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Background The relationship between obesity and type 2 diabetes mellitus (T2DM) is inevitable. The increase in the occurrence of obesity all around the globe has proportionally increased the occurrence of comorbidities. Periodontitis is a multifactorial inflammation of the periodontium attributed to dysbiosis in the subgingival microflora which may ultimately result in tooth loss. A triad between T2DM, periodontitis, and obesity is ascertained. Aim The present study focuses on investigating the association of obesity and T2DM with periodontal health. Methodology This cross-sectional study was conducted for a period of six months (September 2022 to February 2023) on 181 subjects, as per the sample size calculated by the statistician, who were previously diagnosed with T2DM and were either obese or overweight. The glycemic control was assessed on the basis of HbA1c values of the subjects. The subjects underwent bioelectrical impedance analysis along with an anthropometric examination. Full mouth examination including bleeding on probing, pocket probing depth, clinical attachment level (CAL), and oral hygiene index-simplified (OHI-S) was also checked to assess the status of periodontal health, and periodontitis was classified according to the new classification of 2017. Results The obtained data was statistically analyzed and p-value≤0.05 was considered statistically significant. The maximum prevalence of Stage III Grade C Periodontitis (34.73%) was observed in the diabetic obese group than in the diabetic overweight group. The overall anthropometric variable, abdominal circumference, waist-hip ratio, and basal metabolic index (BMI) were higher in the obese group as they displayed poor glycemic control. BMI and CAL also showed a positive correlation. Conclusion A significant association between obesity and T2DM with periodontitis was confirmed by this study. Hence, a syndemic approach needs to be formulated by the medical fraternity in collaboration with dental surgeons for the effective management of this triad., Competing Interests: Human subjects: Consent was obtained or waived by all participants in this study. Institutional Ethics Committee, Lady Hardinge Medical College and Associated Hospitals, New Delhi issued approval LHMC/IEC/2022/PG Thesis/48. Animal subjects: All authors have confirmed that this study did not involve animal subjects or tissue. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work., (Copyright © 2024, Asthana et al.)

Background: Dental plaque may be attributed as a precursor to various oral health problems like dental caries, periodontal disease, halitosis, etc. With an ever-increasing awareness about the adverse effects of chemical formulations, emphasis is now being laid on the usage of herbal ingredients, as they are safer for long-term use in addition to their medicinal benefits., Aim: The present study aims to assess the anti-plaque and anti-inflammatory efficacy of herbal toothpaste compared to synthetic toothpaste among 20-40-year-old patients in Bareilly, Uttar Pradesh., Methodology: In this study, 130 subjects aged between 20 and 40 years with poor oral hygiene status and signs of gingival inflammation corresponding with chronic marginal gingivitis were selected. Subjects in Group 1 were prescribed herbal toothpaste (KUDOS Ayurveda), whereas patients in Group 2 were administered non-herbal toothpaste. Clinical parameters of gingival health specifically gingival index (GI), patient hygiene performance (PHP), and approximate plaque index (API) were recorded at baseline, 14 days, 28 days, and 42 days. Chi-square test, student-independent t-test, and paired t-test were performed to find significant differences in various variables between the two groups at different follow-up visits., Results: At the end of the study, a significant reduction in GI and API scores was obtained, along with increased PHP scores. The p-value was set at 0.05, and the power of the study was set at 0.95. There was a statistically significant reduction (p < 0.05) in GI and API and an improvement in PHP scores at various time intervals in Group 1 as compared to Group 2.  Conclusion: Regular application of herbal extract dentifrices for 42 days provided a significant reduction of dental plaque and improvement in overall gingival health without any adverse effects. This instilled motivation in the patients to uphold proper oral hygiene. Hence, herbal dentifrices could be prescribed as an adjunct to periodontal therapy in the maintenance phase., Competing Interests: Human subjects: Consent was obtained or waived by all participants in this study. Institute of Dental Sciences issued approval IEC/131/2021. Animal subjects: All authors have confirmed that this study did not involve animal subjects or tissue. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work., (Copyright © 2024, KK et al.)

