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12. Linear dsDNA Partitions Spontaneously into the Inverse Hexagonal Lyotropic Liquid Crystalline Phases of Phospholipids

Author

Black, Camilla F., Wilson, Richard J., Nylander, Tommy, Dymond, Marcus K., and Attard, George S.

Abstract

Recently, we reported that DNA associated with inverse hexagonal (HII) lyotropic liquid crystal phases of the lipid 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) was actively transcribed by T7 RNA polymerase.(1) Our findings suggested that key components of the transcription process, probably the T7 RNA polymerase and the DNA, remained associated with the monolithic HIIphase throughout transcription. Here, we investigate the partitioning of DNA between an HIIlyotropic liquid crystal phase and an isotropic supernatant phase in order to develop insights into the localization of DNA in liquid crystalline environments. Our results show that linear double stranded DNA (dsDNA) molecules partition spontaneously into monolithic preformed HIIliquid crystal phases of DOPE. We propose that this process is driven by the increase in entropy due to the release of counterions from the DNA when it inserts into the aqueous pores of the HIIphase.

Published
2024
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18. Linear dsDNA partitions spontaneously into the inverse hexagonal lyotropic liquid crystalline phases of phospholipids

Author

Black, Camilla F., Wilson, Richard J., Nylander, Tommy, Dymond, Marcus K., and Attard, George S.

Subjects
Phospholipids -- Research, DNA -- Research, Liquid crystals -- Research, Chemistry
Abstract

The partitioning of DNA between an [H.sub.II] lyotropic liquid crystal phase and an isotropic supernatant phase is explored to gain better insights into the localization of DNA in liquid crystalline environments. The spontaneous partition of linear double stranded DNA (dsDNA) molecules into monolithic preformed [H.sub.II] liquid crystal phases of the lipid 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) is observed to be driven by the increase in entropy due to the release of counterions from the DNA when it inserts into the aqueous pores of the [H.sub.II] phase.

Published
2010

20. Periodic binary Si:Ti, Si:Al mixed macroporous oxides with ultra-high heteroatom loading: a facile sol-gel approach

Author

Hant, Steven M., Attard, George S., Riddle, Rodney, Ryan, Kevin M., EPSRC, Industrial Host Fellowship, Merck Chemicals Ltd., and European 5th Framework

Subjects
inverse opal, aluminium, sol-gel, titanium, mixed oxide, colloidal crystal templating
Abstract

peer-reviewed Highly ordered macroporous mixed metal oxides were prepared with very high mixed metal ratios using an optimized silicon alkoxide prehydrolysis process. The homogenous solutions completely filled the interstitial voids in polymethylmethacrylate (PMMA) artificial opal templates. Subsequent template removal resulted in highly ordered aluminosilicate and titanosilicate inverse opal pore networks. The optimized process allowed the fabrication of periodic binary metal oxide frameworks with 2:1 Si:Al loadings and 1:1 Si:Ti loadings. The mixed metal oxides did not show any phase segregation during high temperature template removal as evidenced by X-ray diffraction (XRD), energy dispersive X-ray analysis (EDX) and Fourier transform infrared spectroscopy (FT-IR). The as formed macroporous metal oxides demonstrate excellent substrate adherence and mechanical stability and showed refractive index modulation in direct relation to the silicon/heterometal ratio in the precursor sol.

Published
2005

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