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48 results on '"Apostolova, Margarita D."'

1. A template wizard for the cocreation of machine-readable data-reporting to harmonize the evaluation of (nano)materials

Author

European Commission, Slovenian Research Agency, Norwegian Research Council, Jeliazkova, Nina [0000-0002-4322-6179], Longhin, Eleonora [0000-0002-3939-7135], El Yamani, Naouale [0000-0001-5456-8230], Rundén-Pran, Elise [0000-0002-5541-4513], Moschini, Elisa [0000-0001-9825-6263], Serchi, Tommaso [0000-0003-2386-3514], Vrček, Ivana Vinković [0000-0003-1382-5581], Burgum, Michael J. [0000-0002-3123-8914], Doak, Shareen H. [0000-0002-6753-1987], Cimpan, Mihaela Roxana [0000-0003-2029-3173], Battistelli, Chiara [0000-0003-2386-0727], Bossa, Cecilia [0000-0003-2084-2902], Tsekovska, Rositsa [0000-0001-5431-5743], Drobne, Damjana [0000-0002-5970-7460], Novak, Sara [0000-0003-0026-456X], Repar, Neža [0000-0003-4310-0362], Ammar, Ammar [0000-0002-8399-8990], Nymark, Penny [0000-0002-3435-7775], Di Battista, Veronica [0000-0002-6491-9185], Sosnowska, Anita [0000-0003-3288-4309], Puzyn, Tomasz [0000-0003-0449-8339], Kochev, Nikolay [0000-0001-6547-3675], Iliev, Luchesar [0000-0001-7000-8890], Jeliazkov, Vedrin [0000-0002-1520-5995], Reilly, Katie [0000-0002-6054-0645], Lynch, Iseult [0000-0003-4250-4584], Bakker, Martine [0000-0001-8379-7016], Delpivo, Camila [0000-0002-4413-7602], Sánchez-Jiménez, A. [0000-0002-2625-9155], Fonseca, Ana Sofia [0000-0002-1424-6365], Manier, Nicolas [0000-0003-3873-6657], Fernández-Cruz, M. L. [0000-0001-5988-1939], Rashid, Shahzad [0009-0002-3525-6607], Willighagen, Egon L. [0000-0001-7542-0286], Apostolova, Margarita D. [0000-0001-7188-506X], Dusinska, Maria [0000-0003-1358-1652], Jeliazkova, Nina, Longhin, Eleonora, El Yamani, Naouale, Rundén-Pran, Elise, Moschini, Elisa, Serchi, Tommaso, Vrček, Ivana Vinković, Burgum, Michael J., Doak, Shareen H., Cimpan, Mihaela Roxana, Rios-Mondragon, Ivan, Cimpan, Emil, Battistelli, Chiara, Bossa, Cecilia, Tsekovska, Rositsa, Drobne, Damjana, Novak, Sara, Repar, Neža, Ammar, Ammar, Nymark, Penny, Di Battista, Veronica, Sosnowska, Anita, Puzyn, Tomasz, Kochev, Nikolay, Iliev, Luchesar, Jeliazkov, Vedrin, Reilly, Katie, Lynch, Iseult, Bakker, Martine, Delpivo, Camila, Sánchez-Jiménez, A., Fonseca, Ana Sofia, Manier, Nicolas, Fernández-Cruz, M. L., Rashid, Shahzad, Willighagen, Egon L., Apostolova, Margarita D., Dusinska, Maria, European Commission, Slovenian Research Agency, Norwegian Research Council, Jeliazkova, Nina [0000-0002-4322-6179], Longhin, Eleonora [0000-0002-3939-7135], El Yamani, Naouale [0000-0001-5456-8230], Rundén-Pran, Elise [0000-0002-5541-4513], Moschini, Elisa [0000-0001-9825-6263], Serchi, Tommaso [0000-0003-2386-3514], Vrček, Ivana Vinković [0000-0003-1382-5581], Burgum, Michael J. [0000-0002-3123-8914], Doak, Shareen H. [0000-0002-6753-1987], Cimpan, Mihaela Roxana [0000-0003-2029-3173], Battistelli, Chiara [0000-0003-2386-0727], Bossa, Cecilia [0000-0003-2084-2902], Tsekovska, Rositsa [0000-0001-5431-5743], Drobne, Damjana [0000-0002-5970-7460], Novak, Sara [0000-0003-0026-456X], Repar, Neža [0000-0003-4310-0362], Ammar, Ammar [0000-0002-8399-8990], Nymark, Penny [0000-0002-3435-7775], Di Battista, Veronica [0000-0002-6491-9185], Sosnowska, Anita [0000-0003-3288-4309], Puzyn, Tomasz [0000-0003-0449-8339], Kochev, Nikolay [0000-0001-6547-3675], Iliev, Luchesar [0000-0001-7000-8890], Jeliazkov, Vedrin [0000-0002-1520-5995], Reilly, Katie [0000-0002-6054-0645], Lynch, Iseult [0000-0003-4250-4584], Bakker, Martine [0000-0001-8379-7016], Delpivo, Camila [0000-0002-4413-7602], Sánchez-Jiménez, A. [0000-0002-2625-9155], Fonseca, Ana Sofia [0000-0002-1424-6365], Manier, Nicolas [0000-0003-3873-6657], Fernández-Cruz, M. L. [0000-0001-5988-1939], Rashid, Shahzad [0009-0002-3525-6607], Willighagen, Egon L. [0000-0001-7542-0286], Apostolova, Margarita D. [0000-0001-7188-506X], Dusinska, Maria [0000-0003-1358-1652], Jeliazkova, Nina, Longhin, Eleonora, El Yamani, Naouale, Rundén-Pran, Elise, Moschini, Elisa, Serchi, Tommaso, Vrček, Ivana Vinković, Burgum, Michael J., Doak, Shareen H., Cimpan, Mihaela Roxana, Rios-Mondragon, Ivan, Cimpan, Emil, Battistelli, Chiara, Bossa, Cecilia, Tsekovska, Rositsa, Drobne, Damjana, Novak, Sara, Repar, Neža, Ammar, Ammar, Nymark, Penny, Di Battista, Veronica, Sosnowska, Anita, Puzyn, Tomasz, Kochev, Nikolay, Iliev, Luchesar, Jeliazkov, Vedrin, Reilly, Katie, Lynch, Iseult, Bakker, Martine, Delpivo, Camila, Sánchez-Jiménez, A., Fonseca, Ana Sofia, Manier, Nicolas, Fernández-Cruz, M. L., Rashid, Shahzad, Willighagen, Egon L., Apostolova, Margarita D., and Dusinska, Maria

