150 results on '"Antonio Farris"'
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2. Adjuvant anastrozole versus exemestane versus letrozole, upfront or after 2 years of tamoxifen, in endocrine-sensitive breast cancer (FATA-GIM3): a randomised, phase 3 trial
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Sabino De Placido, Ciro Gallo, Michelino De Laurentiis, Giancarlo Bisagni, Grazia Arpino, Maria Giuseppa Sarobba, Ferdinando Riccardi, Antonio Russo, Lucia Del Mastro, Alessio Aligi Cogoni, Francesco Cognetti, Stefania Gori, Jennifer Foglietta, Antonio Frassoldati, Domenico Amoroso, Lucio Laudadio, Luca Moscetti, Filippo Montemurro, Claudio Verusio, Antonio Bernardo, Vito Lorusso, Adriano Gravina, Gabriella Moretti, Rossella Lauria, Antonella Lai, Carmela Mocerino, Sergio Rizzo, Francesco Nuzzo, Paolo Carlini, Francesco Perrone, Antonello Accurso, Biagio Agostara, Michele Aieta, Oscar Alabiso, Maria Grazia Alicicco, Dino Amadori, Laura Amaducci, Gianna Amiconi, Giustino Antuzzi, Mara Ardine, Antonio Ardizzoia, Caterina Aversa, Giuseppe Badalamenti, Sandro Barni, Carlo Basurto, Rossana Berardi, Cinzia Bergamasco, Paolo Bidoli, Claudia Bighin, Edoardo Biondi, Corrado Boni, Karen Borgonovo, Mario Botta, Stefano Bravi, Paolo Bruzzi, Giuseppe Buono, Alfredo Butera, Alessia Caldara, Giampiero Candeloro, Claudia Cappelletti, Cinzia Cardalesi, Elisabetta Carfora, Anna Cariello, Francesco Carrozza, Giacomo Cartenì, Michele Caruso, Virginia Casadei, Claudia Casanova, Luigi Castori, Luigi Cavanna, Giovanna Cavazzini, Marina Cazzaniga, Mario Chilelli, Paolo Chiodini, Silvia Chiorrini, Fortunato Ciardiello, Mariangela Ciccarese, Saverio Cinieri, Mario Clerico, Mariarosa Coccaro, Mario Comande, Claudia Corbo, Giuseppina Cortino, Stefania Cusenza, Gennaro Daniele, Alfonso Maria D'arco, Giuliana D'auria, Claudio Dazzi, Carmine De Angelis, Filippo de Braud, Gianfranco De Feo, Andrea De Matteis, Michele De Tursi, Anna Di Blasio, Giuseppe di Lucca, Liberato Di Lullo, Francesca Di Rella, Gianfranco Di Renzo, Pia Di Stefano, Aida Di Stefano, Anna Diana, Sara Donati, Agnese Fabbri, Alessandra Fabi, Marina Faedi, Gabriella Farina, Antonio Farris, Antonio Febbraro, Palma Fedele, Piera Federico, Francesco Ferraù, Gianluigi Ferretti, Antonella Ferro, Irene Floriani, Rosachiara Forcignanò, Samantha Forciniti, Valeria Forestieri, Gianni Fornari, Michela Frisinghelli, Vittorio Fusco, Giulia Gallizzi, Antonio Galvano, Antonio Gambardella, Angelo Gambi, Vittorio Gebbia, Erika Gervasi, Mara Ghilardi, Alice Giacobino, Giovanni Giardina, Francesco Giotta, Sara Giraudi, Mario Giuliano, Antonino Grassadonia, Donatella Grasso, Federica Grosso, Lorenzo Guizzaro, Pasquale Incoronato, Lorena Incorvaia, Giovanni Iodice, Nicla La Verde, Vincenzo Labonia, Gabriella Landi, Agnese Latorre, Vita Leonardi, Alessia Levaggi, Gennaro Limite, Linda Lina Bascialla, Lorenzo Livi, Evaristo Maiello, Daniela Mandelli, Ilaria Marcon, Daniela Menon, Michele Montedoro, Lucia Moraca, Anna Moretti, Maria Grazia Morritti, Patrizia Morselli, Antonella Mura, Silvia Mura, Michela Musacchio, Alberto Muzio, Donato Natale, Clara Natoli, Cinzia Nigro, Cecilia Nisticò, Antonio Nuzzo, Michele Orditura, Laura Orlando, Carmen Pacilio, Giuliano Palumbo, Raffaella Palumbo, Felice Pasini, Emanuela Paterno, Antonio Pazzola, Silvia Pelliccioni, Matilde Pensabene, Davide Perroni, Angela Pesenti Gritti, Fausto Petrelli, Maria Carmela Piccirillo, Graziella Pinotti, Claudia Pogliani, Davide Poli, Sonia Prader, Francesco Recchia, Daniele Rizzi, Carmen Romano, Rosalba Rossello, Chiara Rossini, Giuseppina Salvucci, Valeria Sanna, Alessandra Santini, Silvana Saracchini, Clementina Savastano, Giovanni Scambia, Francesco Schettini, Paola Schiavone, Alessio Schirone, Elena Seles, Simona Signoriello, Giuseppe Signoriello, Rosa Rita Silva, Antonia Silvestri, Vittorio Simeon, Ilaria Spagnoletti, Stefano Tamberi, Cristina Teragni, Verena Thalmann, Renato Thomas, Guglielmo Thomas, Amelia Tienghi, Nicola Tinari, Vincenza Tinessa, Federica Tomei, Giuseppe Tonini, Valter Torri, Divina Traficante, Marianna Tudini, Monica Turazza, Roberto Vignoli, Maria Giuseppa Vitale, Alessandra Zacchia, Pasquale Zagarese, Alda Zanni, Laura Zavallone, Maria Zavettieri, Alessandra Zoboli, De Placido, S., Gallo, C., De Laurentiis, M., Bisagni, G., Arpino, G., Sarobba, M. G., Riccardi, F., Russo, A., Del Mastro, L., Cogoni, A. A., Cognetti, F., Gori, S., Foglietta, J., Frassoldati, A., Amoroso, D., Laudadio, L., Moscetti, L., Montemurro, F., Verusio, C., Bernardo, A., Lorusso, V., Gravina, A., Moretti, G., Lauria, R., Lai, A., Mocerino, C., Rizzo, S., Nuzzo, F., Carlini, P., Perrone, F., Accurso, A., Agostara, B., Aieta, M., Alabiso, O., Alicicco, M. G., Amadori, D., Amaducci, L., Amiconi, G., Antuzzi, G., Ardine, M., Ardizzoia, A., Aversa, C., Badalamenti, G., Barni, S., Basurto, C., Berardi, R., Bergamasco, C., Bidoli, P., Bighin, C., Biondi, E., Boni, C., Borgonovo, K., Botta, M., Bravi, S., Bruzzi, P., Buono, G., Butera, A., Caldara, A., Candeloro, G., Cappelletti, C., Cardalesi, C., Carfora, E., Cariello, A., Carrozza, F., Carteni, G., Caruso, M., Casadei, V., Casanova, C., Castori, L., Cavanna, L., Cavazzini, G., Cazzaniga, M., Chilelli, M., Chiodini, P., Chiorrini, S., Ciardiello, F., Ciccarese, M., Cinieri, S., Clerico, M., Coccaro, M., Comande, M., Corbo, C., Cortino, G., Cusenza, S., Daniele, G., D'Arco, A. M., D'Auria, G., Dazzi, C., De Angelis, C., de Braud, F., De Feo, G., De Matteis, Ma., De Tursi, M., Di Blasio, A., di Lucca, G., Di Lullo, L., Di Rella, F., Di Renzo, G., Di Stefano, P., Di Stefano, A., Diana, A., Donati, S., Fabbri, A., Fabi, A., Faedi, M., Farina, G., Farris, A., Febbraro, A., Fedele, P., Federico, P., Ferrau, F., Ferretti, G., Ferro, A., Floriani, I., Forcignano, R., Forciniti, S., Forestieri, V., Fornari, G., Frisinghelli, M., Fusco, V., Gallizzi, G., Galvano, A., Gambardella, A., Gambi, A., Gebbia, V., Gervasi, E., Ghilardi, M., Giacobino, A., Giardina, G., Giotta, F., Giraudi, S., Giuliano, M., Grassadonia, A., Grasso, D., Grosso, F., Guizzaro, L., Incoronato, P., Incorvaia, L., Iodice, G., La Verde, N., Labonia, V., Landi, G., Latorre, A., Leonardi, V., Levaggi, A., Limite, G., Lina Bascialla, L., Livi, L., Maiello, E., Mandelli, D., Marcon, I., Menon, D., Montedoro, M., Moraca, L., Moretti, A., Morritti, M. G., Morselli, P., Mura, A., Mura, S., Musacchio, M., Muzio, A., Natale, D., Natoli, C., Nigro, C., Nistico, C., Nuzzo, A., Orditura, M., Orlando, L., Pacilio, C., Palumbo, G., Palumbo, R., Pasini, F., Paterno, E., Pazzola, A., Pelliccioni, S., Pensabene, M., Perroni, D., Pesenti Gritti, A., Petrelli, F., Piccirillo, M. C., Pinotti, G., Pogliani, C., Poli, D., Prader, S., Recchia, F., Rizzi, D., Romano, C., Rossello, R., Rossini, C., Salvucci, G., Sanna, V., Santini, A., Saracchini, S., Savastano, C., Scambia, G., Schettini, F., Schiavone, P., Schirone, A., Seles, E., Signoriello, S., Signoriello, G., Silva, R. R., Silvestri, A., Simeon, V., Spagnoletti, I., Tamberi, S., Teragni, C., Thalmann, V., Thomas, R., Thomas, G., Tienghi, A., Tinari, N., Tinessa, V., Tomei, F., Tonini, G., Torri, V., Traficante, D., Tudini, M., Turazza, M., Vignoli, R., Vitale, M. G., Zacchia, A., Zagarese, P., Zanni, A., Zavallone, L., Zavettieri, M., Zoboli, A., De Placido, Sabino, Gallo, Ciro, De Laurentiis, Michelino, Bisagni, Giancarlo, Arpino, Grazia, Sarobba, Maria Giuseppa, Riccardi, Ferdinando, Russo, Antonio, Del Mastro, Lucia, Cogoni, Alessio Aligi, Cognetti, Francesco, Gori, Stefania, Foglietta, Jennifer, Frassoldati, Antonio, Amoroso, Domenico, Laudadio, Lucio, Moscetti, Luca, Montemurro, Filippo, Verusio, Claudio, Bernardo, Antonio, Lorusso, Vito, Gravina, Adriano, Moretti, Gabriella, Lauria, Rossella, Lai, Antonella, Mocerino, Carmen, Rizzo, Sergio, Nuzzo, Francesco, Carlini, Paolo, Perrone, Francesco, Accurso, Antonello, Agostara, Biagio, Aieta, Michele, Alabiso, Oscar, Alicicco, Maria Grazia, Amadori, Dino, Amaducci, Laura, Amiconi, Gianna, Antuzzi, Giustino, Ardine, Mara, Ardizzoia, Antonio, Aversa, Caterina, Badalamenti, Giuseppe, Barni, Sandro, Basurto, Carlo, Berardi, Rossana, Bergamasco, Cinzia, Bidoli, Paolo, Bighin, Claudia, Biondi, Edoardo, Boni, Corrado, Borgonovo, Karen, Botta, Mario, Bravi, Stefano, Bruzzi, Paolo, Buono, Giuseppe, Butera, Alfredo, Caldara, Alessia, Candeloro, Giampiero, Cappelletti, Claudia, Cardalesi, Cinzia, Carfora, Elisabetta, Cariello, Anna, Carrozza, Francesco, Cartenì, Giacomo, Caruso, Michele, Casadei, Virginia, Casanova, Claudia, Castori, Luigi, Cavanna, Luigi, Cavazzini, Giovanna, Cazzaniga, Marina, Chilelli, Mario, Chiodini, Paolo, Chiorrini, Silvia, Ciardiello, Fortunato, Ciccarese, Mariangela, Cinieri, Saverio, Clerico, Mario, Coccaro, Mariarosa, Comande, Mario, Corbo, Claudia, Cortino, Giuseppina, Cusenza, Stefania, Daniele, Gennaro, D'arco, Alfonso Maria, D'auria, Giuliana, Dazzi, Claudio, De Angelis, Carmine, de Braud, Filippo, De Feo, Gianfranco, De Matteis, Andrea, De Tursi, Michele, Di Blasio, Anna, di Lucca, Giuseppe, Di Lullo, Liberato, Di Rella, Francesca, Di Renzo, Gianfranco, Di Stefano, Pia, Di Stefano, Aida, Diana, Anna, Donati, Sara, Fabbri, Agnese, Fabi, Alessandra, Faedi, Marina, Farina, Gabriella, Farris, Antonio, Febbraro, Antonio, Fedele, Palma, Federico, Piera, Ferraù, Francesco, Ferretti, Gianluigi, Ferro, Antonella, Floriani, Irene, Forcignanò, Rosachiara, Forciniti, Samantha, Forestieri, Valeria, Fornari, Gianni, Frisinghelli, Michela, Fusco, Vittorio, Gallizzi, Giulia, Galvano, Antonio, Gambardella, Antonio, Gambi, Angelo, Gebbia, Vittorio, Gervasi, Erika, Ghilardi, Mara, Giacobino, Alice, Giardina, Giovanni, Giotta, Francesco, Giraudi, Sara, Giuliano, Mario, Grassadonia, Antonino, Grasso, Donatella, Grosso, Federica, Guizzaro, Lorenzo, Incoronato, Pasquale, Incorvaia, Lorena, Iodice, Giovanni, La Verde, Nicla, Labonia, Vincenzo, Landi, Gabriella, Latorre, Agnese, Leonardi, Vita, Levaggi, Alessia, Limite, Gennaro, Lina Bascialla, Linda, Livi, Lorenzo, Maiello, Evaristo, Mandelli, Daniela, Marcon, Ilaria, Menon, Daniela, Montedoro, Michele, Moraca, Lucia, Moretti, Anna, Morritti, Maria Grazia, Morselli, Patrizia, Mura, Antonella, Mura, Silvia, Musacchio, Michela, Muzio, Alberto, Natale, Donato, Natoli, Clara, Nigro, Cinzia, Nisticò, Cecilia, Nuzzo, Antonio, Orditura, Michele, Orlando, Laura, Pacilio, Carmen, Palumbo, Giuliano, Palumbo, Raffaella, Pasini, Felice, Paterno, Emanuela, Pazzola, Antonio, Pelliccioni, Silvia, Pensabene, Matilde, Perroni, Davide, Pesenti Gritti, Angela, Petrelli, Fausto, Piccirillo, Maria Carmela, Pinotti, Graziella, Pogliani, Claudia, Poli, Davide, Prader, Sonia, Recchia, Francesco, Rizzi, Daniele, Romano, Carmen, Rossello, Rosalba, Rossini, Chiara, Salvucci, Giuseppina, Sanna, Valeria, Santini, Alessandra, Saracchini, Silvana, Savastano, Clementina, Scambia, Giovanni, Schettini, Francesco, Schiavone, Paola, Schirone, Alessio, Seles, Elena, Signoriello, Simona, Signoriello, Giuseppe, Silva, Rosa Rita, Silvestri, Antonia, Simeon, Vittorio, Spagnoletti, Ilaria, Tamberi, Stefano, Teragni, Cristina, Thalmann, Verena, Thomas, Renato, Thomas, Guglielmo, Tienghi, Amelia, Tinari, Nicola, Tinessa, Vincenza, Tomei, Federica, Tonini, Giuseppe, Torri, Valter, Traficante, Divina, Tudini, Marianna, Turazza, Monica, Vignoli, Roberto, Vitale, Maria Giuseppa, Zacchia, Alessandra, Zagarese, Pasquale, Zanni, Alda, Zavallone, Laura, Zavettieri, Maria, Zoboli, Alessandra, Mocerino, Carmela, D'Arco, Alfonso Maria, D'Auria, Giuliana, De Placido, S, Gallo, C, De Laurentiis, M, Bisagni, G, Arpino, G, Sarobba, M, Riccardi, F, Russo, A, Del Mastro, L, Cogoni, A, Cognetti, F, Gori, S, Foglietta, J, Frassoldati, A, Amoroso, D, Laudadio, L, Moscetti, L, Montemurro, F, Verusio, C, Bernardo, A, Lorusso, V, Gravina, A, Moretti, G, Lauria, R, Lai, A, Mocerino, C, Rizzo, S, Nuzzo, F, Carlini, P, Perrone, F, Accurso, A, Agostara, B, Aieta, M, Alabiso, O, Alicicco, M, Amadori, D, Amaducci, L, Amiconi, G, Antuzzi, G, Ardine, M, Ardizzoia, A, Aversa, C, Badalamenti, G, Barni, S, Basurto, C, Berardi, R, Bergamasco, C, Bidoli, P, Bighin, C, Biondi, E, Boni, C, Borgonovo, K, Botta, M, Bravi, S, Bruzzi, P, Buono, G, Butera, A, Caldara, A, Candeloro, G, Cappelletti, C, Cardalesi, C, Carfora, E, Cariello, A, Carrozza, F, Carteni, G, Caruso, M, Casadei, V, Casanova, C, Castori, L, Cavanna, L, Cavazzini, G, Cazzaniga, M, Chilelli, M, Chiodini, P, Chiorrini, S, Ciardiello, F, Ciccarese, M, Cinieri, S, Clerico, M, Coccaro, M, Comande, M, Corbo, C, Cortino, G, Cusenza, S, Daniele, G, D'Arco, A, D'Auria, G, Dazzi, C, De Angelis, C, de Braud, F, De Feo, G, De Matteis, A, De Tursi, M, Di Blasio, A, di Lucca, G, Di Lullo, L, Di Rella, F, Di Renzo, G, Di Stefano, P, Di Stefano, A, Diana, A, Donati, S, Fabbri, A, Fabi, A, Faedi, M, Farina, G, Farris, A, Febbraro, A, Fedele, P, Federico, P, Ferrau, F, Ferretti, G, Ferro, A, Floriani, I, Forcignano, R, Forciniti, S, Forestieri, V, Fornari, G, Frisinghelli, M, Fusco, V, Gallizzi, G, Galvano, A, Gambardella, A, Gambi, A, Gebbia, V, Gervasi, E, Ghilardi, M, Giacobino, A, Giardina, G, Giotta, F, Giraudi, S, Giuliano, M, Grassadonia, A, Grasso, D, Grosso, F, Guizzaro, L, Incoronato, P, Incorvaia, L, Iodice, G, La Verde, N, Labonia, V, Landi, G, Latorre, A, Leonardi, V, Levaggi, A, Limite, G, Lina Bascialla, L, Livi, L, Maiello, E, Mandelli, D, Marcon, I, Menon, D, Montedoro, M, Moraca, L, Moretti, A, Morritti, M, Morselli, P, Mura, A, Mura, S, Musacchio, M, Muzio, A, Natale, D, Natoli, C, Nigro, C, Nistico, C, Nuzzo, A, Orditura, M, Orlando, L, Pacilio, C, Palumbo, G, Palumbo, R, Pasini, F, Paterno, E, Pazzola, A, Pelliccioni, S, Pensabene, M, Perroni, D, Pesenti Gritti, A, Petrelli, F, Piccirillo, M, Pinotti, G, Pogliani, C, Poli, D, Prader, S, Recchia, F, Rizzi, D, Romano, C, Rossello, R, Rossini, C, Salvucci, G, Sanna, V, Santini, A, Saracchini, S, Savastano, C, Scambia, G, Schettini, F, Schiavone, P, Schirone, A, Seles, E, Signoriello, S, Signoriello, G, Silva, R, Silvestri, A, Simeon, V, Spagnoletti, I, Tamberi, S, Teragni, C, Thalmann, V, Thomas, R, Thomas, G, Tienghi, A, Tinari, N, Tinessa, V, Tomei, F, Tonini, G, Torri, V, Traficante, D, Tudini, M, Turazza, M, Vignoli, R, Vitale, M, Zacchia, A, Zagarese, P, Zanni, A, Zavallone, L, Zavettieri, M, and Zoboli, A
