Search

Your search keyword '"Apte, Minoti V."' showing total 299 results
Start Over Author "Apte, Minoti V."
299 results on '"Apte, Minoti V."'

3. The Combination of Alcohol and Cigarette Smoke Induces Endoplasmic Reticulum Stress and Cell Death in Pancreatic Acinar Cells

Author

Lugea, Aurelia, Gerloff, Andreas, Su, Hsin-Yuan, Xu, Zhihong, Go, Ariel, Hu, Cheng, French, Samuel W, Wilson, Jeremy S, Apte, Minoti V, Waldron, Richard T, and Pandol, Stephen J

Subjects
Genetics, Digestive Diseases, Alcoholism, Alcohol Use and Health, Substance Misuse, Tobacco, Tobacco Smoke and Health, Prevention, 2.1 Biological and endogenous factors, Aetiology, Cancer, Oral and gastrointestinal, Good Health and Well Being, Acinar Cells, Alcohol Drinking, Animals, Apoptosis, Apoptosis Regulatory Proteins, Cell Line, Cigarette Smoking, Disease Models, Animal, Endoplasmic Reticulum Stress, Ethanol, Humans, Lipopolysaccharides, Male, Mice, Mice, Inbred C57BL, Necrosis, Oxidative Stress, Pancreas, Exocrine, Pancreatitis, Alcoholic, Rats, Sprague-Dawley, Risk Factors, Smoke, Time Factors, Tissue Culture Techniques, Unfolded Protein Response, Alcohol, Smoking, ER Stress, Pancreas, Clinical Sciences, Neurosciences, Paediatrics and Reproductive Medicine, Gastroenterology & Hepatology
Abstract

Background & aimsSmoking, an independent risk factor for pancreatitis, accelerates the development of alcoholic pancreatitis. Alcohol feeding of mice induces up-regulation of spliced X-box binding protein 1 (XBP1s), which regulates the endoplasmic reticulum (ER) unfolded protein response and promotes cell survival upon ER stress. We examined whether smoking affects the adaptive mechanisms induced by alcohol and accelerates disorders of the ER in pancreatic acinar cells.MethodsWe studied the combined effects of ethanol (EtOH) and cigarette smoke extract (CSE) on ER stress and cell death responses in mouse and human primary acini and the acinar cell line AR42J. Cells were incubated with EtOH (50 mmol/L), CSE (20-40 μg/mL), or both (CSE+EtOH), and analyzed by immunoblotting, quantitative reverse-transcription polymerase chain reaction, and cell death assays. Some cells were incubated with MKC-3946, an inhibitor of endoplasmic reticulum to nucleus signaling 1 (ERN1, also called IRE1) that blocks XBP1s formation. Male Sprague-Dawley rats were fed isocaloric amounts of an EtOH-containing (Lieber-DeCarli) or control diet for 11 weeks and exposed to cigarette smoke or room air in an exposure chamber for 2 hours each day. During the last 3 weeks, a subset of rats received intravenous injections of lipopolysaccharide (LPS, 3 mg/kg per week) to induce pancreatitis or saline (control). Pancreatic tissues were collected and analyzed by histology and immunostaining techniques.ResultsIn AR42J and primary acini, CSE+EtOH induced cell death (necrosis and apoptosis), but neither agent alone had this effect. Cell death was associated with a significant decrease in expression of XBP1s. CSE+EtOH, but neither agent alone, slightly decreased adenosine triphosphate levels in AR42J cells, but induced oxidative stress and sustained activation (phosphorylation) of eukaryotic translation initiation factor 2 alpha kinase 3 (EIF2AK3, also called PERK) and increased protein levels of DNA damage inducible transcript 3 (DDIT3, also called CHOP). CHOP regulates transcription to promote apoptosis. Incubation of AR42J or primary mouse or human acinar cells with MKC-3946 reduced expression of XBP1s, increased levels of CHOP, and induced cell death. In rats fed an EtOH diet, exposure to cigarette smoke increased ER stress in acinar cells and sensitized the pancreas to LPS-induced pathology.ConclusionsCigarette smoke promotes cell death and features of pancreatitis in EtOH-sensitized acinar cells by suppressing the adaptive unfolded protein response signaling pathway. It also activates ER stress pathways that promote acinar cell death.

