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Made available in DSpace on 2020-12-12T02:21:44Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01 In view of the established climate change scenario and the consequent changes in global temperature, it is essential to study its effects on animal spermatogenesis. Therefore, the aim of this study was to verify the duration of spermatogenesis at different temperatures. For this purpose, 96 male and adult specimens of Astyanax altiparanae were kept in a closed circulation system with water temperature stabilized at 27 °C and 32 °C. Subsequently, the specimens received pulses of BrdU (bromodeoxyuridine) at a concentration of 100 mg/kg/day for 2 consecutive days, and the samples were collected daily for a period of 15 days. Their testes were removed, fixed, processed in historesin, and sectioned in 3 μm, submitted to hematoxylin/eosin staining and to bromodeoxyuridine immunodetection. Partial results of the optimum temperature experiments allowed the classification of A. altiparanae spermatogenic cells in Aund, Adiff, and type B spermatogonia, spermatocytes, spermatids, and spermatozoa. The duration of spermatogenesis was determined as approximately 6 days for animals at a temperature of 27 °C and 1 day for animals at 32 °C. The elevated temperature was also responsible for increasing cell proliferation, resulting in an increase in the number of spermatocytes, spermatids, spermatozoa, and cell death (cell pyknotic). The duration of spermatogenesis in A. altiparanae was directly affected by the elevated water temperature, causing a reduction in the estimated time of spermatogenesis. Departamento de Biologia e Zootecnia. L.I.NEO - Laboratory of Neotropical Ichthyology UNESP - Universidade Estadual Paulista “Júlio de Mesquita Filho” Câmpus de Ilha Solteira, Rua Monção, n 226, 15385-000 Programa de Pós-Graduação em Ciências Biológicas (Zoologia) Instituto de Biociências de Botucatu (UNESP - IBB), R. Prof. Dr. Antonio Celso Wagner Zanin, 250, 18618-689 Instituto de Estudo em Saúde e Biologicas (IESB) Research Group of Reproduction on Amazon Fish (GERPA/LaNec) UNIFESSPA - Universidade Federal do Sul e Sudeste do Para, Folha 31, Quadra 07, Lote especial s/n, 68.507-590 Departamento de Morfologia Reproductive and Molecular Biology Group UNESP - Universidade Estadual Paulista “Júlio de Mesquita Filho” Câmpus de Botucatu Instituto de Biociências de Botucatu, R. Prof.Dr. Antonio Celso Wagner Zanin, 250, 18618-689 Departamento de Biologia e Zootecnia. L.I.NEO - Laboratory of Neotropical Ichthyology UNESP - Universidade Estadual Paulista “Júlio de Mesquita Filho” Câmpus de Ilha Solteira, Rua Monção, n 226, 15385-000 Programa de Pós-Graduação em Ciências Biológicas (Zoologia) Instituto de Biociências de Botucatu (UNESP - IBB), R. Prof. Dr. Antonio Celso Wagner Zanin, 250, 18618-689 Departamento de Morfologia Reproductive and Molecular Biology Group UNESP - Universidade Estadual Paulista “Júlio de Mesquita Filho” Câmpus de Botucatu Instituto de Biociências de Botucatu, R. Prof.Dr. Antonio Celso Wagner Zanin, 250, 18618-689

