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2. Skin-interfaced microfluidic biosensors for colorimetric measurements of the concentrations of ketones in sweat.

Author

Yunyun Wu, Xinming Li, Madsen, Kenneth E., Haohui Zhang, Soongwon Cho, Ruihao Song, Nuxoll, Ravi F., Yirui Xiong, Jiaqi Liu, Jingyuan Feng, Tianyu Yang, Kaiqing Zhang, Aranyosi, Alexander J., Wright, Donald E., Ghaffari, Roozbeh, Yonggang Huang, Nuzzo, Ralph G., and Rogers, John A.

Subjects
MICROFLUIDIC devices, DIABETIC acidosis, 3-Hydroxybutyric acid, BLOOD sampling, KETONES, PERSPIRATION
Abstract

Ketones, such as beta-hydroxybutyrate (BHB), are important metabolites that can be used to monitor for conditions such as diabetic ketoacidosis (DKA) and ketosis. Compared to conventional approaches that rely on samples of urine or blood evaluated using laboratory techniques, processes for monitoring of ketones in sweat using on-body sensors offer significant advantages. Here, we report a class of soft, skin-interfaced microfluidic devices that can quantify the concentrations of BHB in sweat based on simple and low-cost colorimetric schemes. These devices combine microfluidic structures and enzymatic colorimetric BHB assays for selective and accurate analysis. Human trials demonstrate the broad applicability of this technology in practical scenarios, and they also establish quantitative correlations between the concentration of BHB in sweat and in blood. The results represent a convenient means for managing DKA and aspects of personal nutrition/wellness. [ABSTRACT FROM AUTHOR]

Published
2024
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3. Soft, skin-interfaced microfluidic systems with integrated immunoassays, fluorometric sensors, and impedance measurement capabilities

Author

Kim, Sungbong, Lee, Boram, Reeder, Jonathan T., Seo, Seon Hee, Lee, Sung-Uk, Hourlier-Fargette, Aurélie, Shin, Joonchul, Sekine, Yurina, Jeong, Hyoyoung, Oh, Yong Suk, Aranyosi, Alexander J., Lee, Stephen P., Model, Jeffrey B., Lee, Geumbee, Seo, Min-Ho, Kwak, Sung Soo, Jo, Seongbin, Park, Gyungmin, Han, Sunghyun, Park, Inkyu, Jung, Hyo-Il, Ghaffari, Roozbeh, Koo, Jahyun, Braun, Paul V., and Rogers, John A.

Published
2020

5. Sweating Rate and Sweat Chloride Concentration of Elite Male Basketball Players Measured With a Wearable Microfluidic Device Versus the Standard Absorbent Patch Method.

Author

Baker, Lindsay B., King, Michelle A., Keyes, David M., Brown, Shyretha D., Engel, Megan D., Seib, Melissa S., Aranyosi, Alexander J., and Ghaffari, Roozbeh

Subjects
PERSPIRATION, WEARABLE technology, BASKETBALL
Abstract

The purpose of this study was to compare a wearable microfluidic device and standard absorbent patch in measuring local sweating rate (LSR) and sweat chloride concentration ([Cl−]) in elite basketball players. Participants were 53 male basketball players (25 ± 3 years, 92.2 ± 10.4 kg) in the National Basketball Association's development league. Players were tested during a moderate-intensity, coach-led practice (98 ± 30 min, 21.0 ± 1.2 °C). From the right ventral forearm, sweat was collected using an absorbent patch (3M Tegaderm™ + Pad). Subsequently, LSR and local sweat [Cl−] were determined via gravimetry and ion chromatography. From the left ventral forearm, LSR and local sweat [Cl−] were measured using a wearable microfluidic device and associated smartphone application-based algorithms. Whole-body sweating rate (WBSR) was determined from pre- to postexercise change in body mass corrected for fluid/food intake (ad libitum), urine loss, and estimated respiratory water and metabolic mass loss. The WBSR values predicted by the algorithms in the smartphone application were also recorded. There were no differences between the absorbent patch and microfluidic patch for LSR (1.25 ± 0.91 mg·cm−2·min−1 vs. 1.14 ±0.78 mg·cm−2·min−1, p =.34) or local sweat [Cl−] (30.6 ± 17.3 mmol/L vs. 29.6 ± 19.4 mmol/L, p =.55). There was no difference between measured and predicted WBSR (0.97 ± 0.41 L/hr vs. 0.89 ± 0.35 L/hr, p =.22; 95% limits of agreement = 0.61 L/hr). The wearable microfluidic device provides similar LSR, local sweat [Cl−], and WBSR results compared with standard field-based methods in elite male basketball players during moderate-intensity practices. [ABSTRACT FROM AUTHOR]