Published
2017

8. Vitamin D Receptor-Mediated Stromal Reprogramming Suppresses Pancreatitis and Enhances Pancreatic Cancer Therapy

Author

Sherman, Mara H, Yu, Ruth T, Engle, Dannielle D, Ding, Ning, Atkins, Annette R, Tiriac, Herve, Collisson, Eric A, Connor, Frances, Van Dyke, Terry, Kozlov, Serguei, Martin, Philip, Tseng, Tiffany W, Dawson, David W, Donahue, Timothy R, Masamune, Atsushi, Shimosegawa, Tooru, Apte, Minoti V, Wilson, Jeremy S, Ng, Beverly, Lau, Sue Lynn, Gunton, Jenny E, Wahl, Geoffrey M, Hunter, Tony, Drebin, Jeffrey A, O’Dwyer, Peter J, Liddle, Christopher, Tuveson, David A, Downes, Michael, and Evans, Ronald M

Subjects
Biomedical and Clinical Sciences, Oncology and Carcinogenesis, Immunology, Digestive Diseases, Pancreatic Cancer, Orphan Drug, Nutrition, Cancer, Rare Diseases, 2.1 Biological and endogenous factors, Aetiology, Adenocarcinoma, Animals, Antineoplastic Agents, Calcitriol, Carcinoma, Pancreatic Ductal, Cell Line, Tumor, Disease Models, Animal, Gene Expression Profiling, Humans, Mice, Inbred C57BL, Molecular Sequence Data, Pancreatic Neoplasms, Pancreatitis, Receptors, Calcitriol, Signal Transduction, Stromal Cells, Biological Sciences, Medical and Health Sciences, Developmental Biology, Biological sciences, Biomedical and clinical sciences
Abstract

The poor clinical outcome in pancreatic ductal adenocarcinoma (PDA) is attributed to intrinsic chemoresistance and a growth-permissive tumor microenvironment. Conversion of quiescent to activated pancreatic stellate cells (PSCs) drives the severe stromal reaction that characterizes PDA. Here, we reveal that the vitamin D receptor (VDR) is expressed in stroma from human pancreatic tumors and that treatment with the VDR ligand calcipotriol markedly reduced markers of inflammation and fibrosis in pancreatitis and human tumor stroma. We show that VDR acts as a master transcriptional regulator of PSCs to reprise the quiescent state, resulting in induced stromal remodeling, increased intratumoral gemcitabine, reduced tumor volume, and a 57% increase in survival compared to chemotherapy alone. This work describes a molecular strategy through which transcriptional reprogramming of tumor stroma enables chemotherapeutic response and suggests vitamin D priming as an adjunct in PDA therapy. PAPERFLICK:

Published
2014

13. Data from Cancer-Associated Fibroblasts in Pancreatic Ductal Adenocarcinoma Determine Response to SLC7A11 Inhibition

Author

Sharbeen, George, primary, McCarroll, Joshua A., primary, Akerman, Anouschka, primary, Kopecky, Chantal, primary, Youkhana, Janet, primary, Kokkinos, John, primary, Holst, Jeff, primary, Boyer, Cyrille, primary, Erkan, Mert, primary, Goldstein, David, primary, Timpson, Paul, primary, Cox, Thomas R., primary, Pereira, Brooke A., primary, Chitty, Jessica L., primary, Fey, Sigrid K., primary, Najumudeen, Arafath K., primary, Campbell, Andrew D., primary, Sansom, Owen J., primary, Ignacio, Rosa Mistica C., primary, Naim, Stephanie, primary, Liu, Jie, primary, Russia, Nelson, primary, Lee, Julia, primary, Chou, Angela, primary, Johns, Amber, primary, Gill, Anthony J., primary, Gonzales-Aloy, Estrella, primary, Gebski, Val, primary, Guan, Yi Fang, primary, Pajic, Marina, primary, Turner, Nigel, primary, Apte, Minoti V., primary, Davis, Thomas P., primary, Morton, Jennifer P., primary, Haghighi, Koroush S., primary, Kasparian, Jorjina, primary, McLean, Benjamin J., primary, Setargew, Yordanos F., primary, and Phillips, Phoebe A., primary