The exposure of adult fish to warm or high temperatures is known to impair reproduction, yet the long-term reproductive impacts for treatments at early life are not well clarified. This study aimed to evaluate the effects of warm temperature (WT) during juvenile stage on gonad maturation, gamete quality, and offspring thermotolerance in rainbow trout. While the comparison of basic reproductive parameters in WT females did not reveal any kind of impairment, many WT males showed an atrophied, undeveloped gonad, or a smaller testis with lower milt volume; sperm quality parameters in WT males and deformity rates in the respective progeny were also highly affected. However, despite of such negative effects, many of the remaining progeny presented better rates of survival and growth when exposed to the same conditions as those of parental fish (WT), suggesting that thermal stress in parr stage males elicited intergenerational thermotolerance after a single generation. The present results support that prolonged warming stress during early life stages can adversely affect key reproductive aspects, but contrastingly increase offspring performance at upper thermal ranges. These findings have implications on the capacity of fish to adapt and to cope with global warming. Fil: Butzge, Arno Juliano. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil Fil: Yoshinaga, Tulio Teruo. Universidade de Sao Paulo; Brasil Fil: Moreno Acosta, Omar David. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina Fil: Fernandino, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina Fil: Sanches, Eduardo Antônio. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil Fil: Tabata, Yara Aiko. Salmonid Experimental Station At Campos Do Jordão; Brasil Fil: de Oliveira, Claudio. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil Fil: Takahashi, Neuza Sumico. Centro de Pesquisa de Aquicultura; Brasil Fil: Hattori, Ricardo Shohei. Salmonid Experimental Station at Campos do Jordão; Brasil

Made available in DSpace on 2021-06-25T10:54:33Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-06-01 Centers for Disease Control and Prevention Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Fundação para o Desenvolvimento da UNESP (FUNDUNESP) Universidade Estadual Paulista ASCRS Research Foundation Anti-Müllerian hormone (Amh) is a member of the transforming growth factor-β (Tgf-β) superfamily required in the regression of Müllerian ducts during gonadal sex differentiation of higher vertebrates. Teleost fish lack Müllerian ducts, but identified Amh orthologs have been shown to exert crucial functions during sex determination and differentiation of several species of teleosts. However, the function of Amh during gametogenesis in adult fish remains poorly investigated. Therefore, to expand present knowledge on the role of Amh in teleosts, the present study aimed to isolate and clone full-length amh cDNA in the common carp, Cyprinus carpio, and examine its expression levels throughout the male reproductive cycle and in response to different hormone treatments of testicular explants. Molecular cloning and characterization showed that the common carp Amh precursor amino acid sequence shared common features to other fish Amh precursors, including a conserved C-terminus (Tgf-β domain) and a double proteolytic cleavage site (R-X-X-R-X-X-R) upstream to the Tgf-β domain. Expression analysis showed amh dimorphic expression in the adult gonads with higher expression in the testes than ovaries. In testes, amh mRNA was detected in Sertoli cells contacting different types of germ cells, although the expression was greatest in Sertoli cells associated with type A undifferentiated spermatogonia. Expression analysis during the reproductive cycle showed that amh transcripts were down-regulated during the developing phase, which is characterized by an increased proliferation of type A undifferentiated spermatogonia and Sertoli cells and appearance of spermatocytes (meiosis) in the testes. Furthermore, ex vivo experiments showed that a 7 day exposure to Fsh or estrogens was required to decrease amh mRNA levels in common carp testicular explants. In summary, this study provided information on the molecular characterization and transcript abundance of amh in common carp adult testes. Altogether, these data will be useful for further investigations on sex determination and differentiation in this species, and also to improved strategies for improved carp aquaculture, such as inhibiting precocious maturation of males. Aquaculture Program (CAUNESP) São Paulo State University (UNESP), Jaboticabal Reproductive and Molecular Biology Group Department of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP), Botucatu Faculty of Veterinary Medicine and Animal Science San Martín University Foundation (FUSM) Department of Cell Biology and Histology Faculty of Biology Universidad de Murcia IMIB-Arrixaca Department of Orthopaedics Muscle skeletal Research West Virginia University Aquaculture Program (CAUNESP) São Paulo State University (UNESP), Jaboticabal Reproductive and Molecular Biology Group Department of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP), Botucatu

The exposure of adult fish to warm or high temperatures is known to impair reproduction, yet the long-term reproductive impacts for treatments at early life are not well clarified. This study aimed to evaluate the effects of warm temperature (WT) during juvenile stage on gonad maturation, gamete quality, and offspring thermotolerance in rainbow trout. While the comparison of basic reproductive parameters in WT females did not reveal any kind of impairment, many WT males showed an atrophied, undeveloped gonad, or a smaller testis with lower milt volume; sperm quality parameters in WT males and deformity rates in the respective progeny were also highly affected. However, despite of such negative effects, many of the remaining progenies presented better rates of survival and growth when exposed to the same conditions as those of parental fish (WT), suggesting that thermal stress in parr stage males elicited intergenerational thermoresistance after a single generation . The present results support that prolonged warming stress during early life stages can adversely affect key reproductive aspects, but constrastingly increase offspring performance at upper thermal ranges. These findings have implications on the capacity of fish to adapt and to cope with global warming.

