation, followed by the pairs P. fermentans–P. kluyveri, B. albus–R. glutinis and A. pullulans–V. heimaeyensis. The strongest negative correlations were found for the pairs P. kluyveri–S. roseus, P. terricola–W. anomalus, P. kudriavzevii–V. heimayensis, P. kluyveri–V. heimayensis, and A. pullulans–P. kluyveri. This study exposed microbial niches with a predictably common response to abiotic stress.

Photonic Integrated Circuits (PICs) are taking a major role in the telecommunications and datacenter markets. The increased complexity of coexisting and fast evolving standards for Passive Optical Networks (PONs) suggests the introduction of PICs will be the next step in PON related optoelectronics. The PICs ecosystem has greatly matured in the past years, becoming a solution that can cope with the requirements of industry and academia, and presenting the flexibility of combining multiple platforms available towards viable commercial solutions. In this review, the evolution of PONs and PICs is presented, with a focus on the optoelectronic integration of PICs for PONs and coherent PONs. To demonstrate the potential of PICs and their combination with electronics, a quasi-coherent receiver based on co-hosted PIC and Application Specific Integrated Circuit (ASIC) is presented and characterized.

Vineyard calcium (Ca) sprays have been increasingly used by grape growers to improve fruit firmness and thus maintain quality, particularly in periods of heavy rains and hail. The observation that Ca visibly modified berry size, texture, and color in the most prominent white cultivar of the DOC region ‘Vinhos Verdes’, cultivar (cv.) Loureiro, led us to hypothesize that Ca induced metabolic rearrangements that resulted in a substantial delay in fruit maturation. Targeted metabolomics by ultra-performance liquid chromatography coupled to mass spectrometry and directed transcriptomics were thus combined to characterize the metabolic and transcriptional profiles of cv. Loureiro berries that, together with firmness, °Brix, and fruit weight measurements, allowed to obtain an integrated picture of the biochemical and structural effects of Ca in this cultivar. Results showed that exogenous Ca decreased amino acid levels in ripe berries while upregulating PAL1 expression, and stimulated the accumulation of caftaric, coutaric, and fertaric acids. An increase in the levels of specific stilbenoids, namely E-piceid and E-ω-viniferin, was observed, which correlated with the upregulation of STS expression. Trace amounts of anthocyanins were detected in berries of this white cultivar, but Ca treatment further inhibited their accumulation. The increased berry flavonol content upon Ca treatment confirmed that Ca delays the maturation process, which was further supported by an increase in fruit firmness and decrease in weight and °Brix at harvest. This newly reported effect may be specific to white cultivars, a topic that deserves further investigation.

In recent years, the Open Educational Resources (OER) and Open Access (OA) movements have been essential in creating opportunities in all scholarly activities, within the context of higher education. The main purpose of this research was to understand how perceptions and practices of faculty towards OER are related to their perceptions and practices towards OA. It is an exploratory and descriptive study, with a mixed methods approach, undertaken in Portugal. Results indicate that, although faculty already show some degree of knowledge and use of OER and OA in their teaching and research practices, there is still a general lack of knowledge in both fields. However, the convergence of perceptions regarding both fields provide evidence on the possibility of a common approach to both fields in faculty’s educational practices, with the purpose of opening up their educational and scientific resources, thus reinforcing the principles of transparency, collaboration and openness to knowledge.

BackgroundSeveral studies have investigated the acoustic effects of diagnosed anxiety and depression. Anxiety and depression are not characteristics of the typical aging process, but minimal or mild symptoms can appear and evolve with age. However, the knowledge about the association between speech and anxiety or depression is scarce for minimal/mild symptoms, typical of healthy aging. As longevity and aging are still a new phenomenon worldwide, posing also several clinical challenges, it is important to improve our understanding of non-severe mood symptoms' impact on acoustic features across lifetime. The purpose of this study was to determine if variations in acoustic measures of voice are associated with non-severe anxiety or depression symptoms in adult population across lifetime.MethodsTwo different speech tasks (reading vowels in disyllabic words and describing a picture) were produced by 112 individuals aged 35-97. To assess anxiety and depression symptoms, the Hospital Anxiety Depression Scale (HADS) was used. The association between the segmental and suprasegmental acoustic parameters and HADS scores were analyzed using the linear multiple regression technique.ResultsThe number of participants with presence of anxiety or depression symptoms is low (>7: 26.8% and 10.7%, respectively) and non-severe (HADS-A: 5.4 ± 2.9 and HADS-D: 4.2 ± 2.7, respectively). Adults with higher anxiety symptoms did not present significant relationships associated with the acoustic parameters studied. Adults with increased depressive symptoms presented higher vowel duration, longer total pause duration and short total speech duration. Finally, age presented a positive and significant effect only for depressive symptoms, showing that older participants tend to have more depressive symptoms.ConclusionsNon-severe depression symptoms can be related to some acoustic parameters and age. Depression symptoms can be explained by acoustic parameters even among individuals without severe symptom levels.