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Oncology ,Receptor, ErbB-2 ,Settore MED/06 - Oncologia Medica ,letrozole ,law.invention ,Adjuvant anastrozole ,chemistry.chemical_compound ,0302 clinical medicine ,Randomized controlled trial ,Exemestane ,law ,exemestane ,tamoxifen ,breast cancer ,Antineoplastic Combined Chemotherapy Protocols ,030212 general & internal medicine ,Aromatase Inhibitors ,Letrozole ,Hazard ratio ,Middle Aged ,Receptors, Estrogen ,Tolerability ,Chemotherapy, Adjuvant ,030220 oncology & carcinogenesis ,Female ,Receptors, Progesterone ,Breast Neoplasm ,Human ,medicine.drug ,medicine.medical_specialty ,Socio-culturale ,Anastrozole ,Breast Neoplasms ,Disease-Free Survival ,Drug Administration Schedule ,03 medical and health sciences ,Breast cancer ,Internal medicine ,medicine ,Aromatase Inhibitor ,Humans ,Aged ,Antineoplastic Combined Chemotherapy Protocol ,Androstadiene ,business.industry ,medicine.disease ,Androstadienes ,chemistry ,business ,Tamoxifen - Abstract
Background: Uncertainty exists about the optimal schedule of adjuvant treatment of breast cancer with aromatase inhibitors and, to our knowledge, no trial has directly compared the three aromatase inhibitors anastrozole, exemestane, and letrozole. We investigated the schedule and type of aromatase inhibitors to be used as adjuvant treatment for hormone receptor-positive early breast cancer. Methods: FATA-GIM3 is a multicentre, open-label, randomised, phase 3 trial of six different treatments in postmenopausal women with hormone receptor-positive early breast cancer. Eligible patients had histologically confirmed invasive hormone receptor-positive breast cancer that had been completely removed by surgery, any pathological tumour size, and axillary nodal status. Key exclusion criteria were hormone replacement therapy, recurrent or metastatic disease, previous treatment with tamoxifen, and another malignancy in the previous 10 years. Patients were randomly assigned in an equal ratio to one of six treatment groups: oral anastrozole (1 mg per day), exemestane (25 mg per day), or letrozole (2·5 mg per day) tablets upfront for 5 years (upfront strategy) or oral tamoxifen (20 mg per day) for 2 years followed by oral administration of one of the three aromatase inhibitors for 3 years (switch strategy). Randomisation was done by a computerised minimisation procedure stratified for oestrogen receptor, progesterone receptor, and HER2 status; previous chemotherapy; and pathological nodal status. Neither the patients nor the physicians were masked to treatment allocation. The primary endpoint was disease-free survival. The minimum cutoff to declare superiority of the upfront strategy over the switch strategy was assumed to be a 2% difference in disease-free survival at 5 years. Primary efficacy analyses were done by intention to treat; safety analyses included all patients for whom at least one safety case report form had been completed. Follow-up is ongoing. This trial is registered with the European Clinical Trials Database, number 2006-004018-42, and ClinicalTrials.gov, number NCT00541086. Findings: Between March 9, 2007, and July 31, 2012, 3697 patients were enrolled into the study. After a median follow-up of 60 months (IQR 46â72), 401 disease-free survival events were reported, including 211 (11%) of 1850 patients allocated to the switch strategy and 190 (10%) of 1847 patients allocated to upfront treatment. 5-year disease-free survival was 88·5% (95% CI 86·7â90·0) with the switch strategy and 89·8% (88·2â91·2) with upfront treatment (hazard ratio 0·89, 95% CI 0·73â1·08; p=0·23). 5-year disease-free survival was 90·0% (95% CI 87·9â91·7) with anastrozole (124 events), 88·0% (85·8â89·9) with exemestane (148 events), and 89·4% (87·3 to 91·1) with letrozole (129 events; p=0·24). No unexpected serious adverse reactions or treatment-related deaths occurred. Musculoskeletal side-effects were the most frequent grade 3â4 events, reported in 130 (7%) of 1761 patients who received the switch strategy and 128 (7%) of 1766 patients who received upfront treatment. Grade 1 musculoskeletal events were more frequent with the upfront schedule than with the switch schedule (924 [52%] of 1766 patients vs 745 [42%] of 1761 patients). All other grade 3â4 adverse events occurred in less than 2% of patients in either group. Interpretation: 5 years of treatment with aromatase inhibitors was not superior to 2 years of tamoxifen followed by 3 years of aromatase inhibitors. None of the three aromatase inhibitors was superior to the others in terms of efficacy. Therefore, patient preference, tolerability, and financial constraints should be considered when deciding the optimal treatment approach in this setting. Funding: Italian Drug Agency.
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- 2018
3. Update of the Phase III trial ‘GRETA’ of surgery and tamoxifen versus tamoxifen alone for early breast cancer in elderly women
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Antonio Farris, Giulio Isola, Alfonso Pluchinotta, Imma Capasso, Giorgio Mustacchi, and A. Scanni
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Oncology ,Cancer Research ,medicine.medical_specialty ,Antineoplastic Agents, Hormonal ,Breast Neoplasms ,Breast cancer ,Internal medicine ,medicine ,Overall survival ,Delayed surgery ,Humans ,Endocrine system ,Neoplasm Metastasis ,Aged ,Neoplasm Staging ,Randomized Controlled Trials as Topic ,Early breast cancer ,Aged, 80 and over ,business.industry ,General Medicine ,medicine.disease ,Surgery ,Tamoxifen ,Treatment Outcome ,Clinical Trials, Phase III as Topic ,Chemotherapy, Adjuvant ,Female ,business ,medicine.drug - Abstract
Background: In the Phase III ‘GRETA’ trial 474 women aged ≥70 years with early breast cancer were randomly assigned to surgery plus tamoxifen for 5 years or tamoxifen alone for 5 years. This is a long-term update. Patients & methods: Focusing on patients still alive in 2003, outcome end points has been recalculated. Results: Median distant metastases disease-free survival is longer with tamoxifen alone for 5 years; (48.8 vs 37.9 months; p = 0.009). No difference was found in distant metastases rate, disease-free survival, breast cancer and overall survival. Conclusion: Primary endocrine treatment until the the best response, followed by minimal surgery and prosecution endocrine treatment for 5–10 years is a suitable option for elderly breast cancer patients. Delayed surgery does not prejudice overall survival.
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- 2015
4. Wine quality improvement through the combined utilisation of yeast hulls andCandida zemplinina/Saccharomyces cerevisiaemixed starter cultures
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Severino Zara, Marilena Budroni, Giovanni Antonio Farris, Ilaria Mannazzu, M.B. Pinna, M. Murru, Giacomo Zara, and A. Del Caro
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Wine ,education.field_of_study ,Population ,food and beverages ,Horticulture ,Biology ,Yeast ,Winery ,Candida zemplinina ,Yeast in winemaking ,chemistry.chemical_compound ,chemistry ,Glycerol ,Food science ,education ,Winemaking - Abstract
Background and Aims Interactions between different yeast species used as starters may lead to inconsistent results in mixed fermentations. The aim of this study was to assess the influence of different nutrients on the association between a wine strain of Candida zemplinina (CDZ1) and a commercial wine strain of Saccharomyces cerevisiae (EC1118) in mixed culture fermentations. Methods and Results Laboratory-scale fermentations were carried out by inoculating CDZ1 and, after 3 days, EC1118 with the simultaneous addition of diammonium phosphate, yeast hulls, ergosterol and oleic acid, each provided separately. The addition of yeast hulls resulted in a higher cell population of CDZ1 and in a higher glycerol concentration of wine as compared with that of the other fermentations. Pilot-scale fermentations, carried out in a commercial winery, confirmed that the winemaking protocol based on the use of the mixed starters CDZ1/EC1118 with added yeast hulls results in wine with a higher glycerol content, more structured and persistent. Conclusions Addition of yeast hulls improved the fermentative performance of the mixed starters CDZ1/EC1118. Significance of the Study The high glycerol content of wines made with the combined use of yeast hulls and CDZ1/EC1118 mixed starters increases wine softness and body.
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- 2014
5. Short-term effects on sheep pastureland due to grazing abandonment in a Western Mediterranean island ecosystem: A multidisciplinary approach
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Pietrino Deiana, Emmanuele Farris, Rossella Speranza Filigheddu, Giovanni Antonio Farris, and Giovanni Garau
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geography ,geography.geographical_feature_category ,Ecology ,Soil organic matter ,Species diversity ,Plant community ,Biology ,Pasture ,Agronomy ,Grazing ,Species richness ,Soil fertility ,Conservation grazing ,Nature and Landscape Conservation - Abstract
Changes of pasture communities consequent to management practices resulting from land abandonment considerably affect the structure and function of the ecosystem. This study analyses the consequences of grazing abandonment in terms of plant and soil microbial diversity and fertility, on a Mediterranean upland sheep pasture, over a short period (five years). Grazing was experimentally excluded by fencing ten 10×10 m permanent plots within an area that had supported grazing until 2000, by 0.23 sheep ha−1. Plant and soil microbial communities and physicochemical parameters were monitored within the fenced and unfenced control plots, during three sampling times from 2000 (before the fencing) to 2005. Grazing cessation notably altered the floral composition, with an average dissimilarity of 96.7% between the vegetation communities, over five years. No significant change occurred in the control plots that were grazed throughout the sampling period. This work highlighted that, over a short term, the structural change in the specific plant composition affected only the grass species, confirming that grazing favours the small-sized species over the annual species. Further, it was evident that species groups of conservational and phytogeographic interest, like the endemic and Mediterranean-Atlantic species, tended to disappear with pasture abandonment and were substituted by more widespread species throughout the Mediterranean or even the world. Pasture abandonment was accompanied by an increase of soil pH and a decrease in soil organic matter and soil nitrogen. The microbial parameters recorded at three different sampling times revealed a substantial effect of the plant community, or the time of grazing abandonment, on soil microbial abundance and diversity. Considerable importance is given to the consequences of pasture abandonment on the conservation of plant and microbial diversity and on soil fertility.
