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33 results on '"Arévalo-Gardini, Enrique"'

10. A molecular diagnostic for Moniliophthora perniciosa, the causal agent of witches' broom disease of cacao, that differentiates it from its sister taxon Moniliophthora roreri

Author

Alves da Silva, Natália Jordana, primary, Menezes Reis, Sara Pereira, additional, Diorato, Vanessa Santana, additional, Rocha, José Sátiro Almeida, additional, Barbosa, Ceslaine Santos, additional, Ciampi-Guillardi, Maisa, additional, Patrocínio, Nara Georgia R.B., additional, Niella, Givaldo Rocha, additional, Solis, Karina, additional, Peñaherrera, Sofia, additional, Manco, Maria Julia da Silva, additional, Teixeira, Glauco Antonio, additional, Arévalo-Gardini, Enrique, additional, and Gramacho, Karina Peres, additional

Published
2022
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11. Azadirachta indica y Jatropha curcas reducen el ataque de Hypsipyla grandella Zéller en Swietenia macrophylla King plantada en sistemas agroforestales

Author

Guerra-Arévalo, Héctor, primary, Celis-Espinoza, Diego Fernando, additional, Diaz-Visitación, Alfredo Iban, additional, Vásquez-Vela, Ana Lucia Milagros, additional, Arévalo-López, Luís Alberto, additional, García-Soria, Diego Gonzalo, additional, Revilla-Chávez, Jorge Manuel, additional, Abanto-Rodriguez, Carlos, additional, Arévalo-Gardini, Enrique, additional, Castillo-Torres, Dennis Del, additional, and Guerra-Arévalo, Wilson Francisco, additional

Published
2022
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12. Genome sequence and effectorome of Moniliophthora perniciosa and Moniliophthora roreri subpopulations

Author

Barbosa, Ceslaine Santos, Fonseca, Rute R. da, Batista, Thiago Mafra, Barreto, Mariana Araújo, Argolo, Caio Suzart, Carvalho, Mariana Rocha de, Amaral, Daniel Oliveira Jordão do, Silva, Edson Mário de Andrade, Arévalo-Gardini, Enrique, Hidalgo, Karina Solis, Franco, Glória Regina, Pirovani, Carlos Priminho, Micheli, Fabienne, and Gramacho, Karina Peres

Published
2018
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13. Diversity in the invasive cacao pathogen Moniliophthora roreri is shaped by agriculture

Author

Díaz‐Valderrama, Jorge R., primary, Zambrano, Ricardo, additional, Cedeño‐Amador, Sergio, additional, Córdova‐Bermejo, Ulises, additional, Casas, Gianmarco G., additional, García‐Zurita, Nilda, additional, Sánchez‐Arévalo, Juan A. J., additional, Arévalo‐Gardini, Enrique, additional, Dávila, Darío, additional, Ruiz, Juan, additional, Pinchi‐Dávila, Xiomy, additional, Quispe‐Chacón, Zarella R., additional, Chia‐Wong, Julio A., additional, Hurtado‐Gonzales, Oscar P., additional, Rodríguez‐Callañaupa, Carlos A., additional, Maldonado‐Fuentes, Casto, additional, Pérez‐Callizaya, Eucebio, additional, Leiva‐Espinoza, Santos, additional, Oliva‐Cruz, Manuel, additional, Guevara‐Ruiz, Dylan M., additional, Torres‐Rojas, Esperanza, additional, Johnson, Elizabeth, additional, Phillips‐Mora, Wilbert, additional, and Aime, M. Catherine, additional

Published
2022
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16. Sudden death of Theobroma cacao L. caused by Verticillium dahliae Kleb. in Peru and its invitrobiocontrol

Author

Leon-Ttacca, Betsabe, Arévalo-Gardini, Enrique, and Bouchon, Anne Sophie

Subjects
Trichoderma, lcsh:Agriculture, fungi, lcsh:S, food and beverages, biological control, wilt, lcsh:Animal culture, lcsh:Agriculture (General), endophyte, lcsh:S1-972, lcsh:SF1-1100
Abstract

