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Parastacus guapo sp. nov. Huber, Araujo & Ribeiro (Figs. 1–6) Type series. Holotype: adult male, Brazil, Rio Grande do Sul, Pantano Grande, Horto Sanga das Pedras, Zona Alagada (30°13’28.4”S, 52°24’44.1”W) 20/X/2020, coll. A.F. Huber; K.M. Gomes & F.B. Ribeiro (MNRJcarcino 030200). Paratype 1: 1 female, same data as holotype (MNRJcarcino 030201); Paratype 3: 1 female, same data as holotype (MNRJcarcino 030202); Paratypes 2, 4–6: 1 male, 3 females and 2 juveniles, same data as holotype (UFRGS 6932). Etymology. Named based on the regional expression “guapo”, originally of spanish language and a typical expression in the state of Rio Grande do Sul, which means beautiful, charming and elegant. It refers to the greenish blue color present principally in chelipeds of this species. We also suggest the common name “guapo crayfish” for this species. Diagnosis. Front with short triangular rostrum. Rostral apex shaped as inverted “U”, with long straight up-ward blunt spine. Eyes small. Suborbital angle>90° and unarmed. Postorbital carinae more prominent in anterior and middle portions. Cervical groove weakly V-shaped. Areola narrow and barely discernible. Telson subrectangular with large sharp spines on lateral margins. Epistome anteromedian lobe pentagonal and anterolateral section with big conical projection ending in a big sharp spine on both sides. Mandible with caudal molar process bicuspidate with one cephalodistal cusp and one medium conical distoproximal cusp; incisive lobe with nine teeth (the third tooth from the anterior margin is the largest). Internal surface of chelipeds palm with sparse tubercules. Description of holotype. Rostrum: triangular, longer than wide (RW 84.6% of RL), short (14.2% of CL), reaching distal portion of second article of antennular peduncle (Fig. 2A–C). Dorsum straight curved downward in anterior portion, apex inverted “U”-shaped, ending in a long straight upward blunt spine (Fig. 2B, C). Few plumose setae on lateral margins. Carinae straight, prominent, narrow, extending back to carapace and long, surpassing rostral basis; rostral carinae sides slightly convergent and rostral carinae basis parallel (Fig. 2B, C). Cephalon: Carapace lacking spines but with some tubercles above the anterior region of the cervical groove (Fig. 2C). CeL 67.1% of CL. Eyes small (CMW 60% of OW); suborbital angle>90°, unarmed (Fig. 2C). Front narrow (FW 45.3% of CW). Postorbital carinae little longer than rostral carinae (RCL 87.1% of POCL) and more prominent in anterior and middle portions, more discreet in final portion. Lateral cephalic edge with moderate setation (Fig. 2A–C). Thorax: carapace laterally compressed, deep and narrow (CD 50.7% of CL; CW 46% of CL). Cervical groove weakly V-shaped. Branchiocardiac grooves inconspicuous (Fig. 2A). Areola narrow, 2.5x as long as wide (AreL 28.5% of CL) (Fig. 2A). Pleon: lacking spines or tubercles, short and wide (PL 71% of CL; PW 82% of CW), smooth, covered with small setae on pleural margins (Fig. 2A). Pleural somites with rounded posterior margins. S1 pleurae with a small distal lobe slightly overlapped by S2 pleurae. S2 pleurae low and short with shallow groove parallel to margin and a deep groove above (Fig. 2E). Tailfan: telson a little less calcified in the distal margin, subrectangular, longer than wide (TeW 84.9% of TeL), with large and sharp spines on lateral margins; rounded distal margin with abundant long plumose setae and short simple setae. Dorsal surface with sparse tufts of short setae and inconspicuous dorsomedian longitudinal groove (Fig. 2A, G). Uropod protopod bilobed, with rounded and unarmed margins and some simple setae in the margin; proximal lobe largest. Exopod lateral margin bears a sharp spine, mid-dorsal carina prominent ending in a sharp spine. Transverse suture (diaeresis) straight with eight dorsolateral spines (outer) and six dorsolateral spines (inner) on both exopods. Endopod, mid-dorsal carina prominent, ending in a big sharp spine; lateral margin with one sharp spine at level of exopod transverse suture (Fig. 2G). Epistome: anterolateral section with big conical projection ending in a big sharp spine on both sides, with three and four small tubercles between the apex and basis of the conical projection in the left and right sides respectively, and a small circular median concavity. Posterolateral section smooth and with lateral grooves converging to the basis of the anteromedian lobe. Median section with a longitudinal groove deeper close to the anteromedian lobe. Anteromedian lobe pentagonal, apex rounded by use, a little wider than long, with some sparse serrated setae, reaching median part of first article of antennal peduncle; dorsal surface concave with a groove from the middle of the lateral sides to the apex and basis with a shallow groove (Fig. 3A). Thoracic sternites: SLP4 small and close to each other, median keel present and not inflated; SLP5 same size of SPL4 and close to each other, median keel present and not inflated; SLP6 larger than SLP4, SLP5 and similar size to SLP8 with a slightly concave surface, median keel present and not inflated; SLP7 largest and with surface slightly concave, median keel inflated, bullar lobes absent; SLP8 median size with and slightly concave, median keel absent, vertical arms of paired sternopleural bridges separated to each other, bullar lobes absent (Fig. 3B, C). Antennule: internal ventral border of basal article without spines (Fig. 3A). Antenna: when extended back reaching the posterior margin of SIII.Antennal scale widest at midlength, reaching the middle of the third antennal article, ASW 43.3% of ASL (Fig. 3A, D), lateral margin straight and distal spine sharp and well developed. Coxa with weakly prominent carina above nephropore and three blunt spines different in size laterally distributed. Basis unarmed (Fig. 3A). Mandible: cephalic molar process molariform, caudal molar process bicuspidate with one bigger cephalodistal cusp and one medium conical distoproximal cusp. Incisive lobe with nine teeth. The third tooth from the anterior margin is the largest (Fig. 3E). Third maxilliped: ischium with some tubercles with tufts of short and composed setae on the middle portion and tufts of long simple setae on inner margin of ventral surface (Fig. 3F, G); dorsal surface with some sparse simple setae close to the outer margin (Fig. 3G); crista dentata bearing 24 teeth in the right and 21 in the left ischium (Fig. 3F, G). Merum, dorsal surface glabrous. Merum ventral surface sparsely covered by long smooth simple setae in the median region and with serrated setae on the distal of inner margin (Fig. 3H); Exopod longer than ischium, with flagellum reaching the initial ⅓ of merum and with tufts of long and composed setae in the last articles and tufts of composed setae in the basal article (Fig. 3F, G). First pair of pereiopods (chelipeds): large and subequal, laterally flattened (RPrT 24.5% of RPrL; LPrT 24.7% of LPrL) (Fig. 2A; Fig. 3H, I). Ischium ventral surface with 28 tubercles on both sides. Merus: right merus (RML) 56.7% of propodus length (RPrL); left merus (LML) 56.9% of propodus length (LPrL); ventral surface with two longitudinal series of tubercles: inner series with 21 tubercles, outer 24, plus 53 mesial tubercles irregularly distributed on right merus; inner series with 21 tubercles, outer 24, plus 55 mesial tubercles irregularly distributed on left merus; dorsal and midventral spines medium sizes and blunts. Carpus with dorsomedial surface with a shallow longitudinally groove and the distal margin with short and simple setae (Fig. 2A; Fig. 3H, I). Internal dorsolateral margin with row of tubercles, increasing in size distally; inner surface with 37 and 33 small mesial tubercles in right and left sizes respectively. Carpal spine present and small. Propodus moderate (RPrW and LPrW) 43.5% of length in right cheliped and 42.6% in left cheliped. Dorsal surface of palm with 2–3 rows of verrucous tubercles irregularly distributed (Fig. 3H, I). Inner margin with sparse tubercles more concentrated in the low portion and close to the insertion of dactylus. Ventral surface bearing 2-3 irregularly rows of squamose tubercles, surpassing the beginning of the fixed finger (Fig. 3H). Dactylus: moving subvertically, right dactylus (RDL) 63.1% of propodus length (RPrL), left dactylus (LDL) 60.3% of left propodus (LPrL); dorsal surface with squamose tubercles decreasing in size from the basis to the apex of dactylus (Fig. 3I). Cutting edge of fingers visible. Fixed finger with thirteen teeth, sixth the largest, and twelve teeth, fourth the largest, on right and left sides respectively. Dactylus with sixteen and fourteen teeth in the right and left sides respectively, with the third teeth largest on both sides (Fig. 3H, I). Second pair of pereiopods: dorsal surface of dactylus and ventral surface of propodus with tufts of short and long simple setae, dorsal surface of propodus with long and simple setae. Dorsal surface of carpus with long composed setae (Fig. 3J). Gonopores: Presence of both genital apertures on coxae of third and fifth pairs of pereiopods. Female gonopores semi-ellipsoidal (maximum diameter 1.43 mm) with well-calcified membrane. Male gonopores rounded, opening onto the apical end of a small, fixed, calcified and truncated phallic papilla, close to the inner border of the ventral surface of coxae of the fifth pair of pereiopods. Male cuticle partition present (Fig. 3B). Branchial count: 20 + epr + r. Branchial arrangement follows the same described by Huxley (1879) and Hobbs (1991) with the epipod of the first maxilliped with rudimentary podobranchial filaments. Measurements. Holotype male, CL 31.06 mm and TL 64.47 mm. In type series, CL ranging from 19.85 to 31.06 mm (23.1 ± 3.8 mm). FW/CW: 0.45 ± 0.02 (min: 0.42; max: 0.47). RW/RL: 0.81 ± 0.07 (min: 0.70; max: 0.90). CMW/OW: 0.73 ± 0.07 (min: 0.59; max: 0.81). Postorbital carina longer or equal to rostral carina in all specimens analyzed. CW/PW: 1.11 ± 0.08 (min: 1.01; max: 1.22). AreW/RW: 0.89 ± 0.07 (min: 0.76; max: 0.96) (Table 1). Color of living specimens. Rostrum greenish blue to greenish-brown. Cephalothorax anterior and lateral regions brown with shades of light brown to greenish-blue. First pair of pereiopods greenish-blue on the external surface, greenish-blue with shades of light brown in internal surface and apex of dactylus and propodus orange-red to reddish-brown. Pereiopod pairs 2–5 light brown to light greenish-blue in the dorsal and lateral regions and light brown in the ventral region. Dorsal pleon and taifan brown with shades of light greenish-brown, low portions of lateral margins of pleon with shades of greenish-blue (Fig. 4). Variations in paratypes. All paratypes present both masculine and feminine gonopores in the same individual. Male paratypes also present female gonopores semi-ellipsoidal (average maximum diameter 1.2 mm) covered by a calcified membrane. Male gonopores are very similar in all male paratypes. Front wide in paratype 1 (FW / CW = 47.5%). Rostrum of paratypes 3 and 5 long once they reach the distal portion of the third article of antennule. Suborbital angle 90° in paratype 5. Rostral and postorbital carena of similar sizes in paratypes 2 and 3. Pleon long in paratypes 2 and 5 (PL / CL = 82.9% and 80.3%, respectively). S2 pleura moderate in paratypes 1 and 4, long in paratype 6, high and moderate in paratype 3 and high and long in paratype 5. Number of spines in exopod of tailfan varies between eight or nine dorsolateral spines (outer) and four, six or seven in dorsolateral spines (inner) on right side and eight or nine dorsolateral spines (outer) and five or six dorsolateral spines (inner) on the left side. Epistome anteromedian lobe longer than wide in paratype 2. Antennule internal ventral border of the basal article with one spine in the middle portion of left side in paratype 1 and with spines on both sides in paratypes 2, 4, 5 and 6. Antenna when extended back reaching the posterior margin of fourth pleura in paratypes 2 and 6. Antennal scale reaches the proximal margin of the third antennal article of antennule in paratype 1. Incisive lobe of the mandible has eight teeth in paratypes 6. The number of teeth in the crista dentata ranges from 21—27 in the left ischium and from 20—32 in the right ischium of the third maxilliped in the paratypes. Merus midventral spine doubles in paratype 5. Carpal spine more prominent and sharp in paratypes 1, 3 and 4. Dorsal spine sharp in both merus of chelipeds in paratypes 2 and 3. The number of teeth in fixed fingers of chelipeds and dactylus have a wide range of variation in size and number, which is probably related to the smaller size of paratypes when compared to holotype or regenerated chelipeds. Remarks. Parastacus guapo sp. nov. is morphologically similar to P. brasiliensis, P. buckupi, P. macanudo and P. promatensis in the general shape of rostrum, rostral carina length, shape of telson (subrectangular) and epistome anteromedian lobe pentagonal (Fig. 2A–C, G; 3A; Table 2). However, it differs from: P. brasiliensis in rostral surface straight, sub-orbital angle> 90°, postorbital carinae prominent in anterior and middle portions, carpal spine present, internal surface of chelipeds palm with sparse tubercules, areola narrow and pleon short (Fig. 2A–C; 3A, H, I; Table 2); P. buckupi in the same characters that P. brasiliensis, except the areola narrow, and is also different for mandible incisive lobe with nine teeth (the third tooth from the anterior margin is the largest), mandible caudal molar process bicuspidate, internal surface of chelipeds palm with sparse tubercules and telson with large lateral spines (Fig. 2A–C, G; 3E, H, J; Table 2); P. macanudo in a bigger rostral spine, postorbital carinae prominent in anterior and middle portions, mandible incisive lobe with the third tooth the largest, 2–3 rows of verrucose tubercles irregularly distributed in the palm dorsal surface of chelipeds, internal surface of chelipeds palm with sparse tubercules and the habitat (Fig. 2A–C; 3E, H, J; Table 2); P. promatensis in the apex of rostrum rounded, sub-orbital angle> 90°, postorbital carinae prominent in anterior and middle portions, cervical groove “V” shaped, carpal spine present, 2–3 rows of verrucose tubercles irregularly distributed in the palm dorsal surface of chelipeds and pleon short; however both species have internal surface of chelipeds palm with sparse tubercules (Fig. 2 A-D; 3H; Table 2). Parastacus guapo sp. nov. differs from all other Parastacus species in having the epistome anterolateral section with large conical projection ending in a large sharp spine on both sides (Fig. 3A). Phylogenetic position. The phylogenetic analysis suggests, with high values of reliability (bootstrap and posterior probability), that P. guapo sp. nov. is a distinct species of P. brasiliensis s.s., Lineages A–E, and other species of the genus included (Fig. 5). The genetic distances estimated between P. guapo sp. nov. and P. brasiliensis s. s. was 5.35% (Sup. Table. 2). Besides that, the lineages range from 3.41% (Lineage C) to 5.95% (Lineage E), and of other species from 4.05% (P. buckupi) to 5.90% (P. tuerkayi) (Sup. Table. 2). Habitat and ecology. Parastacus guapo sp. nov. was collected in a wetland area, near a small stream, inside the Sanga das Pedras project of CMPC (Fig. 1). This forestry area is located in the Baixo Jacuí hydrographic basin in the central region of the state of Rio Grande do Sul (Fig. 6). The area is part of the physiographic region of Depressão Central, almost in the division with the Planalto Sul Rio Grandense region, and of the Pampa biome (IBGE 2004a; 2004b; SIGBio-RS 2021). ......continued on the next page Vegetation. The region has as predominant vegetation grassy-woody steppe with gallery forests or riparian vegetation associated with streams and their overflow zones (Fig 6 A, B). In addition to the presence of forestry areas of Eucalyptus sp., the native plant formations of the region are mainly composed of grasses and bushes of the families Poaceae (Paspalum spp. L., Andropogon lateralis Nees —“Capim Caninha’’ and Mnesithea selloana (Hack.) de Koning & Sosef), Asteraceae (Baccharis ochracea Spreng. —“Vassoura do Campo”, Vernonanthura nudiflora (Less.) H.Rob., Senecio selloi (Spreng.) DC and Senecio brasiliensis (Spreng.) Less. —“Maria Mole”), Cyperaceae (Eleocharis spp. R.Br., Rhynchosia diversifolia Micheli. and Cyperus spp. L.), Fabaceae (Desmodium incanum (Sw.) DC —“Pega Pega”, Trifolium polymorphum Poir. —“Trevo” and Macroptilium prostratum (Benth.) Urb.), Solanaceae (Solanum spp. L.) and some small trees like Myrtaceae (Psidium cattleianum Sabine —“Araçá”) (Caumo et al. 2020) (Fig. 6). Soil. The soil is classified as Dystrophic Red Argisols, but mixed with Dystrophic Red-Yellow Argisols (Santos et al. 2011). These types of soils have accentuated red colors due to higher levels of iron oxides and usually occur in areas of undulating relief, but they can be identified in less steep areas. They have low fertility that could be explained by relief slope, nutrient limitations, strong acidity, high drainage capacity and also high susceptibility to erosion and degradation (AGEITEC 2021a; Santos et al. 2011; 2021a; 2021b; Streck et al. 2002; 2008). Burrowing behavior and burrow structure. According to observations of burrows structure and characteristics of the habitat, Parastacus guapo sp. nov. can be classified as a primary burrower with types 1a, b burrows, due to the construction of complex tunnels close and with direct and indirect connections to permanent water bodies (Hobbs 1942; Horwitz & Richardson 1986; Richardson, 2007) (Fig. 6). This species builds burrow galleries of 1–4 interconnected tunnels, which can reach a depth up to 1m ending in a life chamber. The entries of the galleries are delimited by simple holes or by chimneys, which can reach up to 8 cm high and wide (Fig. 6C, D). Distribution. Parastacus guapo sp. nov. appears to have limited distribution, being registered only in the municipality of Pantano Grande, central region of Rio Grande do Sul, southern Brazil (Fig. 1). Conservation status. Data Deficient. The EOO and AOO were estimated as approximately 937 km ² and 1, 87 km ² (or 0, 38 km ² considering only preserved habitat), respectively (Fig. 1). According to the sub-criteria B1 and B2, an EOO