Abstract

Making research data findable, accessible, interoperable and reusable (FAIR) is typically hampered by a lack of skills in technical aspects of data management by data generators and a lack of resources. We developed a Template Wizard for researchers to easily create templates suitable for consistently capturing data and metadata from their experiments. The templates are easy to use and enable the compilation of machine-readable metadata to accompany data generation and align them to existing community standards and databases, such as eNanoMapper, streamlining the adoption of the FAIR principles. These templates are citable objects and are available as online tools. The Template Wizard is designed to be user friendly and facilitates using and reusing existing templates for new projects or project extensions. The wizard is accompanied by an online template validator, which allows self-evaluation of the template (to ensure mapping to the data schema and machine readability of the captured data) and transformation by an open-source parser into machine-readable formats, compliant with the FAIR principles. The templates are based on extensive collective experience in nanosafety data collection and include over 60 harmonized data entry templates for physicochemical characterization and hazard assessment (cell viability, genotoxicity, environmental organism dose-response tests, omics), as well as exposure and release studies. The templates are generalizable across fields and have already been extended and adapted for microplastics and advanced materials research. The harmonized templates improve the reliability of interlaboratory comparisons, data reuse and meta-analyses and can facilitate the safety evaluation and regulation process for (nano) materials.

Published
2024

2. One-Step Magneton Sputtering of Crystalline Cu-Doped TiO 2 Coatings: Characterization and Antibacterial Activity.

Author

Nikolova, Maria P., Yousefi, Sadegh, Handzhiyski, Yordan, and Apostolova, Margarita D.

Subjects
ESCHERICHIA coli, CONTACT angle, ATMOSPHERIC oxygen, DC sputtering, SUBSTRATES (Materials science)
Abstract

Early biofilm formation could be inhibited by applying a thin biocompatible copper coating to reduce periprosthetic infections. In this study, we deposited crystalline Cu-doped TiO2 films using one-step DC magnetron sputtering in an oxygen atmosphere on a biased Ti6Al4V alloy without external heating. The bias voltage varied from −25 V to −100 V, and the resultant substrate temperature was measured. The deposited coatings were characterized using X-ray diffraction (XRD), scanning electron microscopy (SEM), microhardness, scratch and hydrophilicity tests, potentiodynamic polarization measurements, and antibacterial assays against S. aureus and E. coli. The findings demonstrated that when a higher negative bias is applied, the substrate temperature drops, and the anatase to rutile transformation is initiated without indicating obvious Cu-containing phases. The SEM images of the films showed spherical agglomerates with homogeneously distributed Cu with decreasing Cu content as the bias value increased. Higher bias results in the grain refinement of the thinning coatings with more lattice microstrain and more defects, together with an increase in water contact angles and hardness values. Samples biased at −75 V exhibited the highest adhesive strength between coatings and substrate, whereas the specimen biased at −50 V demonstrated higher corrosion resistance. Cu-containing TiO2 coatings with pure anatase phase composition and Cu concentrations of 2.62 wt.% demonstrated excellent bactericidal activity against both S. aureus and E. coli. The layers containing 2.34 wt.% Cu exhibited very good antibacterial properties against S. aureus, only. According to these findings, the produced copper-doped TiO2 coatings have high bactericidal qualities in vitro and may be used to prepare orthopaedic and dental implants in the future. [ABSTRACT FROM AUTHOR]

Published
2024
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6. Towards FAIR nanosafety data

Author

Jeliazkova, Nina, Apostolova, Margarita D., Andreoli, Cristina, Barone, Flavia, Barrick, Andrew, Battistelli, Chiara, Bossa, Cecilia, Botea-Petcu, Alina, Châtel, Amélie, De Angelis, Isabella, Dusinska, Maria, El Yamani, Naouale, Gheorghe, Daniela, Giusti, Anna, Gómez-Fernández, Paloma, Grafström, Roland, Gromelski, Maciej, Jacobsen, Nicklas Raun, Jeliazkov, Vedrin, Jensen, Keld Alstrup, Kochev, Nikolay, Kohonen, Pekka, Manier, Nicolas, Mariussen, Espen, Mech, Agnieszka, Navas, José María, Paskaleva, Vesselina, Precupas, Aurica, Puzyn, Tomasz, Rasmussen, Kirsten, Ritchie, Peter, Llopis, Isabel Rodríguez, Rundén-Pran, Elise, Sandu, Romica, Shandilya, Neeraj, Tanasescu, Speranta, Haase, Andrea, and Nymark, Penny

Published
2021
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7. Effect of Co-Sputtered Copper and Titanium Oxide Coatings on Bacterial Resistance and Cytocompatibility of Osteoblast Cells.

Author

Nikolova, Maria P., Tzvetkov, Iliyan, Dimitrova, Tanya V., Ivanova, Veronika L., Handzhiyski, Yordan, Andreeva, Andreana, Valkov, Stefan, Ormanova, Maria, and Apostolova, Margarita D.

