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Background/Objectives: conducting fitness tests in educational settings can lead to advantages for both individuals and groups, beyond just enhancing fitness. With the aim of appropriately interpreting performance, this study was conducted to establish sex- and age-specific percentile normative data for a physical fitness (PF) protocol and to define a compound measure of physical performance. Methods: In this cross-sectional study, a short, feasible, and easy-to-administer test battery was performed for 5106 school children, aged 6–10 years. Gender- and age-specific percentiles for the PF tests were constructed, and a Physical Fitness–Motor Competence Performance Index (PF-MC PI) was calculated according to the percentiles. Results: Boys scored higher in all the PF tests when compared to girls (p < 0.001). There was also a trend towards increased PF levels as the age increased in both genders (p < 0.0001). Correlations between scores on individual test items were moderate to high (r from 0.549 to 0.700), and all individual test item scores significantly correlated with the PF-MC PI (p < 0.0001). Conclusions: the provided percentile values will enable the correct interpretation and monitoring of the fitness status of children and the PF-MC PI can be used for easily rating children’s health-related fitness qualities.

The phenomenon of doping is a public health issue that poses threats to sport and society. In recent decades, the emphasis on efforts to address the issue and reduce the incidence of doping by young people in sport has shifted from deterrence through testing and punishment to the promotion of clean sport behaviours through values-based education. The “I Run Clean project” sought to develop new and effective tools targeting grassroots athletes and those around them (coaches, medical support personnel, sport leaders, parents). These included sport-specific e-learning and in-person peer-to-peer workshops led by trained volunteer ambassadors. The aim of all “I Run Clean” measures is to go beyond the warnings and provision of factual information about early anti-doping campaigns to a more holistic educational approach that focuses participants on their personal and sport-related values in order to encourage good decision-making and resistance to doping-related behaviours. This study evaluates the efficacy of the peer-to-peer workshops and their impact on selected psycho-social variables. The collaboration of the volunteer ambassadors is shown to effectively transmit the desired reasoning, reduce doping risk factors and enhance protective factors.

Background: A training program can stimulate physiological, anatomical, and performance adaptations, but these improvements can be partially or entirely reversed due to the cessation of habitual physical activity resulting from illness, injury, or other influencing factors.Purpose: To investigate the effects of detraining on cardiorespiratory, metabolic, hormonal, muscular adaptations, as well as short-term and long-term performance changes in endurance athletes.Methods: Eligible studies were sourced from databases and the library up until July 2023. Included studies considered endurance athletes as subjects and reported on detraining duration.Results: Total cessation of training leads to a decrease in VO2max due to reductions in both blood and plasma volume. Cardiac changes include decreases in left ventricular mass, size, and thickness, along with an increase in heart rate and blood pressure, ultimately resulting in reduced cardiac output and impaired performance. Metabolically, there are declines in lactate threshold and muscle glycogen, increased body weight, altered respiratory exchange ratio, and changes in power parameters. In the short term, there is a decrease in insulin sensitivity, while glucagon, growth hormone, and cortisol levels remain unchanged. Skeletal muscle experiences reductions in arterial-venous oxygen difference and glucose transporter-4. Implementing a partial reduction in training may help mitigate drastic losses in physiological and performance parameters, a consideration when transitioning between training seasons.Conclusion: There is a dearth of data investigating the detraining effects of training reduction/cessation among endurance athletes. Delving deeper into this topic may be useful for professionals and researchers to identify the optimal strategies to minimize these effects.

The Covid-19 outbreak forced many governments to enter a nationwide lockdown. The aim of this study was to evaluate, by means of a survey, changes in sleep parameters and physical activity characteristics of elite track and field athletes in three periods: before the lockdown (T0), during the lockdown (09th March – 03rd May 2020, T1) and the first month after the lockdown (T2). This study was conducted from May 2020 to June 2020 and data were collected using an offline survey with 89 elite track and field athletes (mean age: 24.7±5.4; n = 43 males; n = 46 females). The survey consisted of demographic data and questions on physical activity and sleep behavior at T0, T1 and T2. Athletes reported lower sleep quality scores at T1 compared to T0 and T2 (p < 0.0001) and registered delayed bedtime, wake-up time and longer sleep latency during the lockdown compared to pre-lockdown and post-lockdown whereas no changes in total sleep time were reported. No inter-group differences were detected in sleep characteristics between short- and long-term disciplines and between genders. The weekly training volume decreased from 16.1±5.7 hours at T0 to 10.7±5.7 hours at T1 (p < 0.0001) whereas no significant differences were detected in training volume during the lockdown in relation to the square footage of the house (p = 0.309). Alcohol (p = 0.136) and caffeine intake (p = 0.990) and use of electronic devices (p = 0.317) were similar pre-, during, and post-lockdown. The unprecedented circumstances of the Covid-19 pandemic had negative impacts on the Italian track and field athletes’ sleep and training volumes.