Made available in DSpace on 2020-12-10T19:59:34Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-27 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Albinism is the most common color variation described in fish and is characterized by a white or yellow phenotype according to the species. In rainbow trout Oncorhynchus mykiss, aside from yellow-albino phenotypes, cobalt blue variants with autosomal, recessive inheritance have also been reported. In this study, we investigated the inheritance pattern and chromatophores distribution/abundance of cobalt blue trouts obtained from a local fish farm. Based on crosses with wild-type and dominant yellow-albino lines, we could infer that cobalt blue are dominant over wild-type and co-dominant in relation to yellow-albino phenotype, resulting in a fourth phenotype: the white-albino. Analysis of chromatophores revealed that cobalt blue trouts present melanophores, as the wild-type, and a reduced number of xanthophores. As regards to the white-albino phenotype, they were not only devoid of melanophores but also presented a reduced number of xanthophores. Cobalt blue and white-albino trouts also presented reduced body weight and a smaller pituitary gland compared to wild-type and yellow-albino phenotypes. The transcription levels of tshb and trh were up regulated in cobalt blue compared to wild type, suggesting the involvement of thyroid hormone in the expression of blue color. These phenotypes represent useful models for research on body pigmentation in salmonids and on the mechanisms behind endocrine control of color patterning. Agencia Paulista Tecnol Agronegocios, Secretaria Agr, Unidade Pesquisa & Desenvolvimento Campos Jordao, Salmonid Expt Stn Campos Jordao, Sao Paulo, Brazil Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Surg, Sao Paulo, Brazil Sao Paulo State Univ, Grad Program Biol Sci Genet, Inst Biosci Botucatu, Botucatu, SP, Brazil Tokyo Univ Marine Sci & Technol, Dept Marine Biosci, Tokyo, Japan Puriaqua Limitada, Sao Paulo, Brazil Agencia Paulista Tecnol Agronegocios, Secretaria Agr, Sao Paulo Fisheries Inst, Sao Paulo, Brazil Sao Paulo State Univ, Grad Program Biol Sci Genet, Inst Biosci Botucatu, Botucatu, SP, Brazil FAPESP: 2013/17612-9 FAPESP: 2014/00569-6