Subjects
TITANIUM oxides, COPPER-titanium alloys, OXIDE coating, DRUG resistance in bacteria, CONTACT angle, RUTILE
Abstract

One of the primary risk factors for implant failure is thought to be implant-related infections during the early healing phase. Developing coatings with cell stimulatory behaviour and bacterial adhesion control is still difficult for bone implants. This study proposes an approach for one-step deposition of biocompatible and antimicrobial Cu-doped TiO2 coatings via glow-discharge sputtering of a mosaic target. During the deposition, the bias of the Ti6Al4V substrates was changed. Structure examination, phase analysis, and surface morphology were carried out using X-ray diffraction (XRD) analysis, scanning electron microscopy (SEM), atomic force microscopy (AFM), and X-ray photoelectron spectroscopy (XPS). The hardness values and hydrophilic and corrosion performance were also evaluated together with cytocompatible and antibacterial examinations against E. coli and S. aureus. The results show great chemical and phase control of the bias identifying rutile, anatase, CuO, or ternary oxide phases. It was found that by increasing the substrate bias from 0 to −50 V the Cu content increased from 15.3 up to 20.7 at% while at a high bias of −100 V, the copper content reduced to 3 at%. Simultaneously, apart from the Cu2+ state, Cu1+ is also found in the biased samples. Compared with the bare alloy, the hardness, the water contact angle and corrosion resistance of the biased coatings increased. According to an assessment of in vitro cytocompatibility, all coatings were found to be nontoxic to MG-63 osteoblast cells over the time studied. Copper release and cell-surface interactions generated an antibacterial effect against E. coli and S. aureus strains. The −50 V biased coating combined the most successful results in inhibiting bacterial growth and eliciting the proper responses from osteoblastic cells because of its phase composition, electrochemical stability, hydrophilicity, improved substrate adhesion, and surface roughness. Using this novel surface modification approach, we achieved multifunctionality through controlled copper content and oxide phase composition in the sputtered films. [ABSTRACT FROM AUTHOR]

Published
2024
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8. Safe(r) by design implementation in the nanotechnology industry

Author

Sánchez Jiménez, Araceli, Puelles, Raquel, Pérez-Fernández, Marta, Gómez-Fernández, Paloma, Barruetabeña, Leire, Jacobsen, Nicklas Raun, Suarez-Merino, Blanca, Micheletti, Christian, Manier, Nicolas, Trouiller, Bénédicte, Navas, Jose Maria, Kalman, Judit, Salieri, Beatrice, Hischier, Roland, Handzhiyski, Yordan, Apostolova, Margarita D., Hadrup, Niels, Bouillard, Jacques, Oudart, Yohan, Merino, Cesar, Garcia, Erika, Liguori, Biase, Sabella, Stefania, Rose, Jerome, Masion, Armand, Galea, Karen S., Kelly, Sean, Štěpánková, Sandra, Mouneyrac, Catherine, Barrick, Andrew, Châtel, Amélie, Dusinska, María, Rundén-Pran, Elise, Mariussen, Espen, Bressot, Christophe, Aguerre-Chariol, Olivier, Shandilya, Neeraj, Goede, Henk, Gomez-Cordon, Julio, Simar, Sophie, Nesslany, Fabrice, Jensen, Keld Alstrup, van Tongeren, Martie, and Rodríguez Llopis, Isabel

Published
2020
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9. Safe innovation approach: Towards an agile system for dealing with innovations

Author

Soeteman-Hernandez, Lya G., Apostolova, Margarita D., Bekker, Cindy, Dekkers, Susan, Grafström, Roland C., Groenewold, Monique, Handzhiyski, Yordan, Herbeck-Engel, Petra, Hoehener, Karl, Karagkiozaki, Varvara, Kelly, Sean, Kraegeloh, Annette, Logothetidis, Stergios, Micheletti, Christian, Nymark, Penny, Oomen, Agnes, Oosterwijk, Thies, Rodríguez-LLopis, Isabel, Sabella, Stefania, Sanchez Jiménez, Araceli, Sips, Adriënne J.A.M., Suarez- Merino, Blanca, Tavernaro, Isabella, van Engelen, Jacqueline, Wijnhoven, Susan W.P., and Noorlander, Cornelle W.

Published
2019
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10. Nanomaterial grouping: Existing approaches and future recommendations

Author

Giusti, Anna, Atluri, Rambabu, Tsekovska, Rositsa, Gajewicz, Agnieszka, Apostolova, Margarita D., Battistelli, Chiara L., Bleeker, Eric A.J., Bossa, Cecilia, Bouillard, Jacques, Dusinska, Maria, Gómez-Fernández, Paloma, Grafström, Roland, Gromelski, Maciej, Handzhiyski, Yordan, Jacobsen, Nicklas Raun, Jantunen, Paula, Jensen, Keld Alstrup, Mech, Agnieszka, Navas, José Maria, Nymark, Penny, Oomen, Agnes G., Puzyn, Tomasz, Rasmussen, Kirsten, Riebeling, Christian, Rodriguez-Llopis, Isabel, Sabella, Stefania, Sintes, Juan Riego, Suarez-Merino, Blanca, Tanasescu, Speranta, Wallin, Håkan, and Haase, Andrea

Published
2019
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14. Surface Modification of Additively Fabricated Titanium-Based Implants by Means of Bioactive Micro-Arc Oxidation Coatings for Bone Replacement

Author

Kozelskaya, Anna I., primary, Rutkowski, Sven, additional, Frueh, Johannes, additional, Gogolev, Aleksey S., additional, Chistyakov, Sergei G., additional, Gnedenkov, Sergey V., additional, Sinebryukhov, Sergey L., additional, Frueh, Andreas, additional, Egorkin, Vladimir S., additional, Choynzonov, Evgeny L., additional, Buldakov, Mikhail, additional, Kulbakin, Denis E., additional, Bolbasov, Evgeny N., additional, Gryaznov, Anton P., additional, Verzunova, Ksenia N., additional, Apostolova, Margarita D., additional, and Tverdokhlebov, Sergei I., additional

Published
2022
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15. Towards FAIR nanosafety data