Published
2022
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9. Skin‐Interfaced Microfluidic System with Machine Learning‐Enabled Image Processing of Sweat Biomarkers in Remote Settings

Author

Baker, Lindsay B., primary, Seib, Melissa S., additional, Barnes, Kelly A., additional, Brown, Shyretha D., additional, King, Michelle A., additional, De Chavez, Peter John D., additional, Qu, Shankang, additional, Archer, Julian, additional, Wolfe, Anthony S., additional, Stofan, John R., additional, Carter, James M., additional, Wright, Donald E., additional, Wallace, Jessica, additional, Yang, Da Som, additional, Liu, Shanliangzi, additional, Anderson, John, additional, Fort, Tucker, additional, Li, Weihua, additional, Wright, John A., additional, Lee, Stephen P., additional, Model, Jeffrey B., additional, Rogers, John A., additional, Aranyosi, Alexander J., additional, and Ghaffari, Roozbeh, additional

Published
2022
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13. Soft, skin-interfaced sweat stickers for cystic fibrosis diagnosis and management

Author

Ray, Tyler R., primary, Ivanovic, Maja, additional, Curtis, Paul M., additional, Franklin, Daniel, additional, Guventurk, Kerem, additional, Jeang, William J., additional, Chafetz, Joseph, additional, Gaertner, Hannah, additional, Young, Grace, additional, Rebollo, Steve, additional, Model, Jeffrey B., additional, Lee, Stephen P., additional, Ciraldo, John, additional, Reeder, Jonathan T., additional, Hourlier-Fargette, Aurélie, additional, Bandodkar, Amay J., additional, Choi, Jungil, additional, Aranyosi, Alexander J., additional, Ghaffari, Roozbeh, additional, McColley, Susanna A., additional, Haymond, Shannon, additional, and Rogers, John A., additional

Published
2021
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14. A skin-interfaced microfluidic platform supports dynamic sweat biochemical analysis during human exercise

Author

Cho, Soongwon, Shaban, Samy M., Song, Ruihao, Zhang, Haohui, Yang, Dasom, Kim, Min-Jae, Xiong, Yirui, Li, Xiuyuan, Madsen, Kenneth, Wapnick, Sarena, Zhang, Shifan, Chen, Ziyu, Kim, Jiwon, Guinto, Gianna, Li, Michelle, Lee, Minkyu, Nuxoll, Ravi F., Shajari, Shaghayegh, Wang, Jin, Son, Seongeun, Shin, Jihoon, Aranyosi, Alexander J., Wright, Donald E., Kim, Tae-il, Ghaffari, Roozbeh, Huang, Yonggang, Kim, Dong-Hwan, and Rogers, John A.

Abstract

Blood lactate concentration is an established circulating biomarker for measuring muscle acidity and can be evaluated for monitoring endurance, training routines, or athletic performance. Sweat is an alternative biofluid that may serve similar purposes and offers the advantage of noninvasive collection and continuous monitoring. The relationship between blood lactate and dynamic sweat biochemistry for wearable engineering applications in physiological fitness remains poorly defined. Here, we developed a microfluidic wearable band with an integrated colorimetric timer and biochemical assays that temporally captures sweat and measures pH and lactate concentration. A colorimetric silver nanoplasmonic assay was used to measure the concentration of lactate, and dye-conjugated SiO2nanoparticle–agarose composite materials supported dynamic pH analysis. We evaluated these sweat biomarkers in relation to blood lactate in human participant studies during cycling exercise of varying intensity. Iontophoresis-generated sweat pH from regions of actively working muscles decreased with increasing heart rate during exercise and was negatively correlated with blood lactate concentration. In contrast, sweat pH from nonworking muscles did not correlate with blood lactate concentration. Changes in sweat pH and blood lactate were observed in participants who did not regularly exercise but not in individuals who regularly exercised, suggesting a relationship to physical fitness and supporting further development for noninvasive, biochemical fitness evaluations.