Made available in DSpace on 2021-06-25T15:01:12Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-05-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Germ cell transplantation and testis graft represent promising biotechnologies that can be applied for the reproduction of commercial or endangered species. However, mechanisms of rejection from the host immune system might remove the transplanted donor cells/tissues and limit the surrogate production of gametes. In this work, we administered emulsion containing-immunosuppressants to verify whether they are capable to prevent immune rejection and promote survival of testis allografts in rainbow trout. In the first part of this study, we demonstrated in vitro that tacrolimus and cyclosporine were able to affect viability, inhibit leucocyte proliferation, and suppress il2 expression in vitro. In in vivo experiments, both doses of tacrolimus (0.5 and 1.5 mg/kg) and the lower dose of cyclosporine (20 mg/kg) significantly inhibited the expression of il2 in head kidney, three days post-injection. A higher dose of cyclosporine (40 mg/kg) was able to inhibit il2 expression for up to seven days post-injection. In the second part, testis allografts were conducted in fish treated weekly with emulsion containing-tacrolimus. Immunohistochemical, conventional histology, and qRT-PCR (vasa) analysis demonstrated the presence of spermatogonial cells by the fifth week, in animals treated with 0.5 mg/kg of tacrolimus similar as found in autografted group. In the group treated with the highest tacrolimus dose (1.5 mg/kg) and in the non-treated group (without immunosuppressant), no germ cells or their respective markers were detected. il2 expression in head kidney was also suppressed in grafted animals treated with tacrolimus compared to nontreated group. These results suggest that tacrolimus may be a promising immunosuppressant for testis allografts or germ cell transplantation in rainbow trout. Co-administration combining tacrolimus (at lower dose) with other immunosuppressive drugs for inhibiting other activation pathways of the immune system, as performed in human organ transplantation, could be an alternative approach to optimize the immunosuppressive effects in host organisms. Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Surg, BR-05508270 Sao Paulo, Brazil Sao Paulo State Univ, Inst Biosci Botucatu, Dept Morphol, BR-18618689 Botucatu, SP, Brazil Univ Sao Paulo, Biomed Sci Inst, Interunit Biotechnol Program, BR-05508900 Sao Paulo, Brazil Univ Sao Paulo, Cell & Mol Therapy Ctr, Sch Med, Internal Med Dept,NUCEL, BR-05360130 Sao Paulo, Brazil Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Pathol, BR-05508270 Sao Paulo, Brazil UPD CJ APTA SAA, Salmonid Expt Stn Campos do Jordao, BR-12460000 Campos Do Jordao, Brazil Sao Paulo State Univ, Inst Biosci Botucatu, Dept Morphol, BR-18618689 Botucatu, SP, Brazil FAPESP: 2013/17612-9 FAPESP: 2015/20762-8 CNPq: 404583/2016-4 CAPES: 001

Albinism is the most common color variation described in fish and is characterized by a white or yellow phenotype according to the species. In rainbow troutOncorhynchus mykiss, aside from yellow-albino phenotypes, cobalt blue variants with autosomal, recessive inheritance have also been reported. In this study, we investigated the inheritance pattern and chromatophores distribution/abundance of cobalt blue trouts obtained from a local fish farm. Based on crosses with wild-type and dominant yellow-albino lines, we could infer that cobalt blue are dominant over wild-type and co-dominant in relation to yellow-albino phenotype, resulting in a fourth phenotype: the white-albino. Analysis of chromatophores revealed that cobalt blue trouts present melanophores, as the wild-type, and a reduced number of xanthophores. As regards to the white-albino phenotype, they were not only devoid of melanophores but also presented a reduced number of xanthophores. Cobalt blue and white-albino trouts also presented a more elongated body shape and, most remarkably, a smaller pituitary gland compared to wild-type and yellow-albino, suggesting that the allele for blue color is somehow linked with this abnormal pituitary phenotype. These phenotypes represent interesting models for research on body pigmentation in salmonids and on the mechanisms behind endocrine control of color patterning.

Cortisol is the major endocrine factor mediating the inhibitory effects of stress on vertebrate reproduction. It is well known that cortisol affects reproduction by interacting with the hypothalamic&ndash, pituitary&ndash, gonads axis, leading to downstream inhibitory and stimulatory effects on gonads. However, the mechanisms are not fully understood. In this study, we provide novel data demonstrating the stimulatory effects of cortisol on spermatogenesis using an ex vivo organ culture system. The results revealed that cortisol treatment did not modulate basal androgen production, but it influenced transcript levels of a selected number of genes involved in the zebrafish testicular function ar (androgen receptor), star (steroidogenic acute regulatory), cyp17a1 (17&alpha, hydroxylase/17,20 lyase/17,20 desmolase), cyp11a2 (cytochrome P450, family 11, subfamily A, polypeptide 2), hsd11b2 (11-beta hydroxysteroid dehydrogenase), cyp2k22 (cytochrome P450, family 2, subfamily K, polypeptide 22), fkbp5 (FKBP prolyl isomerase 5), gr&alpha, (glucocorticoid receptor alpha), and gr&beta, (glucocorticoid receptor beta) in a short-term culture. We also showed that cortisol stimulates spermatogonial proliferation and differentiation in an androgen independent manner as well as promoting meiosis and spermiogenesis by increasing the number of spermatozoa in the testes. Moreover, we demonstrated that concomitant treatment with RU 486, a potent glucocorticoid receptor (Gr) antagonist, did not affect the cortisol effects on spermatogonial differentiation but blocked the induced effects on meiosis and spermiogenesis. Supporting the Gr-mediated effects, RU 486 nullified the cortisol-induced expression of sycp3l (synaptonemal complex protein 3), a marker for the meiotic prophase that encodes a component of the synaptonemal complex. This is consistent with in silico analysis that found 10 putative GREs (glucocorticoid response elements) upstream of the zebrafish sycp3l. Finally, we also showed that gr&alpha, mRNA is expressed in Sertoli and Leydig cells, but also in several types of germ cells, including spermatogonia and spermatocytes. Altogether, this evidence indicates that cortisol exerts paracrine roles in the zebrafish testicular function and spermatogenesis, highlighting its effects on spermatogonial differentiation, meiosis, and spermiogenesis.