This is a critical review of the current literature data about sexual dysfunction as a potential side effect related to drugs commonly used for the treatment of Benign Prostatic Hyperplasia and Lower Urinary Tract Symptoms. In this narrative review, we analyzed data from the literature related to the development of sexual dysfunctions during the treatment of BPH or LUTS. Both α-blockers and 5-alpha reductase inhibitors (5-ARIs) can induce erectile dysfunction, ejaculatory disorders and a reduction in sexual desire. The sexual side effect profile of these drugs is different. Among the α-blockers, silodosin appears to have the highest incidence of ejaculatory disorders. Persistent sexual side effects after the discontinuation of finasteride have been recently reported; however, further studies are needed to clarify the true incidence and the significance of this finding. However, most of the published studies are affected by a weak methodology and other important limitations, with only a few RCTs available. Therefore, it is desirable that future studies will include validated tools to assess and diagnose the sexual dysfunction induced by these medications, especially for ejaculation and sexual desire disorders.

Understanding the game demands encountered in basketball provides useful insight for developing specific, individualized and team-based training sessions. This study quantified and compared the game activity demands encountered by basketball players of different playing positions: i) strictly when in possession of the ball and ii) overall during live playing time (irrespective of ball possession). The activity demands encountered by 44 (22 guards, 14 forwards, 8 centres) adult, professional, male basketball players were assessed across 10 official games. Time-motion analysis was used to determine the frequency and proportion (%) of playing time performing recovery (REC), low- (LIA), moderate- (MIA), and high- (HIA) intensity activities. Linear mixed models were constructed to examine differences in dependent variables between playing positions, accounting for repeated measures. Guards, forwards, and centres spent 11.9±5.9%, 3.5±1.3%, and 2.9±1.1% of live playing time in possession of the ball, respectively. Guards performed more activities at all intensities (total movements, REC, LIA, MIA, and HIA) than forwards (P < 0.05) and centres (P < 0.05) when in possession of the ball. The proportion of time spent performing HIA in possession of the ball was greater for forwards (P = 0.001) and centres (P = 0.001) than guards. During live playing time overall across games, centres performed more HIA per minute (P = 0.049) and spent a greater proportion of time performing HIA (P = 0.047) than guards. Activities performed when in possession of the ball and during live playing time across basketball games are affected by playing position. These data highlight the need to develop position-specific training drills, particularly with ball possession.

Load monitoring in basketball is fundamental to develop training programs, maximizing performance while reducing injury risk. However, information regarding the load associated with specific activity patterns during competition is limited. This study aimed at assessing the external load associated with high-intensity activities recorded during official basketball games, with respect to different (1) activity patterns, (2) playing positions, and (3) activities performed with or without ball. Eleven male basketball players (six backcourt, five frontcourt, age: 20.5 ± 1.1 years, stature: 191.5 ± 8.7 cm, body mass: 86.5 ± 11.3 kg; experience: 8.5 ± 2.4 years) competing in the Lithuanian third division were recruited for this study. Three in-season games were assessed via time-motion analysis and microsensors. Specifically, the high-intensity activities including sprints, high-intensity specific movements (HSM) and jumps were identified and subsequently the external load [PlayerLoad™ (PL) and PlayerLoad™/min (PL/min)] of each activity was determined. Linear mixed models were used to examine differences in PL, PL/min and mean duration between activity pattern, playing positions and activities performed with or without ball. Results revealed PL was lower in jumps compared to sprints [p < 0.001, effect size (ES) = 0.68] and HSMs (p < 0.001, ES = 0.58), while PL/min was greater in sprints compared to jumps (p = 0.023, ES = 0.22). Jumps displayed shorter duration compared to sprints (p < 0.001, ES = 1.10) and HSMs (p < 0.001, ES = 0.81), with HSMs lasting longer than sprints (p = 0.002, ES = 0.17). Jumps duration was longer in backcourt than frontcourt players (p < 0.001, ES = 0.33). When considering activity patterns combined, PL (p < 0.001, ES = 0.28) and duration (p < 0.001, ES = 0.43) were greater without ball. Regarding HSMs, PL/min was higher with ball (p = 0.036, ES = 0.14), while duration was longer without ball (p < 0.001, ES = 0.34). The current findings suggest that external load differences in high-intensity activities exist among activity patterns and between activities performed with and without ball, while no differences were found between playing positions. Practitioners should consider these differences when designing training sessions.

