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2. Draft genome sequence of Bradyrhizobium sp. strain BR 3262, an effective microsymbiont recommended for cowpea inoculation in Brazil

Author

Simões-Araújo, Jean Luiz, Leite, Jakson, Marie Rouws, Luc Felicianus, Passos, Samuel Ribeiro, Xavier, Gustavo Ribeiro, Rumjanek, Norma Gouvêa, and Zilli, Jerri Édson

Subjects
Next generation sequencing, food and beverages, Atlantic forest area, Biological nitrogen fixation, Nodulation
Abstract

The strain BR 3262 was isolated from nodule of cowpea (Vigna unguiculata L. Walp) growing in soil of the Atlantic Forest area in Brazil and it is reported as an efficient nitrogen fixing bacterium associated to cowpea. Firstly, this strain was assigned as Bradyrhizobium elkanii, however, recently a more detailed genetic and molecular characterization has indicated it could be a Bradyrhizobium pachyrhizi species. We report here the draft genome sequence of B. pachyrhizi strain BR 3262, an elite bacterium used as inoculant for cowpea. The whole genome with 116 scaffolds, 8,965,178 bp and 63.8% of C+G content for BR 3262 was obtained using Illumina MiSeq sequencing technology. Annotation was added by the RAST prokaryotic genome annotation service and shown 8369 coding sequences, 52 RNAs genes, classified in 504 subsystems.

Published
2016

3. Draft genome sequence of Bradyrhizobium sp. strain BR 3262, an effective microsymbiont recommended for cowpea inoculation in Brazil.

Author

Simões-Araújo JL, Leite J, Marie Rouws LF, Passos SR, Xavier GR, Rumjanek NG, and Zilli JÉ

Subjects
Base Composition, Brazil, Genes, Bacterial, Nitrogen Fixation, Open Reading Frames, Root Nodules, Plant microbiology, Bradyrhizobium classification, Bradyrhizobium genetics, Genome, Bacterial, Genomics methods, Symbiosis, Vigna microbiology, Vigna physiology
Abstract

The strain BR 3262 was isolated from nodule of cowpea (Vigna unguiculata L. Walp) growing in soil of the Atlantic Forest area in Brazil and it is reported as an efficient nitrogen fixing bacterium associated to cowpea. Firstly, this strain was assigned as Bradyrhizobium elkanii, however, recently a more detailed genetic and molecular characterization has indicated it could be a Bradyrhizobium pachyrhizi species. We report here the draft genome sequence of B. pachyrhizi strain BR 3262, an elite bacterium used as inoculant for cowpea. The whole genome with 116 scaffolds, 8,965,178bp and 63.8% of C+G content for BR 3262 was obtained using Illumina MiSeq sequencing technology. Annotation was added by the RAST prokaryotic genome annotation service and shown 8369 coding sequences, 52 RNAs genes, classified in 504 subsystems., (Published by Elsevier Editora Ltda.)

Published
2016
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4. Draft genome sequence of Bradyrhizobium sp. strain BR 3262, an effective microsymbiont recommended for cowpea inoculation in Brazil

Author

Norma Gouvêa Rumjanek, Jean Luiz Simões-Araújo, Jerri Édson Zilli, Jakson Leite, Gustavo Ribeiro Xavier, Samuel Ribeiro Passos, and Luc Felicianus Marie Rouws

Subjects
0301 basic medicine, Short Communication, lcsh:QR1-502, Nodulation, medicine.disease_cause, Microbiology, Bradyrhizobium, Genome, DNA sequencing, lcsh:Microbiology, Vigna, Open Reading Frames, 03 medical and health sciences, Nitrogen Fixation, Next generation sequencing, Botany, medicine, Atlantic forest area, Symbiosis, Bradyrhizobium elkanii, Whole genome sequencing, Base Composition, biology, food and beverages, Genomics, Genome project, Biological nitrogen fixation, biology.organism_classification, 030104 developmental biology, Genes, Bacterial, Root Nodules, Plant, Brazil, Genome, Bacterial, Bradyrhizobium pachyrhizi
Abstract

The strain BR 3262 was isolated from nodule of cowpea (Vigna unguiculata L. Walp) growing in soil of the Atlantic Forest area in Brazil and it is reported as an efficient nitrogen fixing bacterium associated to cowpea. Firstly, this strain was assigned as Bradyrhizobium elkanii, however, recently a more detailed genetic and molecular characterization has indicated it could be a Bradyrhizobium pachyrhizi species. We report here the draft genome sequence of B. pachyrhizi strain BR 3262, an elite bacterium used as inoculant for cowpea. The whole genome with 116 scaffolds, 8,965,178 bp and 63.8% of C+G content for BR 3262 was obtained using Illumina MiSeq sequencing technology. Annotation was added by the RAST prokaryotic genome annotation service and shown 8369 coding sequences, 52 RNAs genes, classified in 504 subsystems.

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