Introduction: This study aimed to evaluate and compare postoperative pain (PP) in single-visit nonsurgical endodontic retreatment (SV NSER) with 2% chlorhexidine (CHX), 0.1% octenidine (OCT) with or without ozone, and 5.25% sodium hypochlorite (NaOCl)., Materials and Methods: In this randomized, parallel, prospective, double-blind, clinical trial, 132 single-rooted, root-filled teeth with symptomatic apical periodontitis (AP) requiring NSER were allocated into six groups randomly (n = 22/group): 2% CHX with NaOCl (CHXH), 2% Ozonated CHX without NaOCl (OCHX), 2% Ozonated CHX with NaOCl (OCHXH), 0.1% OCT with NaOCl (OCTH), 0.1% Ozonated OCT without NaOCl (OOCT), and 0.1% Ozonated OCT with NaOCl (OOCTH). Standard NSER protocol was followed groups were irrigated with 15 ml of ozonated or nonozonated irrigant (CHX/OCT) for 3-5 min with ultrasonic agitation. PP at baseline, after 6, 12, 24, 48 h, and 7 days was recorded using the Visual Analog Scale (VAS). Logistic regression of predictor variables was compared using the Chi-square test. For group-wise and time-wise comparisons, a two-way analysis of variance followed by the post hoc Bonferroni test was carried out., Results: None of the patient-related variables in logistic regression obtained a statistically significant (P > 0.05) role in PP. The VAS score after 6 h was OCHX (4.72) > OOCT (4.42) > CHXH (4.23) > OCTH (3.95) > OCHXH (3.42) > OOCTH (3.21). OOCTH and OCHXH groups demonstrated statistically significant reductions in VAS scores at various time intervals (P < 0.05)., Conclusion: SV NSER with ozonated OCT, CHX irrigation, and NaOCl resulted in lesser PP at all time intervals, i.e., 6, 12, 24, 48 h, and 7 days in patients with symptomatic AP., Competing Interests: There are no conflicts of interest., (Copyright: © 2024 Journal of Conservative Dentistry and Endodontics.)

Aim: The aim of the study was to compare the esthetic treatment outcome and quantification of tooth color changes using microabrasion and resin infiltration techniques of fluorotic white spot lesions (WSLs)., Subjects and Methods: Sixty-six teeth with fluorotic small opaque white areas involving 25%-50% (very mild/mild fluorosis) of the surface were randomly assigned into two groups for microabrasion and resin infiltration techniques. To quantify tooth color changes, depicted by Delta E (DE), photographic analysis was performed using Adobe Photoshop CS5 Extended version by measuring Commission Internationale de l'Eclairage L*a*b* values of each tooth at two points, i.e. one at WSL and the other one at sound adjacent enamel., Statistical Analysis Used: Data were analyzed with t-test using SPSS software version 23., Results: L*value (decrease in whiteness) of posttreatment WSL decreased in both groups but was higher in the resin infiltration group, which was statistically significant. There were no statistically significant changes observed in a* and b* values of WSL in both groups. DE value difference of pre and postoperative was higher in the resin infiltration group, which was statistically significant which indicated the stability of color obtained by the resin infiltration group., Conclusions: Resin infiltration technique is more efficient in the immediate elimination of fluorotic WSL than microabrasion., Competing Interests: There are no conflicts of interest., (Copyright: © 2023 Journal of Conservative Dentistry and Endodontics.)

Aim: This study evaluated the effect of three collagen cross-linking agents - proanthocyanidins (grape seed extract [GSE] and green tea extract [GTE]) and glutaraldehyde [GA] on microshear bond strength (μSBS) of caries-affected dentin (CAD)-resin complex., Materials and Methods: Freshly extracted 96 teeth with caries up to the middle third of dentin were sectioned through the deepest part of the occlusal fissure, perpendicular to the long axis of the crown. Caries was excavated with large round bur until firm dentin was obtained, confirmed by visual inspection and tactile examination. Flat occlusal dentin surfaces were treated as follows: Group-1 - 6.5% GSE ( n = 30), Group-2 - 2% GTE ( n = 30), Group-3 - 5% Glutaraldehyde ( n = 30), Group-4 - control group (no agents) ( n = 6). Each group was further divided into Subgroup A - Etch-N-Rinse 15s, Subgroup B - Etch-N-Rinse 45s, and Subgroup C - Self-etch. Two increments of 1.5-mm thick composite (Tetric-N-Ceram - Ivoclar Vivadent) with a 1-mm diameter were built-up. Each sample was subjected to μSBS test in Universal Testing Machine. Student's t -test was done for intragroup comparison and one-way ANOVA for intergroup comparison., Results: Statistically significant difference was present in mean μSBS, with Group 1B showing the best results and Group 4C, the least., Conclusions: Thus, the application of these collagen cross-linkers, to CAD, increases μSBS and promises a new approach to improve dentin bond strength., Competing Interests: There are no conflicts of interest., (Copyright: © 2023 Journal of Conservative Dentistry.)

