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The current work aimed to analyze, morphologically, statistically and molecularly, oocysts shed from plumbeous pigeons Patagioenas plumbea Vieillot, 1818 from a locality at 2197m of altitude near the Agulhas Negras peak, the highest point of the State of Rio de Janeiro, southeastern Brazil. The oocysts were extremely polymorphic, being subspheroidal, ovoidal or ellipsoidal, in addition to having the random presence/absence of characteristic features associated with the oocyst wall, such as micropyle, micropyle cap, lateral micropyle and outer veil/rough wall. Linear regression confirmed the extreme polymorphism of oocysts, showing that if all combinations of taxonomic characters in oocysts (morphotypes) were overestimated, 19 different species could be identified/described. In contrast, the means comparison analysis between oocysts with presence/absence of characteristic features and the histograms showed equivalences and regularity in the distribution in the classes of measures, which indicate the presence of a single species in the measured oocysts. Molecular analyzes was performed from the isolation of individual oocysts of different morphotypes, which had their genetic material extracted, amplified and sequenced in 4 non-overlapping loci in cox1, cox3 genes and fragments of small and large subunit rDNA of mitochondrial DNA. The sequences were 100% identical between the morphotypes, with the exception of a very small divergence observed at the locus that partially covers the cox3 gene. The phylogenetic analysis was inconclusive for the locus within cox1 gene traditionally used for eimeriid coccidians, however the other loci should have a promising future for phylogenetic studies when more sequences for the same genic regions are deposited in GenBank. Finally, the multifactorial analysis of the current work supported that the polymorphic oocysts shed from P. plumbea are a single species, which was named as Eimeria patagioenasae, making this the twenty-second eimerian description from Columbiformes.

Isospora coerebae Berto, Flausino, Luz, Ferreira and Lopes, 2010 Host: Bananaquit Coereba flaveola (Linnaeus, 1758) (Passeriformes: Thraupidae: Coerebinae) Locality: Private Natural Heritage Reserve of Porangaba (22°48’29.83’’S; 43°49’38.77’’W), Municipality of Itaguaí, State of Rio de Janeiro, Southeastern Brazil. Specimens: Photomicrographs are deposited and available (http://r1.ufrrj.br/labicoc/colecao.html) in the Parasitology Collection of the Laboratório de Biologia de Coccídios, at UFRRJ, under repository number 115/2021. Photovouchers of the host specimens are deposited in the same collection. Representative DNA sequence: DNA amplification of the COI1 and COI2 genic regions showed clear bands around ~250 bp and ~650 bp, respectively. Representative sequences were deposited in the GenBank database under the accession numbers: OK194671 (COI1); and OK194672 (COI2). Site of infection: Unknown. Prevalence: 100% (1/1). Sporulation: Exogenous. All oocysts were passed in the feces unsporulated and were fully sporulated by day 7 in K 2 Cr 2 O 7 solution at room temperature (20–25°C). Morphology (Fig 1 A-F): Oocyst (n = 15) sub-spherical, 22−27 × 22−25 (24.4 × 22.9); length/width (L/W) ratio 1.0−1.1 (1.07). Wall bi-layered, smooth outer wall about 2/3 total thickness, 1.5−1.8 (1.7). Micropyle, polar granules and oocyst residuum absent. Sporocyst elongate ovoidal, 16−19 × 10−11 (17.6 × 10.5); L/W ratio 1.6−1.8 (1.67). Stieda body present, prominent and rounded, 1.3−1.5 × 1.9−2.3 (1.4 × 2.1). Sub-Stieda body present, short and wide, 1.0−1.6 × 3.1−3.7 (1.3 × 3.5). Para-Stieda body absent. Crystalloid body sometimes present in the center of the sporocyst. Sporocyst residuum present, composed of many scattered granules of different sizes. Sporozoites vermiform, with a prominent posterior refractile body. Remarks: The oocysts recovered from the bananaquit in the current work are morphologically equivalent to the original description of I. coerebae, which have a typical and easily identifiable morphology, mainly in the Stieda and Sub-Stieda bodies (Berto et al. 2010). Table 1 compares the morphology and morphometry of the oocysts from the current work with those from the original description of I. coerebae. The typical morphology of the Stieda and Sub-Stieda bodies of I. coerebae are the basis of differentiation from another congeneric species, Isospora cagasebi Berto, Flausino, Luz, Ferreira and Lopes, 2008, which has the same host- and locality-types: C. flaveola in the Marambaia Island (Berto et al. 2008) (Table 1). However, in the current work no oocyst of I. cagasebi was observed, which, despite being similar in some characteristic features to I. coerebae, their sporocysts have knob-like Stieda body and prominent Sub-Stieda body, while I. coerebae has rounded Stieda body and short and wide Sub-Stieda body (Berto et al. 2010). Phylogenetic analysis: Phylogenetic analysis included sequences from coccidians available in GenBank (Figs. 2; 3). Toxoplasma gondii (Nicolle and Manceaux, 1908) was used as the outgroup. In the phylogenetic analysis based on the COI1 gene (Fig. 2), I. coerebae was recovered in a clade with the highest similarity of 99% with Isospora sepetibensis Berto, Flausino, Luz, Ferreira and Lopes, 2008 from black-goggled tanagers Trichothraupis melanops (Vieillot, 1818) of the Itatiaia National Park, which is located in the mountainous region in the interior of Southeastern Brazil. In the COI2 based phylogenetic analysis (Fig. 3), I. coerebae sat in a large clade with Isospora spp. from passerines of North America, Europe and Asia, with the highest similarity of 98% with Isospora greineri Hafeez, Stasiak, Delnatte, El-Sherry, Smith and Barta, 2014 from superb starlings Lamprotornis superbus Rüppell, 1845 at the Toronto Zoo, Toronto, Canada., Published as part of Ortúzar-Ferreira, Carlos Nei, Andrade, Lucas De Assis S., Genovezoliveira, Jhon Lennon, Oliveira, Mariana S., Mello, Ericson R., Cardozo, Sergian V., Oliveira, Águida A., Lima, Viviane M., Ferreira, Ildemar & Berto, Bruno P., 2022, Molecular identification of Isospora coerebae Berto, Flausino, Luz, Ferreira & Lopes, 2010 (Chromista: Miozoa: Eimeriidae) from the bananaquit Coereba flaveola (Linnaeus, 1758) (Passeriformes: Thraupidae: Coerebinae) from Brazil, pp. 83-91 in Zootaxa 5168 (1) on pages 85-86, DOI: 10.11646/zootaxa.5168.1.7, http://zenodo.org/record/6876923, {"references":["Berto, B. P., Flausino, W., Luz, H. R., Ferreira, I. & Lopes, C. W. G. (2010) Isospora coerebae n. sp. (Apicomplexa: Eimeriidae) from the bananaquit Coereba flaveola (Passeriformes: Coerebidae) in South America. Revista Brasileira de Parasitologia Veterinaria, 20 (1), 22 - 26. https: // doi. org / 10.1590 / S 1984 - 29612011000100005"]}