Uptaded key to Parastacus The following key is modified from proposed in Rogers et al. (2020) with the addition of Parastacus macanudo, P. gomesae sp. nov. and P. guapo sp. nov.. The modifications of the original key and new steps start in step number eight (8) and these were elaborated according to information from Ribeiro et al. (2016; 2017; 2020) and Huber et al. (2018; 2020). 1 Telson with lateral spines............................................................................... 2 1’ Telson without lateral spines, maxilliped III exopod flagellum surpassing ischium distal margin............................................................................................... Parastacus nicoleti (Philippi, 1882) 2(1) Postorbital carina proximal edge ending in a strong spine; uropod protopod proximal lobe bearing a spine; areola delimited by carinae.............................................................................................. 3 2’ Postorbital carina proximal edge not ending in spine; uropod protopod lobe lacking spine; areola not delimited by carinae.. 4 3(2) Cheliped merus, carpus, and propodus medially with tufts of long setae; maxilliped III ischium ventral margin with longitudinal spine row................................................................ Parastacus varicosus Faxon, 1898 3’ Cheliped merus, carpus, and propodus medially glabrous; maxilliped III ischium ventral margin with longitudinal tubercle row...................................................................... Parastacus saffordi Faxon, 1898 4(2’) Telson distal margin rounded........................................................................... 5 4’ Telson distal margin acute.................................... Parastacus buckupi Huber, Ribeiro & Araujo, 2018 5(4) Cheliped carpus distally glabrous; epistome anteromedian lobe hexagonal or pentagonal............................ 6 5’ Cheliped carpus distally with tufts of long and simples setae; epistome anteromedial lobe heptagonal........................................................................... Parastacus pilicarpus Huber, Ribeiro & Araujo, 2018 6(5) Cheliped dactyl and pollex cutting edges obscured by long setal tufts............................................ 7 6’ Cheliped dactyl and pollex cutting edges visible............................................................. 8 7(6) Postorbital carinae prominent.......................................... Parastacus pilimanus (von Martens, 1869) 7’ Postorbital carinae obsolete....................................... Parastacus laevigatus Buckup and Rossi, 1980 8(6) Chelipeds with globose palm............................................................................ 9 8’ Chelipeds with flattened palm.......................................................................... 11 9(8) Cervical groove U-shaped.................... Parastacus caeruleodactylus Ribeiro and Araujo in Ribeiro et al., 2016 9’ Cervical groove V-shaped.............................................................................. 10 10(9’) Rostral carinae reaching postorbital carinae proximal edge.......................... Parastacus defossus Faxon, 1898 10’ Rostral carinae surpassing postorbital carinae proximal edge...................... Parastacus pugnax (Poeppig, 1835) 11(8’) Telson subrectangular................................................................................ 12 11’ Telson subtriangular.............................. Parastacus fluviatilis Ribeiro and Buckup in Ribeiro et al., 2016 12(11) Cheliped palm dorsal surface with tubercles irregularly distributed or with irregular tubercle rows.................... 13 12’ Cheliped palm dorsal surface with three well-defined tubercle rows....................................................................................... Parastacus tuerkayi Ribeiro, Huber and Araujo in Ribeiro et al., 2017 13(12) Cheliped ventral surface bearing one row of squamose tubercles............................................... 16 13’ Cheliped ventral surface bearing two rows of squamose tubercles.............................................. 14 14(13’) Telson lateral margins with large spines................................................................... 15 14’ Telson lateral margins with small spines............................................ Parastacus gomesae sp. nov. 15(14) Cephalon postorbital carinae prominent in anterior and middle portions..................... Parastacus guapo sp. nov. 15’ Cephalon postorbital carinae weakly prominent....... Parastacus macanudo Huber, Rockhill, Araujo and Ribeiro, 2020 16(13) Cheliped palm medial margin with medial area tuberculate, groove U-shaped.................................................................................................. Parastacus promatensis Fontoura & Conter, 2008 16’ Cheliped palm medial margin smooth; groove weakly V-shaped............ Parastacus brasiliensis (von Martens, 1869), Published as part of Huber, Augusto Frederico, Araujo, Paula Beatriz & Ribeiro, Felipe Bezerra, 2022, The hole is deeper: description of two new species within the Parastacus brasiliensis (von Martens, 1869) species complex with an integrative taxonomy approach, pp. 251-284 in Zootaxa 5168 (3) on pages 275-276, DOI: 10.11646/zootaxa.5168.3.1, http://zenodo.org/record/6882776, {"references":["Rogers, D. C., Magalhaes, C., Peralta, M., Ribeiro, F. B., Bond-Buckup, G., Price, W. W., Guerrero-Kommritz, J., Mantelatto, F. L., Bueno, A., Camacho, A. I., Gonzalez, E. R., Jara, C. G., Pedraza, M., Pedraza-Lara, C., Latorre, E. R. & Santos, S. (2020) Chapter 23 - Phylum Arthropoda: Crustacea: Malacostraca. In: Rogers, D., Christopher; Damborenea, C. & Thorp, J. (Org.), Thorp and Covich's Freshwater Invertebrates. Vol. 5. 4 th Edition. Academic Press, Boston, Massachusetts, pp. 809 - 986. [ISBN 9780128042250] https: // doi. org / 10.1016 / B 978 - 0 - 12 - 804225 - 0.00023 - X.","Ribeiro, F. B., Buckup, L., Gomes, K. M. & Araujo, P. B. (2016) Two new species of South American freshwater crayfish genus Parastacus Huxley, 1879 (Crustacea: Decapoda: Parastacidae). Zootaxa, 4158 (3), 301 - 324. https: // doi. org / 10.11646 / zootaxa. 5158.3.1","Huber, A. F., Ribeiro, F. B. & Araujo, P. B. (2018) New endemic species of freshwater crayfish Parastacus Huxley, 1879 (Crustacea: Decapoda: Parastacidae) from the Atlantic forest in southern Brazil. Nauplius, 26, e 2018015. https: // doi. org / 10.1590 / 2358 - 2936 e 2018015","Philippi, R. A. (1882) Zoologia chilena. Sobre los Astacus. Anales de la Universidad de Chile, 61, 624 - 628. https: // doi. org / 10.5354 / 0717 - 8883.2012.21621","Faxon, W. (1898) Observations on the Astacidae in the United States National Museum and in the Museum of Comparative Zoology, with descriptions of new species. Proceedings of the United States National Museum, 20 (1136), 642 - 694. https: // doi. org / 10.5479 / si. 00963801.20 - 1136.643","von Martens, E. (1869) Sudbrasilische Suss- und Brackwasser-Crustaceen nach den Sammlungen des Dr. Reinh. Hensel. Archiv fur Naturgeschichte, 35, 1 - 37.","Buckup, L. & Rossi, A. (1980) O Genero Parastacus no Brasil (Crustacea, Decapoda, Parastacidade). Revista Brasieira de Biologia, 40 (4), 663 - 681.","Huber, A. F., Rockhill, E. R., Araujo, P. B. & Ribeiro, F. B. (2020) A new species of burrowing crayfish in genus Parastacus Huxley, 1879 (Decapoda, Parastacidae) from the Sinos River Basin, Southern Brazil. Zoological Studies, 59 (47). https: // doi. org / 10.6620 / ZS. 2020.59 - 47","Fontoura, N. F. & Conter, M. R. (2008) Description of a new subspecies of the crayfish Parastacus brasiliensis (Von Martens, 1869) from Sao Francisco de Paula, RS, Brazil (Decapoda, Parastacidae). Zootaxa, 1849 (1), 28 - 34. https: // doi. org / 10.11646 / zootaxa. 1849.1.2"]}