Aim: This study was undertaken with an objective to find out the accuracy and reliability of presurgical ridge mapping (RM) on a diagnostic cast for linear measurements in the horizontal direction on cone-beam computed tomography (CBCT) and direct surgical assessment., Materials and Methods: In total, 25 patients based on inclusion and exclusion criteria were selected. An acrylic stent with two points marked at 3 mm and 6 mm from the soft tissue summit of the alveolar ridge was fabricated. Linear measurements at these points were estimated with three techniques viz. RM on the diagnostic cast, CBCT, and direct surgical in situ measurements after flap reflection., Results: Considering direct in situ surgical measurements as the gold standard with an accuracy of 100%, the accuracy for CBCT and RM on the diagnostic cast was 95.5% and 87.4%, respectively, for the maxillary arch. Whereas the accuracy for CBCT and RM on the diagnostic cast was 88.6% and 92.2%, respectively, for mandibular arch., Conclusion: The three approaches discussed are reliable for the assessment of ridge dimensions in the horizontal direction. Ridge mapping on the diagnostic cast is a simple, precise, noninvasive technique without any radiographic exposure., Clinical Significance: Ridge mapping on a diagnostic cast along with two-dimensional (2D) radiography can be used as a valuable chairside diagnostic tool in the treatment planning prior to dental implant placement in the cases of a regular alveolar ridge and even mucosal lining. This clinical technique provides zero radiation exposure and is also cost-effective.

Introduction: The aim of this study was to compare the effect of different application techniques of ozone on the prevalence of postendodontic pain in patients undergoing single-visit root canal treatment., Methods: hundred eight patients with necrotic pulp in single-rooted teeth and apical periodontitis participated in the trial. A standard single-visit endodontics protocol was followed with 5.25% sodium hypochlorite and rotary nickel-titanium files. After shaping and cleaning, patients were randomly allocated into the following groups: group 1 (n = 21), ozone treatment with no activation (NA); group 2 (n = 22), ozone treatment with manual dynamic activation (MDA); group 3, (n = 21), ozone treatment with passive ultrasonic activation (PUA); group 4 (n = 23), ozone treatment with sonic activation (SA); and group 5 (n = 21), no ozone treatment (the control group). Patient levels of discomfort were recorded at 6 different time intervals using the visual analog scale (VAS). Comparison of the mean difference between the groups and time intervals was performed using 2-way analysis of variance followed by a post hoc Bonferroni test. The level of significance was set at 5%., Results: VAS scores were highest for the control > NA > MDA > SA > PUA groups. A statistically significant reduction in VAS scores was observed in the PUA and SA groups in comparison with the NA, control, and MDA groups. Timewise comparison showed a highly significant decline in VAS scores at all time intervals (P < .001)., Conclusions: Ultrasonic and sonic activation of ozone resulted in less pain in patients undergoing single-visit endodontics compared with no ozone treatment., (Copyright © 2021 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.)

Background: The success of direct pulp capping (DPC) depends on the preoperative assessment of pulpal status, intraoperative judgment after pulp exposure, and the biomaterials used to cap the pulp., Aim: The study aims to compare the clinical and radiographic responses of the pulp-dentin complex after DPC with TheraCal LC, Biodentine, and current gold standard mineral trioxide aggregate (MTA) Plus., Materials and Methods: Ninety vital permanent teeth with Class I deep carious lesions were randomly divided into three different groups. After the caries excavation, hemostasis was established using sodium hypochlorite at the site of pulp exposure on which the material was placed. Clinical and radiographic follow-ups were performed at 1-, 3-, and 6-month intervals., Results: Overall success rates of MTA Plus, Biodentine, and TheraCal LC were statistically insignificant., Conclusions: TheraCal LC and Biodentine showed similar success rates when compared to MTA Plus and can be used as an agent in DPC., Competing Interests: There are no conflicts of interest., (Copyright: © 2021 Journal of Conservative Dentistry.)