Ortúzar-Ferreira, Carlos Nei, Andrade, Lucas De Assis S., Genovezoliveira, Jhon Lennon, Oliveira, Mariana S., Mello, Ericson R., Cardozo, Sergian V., Oliveira, Águida A., Lima, Viviane M., Ferreira, Ildemar, Berto, Bruno P. (2022): Molecular identification of Isospora coerebae Berto, Flausino, Luz, Ferreira & Lopes, 2010 (Chromista: Miozoa: Eimeriidae) from the bananaquit Coereba flaveola (Linnaeus, 1758) (Passeriformes: Thraupidae: Coerebinae) from Brazil. Zootaxa 5168 (1): 83-91, DOI: https://doi.org/10.11646/zootaxa.5168.1.7

Isospora coerebae Berto, Flausino, Luz, Ferreira & Lopes, 2010 is a coccidian protozoan described from bananaquits Coereba flaveola (Linnaeus, 1758), on Marambaia Island, which is located on the southeastern Brazilian coast. In this current work, I. coerebae is identified from C. flaveola in a protected area close to Marambaia Island, but on the mainland, establishing a new location of parasitism, in addition to providing a preliminary genotypic characterization via sequencing of two regions of the mitochondrial cytochrome c oxidase subunit 1 (COI) gene. Its oocysts are sub-spherical, 24.4 × 22.9 μm, with smooth, bilayered wall, ~1.7 μm thick. Micropyle, polar granules and oocyst residuum are absent. Sporocysts are elongate ovoidal, 17.6 × 10.5 μm. Stieda body prominent and rounded and sub-Stieda body short and wide. Sporocyst residuum is composed of scattered granules of different sizes. Sporozoites are vermiform with a prominent posterior refractile body. The oocysts of the current work are morphologically equivalent to the original description of I. coerebae, which have a typical and easily identifiable morphology, mainly in the Stieda and Sub-Stieda bodies. The two sequenced gene regions of the COI gene approximated I. coerebae to Isospora spp. from Southeastern Brazil, but also from Isospora spp. from passerines of North America, Europe and Asia. Although there is a small overlap between the two genic regions sequenced in the current work, it is estimated that the longer COI sequence, which was recently designed and still not widespread, should show better phylogenetic results in the future.