Published
2024
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15. Skin-interfaced microfluidic system with personalized sweating rate and sweat chloride analytics for sports science applications

Author

Baker, Lindsay B., primary, Model, Jeffrey B., additional, Barnes, Kelly A., additional, Anderson, Melissa L., additional, Lee, Stephen P., additional, Lee, Khalil A., additional, Brown, Shyretha D., additional, Reimel, Adam J., additional, Roberts, Timothy J., additional, Nuccio, Ryan P., additional, Bonsignore, Justina L., additional, Ungaro, Corey T., additional, Carter, James M., additional, Li, Weihua, additional, Seib, Melissa S., additional, Reeder, Jonathan T., additional, Aranyosi, Alexander J., additional, Rogers, John A., additional, and Ghaffari, Roozbeh, additional

Published
2020
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16. Skin‐Interfaced Microfluidic Systems that Combine Hard and Soft Materials for Demanding Applications in Sweat Capture and Analysis

Author

Choi, Jungil, primary, Chen, Shulin, additional, Deng, Yujun, additional, Xue, Yeguang, additional, Reeder, Jonathan T., additional, Franklin, Daniel, additional, Oh, Yong Suk, additional, Model, Jeffrey B., additional, Aranyosi, Alexander J., additional, Lee, Stephen P., additional, Ghaffari, Roozbeh, additional, Huang, Yonggang, additional, and Rogers, John A., additional

Published
2020
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17. Soft, skin-interfaced microfluidic systems with integrated enzymatic assays for measuring the concentration of ammonia and ethanol in sweat

Author

Kim, Sung Bong, primary, Koo, Jahyun, additional, Yoon, Jangryeol, additional, Hourlier-Fargette, Aurélie, additional, Lee, Boram, additional, Chen, Shulin, additional, Jo, Seongbin, additional, Choi, Jungil, additional, Oh, Yong Suk, additional, Lee, Geumbee, additional, Won, Sang Min, additional, Aranyosi, Alexander J., additional, Lee, Stephen P., additional, Model, Jeffrey B., additional, Braun, Paul V., additional, Ghaffari, Roozbeh, additional, Park, Chulwhan, additional, and Rogers, John A., additional

Published
2020
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18. Skin-interfaced soft microfluidic systems with modular and reusable electronics for in situ capacitive sensing of sweat loss, rate and conductivity

Author

Hourlier-Fargette, Aurélie, primary, Schon, Stéphanie, additional, Xue, Yeguang, additional, Avila, Raudel, additional, Li, Weihua, additional, Gao, Yiwei, additional, Liu, Claire, additional, Kim, Sung Bong, additional, Raj, Milan S., additional, Fields, Kelsey B., additional, Parsons, Blake V., additional, Lee, KunHyuck, additional, Lee, Jong Yoon, additional, Chung, Ha Uk, additional, Lee, Stephen P., additional, Johnson, Michael, additional, Bandodkar, Amay J., additional, Gutruf, Philipp, additional, Model, Jeffrey B., additional, Aranyosi, Alexander J., additional, Choi, Jungil, additional, Ray, Tyler R., additional, Ghaffari, Roozbeh, additional, Huang, Yonggang, additional, and Rogers, John A., additional

Published
2020
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20. Soft, Skin-Integrated Multifunctional Microfluidic Systems for Accurate Colorimetric Analysis of Sweat Biomarkers and Temperature

Author

Choi, Jungil, primary, Bandodkar, Amay J., additional, Reeder, Jonathan T., additional, Ray, Tyler R., additional, Turnquist, Amelia, additional, Kim, Sung Bong, additional, Nyberg, Nathaniel, additional, Hourlier-Fargette, Aurélie, additional, Model, Jeffrey B., additional, Aranyosi, Alexander J., additional, Xu, Shuai, additional, Ghaffari, Roozbeh, additional, and Rogers, John A., additional

Published
2019
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21. Battery-free, skin-interfaced microfluidic/electronic systems for simultaneous electrochemical, colorimetric, and volumetric analysis of sweat

Author

Bandodkar, Amay J., primary, Gutruf, Philipp, additional, Choi, Jungil, additional, Lee, KunHyuck, additional, Sekine, Yurina, additional, Reeder, Jonathan T., additional, Jeang, William J., additional, Aranyosi, Alexander J., additional, Lee, Stephen P., additional, Model, Jeffrey B., additional, Ghaffari, Roozbeh, additional, Su, Chun-Ju, additional, Leshock, John P., additional, Ray, Tyler, additional, Verrillo, Anthony, additional, Thomas, Kyle, additional, Krishnamurthi, Vaishnavi, additional, Han, Seungyong, additional, Kim, Jeonghyun, additional, Krishnan, Siddharth, additional, Hang, Tao, additional, and Rogers, John A., additional

Published
2019
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23. Soft, skin-interfaced microfluidic systems with integrated immunoassays, fluorometric sensors, and impedance measurement capabilities.

