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This work introduces a ground-breaking electrochemical sensor for selectively detecting arsenic, a toxic contaminant, in water. The sensor merges the high sensitivity of stripping voltammetry with the powerful data analysis of chemometrics, achieving exceptional detection limits. By optimizing deposition potential, time, and scan rate, the analytical response is significantly enhanced. A chemometric approach based on principal component analysis (PCA) is implemented to address potential interference from other ions. This technique effectively isolates arsenic signals from background noise and interfering species, leading to superior selectivity and accurate quantification. The proposed method boasts a detection limit of 0.4 nM and a sensitivity of 176.34 µA µM−1 for Arsenic, surpassing World Health Organization (WHO) permissible limits for drinking water. The method exhibits excellent repeatability and stability, making it ideal for real-world water analysis applications. This innovative sensing platform presents a promising alternative for the sensitive, selective, and robust detection of Arsenic in water. This advancement has the potential to significantly improve water quality monitoring and safeguard public health.Graphical abstract: This work introduces a ground-breaking electrochemical sensor for selectively detecting arsenic, a toxic contaminant, in water. The sensor merges the high sensitivity of stripping voltammetry with the powerful data analysis of chemometrics, achieving exceptional detection limits. By optimizing deposition potential, time, and scan rate, the analytical response is significantly enhanced. A chemometric approach based on principal component analysis (PCA) is implemented to address potential interference from other ions. This technique effectively isolates arsenic signals from background noise and interfering species, leading to superior selectivity and accurate quantification. The proposed method boasts a detection limit of 0.4 nM and a sensitivity of 176.34 µA µM−1 for Arsenic, surpassing World Health Organization (WHO) permissible limits for drinking water. The method exhibits excellent repeatability and stability, making it ideal for real-world water analysis applications. This innovative sensing platform presents a promising alternative for the sensitive, selective, and robust detection of Arsenic in water. This advancement has the potential to significantly improve water quality monitoring and safeguard public health. [ABSTRACT FROM AUTHOR]

The emergence of drug resistance in Streptococcus pneumoniae (Spn) is a global health threat and necessitates discovery of novel therapeutics. The serine acetyltransferase (also known as CysE) is an enzyme of cysteine biosynthesis pathway and is reported to be essential for the survival of several pathogenic bacteria. Therefore, it appears to be a very attractive target for structure–function understanding and inhibitor design. This study describes the molecular cloning of cysE from Spn in the pET21c vector and efforts carried out for expression and purification of active recombinant CysE. Significant expression of recombinant Spn cysE could be achieved in codon optimized BL21(DE3)-RIL strain as opposed to conventional BL21(DE3) strain. Analysis of codon adaptation index (CAI) with levels of eukaryotic genes and prokaryotic cysEs expressed in heterologous E. coli host suggests that codon optimized E. coli BL21(DE3)-RIL may be a better host for expressing genes with low CAI. Here, an efficient protocol has been developed for recovery of recombinant Spn CysE in soluble and biologically active form by the usage of nonionic detergent Triton X-100 at a concentration as low as 1%. Altogether, this study reports a simple strategy for producing functionally active Spn CysE in E. coli. [ABSTRACT FROM AUTHOR]

Multidrug-resistant ESKAPE pathogens are the leading cause of hospital-acquired infections across the globe, posing challenges for clinicians. Random genetic mutations and constant exposure to antibiotics in healthcare settings result in strains resistant to commonly used antibiotics, creating life-threatening conditions. If the magic of "antibiotics" is to be sustained, a new class of antimicrobials against novel targets is urgently needed. This necessitates understanding and identifying novel biochemical pathways and bacterial virulence factors that can be targeted for therapeutic interventions. Keeping in view the unambiguous role of the kinome in bacterial survival and virulence, this review provides a survey of effector bacterial kinases involved in evading host immune responses and drug resistance. The formation of biofilms is a critical feature associated with the pathogenesis and survival of ESKAPE organisms in the hostile host milieu. Hence, kinases involved in the biofilm pathway are also elucidated for clinical relevance. In addition, endeavors in the development of therapeutics against ESKAPE kinases are also summarized to provide direction to researchers pursuing the field., (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)