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Your search keyword '"Aparicio, I.M."' showing total 20 results
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20 results on '"Aparicio, I.M."'

14. Apoptosis related protein profile in cooled stallion semen

Author

Gallardo-Bolaños, J.M., primary, Morillo-Rodriguez, A., additional, Balao-da Silva, C., additional, Ortega-Ferrusola, C., additional, Plaza-Dávila, M., additional, Miro-Morán, A., additional, Tapia, J.A., additional, Aparicio, I.M., additional, and Peña, F.J., additional

Published
2012
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20. Boar sperm velocity and motility patterns under capacitating and non-capacitating incubation conditions

Author

García Herreros, M., Aparicio, I.M., Núñez, I., García-Marín, L.J., Gil, M.C., and Peña Vega, F.J.

Subjects
*BOARS, *SPERMATOZOA, *CELL motility, *GERM cells
Abstract

This study compares the velocity and motility of boar sperm under capacitating and non-capacitating incubation conditions. Aliquots of pooled, washed boar sperm were incubated in either Tyrode’s complete medium (TCM; a capacitating medium), Ca2+-free TCM (TCM-Ca2+), or Ca2+ and NaHCO3-free TCM (Tyrode’s basal medium [TBM]; a non-capacitating medium). Motility patterns were determined every hour over a 3 h period of incubation at 38 °C. Capacitation status was assessed by the chlortetracycline assay after 1 and 3 h of incubation. Experiments were repeated five times.Compared to the TBM control, a significant increase was seen in the percentage of capacitated sperm after 1 h of incubation in TCM: the kinematics of these sperm cells were favorably modified. However, the motility patterns of sperm cells incubated in TCM and TCM-Ca2+ were very similar. Under capacitating conditions (TCM), the coefficients of linearity (LIN) and straightness (STR) significantly increased over time (LIN values were significantly different after 3 h of incubation, while STR values were significantly different after only 2 h). Significant correlations were seen between LIN and the percentage of cells showing the B pattern (r = 0.334, P < 0.05) and the number of acrosome reacted spermatozoa (r = 0.301, P < 0.05). This suggests that capacitated boar spermatozoa may have a species-specific motility pattern. [Copyright &y& Elsevier]

Published
2005
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