Neste artigo pretende-se mostrar uma investigação que vem sendo feita em escolas de formação profissional brasileiras com o uso de Células de Inovação (CI). As CI's são grupos de estudantes envolvidos com a tarefa de solucionar um problema concreto da de sua escola ou bairro utilizando-se de conhecimentos técnico-científicos. Procurou-se investigar, através de uma metodologia etnográfica, como esses alunos aprendem os conteúdos de ciência ao solucionar seu problema e se a inovação é algo que possa ser aprendido. As situações trabalhados são problematizadas numa perspectiva CTS e, em seguida, são discutidas soluções. A implementação das melhores soluções gera nova discussão CTS sobre seu impacto. O trabalho aqui descrito cobre apenas uma célula. Pretende-se que a partir de 2010 que a investigação estenda-se para várias células trabalhando em rede.

Background: Osteoarthritis (OA) is considered the third most prevalent chronic non-communicable disease in the Brazilian population, being responsible for a high rate of physical disability and reduced quality of life. Little has been discussed about the social factors associated with this health condition. This study aimed to analyze the social factors associated with OA in the Brazilian population. Methods: This is a cross-sectional study based on data from the 2013 National Health Survey in Brazil with OA as its main outcome. Gender, age, body weight, usual activities, physical activity, self-perceived health and diagnosis of depression were analyzed as independent variables. Descriptive and inferential statistics were used. Poisson multiple regression was performed, and the prevalence ratio (PR) and confidence interval (CI) of 95% were calculated using a significance level of 5% (p≤0.05). Results: A total of 60,202 individuals of both genders took part in this study and the OA prevalence was 6.4%. The individual factors associated with a higher prevalence of OA were female gender (PR = 2.09; CI = 1.95-2.25), age over 35 years (PR = 2.88; CI = 2.57-3.24) and excess body weight (PR = 1.61; CI = 1.25-2.07). The presence of OA showed an association with lower performance of usual activities (PR = 1.61; CI = 1.50-1.73) and self-perceived health as very poor (PR = 3.96; CI = 3.31-4.72). In addition, it was associated with a higher prevalence of mental illnesses such as depression (PR = 1.77; CI = 1.64-1.90). Conclusion: Social and modifiable factors which are associated with a higher prevalence of osteoarthritis can be controlled through incentive measures such as social participation and physical activity.

Isospora brasilsatoae Oliveira and Berto n. sp. Type host: Yellow-legged thrush Turdus flavipes (Vieillot, 1818) (Aves: Passeriformes: Turdidae). Other hosts: Pale-breasted thrush Turdus leucomelas Vieillot, 1818 (Aves: Passeriformes: Turdidae); creamybellied thrush Turdus amaurochalinus Cabanis, 1850 (Aves: Passeriformes: Turdidae). Type locality: Parque Nacional do Itatiaia (22°26'57"S, 44°36'25"W), Southeastern Brazil. Other localities: Cacaria (22°42'51"S, 43°50'38"W) and Guapimirim (22°31'10"S, 43°00'36"W), also from Southeastern Brazil. Type-material: Photosyntypes, line drawing and oocysts recovered from T. flavipes in 2.5% K 2 Cr 2 O 7 solution (Williams et al. 2010) are deposited at the Museu de Zoologia at the Universidade Federal Rural do Rio de Janeiro, Brazil, under accession number MZURPTZ2018007. Photomicrographs are also deposited and available (http:// r1.ufrrj.br/labicoc/colecao.html) in the Parasitology Collection of the Laboratório de Biologia de Coccídios, at UFRRJ, under repository number P-90/2018. Photographs of the type-host specimen (symbiotype) are deposited in the same collection. ZooBank registration: To comply with the regulations set out in article 8.5 of the amended 2012 version of the International Code of Zoological Nomenclature (ICZN, 2012), details of the new species have been submitted to ZooBank. The Life Science Identifier (LSID) for Isospora brasilsatoae is urn:lsid:zoobank.org:act: 6C3FEE82- FC9C-4A58-8C17-1713532F03F3. Site of infection: Unknown. Prevalence: Four of 10 (40%). Sporulation: Exogenous. All oocysts were passed in the feces unsporulated and were fully sporulated by day 7 in K 2 Cr 2 O 7 solution at room temperature (20–25°C). Etymology: The specific name is derived from the family name of a Brazilian parasitologist Dr Marília de Carvalho Brasil Sato, given in her honor for her contribution to the study of taxonomy and ecology of parasites. Description (Figs 1; 2 A–C). Oocyst (n = 15) ovoidal to pyriform, 25-30 × 19-23 (26.8 × 21.1); length/width (L/W) ratio 1.2-1.4 (1.27). Wall bi-layered, delicate, 1.4-1.8 (1.6) thick, outer layer smooth, c. 2/3 of total thickness. Micropyle present, without micropyle cap, wrinkles or invagination of the in inner layer perceptible. Oocyst residuum absent, but 1 to 3 rounded polar granules are present. Sporocyst elongate ovoidal with tapered anterior end (where it is Stieda/sub- Stieda complex), 17-19 × 10-11 (18.1 × 10.5); L/W ratio 1.6-1.9 (1.72). Stieda body present, knob-like, 2.0 high × 2.0 wide. Sub-Stieda body present, trapezoidal, 1.5 high × 3.5 wide. Para-Stieda body absent. Sporocyst residuum present, as a distinctly sub-spherical body consisting of numerous small granules that appear to be membranebounded, 6–9 (7.5). Sporozoites vermiform, with anterior and posterior refractile bodies and striations. Remarks: Isospora brasilsatoae is different from other coccidian species of Turdidae of the New World on several aspects (Table 1). It is the only one to have a pyriform shape of the oocysts. It has a micropyle, which is a characteristic present only in the oocysts of Isospora albicollis Lainson and Shaw, 1989, Isospora sabiai Pinho, Rodrigues, Silva, Lopes, Oliveira, Ferreira, Cardozo, Luz, Ferreira, Lopes and Berto, 2017 and Isospora machadoae Pinho, Silva, Rodrigues, Lopes, Oliveira, Luz, Ferreira, Lopes and Berto, 2018 (Lainson & Shaw 1989; Pinho et al. 2017a; 2017b; 2018). From these three, but also from the others, I. brasilsatoae can be easily differentiated by having up to three rounded polar granules, sporocysts elongate ovoidal (L/W ratio> 1.6), sub- Stieda body trapezoidal, and compact residuum.

