1. Cutting Edge: l-Arginine Transfer from Antigen-Presenting Cells Sustains CD4 + T Cell Viability and Proliferation.
- Author
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Crowther RR, Schmidt SM, Lange SM, McKell MC, Robillard MC, Zhao J, Haffey WD, Wyder MA, Greis KD, Setchell KDR, and Qualls JE
- Subjects
- Animals, Arginine biosynthesis, Argininosuccinic Aciduria etiology, Argininosuccinic Aciduria metabolism, Biological Transport, Biomarkers, Cell Proliferation, Cell Survival immunology, Flow Cytometry, Immunophenotyping, Lymphocyte Activation genetics, Mice, Mice, Transgenic, Antigen-Presenting Cells immunology, Antigen-Presenting Cells metabolism, Arginine metabolism, CD4-Positive T-Lymphocytes immunology, CD4-Positive T-Lymphocytes metabolism, Lymphocyte Activation immunology
- Abstract
Metabolomics analyses suggest changes in amino acid abundance, particularly l-arginine (L-ARG), occur in patients with tuberculosis. Immune cells require L-ARG to fuel effector functions following infection. We have previously described an L-ARG synthesis pathway in immune cells; however, its role in APCs has yet to be uncovered. Using a coculture system with mycobacterial-specific CD4
+ T cells, we show APC L-ARG synthesis supported T cell viability and proliferation, and activated T cells contained APC-derived L-ARG. We hypothesize that APCs supply L-ARG to support T cell activation under nutrient-limiting conditions. This work expands the current model of APC-T cell interactions and provides insight into the effects of nutrient availability in immune cells., (Copyright © 2022 by The American Association of Immunologists, Inc.)- Published
- 2022
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