In the Atusparia sector, Tocache, of the San Martín region in Peru, two-year-old plants of Theobroma cacao L. (Malvaceae) of the CCN-51 clone showed for the first time in January 2010, symptoms of leaf yellowing, wilt and dieback, with an internal obstruction of the vascular system of the plant. To isolate the fungus, diseased tissues were cultured in Petri dishes containing potato dextrose agar oxytetracycline, and the identification was based on morphological and microscopic analyses and by the Sanger sequencing. Pathogenicity tests were carried out on three-months-old T. cacao ICS-1 clone plants and were inoculated with a conidia suspension of 1x107 cfu/mL employing two inoculation methods (Drench to the soil and stem puncture). Likewise, the antagonistic capacity (mycoparasitism and antibiosis) was established in vitro with endophytic Trichoderma isolates on the pathogen. With both inoculation methods symptoms of wilt of different degrees of severity were observed and the causal agent of sudden death in T. cacao was confirmed to be the fungus Verticillium dahliae Kleb. blastsequence analysis revealed that the isolate showed 100 % homology with V. dahliae sequences in GenBank. Trichoderma TE-91 was identified as the most aggressive mycoparasite because its metabolites inhibited completely the mycelial growth of the pathogen.

Published
2019

27. Genome sequence and effectorome of <italic>Moniliophthora perniciosa</italic> and <italic>Moniliophthora roreri</italic> subpopulations.

Author

Barbosa, Ceslaine Santos, Fonseca, Rute R. da, Batista, Thiago Mafra, Barreto, Mariana Araújo, Argolo, Caio Suzart, Carvalho, Mariana Rocha de, Amaral, Daniel Oliveira Jordão do, Silva, Edson Mário de Andrade, Arévalo-Gardini, Enrique, Hidalgo, Karina Solis, Franco, Glória Regina, Pirovani, Carlos Priminho, Micheli, Fabienne, and Gramacho, Karina Peres

Subjects
PHYTOPATHOGENIC microorganisms, CACAO diseases & pests, WITCHES' broom disease, NUCLEOTIDE sequence, PLANT genetics
Abstract

Background: The hemibiotrophic pathogens Moniliophthora perniciosa (witches' broom disease) and Moniliophthora roreri (frosty pod rot disease) are among the most important pathogens of cacao. Moniliophthora perniciosa has a broad host range and infects a variety of meristematic tissues in cacao plants, whereas M. roreri infects only pods of Theobroma and Herrania genera. Comparative pathogenomics of these fungi is essential to understand Moniliophthora infection strategies, therefore the detection and in silico functional characterization of effector candidates are important steps to gain insight on their pathogenicity. Results: Candidate secreted effector proteins repertoire were predicted using the genomes of five representative isolates of M. perniciosa subpopulations (three from cacao and two from solanaceous hosts), and one representative isolate of M. roreri from Peru. Many putative effectors candidates were identified in M. perniciosa: 157 and 134 in cacao isolates from Bahia, Brazil; 109 in cacao isolate from Ecuador, 92 and 80 in wild solanaceous isolates from Minas Gerais (Lobeira) and Bahia (Caiçara), Brazil; respectively. Moniliophthora roreri showed the highest number of effector candidates, a total of 243. A set of eight core effectors were shared among all Moniliophthora isolates, while others were shared either between the wild solanaceous isolates or among cacao isolates. Mostly, candidate effectors of M. perniciosa were shared among the isolates, whereas in M. roreri nearly 50% were exclusive to the specie. In addition, a large number of cell wall-degrading enzymes characteristic of hemibiotrophic fungi were found. From these, we highlighted the proteins involved in cell wall modification, an enzymatic arsenal that allows the plant pathogens to inhabit environments with oxidative stress, which promotes degradation of plant compounds and facilitates infection. Conclusions: The present work reports six genomes and provides a database of the putative effectorome of Moniliophthora, a first step towards the understanding of the functional basis of fungal pathogenicity. [ABSTRACT FROM AUTHOR]

Published
2018
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29. Changes in Soil Physical and Chemical Properties in Long Lerm Improved Natural and Traditional Agroforestry Management Systems of Cacao Genotypes in Peruvian Amazon.