In this contribution, we describe two new species of burrowing crayfish species complex Parastacus brasiliensis from forestry areas in the central region of the state of Rio Grande do Sul, southern Brazil. We used an integrative taxonomy approach with morphology and the mitochondrial DNA 16S rRNA gene and also evaluated their conservation status according to the IUCN Red List Criteria. Parastacus guapo sp. nov. was collected near the municipality of Pantano Grande and it differs from all other congeneric species in having an epistome anterolateral section with a large conical projection ending in a big sharp spine on both sides. It also differs from P. brasiliensis sensu stricto in the shape of the rostral surface, sub-orbital angle, postorbital carina straight, sub-orbital angle > 90°, postorbital carinae prominent in anterior and middle portions, carpal spine present, the internal surface of chelipeds palm with sparse tubercules, areola narrow and pleon short. Parastacus gomesae sp. nov. was collected in the municipality of São Jerônimo, it differs from all analysed species for rostral spine absent, epistome anteromedian lobe heptagonal and mandible incisive process with seven teeth (the second tooth from the anterior margin is the largest). It is also distinguished of P. brasiliensis sensu stricto in the sub-orbital angle > 90°, postorbital carinae prominent in anterior and middle portions, 3–4 rows of verrucose tubercles irregularly distributed on the palm dorsal surface of chelipeds, areola narrow and pleon short. Phylogenetic relationships confirmed the distinct position of these new species to the already described species. The extent of occurrence (EOO) / area of occurrence (AOO) of P. guapo sp. nov and P. gomesae sp. nov. were estimated at 937 km² / 1,87 km², and 2.107 km² / 23,9 km² respectively. The main threats identified were continued decline in the quality of habitats, resulting from deforestation and forestry areas. However, as we know only one point of occurrence for each new species, we suggest that both be categorized as Data Deficient.

Cymothoa excisa Perty, 1833 (Figs. 1, 6; Table 1) Restricted synonymy: Cymothoa excisa Perty,1833: 211, tab. 40, fig. 11. Coelho & Koenig, 1972: 1976, table 1. Trilles 1994: 141. Thatcher et al., 2003: 545, figs. 27-51. Luque et al., 2013: 1454. Material examined: BRAZIL: Bahia: one ovigerous female, Candeias, coll. J. Pezzi da Silva, 11/V/2010 (MCP 2996); two ovigerous females, Candeias, coll. J. Pezzi da Silva, 11/V/2010 (MCP 2997) [illustrated]. Remarks: Cymothoa excisa was most recently diagnosed by Thatcher et al. (2003). The species had already been recorded from northeastern Brazil, in the states of Maranhão, Pernambuco and Sergipe by Coelho & Koenig (1972). These authors also raised the possibility of the occurrence in the states of Piauí, Ceará, Rio Grande do Norte, Paraíba, Alagoas, Bahia and Espírito Santo, the latter in the southeastern Brazil. In addition, the occurrence of this species was also recorded in the states of Pará (Monod,1969) and Rio de Janeiro (Richardson,1901).This is the first record for the state of Bahia, particularly in the northern portion (12°44′49″S 38°29′38″W). The southernmost record of C. excisa in western South Atlantic is La Plata (Argentina) (Gerstaecker, 1901). Cymothoa excisa was redescribed by Thatcher et al. (2003) based on material from the Florianopolis Island, state of Santa Catarina, southern Brazil. Females are distinguished mainly by the acute anterolateral angles of pereonite 1 reaching or surpassing the eyes; the truncate cephalon, deeply immersed in pereonite 1; eyes present but inconspicuous; pereonites 4-6 subequal in width; pleon deeply immersed in pereonite 7 (Fig. 1); and pleopods trilaminate (Thatcher et al., 2003). Males are similar to females, but smaller and with pleopod 2 lacking an appendix masculinum (Thatcher et al., 2003). Hosts: Thehostofpresentspecimensisunknown.However, C. excisa was already found parasitizing fishes of the families Gerreidae, Haemulidae, Lutjanidae, Priacanthidae, Sciaenidae and Synodontidae (Joca et al., 2015). Distribution: USA (Massachusetts to Florida), Antilles, Venezuela, Brazil (Pará, Maranhão, Pernambuco, Bahia, Sergipe, Rio de Janeiro and Santa Catarina)and Argentina (Trilles, 1994; Thatcher et al., 2003) (Fig. 6)., Published as part of Ribeiro, Felipe Bezerra, Huber, Augusto Frederico & Araujo, Paula Beatriz, 2021, Redescription of the fish-parasitic isopod Cymothoa ianuarii Schioedte & Meinert, 1884 and further records of C. excisa Perty, 1833 and C. oestrum (Linnaeus, 1758) (Isopoda: Cymothoida: Cymothoidae) from Brazil, pp. 1-10 in Papéis Avulsos de Zoologia 61 on page 2, DOI: 10.11606/1807-0205/2021.61.09, http://zenodo.org/record/5007563, {"references":["Perty, M. 1833. Delectus animalium articulatorum, quae in itinere per Brasiliam annis 1817 - 1820, jussu et auspiciis Maximiliani Josephi I. Bavariae regis augustissimi peracto, collegerunt Dr. J. B. Spix et Dr. C. F. Ph. de Martius. Monachii, Imprensis Editoris.","Coelho, P. A. & Koenig, M. L. 1972. A distribuicao dos crustaceos pertencentes as ordens Stomatopoda, Tanaidacea e Isopoda no Norte e Nordeste de Brasil. Trabalhos do Instituto Oceanografico da Universidade Federal de Pernambuco, Recife, 13: 245 - 260.","Trilles, J. P. 1994. Catalogue mondial des Cymothoidae. Prodrome pour une faune. Studia Marina, 21 / 22 (1 - 2): 5 - 288.","Thatcher, V. E.; Loyola e Silva, J.; Jost, G. F. & Souza-Conceicao, J. M. 2003. Comparative morphology of Cymothoa spp. (Isopoda, Cymothoidae) from Brazilian fishes, with the description of Cymothoa catarinensis sp. nov. and redescriptions of C. excisa Perty and C. oestrum (Linnaeus). Revista Brasileira de Zoologia, 20 (3): 541 - 552.","Luque, J. L.; Vieira, F. M.; Takemoto, R. M.; Pavanelli, G. C. & Eiras, J. V. 2013. Checklist of Crustacea parasitizing fishes from Brazil. Check List, 9 (6): 1449 - 1470.","Monod, T. 1969. Sur trois crustaces isopodes marins de la region Guyane- Amazone. Cahiers ORSTOM, Serie Oceanographie, 7 (3): 47 - 68.","Richardson, H. 1901. Key to the Isopods of the Atlantic coast of North America, with description of new and little-known species. Proceedings of the United States Natural History Museum, 23 (1222): 493 - 579.","Gerstaecker, A. 1901. Isopoda. In: Bronn, H. C. G. (Ed.). Die Klassen und Ordnungen der Arthropoden wissenschaftlich dargestellt in Wort und Bild., Funfter Band. II Abtheilung. Crustacea. Isopoda. Leipzig, C. F. Winter'sche verlagshadlung. p. 8 - 278.","Joca, L. K.; Leray, V. L.; Zigler, K. S. & Brusca, R. C. 2015. A new host and reproduction at a small size for the \" snapper-choking isopod \" Cymothoa excisa (Isopoda: Cymothoidae). Journal of Crustacean Biology, 35 (2): 292 - 294."]}