Author

European Commission, Jeliazkova, Nina [0000-0002-4322-6179], Apostolova, Margarita D. [0000-0001-7188-506X], Andreoli, Cristina [0000-0003-3724-0668], Barone, Flavia [0000-0002-8929-7749], Barrick, Andrew [0000-0002-2653-2780], Battistelli, Chiara [0000-0003-2386-0727], Bossa, Cecilia [0000-0003-2084-2902], Châtel, Amélie [0000-0003-2245-0216], De Angelis, Isabella [0000-0001-7820-3631], Dusinska, Maria [0000-0003-1358-1652], El Yamani, Naouale [0000-0001-5456-8230], Gheorghe, Daniela [0000-0002-3835-7318], Giusti, Anna [0000-0002-5027-2069], Gómez-Fernández, Paloma [0000-0002-2086-0652], Jeliazkov, Vedrin [0000-0002-1520-5995], Jensen, Keld Alstrup [0000-0002-2764-3634], Gromelski, Maciej [0000-0001-5185-8208], Jacobsen, Nicklas Raun [0000-0002-2504-2229], Kochev, Nikolay [0000-0001-6547-3675], Kohonen, Pekka [0000-0002-7845-3611], Manier, Nicolas [0000-0003-3873-6657], Mariussen, Espen [0000-0001-5150-942X], Navas Antón, José María [0000-0002-7644-8499], Paskaleva, Vesselina [0000-0002-0560-4511], Precupas, Aurica [0000-0002-2381-7617], Puzyn, Tomasz [0000-0003-0449-8339], Llopis, Isabel Rodríguez [0000-0003-1514-4800], Rundén-Pran, Elise [0000-0002-5541-4513], Sandu, Romica [0000-0003-3449-5905], Shandilya, Neeraj [0000-0003-3328-8517], Tanasescu, Speranta [0000-0002-4326-1839], Nymark, Penny [0000-0002-3435-7775], Jeliazkova, Nina, Apostolova, Margarita D, Andreoli, Cristina, Barone, Flavia, Barrick, Andrew, Battistelli, Chiara, Bossa, Cecilia, Botea-Petcu, Alina, Châtel, Amélie, De Angelis, Isabella, Dusinska, Maria, El Yamani, Naouale, Gheorghe, Daniela, Giusti, Anna, Gómez-Fernández, Paloma, Gromelski, Maciej, Jacobsen, Nicklas Raun, Jeliazkov, Vedrin, Jensen, Keld Alstrup, Kochev, Nikolay, Kohonen, Pekka, Manier, Nicolas, Mariussen, Espen, Mech, Agnieszka, Navas Antón, José María, Paskaleva, Vesselina, Precupas, Aurica, Puzyn, Tomasz, Rasmussen, Kirsten, Ritchie, Peter, Llopis, Isabel Rodríguez, Rundén-Pran, Elise, Sandu, Romica, Shandilya, Neeraj, Tanasescu, Speranta, Haase, Andrea, Nymark, Penny, European Commission, Jeliazkova, Nina [0000-0002-4322-6179], Apostolova, Margarita D. [0000-0001-7188-506X], Andreoli, Cristina [0000-0003-3724-0668], Barone, Flavia [0000-0002-8929-7749], Barrick, Andrew [0000-0002-2653-2780], Battistelli, Chiara [0000-0003-2386-0727], Bossa, Cecilia [0000-0003-2084-2902], Châtel, Amélie [0000-0003-2245-0216], De Angelis, Isabella [0000-0001-7820-3631], Dusinska, Maria [0000-0003-1358-1652], El Yamani, Naouale [0000-0001-5456-8230], Gheorghe, Daniela [0000-0002-3835-7318], Giusti, Anna [0000-0002-5027-2069], Gómez-Fernández, Paloma [0000-0002-2086-0652], Jeliazkov, Vedrin [0000-0002-1520-5995], Jensen, Keld Alstrup [0000-0002-2764-3634], Gromelski, Maciej [0000-0001-5185-8208], Jacobsen, Nicklas Raun [0000-0002-2504-2229], Kochev, Nikolay [0000-0001-6547-3675], Kohonen, Pekka [0000-0002-7845-3611], Manier, Nicolas [0000-0003-3873-6657], Mariussen, Espen [0000-0001-5150-942X], Navas Antón, José María [0000-0002-7644-8499], Paskaleva, Vesselina [0000-0002-0560-4511], Precupas, Aurica [0000-0002-2381-7617], Puzyn, Tomasz [0000-0003-0449-8339], Llopis, Isabel Rodríguez [0000-0003-1514-4800], Rundén-Pran, Elise [0000-0002-5541-4513], Sandu, Romica [0000-0003-3449-5905], Shandilya, Neeraj [0000-0003-3328-8517], Tanasescu, Speranta [0000-0002-4326-1839], Nymark, Penny [0000-0002-3435-7775], Jeliazkova, Nina, Apostolova, Margarita D, Andreoli, Cristina, Barone, Flavia, Barrick, Andrew, Battistelli, Chiara, Bossa, Cecilia, Botea-Petcu, Alina, Châtel, Amélie, De Angelis, Isabella, Dusinska, Maria, El Yamani, Naouale, Gheorghe, Daniela, Giusti, Anna, Gómez-Fernández, Paloma, Gromelski, Maciej, Jacobsen, Nicklas Raun, Jeliazkov, Vedrin, Jensen, Keld Alstrup, Kochev, Nikolay, Kohonen, Pekka, Manier, Nicolas, Mariussen, Espen, Mech, Agnieszka, Navas Antón, José María, Paskaleva, Vesselina, Precupas, Aurica, Puzyn, Tomasz, Rasmussen, Kirsten, Ritchie, Peter, Llopis, Isabel Rodríguez, Rundén-Pran, Elise, Sandu, Romica, Shandilya, Neeraj, Tanasescu, Speranta, Haase, Andrea, and Nymark, Penny

Abstract

Nanotechnology is a key enabling technology with billions of euros in global investment from public funding, which include large collaborative projects that have investigated environmental and health safety aspects of nanomaterials, but the reuse of accumulated data is clearly lagging behind. Here we summarize challenges and provide recommendations for the efficient reuse of nanosafety data, in line with the recently established FAIR (findable, accessible, interoperable and reusable) guiding principles. We describe the FAIR-aligned Nanosafety Data Interface, with an aggregated findability, accessibility and interoperability across physicochemical, bio-nano interaction, human toxicity, omics, ecotoxicological and exposure data. Overall, we illustrate a much-needed path towards standards for the optimized use of existing data, which avoids duplication of efforts, and provides a multitude of options to promote safe and sustainable nanotechnology.