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- 2010
6. Randomized Trial of Intravenous Iron Supplementation in Patients With Chemotherapy-Related Anemia Without Iron Deficiency Treated With Darbepoetin Alfa
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Francesco Di Costanzo, Salvatore Del Prete, Salvatore Siena, Paolo Pedrazzoli, Paola Pozzi, Antonio Farris, Alessandro Pappalardo, Roberto Labianca, Giuseppe Colucci, Giuseppe Fornarini, Clara Bianchessi, Alessandra Fabi, E. Crucitta, Federica Apolloni, Alberto Desogus, Antonio Santo, Simona Secondino, Daris Ferrari, Teresa Gamucci, and Filomena Del Gaizo
- Subjects
Male ,Cancer Research ,medicine.medical_specialty ,Randomization ,Darbepoetin alfa ,Anemia ,Iron ,medicine.medical_treatment ,Antineoplastic Agents ,Gastroenterology ,law.invention ,Randomized controlled trial ,law ,Neoplasms ,Internal medicine ,medicine ,Humans ,Infusions, Intravenous ,Erythropoietin ,Chemotherapy ,business.industry ,Iron deficiency ,medicine.disease ,Surgery ,Clinical trial ,Oncology ,Iron-deficiency anemia ,Hematinics ,Female ,business ,medicine.drug - Abstract
Purpose Unresponsiveness to erythropoiesis-stimulating agents, occurring in 30% to 50% of patients, is a major limitation to the treatment of chemotherapy-related anemia. We have prospectively evaluated whether intravenous iron can increase the proportion of patients with chemotherapy-related anemia who respond to darbepoetin. Patients and Methods Between December 2004 and February 2006, 149 patients with lung, gynecologic, breast, and colorectal cancers and ≥ 12 weeks of planned chemotherapy were enrolled from 33 institutions. Patients were required to have hemoglobin ≤ 11 g/L and no absolute or functional iron deficiency. All patients received darbepoetin 150 μg subcutaneously once weekly for 12 weeks and were randomly assigned to sodium ferric gluconate 125 mg intravenously (IV) weekly for the first 6 weeks (n = 73) or no iron (n = 76). Primary end point of the study was the percentage of patients achieving hematopoietic response (hemoglobin ≥ 12 g/dL or ≥ 2 g/dL increase). Results Hematopoietic response by intention-to-treat analysis was 76.7% (95%CI, 65.4% to 85.8%) in the darbepoetin/iron group and 61.8% (95%CI, 50.0% to 72.7%) in the darbepoetin group (P = .0495). Among patients fulfilling eligibility criteria and having received at least four darbepoetin administrations, hematopoietic responses in the darbepoetin/iron group (n = 53) and in the darbepoetin-only group (n = 50) were 92.5% (95% CI, 81.8% to 97.9%) and 70% (95% CI, 55.4% to 82.1%), respectively (P = .0033). Increase of hemoglobin during treatment period showed a time profile favoring darbepoetin/iron with statistically significant effect from week 5 on. The safety profile was comparable in the two arms. Conclusion In patients with chemotherapy-related anemia and no iron deficiency, IV iron supplementation significantly reduces treatment failures to darbepoetin without additional toxicity.
- Published
- 2008
7. Correlation between cell lipid content, gene expression and fermentative behaviour of two Saccharomyces cerevisiae wine strains
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Simona Belviso, Giovanni Antonio Farris, Giacomo Zara, Luca Bardi, Severino Zara, and Marilena Budroni
- Subjects
Saccharomyces cerevisiae ,Gene Expression ,Wine ,Biology ,Applied Microbiology and Biotechnology ,Industrial Microbiology ,chemistry.chemical_compound ,Gene Expression Regulation, Fungal ,Gene expression ,DNA, Fungal ,chemistry.chemical_classification ,Fatty Acids ,food and beverages ,Fatty acid ,Fructose ,Lipid metabolism ,General Medicine ,biology.organism_classification ,Lipids ,Yeast ,chemistry ,Biochemistry ,Fermentation ,Biotechnology - Abstract
Aim: To verify a possible correlation between cell lipid composition, expression of key genes in lipid metabolism and fermentative behaviour of Saccharomyces cerevisiae wine strains. Methods and Results: The fermentative abilities of two commercial wine strains of S. cerevisiae were tested under stressful conditions. Cell number, glucose and fructose concentrations, expression of ACS1, ACS2, ACC1, OLE1, ERG9, ERG10, ARE1 and ARE2 and lipid content were evaluated. The strain that failed to complete the fermentation had lower amounts of C16:1 and C16:0 fatty acids at the beginning of fermentation (0 h) and late logarithmic phase (72 h). While the amount of C18:1 in this strain was lower than that in the strain that completed the fermentation at 0 h, same levels were observed for both strains at 72 h. The sterol levels were generally higher in the strain that failed to complete the fermentation. Gene expression generally increased from the beginning of the fermentation to the late logarithmic phase in both strains. Conclusion: A positive correlation between good fermentative ability, elevated fatty acid content and ACC1 gene expression has been identified. Significance and Impact of the Study: The cell lipid content at the time of inoculum and expression of ACC1 gene of starter strains should be carefully considered in order to identify the possible stuck/sluggish fermentations.
- Published
- 2008
8. Probiotic Preparation Has the Capacity To Hydrolyze Proteins Responsible for Wheat Allergy
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Enrico Scala, Carlo Giuseppe Rizzello, Francesco Michelangelo Turrini, Giovanni Antonio Farris, Claudio De Simone, Maria De Angelis, and Marco Gobbetti
- Subjects
probiotics ,gluten ,fermentation ,Globulin ,Galectin 3 ,Flour ,Wheat flour ,Wheat Hypersensitivity ,Microbiology ,poteomics ,mass spectrometry ,allergens ,law.invention ,Probiotic ,Pepsin ,law ,Albumins ,medicine ,Humans ,Electrophoresis, Gel, Two-Dimensional ,Food science ,Triticum ,Gel electrophoresis ,chemistry.chemical_classification ,biology ,Chemistry ,Hydrolysis ,Probiotics ,food and beverages ,Immunoglobulin E ,medicine.disease ,Pepsin A ,Yeast ,Enzyme ,Biochemistry ,Immunoglobulin G ,Fermentation ,Pancreatin ,Food Microbiology ,biology.protein ,Electrophoresis, Polyacrylamide Gel ,Wheat allergy ,Peptide Hydrolases ,Food Science - Abstract
This study was aimed at showing the capacity of probiotic VSL#3 to hydrolyze wheat flour allergens. Hydrolysis was investigated either by the use of baker's yeast bread treated with digestive enzymes and VSL#3, an experimental design that mimicked the activity of probiotics during gut colonization, or by the use of VSL#3 as a starter for dough fermentation, an experimental design that mimicked the predigestion of wheat flour proteins during food processing. Albumins, globulins, and gliadins extracted from wheat flour and chemically acidified and started dough and total proteins extracted from breads were analyzed by immunoblotting with pooled sera from patients with an allergy to wheat. Hydrolysis of wheat flour proteins was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional electrophoresis (2DE). Mass spectrometry matrix-assisted laser desorption and ionization-time of flight was used to identify some immunoglobulin E (IgE)-binding proteins. As shown by immunoblotting with sera from allergic patients, several IgE-binding proteins persisted after treatment of baker's yeast bread by pepsin and pancreatin. The signal of all these IgE-binding proteins disappeared after further treatment by VSL#3. As shown by SDS-PAGE and related immunoblotting and 2DE analyses, when VSL#3 was used as a starter for bread making, it caused a marked degradation of wheat proteins, including some IgE-binding proteins such as the putative transcription factor APFI and wheat alpha-amylase inhibitors. Indeed, the IgE-binding profile of the bread manufactured by VSL#3 was largely different from that of baker's yeast bread. The IgE-binding proteins that persisted in the bread made with VSL#3 were completely degraded by pepsin and pancreatin.
- Published
- 2007
9. Lactic acid fermentation as a tool to enhance the antioxidant properties of Myrtus communis berries
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Carlo Giuseppe Rizzello, José Antonio Curiel, Barbara Marzani, Pasquale Filannino, Marco Gobbetti, Daniela Pinto, and Giovanni Antonio Farris
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DPPH ,Linoleic acid ,myrtle ,antioxidant activity ,Antioxidants ,chemistry.chemical_compound ,Mice ,applied microbiology and biotechnology ,Animals ,fermentation ,lactic acid bacteria ,biotechnology ,bioengineering ,Food science ,Lactic Acid ,Myrtus communis ,biology ,Research ,food and beverages ,Fibroblasts ,biology.organism_classification ,Myrtus ,Lactic acid ,AGR/16 Microbiologia agraria ,chemistry ,Biochemistry ,Fruit ,Fermentation ,Myricetin ,Reactive Oxygen Species ,Lactobacillus plantarum ,Lactic acid fermentation - Abstract
Background: Myrtle (Myrtus communis L.) is a medicinal and aromatic plant belonging to Myrtaceae family, which is largely diffused in the Mediterranean areas and mainly cultivated in Tunisia and Italy. To the best of our knowledge, no studies have already considered the use of the lactic acid fermentation to enhance the functional features of M. communis. This study aimed at using a selected lactic acid bacterium for increasing the antioxidant features of myrtle berries, with the perspective of producing a functional ingredient, dietary supplement or pharmaceutical preparation. The antioxidant activity was preliminarily evaluated through in vitro assays, further confirmed through ex vivo analysis on murine fibroblasts, and the profile of phenol compounds was characterized. Results: Myrtle berries homogenate, containing yeast extract (0.4%, wt/vol), was fermented with Lactobacillus plantarum C2, previously selected from plant matrix. Chemically acidified homogenate, without bacterial inoculum and incubated under the same conditions, was used as the control. Compared to the control, fermented myrtle homogenate exhibited a marked antioxidant activity in vitro. The radical scavenging activity towards DPPH increased by 30%, and the inhibition of linoleic acid peroxidation was twice. The increased antioxidant activity was confirmed using Balb 3 T3 mouse fibroblasts, after inducing oxidative stress, and determining cell viability and radical scavenging activity through MTT and DCFH-DA assays, respectively. The lactic acid fermentation allowed increased concentrations of total phenols, flavonoids and anthocyanins, which were 5–10 times higher than those found for the non-fermented and chemically acidified control. As shown by HPLC analysis, the main increases were found for gallic and ellagic acids, and flavonols (myricetin and quercetin). The release of these antioxidant compounds would be strictly related to the esterase activities of L. plantarum. Conclusions: The lactic acid fermentation of myrtle berries is a suitable tool for novel applications as functional food dietary supplements or pharmaceutical preparations.
- Published
- 2015
10. Small Cell Lung Cancer in a Young Patient with Osteopetrosis
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Maria Antonietta Pinna, Giovanni Sanna, Carlo Putzu, Maria Cossu Rocca, Paolo Cossu Rocca, S Costantino, Maria Giuseppa Sarobba, Giovanni Fadda, Davide Adriano Santeufemia, and Antonio Farris
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Adult ,Male ,Cancer Research ,Pathology ,medicine.medical_specialty ,Lung Neoplasms ,Biopsy ,Disease ,030218 nuclear medicine & medical imaging ,03 medical and health sciences ,0302 clinical medicine ,Occlusion ,medicine ,Humans ,Neoplasm ,Carcinoma, Small Cell ,Lung cancer ,business.industry ,Reabsorption ,Osteopetrosis ,General Medicine ,medicine.disease ,Radiography ,medicine.anatomical_structure ,Oncology ,030220 oncology & carcinogenesis ,Toxicity ,Bone marrow ,business - Abstract
Background Osteopetrosis or Albers-Schonberg's disease is a heterogeneous group of rare hereditary troubles of the bone characterized by bone sclerosis due to an alteration of the bone reabsorption mediated by osteoclasts. The defect in the osteoclastic activity is responsible for complete or partial medullary cavities occlusion, with consequent reduced hemopoiesis, and for the excessive fragility of the affected bone segments. Case report We reported the case of a young man of 31 years affected by osteopetrosis in which a small cell lung cancer developed. Results Small cell lung cancer is a particularly rare neoplasm in the young, and even though it is highly sensitive to chemotherapeutic treatment its prognosis remains poor. The greatest clinical problem connected with chemotherapeutic treatment of patients affected by osteopetrosis is the variability of the reduction of their bone marrow reserve, which could expose them to an excessive hematological toxicity caused by the therapy. Conclusions The adoption of suitable prophylactic measures, such as the use of growth factors and drugs selected in relation to their toxicity or given in reduced doses, should be appropriately considered in these subjects.
- Published
- 2006
11. Molecular characterization of lactic acid bacteria from sourdough breads produced in Sardinia (Italy) and multivariate statistical analyses of results
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Pasquale Catzeddu, Eugenio Parente, Manuela Sanna, Enrica Mura, and Giovanni Antonio Farris
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Lactobacillus sanfranciscensis ,Lactobacillus pentosus ,Polymerase Chain Reaction ,Applied Microbiology and Biotechnology ,Microbiology ,chemistry.chemical_compound ,Species Specificity ,RNA, Ribosomal, 16S ,Lactobacillus ,Food science ,Ribosomal DNA ,Ecology, Evolution, Behavior and Systematics ,Genetics ,biology ,food and beverages ,Bread ,16S ribosomal RNA ,biology.organism_classification ,Random Amplified Polymorphic DNA Technique ,RAPD ,Lactic acid ,RNA, Bacterial ,Italy ,chemistry ,Multivariate Analysis ,Bacteria - Abstract
The objective of this work was to investigate the structure and diversity of lactic acid bacteria (LAB) communities in sourdough used for the production of traditional breads (Carasau, Moddizzosu, Spianata, Zichi) in Sardinia. 16S rDNA sequencing and Randomly Amplified Polymorphic DNA (RAPD-PCR) was applied for the identification and typing of the LAB isolated from 25 samples of sourdoughs. Multivariate statistical techniques were applied to RAPD-PCR pattern to study the biological diversity of sourdough samples. Twelve different species of LAB were identified, and most isolates were classified as facultative heterofermentative lactobacilli. Lactobacillus pentosus dominated the lactic microflora of many samples while Lactobacillus sanfranciscensis was isolated only from a limited number of samples. Although heterofermentative species represented between between 30% and 60% of the isolates in Carasau, Spianata and Zichi sourdoughs, only 2% of the isolates from Moddizzosu sourdoughs were identified as heterofermentative LAB. RAPD-PCR with a single primer followed by cluster analysis did not allow the identification of the isolates at the species level. However, a multidimensional scaling/bootstrapping approach on the RAPD-PCR patterns uncovered the diversity of the LAB communities of LAB showing differences both within and between bread types.
- Published
- 2006
12. Modulation of 5-fluorouracil as adjuvant systemic chemotherapy in colorectal cancer: the IGCS-COL multicentre, randomised, phase III study
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Sergio Palmeri, F. De Vita, G Persico, F. Perrone, Corrado Ficorella, Luigi Manzione, Chiara Carlomagno, Massimo Lopez, Vincenzo Adamo, Ciro Gallo, Angelo Raffaele Bianco, Enrico Cortesi, Antonio Farris, C Iannace, G P Ianniello, Silvano Palazzo, G Pistillucci, G Paoletti, S. De Placido, M Gemini, Nicolo' Gebbia, DE PLACIDO, Sabino, Lopez, M, Carlomagno, Chiara, Paoletti, G, Palazzo, S, Manzione, L, Iannace, C, Ianniello, Gp, DE VITA, F, Ficorella, C, Farris, A, Pistillucci, G, Gemini, M, Cortesi, E, Adamo, V, Gebbia, N, Palmeri, S, Gallo, C, Perrone, F, Persico, G, Bianco, ANGELO RAFFAELE, DE PLACIDO, S, Carlomagno, C, DE VITA, Ferdinando, Gallo, Ciro, and Bianco, Ar
- Subjects
Adult ,Male ,Cancer Research ,medicine.medical_specialty ,Randomization ,5-fluorouracil modulation ,adjuvant chemotherapy ,colorectal cancer ,medicine.drug_class ,Colorectal cancer ,Leucovorin ,Antimetabolite ,Gastroenterology ,Disease-Free Survival ,Folinic acid ,RECTAL CANCER ,COLON ,Internal medicine ,Clinical Studies ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,Mucositis ,Humans ,Aged ,business.industry ,Hazard ratio ,Middle Aged ,medicine.disease ,Surgery ,Treatment Outcome ,Levamisole ,Oncology ,Chemotherapy, Adjuvant ,Fluorouracil ,Vomiting ,Female ,medicine.symptom ,Colorectal Neoplasms ,business ,medicine.drug - Abstract
The aims of this multicentre, randomised phase III trial were to evaluate: (1) the role of levamisol (LEV); and (2) the role of folinic acid (FA), added to 5-fluorouracil (5FU) in the adjuvant treatment of colorectal cancer. Patients with histologically proven, radically resected stage II or III colon or rectal cancer were eligible. The study had a 2x2 factorial design with four treatment arms: (a) 5FU alone, (b) 5FU+LEV, (c) 5FU+FA, (d) 5FU+LEV+FA, and two planned comparisons, testing the role of LEV and of FA, respectively. From March 1991, to September 1998, 1327 patients were randomised. None of the two comparisons resulted in a significant disease-free (DFS) or overall (OAS) survival advantage. The hazard ratio (HR) of relapse was 0.89 (95\% confidence intervals (CI): 0.73-1.09) for patients receiving FA and 0.99 (95\% CI 0.80-1.21) for those receiving LEV; corresponding HRs of death were 1.02 (95\% CI: 0.80-1.30) and 0.94 (95\% CI 0.73-1.20). Nonhaematological toxicity (all grade vomiting, diarrhoea, mucositis, congiuntivitis, skin, fever and fatigue) was significantly worse with FA, while all other toxicities were similar. In the present trial, there was no evidence that the addition of FA or LEV significantly prolongs DFS and OAS of radically resected colorectal cancer patients..