Italy is one of the first European epicenters of the COVID-19 pandemic. In attempts to hinder the spread of the novel coronavirus disease, Italian government hardened protective measures, from quarantine to lockdown, impacting millions of lives dramatically. Amongst the enacted restrictions, all non-essential activities were prohibited as well as all outdoor activities banned. However, at the first spur of the outbreak, for about a dozen of days, physical and sports activities were permitted, while maintaining social distancing. In this timeframe, by administering measures coming from self-determination theory and theory of planned behavior and anxiety state, in an integrated approach, we investigated the prevalence of these activities by testing, via a Structural Equation Model, the influence of such psychosocial variables on the intention to preserve physical fitness during the healthcare emergency. Through an adequate fit of the hypothesized model and a multi-group analysis, we compared the most COVID-19 hit Italian region – Lombardy – to the rest of Italy, finding that anxiety was significantly higher in the Lombardy region than the rest of the country. In addition, anxiety negatively influenced the intention to do physical activity. Giving the potential deleterious effects of physical inactivity due to personal restrictions, these data may increase preparedness of public health measures and attractiveness of recommendations, including on the beneficial effects of exercise, under circumstances of social distancing to control an outbreak of a novel infectious disease.

Background Perceived school self-efficacy (SE) is an important variable in students’ activities as it affects their motivation and learning. Further, self-efficacy might represent a good predictor of performance, persistence and perseverance. Motor skills and other physical health determinants are extensively debated and linked to cognitive function in children of developmental age. However, inconclusive evidence supports a definitive relationship between perceived school SE and motor skills among schoolchildren. We conducted a cross-sectional study on 6–11-year-old schoolchildren to evaluate the extent by which perceived school SE and physical health determinants were related. Methods A SE questionnaire and motor performance battery tests were administered to primary school pupils recruited from 154 sampled schools of northwest Italy. Perceived SE at school was assessed via 12 items from the Caprara’s questionnaire. Motor performance scores were obtained from motor skill tests: 4 × 10 m shuttle run test, SRT; standing broad jump, SBJ; six-minute walking test, 6MWT. Results A total of 3,962 children (M = 2,019; F = 1943) were studied and 68% were normal weight. Overall, a 58% of the sample perceived a high SE, while, as to gender differences, a greater percentage of females perceived high levels of school SE with respect to any other level (χ2 = 38.93, p < 0.0001). Results from multinomial logistic regression analysis revealed that: (i) females perceived higher SE compared to males; (ii) children who performed better in SRT and 6MWT showed higher levels of perceived school SE; (iii) no significant effect was registered for the body weight. Alternative strategies are encouraged to enhance SE through physical education: structured interventions might enhance both complex motor skills and high-order cognitive skills, like SE, in young children.

PURPOSE:The aim of this study was to quantify changes in physical capacities of thirty-eight basketball players selected from different teams, as well as from varying competitive levels (i.e. Division I, Division II and Division III) during the preparation and in-season periods. METHODS:Pre (T1) and post (T2) preparation period and during regular season (T3), the players completed a Yo-Yo Intermittent Recovery test-level 1. Following a 3 to 8 days-break, players performed a 6-min continuous running test (Mognoni's test), a counter-movement jump test and a 5-min high-intensity intermittent running test. RESULTS:Blood lactate concentration measured after the Mognoni's test was significantly reduced from T1 to T2, and from T2 to T3 (P

La capacità ideo-motoria fa riferimento alla possibilità che ha l’uomo di visualizzare e analizzare l’andamento spazio-temporale di persone/oggetti. In ambito sportivo il gesto che esalta questa capacità è la valutazione della traiettoria durante un lancio che viene stimata nella sua efficienza con il tempo di reazione fra la comparsa dello stimolo e l’inizio della risposta. Scopo dello studio è stato quello di valutare se due diversi approcci metodologici abbiano lo stesso effetto sul tempo di reazione. In particolare, quattro classi di una scuola media sono state divise in due gruppi di lavoro, dei quali uno ha eseguito esercitazioni sottoposte ad un controllo motorio a circuito chiuso (CC), mentre l’altro ad un controllo motorio a circuito aperto (CA). In entrambi i gruppi di lavoro le esercitazioni si sono realizzate sotto l’aspetto di un transfer motorio poiché le attività motorie svolte non possedevano le stesse caratteristiche del gesto finale che è stato analizzato. Entrambe le vie sperimentali hanno portato a miglioramenti significativi.