European Portuguese (EP) is characterized by a large number of nasals encompassing five phonemic nasal vowels. One notable characteristic of these sounds is their dynamic nature, involving both oral and nasal gestures, which makes their study and characterization challenging. The study of nasal vowels, in particular, has been addressed using a wide range of technologies: early descriptions were based on acoustics and nasalance, later expanded with articulatory data obtained from EMA and real-time magnetic resonance (RT-MRI). While providing important results, these studies were limited by the discrete nature of the EMA-pellets, providing only a small grasp of the vocal tract; by the small time resolution of the MRI data; and by the small number of speakers. To tackle these limitations, and to take advantage of recent advances in RT-MRI allowing 50 fps, novel articulatory data has been acquired for 11 EP speakers. The work presented here explores the capabilities of recently proposed data-driven approaches to model articulatory data extracted from RT-MRI to assess their suitability for investigating the dynamic characteristics of nasal vowels. To this end, we explore vocal tract configurations over time, along with the coordination of velum and lip aperture in oral and nasal bilabial contexts for nasal vowels and oral congeners. Overall, the results show that both generalized additive mixed models (GAMMs) and functional linear mixed models (FLMMs) provide an elegant approach to tackle the data from multiple speakers. More specifically, we found oro-pharyngeal differences in the tongue configurations for low and mid nasal vowels: vowel track aperture was larger in the pharyngeal and smaller in the palatal region for the three non-high nasal vowels, providing evidence of a raised and more advanced tongue position of the nasal vowels. Even though this work is aimed at exploring the applicability of the methods, the outcomes already highlight interesting data for the dynamic characterization of EP nasal vowels.

A pandemia de Covid-19 teve impactos profundos no ensino superior. Em Portugal, afetou cerca de 350000 estudantes, envolvidos num processo de transição de emergência para práticas de ensino remoto. Consideramos fundamental identificar práticas adotadas pelas instituições de ensino superior nesse contexto, assim como a forma como têm sido reajustadas ao longo do tempo, e como o processo foi percecionado, quer por professores, quer por estudantes. Para tal, foi aplicado um questionário online dirigido a professores e estudantes do ensino superior presencial entre os dias 22 de abril e 12 de maio de 2020. Os resultados apontam para a adoção de uma pluralidade de estratégias de ensino, com destaque para práticas síncronas, e revistas ao longo do processo. Apesar de aspetos sensíveis como a avaliação, o envolvimento dos estudantes, as atividades práticas ou a gestão do tempo, são reconhecidas potencialidades e limitações ao processo.

The success of seed-based conservation and restoration efforts using native plant species is largely determined by ensuring two key life history transitions are accommodated. These are from “seed to germinated seed” and “germinated seed to established seedling.” In turn, optimization of these life history transitions is determined by a “genetic × environmental” interaction and later largely characterized by localized climatic (abiotic) conditions. It is these environmental stress factors that can act as natural selection agents for specific plant–trait combinations, or phenotypes. In turn, such adaptation may also limit a species range. To test the relationship between these two early plant life history stage transitions, “seed to germinated seed” and “germinated seed to established seedling,” the attributes were characterized for two species of Plantago that occupy contrasting environments and since these species have potential for native seed-based habit restoration and conservation. The species were Plantago coronopus (L.), localized at lower and drier altitudes, and Plantago lanceolata (L.), characterized as occupying higher and wetter altitudinal clines. Seeds were collected from 20 accessions of six natural populations spanning four European countries for both P. lanceolata and P. coronopus. Seed germination (G) and seedling establishment (S) data were determined at six temperatures (T) and six water potentials (Ψ), and the data obtained were analyzed using a generalized linear model (GLM). The results indicate that P. coronopus has adapted physiologically to its high-altitude conditions such that seed germination and seedling establishment may be more readily achieved in this cooler environment where water is less limiting. In contrast, the lower θT of P. lanceolata better facilitates more efficient seed germination and seedling establishment in drier and warmer clines of lower altitude. In addition to establishing a genotypic (species) underpin for seed and seedling trait differences observed, the insights gained may also be exploited to best deploy each species in situ for seed-based conservation and restoration efforts.