- Published
- 2005
13. Spectrum and prevalence ofBRCA1 andBRCA2 germline mutations in Sardinian patients with breast carcinoma through hospital-based screening
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Francesco Tanda, Antonio Cossu, Antonio Farris, P. Baldinu, Giuseppe Palmieri, Antonio Contu, Mario Budroni, Marina Pisano, Grazia Palomba, and Maria F. Dedola
- Subjects
Adult ,Oncology ,Cancer Research ,medicine.medical_specialty ,endocrine system diseases ,Genes, BRCA2 ,Genes, BRCA1 ,Breast Neoplasms ,medicine.disease_cause ,Breast cancer ,Germline mutation ,Internal medicine ,Ovarian carcinoma ,Prevalence ,medicine ,Humans ,Male Breast Carcinoma ,skin and connective tissue diseases ,Germ-Line Mutation ,Aged ,Genetic testing ,Mutation ,medicine.diagnostic_test ,business.industry ,Cancer ,Middle Aged ,medicine.disease ,Immunology ,Female ,Breast carcinoma ,business - Abstract
BACKGROUND Factors that are predictive of carrying BRCA1 and BRCA2 germline mutations in patients with breast carcinoma are awaited widely. The genetically homogeneous Sardinian population may be useful for defining the role of such genetic alterations further through a clinical evaluation program. METHODS One hundred two of 659 patients with breast carcinoma (15.5%) who were collected consecutively had a family history of breast carcinoma and were screened for BRCA1/2 mutations by denaturing high-performance liquid chromatography and DNA sequencing. RESULTS Three deleterious germline BRCA1/2 mutations were detected in 15 of 102 families (14.7%), including 13 families (86.7%) with BRCA2 mutations and 2 families (13.3%) with BRCA1 mutations. A single variant, BRCA2-8765delAG, was the most recurrent mutation in the series and was found in 12 of 102 families (11.8%) and in 18 of 657 patients (2.7%). The average age at diagnosis was significantly younger in families with BRCA1/2 mutations (48.6 yrs) compared with the age of patients who had no detectable mutation (52.9 yrs; P = 0.039). Moreover, BRCA1/2 mutations were found at a significantly higher rate in families who had at least 1 member with ovarian carcinoma or male breast carcinoma (5 of 12 families; 41.7%) than in families without such an association (10 of 90 families; 11.1%; P = 0.003). CONCLUSIONS BRCA2 mutations were approximately 6 times more prevalent than BRCA1 mutations. A diagnosis of breast carcinoma before age 50 years, ovarian carcinoma, male breast carcinoma, and 3 affected generations all were associated significantly with BRCA1/2 mutations. Although the current findings provided further support for the hypothesis that additional breast carcinoma susceptibility genes remain to be identified, such indicators of the presence of BRCA1/2 mutations may be useful in counseling patients about undergoing genetic testing. Cancer 2005. © 2005 American Cancer Society.
- Published
- 2005
14. Biweekly oxaliplatin combined with oral capecitabine (OXXEL regimen) as first-line treatment of metastatic colorectal cancer patients: a Southern Italy Cooperative Oncology Group phase II study
- Author
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Pasquale Comella, Salvatore Tafuto, Bruno Massidda, Luigi Maiorino, L. De Lucia, D. Natale, Antonio Farris, G. De Cataldis, Rossana Casaretti, S. Palmeri, P COMELLA, BMASSIDDA, PALMERI S, AFARRIS, LDE LUCIA, DNATALE, LMAIORINO, STAFUTO, GDE CATALDIS, and RCASARETTI
- Subjects
Adult ,Male ,Antimetabolites, Antineoplastic ,Cancer Research ,medicine.medical_specialty ,Lung Neoplasms ,Organoplatinum Compounds ,Colorectal cancer ,Phases of clinical research ,Antineoplastic Agents ,Toxicology ,Deoxycytidine ,Gastroenterology ,Disease-Free Survival ,Capecitabine ,Internal medicine ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,Carcinoma ,Humans ,Pharmacology (medical) ,Peritoneal Neoplasms ,Aged ,Aged, 80 and over ,Pharmacology ,Performance status ,business.industry ,Liver Neoplasms ,Middle Aged ,medicine.disease ,Oxaliplatin ,Surgery ,Regimen ,Italy ,Oncology ,Fluorouracil ,Lymphatic Metastasis ,Female ,Colorectal Neoplasms ,business ,medicine.drug - Abstract
Oxaliplatin 100 mg/m(2) iv on day 1, and capecitabine 1,000 mg/m(2) orally bid from day 1 (evening) to day 11 (morning) were administered every 2 weeks (OXXEL regimen) to 38 patients as first-line treatment for metastatic colorectal carcinoma. A total of 318 cycles were administered, with a median of 8 (range, 4-12) cycles per patient. Response rate (RR) was 45% (95% confidence interval (CI), 29%-62%), with 7 complete responses and 10 partial responses; furthermore, 12 patients showed a stable disease, so that a disease control was achieved in 29 (76%) patients. RR was greater among patients with performance status 0 (52%), without weight loss (52%), younger than 65 years (50%), and previously unexposed to adjuvant chemotherapy (48%), while no correlation was found with the actually delivered oxaliplatin dose intensity. Overall, haematological side effects were negligible, with no case of grade 4 toxicity, and only one patient suffering from an episode of grade 3 neutropenic fever. Severe anaemia occurred in 4 (11%) patients, and grade 3 neuropathy affected 9 (24%) patients. Median progression-free survival was 7.9 (95% CI, 6.2-9.6) months, and median overall survival has not been reached yet. In conclusion, the OXXEL regimen resulted safe and active, and it deserves further evaluation in metastatic colorectal cancer patients.
- Published
- 2005
15. A tailored regimen including capecitabine and oxaliplatin for treating elderly patients with metastatic colorectal carcinoma
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Rossana Casaretti, A. Gambardella, Salvatore Tafuto, Vito Lorusso, Silvana Leo, D. Natale, Luigi Maiorino, Antonio Farris, Pasquale Comella, and Bruno Massidda
- Subjects
medicine.medical_specialty ,Colorectal cancer ,Nausea ,business.industry ,Hematology ,medicine.disease ,Gastroenterology ,Oxaliplatin ,Surgery ,Clinical trial ,Capecitabine ,Regimen ,Oncology ,Internal medicine ,Toxicity ,medicine ,Vomiting ,medicine.symptom ,business ,medicine.drug - Abstract
From September 2001 to November 2002, 35 patients aged 70–81 (median, 75) years, with measurable metastatic lesions from colorectal carcinoma, were treated with a combination of oxaliplatin (OXA) infused i.v. over 2 h on day 1, and capecitabine, assumed orally twice a day (12-h apart) from day 2 to day 15. An alternated dose escalation for both drugs was planned over the first three cycles for each patient, in the absence of WHO grade ≥2 toxicity on previous cycle: starting doses were 85 mg/m2 for OXA, and 2000 mg/m2 (day) for capecitabine on first cycle; on second cycle, OXA was planned at 100 mg/m2, while capecitabine was planned at 2500 mg/(m2 day) on third cycle. Treatment was repeated every 3 weeks until progression, or for a maximum of 12 cycles. A total of 212 cycles were administered, with a median of 6 (range, 1–12) cycles/patient. Dose escalation was performed in 18 (51%) patients for OXA, and in 4 (11%) patients for capecitabine. No grade 4, and 10 (29%) cases of grade 3 toxicity of any type were reported. Abdominal symptoms (pain, nausea, or vomiting) affected 66% of patients, but they were of grade 3 in only 2 (6%) patients. Grade 3 diarrhoea occurred in 3 (9%) patients. Two complete and 12 partial responses (PR) were reported, for an overall response rate of 40% (95% CI, 24–58%). Progression of disease occurred in 23 (66%) patients, and 18 (51%) died. The actuarial median progression-free and survival time were 6.9 and 14.1 months, respectively.
- Published
- 2005
16. A randomised factorial trial of sequential doxorubicin and CMF vs CMF and chemotherapy alone vs chemotherapy followed by goserelin plus tamoxifen as adjuvant treatment of node-positive breast cancer
- Author
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Silvano Palazzo, G Pistillucci, Sergio Palmeri, Rossella Lauria, Vincenzo Adamo, Vito Lorusso, G. Petrella, S. De Placido, C. Pagliarulo, L. Manzione, M. De Lena, M. De Laurentiis, A. Paradiso, M. D'Aprile, Maria Giuseppa Sarobba, Gennaro Limite, Antonio Farris, F. Ferraù, R. Costanzo, Ar Bianco, DE PLACIDO S, DE LAURENTIIS M, DE LENA M, LORUSSO V, PARADISO A, DAPRILE M, PISTILLUCCI G, FARRIS A, SAROBBA MG, PALAZZO S, MANZIONE L, ADAMO V, PALMERI S, FERRAU F, LAURIA R, PAGLIARULO C, PETRELLA G, LIMITE G, COSTANZO R, and BIANCO AR
- Subjects
Adult ,Cancer Research ,medicine.medical_specialty ,Cyclophosphamide ,medicine.medical_treatment ,Urology ,Breast Neoplasms ,Disease-Free Survival ,Drug Administration Schedule ,breast cancer ,chemoendocrine treatment ,Antineoplastic Combined Chemotherapy Protocols ,Clinical Studies ,medicine ,Adjuvant therapy ,Humans ,Doxorubicin ,anthracyclines ,Gynecology ,Chemotherapy ,premenopausal ,business.industry ,Goserelin ,adjuvant therapy ,Middle Aged ,Combined Modality Therapy ,Tamoxifen ,Regimen ,Methotrexate ,Oncology ,Chemotherapy, Adjuvant ,Fluorouracil ,Lymphatic Metastasis ,Female ,business ,Follow-Up Studies ,medicine.drug - Abstract
The sequential doxorubicin → CMF (CMF = cyclophosphamide, methotrexate, fluorouracil) regimen has never been compared to CMF in a randomised trial. The role of adding goserelin and tamoxifen after chemotherapy is unclear. In all, 466 premenopausal node-positive patients were randomised to: (a) CMF × 6 cycles (CMF); (b) doxorubicin × 4 cycles followed by CMF × 6 cycles (A → CMF); (c) CMF × 6 cycles followed by goserelin plus tamoxifen × 2 years (CMF → GT); and (d) doxorubicin × 4 cycles followed by CMF × 6 cycles followed by goserelin plus tamoxifen × 2 years (A → CMF → GT). The study used a 2 × 2 factorial experimental design to assess: (1) the effect of the chemotherapy regimens (CMF vs A × CMF or arms a + c vs b + d) and (2) the effect of adding GT after chemotherapy (arms a + b vs c + d). At a median follow-up of 72 months, A → CMF as compared to CMF significantly improved disease-free survival (DFS) with a multivariate hazard ratio (HR) = 0.740 (95% confidence interval (CI): 0.556-0.986; P = 0.040) and produced a nonsignificant improvement of overall survival (OS) (HR = 0.764; 95% CI: 0.489-1.193). The addition of GT after chemotherapy significantly improved DFS (HR = 0.74; 95% CI: 0.555-0.987; P = 0.040), with a nonsignificant improvement of OS (HR = 0.84; 95% CI: 0.54-1.32). A → CMF is superior to CMF. Adding GT after chemotherapy is beneficial for premenopausal node-positive patients. © 2005 Cancer Research UK.
- Published
- 2005
17. Multicentre randomised phase III study comparing the same dose and schedule of cisplatin plus the same schedule of vinorelbine or gemcitabine in advanced non-small cell lung cancer
- Author
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Francesca Sperandi, L. Mantovani, Barbara Melotti, P. Preti, Andrea Martoni, V. Picece, F. Di Fabio, S. Giaquinta, A. Petralia, A. Marino, Monica Guaraldi, F. Artioli, G. Palomba, Antonio Farris, Martoni, A, Marino, A, Sperandi, F, Giaquinta, S, Di Fabio, F, Melotti, B, Guaraldi, M, Palomba, G, Preti, P, Petraia, A, Artioli, F, Picece, V, Farris, A, and Mantovani, L
- Subjects
Adult ,Male ,Cancer Research ,medicine.medical_specialty ,Lung Neoplasms ,medicine.drug_class ,Cost-Benefit Analysis ,Neutropenia ,Vinblastine ,NSCLC ,Vinorelbine ,Deoxycytidine ,Antimetabolite ,Gastroenterology ,Bolus (medicine) ,Carcinoma, Non-Small-Cell Lung ,Internal medicine ,Antineoplastic Combined Chemotherapy Protocols ,Humans ,Medicine ,Lung cancer ,Aged ,business.industry ,Middle Aged ,medicine.disease ,Gemcitabine ,Surgery ,Regimen ,Oncology ,Toxicity ,Disease Progression ,Female ,Cisplatin ,business ,medicine.drug - Abstract
This study compares two cytotoxic regimens comprising the same dose and schedule of cisplatin (CP) plus vinorelbine (VNR) or gemcitabine (GEM) administered under the same schedule to patients with advanced non-small cell lung cancers (NSCLC). From April 1998 to February 2003, 285 patients were randomised to receive either VNR 25 mg/m 2 on days 1 and 8 as an intravenous (i.v.) bolus plus CP 75 mg/m 2 on day 1 (regimen A) or GEM 1200 mg/m 2 on days 1 and 8 as an i.v. 30-min infusion plus CP 75 mg/m 2 on day 1 (regimen B). Both treatments were recycled every 21 days. If no progression had occurred after six cycles, the patients continued to receive VNR or GEM monochemotherapy weekly. Cross-over of the two single agents was considered if disease progression occurred. Objective response (OR), time to progression (TTP) and overall survival (OS) were analysed according to the intention-to-treat principle. 272 patients were ultimately eligible (137 on A and 135 on B). Their main characteristics were: male/female ratio 214/58; median age 63 (range 32-77) years; median Karnofsky Performance Status (PS) 80 (range 70-100); stage IIIB 34%, stage IV 61%, recurrent disease 5%; histology - epidermoid 29%, adenocarcinoma 53%, other NSCLC 18%. The characteristics of the patients in the two arms were well matched. The following response rates were observed in regimens A and B, respectively: complete response (CR) 0.7% and 3.7%, partial response (PR) 31.9% and 22.2% (P = 0.321). Median CR + PR duration was 8 months in both arms. Clinical benefit represented by an improvement in symptoms was evident in 25.7% and 28.1%, respectively. Median TTP was 5 months in both arms and median OS 11 months in both arms. Grade III-IV neutropenia occurred in 30.7% and 17.7% of the patients in arms A and B, respectively (P = 0.017); thrombocytopenia occurred in 0% and 9.3% (P = 0.004), respectively. No difference in the incidence of anaemia was observed. Non-haematological toxicity was generally mild: a higher incidence of grade 1-2 peripheral neurotoxicity and grade 1-2 local toxicity with regimen A and grade 1-2 liver toxicity with regimen B was reported. A pharmaco-economic comparison showed a difference between the two doublets, principally due to the different costs of VNR and GEM. Under the study conditions the combination of VNR or GEM with the same dose and schedule of CP produced similar OR, clinical benefits, TTP and OS in advanced NSCLC, and only mild toxicological differences were observed. Pharmaco-economic evaluation favoured the CP + VNR doublet. © 2004 Elsevier Ltd. All rights reserved.