Iansaoniscus iraquara Campos-Filhoı Araujo and Taitiı 2017 Material examined Bahia: 13ı Itaet��ı Lapa do Bode caveı 31 July 2010ı leg. ME Bichuetteı B Rantin and JE Gall��o (LES 6358); 13ı Iraquaraı Gruta Natal caveı 4 August 2004ı leg. ME Bichuette and R Bessi (LES 6364); 13ı Iraquaraı Buraco do C��o caveı 20 June 2009ı leg. ME Bichuette and L Senna-Horta (LES 6363). Distribution Iansaoniscus iraquara was originally described from Buraco do C��o caveı Iraquaraı state of Bahia (Campos-Filho et al. 2017c). Our new records extended its distribution for Gruta Natal and Lapa do Bode cavesı in Iraquara and Itaet��ı respectively., Published as part of Camile Sorbo Fernandes, Ivanklin Soares Campos-Filho, Paula Beatriz Araujo & Bichuette, Maria Elina, 2019, Synopsis of terrestrial isopods (Crustacea: Isopoda: Oniscidea) from Brazilian caves�� with emphasis on new records from north�� midwest�� northeast and southeast regions, pp. 1095-1129 in Journal of Natural History 53 (17) on page 1118, DOI: 10.1080/00222933.2019.1634225, http://zenodo.org/record/3673375, {"references":["Campos-Filho ISi Montesanto Gi Araujo PBi Taiti S. 2017 c. New species and new records of terrestrial isopods (Crustaceai Isopodai Oniscidea) from Brazil. Iheringiai Serie Zool. 107: e 2017034. doi: 10.1590 / 1678 - 4766 e 2017034"]}

Spelunconiscus castroi Campos-Filhoı Araujo and Taitiı 2014 Distribution Gruta MOC-32 caveı Matozinhosı state of Minas Gerais (Campos-Filho et al. 2014 ı 2018a)., Published as part of Camile Sorbo Fernandes, Ivanklin Soares Campos-Filho, Paula Beatriz Araujo & Bichuette, Maria Elina, 2019, Synopsis of terrestrial isopods (Crustacea: Isopoda: Oniscidea) from Brazilian caves�� with emphasis on new records from north�� midwest�� northeast and southeast regions, pp. 1095-1129 in Journal of Natural History 53 (17) on page 1104, DOI: 10.1080/00222933.2019.1634225, http://zenodo.org/record/3673375, {"references":["Campos-Filho ISi Araujo PBi Bichuette MEi Trajano Ei Taiti S. 2014. Terrestrial isopods (Crustacea: Isopoda: Oniscidea) from Brazilian caves. Zool J Linn Soc. 172: 360 - 425. doi: 10.1111 / zoj. 2014.172. issue- 2.","Campos-Filho ISi Cardoso GMi Aguiar JO. 2018 a. Catalogue of terrestrial isopods (Crustaceai Isopodai Oniscidea) from Brazil: an update with some considerations. Nauplius. 26: e 2018038. doi: 10.1590 / 2358 - 2936 e 2018038."]}

Circoniscus buckupi Campos-Filho and Araujoı 2011 Distribution Several caves in FLONA Caraj��s (FLONA ��� National Forest)ı Parauapebası state of Par�� (see localities on Campos-Filho and Araujo 2011)., Published as part of Camile Sorbo Fernandes, Ivanklin Soares Campos-Filho, Paula Beatriz Araujo & Bichuette, Maria Elina, 2019, Synopsis of terrestrial isopods (Crustacea: Isopoda: Oniscidea) from Brazilian caves�� with emphasis on new records from north�� midwest�� northeast and southeast regions, pp. 1095-1129 in Journal of Natural History 53 (17) on page 1110, DOI: 10.1080/00222933.2019.1634225, http://zenodo.org/record/3673375

Metaprosekia caupe Campos-Filhoı Araujo and Taitiı 2014 Material examined Par��ı Altamira: Sugiro-Roncador caveı 2 April 2011ı leg. ME Bichuetteı DM Schimonskyı JE Gall��o and D Pedroso (LES: 13ı 2♀ ı 6316; 1♀ ı 6317; 13ı 6318; 2♀ ı 6319; 2♀ ı 6320; 13ı 6323); Abrigo Igarap�� caveı 14 December 2010ı leg. ME Bichuetteı DM Schimonsky and JE Gall��o (LES: 13ı 6302; 3♀ ı 6303); 6♀ ı Abrigo Paratizinho caveı 4 April 2011ı leg. ME Bichuetteı DM Schimonskyı JE Gall��o and D Pedroso (LES 6324); Abrigo Cama de Vara caveı 2 April 2011ı leg. ME Bichuetteı DM Schimonskyı JE Gall��o and D Pedroso (LES: 5♀ ı 6325; 6♀ ı 6326); 13ı Abrigo Turi�� caveı 5 April 2011ı leg. ME Bichuetteı DM Schimonskyı JE Gall��o and D Pedroso (LES 6322). Distribution Several caves at Altamiraı state of Par��. Remarks Metaprosekia caupe was originally described from Caverna Sugiro-Roncador caveı Altamiraı state of Par�� (Campos-Filho et al. 2014). The present records considerably expand its distribution for several caves in Altamira region. It is regarded as troglophile., Published as part of Camile Sorbo Fernandes, Ivanklin Soares Campos-Filho, Paula Beatriz Araujo & Bichuette, Maria Elina, 2019, Synopsis of terrestrial isopods (Crustacea: Isopoda: Oniscidea) from Brazilian caves�� with emphasis on new records from north�� midwest�� northeast and southeast regions, pp. 1095-1129 in Journal of Natural History 53 (17) on pages 1107-1108, DOI: 10.1080/00222933.2019.1634225, http://zenodo.org/record/3673375, {"references":["Campos-Filho ISi Araujo PBi Bichuette MEi Trajano Ei Taiti S. 2014. Terrestrial isopods (Crustacea: Isopoda: Oniscidea) from Brazilian caves. Zool J Linn Soc. 172: 360 - 425. doi: 10.1111 / zoj. 2014.172. issue- 2."]}

Trichorhina pataxosi Campos-Filhoı Bichuette and Taitiı 2016 Material examined Minas Geraisı Matozinhos: August-September 2016ı leg. Equipe Spelayon: 13ı Caverna BM 050 caveı August-September 2016 (LES 10950); 1 juvenileı same locality (LES 10952); 1♀ ı 2 juvenilesı Caverna BM 104 cave (LES 10961); 5♀ ı 3 juvenisı 1 mancaı same locality (LES 11031); 2♀ ı Caverna BM 013 cave (LES 10976); 13ı 1♀ ı Caverna BM 024 cave (LES 10980); 13ı 1♀ ı same locality (LES 10981); 1♀ ı Caverna BM 064 cave (LES 11007); 1♀ ı 2 mancası Caverna BM 085 cave (LES 11021); 1♀ ı Caverna BM 111 cave (LES 11035); 1♀ ı Caverna BM 142 cave (LES 11050); 1♀ ı Caverna BM 152 cave (LES 11051)ı 1♀ ı Caverna BM 157 cave (LES 11052); 13ı 2 juvenilesı same locality (LES 11053); 1♀ ı Caverna SUPRAM 003 caveı same date and collectors (LES 11054). Distribution Trichorhina pataxosi occurs at two caves from Pedro Leopoldo and at the caves from Matozinhosı state of Minas Gerais. Remarks New record. Troglophilic species. Trichorhina pataxosi was described from Gruta do Sufoco and Gruta do Nei cavesı state of Minas Gerais (Campos-Filho et al. 2016). The caves mentioned here are inserted in the same karst regionı Bambu�� Groupı ca. 20 km from the type locality (see Campos-Filho et al. 2016)., Published as part of Camile Sorbo Fernandes, Ivanklin Soares Campos-Filho, Paula Beatriz Araujo & Bichuette, Maria Elina, 2019, Synopsis of terrestrial isopods (Crustacea: Isopoda: Oniscidea) from Brazilian caves�� with emphasis on new records from north�� midwest�� northeast and southeast regions, pp. 1095-1129 in Journal of Natural History 53 (17) on page 1115, DOI: 10.1080/00222933.2019.1634225, http://zenodo.org/record/3673375, {"references":["Lopez-Orozco CMi Carpio-Diaz YMi GRN Si Campos-Filho IS. 2016. A new species and first record of Androdeloscia (Oniscidea: Philosciidae) from Colombia. Stud Neotrop Fauna E. 52 (1): 1 - 7."]}

Novamundoniscus sp. 3 Material examined Bahia: 2♀ ı Carinhanhaı Caverna Bem Bom caveı 6 December 2012ı leg. ME Bichuette and JE Gall��o (LES 6440). Goi��sı S��o Domingos: 1♀ ı Lapa do Ang��lica caveı 18 June 2012ı leg. DM Schimonskyı JE Gall��o and LB Sim��es (LES 6395); Terra Ronca I caveı 30 October 2012ı leg. ME Bichuetteı JE Gall��oı LB Sim��esı CS Fernandes and T Zepon (LES: 13ı 1♀ ı 6409; 1♀ ı 6412); 13ı Terra Ronca II caveı 6 October 2011ı leg. ME Bichuette and LB Sim��es (LES 6386); 1♀ ı near S��o Domingos River (Epigean)ı 25 April 2012ı leg. DM Schimonskyı JE Gall��o and CS Fernandes (LES 6370); 1♀ ı Russ��o System (Epigean)ı 23 April 2011ı leg. ME Bichuetteı PP Rizzato and JE Gall��o (LES 6381). Distribution Novamundoniscus sp. 3 was found in- and outside of the caves listed above. Remarks New record. Non-troglomorphic. The specimens examined show body pigments light brownı eyes constituted of ten ommatidia and elongated antennae and uropod exopod. Considering these characters and its occurrence in- and outside cavesı this species is considered troglophilic., Published as part of Camile Sorbo Fernandes, Ivanklin Soares Campos-Filho, Paula Beatriz Araujo & Bichuette, Maria Elina, 2019, Synopsis of terrestrial isopods (Crustacea: Isopoda: Oniscidea) from Brazilian caves�� with emphasis on new records from north�� midwest�� northeast and southeast regions, pp. 1095-1129 in Journal of Natural History 53 (17) on page 1113, DOI: 10.1080/00222933.2019.1634225, http://zenodo.org/record/3673375