Published
2023
Full Text
View/download PDF

14. Figure S2 from Cancer-Associated Fibroblasts in Pancreatic Ductal Adenocarcinoma Determine Response to SLC7A11 Inhibition

Author

Sharbeen, George, primary, McCarroll, Joshua A., primary, Akerman, Anouschka, primary, Kopecky, Chantal, primary, Youkhana, Janet, primary, Kokkinos, John, primary, Holst, Jeff, primary, Boyer, Cyrille, primary, Erkan, Mert, primary, Goldstein, David, primary, Timpson, Paul, primary, Cox, Thomas R., primary, Pereira, Brooke A., primary, Chitty, Jessica L., primary, Fey, Sigrid K., primary, Najumudeen, Arafath K., primary, Campbell, Andrew D., primary, Sansom, Owen J., primary, Ignacio, Rosa Mistica C., primary, Naim, Stephanie, primary, Liu, Jie, primary, Russia, Nelson, primary, Lee, Julia, primary, Chou, Angela, primary, Johns, Amber, primary, Gill, Anthony J., primary, Gonzales-Aloy, Estrella, primary, Gebski, Val, primary, Guan, Yi Fang, primary, Pajic, Marina, primary, Turner, Nigel, primary, Apte, Minoti V., primary, Davis, Thomas P., primary, Morton, Jennifer P., primary, Haghighi, Koroush S., primary, Kasparian, Jorjina, primary, McLean, Benjamin J., primary, Setargew, Yordanos F., primary, and Phillips, Phoebe A., primary

Published
2023
Full Text
View/download PDF

15. Supplementary Table 2 from Cancer-Associated Fibroblasts in Pancreatic Ductal Adenocarcinoma Determine Response to SLC7A11 Inhibition

Author

Sharbeen, George, primary, McCarroll, Joshua A., primary, Akerman, Anouschka, primary, Kopecky, Chantal, primary, Youkhana, Janet, primary, Kokkinos, John, primary, Holst, Jeff, primary, Boyer, Cyrille, primary, Erkan, Mert, primary, Goldstein, David, primary, Timpson, Paul, primary, Cox, Thomas R., primary, Pereira, Brooke A., primary, Chitty, Jessica L., primary, Fey, Sigrid K., primary, Najumudeen, Arafath K., primary, Campbell, Andrew D., primary, Sansom, Owen J., primary, Ignacio, Rosa Mistica C., primary, Naim, Stephanie, primary, Liu, Jie, primary, Russia, Nelson, primary, Lee, Julia, primary, Chou, Angela, primary, Johns, Amber, primary, Gill, Anthony J., primary, Gonzales-Aloy, Estrella, primary, Gebski, Val, primary, Guan, Yi Fang, primary, Pajic, Marina, primary, Turner, Nigel, primary, Apte, Minoti V., primary, Davis, Thomas P., primary, Morton, Jennifer P., primary, Haghighi, Koroush S., primary, Kasparian, Jorjina, primary, McLean, Benjamin J., primary, Setargew, Yordanos F., primary, and Phillips, Phoebe A., primary

Published
2023
Full Text
View/download PDF

16. Supplementary Table 4 from Cancer-Associated Fibroblasts in Pancreatic Ductal Adenocarcinoma Determine Response to SLC7A11 Inhibition