- Published
- 2005
18. Irinotecan plus leucovorin-modulated 5-fluorouracil I.V. bolus every other week may be a suitable therapeutic option also for elderly patients with metastatic colorectal carcinoma
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L. De Lucia, Vito Lorusso, F. De Vita, S. Mancarella, Pasquale Comella, Antonio Farris, A. Gambardella, Luigi Maiorino, S. Palmeri, F. Buzzi, Comella, P, Farris, A, Lorusso, V, Palmeri, S, Maiorino, L, DE LUCIA, L, Buzzi, F, Mancarella, S, DE VITA, Ferdinando, and Gambardella, Antonio
- Subjects
Adult ,Male ,Cancer Research ,medicine.medical_specialty ,Colorectal cancer ,medicine.medical_treatment ,Leucovorin ,Adenocarcinoma ,Irinotecan ,elderly patients ,Gastroenterology ,Disease-Free Survival ,Drug Administration Schedule ,Clinical ,colorectal carcinoma ,Internal medicine ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,Carcinoma ,Humans ,5-fluorouracil ,Infusions, Intravenous ,Survival rate ,Aged ,Retrospective Studies ,Chemotherapy ,combination chemotherapy ,business.industry ,Combination chemotherapy ,Middle Aged ,medicine.disease ,Surgery ,Survival Rate ,Treatment Outcome ,Oncology ,Fluorouracil ,Camptothecin ,Female ,Colorectal Neoplasms ,business ,medicine.drug - Abstract
The aim of this study was to assess the safety and efficacy of biweekly irinotecan plus leucovorin-modulated 5-fluorouracil i.v. bolus in metastatic colorectal carcinoma according to the age of patients. For this purpose, we have analysed 108 patients randomly allocated to receive irinotecan 200 mg m(-2) i.v. (1-h infusion) on day 1, and L-leucovorin 250 mg m(-2) i.v. (1-h infusion) plus 5-fluorouracil 850 mg m(-2) i.v. bolus on day 2 every 2 weeks (IRIFAFU) in our previous SICOG 9801 trial. According to age, patients were retrospectively divided into three groups: younger (/=54 years, n=37), middle-aged (55-69 years, n=64), and elderly (/=70 years, n=17). Apart from gender, pretreatment characteristics were well balanced across the three groups. WHO grade/=3 neutropenia and diarrhoea affected on the whole 46 and 16 patients, respectively, without any significant difference according to age-grouping. Patients aged/=54 years stayed on therapy for a longer time (median 24 vs 14-15 weeks), and received more cycles (median 9 vs 7), than the older ones. Only one patient in the young group withdrew consent to therapy as opposed to four patients each in the aged and elderly one. Response rate was 38% for younger patients, 34% for aged, and 35% for the elderly ones. Median time to progression was 7.4, 8.0, and 5.3 months, and median survival time was 13.4, 15.3, and 13.9 months, respectively. We conclude that IRIFAFU given every other week may represent a suitable therapeutic option also for elderly patients with metastatic colorectal carcinoma.
- Published
- 2003
19. Interaction between Fenhexamid and Yeasts during the Alcoholic Fermentation of Saccharomyces cerevisiae
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Paolo Cabras, Maria G Fiori, Giovanni Antonio Farris, and A. Pusino
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Saccharomyces cerevisiae ,Chitin ,Alcohol ,Ethanol fermentation ,Biology ,Cell wall ,chemistry.chemical_compound ,Vitis ,Food science ,Glucans ,Glucan ,chemistry.chemical_classification ,Ethanol ,General Chemistry ,biology.organism_classification ,Amides ,Fungicides, Industrial ,Fungicide ,chemistry ,Biochemistry ,Fruit ,Fermentation ,Adsorption ,General Agricultural and Biological Sciences - Abstract
The behavior of the fungicide fenhexamid, N-(2,3-dichloro-4-hydroxyphenyl)-1-methyl-cyclohexanecarboxamide, has been studied at concentrations corresponding to the limits fixed for grapes (3 mg kg(-1)), or higher, during the alcoholic fermentation. The presence of the fungicide did not affect the amount of alcohol produced. The amount of fenhexamid in the liquid phase decreased by ca. 15%, but the missing fenhexamid was recovered unchanged from yeasts. This suggests that the fungicide is not degraded during the fermentation process, but adsorbed by yeasts. Two constituents of Saccharomyces cerevisiae cell wall, chitin and glucan, tested as potential adsorbents, exhibited affinity for fenhexamid.
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- 2003
20. DEHYDRATION PERFORMANCE OF LOCAL FIG CULTIVARS
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Antonio Piga, Mario Carlo Salvatore Agabbio, and Giovanni Antonio Farris
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Horticulture ,Non enzymatic ,Chemistry ,medicine ,Browning ,food and beverages ,Dehydration ,Cultivar ,medicine.disease - Abstract
Fig fruits dehydration is quite totally carried out by sun drying. This technology poses concern about safety of the end product, mainly for aflatoxin development. Most of the problems involved in sun drying may be solved by oven dehydration. Fig fruits of some local cultivars underwent hot air dehydration in a tangential air-flow cabinet dryer. At start, regular intervals and end of process sampling have been performed to calculate dehydration curves and quality loss. Microbiological counts, non enzymatic browning and quality parameters were also monitored during storage. Results obtained will be discussed.
- Published
- 2003
21. Tamoxifen alone versus adjuvant tamoxifen for operable breast cancer of the elderly: long-term results of the phase III randomized controlled multicenter GRETA trial
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F. Sasso, Antonio Farris, Alfonso Pluchinotta, S. Milani, Luigi Maiorino, A. De Matteis, Giorgio Mustacchi, A. Scanni, and R. Ceccherini
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Oncology ,medicine.medical_specialty ,Antineoplastic Agents, Hormonal ,medicine.medical_treatment ,Mammary gland ,Breast Neoplasms ,Disease ,Breast cancer ,Internal medicine ,medicine ,Humans ,skin and connective tissue diseases ,Aged ,Aged, 80 and over ,Chemotherapy ,Performance status ,business.industry ,Age Factors ,Hematology ,medicine.disease ,Antiestrogen ,Interim analysis ,Combined Modality Therapy ,Survival Analysis ,Surgery ,Tamoxifen ,Treatment Outcome ,medicine.anatomical_structure ,Disease Progression ,Female ,business ,hormones, hormone substitutes, and hormone antagonists ,medicine.drug - Abstract
Background To evaluate the efficacy of tamoxifen as primary treatment in women aged over 70 years with operable breast cancer versus surgery followed by adjuvant tamoxifen. Patients and methods Patients randomly received tamoxifen alone (160 mg day 1, then 20 mg/day) for 5 years or surgery followed by tamoxifen (20 mg/day) for 5 years. Overall survival was the main study end point; secondary objectives included breast cancer survival and local control of the disease. Results Between 1987 and 1992, 239 patients were assigned to surgery plus tamoxifen and 235 to tamoxifen alone. Treatment arms were comparable for tumor size, clinical nodal status and performance status. At a median follow-up of 80 months 274 patients had died. No difference between groups had emerged in overall and breast cancer survival. There were 27 local progressions in the surgery plus tamoxifen group and 106 in the tamoxifen-alone group (P = 0.0001). In the surgery plus tamoxifen group, no difference in overall survival had emerged according to the extension of operation. Conclusions The long-term results of the study confirm the 3-year interim analysis already reported. Surgery (radical or minimal) followed by adjuvant tamoxifen does not modify overall and breast cancer survival as compared with tamoxifen alone in early breast cancer of older women. Because of the high rate of local progressions with tamoxifen alone, minimal surgery followed by tamoxifen appears to be the appropriate treatment in such patients. More extensive surgery is not useful. Tamoxifen alone is an adequate alternative treatment in very old or frail patients.
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- 2003
22. Time to Progression in Metastatic Breast Cancer Patients Treated With Epirubicin Is Not Improved by the Addition of Either Cisplatin or Lonidamine: Final Results of a Phase III Study With a Factorial Design
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G. Giardina, G Moro, Luigi Dogliotti, Mario De Lena, Giorgio Bonazzi, Maria Giuseppa Sarobba, Vito Lorusso, Alberto Bottini, Federico Castiglione, S. Danese, Antonio Farris, Francesco Nuzzo, Paolo Bruzzi, Gabriella Gorzegno, Cesare Bumma, Andrea de Matteis, Alfredo Berruti, P Alquati, Raffaella Bitossi, and Enza DeFabiani
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Adult ,inorganic chemicals ,Cancer Research ,medicine.medical_specialty ,Indazoles ,medicine.medical_treatment ,Urology ,Breast Neoplasms ,Metastasis ,chemistry.chemical_compound ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,Humans ,neoplasms ,Survival analysis ,Aged ,Epirubicin ,Cisplatin ,Chemotherapy ,business.industry ,Lonidamine ,Cancer ,Middle Aged ,medicine.disease ,Survival Analysis ,Metastatic breast cancer ,Surgery ,Logistic Models ,Oncology ,chemistry ,Disease Progression ,Female ,business ,medicine.drug - Abstract
PURPOSE: To investigate the value of the addition of either cisplatin (CDDP) or lonidamine (LND) to epirubicin (EPI) in the first-line treatment of advanced breast cancer. PATIENTS AND METHODS: Three hundred seventy-one metastatic breast cancer patients with no prior systemic chemotherapy for advanced disease were randomized to receive either EPI alone (60 mg/m2 on days 1 and 2 every 21 days), EPI and CDDP (30 mg/m2 on days 1 and 2 every 21 days), EPI and LND (450 mg orally daily, given continuously), or EPI, CDDP, and LND. Time to progression, response rates, side effects, and survival were compared according to the 2 × 2 factorial design of this study. RESULTS: The groups were well balanced with respect to prognostic factors. Time to progression did not differ in the comparison between CDDP arms and non-CDDP arms (median, 10.9 months v 9.4 months, respectively; P = .10) or between that of LND arms and non-LND arms (median, 10.8 months v 9.9 months, respectively; P = .47), nor did overall survival. The response rate did not significantly differ in the comparison between LND arms and non-LND arms (62.9% v 54.0%, P = .08). No difference in treatment activity was observed between CDDP arms and non-CDDP arms. Toxicity was significantly higher in the CDDP arms, leading to CDDP dose adjustment in 40% of cases. The most frequent side effects were of a hematologic and gastrointestinal nature. The addition of LND produced more myalgias and fatigue. CONCLUSION: Neither CDDP nor LND was able to significantly improve the time to progression obtained by EPI. CDDP, however, significantly worsened the drug’s tolerability.
- Published
- 2002
23. HSP12 is essential for biofilm formation by a Sardinian wine strain ofS. cerevisiae
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Severino Zara, G. Antonio Farris, Alan T. Bakalinsky, and Marilena Budroni
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Saccharomyces cerevisiae Proteins ,Molecular Sequence Data ,Flor ,Wine ,Bioengineering ,Saccharomyces cerevisiae ,Biology ,Polymerase Chain Reaction ,Applied Microbiology and Biotechnology ,Biochemistry ,Microbiology ,Sequence Homology, Nucleic Acid ,Genetics ,DNA, Fungal ,Gene ,Alleles ,Heat-Shock Proteins ,Base Sequence ,Strain (chemistry) ,Biofilm ,Yeast ,Mutagenesis ,Biofilms ,DNA Transposable Elements ,Fermentation ,Transposon mutagenesis ,Biotechnology - Abstract
Sardinian sherry strains of S. cerevisiae form a biofilm on the surface of wine at the end of the ethanolic fermentation, when grape sugar is depleted and when further growth becomes dependent on access to oxygen. A point mutation in HSP12 or deletion of the entire gene results in inability to form this film. HSP12 encodes a heat-shock protein previously foundby others to be active during stationary phase, in cells depleted for glucose, and in cells metabolizing ethanol and fatty acids, all conditions associated with sherry biofilms. The DNA sequence of HSP12 allele of strain Ar5-H12 has GenBank Accession No. AY046957. Copyright © 2002 John Wiley & Sons, Ltd.
- Published
- 2002
24. Independent Factors Predict Supranormal CA 15-3 Serum Levels in Advanced Breast Cancer Patients at First Disease Relapse
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G Moro, Antonio Durando, E. Manzin, Marco Massobrio, Maria Giuseppa Sarobba, Antonio Farris, Giorgio Bonazzi, Alfredo Berruti, Francesco Nuzzo, Michela Donadio, Federico Castiglione, A. de Matteis, Luigi Dogliotti, Alberto Bottini, Raffaella Bitossi, E. de Fabiani, Marco Tampellini, Gabriella Gorzegno, and P. Arese
- Subjects
Adult ,Oncology ,medicine.medical_specialty ,Advanced breast ,Mammary gland ,Estrogen receptor ,CA 15-3 ,Breast Neoplasms ,Breast cancer ,Predictive Value of Tests ,Recurrence ,Internal medicine ,Biomarkers, Tumor ,medicine ,Carcinoma ,Humans ,Aged ,Aged, 80 and over ,business.industry ,Mucin-1 ,Cancer ,General Medicine ,Middle Aged ,Prognosis ,medicine.disease ,medicine.anatomical_structure ,Multivariate Analysis ,Immunology ,Female ,business ,DISEASE RELAPSE - Abstract
Data currently available are insufficient to demonstrate a real utility for CA 15-3 in the diagnosis, staging or surveillance of breast cancer patients following primary treatment. The aim of this study was to determine if there was a correlation between supranormal CA 15-3 serum levels and clinical and biological variables in breast cancer patients at first disease relapse. From October 1988 to March 1998, 430 consecutive patients entered the study. Overall CA 15-3 sensitivity was 60.7%. Elevated CA 15-3 levels were found more frequently in patients with liver metastases (74.6%) and in those with pleural effusion (75.7%). CA 15-3 sensitivity was 70.4% in patients with estrogen-receptor-positive (ER+) primary tumors and 45.9% in those with estrogen-receptor-negative (ER-) tumors (p0.0001). In patients with a limited extent of disease, marker sensitivity was 57.7% in ER+ tumors and 25.7% in ER- tumors (p0.0001). Logistic regression analysis showed ER status, disease extent and pleural effusion as independent variables associated with CA 15-3 positivity. The multivariate Cox analysis showed ER and disease extent as independent variables predicting overall survival, whereas CA 15-3 failed to be statistically significant. CA 15-3 was an independent variable only when the disease extent variable was removed. This study suggests that CA 15-3 in advanced breast cancer patients is a marker of both disease extent and ER status. The direct relationship with ER status indicates that CA 15-3 diagnostic sensitivity in the early detection of disease recurrence could be greater in ER+ patients than in ER- ones. Furthermore, this suggests that patients with elevated CA 15-3 levels could have disease that is more sensitive to hormone manipulation than those with normal CA 15-3 values.