Gonadotropin releasing hormone (GnRH) is one of the key players of brain-pituitary-gonad axis, exerting overall control over vertebrate reproduction. In zebrafish, two variants were characterized and named as Gnrh2 and Gnrh3. In this species, Gnrh3, the hypohysiotropic form, is expressed by neurons of the olfactory-retinal system, where it is related with food detection, intra/interspecific recognition, visual acuity and retinal processing modulation. Previous studies have reported the presence of Gnrh receptors in the zebrafish retina, but not yet in the zebrafish olfactory epithelium. The current study analyzed the presence of gnrh2 and gnrh3, their receptors (gnrhr 1,2,3 and 4) and gnih (gonadotropin inhibitory hormone) transcripts, as well as the Gnrh3 protein in the olfactory epithelium (OE), olfactory bulb (OB), retina and ovary during zebrafish ovarian maturation. We found an increase of gnrh receptors transcripts in the OE at the final stages of ovarian maturation. In the OE, Gnrh3 protein was detected in the olfactory receptor neurons cilia and in the olfactory nerve fibers. Interestingly, in the OB, we found an inverse expression pattern between gnih and gnrh3. In the retina, gnrhr4 mRNA was found in the nuclei of amacrine, bipolar, and ganglion cells next to Gnrh3 positive fibers. In the ovary, gnrh3, gnrhr2 and gnrhr4 transcripts were found in perinucleolar oocytes, while gnih in oocytes at the cortical alveolus stage. Our results suggested that Gnrh/Gnih elements are involved in the neuromodulation of the sensorial system particularly at the final stages of maturation, playing also a paracrine role in the ovary. Fil: Corchuelo, Sheryll. Universidade de Sao Paulo; Brasil Fil: Martinez, Emanuel R. M.. Universidade de Sao Paulo; Brasil Fil: Butzge, Arno J.. Universidade de Sao Paulo; Brasil Fil: Doretto, Lucas B.. Universidade de Sao Paulo; Brasil Fil: Ricci, Juliana M.B.. Universidade de Sao Paulo; Brasil Fil: Valentin, Fernanda N.. Universidade de Sao Paulo; Brasil Fil: Nakaghi, Laura S.O.. Universidade de Sao Paulo; Brasil Fil: Somoza, Gustavo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina Fil: Nóbrega, Rafael H.. Universidade de Sao Paulo; Brasil

