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Your search keyword '"Aparin, Ilya O."' showing total 29 results
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29 results on '"Aparin, Ilya O."'

2. Compounds based on 5-(perylen-3-ylethynyl)uracil scaffold: High activity against tick-borne encephalitis virus and non-specific activity against enterovirus A

Author

Chistov, Alexey A., Orlov, Alexey A., Streshnev, Philipp P., Slesarchuk, Nikita A., Aparin, Ilya O., Rathi, Brijesh, Brylev, Vladimir A., Kutyakov, Sergey V., Mikhura, Irina V., Ustinov, Alexey V., Westman, Gunnar, Palyulin, Vladimir A., Jain, Nidhi, Osolodkin, Dmitry I., Kozlovskaya, Liubov I., and Korshun, Vladimir A.

Published
2019
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17. Toehold-Mediated Selective Assembly of Compact Discrete DNA Nanostructures

Author

Brylev, Vladimir A., primary, Ustinov, Alexey V., additional, Tsvetkov, Vladimir B., additional, Barinov, Nikolay A., additional, Aparin, Ilya O., additional, Sapozhnikova, Ksenia A., additional, Berlina, Yana Y., additional, Kokin, Egor A., additional, Klinov, Dmitry V., additional, Zatsepin, Timofei S., additional, and Korshun, Vladimir A., additional

Published
2020
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23. Automated Solid-Phase Click Synthesis of Oligonucleotide Conjugates: From Small Molecules to Diverse N-Acetylgalactosamine Clusters

Author

Farzan, Valentina M., primary, Ulashchik, Egor A., additional, Martynenko-Makaev, Yury V., additional, Kvach, Maksim V., additional, Aparin, Ilya O., additional, Brylev, Vladimir A., additional, Prikazchikova, Tatiana A., additional, Maklakova, Svetlana Yu., additional, Majouga, Alexander G., additional, Ustinov, Alexey V., additional, Shipulin, German A., additional, Shmanai, Vadim V., additional, Korshun, Vladimir A., additional, and Zatsepin, Timofei S., additional

Published
2017
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25. Fine Tuning of Pyrene Excimer Fluorescence in Molecular Beacons by Alteration of the Monomer Structure

Author

Aparin, Ilya O., Proskurin, Gleb V., Golovin, Andrey V., Ustinov, Alexey V., Formanovsky, Andrey A., Zatsepin, Timofei S., and Korshun, Vladimir A.

Abstract

Oligonucleotide probes labeled with pyrene pairs that form excimers have a number of applications in hybridization analysis of nucleic acids. A long excited state lifetime, large Stokes shift, and chemical stability make pyrene excimer an attractive fluorescent label. Here we report synthesis of chiral phosphoramidite building blocks based on (R)-4-amino-2,2-dimethylbutane-1,3-diol, easily available from an inexpensive d-(−)-pantolactone. 1-Pyreneacetamide, 1-pyrenecarboxamide, and DABCYL derivatives have been used in preparation of molecular beacon (MB) probes labeled with one or two pyrenes/quenchers. We observed significant difference in the excimer emission maxima (475–510 nm; Stokes shifts 125–160 nm or 7520–8960 cm–1) and excimer/monomer ratio (from 0.5 to 5.9) in fluorescence spectra depending on the structure and position of monomers in the pyrene pair. The pyrene excimer formed by two rigid 1-pyrenecarboxamide residues showed the brightest emission. This is consistent with molecular dynamics data on excimer stability. Increase of the excimer fluorescence for MBs after hybridization with DNA was up to 24-fold.

Published
2024
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27. 1-Phenylethynylpyrene (PEPy) as a novel blue-emitting dye for qPCR assay

Author

Aparin, Ilya O., primary, Farzan, Valentina M., additional, Veselova, Olga A., additional, Chistov, Alexey A., additional, Podkolzin, Alexander T., additional, Ustinov, Alexey V., additional, Shipulin, German A., additional, Formanovsky, Andrey A., additional, Korshun, Vladimir A., additional, and Zatsepin, Timofei S., additional

Published
2016
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28. Blue-emitting fluorescent azides for DNA labelling

Author

Aparin, Ilya O., primary, Chistov, Alexey A., additional, Prokhorenko, Igor A., additional, Stepanova, Irina A., additional, Veselova, Olga A., additional, Ustinov, Alexey V., additional, Formanovsky, Andrey A., additional, Zatsepin, Timofey S., additional, and Korshun, Vladimir A., additional

Published
2014
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29. Counterion-insulated near-infrared dyes in biodegradable polymer nanoparticles for in vivo imaging.

Author

Sobska J, Andreiuk B, Aparin IO, Reisch A, Krezel W, and Klymchenko AS

Abstract

Polymeric nanoparticles (NPs) are highly attractive for biomedical applications due to their potential biodegradability and capacity to encapsulate different loads, notably drugs and contrast agents. For in vivo optical bioimaging, NPs should operate in the near-infrared region (NIR) and exhibit stealth properties. In the present work, we applied the approach of ionic dye insulation with bulky hydrophobic counterions for encapsulation of near-infrared cyanine dyes (Cy5.5 and Cy7 bearing two octadecyl chains) into biodegradable polymer (PLGA) NPs. We found that at high dye loading (20-50 mM with respect to the polymer), the bulkiest fluorinated tetraphenylborate counterion minimized best the aggregation-caused quenching and improved fluorescence quantum yields of both NIR dyes, especially of Cy5.5. In addition, bulky counterions also enabled formation of small 40 nm polymeric NPs in contrast to smaller counterions. To provide them stealth properties, we prepared 40 nm dye-loaded PEGylated NPs through nanoprecipitation of synthetic PLGA-PEG block copolymer with the dye/counterion salt. The obtained NIR NPs loaded with Cy5.5 dye salt allowed in vivo imaging of wild-type mice with a good contrast after IV injection. Compared to the bare PLGA NPs, PLGA-PEG NPs exhibited significantly slower accumulation in the liver. Biodistribution studies confirmed the preferential accumulation in the liver, although PLGA and PLGA-PEG NPs could also be distributed in other organs, with the following tendency: liver > spleen > lungs > kidney > heart > testis > brain. Overall, the present work validated the counterion approach for encapsulation of NIR cyanine dyes into biodegradable polymer NPs bearing covalently attached PEG shell. Thus, we propose a simple and robust methodology for preparation of NIR fluorescent biodegradable polymer NPs, which could further improve the existing optical imaging for biomedical applications., Competing Interests: There are no conflicts to declare., (This journal is © The Royal Society of Chemistry.)

Published
2021
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