- Published
- 2001
25. A genetic study of natural flor strains of Saccharomyces cerevisiae isolated during biological ageing from Sardinian wines
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Gérard Aimé Pinna, G. Giordano, Marilena Budroni, and Giovanni Antonio Farris
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Genetics ,Wine ,biology ,Saccharomyces cerevisiae ,Flor ,General Medicine ,Spores, Fungal ,biology.organism_classification ,Applied Microbiology and Biotechnology ,Yeast ,Microbiology ,Italy ,Ageing ,Biotechnology ,Oenology - Abstract
In this study, three flor strains of Saccharomyces cerevisiae were genetically characterized. They were isolated from biofilms on Sardinian sherry-like wines produced at family-run wineries where pure cultures of yeasts were not used. The study aimed to investigate the life cycle of these naturally-occurring flor strains, using a genetic procedure supplemented by analysis of subsequent meiotic generations. A semi-homothallic life cycle was found in three strains that could be helpful in a genetic improvement programme.
- Published
- 2000
26. Gemcitabine plus vinorelbine in advanced non-small cell lung cancer: a phase II study of three different doses
- Author
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S. Cigolari, Raffaella Felletti, Luciano Frontini, M Gulisano, M C Locatelli, F. Perrone, Federico Castiglione, Sergio Federico Robbiati, Antonio Farris, G P Ianniello, E. Piazza, Ciro Gallo, C. Gridelli, and Giampietro Gasparini
- Subjects
Adult ,Male ,Cancer Research ,medicine.medical_specialty ,Lung Neoplasms ,Neutropenia ,medicine.drug_class ,medicine.medical_treatment ,Phases of clinical research ,Vinorelbine ,Vinblastine ,Antimetabolite ,Gastroenterology ,Deoxycytidine ,Drug Administration Schedule ,Internal medicine ,Carcinoma, Non-Small-Cell Lung ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,Humans ,Lung cancer ,non-small cell lung cancer ,Aged ,Chemotherapy ,Dose-Response Relationship, Drug ,business.industry ,gemcitabine ,Regular Article ,Middle Aged ,medicine.disease ,Survival Analysis ,Gemcitabine ,Surgery ,Treatment Outcome ,Oncology ,Quality of Life ,Female ,business ,medicine.drug - Abstract
Our aim was to study the activity and toxicity of the gemcitabine plus vinorelbine (Gem Vin) combination and to identify the optimal dose. Previously untreated patients aged < 70 years, with stage IV or IIIb (not candidates for radiotherapy) non-small cell lung cancer were eligible. Studied dose-levels of Gem Vin, administered on days 1 and 8 every 3 weeks, were (mg m–2): level I = 1000/25; level II = 1200/25; level III = 1000/30; level IV = 1200/30. A feasibility study was performed at each dose-level, followed by a single-stage phase II study. Dose-level IV was unfeasible because of grade 4 neutropenia. Overall, out of 126 patients enrolled in phase II studies, there were one complete and 32 partial responses (response rate 26%: 95% CI 18–34%). Response rates were 27.9%, 21.4% and 29.3% at levels I, II and III, respectively. The treatment was well tolerated. Toxicity was less frequent and severe at level I. Overall median survival was 33 weeks (95% CI 28–40). Descriptive quality of life analysis showed that patients with a worse baseline global health status score tended to drop out of the study earlier than those with a better score. Gem Vin is feasible at different doses. It is sufficiently active and well tolerated. A phase III study to compare the effect on quality of life of Gem Vin (level I) vs cisplatin-based chemotherapy is ongoing. © 2000 Cancer Research Campaign
- Published
- 2000
27. Influence of Storage Temperature on Shelf-life of Minimally Processed Cactus Pear Fruits
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Giovanni Antonio Farris, Giuseppina Emonti, Antonio Piga, Mario Carlo Salvatore Agabbio, and Salvatore D'Aquino
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PEAR ,Horticulture ,Chemistry ,Botany ,Organoleptic ,Cactus ,Food preservation ,Cold storage ,Cultivar ,Shelf life ,Sensory analysis ,Food Science - Abstract
Cactus pear fruits ( Opuntia ficus indica Mill, cultivar Gialla) were manually peeled, placed in polystyrene trays and packaged with a heat-shrinkable film, then kept at 4 °C and 15 °C for 11 d. After 4, 8 and 11 d chemical-physical, microbiological and sensorial parameters were determined, while in-package gas concentrations were measured daily. Chemical-physical and sensorial parameters did not show significant changes in 4 °C-stored fruits until d 8, while those kept at 15 °C experienced a dramatic increase in acidity, ethanol accumulation, strong off-flavour development and loss of overall fruit freshness and firmness. Microbiological growth rate was much higher at 15 °C than at 4 °C. Visible manifestation of moulds was detected on 4 °C-stored fruits on d 11, while in the 15 °C-stored fruit, fungi colonization had already started on the fourth day. Based on these results, we conclude that quality of peeled cactus pear fruits can be maintained at 4 °C for 8 d, while abuse of temperature (15 °C) limits shelf-life to 4 d.
- Published
- 2000
28. Pesticides in Fermentative Processes of Wine
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Giovanni Antonio Farris, Gianluigi Madau, Vincenzo L. Garau, Filippo M. Pirisi, Paolo Cabras, Giuseppina Emonti, and Alberto Angioni
- Subjects
Food Handling ,Wine ,Dioxoles ,Saccharomyces cerevisiae ,Biology ,Ethanol fermentation ,Yeasts ,Lactobacillus ,Malolactic fermentation ,Leuconostoc ,Pyrroles ,Food science ,Oenococcus oeni ,food and beverages ,General Chemistry ,biology.organism_classification ,Drug Residues ,Fungicides, Industrial ,carbohydrates (lipids) ,Yeast in winemaking ,Pyrimidines ,Biochemistry ,Fermentation ,General Agricultural and Biological Sciences ,Lactobacillus plantarum - Abstract
The influence of six fungicides (azoxystrobin, cyprodinil, fludioxonil, mepanipyrim, pyrimethanil, and tetraconazole) on the fermentative activity of two yeasts (Saccharomyces cerevisiae and Kloeckeraapiculata) and two lactic bacteria (Leuconostoc oenos and Lactobacillus plantarum) was studied. The possibility of their being degraded by these yeasts and bacteria was also investigated. The presence of the pesticides did not affect alcoholic fermentation, not even with levels higher than those normally found in grapes in field experiments. On the contrary, their presence stimulated the yeast, especially K. apiculata, to produce more alcohol. The fermentative process did not affect the amount of pesticides either by degradation or by adsorption. During malolactic fermentation by Le. oenos, malic acid decreased slightly less (by approximately 15%) in the presence of all pesticides, except mepanipyrim. A lower effect ( approximately 5%) was found during the fermentative process with La. plantarum. The bacteria studied did not show a degradative effect on pesticides during malolactic fermentation.
- Published
- 1999
29. Incidence de la température et du pH sur la production d'acide malique par Saccharomyces cerevisiae
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Giovanni Antonio Farris, Fabrizio Fatichenti, and Pietrino Deiana
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Saccharomyces cerevisiae ,synthetic substrate ,anaerobic ,must ,malic acid ,Agriculture ,Botany ,QK1-989 - Abstract
Dans ce travail on étudie la formation d'acide malique par deux souches différentes de Saccharomyces cerevisiae cultivées sur substrat synthétique en conditions anaérobies, en fonction de différentes valeurs de température et pH. Les deux souches produisent environ 1 g/I d'acide malique à 25°C et à pH 4,2. L'opportunité d'utiliser ces souches pour la fermentation des moûts des régions chaudes est discutée.
- Published
- 1989
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30. THE INFLUENCE OF INDUSTRIAL DEHYDRATION ON QUALITY OF FIG FRUITS
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Salvatore D'Aquino, Antonio Piga, F. E. Sanna, Antonio Vodret, Claudio Papoff, Giangiacomo Milella, Mario Carlo Salvatore Agabbio, Giovanni Antonio Farris, Carla Sotgiu, and Gianni Battacone
- Subjects
Water activity ,Chemistry ,Blanching ,Horticulture ,Bacterial growth ,medicine.disease ,Shelf life ,Air temperature ,medicine ,Relative humidity ,Dry matter ,Food science ,Dehydration - Abstract
The effectiveness of a dehydration process on the quality of "Niedda longa" fig fruits was studied. Drying was carried out either with an industrial two-stage dehydration system, requiring no blanching treatment, or with a simulated sun-drying procedure. During the industrial process fruits were: (a) initially moved for 3 hours in a tunnel with a gradient air temperature from 95°C to 85°C; (b) then moved into a second tunnel at 85°C for 14 h. Sun-drying was simulated keeping fruits for 120 h at 32°C and 35% of relative humidity (RH). Finally dehydrated figs were rehydrated, or equilibrated with environmental RH (sun-dried figs), and both treated with potassium-sorbate solution, then film-packaged into hermetically sealed air tight bags. Moreover, half of the bags were packed in-modified atmosphere (MAP). Fruits were, then, stored at 20°C for 6 months. A 11 h dehydration time was sufficient to obtain microbially stable fruits without chemical treatments, reaching a dry matter content (DM) of 88 % and water activity (aw) of 0.60, whereas after 17 h DM and aw were 91% and 0.57, respectively. DM of industrial rehydrated figs and environmental equilibrated sun-dried figs were 71% and 74%, respectively, requiring a potassium-sorbate treatment. After packaging and 6 months of shelf life, no microbial growth was revealed on fruits. MAP resulted in no additional improvement on the microbial quality of the product. Based on these results we can state that industrial dehydration performed well, even if fruit calibration is needed before drying.
- Published
- 1998
31. Quantitative Changes of Some Volatile Components in Vernaccia di Oristano (a Sardinian Sherry-like Wine) during Maturation
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A. Carnacini, Nadia Natali, Guido C. Galletti, Andrea Antonelli, and Giovanni Antonio Farris
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Wine ,Saccharomyces cerevisiae ,Organoleptic ,Saccharomyces bayanus ,General Chemistry ,Biology ,biology.organism_classification ,Mass spectrometry ,Yeast in winemaking ,Botany ,Gas chromatography ,Food science ,General Agricultural and Biological Sciences ,Flavor - Abstract
Vernaccia di Oristano, a sherry-like wine produced in Sardinia, Italy, was subjected to biological aging after inoculation with Saccharomyces cerevisiae var. bayanus (strain 1043) and S. cerevisiae var. prostoserdovii (strain 1739) and to aging under sterile conditions. Samples were withdrawn at three different stages of maturation of the sherry-like wine, and the volatiles were analyzed by gas chromatography and gas chromatography/mass spectrometry. Forty compounds were identified comprising alcohols, acids, esters, and dioxolanes. Analysis of variance showed significant effects of both aging conditions and duration on most of the volatiles, with differences maximized at the final sampling time, i.e., 20 days after film formation. Keywords: Vernaccia di Oristano; sherry; flavor; gas chromatography/mass spectrometry; Saccharomyces cerevisiae var. bayanus; Saccharomyces cerevisiae var. prostoserdovii; yeasts
- Published
- 1997
32. Vinorelbine, cisplatin, and 5-fluorouracil as initial treatment for previously untreated, unresectable squamous cell carcinoma of the head and neck
- Author
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Antonio Farris, Vittorio Gebbia, Emilio Bajetta, Carlo Di Gregorio, Francesco Moschella, Luigi Curreli, Biagio Agostara, Glovanni Mantovani, Alberto Desogus, and Nicolo' Gebbia
- Subjects
Male ,Cancer Research ,medicine.medical_specialty ,medicine.medical_treatment ,Phases of clinical research ,Vinblastine ,Vinorelbine ,Gastroenterology ,Disease-Free Survival ,Internal medicine ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,Carcinoma ,Humans ,Aged ,Chemotherapy ,business.industry ,Induction chemotherapy ,Middle Aged ,medicine.disease ,Surgery ,Regimen ,Oncology ,Epidermoid carcinoma ,Chemotherapy, Adjuvant ,Head and Neck Neoplasms ,Fluorouracil ,Carcinoma, Squamous Cell ,Female ,Radiotherapy, Adjuvant ,Cisplatin ,business ,medicine.drug - Abstract
BACKGROUND The combination of vinorelbine (VNR), cisplatin (CDDP), and 5-fluorouracil (5-FU) has previously been shown to be active in recurrent and/or metastatic squamous cell carcinoma of the head and neck (SCHNC). This multicenter Phase II study was carried out with the aim of evaluating the effectiveness of this combination in patients with previously untreated, unresectable locally advanced SCHNC. METHODS Sixty patients with previously untreated, unresectable SCHNC were treated with CDDP 80 mg/m2 on Days 1, 5-FU 600 mg/m2 as a 4-hour infusion on Days 2-5, and VNR 25 mg/m2 iv bolus on Days 2 and 8. There were 15 patients with laryngeal carcinoma, 19 patients with oropharyngeal carcinoma, 15 with carcinoma in the oral cavity, 5 with carcinoma in the hypopharynx, and 4 with carcinoma in the maxillary sinus. Most patients (78%) had Stage IV disease. After achievement of the best possible objective response, patients were subjected to definitive locoregional treatment, i.e., radiotherapy and/or surgery, as appropriate. RESULTS All patients completed the induction chemotherapy. After a mean of 3.86 cycles per patient, the overall response rate was 88% (95% confidence interval [CI], 82-94%), with a complete response rate of 23% (95% CI, 14-26%). Complete responses were more frequently seen in patients with N0-1 disease than in those with N2-3 disease (P = 0.037). No other statistically significant correlation between type of response and extent of disease was noted. Toxicity consisted mainly of myelosuppression and gastrointestinal side effects. After definitive locoregional treatment, 58% of patients were clinically free of disease. These patients included those who had complete response after induction chemotherapy, 19 of 39 patients who had partial response, and 2 with stable disease. Median disease free survival was 16 months, and median overall survival was 23 months. CONCLUSIONS The combination regimen of CDDP, 5-FU, and VNR was very active in previously untreated SCHNC. It was well tolerated in most cases, and neurotoxicity was not a major side effect. This regimen, which does not require hospitalization, should be compared with standard chemotherapy, such as the combination of CDDP and continuous-infusion 5-FU. Cancer 1997; 79:1394-400. © 1997 American Cancer Society.
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- 1997
33. Persistence and Metabolism of Folpet in Grapes and Wine
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Elizabeth V. Minelli, Filippo M. Pirisi, Carla Sotgiu, Vincenzo L. Garau, Giovanni Antonio Farris, Paolo Cabras, Marinella Melis, and Alberto Angioni
- Subjects
Wine ,Metabolite ,food and beverages ,General Chemistry ,Ethanol fermentation ,Yeast ,Phthalimide ,chemistry.chemical_compound ,Phthalic acid ,chemistry ,Biochemistry ,Fermentation ,Food science ,General Agricultural and Biological Sciences ,Imide - Abstract
The fate of folpet from the treatment on vine to the production of wine was studied. Sunlight degraded folpet to unknown products. Phthalimide was a minor metabolite formed on grapes from folpet. Folpet degraded in must, giving 80% phthalimide; the results obtained with model solutions showed that in must folpet can also give small amounts of phthalic acid. During wine-making folpet degraded completely, and at the end of fermentation phthalimide was only present in wine. This compound was stable in wine after several months. The presence of folpet in grapes inhibited the alcoholic fermentation of Saccharomyces cerevisiae and Kloeckera apiculata completely. Phthalimide, on the contrary, had no negative effect on the fermentative action of the two yeasts. GC and HPLC methods were developed to determine folpet and its metabolites. Keywords: Folpet; metabolites; residues; grapes; wine
- Published
- 1997
34. Interaction between film-forming yeasts and phenolic acids
- Author
-
Marilena Budroni, Tonina Roggio, Vincenzo Vacca, Giovanni Antonio Farris, and Massimo Franzil
- Subjects
Wine ,Ethanol ,biology ,Saccharomyces cerevisiae ,Biofilm ,Phenolic acid ,biology.organism_classification ,Applied Microbiology and Biotechnology ,Saccharomyces ,Yeast ,Yeast in winemaking ,chemistry.chemical_compound ,chemistry ,Biochemistry ,hemic and lymphatic diseases ,Biotechnology - Abstract
Ninety strains of film-forming yeasts of the Saccharomyces genus were screened for their ability to use phenolic acids. None were able to use phenolic acids as a sole carbon source. When ethanol at 2% was added to the medium, however, the yeasts were able to grow, exhibiting florization accompanied by decolouration.
