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Nowadays, nanoparticle-based cancer therapy plays a prominent role in the medicine. In present work, two nanoliposomes LIP-PEG-FA-FITC-Fe3O4-Cis (N-Cis) and LIP-PEG-FA-FITC-Fe3O4-Gem (N-Gem) were synthesized and characterized by analytical devices such as FT-IR, DLS, TEM, and SEM. The size, drug entrapment efficiency and drug release behavior of liposomal derivatives were investigated. The cytotoxicity, apoptosis, cellular uptake, and gene expression analysis were carried out on A549, MCF-7, and MRC5 cell lines. The IC50 values of the nanocomplexes exhibit cytotoxic effects more than pure drugs (P-Cis and P-Gem) and also, N-Cis reveals more cytotoxicity as compared to N-Gem on studied cell lines, significantly. While N-Gem showed somewhat better apoptosis-inducing performance than N-Cis sample by Flow cytometry analysis. The results of cellular uptake display that folate receptor-targeted liposomal nano-complexes are able to improve cellular uptake in MCF-7 than A549 cancer cells. N-Cis and N-Gem liposomal nano-complexes increased the expression of P21 and P53 genes in cancer cells more than that of P-Cis and P-Gem. Also, up regulation of mentioned genes expression using N-Cis is more than N-Gem. Ultimately, these data are depicting that our targeted nano-complexes can be considered as a promising useful and effective delivery system in active cancer therapy.

In this research, we reported the preparation and properties of lipid-loaded nanoparticle modified with polyethylene glycol (PEG) and folic acid (FA) as a co-drug delivery system for controlling the release of cisplatin (Cis) and gemcitabine (Gem). Then, LIP-PEG-FA-Cis-Gem nano-complex was characterized by FT-IR, DLS, TEM, SEM, and XRD. The results suggest that these nano-complexes have spherical morphology with appropriate size and surface charge. The drug entrapment efficiencies were calculated about 22.96% (Cis) and 25% (Gem). In vitro drug release profile and then the therapeutic efficiency of LIP-PEG-FA-Cis-Gem nano-complex was also investigated on A549, MCF-7 and normal MRC5 cell lines. The cell viability value was observed 10.39 ± 4.88% (A549), 7.56 ± 4.87% (MCF7), and 23.2 ± 6.29% (MRC5) after 48 h of treatment with 8 μM concentration. Additionally, apoptosis amounts have indicated 83.0 ± 0.70% and 82.5 ± 0.21% for A549 and MCF7 cell lines after 48 h posttreatment, respectively. Moreover, the apoptotic P21 and P53 gene expression were observed 13.85 ± 0.73 and 17.16 ± 2.12 folds for A549, 6.78 ± 0.84 and 13.08 ± 0.84 folds for MCF7 after 48 h of incubation with LIP-PEG-FA-Cis-Gem IC50 concentrations, respectively. Therefore, our synthetic nano-liposome can be an excellent candidate for co-delivery of Gem & Cis agents to folat receptor-expressing tumors and the inhibition of cancer cells.

Metal nanoparticles are one option for targeted drug delivery. In order to increase antibiotic efficiency and decrease its side effects, antibiotic conjugated nanoparticles have been known as a suitable approach. The aim of this study was optimization of gold nanoparticle biosynthesis byEscherichia coliDH5α and its conjugation with gentamicin. For this purpose gold nanoparticles were biosynthesized from HAuCl4and confirmed by Uv/ Vis, XRD, DLS and SEM. Then the effects of different parameters on optimum conditions for gold nanoparticles production were investigated. The MIC and MBC of gentamicin and its conjugate were investigated againstE. coli,Clostridium perfringensandClostridium botulinum. The results revealed that among different treatments, centrifuge (10000 rpm, 10 min) and sonication are the optimum conditions for gold nanoparticle production with less than 10 nm sizes. Filtration was also the best method for purifying nanoparticles. The conjugated nanoparticles significantly reduced the MIC of gentamicin againstE. coliand also overcame the natural resistance of tested anaerobic bacteria. In conclusion, the optimized method is an effective, inexpensive and environmental friendly method for biosynthesis of gold nanoparticles. Overcoming natural resistance of anaerobic bacteria using antibiotic conjugates with nanoparticles provides hopes for further experiments and in vivo studies.