Author

Sungbong Kim, Lee, Boram, Reeder, Jonathan T., Seon Hee Seo, Sung-Uk Lee, Hourlier-Fargette, Aurélie, Joonchul Shin, Yurina Sekine, Hyoyoung Jeong, Yong Suk Oh, Aranyosi, Alexander J., Lee, Stephen P., Model, Jeffrey B., Geumbee Lee, Min-Ho Seo, Sung Soo Kwak, Seongbin Jo, Gyungmin Park, Sunghyun Han, and Inkyu Park

Subjects
GALVANIC skin response, IMMUNOASSAY, PSYCHOLOGICAL stress, PATIENT monitoring, VITAMIN C
Abstract

Soft microfluidic systems that capture, store, and perform biomarker analysis of microliter volumes of sweat, in situ, as it emerges from the surface of the skin, represent an emerging class of wearable technology with powerful capabilities that complement those of traditional biophysical sensing devices. Recent work establishes applications in the real-time characterization of sweat dynamics and sweat chemistry in the context of sports performance and healthcare diagnostics. This paper presents a collection of advances in biochemical sensors and microfluidic designs that support multimodal operation in the monitoring of physiological signatures directly correlated to physical and mental stresses. These wireless, battery-free, skin-interfaced devices combine lateral flow immunoassays for cortisol, fluorometric assays for glucose and ascorbic acid (vitamin C), and digital tracking of skin galvanic responses. Systematic benchtop evaluations and field studies on human subjects highlight the key features of this platform for the continuous, noninvasive monitoring of biochemical and biophysical correlates of the stress state. [ABSTRACT FROM AUTHOR]

Published
2020
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24. Soft Wearable Systems for Colorimetric and Electrochemical Analysis of Biofluids.

Author

Ghaffari, Roozbeh, Choi, Jungil, Raj, Milan S., Chen, Shulin, Lee, Stephen P., Reeder, Jonathan T., Aranyosi, Alexander J., Leech, Adam, Li, Weihua, Schon, Stephanie, Model, Jeffrey B., and Rogers, John A.

Subjects
ELECTROCHEMICAL analysis, COLORIMETRIC analysis, FLEXIBLE electronics, ELECTROCHEMICAL sensors, MICROFLUIDICS, BIOCHEMISTRY
Abstract

Wearable monitoring systems provide valuable insights about the state of wellness, performance, and progression of diseases. Although conventional wearable systems have been effective in measuring a few key biophysical markers, they offer limited insights into biochemical activity and are otherwise cumbersome in ambulatory modes of use, relying on wired connections, mechanical straps, and bulky electronics. Recent advances in skin‐interfaced microfluidics, stretchable/flexible electronics, and mechanics have created new wearable systems with capabilities in real‐time, noninvasive analysis of sweat biochemistry in combination with biophysical metrics. Here, the latest technologies in multifunctional sweat sensing systems are presented with a focus on novel microfluidic designs, fully‐integrated wireless electrochemical sensors, and hybrid biochemical/biophysical sensing capabilities, creating real‐time physiological insights. [ABSTRACT FROM AUTHOR]

Published
2020
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25. Tunable Nanostructured Coating for the Capture and Selective Release of Viable Circulating Tumor Cells

Author

Massachusetts Institute of Technology. Department of Chemical Engineering, Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science, Lim, Eugene J., Li, Wei, Castleberry, Steven, Hammond, Paula T., Aceto, Nicola, Sullivan, James P., Jensen, Anne E., Zeinali, Mahnaz, Martel, Joseph M., Aranyosi, Alexander J., Bardia, Aditya, Sequist, Lecia V., Haber, Daniel A., Maheswaran, Shyamala, Toner, Mehmet, Stott, Shannon L., Reategui, Eduardo, Massachusetts Institute of Technology. Department of Chemical Engineering, Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science, Lim, Eugene J., Li, Wei, Castleberry, Steven, Hammond, Paula T., Aceto, Nicola, Sullivan, James P., Jensen, Anne E., Zeinali, Mahnaz, Martel, Joseph M., Aranyosi, Alexander J., Bardia, Aditya, Sequist, Lecia V., Haber, Daniel A., Maheswaran, Shyamala, Toner, Mehmet, Stott, Shannon L., and Reategui, Eduardo

Abstract

A layer-by-layer gelatin nanocoating is presented for use as a tunable, dual response biomaterial for the capture and release of circulating tumor cells (CTCs) from cancer patient blood. The entire nanocoating can be dissolved from the surface of microfluidic devices through biologically compatible temperature shifts. Alternatively, individual CTCs can be released through locally applied mechanical stress.