Flavescence dorée (FD), caused by the phytoplasma Candidatus Phytoplasma vitis, is a major threat to vineyard survival in different European grape-growing areas. It has been recorded in French vineyards since the mid-1950s, and rapidly spread to other countries. In Portugal, the phytoplasma was first detected in the DOC region of ‘Vinhos Verdes’ in 2006, and reached the central region of the country in 2009. The infection causes strong accumulation of carbohydrates and phenolics in the mesophyll cells and a simultaneous decrease of chlorophylls, events accompanied by a down regulation of genes and proteins involved in the dark and light-dependent reactions and stabilization of the photosystem II (PSII). In the present study, to better elucidate the basis of the leaf chlorosis in infected grapevine cv. Loureiro, we studied the isoprenoid transcript–metabolite correlation in leaves from healthy and FD-infected vines. Specifically, targeted metabolome revealed that twenty-one compounds (out of thirty-two), including chlorophylls, carotenoids, quinones and tocopherols, were reduced in response to FD-infection. Thereafter, and consistently with the biochemical data, qPCR analysis highlighted a severe FD-mediated repression in key genes involved in isoprenoid biosynthetic pathways. A more diverse set of changes, on the contrary, was observed in the case of ABA metabolism. Principal component analysis (PCA) of all identified metabolites clearly separated healthy from FD-infected vines, therefore confirming that the infection strongly alters the biosynthesis of grapevine isoprenoids; additionally, forty-four genes and metabolites were identified as the components mostly explaining the variance between healthy and infected samples. Finally, transcript–metabolite network correlation analyses were exploited to display the main hubs of the infection process, which highlighted a strong role of VvCHLG, VvVTE and VvZEP genes and the chlorophylls intermediates aminolevulunic acid and porphobilinogen in response to FD infection. Overall, results indicated that the FD infection impairs the synthesis of isoprenoids, through the repression of key genes involved in the biosynthesis of chlorophylls, carotenoids, quinones and tocopherols.

The introduction of irrigation in vineyards of the Mediterranean basin is a matter of debate, in particular in those of the Douro Demarcated Region (DDR), due to the limited number of available studies. Here, we aimed to perform a robust analysis in three consecutive vintages (2018, 2019, and 2020) on the impact of deficit irrigation on the yield, berry quality traits, and metabolome of cv. ‘Touriga Nacional’. Results showed that in the peaks of extreme drought, irrigation at 30% crop evapotranspiration (ETc) (R30) was able to prevent a decay of up to 0.4 MPa of leaf predawn water potential (ΨPd), but irrigation at 70% ETc (R70) did not translate into additional protection against drought stress. Following three seasons of irrigation, the yield was significantly improved in vines irrigated at R30, whereas irrigation at R70 positively affected the yield only in the 2020 season. Berry quality traits at harvest were not significantly changed by irrigation, except for Total Soluble Solids (TSS) in 2018. A UPLC–MS-based targeted metabolomic analysis identified eight classes of compounds, amino acids, phenolic acids, stilbenoid DP1, stilbenoid DP2, flavonols, flavan-3-ols, di-OH- and tri-OH anthocyanins, and showed that anthocyanins and phenolic acids did not change significantly with irrigation. The present study showed that deficit irrigation partially mitigated the severe summer water deficit conditions in the DDR but did not significantly change key metabolites.

For aging speech, there is limited knowledge regarding the articulatory adjustments underlying the acoustic findings observed in previous studies. In order to investigate the age-related articulatory differences in European Portuguese (EP) vowels, the present study analyzes the tongue configuration of the nine EP oral vowels (isolated context and pseudoword context) produced by 10 female speakers of two different age groups (young and old). From the tongue contours automatically segmented from the US images and manually revised, the parameters (tongue height and tongue advancement) were extracted. The results suggest that the tongue tends to be higher and more advanced for the older females compared to the younger ones for almost all vowels. Thus, the vowel articulatory space tends to be higher, advanced, and bigger with age. For older females, unlike younger females that presented a sharp reduction in the articulatory vowel space in disyllabic sequences, the vowel space tends to be more advanced for isolated vowels compared with vowels produced in disyllabic sequences. This study extends our pilot research by reporting articulatory data from more speakers based on an improved automatic method of tongue contours tracing, and it performs an inter-speaker comparison through the application of a novel normalization procedure.

