Type 1 Diabetes Mellitus is one of the most frequent chronic diseases where its incidence increased from birth to 12 years with a peak between 10-14 years of age. Insulin therapy is a drug of choice and become a routine part of daily life for diabetic children /families. However, errors in injection technique and self-dosing increase the likelihood of serious adverse events as lipohypertrophy, hypo and hyperglycemia that can be avoided through increase mothers and diabetic children awareness Study aim was evaluate effect of an Evidence Based Insulin Injection Guidelines Application on Mothers' Awareness about insulin injection and its complication on their diabetic children. Methodology: A quasi experimental design was used Setting: inpatient department and outpatient clinic of endocrinology. Subjects was 61 mothers of school age diabetic children. Four tools were used; I was an interview questionnaire that assessed sociodemographic characteristics of mothers and also children plus children clinical data ;II was insulin Injection Technique Questionnaire (ITQ) that assessed mothers' insulin injection knowledge ,attitude and skills ;III was insulin injection checklist that assessed mothers' pen and syringe insulin injection techniques and tool IV lipohypertrophy checklist that assessed presence and grades of diabetic children lipohypertrophy. Results: regarding total percentage score of mothers Knowledge, Attitude and Practices about insulin injection pre, immediately and post 6 months from Evidence-Based Insulin Injection Guidelines Application were improved with statistically significant differences p=Keywords: T1DM= Type 1 Diabetes Mellitus, IDDM= Insulin Dependent Diabetes Mellitus, EBIIGA= Evidence-Based Insulin Injection Guidelines Application, KAP = Knowledge, Attitude and Practices, LH= lipohypertrophy Title: Effectiveness of an Evidence Based Insulin Injection Guidelines Application on Diabetic Children Mothers' Awareness about insulin injection: A quasi experimental study Author: Hoda Mohamed Nafee, Eman Abd Elsamea Elguoshy, Hoda Ghareeb, Rehab Abd El Aziz El Sayed Abd El Aziz International Journal of Novel Research in Healthcare and Nursing ISSN 2394-7330 Vol. 9, Issue 1, January 2022 - April 2022 Page No: 353-366 Novelty Journals (Publisher) Website: www.noveltyjournals.com Published date: 30-April-2022 DOI: https://doi.org/10.5281/zenodo.6508334 Paper download link (Source): https://www.noveltyjournals.com/upload/paper/Effectiveness%20of%20an%20Evidence-30042022-1.pdf, Novelty Journals (Publisher), Website: www.noveltyjournals.com, International Journal of Novel Research in Healthcare and Nursing, ISSN 2394-7330, {"references":["[1]\tkkooorns, C., Dunn, A., Brady, M., Starr, N., Blosser, C., Garzon, D. 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