Published
2016

26. Tectorial membrane travelling waves underlie abnormal hearing in Tectb mutant mice

Author

Harvard University--MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology. Research Laboratory of Electronics, Ghaffari, Roozbeh, Freeman, Dennis M., Aranyosi, Alexander J., Richardson, Guy P., Harvard University--MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology. Research Laboratory of Electronics, Ghaffari, Roozbeh, Freeman, Dennis M., Aranyosi, Alexander J., and Richardson, Guy P.

Abstract

Remarkable sensitivity and exquisite frequency selectivity are hallmarks of mammalian hearing, but their underlying mechanisms remain unclear. Cochlear insults and hearing disorders that decrease sensitivity also tend to broaden tuning, suggesting that these properties are linked. However, a recently developed mouse model of genetically altered hearing (Tectb[superscript −/−]) shows decreased sensitivity and sharper frequency selectivity. In this paper, we show that the Tectb mutation reduces the spatial extent and propagation velocity of tectorial membrane (TM) travelling waves and that these changes in wave propagation are likely to account for all of the hearing abnormalities associated with the mutation. By reducing the spatial extent of TM waves, the Tectb mutation decreases the spread of excitation and thereby increases frequency selectivity. Furthermore, the change in TM wave velocity reduces the number of hair cells that effectively couple energy to the basilar membrane, which reduces sensitivity. These results highlight the importance of TM waves in hearing., National Institutes of Health (U.S.) (Grant R01-DC000238), National Institutes of Health (U.S.) (Harvard University--MIT Division of Health Sciences and Technology. Speech and Hearing Bioscience and Technology Program. Training Grant)

Published
2015

27. Col11a2 Deletion Reveals the Molecular Basis for Tectorial Membrane Mechanical Anisotropy

Author

Harvard University--MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology. Research Laboratory of Electronics, Ghaffari, Roozbeh, Masaki, Kinuko, Gu, Jianwen Wendy, Chan, Gary, Freeman, Dennis M., Smith, Richard J.H., Aranyosi, Alexander J., Harvard University--MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology. Research Laboratory of Electronics, Ghaffari, Roozbeh, Masaki, Kinuko, Gu, Jianwen Wendy, Chan, Gary, Freeman, Dennis M., Smith, Richard J.H., and Aranyosi, Alexander J.

Abstract

The tectorial membrane (TM) has a significantly larger stiffness in the radial direction than other directions, a prominent mechanical anisotropy that is believed to be critical for the proper functioning of the cochlea. To determine the molecular basis of this anisotropy, we measured material properties of TMs from mice with a targeted deletion of Col11a2, which encodes for collagen XI. In light micrographs, the density of TM radial collagen fibers was lower in Col11a2 –/– mice than wild-types. Tone-evoked distortion product otoacoustic emission and auditory brainstem response measurements in Col11a2 –/– mice were reduced by 30–50 dB independent of frequency as compared with wild-types, showing that the sensitivity loss is cochlear in origin. Stress-strain measurements made using osmotic pressure revealed no significant dependence of TM bulk compressibility on the presence of collagen XI. Charge measurements made by placing the TM as an electrical conduit between two baths revealed no change in the density of charge affixed to the TM matrix in Col11a2 –/– mice. Measurements of mechanical shear impedance revealed a 5.5 ± 0.8 dB decrease in radial shear impedance and a 3.3 ± 0.3 dB decrease in longitudinal shear impedance resulting from the Col11a2 deletion. The ratio of radial to longitudinal shear impedance fell from 1.8 ± 0.7 for TMs from wild-type mice to 1.0 ± 0.1 for those from Col11a2 –/– mice. These results show that the organization of collagen into radial fibrils is responsible for the mechanical anisotropy of the TM. This anisotropy can be attributed to increased mechanical coupling provided by the collagen fibrils. Mechanisms by which changes in TM material properties may contribute to the threshold elevation in Col11a2 –/– mice are discussed., National Institutes of Health (U.S.) (Grant R01-DC00238)