- Published
- 1997
35. Lack of Effectiveness of Adjuvant Alternating Chemotherapy in Node-Positive, Estrogen-Receptor-Negative Premenopausal Breast Cancer Patients: Results of a Multicentric Italian Study
- Author
-
Eugenio Villa, Alessandra Rubagotti, Francesco Boccardo, Paolo Pacini, Piero Sismondi, Biagio Agostara, Antonio Farris, Luigi Gallo, Domenico Amoroso, Mario Mesiti, and Alessandra Perrotta
- Subjects
Cancer Research ,medicine.medical_specialty ,Vincristine ,Chemotherapy ,Cyclophosphamide ,business.industry ,medicine.medical_treatment ,General Medicine ,Gastroenterology ,Nitrogen mustard ,Surgery ,chemistry.chemical_compound ,Oncology ,chemistry ,Fluorouracil ,Median follow-up ,Internal medicine ,medicine ,Vindesine ,business ,medicine.drug ,Epirubicin - Abstract
A multicentric randomized trial was performed in premenopausal women with node-positive, estrogen-receptor-negative breast tumors to assess the potential superiority of alternating adjuvant chemotherapy over ‘standard’ CMF chemotherapy. Between January 1989 and June 1992, 107 patients were entered into the study and randomly allocated to receive either cyclophosphamide 100 mg/m2 per os on days 1-14, methotrexate 40 mg/m2 and 5-fluorouracil 600 mg/m2 intravenously (IV) on days 1, 8 (CMF), every 4 weeks for a total of 6 cycles, or the following regimens: CMF as previously; epidoxorubicin 75 mg/m2 IV on day I and vincristine 0.75 mg/m2 IV on days 1, 8 (EV); mitomycin-C 10 mg/m2 IV on day 1 and vindesine 2 mg/m2 IV on days 1, 8 (MVs). The three regimens were given every 4 weeks for a total of 6 cycles according to the following schedule: CMF, EV, MVs, CMF, EV, MVs. At a median follow up of 48 months (range 30-72), 40 patients have relapsed and 17 have died overall. More patients in the triple-combination arm ...
- Published
- 1997
36. Pulse field gel electrophoresis study of the karyotype of some Saccharomyces cerevisiae wine strains
- Author
-
Laura Bardi, Giovanni Pinna, Tonina Roggio, Giovanni Antonio Farris, Marilena Budroni, and Carlo Nobile
- Subjects
Genetics ,Wine ,biology ,Polymorphism (computer science) ,Saccharomyces cerevisiae ,Pulsed-field gel electrophoresis ,Flor ,Karyotype ,Horticulture ,biology.organism_classification ,Food Science ,Electrophoretic karyotype - Abstract
Wine yeasts have been shown to be characterised by marked chromosome‐length polymorphism. Study of this polymorphism could explain some of the genetic characteristics of these strains and, furthermore, lead to their precise identification, which is still an unresolved problem in the farm‐produce industry. With this aim in mind, we characterised the electrophoretic karyotype of 52 flor strains, isolated from the films of the following Sardinian wines: Cannonau, Malvasia di Bosa and Vernaccia di Oristano.
- Published
- 1996
37. Volatile Composition of Vernaccia di Oristano Sherry-Like Wine as Affected by Biological Ageing
- Author
-
Guido C. Galletti, Giovanni Antonio Farris, A. Carnacini, and Andrea Antonelli
- Subjects
Wine ,Nutrition and Dietetics ,Chromatography ,biology ,Chemistry ,digestive, oral, and skin physiology ,Flavour ,food and beverages ,Flor ,biology.organism_classification ,Saccharomyces ,Yeast in winemaking ,Gas chromatography ,Gas chromatography–mass spectrometry ,Agronomy and Crop Science ,Flavor ,Food Science ,Biotechnology - Abstract
Forty compounds were identified by gas chromatography and gas chromatography/mass spectrometry in the volatile fraction of Vernaccia di Oristano, an Italian sherry-like wine produced in Sardinia since the 11th century. The individual aromatic compounds were identified and quantified in the original wine, both after biological ageing by inoculation of the original wine with Saccharomyces cerevisiaevarbayanusandS cerevisiaevarprostoserdovii, and after ageing under sterile conditions. The effects of flor yeasts in terms of production or subtraction of flavour components are discussed.
- Published
- 1996
38. Interactions during fermentation between pesticides and oenological yeasts producing H2S and SO2
- Author
-
Lorenzo Spanedda, Paolo Cabras, Vincenzo L. Garau, Marilena Budroni, Alberto Angioni, and Giovanni Antonio Farris
- Subjects
Fenthion ,Quinalphos ,General Medicine ,Biology ,biology.organism_classification ,Applied Microbiology and Biotechnology ,Saccharomyces ,Yeast ,Fenitrothion ,chemistry.chemical_compound ,Parathion ,chemistry ,Biochemistry ,Fermentation ,Food science ,Dimethoate ,Biotechnology - Abstract
The degradative action of two strains of Saccharomyces cerevisiae, producers of large quantities of H2S and SO2, on eight sulphur-containing insecticides (chlorpyrifos-methyl, dimethoate, fenitrothion, fenthion, malation, methidation, parathion, and quinalphos) was studied. Moreover, the influence of these compounds on the fermentative activity of the yeasts was investigated. The yeasts adsorbed and degraded the studied insecticides to various extents, but their fermentative activity was not affected. A moderate adsorbtion (approximately 10% of the residue) was observed for chlorpyrifos-methyl, fenitrothion, parathion, and quinalphos. When absorbed, the insecticides were also degraded by about 50%. The degraded pesticides belong to the thiophosphates, while the dithiophosphates showed higher stability. The two yeast strains showed analogous degradative actions.
- Published
- 1995
39. Clinical response after sorafenib for hepatocellular carcinoma in elderly patients: a report of two cases
- Author
-
Francesca Spada, Michela Squadroni, Katia Lorizzo, Antonio Farris, and Nicola Fazio
- Subjects
Male ,Niacinamide ,Cancer Research ,Carcinoma, Hepatocellular ,Pyridines ,Antineoplastic Agents ,03 medical and health sciences ,0302 clinical medicine ,Fatal Outcome ,Liver Cirrhosis, Alcoholic ,Humans ,Protein Kinase Inhibitors ,Aged ,Phenylurea Compounds ,Benzenesulfonates ,Liver Neoplasms ,General Medicine ,Sorafenib ,Hepatitis C ,Magnetic Resonance Imaging ,digestive system diseases ,Treatment Outcome ,Oncology ,030220 oncology & carcinogenesis ,Colonic Neoplasms ,030211 gastroenterology & hepatology ,Tomography, X-Ray Computed - Abstract
Among primary liver cancers occurring worldwide, hepatocellular carcinoma (HCC) is the major histological type accounting for 70–85% of all cases. Phase III trials in patients with advanced HCC treated with sorafenib (SO), a multitargeted tyrosine kinase inhibitor, showed significant improvements in both overall and progression-free survival. We report the cases of two elderly patients with advanced HCC who had prolonged stable disease following treatment with SO (400 mg bid). Patient 1 was treated with SO for a period of 16 months until evidence of right sacral metastases was noted. Patient 2 received SO 400 mg bid from January 2009 to October 2009 until radiological evidence of disease progression was noted. Both patients experienced minimal toxicity, suggesting that SO can be safely administered to elderly patients.
- Published
- 2012
40. Interactions between lactic bacteria and fungicides during lactic fermentation
- Author
-
Maria Teresa Melis, Mauro Meloni, P. A. Cabras, Marilena Budroni, Giovanni Antonio Farris, and T. Satta
- Subjects
biology ,Carbendazim ,Dichlofluanid ,food and beverages ,Horticulture ,biology.organism_classification ,chemistry.chemical_compound ,Triadimefon ,chemistry ,Biochemistry ,bacteria ,Leuconostoc ,Vinclozolin ,Lactic acid fermentation ,Bacteria ,Lactobacillus plantarum ,Food Science - Abstract
The influence of six fungicides (benalaxyl, carbendazim, dichlofluanid, folpet, triadimefon and vinclozolin) on the fermentative activity of two lactic bacteria (Lactobacillus plantarum and Leuconostoc oenos) was studied. The possibility of their degradation by these bacteria was also investigated. Benalaxyl, carbendazim, triadimefon and vinclozolin did not show any interaction with the bacteria studied. Dichlofluanid reduced the fermentatwe activity of L. oenos, in proportion to its concentration, while it showed no influence on the fermentative activity of L. plantarum. Dichlofluanid was rapidly degraded by L. plantarum, while its degradation by L. oenos was lower.
- Published
- 1994
41. The usefulness of Tc-99m-tetrofosmin SPECT/CT in the detection of residual tumors and axillary lymph node metastases in breast cancer patients following neoadjuvant therapy
- Author
-
Giuseppe Madeddu, Angela Spanu, Susanna Nuvoli, Daniela Sanna, Francesca Chessa, and Antonio Farris
- Subjects
Adult ,medicine.medical_specialty ,Neoplasm, Residual ,medicine.medical_treatment ,Breast surgery ,Breast Neoplasms ,Single-photon emission computed tomography ,Breast cancer ,Organophosphorus Compounds ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,Lymph node ,Neoadjuvant therapy ,Aged ,Aged, 80 and over ,Tomography, Emission-Computed, Single-Photon ,medicine.diagnostic_test ,business.industry ,Axillary Lymph Node Dissection ,General Medicine ,Organotechnetium Compounds ,Sentinel node ,Middle Aged ,medicine.disease ,Neoadjuvant Therapy ,medicine.anatomical_structure ,Lymphatic Metastasis ,Axilla ,Female ,Lymph ,Radiology ,Lymph Nodes ,business ,Tomography, X-Ray Computed - Abstract
Single photon emission computed tomography (SPECT)/CT is emerging as a useful diagnostic tool in several oncological fields. In this prospective study, we assessed the usefulness of Tc-99m-tetrofosmin SPECT/CT in the detection of both residual breast tumors and axillary lymph node metastases following neoadjuvant therapy. Thirty-seven consecutive breast cancer patients scheduled to surgery following neoadjuvant therapy preoperatively underwent a Tc-99m-tetrofosmin SPECT/CT study, using a dual head gamma camera integrated with a x-ray tube for low-dose CT, including both breasts and axillary regions in the field of view. Within 1 week of SPECT/CT, all 37 patients had breast surgery with associated axillary lymph node dissection in 33/37 cases. At surgery, 31/37 patients had breast residues (microscopic in 4/31 cases and macroscopic in 27/31 cases). Axillary lymph node metastases were ascertained in 19/33 cases (N1mi: 2 cases, N1a: 8 cases, N2a: 6 cases, N2b: 3 cases). SPECT/CT sensitivity, specificity, and accuracy in detecting residual tumors were 87%, 100%, and 89.2%, respectively; the corresponding values in detecting axillary lymph node metastases were 36.8%, 92.8%, and 60.6%. SPECT/CT missed breast cancer residues in 4/31 patients, including 2 cases with microscopic residual disease. Moreover, lymph node metastases were missed in 12/19 patients (10/12 with pN1mi or pN1a metastases), all with lymph nodes with post-therapy fibrotic changes and small deposits of metastases. Tc-99m-tetrofosmin SPECT/CT proved a useful diagnostic tool in the detection and in the localization of residual breast tumors following neoadjuvant therapy. The procedure lacked in sensitivity in identifying axillary lymph node metastases, especially in patients with a limited lymph node involvement. According to our data, SPECT/CT may guide the surgeon to the most appropriate breast surgical treatment and to eventually select the most suitable axillary lymph node sampling (axillary lymph node dissection or sentinel node biopsy).
- Published
- 2011
42. Influence of fenamidone, indoxacarb, pyraclostrobin, and deltamethrin on the population of natural yeast microflora during winemaking of two sardinian grape cultivars
- Author
-
Severino, Zara, Pierluigi, Caboni, Davide, Orro, Giovanni Antonio, Farris, Filippo, Pirisi, and Alberto, Angioni
- Subjects
Food Handling ,Pesticide Residues ,Food Contamination ,Wine ,Strobilurins ,Italy ,Yeasts ,Fermentation ,Nitriles ,Oxazines ,Pyrethrins ,Food Microbiology ,Pyrazoles ,Vitis ,Carbamates ,Imidazolines ,Candida - Abstract
The influence of fenamidone ((S)-1-anilino-4-methyl-2-methylthio-4-phenylimidazolin-5-one), pyraclostrobin (methyl 2-[1-(4-chlorophenyl)pyrazol-3-yloxymethyl]-N-methoxycarbanilate), indoxacarb (methyl 7-Chloro-2,5-dihydro-2-[[(methoxycarbonyl) [4- (trifluoromethoxy) phenyl] amino] carbonyl] indeno[1,2-e][1,3,4] oxadiazine-4a(3H)-carboxylate), and deltamethrin ([cyano-[3-(phenoxy)phenyl]methyl] 3-(2,2-dibromoethenyl)-2,2-dimethylcyclopropane-1-carboxylate) on spontaneous fermentation carried out by natural yeast grapes microflora, was studied during the wine-making process. Aliquots of pesticide standard solutions were added to the grapes before crushing, to reach a concentration equal or half the maximum residue limit (MRL). Vinifications were performed, with maceration (R), or without maceration (W). During the wine-making process, samples were taken at the beginning (one hour after grapes crushing), at the middle and at the end of the spontaneous fermentation process. At half the MRL concentration, deltamethrin affected Pichia sp. population with a decrease of almost 50 %, while fenamidone decreased Candida sp., Candida stellata at 83, and 36%, respectively. Metschnikowia pulcherrima population decreased in all samples when compared to the control. Experiments at MRL levels showed a strong reduction for all non-Saccharomyces yeast species, when grapes had been treated with pyraclostrobin, fenamidone, and deltamethrine, except for Candida sp. which was found to have been affected only by fenamidone residues. Growth zone inhibition test showed only an in vitro activity of pyraclostrobin over Kloeckera spp., C. stellata, and M. pulcherrima. Microvinification experiments produced wines with no differences concerning S. cerevisiae population as well as production of ethanol and residual sugars. Experiments showed that at the end of the fermentation process pesticides were adsorbed by the lees and grape skins, and no pesticides residue was detectable in wine.
- Published
- 2011
43. Cellular fatty acid composition in film-forming strains of two physiological races of Saccharomyces cerevisiae
- Author
-
Marilena Budroni, Maria Elisabetta Guerzoni, Milena Sinigaglia, and Giovanni Antonio Farris
- Subjects
chemistry.chemical_classification ,Degree of unsaturation ,Strain (chemistry) ,Cell ,Saccharomyces cerevisiae ,Fatty acid ,Biology ,biology.organism_classification ,Applied Microbiology and Biotechnology ,Yeast ,medicine.anatomical_structure ,chemistry ,Biochemistry ,medicine ,Fatty acid composition ,Chemical composition - Abstract
Eleven strains belonging to two physiological races of Saccharomyces cerevisiae endowed with different abilities of forming films at air-liquid interfaces were analysed in relation to cell fatty acid composition and cell hydrophobicity. Extensive individual differences in fatty acid profiles were observed both in the film and in the non-film phase. The ability of the cells to form a floating film seems to be an implicit strain character associated with an elevated unsaturation level and a mean chain length of fatty acid residues, as well as cellular hydrophobicities higher than those shown by non-film-forming strains belonging to the same species.
