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10. VITAMINE IDROSOLUBILI

Author

Avigliano, L, Cairella, G, Garbagnati, F, Taruscio, D, Ruggeri, S, Savini, I, and Simonetti, P

Subjects
nutrienti, assunzioni raccomandate, assunzioni raccomandate, Settore MED/49 - Scienze Tecniche Dietetiche Applicate, Settore BIO/10, nutrienti
Published
2014

11. Induction of gene expression via activator protein-1 in the ascorbate protection against UV-induced damage

Author

Catani, M V, Rossi, A, Costanzo, A, Sabatini, S, Levrero, M, Melino, G, and Avigliano, L

Subjects
Keratinocytes, Ultraviolet Rays, Gene Expression Profiling, vitamin c, Radiation-Protective Agents, Ascorbic Acid, Cell Biology, Radiation Tolerance, Biochemistry, Transcription Factor AP-1, fra-1, Gene Expression Regulation, Humans, Mitogen-Activated Protein Kinase 8, Mitogen-Activated Protein Kinases, Settore BIO/10, jnk, Molecular Biology, Research Article, Signal Transduction
Abstract

UV irradiation is a major insult to the skin. We have shown previously that exogenous vitamin C (ascorbate) accumulates in HaCaT keratinocytes, thus conferring the ability to prevent radical formation and cell death elicited by UV-B. Here, we have investigated the potential mechanisms accounting for the cytoprotective effects exerted by this antioxidant. Using a cDNA microarray hybridization, we identified several genes whose expression was up-regulated by ascorbate. We focused on the fra-1 gene, a member of the Fos family of transcription factors that down-regulates activator protein-1 (AP-1) target genes. Both in HaCaT and in normal human epidermal keratinocytes, we found Fra-1 mRNA induction as early as 2h after ascorbate loading. Electrophoretic mobility-shift assay and antibody supershift analysis revealed that ascorbate modulates AP-1 DNA-binding activity and that Fra-1 is in AP-1 complexes in treated cells. Furthermore, transient-transfection studies, using an AP-1 reporter construct, showed that ascorbate was able to inhibit both basal and UV-B-induced AP-1-dependent transcription. Ascorbate also modulates UV-B-induced AP-1 activity by preventing the phosphorylation and activation of the upstream c-Jun N-terminal kinase (JNK), thus inhibiting phosphorylation of the endogenous c-Jun protein. These data suggest that ascorbate mediates cellular responses aimed at counteracting UV-mediated cell damage and cell death by interfering at multiple levels with the activity of the JNK/AP-1 pathway and modulating the expression of AP-1-regulated genes.

Published
2001
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28. Human platelets express authentic CB₁ and CB₂ receptors

Author

Catani, M. V, Gasperi, V, Catanzaro, Giuseppina, Baldassarri, S, Bertoni, A, Sinigaglia, F, Avigliano, L, and Maccarrone, M.

Subjects
Adult, Blood Platelets, Male, Gene Expression, Enzyme-Linked Immunosorbent Assay, Inositol 1,4,5-Trisphosphate, Tritium, Binding, Competitive, Receptor, Cannabinoid, CB2, Young Adult, Piperidines, Receptor, Cannabinoid, CB1, Cyclic AMP, Humans, Drug Interactions, Settore BIO/10, Camphanes, Platelet Count, Integrin beta3, Cyclohexanols, Flow Cytometry, Pyrazoles, Calcium, Female, Bornanes, Protein Binding, Rimonabant
Abstract

In the last decade, the neurovascular effects exerted by endocannabinoids (eCBs) have attracted growing interest, because they hold the promise to open new avenues of therapeutic intervention against major causes of death in Western society. Several actions of eCBs are mediated by type-1 (CB₁) or type-2 (CB₂) cannabinoid receptors, yet there is no clear evidence of the presence of these proteins in platelets. To demonstrate that CB₁ and CB₂ are expressed in human platelets, we analyzed their protein level by Western blotting and ELISA, visualized their cellular localization by confocal microscopy, and ascertained their functionality by binding assays. We found that CB₁, and to a lesser extent CB₂, are expressed in highly purified human platelets. Both receptor subtypes were predominantly localized inside the cell, thus explaining why they might remain undetected in preparations of plasma membranes. The identification of authentic CB₁ and CB₂ in human platelets supports the potential exploitation of selective agonists or antagonists of these receptors as novel therapeutics to combat neurovascular disorders. It seems remarkable that some of these substances have been already used in humans to treat disease states.

Published
2010

29. Vitamin C in human platelets

Author

Savini, I, Avigliano, L, and Catani, Mv

Subjects
Settore MED/49 - Scienze Tecniche Dietetiche Applicate
Published
2010

32. Ecto-NOX1 expression in human platelets

Author

Avigliano, L, Arnone, R, Rossi, A, Catani, Mv, DEL PRINCIPE, D, and Savini, I

Subjects
Settore BIO/11, Settore MED/49 - Scienze Tecniche Dietetiche Applicate, Settore BIO/10
Published
2009

36. Endocannabinoids prolong platelet life span

Author

Avigliano, L, Catani, Mv, Gasperi, V, FINAZZI AGRO', A, and Maccarrone, M

Subjects
Settore BIO/10, Settore MED/49 - Scienze Tecniche Dietetiche Applicate
Published
2008

40. Catalytic and spectroscopic properties of cytochrome-c, horseradish peroxidase and ascorbate oxidase embedded in a sol-gel silica matrix as a function of gelation time

Author

Savini, I, Santucci, R, DI VENERE, A, Rosato, N, Strukul, G, Pinna, F, and Avigliano, L

Subjects
spectroscopy, Protein Denaturation, Time Factors, Protein Conformation, Cytochrome c Group, Lycaeninae, Catalysis, Fluorescence, cytochrome c oxidase, enzyme mechanism, Rosales, Settore BIO/10, enzyme analysis, Horseradish Peroxidase, nonhuman, Spectrometry, Circular Dichroism, Armoracia rusticana, article, Silicon Dioxide, reaction analysis, Kinetics, Ascorbate Oxidase, Cucumis sativus, cucumber, oxidation reduction reaction, Oxidation-Reduction, ascorbate oxidase, horseradish peroxidase, circular dichroism, enzyme modification, Spectrometry, Fluorescence
Published
1999

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