The aim study it had as objective to evaluate the effect of different ammonia levels, on the parameters of sperm motility, the artificial fertilization and the early development of jundia (Rhamdia quelen; Siluriformes, Heptapteridae). The semen was activated using the dilution of 1:70 (semen:water) and evaluated by the CASA method. It was considered as treatment water contending 0,06; 0,28; 0,50; 0,77 and 0,84 mg of total ammonia.water-1 L, so for sperm activation as for fertilization and incubation of the oocytes. The incubation was composed for a recirculation system of 250L for each treatment, with four incubators, being that each incubator approximately had useful volume of 2,5L with 3,800 eggs. Had been evaluated the parameters of sperm motility, fertilization rate, thermal unit accumulated for beginning and ending of the hatching, hatching rate, embryonic and percentile development of normal larvae. The results had been submitted the analysis of variance and the analysis of regression to a 5% of probability level. The ammonia levels gifts in the water did not interfere on the evaluated parameters (p> 0,05), except about to the early embryonic development, where the embryos displayed to the biggest levels of contamination for ammonia had presented greaters (p>0,05) closing of the blastopore rates. In general way, the presence of until 0,84mg of total ammonia. Water-1 L speeds up the embryonic early development but they do not interfere with the fertilization and hatching rates. O presente estudo teve como objetivo avaliar os efeitos de diferentes níveis de amônia, sobre os parâmetros de motilidade espermática, a fertilização artificial e o desenvolvimento inicial do jundiá cinza (Rhamdia quelen; Siluriformes, Heptapteridae). O sêmen foi ativado utilizando a diluição de 1:70 (sêmen:água) e avaliado pelo método CASA. Foi considerado como tratamento água contendo 0,06; 0,28; 0,50; 0,77 e 0,84 mg de amônia total.L de água-1, tanto para ativação espermática quanto para fertilização e incubação dos ovócitos. A incubação era composta por um sistema de recirculação de 250L para cada tratamento, com quatro incubadoras, sendo que cada incubadora tinha volume útil de 2,5L com aproximadamente 3.800 ovos. Foram avaliados os parâmetros de motilidade espermática, de taxa de fertilização, unidade térmica acumulada para início e término da eclosão, taxa de eclosão, desenvolvimento embrionário e percentual de larvas normais. Os resultados foram submetidos a análise de variância e a análise de regressão a um nível de 5% de probabilidade. Os níveis de amônia presentes na água não interferiram sobre os parâmetros avaliados (p>0,05), exceto quanto ao desenvolvimento embrionário inicial, onde os embriões expostos aos maiores níveis de contaminação por amônia apresentaram maiores (p

This study evaluated the effect of feed supplementation (at doses of 0, 50, 100, 150, and 200 mg/kg) with SALUTO®, a microencapsulated blend of essential oils including carvacrol, cinnamaldehyde, 1,8-cineol, and pepper oleoresin, in Nile tilapia (Oreochromis niloticus) broodstock on reproductive and growth parameters during the initial phase of rearing. The growth parameters, somatic indexes and gonadal and hepatic parameters were analyzed. Quantitative parameters of growth, ovary fat and crude protein were not affected by supplementation of the additive. However, an increase in crude protein in the female liver was observed in correlation with increased essential oil levels. The female hepatosomatic index was also correlated with supplementation of essential oils. Feeding Nile tilapia broodstock diets containing an essential oil compound (SALUTO®) affects only the females, promoting an increase in liver protein inclusion and hepatosomatic index without impairing performance.

The aim study it had as objective to evaluate the effect of different ammonia levels, on the parameters of sperm motility, the artificial fertilization and the early development of jundia ( Rhamdia quelen ; Siluriformes, Heptapteridae). The semen was activated using the dilution of 1:70 (semen:water) and evaluated by the CASA method. It was considered as treatment water contending 0,06; 0,28; 0,50; 0,77 and 0,84 mg of total ammonia.water-1 L, so for sperm activation as for fertilization and incubation of the oocytes. The incubation was composed for a recirculation system of 250L for each treatment, with four incubators, being that each incubator approximately had useful volume of 2,5L with 3,800 eggs. Had been evaluated the parameters of sperm motility, fertilization rate, thermal unit accumulated for beginning and ending of the hatching, hatching rate, embryonic and percentile development of normal larvae. The results had been submitted the analysis of variance and the analysis of regression to a 5% of probability level. The ammonia levels gifts in the water did not interfere on the evaluated parameters (p> 0,05), except about to the early embryonic development, where the embryos displayed to the biggest levels of contamination for ammonia had presented greaters (p>0,05) closing of the blastopore rates. In general way, the presence of until 0,84mg of total ammonia. Water-1 L speeds up the embryonic early development but they do not interfere with the fertilization and hatching rates.