- Published
- 1993
44. Cisplatin-VP16 Alternating with Cyclophosphamide-Epirubicin versus Cyclophosphamide-Epirubicin-Vincristine in Small Cell Lung Cancer
- Author
-
T. Scotto, G. Sanna, S. Valzelli, M.G. Sarobba, Antonio Farris, C. Intini, and M. Bisail
- Subjects
Adult ,Male ,0301 basic medicine ,Oncology ,Vincristine ,medicine.medical_specialty ,Lung Neoplasms ,Cyclophosphamide ,medicine.medical_treatment ,030106 microbiology ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,Humans ,Pharmacology (medical) ,Carcinoma, Small Cell ,Survival rate ,Etoposide ,Aged ,Epirubicin ,Pharmacology ,Cisplatin ,Chemotherapy ,business.industry ,Respiratory disease ,Middle Aged ,medicine.disease ,Surgery ,Survival Rate ,Infectious Diseases ,030220 oncology & carcinogenesis ,Female ,business ,medicine.drug - Abstract
In the hope of increasing the incidence of objective remissions and the survival time of patients with small cell lung cancer, we conducted a randomized study designed to compare a treatment scheme of alternating chemotherapy featuring cisplatin+etoposide followed by cyclophosphamide+epirubicin versus conventional chemotherapy with cyclophosphamide+epirubicin+vincristine, in a total of 113 patients (56 treated with the alternating regime and 57 treated conventionally). Patients receiving the alternating drug regimen showed some increase in objective remission rates, and above all increased mean survival time (297 days versus 232). The higher incidence of side effects encountered was effectively controlled by the usual medical therapy.
- Published
- 1993
45. Rationale for treatment and study design of tailor: a randomized phase III trial of second-line erlotinib versus docetaxel in the treatment of patients affected by advanced non-small-cell lung cancer with the absence of epidermal growth factor receptor mutations
- Author
-
Roberto Labianca, Alessandro Bertolini, Flavia Longo, Eliana Rulli, Andrea Mancuso, Olga Martelli, M. Tomirotti, Maria Cristina Locatelli, Daniele Fagnani, Ida Pavese, Enrico Cortesi, Antonio Farris, A. Scanni, Giuseppe Valmadre, Gabriella Farina, Antonio Ardizzoia, Luca Moscetti, and Marina Chiara Garassino
- Subjects
Pulmonary and Respiratory Medicine ,Oncology ,Cancer Research ,medicine.medical_specialty ,Lung Neoplasms ,Antineoplastic Agents ,Docetaxel ,Pharmacology ,Disease-Free Survival ,Erlotinib Hydrochloride ,Gefitinib ,Internal medicine ,Carcinoma, Non-Small-Cell Lung ,Clinical endpoint ,Biomarkers, Tumor ,Medicine ,Humans ,Epidermal growth factor receptor ,Lung cancer ,Survival rate ,Neoplasm Staging ,biology ,business.industry ,medicine.disease ,Prognosis ,respiratory tract diseases ,ErbB Receptors ,Survival Rate ,Genes, ras ,Treatment Outcome ,Research Design ,Mutation ,biology.protein ,Quality of Life ,Quinazolines ,Taxoids ,Erlotinib ,business ,medicine.drug ,Follow-Up Studies - Abstract
We present the rationale and study design of the Tarceva Italian Lung Optimization trial phase III, multicenter, open-label, randomized trial on efficacy of second-line therapies in different subgroups of non-small-cell lung cancer (NSCLC) patients identified using molecular and clinical evaluations. To date, we can assume that advanced NSCLC epidermal growth factor receptor (EGFR)-mutated patients benefit from EGFR tyrosine kinase inhibitors, such as gefitinib and erlotinib, whereas their role in the treatment of patients who do not have EGFR mutations is controversial. The aim of this study is to assess whether it is possible to optimize second-line treatment in NSCLC patients with absence of EGFR mutations. Moreover, the predictive value of the K-ras mutation, EGFR protein expression, and EGFR gene copy number, as well as a smoking habit and histotype for determining a different effect of erlotinib compared with chemotherapy will be assessed in patients who do not have EGFR mutations. The primary endpoint is overall survival; the secondary endpoints are progression-free survival, response rate, quality of life, and toxicity. We have planned to collect blood samples to identify different prognosis-related polymorphisms and to assess their sensitivity and specificity in the detection of EGFR and K-ras mutations with respect to histologic samples.
- Published
- 2010
46. Epirubicin followed by cyclophosphamide, methotrexate and 5-fluorouracil versus paclitaxel followed by epirubicin and vinorelbine in patients with high-risk operable breast cancer
- Author
-
Marina Faedi, Alessandra Rubagotti, Dino Amadori, Angelo Gambi, Piero Sismondi, Biagio Agostara, Francesco Boccardo, Pamela Guglielmini, Antonio Farris, and Giuseppina Catalano
- Subjects
Oncology ,Adult ,Cancer Research ,medicine.medical_specialty ,Cyclophosphamide ,Paclitaxel ,medicine.medical_treatment ,Breast Neoplasms ,Pharmacology ,Vinorelbine ,Vinblastine ,Disease-Free Survival ,Drug Administration Schedule ,Breast cancer ,Internal medicine ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,Humans ,Aged ,Epirubicin ,Chemotherapy ,business.industry ,Cancer ,General Medicine ,Middle Aged ,medicine.disease ,Methotrexate ,Treatment Outcome ,Fluorouracil ,Multivariate Analysis ,Female ,Breast disease ,business ,medicine.drug - Abstract
Objective: Breast cancer patients with >3 involved nodes (N+) have a poor outcome. Chemotherapy (CT), alone or combined with endocrine therapy (ET) in hormone receptor (HOR)-positive patients, is the standard for these women. However, there are still questions surrounding the optimal adjuvant CT regimen. Methods: 244 patients with >3 N+ were randomized to receive either four 3-weekly courses of epirubicin (E: 100 mg/m2, day 1) followed by four 4-weekly cycles of cyclophosphamide, methotrexate and 5-fluorouracil (CMF: 600, 40, 600 mg/m2, days 1, 8: n = 122) or four 3-weekly courses of paclitaxel (T: 175 mg/m2, day 1) followed by four 3-weekly cycles of epirubicin and vinorelbine (E: 75 mg/m2, day 1; V: 25 mg/m2, days 1, 8: n = 122). After CT, tamoxifen (plus an LH-RH analog in menstruating women) was given to all HOR-positive patients over a period of 5 years. Overall survival (OS) was the primary end point. Relapse-free survival (RFS) and toxicity were secondary end points. Results: At a median follow-up time of 102 months (range 3–146), OS and RFS did not differ significantly between groups (E-CMF vs. T-EV: OS, HR 0.94, 95% CI 0.59–1.48, p = 0.8; RFS, HR 0.86, 95% CI 0.57–1.29, p = 0.45). The lack of any difference between assigned treatments was confirmed by multivariate analysis (E-CMF vs. T-EV: RFS, HR 0.98, 95% CI 0.64–1.48, p = 0.9). The 2 regimens showed different toxicity profiles. In fact, significantly more women assigned to E-CMF were affected by stomatitis (p = 0.001) while significantly more women in the T-EV group developed peripheral neuropathy (p < 0.0001) and musculoskeletal disorders (p < 0.0001). However, side effects were moderate and manageable and no toxic death occurred in either arm of the study. Conclusions: T-EV was safe and moderately toxic but was not superior to E-CMF.
- Published
- 2010
47. A genetically improved wine yeast
- Author
-
Pietrino Deiana, Giovanni Antonio Farris, L. Bifulco, Enrico Berardi, T. Satta, and Fabrizio Fatichenti
- Subjects
Genetics ,Cell fusion ,biology ,Strain (biology) ,Auxotrophy ,Saccharomyces cerevisiae ,Mutant ,Bioengineering ,Uracil ,General Medicine ,Protoplast ,biology.organism_classification ,Applied Microbiology and Biotechnology ,chemistry.chemical_compound ,Yeast in winemaking ,chemistry ,Biotechnology - Abstract
Most of 31 hybrids, obtained by fusion between the petite mutant of a nonsporous strain ofSaccharomyces cerevisiae SS-1090 and a Kar mutant K1 killer ofS. cerevisiae adenine and uracil auxotrophic, proved to be enologically as useful as the parent strain, and in some cases more so. In addition, all of them possessed killer factor, rendering them potentially more competitive against wild yeasts. Most of them also proved to be highly sporous.
- Published
- 1992
48. A role of BRCA1 and BRCA2germline mutations in breast cancer susceptibility within Sardinian population
- Author
-
Sandra Orrù, Gennaro Landriscina, Grazia Palomba, Laura Crisponi, Maria Monne, Mario Lovicu, Mario Budroni, Maria Cristina Santona, Antonio Farris, Antonio Cossu, Angela Loi, Antonella Uras, Antonio Contu, Giovanna Piras, Giuseppe Palmieri, Marina Pisano, Francesco Tanda, Patrizia Fancello, Attilio Gabbas, and Carlo Floris
- Subjects
Cancer Research ,endocrine system diseases ,Genes, BRCA2 ,Genes, BRCA1 ,Breast Neoplasms ,medicine.disease_cause ,lcsh:RC254-282 ,DNA sequencing ,Cohort Studies ,Breast cancer ,Germline mutation ,Recurrence ,Surgical oncology ,Ovarian carcinoma ,Genetics ,Humans ,Medicine ,Genetic Predisposition to Disease ,skin and connective tissue diseases ,Gene ,Germ-Line Mutation ,Aged ,Family Health ,Ovarian Neoplasms ,Mutation ,business.industry ,Middle Aged ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,medicine.disease ,Phenotype ,Italy ,Oncology ,Female ,business ,Research Article - Abstract
Background In recent years, numerous studies have assessed the prevalence of germline mutations in BRCA1 and BRCA2 genes in various cohorts. We here extensively investigated the prevalence and geographical distribution of BRCA1-2 mutations in the entire genetically-homogeneous Sardinian population. The occurrence of phenotypic characteristics which may be predictive for the presence of BRCA1-2 germline mutations was also evaluated. Methods Three hundred and forty-eight breast cancer patients presenting a familial recurrence of invasive breast or ovarian carcinoma with at least two affected family members were screened for BRCA1-2 mutations by DHPLC analysis and DNA sequencing. Association of BRCA1 and BRCA2 mutational status with clinical and pathological parameters was evaluated by Pearson's Chi-Squared test. Results and Conclusion Overall, 8 BRCA1 and 5 BRCA2 deleterious mutations were detected in 35/348 (10%) families; majority (23/35;66%) of mutations was found in BRCA2 gene. The geographical distribution of BRCA1-2 mutations was related to three specific large areas of Sardinia, reflecting its ancient history: a) the Northern area, linguistically different from the rest of the island (where a BRCA2 c.8764_8765delAG mutation with founder effect was predominant); b) the Middle area, land of the ancient Sardinian population (where BRCA2 mutations are still more common than BRCA1 mutations); and c) the South-Western area, with many Phoenician and Carthaginian locations (where BRCA1 mutations are prevalent). We also found that phenotypic features such as high tumor grading and lack of expression of estrogen/progesterone receptors together with age at diagnosis and presence of ovarian cancer in the family may be predictive for the presence of BRCA1-2 germline mutations.
- Published
- 2009
49. Neo-adjuvant exemestane in elderly patients with breast cancer: a phase II, multicentre, open-label, Italian study
- Author
-
Sandro Barni, C. Dellach, Giorgio Mustacchi, Mauro Mansutti, Cosimo Sacco, M. Cozzi, Marina Cazzaniga, Antonio Farris, Mustacchi, G, Mansutti, M, Sacco, C, Barni, S, Farris, A, Cazzaniga, M, Cozzi, M, Dellach, C, Mustacchi, Giorgio, and Dellach, C.
- Subjects
Endocrine therapy ,medicine.medical_specialty ,medicine.drug_class ,medicine.medical_treatment ,Antineoplastic Agents ,Breast Neoplasms ,Neo-adjuvant ,Exemestane ,chemistry.chemical_compound ,Breast cancer ,breast cancer ,Internal medicine ,Clinical endpoint ,Breast-conserving surgery ,Medicine ,Humans ,Adverse effect ,Aged ,Aged, 80 and over ,Aromatase inhibitor ,business.industry ,neoadjuvant endocrine ,Cancer ,Hematology ,medicine.disease ,Combined Modality Therapy ,Surgery ,Androstadienes ,Treatment Outcome ,Oncology ,chemistry ,Chemotherapy, Adjuvant ,Female ,Breast disease ,business - Abstract
Background: The steroidal aromatase inhibitor exemestane has demonstrated efficacy for the treatment of breast cancer in the metastatic and adjuvant settings. Smaller trials have also reported efficacy in the neo-adjuvant setting. Patients and methods: This phase II, open-label, multicentre study examined the efficacy and safety of neo- adjuvant exemestane in women aged >70 years with operable, receptor-rich breast cancer. Consecutive eligible patients received exemestane 25 mg/day for 6 months before planned surgery. The primary end point was clinical response. Results: Overall, 117 patients were recruited (median age 80 years). The objective response rate in 112 assessable patients (85 with clinical and mammographic evaluation; 27 with clinical evaluation only) was 69.6% (two complete responses; 76 partial responses). In patients who responded, median tumour size reduced from 4.81 to 2.12 cm. Seventy-seven patients (68.7%) continued to surgery. Of the 40 patients eligible for breast-conserving surgery, 34 (85%) deemed unfit for this procedure at baseline. Exemestane-related adverse events were unremarkable except for grade 3 allergic skin reactions in two patients (1.8%). Conclusion: Neo-adjuvant exemestane given for 6 months appears to be effective for receptor-rich breast cancer in older patients. There may now be sufficient evidence to support the use of neo-adjuvant in this patient population. Key words: aromatase inhibitor, breast cancer, endocrine therapy, exemestane, neo-adjuvant
- Published
- 2009
50. Fenhexamid adsorption behavior on soil amended with wine lees
- Author
-
Alba Pusino, Maria Vittoria Pinna, Giovanni Antonio Farris, and Marilena Budroni
- Subjects
Fermentation in winemaking ,Wine ,Chromatography ,Chemistry ,Amendment ,food and beverages ,Industrial Waste ,General Chemistry ,Lees ,Amides ,Fungicides, Industrial ,Soil ,Adsorption ,Environmental chemistry ,Yeasts ,Soil water ,Dissolved organic carbon ,Fermentation ,General Agricultural and Biological Sciences - Abstract
The adsorption of fenhexamid (FEN) [N-(2,3-dichloro-4-hydroxyphenyl)-1-methylcyclohexanecarboxamide] on vineyard soil amended with wine lees (WL) produced by vinery was studied. The adsorption extent depends on WL fraction. The addition of the centrifuged solid lees (SWL) increases the FEN adsorption on soil. Most likely, the organic insoluble fraction formed mainly by dead fermentation yeasts is responsible for the observed increase. The adsorption measured on some deactivated yeasts of wine fermentation shows that Saccharomyces cerevisiae are the most active in FEN retention. On the other hand, the soil amendment with whole WL decreases considerably the fungicide adsorption. This opposite effect may be the result of FEN hydrophobic bonds with the dissolved organic matter of lees that keeps fungicide in solution. This hypothesis is substantiated by the increased FEN solubility in the supernatant of centrifuged wine lees (LWL). The results of soil column mobility confirm that the elution with LWL increases the mobility of FEN in soil.
- Published
- 2008
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