Speech is our most natural and efficient form of communication and offers a strong potential to improve how we interact with machines. However, speech communication can sometimes be limited by environmental (e.g., ambient noise), contextual (e.g., need for privacy), or health conditions (e.g., laryngectomy), preventing the consideration of audible speech. In this regard, silent speech interfaces (SSI) have been proposed as an alternative, considering technologies that do not require the production of acoustic signals (e.g., electromyography and video). Unfortunately, despite their plentitude, many still face limitations regarding their everyday use, e.g., being intrusive, non-portable, or raising technical (e.g., lighting conditions for video) or privacy concerns. In line with this necessity, this article explores the consideration of contactless continuous-wave radar to assess its potential for SSI development. A corpus of 13 European Portuguese words was acquired for four speakers and three of them enrolled in a second acquisition session, three months later. Regarding the speaker-dependent models, trained and tested with data from each speaker while using 5-fold cross-validation, average accuracies of 84.50% and 88.00% were respectively obtained from Bagging (BAG) and Linear Regression (LR) classifiers, respectively. Additionally, recognition accuracies of 81.79% and 81.80% were also, respectively, achieved for the session and speaker-independent experiments, establishing promising grounds for further exploring this technology towards silent speech recognition.

Our homes are becoming increasingly sensorized and smarter. However, they are also becoming increasingly complex, making accessing them and their advantages difficult. Assistants have the potential for improving the accessibility of smart homes, by providing everyone with an integrated, natural, and multimodal way of interacting with the home’s ecosystem. To demonstrate this potential and contribute to more environmentally friendly homes, in the scope of the project Smart Green Homes, a home assistant highly integrated with an ICT (Information and communications technology) home infrastructure was developed, deployed in a demonstrator, and evaluated by seventy users. The users’ global impression of our home assistant is in general positive, with 61% of the participants rating it as good or excellent overall and 51% being likely or very likely to recommend it to others. Moreover, most think that the assistant enhances interaction with the smart home’s multiple devices and is easy to use by everyone. These results show that a home assistant providing an integrated view of a smart home, through natural, multimodal, and adaptive interaction, is a suitable solution for enhancing the accessibility of smart homes and thus contributing to a better living ambient for all of their inhabitants.

Emergency remote teaching and learning (ERTL) represent a critical and challenging time for teachers globally. To understand how Portuguese teachers interpreted their ERTL experiences of the first lockdown, we started by asking: What perspectives do teachers have about ERTL? Answering three open questions made available online between April and May of 2020, a sample of 305 preschool, basic, and secondary school teachers expressed their perspectives on (i) difficulties throughout ERTL; (ii) students’ constraints in participating in ERTL; (iii) the potential and benefits resulting from this exceptional period. Data were analyzed using content analysis. While Portuguese teachers perceived ERTL with concern, a majority also saw it as an opportunity. Workload, work conditions, and time management were the most frequently mentioned difficulties. Regarding students’ constraints, teachers emphasized participation, the role of parents, lack of contact, and autonomy. Despite the constraints and difficulties, teachers highlighted gains such as the development of digital competencies and the opportunity to transform teaching and learning. Only 18 teachers suggested there were no positive outcomes from this experience. Since this is an exploratory study, further studies are needed to triangulate the findings.

Background: Antibiotic resistance is a worldwide public health problem, leading to longer hospital stays, raising medical costs and mortality levels. As physicians’ attitudes are key factors to antibiotic prescribing, this study sought to explore their differences between primary care and hospital settings. Methods: A survey was conducted between September 2011 and February 2012 in the center region of Portugal in the form of a questionnaire to compare hospital (n = 154) and primary care (n = 421) physicians’ attitudes and knowledge regarding antibiotic prescribing. Results: More than 70% of the attitudes were statistically different (p < 0.05) between hospital physicians (HPs) and primary care physicians (PCPs). When compared to PCPs, HPs showed higher agreement with antibiotic resistances being a public health problem and ascribed more importance to microbiological tests and to the influence of prescription on the development of resistances. On the other hand, PCPs tended to agree more regarding the negative impact of self-medication with antibiotics dispensed without medical prescription and the need for rapid diagnostic tests. Seven out of nine sources of knowledge’s usefulness were statistically different between both settings, with HPs considering most of the knowledge sources to be more useful than PCPs. Conclusions: Besides the efforts made to improve both antibiotic prescribing and use, there are differences in the opinions between physicians working in different settings that might impact the quality of antibiotic prescribing. In the future, these differences must be considered to develop more appropriate interventions.