Benthana taeniata Araujo and Buckupı 1994 Distribution Widely distributed species in many Brazilian states (see distribution on Campos-Filho et al. 2015b). Benthana taeniata was also recorded inside the Gruta Zeferino I caveı S��o Roque de Minası Serra da Canastraı state of Minas Gerais (Campos-Filho et al. 2014)., Published as part of Camile Sorbo Fernandes, Ivanklin Soares Campos-Filho, Paula Beatriz Araujo & Bichuette, Maria Elina, 2019, Synopsis of terrestrial isopods (Crustacea: Isopoda: Oniscidea) from Brazilian caves�� with emphasis on new records from north�� midwest�� northeast and southeast regions, pp. 1095-1129 in Journal of Natural History 53 (17) on page 1106, DOI: 10.1080/00222933.2019.1634225, http://zenodo.org/record/3673375, {"references":["Campos-Filho ISi Taiti Si Araujo PB. 2015 b. Taxonomic revision of the genus Benthana Budde-Lundi 1908 (Isopoda: Oniscidea: Philosciidae). Zootaxa. 4022: 1 - 73. doi: 10.11646 / zootaxa. 4022.1.1.","Campos-Filho ISi Araujo PBi Bichuette MEi Trajano Ei Taiti S. 2014. Terrestrial isopods (Crustacea: Isopoda: Oniscidea) from Brazilian caves. Zool J Linn Soc. 172: 360 - 425. doi: 10.1111 / zoj. 2014.172. issue- 2."]}

Trichorhina curupira Campos-Filhoı Araujo and Taitiı 2014 Material examined Par��ı Altamira: Caverna Pedra da Cachoeira caveı 15 December 2010ı leg. ME Bichuetteı DM Schimonsky and JE Gall��o (LES: 103ı 11♀ ı 6304; 13ı 5♀ ı 6308); same localityı 3 April 2011ı leg. ME Bichuetteı DM Schimonskyı JE Gall��o and D Pedroso (LES: 2♀ ı 6314; 103ı 14♀ ı 6327; 13ı 5♀ ı 6329; 63ı 34♀ ı 6330; 73ı 6♀ ı 6331). Distribution Caverna Pedra da Cachoeira caveı Altamiraı state of Par�� (Campos-Filho et al. 2014)., Published as part of Camile Sorbo Fernandes, Ivanklin Soares Campos-Filho, Paula Beatriz Araujo & Bichuette, Maria Elina, 2019, Synopsis of terrestrial isopods (Crustacea: Isopoda: Oniscidea) from Brazilian caves�� with emphasis on new records from north�� midwest�� northeast and southeast regions, pp. 1095-1129 in Journal of Natural History 53 (17) on page 1114, DOI: 10.1080/00222933.2019.1634225, http://zenodo.org/record/3673375, {"references":["Campos-Filho ISi Araujo PBi Bichuette MEi Trajano Ei Taiti S. 2014. Terrestrial isopods (Crustacea: Isopoda: Oniscidea) from Brazilian caves. Zool J Linn Soc. 172: 360 - 425. doi: 10.1111 / zoj. 2014.172. issue- 2."]}

Novamundoniscus altamiraensis Campos-Filhoı Araujo and Taitiı 2014 Distribution Abrigos Assurini caveı Altamiraı state of Par�� (Campos-Filho et al. 2014)., Published as part of Camile Sorbo Fernandes, Ivanklin Soares Campos-Filho, Paula Beatriz Araujo & Bichuette, Maria Elina, 2019, Synopsis of terrestrial isopods (Crustacea: Isopoda: Oniscidea) from Brazilian caves�� with emphasis on new records from north�� midwest�� northeast and southeast regions, pp. 1095-1129 in Journal of Natural History 53 (17) on page 1112, DOI: 10.1080/00222933.2019.1634225, http://zenodo.org/record/3673375, {"references":["Campos-Filho ISi Araujo PBi Bichuette MEi Trajano Ei Taiti S. 2014. Terrestrial isopods (Crustacea: Isopoda: Oniscidea) from Brazilian caves. Zool J Linn Soc. 172: 360 - 425. doi: 10.1111 / zoj. 2014.172. issue- 2."]}

Trichorhina sp. 3 Material examined Goi��sı S��o Domingos: 13ı Lapa do Ang��lica caveı 18 June 2012ı leg. DM Schimonskyı JE Gall��o and LB Sim��es (LES 6394); 4♀ ı Gruta dos Revolucion��rios caveı 20 June 2012ı leg. DM Schimonskyı JE Gall��o and LB Sim��es (LES 6405); 1♀ ı Caverna Terra Ronca II caveı 1 October 2012ı leg. ME Bichuetteı JE Gall��oı LB Sim��esı CS Fernandes and T Zepon (LES 6408). Distribution Lapa do Ang��licaı Gruta dos Revolucion��rios and Terra Ronca II caveı S��o Domingosı state of Goi��s. Remarks New record. The specimens examined show eyes reduced and body pigments absentı which could be related with endogean lifestyle (Souza et al. 2011; Campos-Filho et al. 2014)., Published as part of Camile Sorbo Fernandes, Ivanklin Soares Campos-Filho, Paula Beatriz Araujo & Bichuette, Maria Elina, 2019, Synopsis of terrestrial isopods (Crustacea: Isopoda: Oniscidea) from Brazilian caves�� with emphasis on new records from north�� midwest�� northeast and southeast regions, pp. 1095-1129 in Journal of Natural History 53 (17) on pages 1116-1117, DOI: 10.1080/00222933.2019.1634225, http://zenodo.org/record/3673375, {"references":["Souza LAi Araujo JPi Campos-Filho IS. 2011. The genus Trichorhina Budde-Lund in Brazili with description of seven new species (Isopodai Oniscideai Platyarthridae). Iheringia Zool. 101: 239 - 261. doi: 10.1590 / S 0073 - 47212011000200012.","Campos-Filho ISi Araujo PBi Bichuette MEi Trajano Ei Taiti S. 2014. Terrestrial isopods (Crustacea: Isopoda: Oniscidea) from Brazilian caves. Zool J Linn Soc. 172: 360 - 425. doi: 10.1111 / zoj. 2014.172. issue- 2."]}

Xangoniscus odara Campos-Filhoı Bichuette and Taitiı 2016 Distribution Lapa do Cip�� caveı Itacarambiı state of Minas Gerais (Campos-Filho et al. 2016)., Published as part of Camile Sorbo Fernandes, Ivanklin Soares Campos-Filho, Paula Beatriz Araujo & Bichuette, Maria Elina, 2019, Synopsis of terrestrial isopods (Crustacea: Isopoda: Oniscidea) from Brazilian caves�� with emphasis on new records from north�� midwest�� northeast and southeast regions, pp. 1095-1129 in Journal of Natural History 53 (17) on page 1105, DOI: 10.1080/00222933.2019.1634225, http://zenodo.org/record/3673375, {"references":["Lopez-Orozco CMi Carpio-Diaz YMi GRN Si Campos-Filho IS. 2016. A new species and first record of Androdeloscia (Oniscidea: Philosciidae) from Colombia. Stud Neotrop Fauna E. 52 (1): 1 - 7."]}

Armadillidae gen. et sp. 1 Material examined Bahiaı Carinhanha: 1♀ ı Gruna do Valdecir caveı 31 May 2012ı leg. ME Bichuetteı JE Gall��o and N Hattori (LES 6432). Distribution Gruna do Valdecir caveı Carinhanhaı state of Bahia. Remarks New record. The specimen examined shows body weakly pigmented and eyes composed of 12 ommatidiaı indicating that the species probably is troglomorphic. As in other lineages of Oniscideaı the lack of males difficult species level identification. Future surveys will help in the correct species recognition., Published as part of Camile Sorbo Fernandes, Ivanklin Soares Campos-Filho, Paula Beatriz Araujo & Bichuette, Maria Elina, 2019, Synopsis of terrestrial isopods (Crustacea: Isopoda: Oniscidea) from Brazilian caves�� with emphasis on new records from north�� midwest�� northeast and southeast regions, pp. 1095-1129 in Journal of Natural History 53 (17) on page 1121, DOI: 10.1080/00222933.2019.1634225, http://zenodo.org/record/3673375

Neotroponiscus tuberculatus Cardoso and Araujoı 2017 Distribution Iron ore caves from Brumadinhoı state of Minas Gerais. Family PUDEONISCDAE Lemos de Castroı 1973, Published as part of Camile Sorbo Fernandes, Ivanklin Soares Campos-Filho, Paula Beatriz Araujo & Bichuette, Maria Elina, 2019, Synopsis of terrestrial isopods (Crustacea: Isopoda: Oniscidea) from Brazilian caves�� with emphasis on new records from north�� midwest�� northeast and southeast regions, pp. 1095-1129 in Journal of Natural History 53 (17) on page 1118, DOI: 10.1080/00222933.2019.1634225, http://zenodo.org/record/3673375

Iuiuniscus iuiuensis Souzaı Ferreira and Sennaı 2015 Material examined Bahiaı Iuiu: 33ı 2♀ ı Lapa do Baix��o caveı Serra do Ramalho (LES 14353). Distribution Lapa do Baix��o caveı Iuiuı state of Bahia (Souza et al. 2015)., Published as part of Camile Sorbo Fernandes, Ivanklin Soares Campos-Filho, Paula Beatriz Araujo & Bichuette, Maria Elina, 2019, Synopsis of terrestrial isopods (Crustacea: Isopoda: Oniscidea) from Brazilian caves�� with emphasis on new records from north�� midwest�� northeast and southeast regions, pp. 1095-1129 in Journal of Natural History 53 (17) on page 1104, DOI: 10.1080/00222933.2019.1634225, http://zenodo.org/record/3673375, {"references":["Souza LAi Ferreira RLi Senna AR. 2015. Amphibious shelter-builder Oniscidea species from the New World with description of a new subfamilyi a new genus and a new species from Brazilian Cave (Isopodai Synochetai Styloniscidae). PLoS One. 10 (5): e 0115021. doi: 10.1371 / journal. pone. 0115021."]}

Trichorhina anhanguera Campos-Filhoı Araujo and Taitiı 2014 Distribution Gruta MP-10 caveı Morro do Piları state of Minas Gerais (Campos-Filho et al. 2014)., Published as part of Camile Sorbo Fernandes, Ivanklin Soares Campos-Filho, Paula Beatriz Araujo & Bichuette, Maria Elina, 2019, Synopsis of terrestrial isopods (Crustacea: Isopoda: Oniscidea) from Brazilian caves�� with emphasis on new records from north�� midwest�� northeast and southeast regions, pp. 1095-1129 in Journal of Natural History 53 (17) on page 1114, DOI: 10.1080/00222933.2019.1634225, http://zenodo.org/record/3673375, {"references":["Campos-Filho ISi Araujo PBi Bichuette MEi Trajano Ei Taiti S. 2014. Terrestrial isopods (Crustacea: Isopoda: Oniscidea) from Brazilian caves. Zool J Linn Soc. 172: 360 - 425. doi: 10.1111 / zoj. 2014.172. issue- 2."]}