Il Cooperative Learning è un protocollo didattico in cui gli studenti lavorano insieme in piccoli gruppi per raggiungere obiettivi comuni, migliorare reciprocamente le loro abilità e quindi competenze. Tale metodo si distingue sia dall’apprendimento competitivo che dall’apprendimento individualistico e si presta ad essere applicato ad ogni compito, materia e curricolo. Nei team di lavoro della didattica cooperativa, gli studenti si dedicano all’attività comune, divenendo protagonisti di tutte le fasi del loro compito (dalla pianificazione alla valutazione) mentre l’insegnante riveste il ruolo di facilitatore che aiuta nella comprensione delle richieste e organizza le attività. Il nostro studio ha voluto verificare se, un approccio metodologico non basato sulla ricerca estenuante della prestazione o sulla nota modularità di abilità o di discipline sportive, potesse coinvolgere i ragazzi durante le ore di educazione fisica. Partendo dall’indagine, si è quindi successivamente svolta un’analisi del grado di enjoyment registrato dagli studenti rispetto alle diverse proposte.

La scuola e lo sport sono due realtà che troppo spesso vengono considerate come unità a sé stanti, quando, invece, dovrebbero collaborare per migliorare gli studenti sia dal punto di vista intellettivo che fisico. Prendendo spunto da progetti già in atto in altri stati europei come la Finlandia e la Svizzera, dove sport e scuola viaggiano in parallelo da molti anni, nasce il progetto: “Smuovilascuola”. “Smuovilascuola” porta l’attività fisica nelle aule delle scuole primarie, facendo sì che diventi parte integrante delle lezioni curricolari, e non solo un accessorio per la palestra, così che si possano avere miglioramenti sia per l’area motoria che quella cognitiva dei bambini. Questi saranno più predisposti allo sport, più attenti in aula, coinvolti, e sapranno gestire meglio emozioni e paure. Lo scopo è educare gli studenti a 360°. Utilizzando dei test psicofisici validati si è riusciti a valutare come questo intervento sia riuscito ad aumentare l’autoefficacia degli studenti, il loro apprezzamento verso l’attività fisica e soprattutto la padronanza del loro corpo.

Doping poses a threat to sport worldwide. Studies have revealed that, in addition to elite athletes, amateur and recreational sportsmen and sportswomen are making increasing use of performance-enhancing drugs. Worryingly this trend has been documented among young people. Anti-doping efforts seeking to deter elite athletes from doping through detection of the use of prohibited substances are costly and have not been completely effective either at the top-level or the amateur/recreational level. A thoughtful education program, inspired by honesty and respect, might be more effective in shaping attitudes toward doping in young people and protecting their health. For these reasons, the Italian “Lotta al Doping” (Fight Against Doping) project sought to cause a cultural shift in young people by taking anti-doping seminars to high schools. In the 2017–2018 school year we reached more than 20,800 students from 157 high schools through 202 seminars. Before and after the seminars, we administrated anonymous, voluntarily completed surveys with a set of questions (items = 15), taken from the WADA-Play-True-Quiz. Upon completion of the 2-h seminar, the majority of the answers given by the students resulted correct (13 out of 15 items, p < 0.000001, McNemar) confirming the value of the initiative. This project stands out as promising in the doping prevention process at the youth and amateur levels.

PurposeThis study investigates the effect of a mentally demanding response inhibitory task on time trial performance in sub-elite under 23 cyclists.MethodsTen under 23 road cyclists completed two separate testing sessions during which they performed two different cognitive tasks before completing a 30-min time trial on the cycle ergometer. In the experimental condition, 30 min of a standard cognitive task (Stroop task) was used to elicit mental fatigue; in the control condition, a non-demanding activity was carried out. Subjective workload and mood were measured before and after the treatments, and motivation was recorded before the time-trial. During the time trial, power, cadence, heart rate, and rate of perceived exertion were assessed. Blood lactate concentrations and heart rate variability (using the root mean square of the successive differences) were measured before and after the time trial.ResultsThe Stroop task was rated more mentally (P < 0.001) and temporally (P < 0.001) demanding, effortful (P < 0.001), and frustrating (P = 0.001) than the control task; fatigue (P = 0.002) and vigor (P = 0.018) after the cognitive tasks were respectively higher and lower than in the control task. Mean power output (P = 0.007) and cadence (P = 0.043) were negatively affected by the Stroop task, while heart rate (P = 0.349), rating of perceived exertion (P = 0.710), blood lactate concentration (P = 0.850), and root mean square of the successive differences (P = 0.355) did not differ between the two conditions.ConclusionA mentally demanding activity reduced the subsequent physical performance in sub-elite under 23 cyclists. Thus, avoiding cognitive efforts before training and races could improve performance of high-level athletes.