Technologies, such as real-time magnetic resonance (RT-MRI), can provide valuable information to evolve our understanding of the static and dynamic aspects of speech by contributing to the determination of which articulators are essential (critical) in producing specific sounds and how (gestures). While a visual analysis and comparison of imaging data or vocal tract profiles can already provide relevant findings, the sheer amount of available data demands and can strongly profit from unsupervised data-driven approaches. Recent work, in this regard, has asserted the possibility of determining critical articulators from RT-MRI data by considering a representation of vocal tract configurations based on landmarks placed on the tongue, lips, and velum, yielding meaningful results for European Portuguese (EP). Advancing this previous work to obtain a characterization of EP sounds grounded on Articulatory Phonology, important to explore critical gestures and advance, for example, articulatory speech synthesis, entails the consideration of a novel set of tract variables. To this end, this article explores critical variable determination considering a vocal tract representation aligned with Articulatory Phonology and the Task Dynamics framework. The overall results, obtained considering data for three EP speakers, show the applicability of this approach and are consistent with existing descriptions of EP sounds.

The development of photonic integrated circuits (PIC) for access network applications, such as passive optical networks (PON), constitutes a very attractive ecosystem due to PON’s potential mass market. The implementation of PIC solutions in this context is expected to facilitate the possibility of increasing the complexity and functionalities of devices at a potentially lower cost. We present a review addressing the prominent access network market requirements and the main restrictions stemming from its specific field of application. Higher focus is given to PON devices for the optical network unit (ONU) and the implications of designing a device ready for market by discussing its various perspectives in terms of technology and cost. The discussed PIC solutions/approaches in this paper are mainly based on indium phosphide (InP) technology, due to its monolithic integration capabilities. A comprehensive set of guidelines considering the current technology limitations, benefits, and processes are presented. Additionally, key current approaches and efforts are analyzed for PON next generations, such as next-generation PON 2 (NGPON2) and high-speed PON (HSP).

The main goal of this Special Issue is to present the latest advances in research and novel applications of speech and language technologies based on the works presented at the IberSPEECH edition held in Barcelona in 2018, paying special attention to those focused on Iberian languages. IberSPEECH is the international conference of the Special Interest Group on Iberian Languages (SIG-IL) of the International Speech Communication Association (ISCA) and of the Spanish Thematic Network on Speech Technologies (Red Temática en Tecnologías del Habla, or RTTH for short). Several researchers were invited to extend their contributions presented at IberSPEECH2018 due to their interest and quality. As a result, this Special Issue is composed of 13 papers that cover different topics of investigation related to perception, speech analysis and enhancement, speaker verification and identification, speech production and synthesis, natural language processing, together with several applications and evaluation challenges.

Massive Open Online Courses (MOOCs) are a new disruptive development in higher education that combines openness and scalability in a most powerful way. They have the potential to widen participation in higher education. Thus, they contribute to social inclusion, the dissemination of knowledge and pedagogical innovation and also the internationalization of higher education institutions. However, one of the critical elements for a massive open language learning experience to be successful is to empower learners and to facilitate networked learning experiences. In fact, MOOCs are designed for an undefined number of participants, thus serving a high heterogeneity of profiles, with diverse learning styles and prior knowledge, and also contexts of participation and diversity of online platforms. Personalization can play a key role in this process. The iMOOC pedagogical model introduced the notion of diversity to MOOC design, allowing for a clear differentiation of learning paths and also virtual environments. In this article, the authors present a proposal based on the iMOOC approach for a new framework for personalizing and adapting MOOCs designed in a collaborative, networked pedagogical approach by identifying each participant's competence profile and prior knowledge, as well as the respective mobile communication device used to generate matching personalized learning. This article also shows the results obtained in a laboratory environment after an experiment has been performed with a prototype of the framework. It can be observed that creating personalized learning paths is possible and the next step is to test this framework with real experimental groups.