Published
2015

28. Tunable Nanostructured Coating for the Capture and Selective Release of Viable Circulating Tumor Cells

Author

Reátegui, Eduardo, primary, Aceto, Nicola, additional, Lim, Eugene J., additional, Sullivan, James P., additional, Jensen, Anne E., additional, Zeinali, Mahnaz, additional, Martel, Joseph M., additional, Aranyosi, Alexander J., additional, Li, Wei, additional, Castleberry, Steven, additional, Bardia, Aditya, additional, Sequist, Lecia V., additional, Haber, Daniel A., additional, Maheswaran, Shyamala, additional, Hammond, Paula T., additional, Toner, Mehmet, additional, and Stott, Shannon L., additional

Published
2015
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31. Signal Transmission in the Auditory System

Author

Frishkopf, Lawrence S., Kiang, Nelson Y-S., Peake, William T., Siebert, William M., Weiss, Thomas F., Freeman, Dennis M., Delgutte, Bertrand, Eddington, Donald K., Guinan, John J., Jr., Rosowski, John J., Puria, Sunil, Huang, Gregory T., Ravicz, Michael E., Teoh, Su W., Voss, Susan E., Abnet, C. Cameron, Aranyosi, Alexander J., Davis, Charles Quentin, Hattangadi, Shilpa M., Johnson, Laura K., Karu, Zoher Z., Rouf, Rosanne, Shatz, Lisa F., Slifka, Janet L., Cariani, Peter A., Hammond, Benjamin M., Kalluri, Sridhar, McKinney, Martin F., Litovsky, Ruth Y., Dynes, Scott B. C., Shera, Christopher A., Stankovic, Konstantina M., Hall, Robert D., Lighvani, Arash, McKinney, Christopher, Frishkopf, Lawrence S., Kiang, Nelson Y-S., Peake, William T., Siebert, William M., Weiss, Thomas F., Freeman, Dennis M., Delgutte, Bertrand, Eddington, Donald K., Guinan, John J., Jr., Rosowski, John J., Puria, Sunil, Huang, Gregory T., Ravicz, Michael E., Teoh, Su W., Voss, Susan E., Abnet, C. Cameron, Aranyosi, Alexander J., Davis, Charles Quentin, Hattangadi, Shilpa M., Johnson, Laura K., Karu, Zoher Z., Rouf, Rosanne, Shatz, Lisa F., Slifka, Janet L., Cariani, Peter A., Hammond, Benjamin M., Kalluri, Sridhar, McKinney, Martin F., Litovsky, Ruth Y., Dynes, Scott B. C., Shera, Christopher A., Stankovic, Konstantina M., Hall, Robert D., Lighvani, Arash, and McKinney, Christopher

Abstract

Contains table of contents for Section 3, an introduction and reports on seven research projects., National Institutes of Health Grant P01-DC-00119, National Institutes of Health Grant R01-DC-00194, National Institutes of Health Grant R01 DC00238, National Institutes of Health Grant R01-DC02258, National Institutes of Health Grant T32-DC00038, National Institutes of Health Grant P01-DC00361, National Institutes of Health Grant 2RO1 DC00235, National Institutes of Health Contract N01-DC22402

Published
2010

36. Skin-interfaced microfluidic biosensors for colorimetric measurements of the concentrations of ketones in sweat.

Author

Wu Y, Li X, Madsen KE, Zhang H, Cho S, Song R, Nuxoll RF, Xiong Y, Liu J, Feng J, Yang T, Zhang K, Aranyosi AJ, Wright DE, Ghaffari R, Huang Y, Nuzzo RG, and Rogers JA

Subjects
Humans, Ketones urine, Skin chemistry, Skin metabolism, Microfluidic Analytical Techniques instrumentation, Lab-On-A-Chip Devices, Equipment Design, Sweat chemistry, Colorimetry instrumentation, Biosensing Techniques instrumentation, 3-Hydroxybutyric Acid analysis
Abstract

Ketones, such as beta-hydroxybutyrate (BHB), are important metabolites that can be used to monitor for conditions such as diabetic ketoacidosis (DKA) and ketosis. Compared to conventional approaches that rely on samples of urine or blood evaluated using laboratory techniques, processes for monitoring of ketones in sweat using on-body sensors offer significant advantages. Here, we report a class of soft, skin-interfaced microfluidic devices that can quantify the concentrations of BHB in sweat based on simple and low-cost colorimetric schemes. These devices combine microfluidic structures and enzymatic colorimetric BHB assays for selective and accurate analysis. Human trials demonstrate the broad applicability of this technology in practical scenarios, and they also establish quantitative correlations between the concentration of BHB in sweat and in blood. The results represent a convenient means for managing DKA and aspects of personal nutrition/wellness.

Published
2024
Full Text
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