Published
2021

17. Safe(r) by design implementation in the nanotechnology industry

Author

European Commission, Sánchez-Jiménez, A. [0000-0002-2625-9155], Puelles, Raquel [0000-0002-7185-2177], Pérez-Fernández, Marta [0000-0002-8522-6679], Gómez-Fernández, Paloma [0000-0002-2086-0652], Barruetabeña, Leire [0000-0003-4648-9959], Jacobsen, Nicklas Raun [0000-0002-2504-2229], Suarez-Merino, Blanca [0000-0002-0201-265X], Manier, Nicolas [0000-0003-3873-6657], Trouiller, Bénédicte [0000-0003-4367-2870], Navas Antón, José María [0000-0002-7644-8499], Kalman, Judit [0000-0002-3610-1968], Salieri, Beatrice [0000-0002-8338-6206], Hischier, Roland [0000-0002-1084-7665], Handzhiyski, Yordan [0000-0002-8248-0524], Apostolova, Margarita D [0000-0001-7188-506X], Hadrup, Niels [0000-0002-1188-445X], Oudart, Yohan [0000-0001-9790-0766], Merino, Cesar [0000-0002-1414-2208], Liguori, Biase [0000-0001-9531-7796], Sabella, Stefania [0000-0003-3353-3607], Rose, Jerome [0000-0003-3071-8147], Galea, Karen S. [0000-0002-9540-8513], Stepankova, Sandra [0000-0002-9730-7302], Mouneyrac, Catherine [0000-0003-3769-0386], Barrick, Andrew [0000-0002-2653-2780], Châtel, Amélie [0000-0003-2245-0216], Dusinska, Maria [0000-0003-1358-1652], Rundén-Pran, Elise [0000-0002-5541-4513], Mariussen, Espen [0000-0001-5150-942X], Bressot, Christophe [0000-0002-3649-0596], Aguerre-Chariol, Olivier [0000-0001-9488-9530], Shandilya, Neeraj [0000-0003-3328-8517], Gomez-Cordon, Julio [0000-0002-6749-4354], Simar, Sophie [0000-0002-9144-5149], Nesslany, Fabrice [0000-0003-2846-1442], Jensen, Keld Alstrup [0000-0002-2764-3634], van Tongeren, Martie [0000-0002-1205-1898], Rodríguez Llopis, Isabel [0000-0003-1514-4800], Sánchez-Jiménez, A., Puelles, Raquel, Pérez-Fernández, Marta, Gómez-Fernández, Paloma, Barruetabeña, Leire, Jacobsen, Nicklas Raun, Suarez-Merino, Blanca, Micheletti, Christian, Manier, Nicolas, Trouiller, Bénédicte, Navas Antón, José María, Kalman, Judit, Salieri, Beatrice, Hischier, Roland, Handzhiyski, Yordan, Apostolova, Margarita D, Hadrup, Niels, Bouillard, Jacques, Oudart, Yohan, Merino, Cesar, Garcia, Erika, Liguori, Biase, Sabella, Stefania, Rose, Jerome, Masion, Armand, Galea, Karen S., Kelly, Sean, Stepankova, Sandra, Mouneyrac, Catherine, Barrick, Andrew, Châtel, Amélie, Dusinska, Maria, Rundén-Pran, Elise, Mariussen, Espen, Bressot, Christophe, Aguerre-Chariol, Olivier, Shandilya, Neeraj, Goede, Henk, Gomez-Cordon, Julio, Simar, Sophie, Nesslany, Fabrice, Jensen, Keld Alstrup, van Tongeren, Martie, Rodríguez Llopis, Isabel, European Commission, Sánchez-Jiménez, A. [0000-0002-2625-9155], Puelles, Raquel [0000-0002-7185-2177], Pérez-Fernández, Marta [0000-0002-8522-6679], Gómez-Fernández, Paloma [0000-0002-2086-0652], Barruetabeña, Leire [0000-0003-4648-9959], Jacobsen, Nicklas Raun [0000-0002-2504-2229], Suarez-Merino, Blanca [0000-0002-0201-265X], Manier, Nicolas [0000-0003-3873-6657], Trouiller, Bénédicte [0000-0003-4367-2870], Navas Antón, José María [0000-0002-7644-8499], Kalman, Judit [0000-0002-3610-1968], Salieri, Beatrice [0000-0002-8338-6206], Hischier, Roland [0000-0002-1084-7665], Handzhiyski, Yordan [0000-0002-8248-0524], Apostolova, Margarita D [0000-0001-7188-506X], Hadrup, Niels [0000-0002-1188-445X], Oudart, Yohan [0000-0001-9790-0766], Merino, Cesar [0000-0002-1414-2208], Liguori, Biase [0000-0001-9531-7796], Sabella, Stefania [0000-0003-3353-3607], Rose, Jerome [0000-0003-3071-8147], Galea, Karen S. [0000-0002-9540-8513], Stepankova, Sandra [0000-0002-9730-7302], Mouneyrac, Catherine [0000-0003-3769-0386], Barrick, Andrew [0000-0002-2653-2780], Châtel, Amélie [0000-0003-2245-0216], Dusinska, Maria [0000-0003-1358-1652], Rundén-Pran, Elise [0000-0002-5541-4513], Mariussen, Espen [0000-0001-5150-942X], Bressot, Christophe [0000-0002-3649-0596], Aguerre-Chariol, Olivier [0000-0001-9488-9530], Shandilya, Neeraj [0000-0003-3328-8517], Gomez-Cordon, Julio [0000-0002-6749-4354], Simar, Sophie [0000-0002-9144-5149], Nesslany, Fabrice [0000-0003-2846-1442], Jensen, Keld Alstrup [0000-0002-2764-3634], van Tongeren, Martie [0000-0002-1205-1898], Rodríguez Llopis, Isabel [0000-0003-1514-4800], Sánchez-Jiménez, A., Puelles, Raquel, Pérez-Fernández, Marta, Gómez-Fernández, Paloma, Barruetabeña, Leire, Jacobsen, Nicklas Raun, Suarez-Merino, Blanca, Micheletti, Christian, Manier, Nicolas, Trouiller, Bénédicte, Navas Antón, José María, Kalman, Judit, Salieri, Beatrice, Hischier, Roland, Handzhiyski, Yordan, Apostolova, Margarita D, Hadrup, Niels, Bouillard, Jacques, Oudart, Yohan, Merino, Cesar, Garcia, Erika, Liguori, Biase, Sabella, Stefania, Rose, Jerome, Masion, Armand, Galea, Karen S., Kelly, Sean, Stepankova, Sandra, Mouneyrac, Catherine, Barrick, Andrew, Châtel, Amélie, Dusinska, Maria, Rundén-Pran, Elise, Mariussen, Espen, Bressot, Christophe, Aguerre-Chariol, Olivier, Shandilya, Neeraj, Goede, Henk, Gomez-Cordon, Julio, Simar, Sophie, Nesslany, Fabrice, Jensen, Keld Alstrup, van Tongeren, Martie, and Rodríguez Llopis, Isabel

