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32 results on '"Au, Sam H."'

9. Clusters of circulating tumor cells traverse capillary-sized vessels

Author

Au, Sam H., Storey, Brian D., Moore, John C., Tang, Qin, Chen, Yeng-Long, Javaid, Sarah, Sarioglu, A. Fatih, Sullivan, Ryan, Madden, Marissa W., O’Keefe, Ryan, Haber, Daniel A., Maheswaran, Shyamala, Langenau, David M., Stott, Shannon L., and Toner, Mehmet

Published
2016

18. Effect of Ice Nucleation and Cryoprotectants during High Subzero-Preservation in Endothelialized Microchannels

Author

Tessier, Shannon N., primary, Weng, Lindong, additional, Moyo, Will D., additional, Au, Sam H., additional, Wong, Keith H. K., additional, Angpraseuth, Cindy, additional, Stoddard, Amy E., additional, Lu, Chenyue, additional, Nieman, Linda T., additional, Sandlin, Rebecca D., additional, Uygun, Korkut, additional, Stott, Shannon L., additional, and Toner, Mehmet, additional

Published
2018
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26. Hydrogel discs for digital microfluidics

Author

Fiddes, Lindsey K., primary, Luk, Vivienne N., additional, Au, Sam H., additional, Ng, Alphonsus H. C., additional, Luk, Victoria, additional, Kumacheva, Eugenia, additional, and Wheeler, Aaron R., additional

Published
2012
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30. Microfluidic Isolation of Circulating Tumor Cell Clusters by Size and Asymmetry

Author

Au, Sam H., Edd, Jon, Stoddard, Amy E., Wong, Keith H. K., Fachin, Fabio, Maheswaran, Shyamala, Haber, Daniel A., Stott, Shannon L., Kapur, Ravi, and Toner, Mehmet

Abstract

Circulating tumor cell clusters (CTC clusters) are potent initiators of metastasis and potentially useful clinical markers for patients with cancer. Although there are numerous devices developed to isolate individual circulating tumor cells from blood, these devices are ineffective at capturing CTC clusters, incapable of separating clusters from single cells and/or cause cluster damage or dissociation during processing. The only device currently able to specifically isolate CTC clusters from single CTCs and blood cells relies on the batch immobilization of clusters onto micropillars which necessitates long residence times and causes damage to clusters during release. Here, we present a two-stage continuous microfluidic chip that isolates and recovers viable CTC clusters from blood. This approach uses deterministic lateral displacement to sort clusters by capitalizing on two geometric properties: size and asymmetry. Cultured breast cancer CTC clusters containing between 2–100 + cells were recovered from whole blood using this integrated two-stage device with minimal cluster dissociation, 99% recovery of large clusters, cell viabilities over 87% and greater than five-log depletion of red blood cells. This continuous-flow cluster chip will enable further studies examining CTC clusters in research and clinical applications.

Published
2017
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31. Circulating tumor cells shed large extracellular vesicles in capillary-sized bifurcations.

Author

Vrynas A, Arfan S, Satia K, Bazban-Shotorbani S, Ashna M, Zhang A, Visan D, Chen A, Carter M, Blackhall F, Simpson KL, Dive C, Huang P, and Au SH

Abstract

Circulating tumor cells (CTCs) and their clusters are the drivers of metastasis, but their interactions with capillary beds are poorly understood. Using microfluidic models mimicking human capillary bifurcations, we observed cell size- and bifurcation-dependent shedding of nuclei-free fragments by patient CTCs, CTC-derived explant cells and numerous cancer cell lines. Shedding reduced cell sizes up to 61%, facilitating their transit through bifurcations. We demonstrated that shed fragments were a novel class of large extracellular vesicles (LEVs), whose proteome was associated with immune-related and signaling pathways. LEVs were internalized by endothelial and immune cells, disrupted endothelial barrier integrity and polarized monocytes into M2 tumor-promoting macrophages. Cumulatively, these findings suggest that CTCs shed LEVs in capillary beds that drive key processes involved in the formation of pre-metastatic niches., Competing Interests: Competing interests: None to declare.

Published
2024
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32. Circulating Tumor Cell Cluster Sorting by Size and Asymmetry.

Author

Au SH

Subjects
Humans, Cell Separation methods, Cell Line, Tumor, Microfluidics methods, Cell Movement, Neoplastic Cells, Circulating pathology, Microfluidic Analytical Techniques methods
Abstract

Circulating tumor cell (CTC) clusters are rare yet potent initiators of metastasis and may be useful as clinical biomarkers. Numerous techniques have been developed to isolate individual circulating tumor cells from the blood, but these techniques are often ineffective at capturing CTC clusters and may cause cluster damage or dissociation during processing or recovery. This chapter describes methods for fabricating and operating a two-stage continuous microfluidic chip that isolates and recovers viable CTC clusters from blood or biological fluids using deterministic lateral displacement., (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)

Published
2023
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