OBJECTIVO:avaliar a influencia do gênero no desenvolvimento da linguagem de crianças com surdez severa a profunda neurossensorial, após implante coclear.MÉTODOS:foram estudadas 30 crianças, 12 do gênero feminino e 18 do masculino, entre os 8 anos e 1 mês e os 10 anos com surdez severa a profunda neurossensorial bilateral congênita com implante coclear. Avaliaram-se as estruturas linguísticas semântica, morfossintaxe e fonologia. Utilizou-se o instrumento de avaliação: Grelha de Observação da Linguagem - nível escolar.RESULTADOS:a idade auditiva média foi de 72 meses no gênero feminino e 72.7 meses no masculino. As pontuações obtidas nos três níveis linguisticos foram estatisticamente semelhantes, quando comparados os gêneros. Nas provas de cada estrutura linguística também não se verificaram diferenças estatisticamente significantes.CONCLUSÕES:o gênero não influenciou o desenvolvimento da linguagem oral nas crianças utilizadoras de implante coclear, nas três estruturas linguísticas estudadas.

Vinte e cinco anos de uma organização entrecruzam-se, necessariamente, com um elevado número de pessoas cuja dedicação e contributo são indissociáveis da construção da sua identidade única. É sobre esta identidade que 5 pares de mão escrevem este testemunho despretensioso e enviesado pelas suas próprias histórias de vida e pela forma como viveram cada momento na construção. Ao contrário da geração da evidência a que o Centro de Estudos e Avaliação em Saúde (CEFAR) se tem dedicado, este manuscrito sofrerá de limitações inultrapassáveis por substituir dados por experiências e ciência por emoções. Contudo, tal como as publicações que encaderna, foi construído com o propósito de testemunhar, neste caso dimensões que geralmente escapam ao olhar frio e desapaixonado com que se constrói ciência. É também um legado incompleto e inacabado, como a própria ciência, que avança e se transforma como uma construção de homens e mulheres ao serviço de uma missão que ultrapassa a individualidade.

Technologies, such as smart sensors, actuators, and other kinds of devices, are often installed in our environments (e.g., our Homes) and available to integrate our daily lives. Despite their installation being motivated by the pursuit of automation and increased efficiency, making these environments usable, acceptable and enjoyable in a sustainable, energy efficient way is not only a matter of automation. Tackling these goals is a complex task demanding the combination of different perspectives including building and urban Architecture, Ubiquitous Computing and Human-Computer Interaction (HCI) to provide occupants with the means to shape these environments to their needs. Interaction is of paramount relevance in the creation of adequate relations of users with their environments, but it cannot be seen independently from the ubiquitous sensing and computing or the environment’s architecture. In this regard, there are several challenges to HCI, particularly in how to integrate this multidisciplinary effort. Although there are several solutions to address some of these challenges, the complexity and dynamic nature of the smart environments and the diversity of technologies involved still present many challenges, particularly for its development. In general, the development is complex, and it is hard to create a dynamic environment providing versatile and adaptive forms of interaction. To participate in the multidisciplinary effort, the development of interaction must be supported by tools capable of facilitating co-design by multidisciplinary teams. In this article, we address the development of interaction for complex smart environments and propose the AM4I architecture and framework, a novel modular approach to design and develop adaptive multiplatform multilingual multi-device multimodal interactive systems. The potential of the framework is demonstrated by proof-of-concept applications in two different smart environment contexts, non-residential buildings and smart homes.

Backgound and Objectives: Considering the role of antibiotic prescription on the development of antimicrobial resistances, the aim of this study was to assess the reproducibility and the internal consistence of one questionnaire in web and paper versions, designed to study physicians’ knowledge about antibiotic use. Methods: It was developed an observational pilot study for questionnaire evaluation (test-retest) in the Grouping of Primary Care Facilities Grande Porto – II Gondomar, Portugal, which included 81 General Practitioners. The reproducibility and internal consistence were calculated, respectively, by Intraclass Correlation Coeffi cient and Cronbach’s alpha. Results: Response rate was higher for web-based than paper version questionnaire [57% (paper); 44% (online)]. Internal consistence (Cronbach’s alpha) of questionnaire, were satisfactory for both versions of the questionnaire [0,899 (online version); 0,770 (paper version)]. The results of Intraclass Correlation Coeffi cients were close for both versions of the questionnaire. Conclusions: Despite the results of both versions being close, the use of web-based questionnaires demands a deep analysis of its intrinsic limitations as a tool of data collection, namely because of the lower response rates or nonresponse bias.

