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3. B04 Minipig models of Huntington’s disease at pigmod center

Author

Runa Vochozková, Petra, primary, Auch, Hannah, additional, Nemesh, Kateryna, additional, Duong Nyguen, The, additional, Sekáč, David, additional, Kamenná, Eva, additional, Baxa, Monika, additional, Klíma, Jiri, additional, Ardan, Taras, additional, Juhás, Stefan, additional, Levinská, Božena, additional, Juhásová, Jana, additional, Keller, Jiri, additional, Klempir, Jiri, additional, Motlik, Jan, additional, Klymiuk, Nikolai, additional, and Ellederová, Zdenka, additional

Published
2022
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4. Early disruption of photoreceptor cell architecture and loss of vision in a humanized pig model of usher syndromes

Author

Grotz, Sophia, Schäfer, Jessica, Wunderlich, Kirsten A, Ellederova, Zdenka, Auch, Hannah, Bähr, Andrea, Runa‐Vochozkova, Petra; https://orcid.org/0000-0003-3691-0340, Fadl, Janet, Arnold, Vanessa, Ardan, Taras, Veith, Miroslav; https://orcid.org/0000-0001-6254-436X, Santamaria, Gianluca, Dhom, Georg, Hitzl, Wolfgang; https://orcid.org/0000-0002-7696-1479, Kessler, Barbara; https://orcid.org/0000-0001-7036-238X, Eckardt, Christian, Klein, Joshua, Brymova, Anna, Linnert, Joshua, Kurome, Mayuko, Zakharchenko, Valeri, Fischer, Andrea, Blutke, Andreas, Döring, Anna, Suchankova, Stepanka, Popelar, Jiri, Rodríguez‐Bocanegra, Eduardo, Dlugaiczyk, Julia; https://orcid.org/0000-0001-7693-9127, Straka, Hans, May‐Simera, Helen; https://orcid.org/0000-0002-9477-4036, et al, Grotz, Sophia, Schäfer, Jessica, Wunderlich, Kirsten A, Ellederova, Zdenka, Auch, Hannah, Bähr, Andrea, Runa‐Vochozkova, Petra; https://orcid.org/0000-0003-3691-0340, Fadl, Janet, Arnold, Vanessa, Ardan, Taras, Veith, Miroslav; https://orcid.org/0000-0001-6254-436X, Santamaria, Gianluca, Dhom, Georg, Hitzl, Wolfgang; https://orcid.org/0000-0002-7696-1479, Kessler, Barbara; https://orcid.org/0000-0001-7036-238X, Eckardt, Christian, Klein, Joshua, Brymova, Anna, Linnert, Joshua, Kurome, Mayuko, Zakharchenko, Valeri, Fischer, Andrea, Blutke, Andreas, Döring, Anna, Suchankova, Stepanka, Popelar, Jiri, Rodríguez‐Bocanegra, Eduardo, Dlugaiczyk, Julia; https://orcid.org/0000-0001-7693-9127, Straka, Hans, May‐Simera, Helen; https://orcid.org/0000-0002-9477-4036, and et al

Abstract

Usher syndrome (USH) is the most common form of monogenic deaf-blindness. Loss of vision is untreatable and there are no suitable animal models for testing therapeutic strategies of the ocular constituent of USH, so far. By introducing a human mutation into the harmonin-encoding USH1C gene in pigs, we generated the first translational animal model for USH type 1 with characteristic hearing defect, vestibular dysfunction, and visual impairment. Changes in photoreceptor architecture, quantitative motion analysis, and electroretinography were characteristics of the reduced retinal virtue in USH1C pigs. Fibroblasts from USH1C pigs or USH1C patients showed significantly elongated primary cilia, confirming USH as a true and general ciliopathy. Primary cells also proved their capacity for assessing the therapeutic potential of CRISPR/Cas-mediated gene repair or gene therapy in vitro. AAV-based delivery of harmonin into the eye of USH1C pigs indicated therapeutic efficacy in vivo.

Published
2022

5. Early disruption of photoreceptor cell architecture and loss of vision in a humanized pig model of usher syndromes

Author

Grotz, Sophia, primary, Schäfer, Jessica, additional, Wunderlich, Kirsten A, additional, Ellederova, Zdenka, additional, Auch, Hannah, additional, Bähr, Andrea, additional, Runa‐Vochozkova, Petra, additional, Fadl, Janet, additional, Arnold, Vanessa, additional, Ardan, Taras, additional, Veith, Miroslav, additional, Santamaria, Gianluca, additional, Dhom, Georg, additional, Hitzl, Wolfgang, additional, Kessler, Barbara, additional, Eckardt, Christian, additional, Klein, Joshua, additional, Brymova, Anna, additional, Linnert, Joshua, additional, Kurome, Mayuko, additional, Zakharchenko, Valeri, additional, Fischer, Andrea, additional, Blutke, Andreas, additional, Döring, Anna, additional, Suchankova, Stepanka, additional, Popelar, Jiri, additional, Rodríguez‐Bocanegra, Eduardo, additional, Dlugaiczyk, Julia, additional, Straka, Hans, additional, May‐Simera, Helen, additional, Wang, Weiwei, additional, Laugwitz, Karl‐Ludwig, additional, Vandenberghe, Luk H, additional, Wolf, Eckhard, additional, Nagel‐Wolfrum, Kerstin, additional, Peters, Tobias, additional, Motlik, Jan, additional, Fischer, M Dominik, additional, Wolfrum, Uwe, additional, and Klymiuk, Nikolai, additional

Published
2022
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6. Early Disruption of Photoreceptor Cell Architecture and Loss of Vision in a Humanized Pig Model of Usher Syndrome

Author

Grotz, Sophia, primary, Schäfer, Jessica, additional, Wunderlich, Kirsten A., additional, Ellederova, Zdenka, additional, Auch, Hannah, additional, Bähr, Andrea, additional, Runa-Vochozkova, Petra, additional, Plutniok, Janet, additional, Arnold, Vanessa, additional, Ardan, Taras, additional, Veith, Miroslav, additional, Santamaria, Gianluca, additional, Dhom, Georg, additional, Hitzl, Wolfgang, additional, Kessler, Barbara, additional, Kurome, Mayuko, additional, Zakharchenko, Valeri, additional, Linnert, Joshua, additional, Fischer, Andrea, additional, Blutke, Andreas, additional, Döring, Anna, additional, Suchankova, Stepanka, additional, Popelar, Jiri, additional, May-Simera, Helen, additional, Laugwitz, Karl-Ludwig, additional, Vandenberghe, Luk H., additional, Wolf, Eckhard, additional, Nagel-Wolfrum, Kerstin, additional, Motlik, Jan, additional, Fischer, M. Dominik, additional, Wolfrum, Uwe, additional, and Klymiuk, Nikolai, additional

Published
2021
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7. Modifying Bacterial Artificial Chromosomes for Extended Genome Modification.

Author

Auch H, Klymiuk N, and Runa-Vochozkova P

Subjects
Animals, Chromosomes, Artificial, Bacterial genetics, Mammals genetics, Transfection, Genome, Genomics
Abstract

Bacterial artificial chromosomes have been used extensively for the exploration of mammalian genomes. Although novel approaches made their initial function expendable, the available BAC libraries are a precious source for life science. Their comprising of extended genomic regions provides an ideal basis for creating a large targeting vector. Here, we describe the identification of suitable BACs from their libraries and their verification prior to manipulation. Further, protocols for modifying BAC, confirming the desired modification and the preparation of transfection into mammalian cells are given., (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)

Published
2022
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