Author

Sharbeen, George, primary, McCarroll, Joshua A., primary, Akerman, Anouschka, primary, Kopecky, Chantal, primary, Youkhana, Janet, primary, Kokkinos, John, primary, Holst, Jeff, primary, Boyer, Cyrille, primary, Erkan, Mert, primary, Goldstein, David, primary, Timpson, Paul, primary, Cox, Thomas R., primary, Pereira, Brooke A., primary, Chitty, Jessica L., primary, Fey, Sigrid K., primary, Najumudeen, Arafath K., primary, Campbell, Andrew D., primary, Sansom, Owen J., primary, Ignacio, Rosa Mistica C., primary, Naim, Stephanie, primary, Liu, Jie, primary, Russia, Nelson, primary, Lee, Julia, primary, Chou, Angela, primary, Johns, Amber, primary, Gill, Anthony J., primary, Gonzales-Aloy, Estrella, primary, Gebski, Val, primary, Guan, Yi Fang, primary, Pajic, Marina, primary, Turner, Nigel, primary, Apte, Minoti V., primary, Davis, Thomas P., primary, Morton, Jennifer P., primary, Haghighi, Koroush S., primary, Kasparian, Jorjina, primary, McLean, Benjamin J., primary, Setargew, Yordanos F., primary, and Phillips, Phoebe A., primary

Published
2023
Full Text
View/download PDF

24. Su1261: PANCREATIC CANCER-RELATED DIABETES: A HIGH FRUCTOSE DIET FED TO TRANSGENIC, KRASLSL-G12D/+; PDXCRE/+ (KC) MICE, IS ASSOCIATED WITH PANCREATIC STELLATE CELL ACTIVATION AND ACCELERATED CANCER PROGRESSION

Author

Perera, Chamini, primary, Hossain, Md Jakir, additional, Mekapogu, Alpha Raj, additional, Hosen, S. M. Zahid, additional, Rajan, Parvathy, additional, Xu, Zhihong, additional, Greenfield, Jerry, additional, Hoehn, Kyle, additional, Horadagoda, Neil, additional, Chari, Suresh, additional, Pirola, Romano, additional, Wilson, Jeremy, additional, and Apte, Minoti V., additional

Published
2022
Full Text
View/download PDF

29. List of Contributors

Author

Apte, Minoti V., primary, Hasegawa, Daisuke, additional, Wallace, Michael C., additional, Friedman, Scott L., additional, Brenner, David A., additional, Caligiuri, Alessandra, additional, Campana, Lara, additional, Dapito, Dianne H., additional, Gandhi, Chandrashekhar R., additional, Iredale, John, additional, Kawada, Norifumi, additional, Kisseleva, Tatiana, additional, Koyama, Yukinori, additional, Lotersztajn, Sophie, additional, Mallat, Ariane, additional, Mandal, Palash, additional, Mann, Derek A., additional, Marra, Fabio, additional, Nagy, Laura E., additional, Oakley, Fiona, additional, Parola, Maurizio, additional, Pinzani, Massimo, additional, Pirola, Romano C., additional, Rockey, Don C., additional, Rombouts, Krista, additional, Schwabe, Robert F., additional, Wang, Ping, additional, and Wilson, Jeremy S., additional

Published
2015
Full Text
View/download PDF

34. Cancer-Associated Fibroblasts in Pancreatic Ductal Adenocarcinoma Determine Response to SLC7A11 Inhibition

Author

Sharbeen, George, primary, McCarroll, Joshua A., additional, Akerman, Anouschka, additional, Kopecky, Chantal, additional, Youkhana, Janet, additional, Kokkinos, John, additional, Holst, Jeff, additional, Boyer, Cyrille, additional, Erkan, Mert, additional, Goldstein, David, additional, Timpson, Paul, additional, Cox, Thomas R., additional, Pereira, Brooke A., additional, Chitty, Jessica L., additional, Fey, Sigrid K., additional, Najumudeen, Arafath K., additional, Campbell, Andrew D., additional, Sansom, Owen J., additional, Ignacio, Rosa Mistica C., additional, Naim, Stephanie, additional, Liu, Jie, additional, Russia, Nelson, additional, Lee, Julia, additional, Chou, Angela, additional, Johns, Amber, additional, Gill, Anthony J., additional, Gonzales-Aloy, Estrella, additional, Gebski, Val, additional, Guan, Yi Fang, additional, Pajic, Marina, additional, Turner, Nigel, additional, Apte, Minoti V., additional, Davis, Thomas P., additional, Morton, Jennifer P., additional, Haghighi, Koroush S., additional, Kasparian, Jorjina, additional, McLean, Benjamin J., additional, Setargew, Yordanos F., additional, and Phillips, Phoebe A., additional