Author

Arévalo-Gardini, Enrique, Canto, Manuel, Alegre, Julio, Loli, Oscar, Julca, Alberto, and Baligar, Virupax

Subjects
*SOIL physics, *SOIL chemistry, *AGROFORESTRY, *CACAO, *SOIL management, *NATIVE plants
Abstract

Growing cacao (Theobroma cacao L.) in an agroforestry system generates a productive use of the land, preserves the best conditions for physical, chemical and biological properties of tropical soils, and plays an important role in improving cacao production and fertility of degraded tropical soils. The aim of this study was to evaluate the impact of two long term agroforestry systems of cacao management on soil physical and chemical properties in an area originally inhabited by 30 years old native secondary forest (SF). The two agroforestry systems adapted were: improved natural agroforestry system (INAS) where trees without economic value were selectively removed to provide 50% shade and improved traditional agroforestry system (ITAS) where all native trees were cut and burnt in the location. For evaluation of the changes of soil physical and chemical properties with time due to the imposed cacao management systems, plots of 10 cacao genotypes (ICS95, UF613, CCN51, ICT1112, ICT1026, ICT2162, ICT2171, ICT2142, H35, U30) and one plot with a spontaneous hybrid were selected. Soil samples were taken at 0-20, 20-40 and 40-60 cm depths before the installation of the management systems (2004), and then followed at two years intervals. Bulk density, porosity, field capacity and wilting point varied significantly during the years of assessment in the different soil depths and under the systems assessed. Soil pH, CEC, exchangeable Mg and sum of the bases were higher in the INAS than the ITAS. In both systems, SOM, Ext. P, K and Fe, exch. K, Mg and Al+H decreased with years of cultivation; these changes were more evident in the 0-20 cm soil depth. Overall improvement of SOM and soil nutrient status was much higher in the ITAS than INAS. The levels of physical and chemical properties of soil under cacao genotypes showed a marked difference in both systems. [ABSTRACT FROM AUTHOR]

Published
2015
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30. Additional file 4: of Genome sequence and effectorome of Moniliophthora perniciosa and Moniliophthora roreri subpopulations

Author

Ceslaine Barbosa, Fonseca, Rute, Thiago Batista, Barreto, Mariana, Argolo, Caio, Carvalho, Mariana, Amaral, Daniel, Silva, Edson, Arévalo-Gardini, Enrique, Hidalgo, Karina, Franco, Glória, Pirovani, Carlos, Micheli, Fabienne, and Gramacho, Karina

Subjects
3. Good health
Abstract

Figure S1. Venn diagrams: comparison of results of the effectors: (I) Nuclear Location Signal (NLS), (II) small and cysteine rich (SCR), and (III) repeats containing protein (RCP) and effectors predicted by EffectorP. (DOCX 759 kb)

32. Additional file 4: of Genome sequence and effectorome of Moniliophthora perniciosa and Moniliophthora roreri subpopulations

Author

Ceslaine Barbosa, Fonseca, Rute, Thiago Batista, Barreto, Mariana, Argolo, Caio, Carvalho, Mariana, Amaral, Daniel, Silva, Edson, Arévalo-Gardini, Enrique, Hidalgo, Karina, Franco, Glória, Pirovani, Carlos, Micheli, Fabienne, and Gramacho, Karina

Subjects
3. Good health
Abstract

Figure S1. Venn diagrams: comparison of results of the effectors: (I) Nuclear Location Signal (NLS), (II) small and cysteine rich (SCR), and (III) repeats containing protein (RCP) and effectors predicted by EffectorP. (DOCX 759 kb)

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