Purpose: The aim of this study was to investigate objective and subjective sleep quality, daytime tiredness and sleepiness in response to a late-evening high intensity interval training (HIIT) session in neither-type soccer players that habitually trained late in the day. This is the first study that considered both athletes’ chronotype and habitual training time as crucial factors when assessing sleep quality in relation to an evening physical task.Methods: In this longitudinal, prospective, observational study, 14 Italian soccer players were recruited (mean age: 26.1 ± 4.5 years; height: 1.81 ± 0.06 m; weight: 78.9 ± 6.1 kg) and performed an extra-routine 4 × 4-min HIIT session at 09:00 p.m. Players used to train always between 09:00 and 11:00 p.m during the competitive season. All subjects wore an actigraph to evaluate their objective sleep parameters and a sleep diary was used to record subjective values of sleep quality, daytime tiredness, and daytime sleepiness. All data were analyzed as: the mean of the two nights before (PRE), the night after (POST 1), and the mean of the two nights after (POST 2) the extra-routine HIIT session. The subjects’ chronotype was assessed by the morningness-eveningness questionnaire (MEQ).Results: All players were classified as N-types (mean MEQ score: 49.4 ± 3.7). None of the actigraph parameters nor the subjective values of sleep quality, tiredness, and sleepiness showed significant changes in PRE, POST 1, and POST 2.Conclusion: The results of our study added more information regarding sleep quality outcomes in response to a late-evening HIIT session. Athletic trainers and medical staff should always control for chronotype and habitual training time when assessing variations to sleep quality in athletes.

La scrittura a mano è un’abilità che richiede una serie di competenze motorie finalizzate a collegare e collocare nello spazio grafico le lettere secondo specifiche convenzioni. Richiedendo un elevato livello di coordinazione e una regolazione della contrazione muscolare ad alta precisione è conseguente la riflessione che una povera coordinazione generale e un scarso livello di controllo motorio ne influenzi la sua corretta espressione. Da qui si identifica la disgrafia come un disturbo specifico di apprendimento legato ad un deficit di natura esclusivamente motoria. Il nostro studio si pone lo scopo di affrontare il problema grafico come una dinamica spaziotemporale all’interno di uno spazio geometrico ben definito attraverso l’esperienza di tutto il corpo. Sottoponendo ad alunni disgrafici un percorso motorio di 12 lezioni incentrato sulla presa di coscienza del proprio schema corporeo/motorio, sul miglioramento della coordinazione oculo-manuale e spazio-temporale si è potuto verificare la possibilità di ottenere un transfer tra lo spazio vissuto e lo spazio del foglio. L’evidente miglioramento della produzione grafica dopo un percorso motorio realizzato ‘total-body’ in palestra sono indicativi di una possibilita’ da perseguire precocemente.

This study determined the reliability of post-exercise heart rate recovery (HRR) and vagal-related HR variability (HRV) after repeated-sprints (RSs), and contrasted it with the smallest worthwhile change (SWC) of these indices. Fourteen healthy male participants performed on four occasions, separated by 7 days, five 30-m sprints interspersed by 25-s of recovery. Post-exercise HR during 10 min of seated rest was measured. HRR during the first 60-s of recovery was computed (HRR60s). HRV indices were calculated in time and frequency domains during the last 5-min of the recovery. Absolute and relative reliability were assessed by typical error of measurement expressed as coefficient of variation (CV) and intraclass correlation coefficients (ICCs), respectively. Sensitivity was assessed comparing SWC to the typical error of measurement. CV ranged from 3.6% to 13.5% and from 6.3% to 109.2% for the HRR and HRV indices, respectively. ICCs were from 0.78 to 0.96 and from 0.76 to 0.92, respectively. HRR and HRV indices showed large discrepancies reliability. HRR60s and the square root of the mean sum of the squared differences between R-R intervals presented the highest levels of both absolute and relative reliability. However, SWC was lower than the typical error of measurement, indicating insufficient sensitivity to confidently detect small, but meaningful, changes in HRR and HRV indices.