Abstract

The implementation of Safe(r) by Design (SbD) in industrial innovations requires an integrated approach where the human, environmental and economic impact of the SbD measures is evaluated across and throughout the nanomaterial (NM) life cycle. SbD was implemented in six industrial companies where SbD measures were applied to NMs, nano-enabled products (NEPs) and NM/NEP manufacturing processes. The approach considers human and environmental risks, functionality of the NM/NEP and costs as early as possible in the innovation process, continuing throughout the innovation progresses. Based on the results of the evaluation, a decision has to be made on whether to continue, stop or re-design the NM/NEP/process or to carry out further tests/obtain further data in cases where the uncertainty of the human and environmental risks is too large. However, SbD can also be implemented at later stages when there is already a prototype product or process available, as demonstrated in some of the cases. The SbD measures implemented in some of the case studies did not result in a viable solution. For example the coating of silicon nanoparticles with amorphous carbon increased the conductivity, the stability and reduced the dustiness of the particles and therefore the risk of explosion and the exposure to workers. However the socioeconomic assessment for their use in lithium-ion batteries for cars, when compared to the use of graphite, showed that the increase in performance did not overcome the higher production costs. This work illustrates the complexities of selecting the most appropriate SbD measures and highlights that SbD cannot be solely based on a hazard and exposure assessment but must include other impacts that any SbD measures may have on sustainability including energy consumption and waste generation as well as all associated monetary costs.

Published
2020

19. Safe(r) by design guidelines for the nanotechnology industry

Author

Sánchez Jiménez, Araceli, primary, Puelles, Raquel, additional, Perez-Fernandez, Marta, additional, Barruetabeña, Leire, additional, Jacobsen, Nicklas Raun, additional, Suarez-Merino, Blanca, additional, Micheletti, Christian, additional, Manier, Nicolas, additional, Salieri, Beatrice, additional, Hischier, Roland, additional, Tsekovska, Rositsa, additional, Handzhiyski, Yordan, additional, Bouillard, Jacques, additional, Oudart, Yohan, additional, Galea, Karen S., additional, Kelly, Sean, additional, Shandilya, Neeraj, additional, Goede, Henk, additional, Gomez-Cordon, Julio, additional, Jensen, Keld Alstrup, additional, van Tongeren, Martie, additional, Apostolova, Margarita D., additional, and Llopis, Isabel Rodríguez, additional

Published
2022
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21. Effect of conventional and more aggressive rosuvastatin treatment on markers of endothelial activation

Author

Postadzhiyan Arman S., Tzontcheva Anna V., Kehajov Ivan, Kyurkchiev Stanimir, Apostolova Margarita D., and Finkov Bojidar

Subjects
soluble adhesion molecules, statins, acute coronary syndrome, inflammation, Biochemistry, QD415-436
Abstract

Treatment of hypercholesterolemia with HMGCoA reductase inhibitors results in an earlier reduction of morbidity and mortality than expected from trials using conventional cholesterol-lowering therapies. Possible explanations for this effect include improvement of endothelial function, plaque stabilization, and inhibition of the inflammatory response associated with atherosclerosis. In this study we assessed the effects of low and moderate dose rosuvastatin treatment, on circulating markers of endothelial activation in patients admitted for acute coronary syndromes without ST segment elevation. Thirty patients with unstable angina and non ST segment elevation myocardial infarction were randomized into two groups, and received rosuvastatin 10 mg/day (n =16) or rosuvastatin 20 mg/day (n =14) for 12 weeks. Circulating levels of soluble vascular cell adhesion molecule (sVCAM-1) and inter cellular adhesion molecule-1 (sICAM-1) were measured at admission, and at the end of the study. Lipid values were significantly reduced in both treatment groups, but with significantly greater reduction in the aggressively treated group. Serum levels of sICAM-1 and sVCAM- 1 were significantly decreased in both rosuvastatin-treated groups during 12 weeks of follow-up with a more pronounced decrease in the more aggressively treated group. Therefore, rosuvastatin modulates endothelial activation in patients after acute coronary syndromes.

Published
2008

22. Advances in Multifunctional Bioactive Coatings for Metallic Bone Implants.

Author

Nikolova, Maria P. and Apostolova, Margarita D.

Subjects
*METAL coating, *BIOMEDICAL materials, *BIOABSORBABLE implants, *GROWTH factors, *CORROSION resistance
Abstract

To fix the bone in orthopedics, it is almost always necessary to use implants. Metals provide the needed physical and mechanical properties for load-bearing applications. Although widely used as biomedical materials for the replacement of hard tissue, metallic implants still confront challenges, among which the foremost is their low biocompatibility. Some of them also suffer from excessive wear, low corrosion resistance, infections and shielding stress. To address these issues, various coatings have been applied to enhance their in vitro and in vivo performance. When merged with the beneficial properties of various bio-ceramic or polymer coatings remarkable bioactive, osteogenic, antibacterial, or biodegradable composite implants can be created. In this review, bioactive and high-performance coatings for metallic bone implants are systematically reviewed and their biocompatibility is discussed. Updates in coating materials and formulations for metallic implants, as well as their production routes, have been provided. The ways of improving the bioactive coating performance by incorporating bioactive moieties such as growth factors, osteogenic factors, immunomodulatory factors, antibiotics, or other drugs that are locally released in a controlled manner have also been addressed. [ABSTRACT FROM AUTHOR]

Published
2023
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32. High-glucose-induced metallothionein expression in endothelial cells: an endothelin-mediated mechanism