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Texto completo: acesso restrito. p.2420-2427 Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2013-07-30T16:01:34Z No. of bitstreams: 1 2420.full.pdf: 509563 bytes, checksum: 7a3488e2bfca5bb34d604293b619acf2 (MD5) Approved for entry into archive by Flávia Ferreira(flaviaccf@yahoo.com.br) on 2013-08-05T13:04:18Z (GMT) No. of bitstreams: 1 2420.full.pdf: 509563 bytes, checksum: 7a3488e2bfca5bb34d604293b619acf2 (MD5) Made available in DSpace on 2013-08-05T13:04:18Z (GMT). No. of bitstreams: 1 2420.full.pdf: 509563 bytes, checksum: 7a3488e2bfca5bb34d604293b619acf2 (MD5) Previous issue date: 2010 Saint Louis encephalitis virus (SLEV), a member of the genus Flavivirus (family Flaviviridae), is an encephalitogenic arbovirus broadly distributed in the Americas. Phylogenetic analysis based on the full-length E gene sequences obtained for 30 Brazilian SLEV strains was performed using different methods including Bayesian and relaxed molecular clock approaches. A new genetic lineage was suggested, hereafter named genotype VIII, which co-circulates with the previously described genotype V in the Brazilian Amazon region. Genotypes II and III were restricted to São Paulo state (South-east Atlantic rainforest ecosystem). The analysis also suggested the emergence of an SLEV common ancestor between 1875 and 1973 (mean of 107 years ago), giving rise to two major genetic groups: genotype II, more prevalent in the North America, and a second group comprising the other genotypes (I and III–VIII), broadly dispersed throughout the Americas, suggesting that SLEV initially emerged in South America and spread to North America. In conclusion, the current study demonstrates the high genetic variability of SLEV and its geographical dispersion in Brazil and other New World countries.

Dealing with work groups is a challenge for organizations where projects are the main focus. During their activities if cooperation is not adequately coordinated and well supported it can provoke significant delays on the project or can put the final product quality at risk. The goal of this paper is to describe the risks control module, named Risys, to support the execution of project developed by the use of Web-based Groupware on Project Management System (WGPMS). This infrastructure is comprised of groupware applications which are based both on computational intelligence and Monte Carlo technique. Experiments which were already carried out reveal valuable results related to the implemented rules on Risys. However, additional case studies need to be carried out from the moment that new applications were integrated on WGPMS, as well as new rules that were added to the database system.

Este trabalho é parte de uma pesquisa que teve como objetivo investigar como o conhecimento científico presente no tema cinética química foi sendo apropriado pelos livros didáticos de Química de ensino médio. Investigaram-se as concepções sobre ciência vinculadas à abordagem deste assunto nos livros. Foram utilizadas categorias que representam as perspectivas filosóficas empiristas/indutivistas e a racionalista, tendo sido analisados 20 livros, no período de 1929 a 2004. Foi considerado o conteúdo referente aos modelos teóricos utilizados para explicar a velocidade das reações e a influência da concentração e da temperatura sobre essa. A perspectiva predominante nos livros foi a empirista/indutivista, independentemente da época em que foram editados, o que pode revelar que os autores não consideraram as mudanças de perspectiva que ocorreram durante esse período.