The 6-minute walking test (6MWT) is a simple assessment tool to evaluate exercise capacity. The result of the test is the distance that a subject can walk at a constant and normal pace within 6 minutes (6MWD) and reflects the aerobic/fitness performance related to walking function. Use of 6MWT has been relevant to assess exercise tolerance either in healthy children or in patients with, heart, lung and metabolic diseases. Our aim was to find 6MWT reference values in healthy Italian children. The 6MWT was performed in 5614 children aged 6-11 years recruited from primary Italian schools. Age related reference percentiles of the covered distance were gender-modeled. A linear and quadratic regression model was used to predict 6MWT performance. Males walked longer distances than females, respectively 598.8±83.9 m vs 592.1±77.6 m (p = 0.0016). According to the regression analysis, 6MWD was positively related to age, gender and height, while it was negatively related to body weight [(6MWD = -160.16 + 93.35× age (years) -4.05× age2 (years) +7.34× gender (m) +2.12× weight (kg) -2.50× height (cm)]. Reference values were established for the 6MWT in healthy children. The age related 6MWD percentiles provided a useful tool in the assessment of capacity in 6-11 year children, in fact they may be helpful to evaluate the effect of a given treatment or rehabilitation program and represent a feasible measure as to prevention within the primary school context. It was found a substantial difference from other countries for 6mwd values. In our study, factors such as age, weight and height were relevant for the prediction of 6MWD, similarly to other studies. Therefore, these variables should be taken into account in context of exercise performance.

Purpose: The aim of this study was to compare the effects of continuous moderate-intensity and discontinuous high-intensity training on running performance in master runners.Methods: Thirty-four male master runners (47.2 ± 7.4 years) were assigned to three different groups: continuous moderate-intensity training (CMIT), discontinuous high-intensity training (DHIT), and control group (CON). CMIT and DHIT performed 8-week of supervised training (3 session·wk−1; ~35 km·wk−1) while CON maintained their normal training habits (3–4 session·wk−1; ~50 km·wk−1). Peak oxygen consumption (V˙O2peak) and peak running speed (vpeak) during incremental treadmill exercise, gas exchange threshold (GET), speed at GET, energy cost of running (Cr), and 5-km performance were evaluated before and after training.Results: Following the training period, both CMIT and DHIT significantly reduced Cr (−4.4 and −4.9%, respectively, P < 0.05), increased speed at GET (3.4 and 5.7%, P < 0.05) and improved 5-km time-trial performance (3.1 and 2.2%, P < 0.05) whereas no differences were found for V˙O2peak and GET (as %V˙O2peak). After training, vpeak improved only for DHIT (6%, P < 0.05). No differences were found in any variable for CON.Conclusions: This study indicates that both CMIT and DHIT may positively affect running performance in middle-aged master runners. This improvement was achieved despite a significant reduction of the amount of weekly training volume.

[This corrects the article DOI: 10.1371/journal.pone.0205792.].

Purpose: To provide insights into the energy requirements as well as the physiological adaptations of an experienced 50-year-old ultra-marathon male athlete during the world's most challenging mountain ultra-marathon (MUM).Methods: The international race supporting the study was the Tor des Geants®, characterized by 330 km with +24,000 m D+ to be covered within 150 h. Before the MUM, we assessed the peak oxygen uptake (V˙O2peak) by means of an incremental graded running test. During the MUM we monitored six ascents (once per race day) with a portable gas analyzer, a GPS and a finger pulse oximeter. We then calculated the net metabolic cost per unit of distance (C), the vertical metabolic cost (Cvert) and the mechanical efficiency of locomotion (Effmech) throughout the six uphills monitored. We further monitored the distance covered, speed, altimetry and D+ from the GPS data as well as the pulse oxygen saturation with the finger pulse oximeter.Results: Subject's V˙O2peak was 48.1 mL·kg−1·min−1. Throughout the six uphills investigated the mean exercise intensity was 57.3 ± 6.0% V˙O2peak and 68.0 ± 8.7% HRpeak. C, Cvert and Effmech were 11.4 ± 1.9 J·kg−1·m−1, 57.9 ± 15.2 J·kg−1·mvert−1, and 17.7 ± 4.8%, respectively. The exercise intensity, as well as C, Cvert, and Effmech did not consistently increase during the MUM.Conclusions: For the first time, we described the feasibility of assessing the energy requirements as well as the physiological adaptations of a MUM in ecologically valid environment settings. The present case study shows that, despite the distance performed during the MUM, our participant did not experience a metabolic fatigue state. This is likely due to improvements in locomotor efficiency as the race progressed.

