1. Detection of anti-IFI16 antibodies by ELISA: clinical and serological associations in systemic sclerosis
- Author
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Pier Luigi Meroni, Gian Domenico Sebastiani, Paolo Airò, Mirko Scarsi, Valeria Caneparo, Valeria Riccieri, Santo Landolfo, Renato Alberto Sinico, Marisa Gariglio, Roberto Gerli, Silvia Costa, Angela Tincani, Milvia Lotzniker, Gabriella Morozzi, Antonella Afeltra, Paola Faggioli, Antonella Radice, Francesca Bellisai, Michele Mondini, Costa, S, Mondini, M, Caneparo, V, Afeltra, A, Airò, P, Bellisai, F, Faggioli, P, Gerli, R, Lotzniker, M, Meroni, P, Morozzi, G, Radice, A, Riccieri, V, Scarsi, M, Sebastiani, G, Sinico, R, Tincani, A, Gariglio, M, and Landolfo, S
- Subjects
Male ,Systemic disease ,Autoantibodies, Diagnosis, IFI16, Interferon, Systemic sclerosis ,Serology ,Systemic sclerosi ,Immunopathology ,Diagnosis ,Medicine ,Pharmacology (medical) ,skin and connective tissue diseases ,Nuclear Protein ,education.field_of_study ,integumentary system ,biology ,Nuclear Proteins ,Middle Aged ,Autoantibodie ,Connective tissue disease ,Antibodies, Anti-Idiotypic ,RNA, Bacterial ,DNA Topoisomerases, Type I ,Phosphoprotein ,Antibodies, Antinuclear ,Interferon ,Systemic sclerosis ,Female ,Antibody ,Case-Control Studie ,Diagnosi ,Human ,Population ,Enzyme-Linked Immunosorbent Assay ,Rheumatology ,Humans ,Clinical significance ,IFI16 ,education ,Autoantibodies ,Aged ,Scleroderma, Systemic ,business.industry ,autoantibodies ,diagnosis ,ifi16 ,interferon ,systemic sclerosis ,Autoantibody ,Biomarker ,medicine.disease ,Phosphoproteins ,Case-Control Studies ,Immunology ,biology.protein ,business ,Biomarkers - Abstract
Objectives: To validate the clinical significance of anti-IFI16 autoantibodies in SSc and assess their associations with serological markers of SSc. Methods: A semi-quantitative ELISA was used to detect anti-IFI16 autoantibodies in the sera of 344 SSc patients from seven Italian hospitals and 144 healthy controls. SSc-associated autoantibodies [anti-RNA polymerase III (anti-RNAP III) antibodies, anti-centromere, anti-topo I] and IF patterns were evaluated using commercial assays. Statistical analyses were performed to test clinical and serological associations. Results: The results of this study confirm a significant prevalence (29%) of anti-IFI16 antibodies in the SSc population (n = 344). Anti-IFI16 antibodies were also detected in 30% of the SSc patients who tested negative for both ACAs and anti-topo I (anti-Scl70) antibodies. In this subgroup of patients, anti-IFI16 antibodies were significantly associated with the limited cutaneous form of SSc with a sensitivity of 40% and a specificity of 81%. Moreover, analysis of the distribution of anti-RNAP III antibodies vs anti-IFI16 in the same SSc population showed that they were mutually exclusive. IIF revealed no association between anti-IFI16 and fluoroscopic patterns, due to a lack of IFI16 autoantigen in HEp-2 cells. Anti-IFI16 antibody levels were also significantly associated with heart involvement. Conclusions: Anti-IFI16 autoantibodies are frequently detected in SSc, displaying clinical and laboratory associations, and being particularly useful for diagnosis and disease classification in patients who are negative for other SSc serological markers. © The Author 2010. Published by Oxford University Press on behalf of the British Society for Rheumatology. All rights reserved.
- Published
- 2011