Author

APOSTOLOVA, MARGARITA D., CHEN, SHALI, CHAKRABARTI, SUBRATA, and CHERIAN, M. GEORGE

Subjects
Diabetes -- Physiological aspects, Oxidation, Physiological -- Research, Cell research -- Analysis, Biological sciences
Abstract

Vascular endothelial cells are constantly exposed to oxidative stress and must be protected by physiological responses. In diabetes mellitus, endothelial cell permeability is impaired and may be increased by high extracellular glucose concentrations. It has been postulated that metallothionein (MT) can protect endothelial cells from oxidative stress with its increased expression by cytokines, thrombin, and endothelin (ET)-1. In this study, we demonstrate that high glucose concentration can induce MT expression in endothelial cells through a distinct ET-dependent pathway. Exposure of human umbilical vein endothelial cells (HUVEC) to increasing concentrations of glucose resulted in a rapid dose-dependent increase in MT-2 and ET-1 mRNA expression. MT expression may be further augmented with addition of ET-1. Preincubation of the cells with the specific [ET.sub.B] antagonist BQ-788 blocked MT-2 mRNA expression more effectively than the [ET.sub.A] inhibitor TBC-11251. High glucose also increased immunoreactive MT protein expression and induced translocation of MT into the perinuclear area. Perinuclear localization of MT was related to high-glucose-induced reorganization of F-actin filaments. These results demonstrate that an increase in extracellular glucose in HUVEC can lead to a rapid dose-dependent increase in MT-2 mRNA expression and to perinuclear localization of MT protein with changes to the cytoskeleton. These effects are mediated via the ET receptor-dependent pathway. human umbilical vein endothelial cells; TBC-11251; BQ-788

Published
2001

44. New Heterocyclic Combretastatin A-4 Analogs: Synthesis and Biological Activity of Styryl-2(3 H)-benzothiazolones.

Author

Atanasov, Gjorgji, Rusew, Rusi I., Gelev, Vladimir M., Chanev, Christo D., Nikolova, Rosica, Shivachev, Boris L., Petrov, Ognyan I., and Apostolova, Margarita D.

Subjects
BIOSYNTHESIS, SPINDLE apparatus, ENDOTHELIAL cells, CELL death, NEOVASCULARIZATION, CYTOKINESIS, CELL cycle
Abstract

Here, we describe the synthesis, characterization, and biological activities of a series of 26 new styryl-2(3H)-benzothiazolone analogs of combretastatin-A4 (CA-4). The cytotoxic activities of these compounds were tested in several cell lines (EA.hy926, A549, BEAS-2B, MDA-MB-231, HT-29, MCF-7, and MCF-10A), and the relations between structure and cytotoxicity are discussed. From the series, compound (Z)-3-methyl-6-(3,4,5-trimethoxystyryl)-2(3H)-benzothiazolone (26Z) exhibits the most potent cytotoxic activity (IC50 0.13 ± 0.01 µM) against EA.hy926 cells. 26Z not only inhibits vasculogenesis but also disrupts pre-existing vasculature. 26Z is a microtubule-modulating agent and inhibits a spectrum of angiogenic events in EA.hy926 cells by interfering with endothelial cell invasion, migration, and proliferation. 26Z also shows anti-proliferative activity in CA-4 resistant cells with the following IC50 values: HT-29 (0.008 ± 0.001 µM), MDA-MB-231 (1.35 ± 0.42 µM), and MCF-7 (2.42 ± 0.48 µM). Cell-cycle phase-specific experiments show that 26Z treatment results in G2/M arrest and mitotic spindle multipolarity, suggesting that drug-induced centrosome amplification could promote cell death. Some 26Z-treated adherent cells undergo aberrant cytokinesis, resulting in aneuploidy that perhaps contributes to drug-induced cell death. These data indicate that spindle multipolarity induction by 26Z has an exciting chemotherapeutic potential that merits further investigation. [ABSTRACT FROM AUTHOR]

Published
2021
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45. Application of safe-by-design approach for curing osteoporosis -- a lock at the future.

Author

Grigoryan, Armine, Kolev, Iliyan, Dimitrova, Anelia, and Apostolova, Margarita D.

Subjects
OSTEOPOROSIS, BONE regeneration, STEM cell transplantation, PROGENITOR cells, OSTEOBLASTS, CELL differentiation
Abstract

During the last years several studies have been performed to develop new materials for stimulation of bone healing and regeneration. Whilst it is known that osteoporotic bone is generally not cancellous in nature and has thin walls, the essential design paradigm of implants does not reflect this application. We have proposed a simple system for assisted bone repair by in bone injection of "intelligent" polymers combined with nanodiamonds and progenitor cells. A Safe-by-Design approach was undertaken. It utilizes autologous stem cells transplantation in combination with supportive bioresorbable matrices and bioactive molecules for enhancing growth and repair. The endothelial progenitor cells obtained from peripheral blood of the same patient may be cultured in vitro in the presence of different stimuli and/or nanoparticles to undergo osteoblasts differentiation, prior to autologous transplantation. This injectable therapy could also be used for: (1) modifying the bone interior morphology, porosity and interconnectivity, which are extremely important for cell adhesion, proliferation and differentiation; (2) prophylactic treatment for high risk patients to prevent fractures, especially the hip and vertebrae; (3) providing exceptional repair of the osteoporotic bone by releasing pharmaceuticals to the specific sites with the purpose of accelerating healing, promoting angiogenesis, reducing the risk of infection, etc. [ABSTRACT FROM AUTHOR]

Published
2017
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46. Towards FAIR nanosafety data