The aim of this study was to investigate the influence of chronotype on mood state and ratings of perceived exertion (RPE) before and in response to acute high intensity interval exercise (HIIE) performed at different times of the day. Based on the morningness–eveningness questionnaire, 12 morning-types (M-types; N = 12; age 21 ± 2 years; height 179 ± 5 cm; body mass 74 ± 12 kg) and 11 evening-types (E-types; N = 11; age 21 ± 2 years; height 181 ± 11 cm; body mass 76 ± 11 kg) were enrolled in a randomized crossover study. All subjects underwent measurements of Profile of Mood States (POMS), before (PRE), after 12 (POST12) and 24 h (POST24) the completion of both morning (08.00 am) and evening (08.00 p.m.) training. Additionally, Global Mood Disturbance and Energy Index (EI) were calculated. RPE was obtained PRE and 30 min POST HIIE. Two-way ANOVA with Tukey’s multiple comparisons test of POMS parameters during morning training showed significant differences in fatigue, vigor and EI at PRE and POST24 between M-types and E-types. In addition, significant chronotype differences were found only in POST12 after the evening HIIE for fatigue, vigor and EI. For what concerns Borg perceived exertion, comparing morning versus evening values in PRE condition, a higher RPE was observed in relation to evening training for M-types (P = 0.0107) while E-types showed higher RPE values in the morning (P = 0.008). Finally, intragroup differences showed that E-types had a higher RPE respect to M-types before (P = 0.002) and after 30 min (P = 0.042) the morning session of HIIE. No significant changes during the evening training session were found. In conclusion, chronotype seems to significantly influence fatigue values, perceived exertions and vigor in relation to HIIE performed at different times of the day. Specifically, E-types will meet more of a burden when undertaking a physical task early in the day. Practical results suggest that performing a HIIE at those times of day that do not correspond to subjects’ circadian preference can lead to increased mood disturbances and perceived exertion. Therefore, an athlete’s chronotype should be taken into account when scheduling HIIE.Trial registration:ACTRN12617000432314, registered 24 March 2017, “retrospectively registered”.Web address of trial:https://www.anzctr.org.au/Trial/Registration/TrialReview.aspx?id=371862&showOriginal=true&isReview=true

Background: It has been shown that the fastest open-water swimmers (OW-swimmers) increase significantly the speed in the last split of the open-water events. The aim of the present work was to determine if the fastest OW-swimmers have a higher speed in the middle- and long-distance pool swimming events, and to develop a multivariate model that can predict the medalist group in the 10-km competition. Methods: A total of 484 athletes (252-males and 232-females) were included in the analysis. Swimmers were divided into four groups based on their finishing position in the competition. For each swimmer, the absolute best performance (PB) of 200, 400, 800 and 1500-meter in long course, the seasonal best performance (SPB) obtained before the open-water events and critical velocity (CV) were analyzed. Multivariate analysis of variance (MANOVA) was used to detect significant differences between groups and discriminant analysis was used to predict a grouping variable. Results: All the variables analyzed were significantly different between groups (p < 0.001). The first discriminant function correctly classified 50% of the overall female and male swimmers. Conclusion: Fastest OW-swimmers have a higher speed in middle- and long-distance pool swimming events. Further studies should include different anthropometric and physiological variables to increase the accuracy of classification.

[This corrects the article DOI: 10.1371/journal.pone.0170744.].

The idea that early sport success can be detrimental for long-term sport performance is still under debate. Therefore, the aims of this study were to examine the career trajectories of Italian high and long jumpers to provide a better understanding of performance development in jumping events.The official long-jump and high-jump rankings of the Italian Track and Field Federation were collected from the age of 12 to career termination, for both genders from the year 1994 to 2014. Top-level athletes were identified as those with a percentile of their personal best performance between 97 and 100.The age of entering competitions of top-level athletes was not different than the rest of the athletic population, whereas top-level athletes performed their personal best later than the rest of the athletes. Top-level athletes showed an overall higher rate of improvement in performance from the age of 13 to the age of 18 years when compared to all other individuals. Only 10-25% of the top-level adult athletes were top-level at the age of 16. Around 60% of the top-level young at the age of 16 did not maintain the same level of performance in adulthood. Female high-jump represented an exception from this trend since in this group most top-level young become top-level adult athletes.These findings suggest that performance before the age of 16 is not a good predictor of adult performance in long and high jump. The annual rate of improvements from 13 to 18 years should be included as a predictor of success rather than performance per se. Coaches should be careful about predicting future success based on performances obtained during youth in jumping events.