Trichorhina sp. 4 Material examined Bahiaı S��o Desid��rio: Gruta Lapa do Manga I caveı 5 November 2008ı leg. ME Bichuetteı T Scatolini and D Pedroso (LES: 13ı 2♀ ı 6273; 23ı 3♀ ı 6274); Buraco da Sopradeira caveı 23 April 2012ı leg. DM Schimonskyı JE Gall��o and CS Fernandes (LES: 13ı 3♀ ı 6289; 13ı 6290); 1♀ ı Gruta Baixa Fria caveı 4 November 2008ı leg. ME Bichuetteı T Scatolini and D Pedroso (LES 6272); 1 juvenileı Gruta do Juraci caveı 3 November 2011ı leg. ME Bichuetteı JE Gall��oı CS Fernandes and D Pedroso (LES 6283); 1♀ ı Gruta do Cat��o cave (Epigean)ı 3 November 2012ı leg. DM Schimonskyı JE Gall��o and CS Fernandes (LES 6296). Distribution Gruta Lapa do Manga Iı Buraco da Sopradeiraı Gruta Baixa Friaı Gruta do Juraci and Gruta do Cat��o cavesı all in S��o Desid��rioı state of Goi��s. Remarks New record. The specimens examined lack eyes and body pigments. They were collected in- and outside cavesı supporting its troglophilic status., Published as part of Camile Sorbo Fernandes, Ivanklin Soares Campos-Filho, Paula Beatriz Araujo & Bichuette, Maria Elina, 2019, Synopsis of terrestrial isopods (Crustacea: Isopoda: Oniscidea) from Brazilian caves�� with emphasis on new records from north�� midwest�� northeast and southeast regions, pp. 1095-1129 in Journal of Natural History 53 (17) on page 1117, DOI: 10.1080/00222933.2019.1634225, http://zenodo.org/record/3673375

Trichorhina yiara Campos-Filhoı Araujo and Taitiı 2014 Distribution Abrigo do Sism��grafo and Abrigo do Abutre cavesı Altamiraı state of Par�� (Campos- Filho et al. 2014)., Published as part of Camile Sorbo Fernandes, Ivanklin Soares Campos-Filho, Paula Beatriz Araujo & Bichuette, Maria Elina, 2019, Synopsis of terrestrial isopods (Crustacea: Isopoda: Oniscidea) from Brazilian caves�� with emphasis on new records from north�� midwest�� northeast and southeast regions, pp. 1095-1129 in Journal of Natural History 53 (17) on page 1115, DOI: 10.1080/00222933.2019.1634225, http://zenodo.org/record/3673375

Gabunillo aridicola Souzaı Senna and Kuryı 2010 Distribution Gruta do Sobradinho caveı Aiuabaı state of Cear��ı and Lajedo da Soledadeı Apodiı state of Rio Grande do Norte (Souza et al. 2010). Remarks Souza et al. (2010) described the second species of the genus Gabunillo, G. aridicolla Souzaı Senna and Kuryı 2010 ı with specimens collected in the Brazilian states of Cear�� and Rio Grande do Norte. The species was included in the genus Gabunillo based on the shape of cephalonı pereonites 2 and 3 without ventral lobesı telson trianguları shape of uropods and pleopod exopods without pleopodal lungs (see also Schmalfuss and Ferrara 1983). The authors mentioned that the uropod of G. aridicola resembles the genera synarmadillo Dollfusı 1892ı Ethelumoris Richardsonı 1907 and Togarmadillo Schmalfuss and Ferraraı 1983 (see also Ferrara and Schmalfuss 1976; Schmalfuss and Ferrara 1983). Howeverı comparing the description of G. coecus and mentioned genera with G. aridicola ı it is possible to observe some differences: the uropod protopod with concave depression bearing glandular pores and distal margin rounded (vs. absent and indented in G. coecus; present in species of synarmadillo)ı and uropod exopod is inserted on median portion of the protopod (vs. exopod inserted near distal margin in G. coecus and all genera). Moreoverı on SEM photographs of the pleopod exopods of G. aridicola ı it seems to have covered monospiracular pleopodal lungs (see Figure 3C and E in Souza et al. 2010). Future morphological analyses are needed to elucidate if the pleopod exopods of G. aridicola have pleopodal lungs and confirm its taxonomic statement. Thus farı G. aridicola differs from all mentioned taxa in having the cephalon with frontal shield fused with vertex (vs. frontal shield well marked in all mentioned generaı except G. coecus)ı posterior corner of the pereonite 1 epimera with short schisma (vs. schisma along all epimera in Ethelumoris and synarmadillo; half of its pereonite length in Togarmadillo)ı and pereonite 2 and 3 epimera wihout ventral lobe (vs. ventral lobe in Ethelumoris and synarmadillo) (see Ferrara and Taiti 1976; Schmalfuss and Ferrara 1983; Souza et al. 2010). Thusı in the light of the above considerationsı we recommend a re-examination of the type-material to confirm or not the placement of the species in the genus Gabunillo., Published as part of Camile Sorbo Fernandes, Ivanklin Soares Campos-Filho, Paula Beatriz Araujo & Bichuette, Maria Elina, 2019, Synopsis of terrestrial isopods (Crustacea: Isopoda: Oniscidea) from Brazilian caves�� with emphasis on new records from north�� midwest�� northeast and southeast regions, pp. 1095-1129 in Journal of Natural History 53 (17) on page 1119, DOI: 10.1080/00222933.2019.1634225, http://zenodo.org/record/3673375, {"references":["Souza LAi Senna ARi Kury AB. 2010. A new species and first record of Gabunillo Schmalfuss and Ferrarai 1983 (Isopodai Oniscideai Armadillidae) from the Neotropics. Zootaxa. 2677: 1 - 14. doi: 10.11646 / zootaxa. 2677.1.1.","Schmalfuss Hi Ferrara F. 1983. Terrestrial isopods from West Africai Part 3: family Armadillidae Verhoeffi 1917. Monit Zool Iti Nuova Seriei Supplemento. 18: 111 - 157. doi: 10.1080 / 00269786.1983. 11758568","Ferrara Fi Schmalfuss H. 1976. Terrestrial isopods from West Africa. Part 1: family \" Eubelidae \". Monitore zool. ital. (N. S.) Suppl. 7: 1 - 114. doi: 10.1080 / 03749444.1976.10736823","Ferrara Fi Taiti S. 1976. Description of a new terrestrial isopod (Crustacea: Oniscoidea) from central Africa: Kivudillo benoiti n. gen. n. sp. Monitore zool. Ital. (N. S.) Suppl. 8: 1 i 203 - 211. doi: 10.1080 / 03749444.1976. 10736836"]}

Xangoniscus itacarambiensis Bastos-Pereiraı Souza and Ferreiraı 2017 Distribution Olhos D ��� ��gua Caveı Northern Minas Geraisı Brazil (Bastos-Pereira et al. 2017)., Published as part of Camile Sorbo Fernandes, Ivanklin Soares Campos-Filho, Paula Beatriz Araujo & Bichuette, Maria Elina, 2019, Synopsis of terrestrial isopods (Crustacea: Isopoda: Oniscidea) from Brazilian caves�� with emphasis on new records from north�� midwest�� northeast and southeast regions, pp. 1095-1129 in Journal of Natural History 53 (17) on page 1105, DOI: 10.1080/00222933.2019.1634225, http://zenodo.org/record/3673375, {"references":["Bastos-Pereira Ri Souza LAi Ferreira RL. 2017. A new amphibious troglobitic styloniscid from Brazil (Isopodai Oniscideai Synocheta). Zootaxa. 4294 (2): 292 - 300. doi: 10.11646 / zootaxa. 4294.2."]}

Trichorhina tomentosa (Budde-Lundı 1893) Material examined S��o Pauloı Eldorado: 13♀ ı Caverna do Diabo cave (Gruta da Tapagem)ı Parque Estadual Caverna do Diaboı 24��38 ��� 17 ��� S 48��24 ��� 04 ��� Wı 16 ��� 20 April 2009ı leg. Pellegati-Franco et al. (LES 3056). Distribution Pantropical species (Schmalfuss 2003). Remarks Accidental species in caves. This is the first record of T. tomentosa in the subterranean environment., Published as part of Camile Sorbo Fernandes, Ivanklin Soares Campos-Filho, Paula Beatriz Araujo & Bichuette, Maria Elina, 2019, Synopsis of terrestrial isopods (Crustacea: Isopoda: Oniscidea) from Brazilian caves�� with emphasis on new records from north�� midwest�� northeast and southeast regions, pp. 1095-1129 in Journal of Natural History 53 (17) on page 1115, DOI: 10.1080/00222933.2019.1634225, http://zenodo.org/record/3673375, {"references":["Schmalfuss H. 2003. World catalog of terrestrial isopods (Isopoda: Oniscidea). Stuttgart Beit Nat. 654: 1 - 341."]}

Cylindroniscus sp. Material examined Goi��sı S��o Domingos: 13ı Lapa do Ang��lica caveı 5 October 2011ı leg. ME Bichuette and LB Sim��es (LES 6388); 1♀ ı Lapa do Ang��lica caveı 18 June 2012ı leg. DM Schimonskyı JE Gall��o and LB Sim��es (LES 6396). Distribution Lapa do Ang��lica caveı S��o Domingosı state of Bahia. Remarks New record. The specimens examined show the body slenderı and absence of eyes and body pigments. As in the previous speciesı these characters can be related with endogean way of life. Further surveys in- and outside caves are necessary to confirm its subterranean classification., Published as part of Camile Sorbo Fernandes, Ivanklin Soares Campos-Filho, Paula Beatriz Araujo & Bichuette, Maria Elina, 2019, Synopsis of terrestrial isopods (Crustacea: Isopoda: Oniscidea) from Brazilian caves�� with emphasis on new records from north�� midwest�� northeast and southeast regions, pp. 1095-1129 in Journal of Natural History 53 (17) on pages 1103-1104, DOI: 10.1080/00222933.2019.1634225, http://zenodo.org/record/3673375

Neotroponiscus iporangaensis Cardoso and Araujoı 2017 Distribution s everal limestone caves from Parque Estadual Tur��stico do Alto Ribeira (PETAR)ı Iporangaı state of S��o Paulo (Cardoso and Araujo 2017)., Published as part of Camile Sorbo Fernandes, Ivanklin Soares Campos-Filho, Paula Beatriz Araujo & Bichuette, Maria Elina, 2019, Synopsis of terrestrial isopods (Crustacea: Isopoda: Oniscidea) from Brazilian caves�� with emphasis on new records from north�� midwest�� northeast and southeast regions, pp. 1095-1129 in Journal of Natural History 53 (17) on page 1118, DOI: 10.1080/00222933.2019.1634225, http://zenodo.org/record/3673375, {"references":["Cardoso GMi Araujo PBi Bichuette ME. 2017. Two new species of Neotroponiscus Arcangelii 1936 (Crustaceai Isopodai Oniscidea) from Brazilian caves. Stud Neotrop Fauna E. 52 (2): 122 - 130. doi: 10.1080 / 01650521.2017.1299440."]}