En este trabajo se analizan las consecuencias, desde el punto de vista organizativo, de la adopción generalizada de prácticas educativas abiertas en las instituciones de educación superior. Partiendo del análisis crítico del modelo de Wiley Hilton, en el artículo se exploran las implicaciones que, a la educación abierta, aportan los modelos de negocios y la gobernanza institucional. Por último, se propone un nuevo tipo de modelo de organización de tipo deconstructivo, lo que permite una mayor flexibilidad institucional a los desafíos externos y nos hace más preparados para una cultura de innovación abierta.

In this text, dedicated to the study of the relations between the human rights and globalization, we try to conceptualize the emergence of the problematic of human rights and its historic development. Besides a discussion about the foundation and nature of human rights, we analyse the complex relation between the proclamation of their universality and their instrumentalization in practice

In the present text we identify some of the main properties of a set of propositions about the sociological relations that can be established between the democratic processes and citizenship. We specifically highlight the properties that can be conceptualized in the frame of a liberal model and the ones that can be red in the light of a republican model. Given the description that is done, we try to sketch some of the main obstacles (that are at the same time challenges) to the development of democracy and citizenship

As a consequence of changes in apparatus structure and Code of Points, gymnasts are using the straight bodyposition more and more in Uneven Bars routines. The purpose of the present study was to analyze the influence of “in bar”elements on body position during Uneven Bars routines. Observational methodology was used to construct and validate twoobservation categories comprising eleven variables considered as indicators of the external load in uneven bars. 83 unevenbars routines were analyzed from world championship and Olympic Games finals between 1989 and 2004. The principalresults we observed were significant increases in the execution of “in bar” elements, with and without longitudinal rotations,as well as in respective difficulty. Straight body position elements increased from 6.45 to 9.71 up to 2001 and decreased to7.88 by 2004.Close body position elements ranged from 8.15 to 8.21 until 2001 and then increased to 10.94 by 2004. Wemay conclude that compulsory use of “in bar” elements and their execution beyond Code requirements contradicted thetrends towards increased use of straight body positions.

MOOCs are a recent phenomenon, although given its impact, have been subject to a large debate. Several questions have been raised by researchers and educators alike as regarding its sustainability both economical and as an efficient mode of education provision. In this paper we contribute to this discussion by presenting a case study, a Portuguese MOOC about lived experiences in climate change which piloted the iMOOC pedagogical model developed at Universidade Aberta. The iMOOC is an hybrid model which incorporates elements from existing MOOCs but adds other features drawn from UAb's experience with online learning and aim at better integrate in the larger context of the institutional pedagogical culture. The iMOOC implied also an integration of platforms - Moodle and Elgg. The course had more than one thousand participants, and it was the largest MOOC course on Portuguese language delivered so far. We discuss the effort required to design and deliver the course, the technological solution developed, and the results obtained. We registered a moderate effort to create and run the course, ensured by internal staff from the University. The technological solution was a success, an integrated architecture combining well-established, well-tested open software. The completion rate was 3.3%, but the high success of this innovative learning experience is demonstrated by the active involvement of participants, almost 50% of the ones that followed the course until the end, and the satisfaction survey results, with 90% of approval. Lessons learned from this experience and future research on the field are also discussed. Keywords: Massive open online course, iMOOC, pedagogical model, learning effectiveness, completion rate, cost analysis.

Introdução: As crianças com surdez neurossensorial severa a profunda podem adquirir vocabulário e estruturas sintácticas para comunicarem pela linguagem oral, após implante coclear. Objectivo: Identificar as capacidades linguísticas em crianças, com implante coclear. Material e Métodos: Estudou-se a linguagem oral em 18 crianças, entre nove e 10 anos, com surdez neurossensorial profunda bilateral congénita, com implante coclear, avaliadas com a Grelha de Observação da Linguagem-Nível Escolar. As pontuações obtidas foram comparadas com as das crianças normo-ouvintes com igual idade auditiva. Resultados: As pontuações nas estruturas linguísticas estudadas, crianças implantadas vs. padrão das normo-ouvintes foram: fonologia 29,44 ± 8,4 vs. 29,68 ± 5,90, p = 0,91; semântica 18,55 ± 8,89 vs. 19,20 ± 4,85, p = 0,76; morfossintaxe 21,89 ± 12,85 vs. 26,35 ± 10,36, p = 0,159. Nas provas da estrutura semântica, não se registaram diferenças estatisticamente significativas. Na morfossintaxe, a diferença foi estatisticamente significativa na derivação das palavras, 2,83 ± 2,81 vs. 4,65 ± 1,64, p = 0,014. Na fonologia, verificou-se diferença significativa, na prova de discriminação de pseudopalavras, 6,6 ± 2,8 vs. 8,37 ± 2,32, p = 0,023. Na segmentação silábica, as crianças implantadas tiveram uma pontuação significativamente superior ao padrão 8,56 ± 1,6 vs. 5,9 ± 1,58, p < 0,001. Discussão: A semelhança das pontuações obtidas pelas crianças com implante coclear em relação ao padrão nas componentes linguísticas estudadas, confirma que o implante coclear promove o desenvolvimento da linguagem verbal oral nas crianças com surdez congénita. Conclusão: As crianças implantadas obtiveram ganhos de linguagem similares às normo-ouvintes com igual idade auditiva.