Published
2021
Full Text
View/download PDF

35. 184 HEPATOCYTE GROWTH FACTOR/ C-MET PATHWAY INHIBITION COMBINED WITH CHEMOTHERAPY RETARDS PRECURSOR PANINS AND POTENTIATES LOCAL IMMUNITY IN A TRANSGENIC MODEL OF EARLY PANCREATIC CANCER

Author

Mekapogu, Alpha Raj, primary, Xu, Zhihong, additional, Pothula, Srinivasa, additional, Perera, Chamini, additional, Pang, Tony, additional, Hosen, S. M. Zahid, additional, Damalanka, Vishnu C., additional, Mahoney, Matthew, additional, Janetka, James W., additional, Klampfer, Lidija, additional, Goldstein, David, additional, Pirola, Romano, additional, Wilson, Jeremy, additional, and Apte, Minoti V., additional

Published
2021
Full Text
View/download PDF

38. Role of alcohol metabolism in chronic pancreatitis

Author

Vonlaufen, Alain, Wilson, Jeremy S., Pirola, Romano C., and Apte, Minoti V.

Subjects
Cell research -- Physiological aspects, Pancreatitis -- Physiological aspects, Health, Physiological aspects
Abstract

Alcohol abuse is the major cause of chronic inflammation of the pancreas (i.e., chronic pancreatitis). Although it has long been thought that alcoholic pancreatitis is a chronic disease from the [...]

Published
2007

39. Breaking the cycle: Targeting of NDRG1 to inhibit bi‐directional oncogenic cross‐talk between pancreatic cancer and stroma

Author

Geleta, Bekesho, primary, Park, Kyung Chan, additional, Jansson, Patric J., additional, Sahni, Sumit, additional, Maleki, Sanaz, additional, Xu, Zhihong, additional, Murakami, Takashi, additional, Pajic, Marina, additional, Apte, Minoti V., additional, Richardson, Des R., additional, and Kovacevic, Zaklina, additional

Published
2021
Full Text
View/download PDF

43. StellaTUM: current consensus and discussion on pancreatic stellate cell research

Author

Erkan, Mert, Adler, Guido, Apte, Minoti V, Bachem, Max G, Buchholz, Malte, Detlefsen, Sönke, Esposito, Irene, Friess, Helmut, Gress, Thomas M, Habisch, Hans-Joerg, Hwang, Rosa F, Jaster, Robert, Kleeff, Jörg, Klöppel, Günter, Kordes, Claus, Logsdon, Craig D, Masamune, Atsushi, Michalski, Christoph W, Oh, Junseo, Phillips, Phoebe A, Pinzani, Massimo, Reiser-Erkan, Carolin, Tsukamoto, Hidekazu, and Wilson, Jeremy

Published
2012
Full Text
View/download PDF

46. 1133 PANCREATIC STELLATE CELL AND CANCER CELL DERIVED EXOSOMES IMPAIR BETA CELL FUNCTION: IMPLICATIONS FOR PANCREATIC CANCER RELATED DIABETES.

Author

Perera, Chamini, primary, Xu, Zhihong, additional, Mekapogu, Alpha Raj, additional, Hosen, S.M. Zahid, additional, Pothula, Srinivasa, additional, Greenfield, Jerry, additional, Chari, Suresh, additional, Goldstein, David, additional, Pirola, Romano, additional, Wilson, Jeremy, additional, and Apte, Minoti V., additional

Published
2020
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results