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Figure S2. Identification in vitro of mutants altered in trichome development. a Most type I trichomes of the mutant 744-P73 have abnormal terminal cells. b The leaves of 1491-MM have fewer type I trichomes. c The leaves of 912-P73 have fewer trichomes of types I and VI. d The leaves (right, up) and stem (right, down) of the mutant 4728-SP have a greater number of trichomes with respect to Solanum pennellii wild-type plants (left, up and down). Bar = 1 mm. (PPTX 2580 kb)

Figure S6. Flowering time in plants of the mutant Altered in all reproductive traits (Art). a. Number of phytomers up to the first inflorescence (0–1) and between inflorescences (1–2, 2–3, …). b Length from base up to the first inflorescence (0–1) and between inflorescences (1–2, 2–3, …). (PPTX 63 kb)

Table S1. Phenotype inheritance in tomato mutants altered in leaf development. (DOCX 20 kb)

Table S3. Co-segregation analysis between phenotype and a T-DNA insert with a functional nptII gene in mutants altered in leaf development. (DOCX 14 kb)

Figure S5. Vegetative and reproductive development of the tomato mutant necrotic and small leaflets (nsl). a. Mutant nsl seedlings (right) develop smaller leaves than WT (left). b. In the mutant nsl the leaves of shoot apex-derived plants have small necrotic spots (right), which does not occur in the WT (left). c. In nsl greenhouse-grown plants adult leaves have a great number of small leaflets that exhibit necrotic spots in the leaf blade. d. In nsl mutant plants a decrease in quantitative parameters related to the number of fruits per inflorescence, fruit set rate and fruit size is observed. Bar = 1 cm. (PPTX 627 kb)

Figure S1. Morphology of the leaves of greenhouse-grown plants of tomato cv ‘P73’ and some dominant mutants. a Fifth leaf of a tomato plant cv. ‘P73’. b-c The mutants 816-P73 (b) and 740-P73 (c) have darker, less lobed leaves. d-f The mutants 136-P73 (d), 605-P73 (e) and 14-P73 (f) develop leaflets of different morphology. g The mutant 630-P73 develops small leaves with irregular arrangement of leaflets. h The mutant 860-P73 has more complex leaves with smaller leaflets. Bar = 1 cm. (PPTX 922 kb)

Table S2. Number of T-DNA inserts with a functional nptII marker gene in tomato mutants altered in leaf development. (DOCX 19 kb)

Figure S3. Vegetative and reproductive development of the tomato mutant wiry-like-150 (wl-150). a Basal leaves of wl-150 have a certain degree of leaf blade expansion, while the following exhibit shoestring shape. b The inflorescence of wl-150 (right) is more branched than that of wild-type ‘P73’ plants (left). Note that the inflorescences of wl-150 also alternate vegetative and reproductive traits. c Flowers of wl-150 have thread-like sepals and petals as well as an open anther cone. d The mutant wl-150 is partially fertile since it develops fruits ranging from small seedless (right, up) to others of normal size with some seeds (right, down) similar to that of wild-type ‘P73’ (fruit on the left). Bar = 1 cm. (PPTX 492 kb)

Tomato CALCINEURIN B-LIKE PROTEIN 10 (SlCBL10) ensures plant growth by regulating proper distribution of Na+ and Ca2+ in the shoot apical meristem and developing organs under salt stress.