Author

Vesselina Paskaleva, Nicolas Manier, Vedrin Jeliazkov, Penny Nymark, Keld Alstrup Jensen, Peter Ritchie, Maria Dusinska, Nicklas Raun Jacobsen, Pekka Kohonen, Margarita D. Apostolova, Nikolay Kochev, Elise Rundén-Pran, Isabel Rodríguez Llopis, Agnieszka Mech, Naouale El Yamani, Andrea Haase, Cristina Andreoli, Isabella De Angelis, Speranta Tanasescu, Aurica Precupas, Flavia Barone, Chiara Laura Battistelli, Kirsten Rasmussen, Tomasz Puzyn, José María Navas, Roland C. Grafström, Daniela Gheorghe, Cecilia Bossa, Maciej Gromelski, Paloma Gómez-Fernández, Espen Mariussen, Alina Botea-Petcu, Anna Giusti, Andrew Barrick, Romica Sandu, Neeraj Shandilya, Nina Jeliazkova, Amélie Châtel, European Commission, Jeliazkova, Nina, Apostolova, Margarita D., Andreoli, Cristina, Barone, Flavia, Barrick, Andrew, Battistelli, Chiara, Bossa, Cecilia, Châtel, Amélie, De Angelis, Isabella, Dusinska, Maria, El Yamani, Naouale, Gheorghe, Daniela, Giusti, Anna, Gómez-Fernández, Paloma, Jeliazkov, Vedrin, Jensen, Keld Alstrup, Gromelski, Maciej, Jacobsen, Nicklas Raun, Kochev, Nikolay, Kohonen, Pekka, Manier, Nicolas, Mariussen, Espen, Navas, José María, Paskaleva, Vesselina, Precupas, Aurica, Puzyn, Tomasz, Llopis, Isabel Rodríguez, Rundén-Pran, Elise, Sandu, Romica, Shandilya, Neeraj, Tanasescu, Speranta, Nymark, Penny, Institut National de l'Environnement Industriel et des Risques (INERIS), and The Netherlands Organisation for Applied Scientific Research (TNO)

Subjects
Human toxicity, Guiding Principles, Computer science, Global investments, Interoperability, Biomedical Engineering, Bioengineering, 02 engineering and technology, Reuse, 010402 general chemistry, Collaborative projects, Ecotoxicological, 01 natural sciences, Sustainable nanotechnologies, Enabling technologies, General Materials Science, Electrical and Electronic Engineering, Lagging, 9. Industry and infrastructure, Guiding principles, Findability, 021001 nanoscience & nanotechnology, Condensed Matter Physics, Data science, Atomic and Molecular Physics, and Optics, 0104 chemical sciences, [SDE]Environmental Sciences, Key (cryptography), Data interfaces, 0210 nano-technology, Exposure data
Abstract

13 Pág. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Nanotechnology is a key enabling technology with billions of euros in global investment from public funding, which include large collaborative projects that have investigated environmental and health safety aspects of nanomaterials, but the reuse of accumulated data is clearly lagging behind. Here we summarize challenges and provide recommendations for the efficient reuse of nanosafety data, in line with the recently established FAIR (findable, accessible, interoperable and reusable) guiding principles. We describe the FAIR-aligned Nanosafety Data Interface, with an aggregated findability, accessibility and interoperability across physicochemical, bio-nano interaction, human toxicity, omics, ecotoxicological and exposure data. Overall, we illustrate a much-needed path towards standards for the optimized use of existing data, which avoids duplication of efforts, and provides a multitude of options to promote safe and sustainable nanotechnology., The work leading to this article has received funding from the European Union’s Horizon 2020 Research and Innovation programme, Grant Agreements no. 646221 (NanoReg2, 2015–2019), no. 814401 (Gov4Nano, 2019–2022) and no. 814425 (RiskGONE 2019–2023). In addition, the European Union’s 7th Framework Programme projects NANoREG (2013–2017, Grant Agreement no. 310584), NanoTEST (2008–2012, no. 201335) and ENPRA (2009–2012, no. 228789), the European Union’s Health Programme Joint Action project NANOGENOTOX (2010–2013, no. 2009 21 01) and the US NIH NCI caNanoLab portal are acknowledged for providing data

Published
2021

47. Predictive factors for high brain (B-type) natriuretic peptide at discharge in properly treated heart failure patients.

Author

Vitlianova KD, Donova TI, and Apostolova MD

Subjects
Aged, Biomarkers blood, Chi-Square Distribution, Comorbidity, Enzyme-Linked Immunosorbent Assay, Female, Humans, Linear Models, Male, Middle Aged, Patient Discharge, Practice Guidelines as Topic, Predictive Value of Tests, Prognosis, Risk Factors, Treatment Outcome, Heart Failure blood, Heart Failure therapy, Natriuretic Peptide, Brain blood
Abstract

Aim: To study differences and prognostic effect of some factors on brain (B-type) natriuretic peptide (BNP) levels at discharge of patients with chronic heart failure (CHF) treated in accord with current treatment guidelines., Patients and Methods: Eighty-five consecutive patients hospitalized for CHF were recruited into the study. A standardized study protocol was used for them including collection of blood samples for measurement of electrolytes, creatinine at baseline and BNP at discharge. High BNP levels were determined at values above the threshold value for the highest BNP quartile (> or = 463 pg/ml). Linear regression analyses were performed using the SPSS 16.0., Results: High BNP levels at discharge were measured in 21 (24.7%) of the patients. Patients with persisting high BNP levels, despite the administered correct therapy, had significantly more frequently worse clinical and instrumental characteristics: pulmonary congestion (76.2% vs. 40.6%), IV NYHA functional class (23.8% vs. 4.7%), atrial fibrillation (AF) (71.4% vs. 35.9%) and ischemic etiology of HF (47.6% vs. 15.6%). Multivariate linear regression analysis (F = 7.1, p < 0.001) identified systolic blood pressure (SBP), AF and instrumental data for pulmonary congestion as significant and independent predictors of high BNP at discharge., Conclusions: There were statistically significant differences in the distribution and prognostic effect of the studied factors across the BNP levels. CHF patients with hypotension, AF and instrumental evidence for pulmonary congestion at admission are at higher risk of high BNP levels at discharge and require special care and clinical approach.

Published
2011
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48. (4-Carbamoylphen-yl)boronic acid.

Author

Apostolova MD, Nikolova RP, and Shivachev BL

Abstract

In the title compound, C(7)H(8)BNO(3), the mol-ecule lies on an inversion center leading to a statistical disorder of the B(OH)(2) and CONH(2) groups. In the crystal structure, mol-ecules are linked by N-H⋯O and O-H⋯O hydrogen bonds, forming sheets parallel to the bc plane. The B(OH)(2) and CONH(2) groups are twisted out of the mean plane of the benzene ring by 23.9 (5) and 24.6 (6)°, respectively.

Published
2010
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