Purpose: To examine the effects of the world’s most challenging mountain ultramarathon (MUM, 330 km, cumulative elevation gain of +24000 m) on the energy cost and kinematics of different uphill gaits. Methods: Before (PRE) and immediately after (POST) the competition, 19 male athletes performed three submaximal 5-min treadmill exercise trials in a randomized order: walking at 5 km·h-1, +20%; running at 6 km·h-1, +15%; and running at 8 km·h-1, +10%. During the three trials, energy cost was assessed using an indirect calorimetry system and spatiotemporal gait parameters were acquired with a floor-level high-density photoelectric cells system. Results: The average time of the study participants to complete the MUM was 129 h 43 min 48 s (range: 107 h 29 min 24 s to 144 h 21 min 0 s). Energy costs in walking (-11.5 ± 5.5%, P < 0.001), as well as in the first (-7.2 ± 3.1%, P = 0.01) and second (-7.0 ± 3.9%, P = 0.02) running condition decreased between PRE and POST, with a reduction both in the heart rate (-11.3%, -10.0%, and -9.3%, respectively) and oxygen uptake only for the walking condition (-6.5%). No consistent and significant changes in the kinematics variables were detected (P values from 0.10 to 0.96). Conclusion: Though fatigued after completing the MUM, the subjects were still able to maintain their uphill locomotion patterns noted at PRE. The decrease (improvement) in the energy costs was likely due to the prolonged and repetitive walking/running, reflecting a generic improvement in the mechanical efficiency of locomotion after ~130 h of uphill locomotion rather than constraints imposed by the activity on the musculoskeletal structure and function.

Cardio-respiratory fitness (CRF) is a widespread essential indicator in Sports Science as well as in Sports Medicine. This study aimed to develop and validate a prediction model for CRF based on a 45 second self-test, which can be conducted anywhere. Criterion validity, test re-test study was set up to accomplish our objectives. Data from 81 healthy volunteers (age: 29 ± 8 years, BMI: 24.0 ± 2.9), 18 of whom females, were used to validate this test against gold standard. Nineteen volunteers repeated this test twice in order to evaluate its repeatability. CRF estimation models were developed using heart rate (HR) features extracted from the resting, exercise, and the recovery phase. The most predictive HR feature was the intercept of the linear equation fitting the HR values during the recovery phase normalized for the height2 (r2 = 0.30). The Ruffier-Dickson Index (RDI), which was originally developed for this squat test, showed a negative significant correlation with CRF (r = -0.40), but explained only 15% of the variability in CRF. A multivariate model based on RDI and sex, age and height increased the explained variability up to 53% with a cross validation (CV) error of 0.532 L ∙ min-1 and substantial repeatability (ICC = 0.91). The best predictive multivariate model made use of the linear intercept of HR at the beginning of the recovery normalized for height2 and age2; this had an adjusted r2 = 0. 59, a CV error of 0.495 L·min-1 and substantial repeatability (ICC = 0.93). It also had a higher agreement in classifying CRF levels (κ = 0.42) than RDI-based model (κ = 0.29). In conclusion, this simple 45 s self-test can be used to estimate and classify CRF in healthy individuals with moderate accuracy and large repeatability when HR recovery features are included.

Beneficial systemic effects of regular physical exercise have been demonstrated to reduce risks of a number of age-related disorders. Antioxidant capacity adaptations are amongst these fundamental changes in response to exercise training. However, it has been claimed that acute physical exercise performed at high intensity (>60% of maximal oxygen uptake) may result in oxidative stress, due to reactive oxygen species being generated excessively by enhanced oxygen consumption. The aim of this study was to evaluate the effect of high-intensity discontinuous training (HIDT), characterized by repeated variations of intensity and changes of redox potential, on oxidative damage. Twenty long-distance masters runners (age 47.8 ± 7.8 yr) on the basis of the individual values of gas exchange threshold were assigned to a different 8-weeks training program: continuous moderate-intensity training (MOD, n = 10) or HIDT (n = 10). In both groups before (PRE) and after (POST) training we examined the following oxidative damage markers: thiobarbituric acid reactive substances (TBARS) as marker of lipid peroxidation; protein carbonyls (PC) as marker of protein oxidation; 8-hydroxy-2-deoxy-guanosine (8-OH-dG) as a biomarker of DNA base modifications; and total antioxidant capacity (TAC) as indicator of the overall antioxidant system. Training induced a significant (p