Trichorhina cipoensis Campos-Filhoı Bichuette and Taitiı 2016 Distribution Lapa do Cip�� caveı state of Minas Gerais (Campos-Filho et al. 2016)., Published as part of Camile Sorbo Fernandes, Ivanklin Soares Campos-Filho, Paula Beatriz Araujo & Bichuette, Maria Elina, 2019, Synopsis of terrestrial isopods (Crustacea: Isopoda: Oniscidea) from Brazilian caves�� with emphasis on new records from north�� midwest�� northeast and southeast regions, pp. 1095-1129 in Journal of Natural History 53 (17) on page 1114, DOI: 10.1080/00222933.2019.1634225, http://zenodo.org/record/3673375, {"references":["Lopez-Orozco CMi Carpio-Diaz YMi GRN Si Campos-Filho IS. 2016. A new species and first record of Androdeloscia (Oniscidea: Philosciidae) from Colombia. Stud Neotrop Fauna E. 52 (1): 1 - 7."]}

Novamundoniscus sp. 1 Material examined Bahiaı S��o Desid��rio: Gruta do Cat��o caveı 30 October 2011ı leg. ME Bichuetteı JE Gall��oı CS Fernandes and D Pedroso (LES: 2♀ ı 6275; 2♀ ı 6276); same locality as previousı 20 March 2012ı leg. DM Schimonskyı JE Gall��o and CS Fernandes (LES: 1♀ ı 6284; 4♀ ı 6285; 1♀ ı 6286); same locality as previousı 3 November 2012ı ME Bichuetteı JE Gall��oı LB Sim��esı CS Fernandes and T Zepon (LES: 1♀ ı 6293; 13ı 1♀ ı 6294; 1♀ ı 6295); Gruta Sumidouro do Jo��o Baio caveı 2 November 2011ı ME Bichuetteı J. Gall��oı CS Fernandes and D Pedroso (LES: 2♀ ı 6277; 1♀ ı 6278; 2♀ ı 6279); 1♀ ı Gruta 159 caveı 7 November 2008ı leg. ME Bichuetteı T Scatolini and D Pedroso (LES 6268); Gruta Sucupira caveı 31 October 2011ı leg. ME Bichuetteı JE Gall��oı CS Fernandes and D Pedroso (LES: 1♀ ı 6280; 1♀ ı 6281; 13ı 6282); 1♀ ı Po��o de Pedra caveı 21 April 2012ı leg. DM Schimonskyı JE Gall��o and CS Fernandes (LES 6291). Distribution Novamundoniscus sp. 1 was recorded only at S��o Desid��rioı state of Bahia. Remarks New record. Non-troglomorphic. The specimens were collected in- and outside cavesı supporting its troglophilic status., Published as part of Camile Sorbo Fernandes, Ivanklin Soares Campos-Filho, Paula Beatriz Araujo & Bichuette, Maria Elina, 2019, Synopsis of terrestrial isopods (Crustacea: Isopoda: Oniscidea) from Brazilian caves�� with emphasis on new records from north�� midwest�� northeast and southeast regions, pp. 1095-1129 in Journal of Natural History 53 (17) on pages 1112-1113, DOI: 10.1080/00222933.2019.1634225, http://zenodo.org/record/3673375

Cubaris murina Brandtı 1833 Material examined Bahiaı S��o Desid��rio: 13ı 2♀ ı Gruta Lapa do Manga IIı 5 November 2008ı leg. ME Bichuetteı T Scatolini and D Pedroso (LES 6271); 1♀ ı Morro do Chap��uı Gruta dos Brej��es caveı 15 May 2009ı leg. ME Bichuette and L Senna-Horta (LES 6360); 13 same locality as previousı 31 December 2012ı leg. L Senna-Horta (LES 6361). Distribution Circumtropical species (Schmalfuss 2003). In Brazilian cavesı it was first recorded at Gruta dos Brej��es caveı Morro do Chap��u (Campos-Filho et al. 2014); our new record extends its distribution to Gruta Lapa do Manga II caveı S��o Desid��rioı Bahia., Published as part of Camile Sorbo Fernandes, Ivanklin Soares Campos-Filho, Paula Beatriz Araujo & Bichuette, Maria Elina, 2019, Synopsis of terrestrial isopods (Crustacea: Isopoda: Oniscidea) from Brazilian caves�� with emphasis on new records from north�� midwest�� northeast and southeast regions, pp. 1095-1129 in Journal of Natural History 53 (17) on page 1119, DOI: 10.1080/00222933.2019.1634225, http://zenodo.org/record/3673375, {"references":["Schmalfuss H. 2003. World catalog of terrestrial isopods (Isopoda: Oniscidea). Stuttgart Beit Nat. 654: 1 - 341.","Campos-Filho ISi Araujo PBi Bichuette MEi Trajano Ei Taiti S. 2014. Terrestrial isopods (Crustacea: Isopoda: Oniscidea) from Brazilian caves. Zool J Linn Soc. 172: 360 - 425. doi: 10.1111 / zoj. 2014.172. issue- 2."]}

Circoniscus bezzii Arcangeliı 1931 Distribution Circoniscus bezzii is recorded from the Brazilian states of Esp��rito Santoı Mato Grossoı Minas Gerais and S��o Pauloı and with dubious record from Paraguay (Souza-Kury and Lemos de Castro 1991; Schultz 1995; Schmidt 2007). In cavesı it is recorded from Caverna Vereda da Palha caveı Presidente Oleg��rioı state of Minas Gerais (Campos-Filho et al. 2014). Remarks Arcangeli (1931) described Circoniscus bezzii from Carandasinhoı state of Mato Grosso. Souza-Kury and Lemos de Castro (1991) extended its distribution for the states of Esp��rito Santoı Minas Gerais and S��o Paulo. Schultz (1995) recorded this species from province of Canendiyuı Paraguayı mentioned that C. gracilidens Souza and Lemos de Castroı 1991 [synonym of C. incisus (see Schmidt 2007)]ı C. incisus Souza and Lemos de Castroı 1991 ı and C. pallidus Arcangeliı 1936 probably are synonyms of C. bezzii (the author did not include these species in the synonym list of C. bezzii)ı and misinterpreted its distribution for the Brazilian state of Rio de Janeiro to Rio de La Plata drainage; the latter without accuracy (this drainage is located between Argentina and Uruguay) (see Schultz 1995). Leistikow and W��gele (1999) considered the paper of Schultz (1995) and misunderstood the distribution of C. bezzii for the states of P��ra and Minas Gerais. This species has never been recorded from Par�� and this misinterpretation was followed by subsequent authors (Schmalfuss 2003; Campos-Filho et al. 2014 ı 2018a). Schmidt (2007) with material from Rio de Janeiro and S��o Paulo re-described the species and based on pereonites 1 and 2 epimera with ventral lobesı mentioned that Schultz ��� s specimens probably belong to C. incisus. Regarding the previous informationı the correct distribution of C. bezzii are those localities in Souza- Kury and Lemos de Castro (1991) ı Schmidt (2007) and Campos-Filho et al. (2014). This species occurs in Atlantic forest and Pantanal areas (Souza-Kury and Lemos de Castro 1991; Schmidt 2007)ı and it is considered accidental in cave environments., Published as part of Camile Sorbo Fernandes, Ivanklin Soares Campos-Filho, Paula Beatriz Araujo & Bichuette, Maria Elina, 2019, Synopsis of terrestrial isopods (Crustacea: Isopoda: Oniscidea) from Brazilian caves�� with emphasis on new records from north�� midwest�� northeast and southeast regions, pp. 1095-1129 in Journal of Natural History 53 (17) on page 1109, DOI: 10.1080/00222933.2019.1634225, http://zenodo.org/record/3673375, {"references":["Souza-Kury LAi Lemos de Castro A. 1991. The genus Circoniscus Pearsei 1917 in Brazili with a description of three new species (Isopoda Oniscidea Scleropactidae). Trop Zool. 4: 45 - 64. doi: 10.1080 / 03946975.1991.10539474.","Schultz GA. 1995. Terrestrial isopod crustaceans (Oniscidea) from Paraguay with definition of a new family. Rev Suisse Zool. 102: 387 - 424. doi: 10.5962 / bhl. part. 80471.","Schmidt C. 2007. Revision of the Neotropical Scleropactidae (Crustacea: Oniscidea). Zool J Linn Soc. 151: 1 - 339. doi: 10.1111 / j. 1096 - 3642.2007.00286. x.","Campos-Filho ISi Araujo PBi Bichuette MEi Trajano Ei Taiti S. 2014. Terrestrial isopods (Crustacea: Isopoda: Oniscidea) from Brazilian caves. Zool J Linn Soc. 172: 360 - 425. doi: 10.1111 / zoj. 2014.172. issue- 2.","Arcangeli A. 1931. Circoniscus bezzii Arc. i nuova specie di isopodo terrestre del Brasile. B Zool. 11: 115 - 122. doi: 10.1080 / 11250003109434856.","Leistikow Ai Wagele JW. 1999. Checklist of terrestrial isopods of the new world (Crustaceai Isopodai Oniscidea). Rev Bras Zool. 16: 1 - 72. doi: 10.1590 / S 0101 - 81751999000100001.","Schmalfuss H. 2003. World catalog of terrestrial isopods (Isopoda: Oniscidea). Stuttgart Beit Nat. 654: 1 - 341.","Campos-Filho ISi Cardoso GMi Aguiar JO. 2018 a. Catalogue of terrestrial isopods (Crustaceai Isopodai Oniscidea) from Brazil: an update with some considerations. Nauplius. 26: e 2018038. doi: 10.1590 / 2358 - 2936 e 2018038."]}