Aim and Objectives: The aim of the study was to investigate the effect of two different collagen cross-linking agents proanthocyanidin (Grape seed extract [GSE] and 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide) on the surface topography of etched dentin and microtensile bond strength (μTBS) of resin dentin bond., Materials and Methods: Fifty-two sound human 3 rd molars were collected, and their occlusal surfaces were ground flat to expose dentin. Dentin surfaces were etched using phosphoric acid and then teeth were randomly divided into four groups, according to the dentin treatment: Group 1: wet bonding technique, Group 2: dry bonding technique, Group 3: 6.5% proanthocyanidin, and Group 4: 0.1M carbodiimide. Scanning electron microscope analysis was done for twenty specimens ( n = 5 per group) at ×10,000 and ×30,000 magnification. Remaining 32 specimens were restored with TETRIC N-Bond adhesive systems and resin composite. After 24 h, teeth were sectioned to produce a cross-sectional surface area of 1.0 mm 2 and tested for μTBS., Statistical Analysis: Data were statistically analyzed using ANOVA and post hoc least significant difference test ( P < 0.05)., Conclusion: When acid-etched dentin is treated by 6.5% proanthocyanidin (GSE) and 0.1M carbodiimide, followed by application of adhesives, it results in increased μTBS due to cross-linking of collagen fibrils., Competing Interests: There are no conflicts of interest., (Copyright: © 2021 Journal of Conservative Dentistry.)

Objective: This study aimed to evaluate the thickness of oxygen inhibition layer (OIL), produced on various composite materials, and to compare their interlayer shear bond strength (SBS), by treating the OIL with various agents., Materials and Methods: The thickness of OIL of three different composite materials (120 specimens divided into three groups) Group 1 - Ivoclar Tetric N-Ceram (nanohybrid composite), Group 2 - Ivoclar Te Econom Plus (microhybrid composite), and Group 3 - GC EverX Posterior (a short glass fiber-reinforced composite) was evaluated. Each group was divided into four subgroups (A, B, C, and D) depending on the surface treatment given - no surface treatment (control group), ethanol, water spray, and grinding with SiC paper. This was followed by interlayer SBS testing., Statistical Analysis: The data were statistically analyzed using ANOVA at a significance level of P < 0.05. Tukey's post hoc analysis was performed following ANOVA to determine differences among the groups., Results: The control group showed higher SBS irrespective of the type of composite material. The group treated with SiC paper resulted in the lowest interlayer SBS among all groups. Glass fiber composite showed higher interlayer SBS compared to both nanohybrid and microhybrid composites, irrespective of the surface treatment given., Conclusion: The OIL, which acts as an intermediate layer, is retained on the surface of the composite even after treatment with ethanol and water spray. The presence of an OIL improved the interlayer SBS of two adjacent composite layers and led to more durable adhesion. Rather, the absence of an OIL adversely affected the bond strength and led to adhesive interfacial failures., Competing Interests: There are no conflicts of interest., (Copyright: © 2020 Journal of Conservative Dentistry.)

Creation of genetic variability and development of varieties having higher yield potential depends on information about nature of gene action. The present investigation was undertaken to decipher the nature of gene action and allied genetic parameters involved in the inheritance of yield and yield-related component traits in opium poppy ( Papaver somniferum L.). The biparental inbreeding progenies derived from four segregating base populations of crosses NB-1Kr40-3/3×NB-1Kr30+0.2-2/1, NB-5Kr40-7/2×58/1, NB-1Kr30+0.2-2/1×58/1 and NB-Kr40-3/3×NB-5Kr40-7/2 of opium poppy were analysed to study the gene actions involved in the inheritance of yield and component traits. Additive component of variance played a predominant role in North Carolina design (NCD)-I, while both additive and dominance genetic components were found important in NCD-III design. The presence of additive as well as nonadditive components of variance suggested that one or two generations of intermating in further generations followed by selection may lead to development of novel genotypes.

Objective: The aim of this study was to evaluate defects caused by torsional fatigue in used hand and rotary nickel-titanium (Ni-Ti) instruments by stereomicroscopic examination., Materials and Methods: One hundred five greater taper Ni-Ti instruments were used including Protaper universal hand (Dentsply Maillefer, Ballaigues, Switzerland), Protaper universal rotary (Dentsply Maillefer, Ballaigues, Switzerland), and Revo-S rotary (MicroMega, Besançon, France) files. Files were used on lower anterior teeth. After every use, the files were observed with both naked eyes and stereomicroscope at 20× magnification (Olympus, Shinjuku, Tokyo, Japan) to evaluate defects caused by torsional fatigue. Scoring was assigned to each file according to the degree of damage., Statistics: The results were statistically analyzed using the Mann-Whitney U test and the Kruskal-Wallis test., Results: A greater number of defects were seen under the stereomicroscope than on examining with naked eyes. However, the difference in methods of evaluation was not statistically significant. Revo-S files showed minimum defects, while Protaper universal hand showed maximum defects. The intergroup comparison of defects showed that the bend in Protaper universal hand instruments was statistically significant., Conclusion: Visible defects in Ni-Ti files due to torsional fatigue were seen by naked eyes as well as by stereomicroscope. This study emphasizes that all the files should be observed before and after every instrument cycle to minimize the risk of separation.