Novos equipamentos como 'smartphones' ou 'tablets' têm revolucionado a interacção do ser humano com a tecnologia, proporcionando novos desafios e oportunidades. Estes novos dispositivos são multimodais por natureza. De entre as várias modalidades, são particularmente interessantes as relacionadas com a interacção por voz e texto. Para que estas formas de interação possam ser usadas entre sistemas e utilizadores humanos, é essencial a existência de módulos capazes de traduzir as informações internas das aplicações em frases ou textos, para visualização no ecrã ou para serem sintetizados de forma a serem ouvidos. É, também, essencial que estes módulos possam gerar frases e textos nas línguas nativas dos utilizadores; que o processo de desenvolvimento não implique grandes conhecimentos e recursos, incluindo tempo de desenvolvimento; e o resultado da geração apresente a variabilidade necessária. O objectivo principal é o de propor, implementar e avaliar um método de conversão de Dados-para-português passível de ser desenvolvido com um mínimo de tempo e conhecimentos, mas sem comprometer a indispensável variabilidade e qualidade do que é gerado. O sistema apresentado, desenvolvido para um cenário de assistência à toma de medicamentos, destina-se a criar descrições, em linguagem natural, de informação sobre medicação a tomar. Motivados por resultados recentes, optou-se por uma abordagem baseada em tradução automática, com os modelos treinados num pequeno corpus paralelo. Para isso, foi criado um novo corpus que, depois de validado, foi utilizado no desenvolvimento do sistema. Foram criadas duas variantes do sistema: uma orientada à tradução baseada em sintagmas e outra fazendo uso de informação sintáctica. Foram realizadas avaliações utilizando métricas automáticas -- BLEU e Meteor -- bem como avaliações por humanos. Os resultados do sistema orientado a sintagmas foram francamente superiores aos do seu concorrente, obtendo uma média por avaliador humano de 60% de frases consideradas inteligíveis, contra 46% do seu congénere, o que pode considerar-se um bom resultado tendo em conta a dimensão do corpus.

Automatic translation is usually related to conversion between human languages. Nevertheless, in human-machine interaction scenarios new forms of translation emerged. This work presents two examples. First, from the area of Natural Language Generation, is presented a data-to-text system, where data stored in a database regarding a medication plan is translated to Portuguese. As second example, is presented a system addressing the transmission of information from humans to computers, showing that automatic translation can be useful in the development of systems that use voice commands for interaction and having multilingualism as a requirement. The examples presented, part of our recent work, demonstrate the increase of application areas for automatic translation, area that received many and valuable contributions from Belinda Maia.

Nasality is a very important characteristic of several languages, European Portuguese being one of them. This paper addresses the challenge of nasality detection in surface electromyography (EMG) based speech interfaces. We explore the existence of useful information about the velum movement and also assess if muscles deeper down in the face and neck region can be measured using surface electrodes, and the best electrode location to do so. The procedure we adopted uses Real-Time Magnetic Resonance Imaging (RT-MRI), collected from a set of speakers, providing a method to interpret EMG data. By ensuring compatible data recording conditions, and proper time alignment between the EMG and the RT-MRI data, we are able to accurately estimate the time when the velum moves and the type of movement when a nasal vowel occurs. The combination of these two sources revealed interesting and distinct characteristics in the EMG signal when a nasal vowel is uttered, which motivated a classification experiment. Overall results of this experiment provide evidence that it is possible to detect velum movement using sensors positioned below the ear, between mastoid process and the mandible, in the upper neck region. In a frame-based classification scenario, error rates as low as 32.5% for all speakers and 23.4% for the best speaker have been achieved, for nasal vowel detection. This outcome stands as an encouraging result, fostering the grounds for deeper exploration of the proposed approach as a promising route to the development of an EMG-based speech interface for languages with strong nasal characteristics.