Venezillo congener (Budde-Lundı 1904) Material examined Goi��sı S��o Domingos: Suspirinho caveı 25 April 2012ı leg. DM Schimonskyı JE Gall��o and CS Fernandes (LES: 1♀ ı 6368; 13ı 6371); 1♀ ı same localityı 9 February 2012ı leg. DM Schimonskyı JE Gall��o and LB Sim��es (LES 6374); 13ı Suspir��o caveı 9 February 2012ı leg. DM Schimonskyı JE Gall��o and LB Sim��es (LES 6379); Lapa do Ang��lica caveı 20 April 2011ı leg. ME Bichuetteı PP Rizzatoı JE Gall��o (LES: 1♀ ı 6382; 2♀ ı 6383; 13ı 1♀ ı 6384; 13ı 1♀ ı 6385); same locality as previousı 18 June 2012ı leg. DM Schimonskyı JE Gall��o and LB Sim��es (LES: 23ı 1♀ ı 6397; 1♀ ı 6398); 1♀ ı same locality and collectors as previousı 10 February 2012 (LES 6413); 1♀ ı same locality as previous (epigean)ı 31 October 2012ı ME Bichuetteı JE Gall��oı LB Sim��esı CS Fernandes and T Zepon (LES 6406); 7♀ ı Caverna Bezerra cave (epigean)ı 12 February 2012ı leg. DM Schimonskyı JE Gall��o and LB Sim��es (LES 6393); same locality and collectors as previous (Epigean)ı 19 June 2012 (LES: 13ı 6399; 13ı 6400; 13ı 6403; 1♀ ı 6414). Distribution Many places in the states of Goi��s and Mato Grosso do Sul (Van Name 1936; Vilela 1971 ı Campos-Filho et al. 2019). Remarks This species was recently re-described (Campos-Filho et al. 2019). As mentioned by the authorsı this species inhabits decaying and humid leaf litterı and it was collected in- and outside cavesı supporting its trogophilic status., Published as part of Camile Sorbo Fernandes, Ivanklin Soares Campos-Filho, Paula Beatriz Araujo & Bichuette, Maria Elina, 2019, Synopsis of terrestrial isopods (Crustacea: Isopoda: Oniscidea) from Brazilian caves�� with emphasis on new records from north�� midwest�� northeast and southeast regions, pp. 1095-1129 in Journal of Natural History 53 (17) on page 1120, DOI: 10.1080/00222933.2019.1634225, http://zenodo.org/record/3673375, {"references":["Van Name WG. 1936. The American land and freshwater isopod Crustacea. B Am Mus Nat Hist. 71: 1 - 535.","Vilela Ei Kudo Hi Loureiro M. 1971. Oniscoides de Douradosi Estado de Mato Grosso. Seiva. 31: 183 - 189.","Campos-Filho ISi Fernandes CSi Cardoso GMi Bichuette MEi Aguiar JOi Taiti S. 2019. Two new species and new records of terrestrial isopods (Crustaceai Isopodai Oniscidea) from Brazilian caves. Zootaxa. 4564 (2): 422 - 448. doi: 10.11646 / zootaxa. 4564.2.6"]}

Zimmermann, Bianca Laís, Campos-Filho, Ivanklin Soares, Cardoso, Giovanna Monticelli, Santos, Sandro, Aguiar, José Otávio, Araujo, Paula Beatriz (2018): Two new species of Atlantoscia Ferrara & Taiti, 1981 (Isopoda: Oniscidea: Philosciidae) from southern Brazil described in the light of integrative taxonomy. Zootaxa 4482 (3): 551-565, DOI: https://doi.org/10.11646/zootaxa.4482.3.7

Genus Atlantoscia Ferrara & Taiti, 1981 Type species. Atlantoscia alceui Ferrara & Taiti, 1981 by monotypy. Diagnosis. See Araujo & Leistikow (1999) and Campos-Filho et al. (2013)., Published as part of Zimmermann, Bianca La��s, Campos-Filho, Ivanklin Soares, Cardoso, Giovanna Monticelli, Santos, Sandro, Aguiar, Jos�� Ot��vio & Araujo, Paula Beatriz, 2018, Two new species of Atlantoscia Ferrara & Taiti, 1981 (Isopoda: Oniscidea: Philosciidae) from southern Brazil described in the light of integrative taxonomy, pp. 551-565 in Zootaxa 4482 (3) on page 553, DOI: 10.11646/zootaxa.4482.3.7, http://zenodo.org/record/1440803, {"references":["Ferrara, F. & Taiti, S. (1981) Terrestrial isopods from Ascension Island. Monitore Zoologico Italiano. Supplemento, 14 (1), 189 - 198. https: // doi. org / 10.1080 / 03749444.1981.10736621","Araujo, P. B. & Leistikow, A. (1999) Philosciids with pleopodal lungs from Brazil, with description of a new species (Crustacea, Isopoda). Contributions to Zoology, 68 (2), 109 - 141.","Campos-Filho, I. S., Lisboa, J. T. & Araujo, P. B. (2013) Review of Atlantoscia Ferrara & Taiti, 1981 (Crustacea: Isopoda: Oniscidea: Philosciidae) with new records and new species. Organisms Diversity & Evolution, 13 (3), 463 - 483. https: // doi. org / 10.1007 / s 13127 - 013 - 0124 - 8"]}

Atlantoscia australis Campos-Filho, Cardoso & Araujo sp. nov. Figs 4–6 Zoobank. urn:lsid:zoobank.org:act:39 BAE 885-6EE1-46A6-AAE0-60 CAFC 82FA41. Etymology. The new species is named after the Triangulum australe constellation, located in the far of the southern hemisphere sky. This constellation represents the state of Santa Catarina in the Brazilian National flag. Material examined. Holotype: Male, Santa Catarina, Santa Rosa de Lima, 28°06’09”S 49°07’35”W, alt. 225 m., 25 Jun 2012, leg. P.B. Araujo and B.L. Zimmermann (MZUSP 36764). Paratypes: 2 males (one in micropreparations), 9 females (MZUSP 36765), same data as holotype. Description. Maximum body length: male 5.5 mm, female 8.5 mm. Color light brown; antenna strongly pigmented; cephalon with irregular unpigmented spots; pereon with unpigmented spots on median and paramedian regions, epimera more pigmented with one or two unpigmented spots; pleonites 1 and 2 with two unpigmented spots, pleonites 3 and 4 with one unpigmented spot on median region and two unpigmented spots laterally, pleonite 5 more pigmented with one unpigmented spot on media region; telson with three unpigmented spots. Body outline as in Fig. 4A. Cephalon (Fig. 4B) with lateral lobes not developed, frontal line absent, suprantennal line bent downwards on middle. Dorsum covered with elongated triangular scale-setae (Fig. 4C). Pereon (Fig. 4A) with pereonite 1 epimera directed frontwards, not surpassing eyes; pereonites 5–7 epimera gradually directed backwards; pereonite 7 with posterior margin arched. Pereonites with glandular pores on lateral margin. Noduli laterales coordinates as in Fig. 4D and E. Pleon (Fig. 4A, F) narrower than pereon; pleonites 3–5 epimera not well-developed, directed backwards. Telson (Fig. 4F) triangular, lateral sides almost straight. Antennula (Fig. 4G) of three articles, distal article longest bearing eight lateral aesthetascs in four rows plus apical pair. Antenna (Fig. 4H) reaching pereonite 4 when extended backwards; flagellum of three articles, proximal and distal articles subequal in length, medial and distal articles bearing lateral aesthetascs, distal organ about half of length of distal article of flagellum. Mandibles with dense cushion of setae, molar penicil of about five branches, left mandible (Fig. 5A) with 2+1 penicils, right mandible (Fig. 5B) of 1+1 penicils. Maxillula (Fig. 5C) inner endite with distal margin rounded bearing two hairy penicils; outer endite of 4+6 teeth, five of them with cleft at apex. Maxilla (Fig. 5D) inner lobe rounded bearing thick setae; outer lobe three times as wide as inner lobe covered with thin setae, distal margin sinuous. Maxilliped (Fig. 5E) basis subrectangular, palp with two long setae on proximal article, endite with distal margin rounded bearing two hook-like setae. Pereopods (Fig. 6A, B) rather slender bearing fringe of scales on merus and carpus; carpus 1 with transverse antennal grooming brush and distal seta double fringed at apex. Dactylus (Fig. 6A) of two claws, inner claw not surpassing outer claw; ungual seta and dactylar organ simple, not surpassing outer claw. Uropod (Fig. 4F) protopod subquadrangular, protopod and exopod grooved on outer margin bearing many glandular pores; exopod about twice as long as endopod; endopod inserted proximally. Respiratory areas of floridana -type on pleopod exopods. Male. Genital papilla (Fig. 6C) with triangular ventral shield, distal papilla bearing two subapical orifices. Pleopod 1 (Fix. 6D) exopod heart-shaped, outer margin concave bearing one small seta; endopod longer than exopod, distal portion slightly bent outwards bearing small setae on median margin and serrate plaque. Pleopod 2 (Fig. 6E) exopod triangular, outer margin concave bearing five small setae; endopod slightly longer than endopod. Pleopod 3 (Fig. 6F) exopod triangular, outer margin concave bearing three setae. Pleopod 4 (Fig. 6G) exopod triangular, outer margin slightly concave bearing four setae. Pleopod 5 (Fig. 6H) exopod triangular, outer margin sinuous bearing three setae. Remarks. Atlantoscia australis sp. nov. differs from A. antennamaculata sp. nov. in having the distal portion of the telson triangular (vs. narrower in A. antennamaculata), antennula with eight aesthetascs (vs. 11 in A. antennamaculata), male pleopod 1 exopod with distal portion not elongated (vs. elongated in A. antennamaculata), male pleopod 1 endopod with distal portion slightly directed outwards (vs. bent outwards in A. antennamaculata), and male pleopod 5 exopod triangular (vs. rhomboid in A. antennamaculata). Phylogenetic Analysis. All specimens of A. antennamaculata sp. nov. and A. australis sp. nov. presented a single mitochondrial haplotype for the COI sequences. In addition, no intraspecific variation was found in the 18S sequences for both species. For this reason, the new species are represented, each one, by a single sequence in the phylogenetic trees. Final COI alignment consists of 640 bp, with 203 variable sites and 170 parsimony informative sites. On the other hand, final 18S alignment consists of 434 bp (including gaps), with 33 variable sites and 22 parsimony informative sites. Maximum likelihood and Bayesian analyses produced trees with similar topologies (data not shown). Although the phylogenies generated with mitochondrial COI (Fig. 7) and 18S nuclear (Fig. 8) sequences did not exhibit the same groupings among the Atlantoscia species, they were congruent regarding the taxa of interest; that is, both markers support the validity of the two new species of Atlantoscia. Some disagreement between the molecular markers is expected due to the different evolutionary rates between them. More specifically, the short size of the nuclear sequences added to their lower evolutionary rates may have generated the lack of congruence among some of the generated clades when compared to the mitochondrial tree. These results, however, do not interfere with the validity of the new species described herein. Sequences were deposited in GenBank under the accession numbers MH047282 to MH047285.3 The average mtDNA genetic distance among species of Atlantoscia was 13.9%, ranging from 13.4 to 20%. The genetic distance between A. antennamaculata sp. nov. and the other congeneric species was 15.4%; for A. australis sp. nov. this value was 17% (Table 1). In relation to the conserved nuclear sequences, the average genetic distance among Atlantoscia species was 0.8%. The genetic distances between Atlantoscia species and the new species A. antennamaculata sp. nov. and A. australis sp. nov. were 1.2% and 0.7%, respectively (Table 2). Species delimitation. The likelihood of the null model in the GMYC analysis was significantly lower than the maximum likelihood of species delimitation (38.31 versus 45.11, ratio = 14.96, P = 0.001). As the null model assumes that all individuals within the sample belong to a single species, we reject this model. The GMYC analyses based on the COI dataset indicated the presence of 7 ML entities. Meanwhile, ABGD analysis with JC69 and K2P models produced ten recursive partitions, which recovered seven groups in all cases. These results support those of the phylogenetic analysis and confirmed the taxonomic validity of the currently recognized species of Atlantoscia.