The present study aimed to investigate the delivery potential of Etodolac (ETD) containing topical niosomal gel. Niosomal formulations were prepared by thin film hydration method at various ratios of cholesterol and Span 60 and were evaluated with respect to particle size, shape, entrapment efficiency, and in vitro characteristics. Dicetyl phosphate (DCP) was also added in the niosomal formulation. Mean particle size of niosomal formulation was found to be in the range of 2 μm to 4 μm. Niosomal formulation N2 (1 : 1) ratio of cholesterol and surfactant displayed good entrapment efficiency (96.72%). TEM analyses showed that niosomal formulation was spherical in shape. Niosomal formulation (N2) displayed high percentage of drug release after 24 h (94.91) at (1 : 1) ratio of cholesterol : surfactant. Further selected niosomal formulation was used to formulate topical gel and was characterized with respect to its various parameters such as pH, viscosity, spreadability, ex vivo study, and in vivo potential permeation. Ex vivo study showed that niosomal gel possessed better skin permeation study than the plain topical gel. Further in vivo study revealed good inhibition of inflammation in case of topical niosomal gel than plain gel and niosomal formulation. The present study suggested that topical niosomal gel formulations provide sustained and prolonged delivery of drug.

The present study was focused on formulating and evaluating clarithromycin (CLR) containing niosomal formulation for in vitro and in vivo pharmacokinetic behavior. Niosomal formulations (empty and drug loaded) were prepared by using different ratio of surfactant (various Span grades 20, 40, 60, and 80) and cholesterol by thin film hydration method and were evaluated for in vitro characteristics, stability studies, and in vivo study. Dicetyl phosphate (DCP) was added to the niosomal formulation. Various pharmacokinetic parameters were determined from plasma of male SD rats. Span 60 containing niosomal formulation NC2 (cholesterol to surfactant ratio 1 : 1) displayed highest entrapment efficiency with desired particle size of 4.67 μm. TEM analyses showed that niosomal formulation was spherical in shape. Niosomes containing Span 60 displayed higher percentage of drug release after 24 h as compared to other formulations. NC2 formulation was found to be stable at the end of the study on storage condition. Various pharmacokinetic parameters, namely, AUC, AUMC, and MRT of niosomal formulation, were found to be 1.5-fold, 4-fold, and 3-fold plain drug, respectively. The present study suggested that niosomal formulations provide sustained and prolonged delivery of drug with enhance bioavailability.

The purpose of the present study was to formulate polymeric self-emulsifying curcumin nanocapsules with high encapsulation efficiency, good emulsification ability, and optimal globule size for localized targeting in the colon. Formulations were prepared using modified quasiemulsion solvent diffusion method. Concentration of formulation variables, namely, X1 (oil), X2 (polymeric emulsifier), and X3 (adsorbent), was optimized by design of experiments using Box-Behnken design, for its impact on mean globule size (Y1) and encapsulation efficiency (Y2) of the formulation. Polymeric nanocapsules with an average diameter of 100-180 nm and an encapsulation efficiency of 64.85±0.12% were obtained. In vitro studies revealed that formulations released the drug after 5 h lag time corresponding to the time to reach the colonic region. Pronounced localized action was inferred from the plasma concentration profile (C max 200 ng/mL) that depicts limited systemic absorption. Roentgenography study confirms the localized presence of carrier (0-2 h in upper GIT; 2-4 h in small intestine; and 4-24 h in the lower intestine). Optimized formulation showed significantly higher cytotoxicity (IC50 value 20.32 μM) in HT 29 colonic cancer cell line. The present study demonstrates systematic development of polymeric self-emulsifying nanocapsule formulation of curcumin for localized targeting in colon.

The investigation was undertaken to investigate the formation and association of neural network and their fibres with nerve cells and ganglia in the parenchyma. The staining of the neural network was done by the cholinesterase technique under the maintained pH 5.2, temperature 40 degrees C and incubation period 30 hrs. The neural network was formed by a good number of tortuous thick nerves and was related either with nerve cells or ganglia. At times, it was formed by a good number of thick nerves and a few thin nerves in the connective tissue space.