41 results on '"Aziz Bouymajane"'
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2. Enhancing and hindering biodegradation: A comparative study on polyamide 6 reinforced with bio-fillers (peanut shell, olive pomace, and plaster)
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Oumayma Oulidi, Ibtissam Elaaraj, Maria Jabri, Asmae Nakkabi, Aziz Bouymajane, Fouzia Rhazi Filali, Mohammed Fahim, and Noureddine El Moualij
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Polyamide 6 ,Peanut shell ,Olive pomace ,Plaster ,Biodegradability ,Alcaligenes faecalis ,Chemistry ,QD1-999 ,Environmental technology. Sanitary engineering ,TD1-1066 - Abstract
In recent years, bio-reinforced composite materials have occupied an important class among the materials of mass use in our daily lives thanks to their potential advantages such as lightness, low cost, ease of implementation, and in particular biodegradation. The latter has been the objective of several studies focusing particularly on poorly biodegradable polymers, among these polymers we find polyamide 6 (PA6). It is a semi-crystalline polymer, distinguished by its good mechanical properties, excellent chemical and thermal stability, and low price compared to other polyamides. In this research, we investigated the impact of reinforcing PA6 with peanut shell powder (PSP), olive pomace powder (OPP), and plaster (PL) on its biodegradation process. Our objective was to determine whether the incorporation of these reinforcements accelerates, delays, or has no effect on PA6 biodegradation. To achieve this, we conducted degradation experiments using the bacterium Alcaligenes faecalis (AF), isolated from the public landfill of Meknes, Morocco. Our findings revealed intriguing insights into the biodegradation behavior of the PA6 composites. Specifically, the incorporation of PSP significantly enhanced PA6 biodegradation, resulting in a notable 38 % weight loss. Conversely, the inclusion of OPP led to a delay in biodegradation, with only a 19 % weight loss observed. Interestingly, the addition of plaster exhibited a biodegradation pattern similar to that of raw PA6. Furthermore, we utilized FTIR and SEM coupled with EDS elemental analysis to confirm the biodegradation of the composites. These techniques provided valuable insights into the structural, morphological, and elemental changes undergone by the composites during the biodegradation process. Overall, our study contributes novel insights into the biodegradation dynamics of PA6 composites reinforced with natural materials. By elucidating the effects of reinforcement on biodegradation, our research paves the way for the development of sustainable and eco-friendly composite materials.
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- 2024
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3. Juniperus oxycedrus leaves and berries extracts: HPLC-PDA-ESI/MS2 phenolic characterization and in vitro anti-inflammatory effects
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Mohamed Reda Kachmar, Yassine Oulad El Majdoub, Andreia P. Oliveira, Aziz Bouymajane, Hanae Naceiri Mrabti, Toufik Bouddine, Nora Mir, Nidal Naceiri Mrabti, Hajji Lhoussain, Zoubida Haloui, Alessia Tropea, Francesco Cacciola, Luigi Mondello, Paula B. Andrade, and Said Chakir
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Juniperus oxycedrus ,Polyphenols ,Food ,Liquid chromatography ,Anti-inflammatory ,Other systems of medicine ,RZ201-999 - Abstract
Background: Inflammation is a natural response of the body to tissue injury or infection, but chronic inflammation can contribute to the development of various diseases. Therefore, finding effective ways to reduce inflammation is of great interest to researchers and healthcare providers. The aim of the study was the elucidation of the phenolic profile from J. oxycedrus leaves and berries followed by in vitro antioxidant and anti-inflammatory assays. Despite the existence of some works on this species, most of these researchers focus their attention on cade oil and only few were interested to study leaves and berries extracts. Methods: HPLC-DAD-ESI/MS2 was used for their characterization of the phenolic profile whereas spectrophotometric microassays in non-cellular and cellular systems (RAW 264.7 macrophages cell line) was assessed for their in vitro antioxidant and anti-inflammatory potentials. Results: Up to twenty-one polyphenolic compounds were positively identified in both extracts. Both leaves and berries extracts showed strong antioxidant capacity to scavenge O2●, with IC50 values of 22.86 ± 2.40 and 122.62 ± 9.42 µg/mL. Moreover, both revealed to have potential to inhibit 5-lipoxygenase in a dose-dependent way, being leaves the most effective vegetal material. Conclusion: The results suggest that J. oxycedrus leaves and berries could be considered exquisite sources of bioactive molecules for a potential further application in the food and pharmaceutical industries.
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- 2024
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4. Chemical composition and antibacterial activity of essential oil of Pelargonium graveolens and its fractions
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Zakya M'hamdi, Aziz Bouymajane, Ouassima Riffi, Fouzia Rhazi Filali, Mostafa Ettarchouch, Mohammed ELhourri, and Ali Amechrouq
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Pelargonium graveolens ,Essential oil ,Fractions ,GC-MS ,Antibacterial activity ,Chemistry ,QD1-999 - Abstract
This work aims to study the chemical composition and evaluate the antibacterial activity of Pelargonium graveolens essential oil and its fractions collected in the Er-Rachidia region of Morocco. GC-MS analysis of Pelargonium graveolens essential oil and its fractions yielded majority compounds such as; epi-γ-Eudesmol (16.67 %), Geraniol (12.54 %), β-Citronellol (12, 34 %), Citronellyl formate (7.70 %) and Geranyl tiglate (5.21 %), for the crude essential oil of Pelargonium graveolons, while the fractions that is obtained by preparative plate chromatography gave the following compounds, fraction 1 consists mainly of β-Citronellol (35. 83 %) Geraniol (38.78 %), fraction 2 is dominated by epi-γ-Eudesmol (55. 10 %) and α-agorofuran (8.41 %)), as well as fraction 3 is revealed the presence of Geranylgeraniol (23.70 %) and epi-γ-Eudesmol (17.53 %) and Phenylethyl tiglate (12.01 %), fraction 4 consists mainly of Phenylethyl tiglate (58.19 %) and α-agorofuran (8.49 %), then the fraction 5 is represented by the compound majorities such as Geranyl tiglate (30.75 %) and Geranyl butanoate (10.94 %), while the fraction 6 is characterized mainly by 1-Isopropyl-4,7-dimethyl-1,2,3,5,6,8a-hexahydronaphthalene (12.08 %). Concerning the antibacterial activity of the essential oil and its fractions 1, 2, 3, and 4 showed bactericidal power against all tested bacteria: Listeria monocytogenes, Escherichia coli, Staphylococcus aureus, and Salmonella typhimurium, also fractions 5 and 6 have bactericidal power against Escherichia coli, Salmonella typhimurium and no power against Staphylococcus aureus and Listeria monocytogenes.
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- 2024
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5. Phenolic Compound, Antioxidant, Antibacterial, and In Silico Studies of Extracts from the Aerial Parts of Lactuca saligna L.
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Aziz Bouymajane, Fouzia Rhazi Filali, Soumia Moujane, Yassine Oulad El Majdoub, Philipp Otzen, Souhail Channaoui, Abdelaziz Ed-Dra, Toufik Bouddine, Khalid Sellam, Ali Ait Boughrous, Natalizia Miceli, Ammar B. Altemimi, and Francesco Cacciola
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Lactuca saligna L. ,phenolic compounds ,willow leaf lettuce ,HPLC-PDA/ESI-MS ,antioxidant activity ,antibacterial activity ,Organic chemistry ,QD241-441 - Abstract
Medicinal plants are considered a major source for discovering novel effective drugs. To our knowledge, no studies have reported the chemical composition and biological activities of Moroccan Lactuca saligna extracts. In this context, this study aims to characterize the polyphenolic compounds distributed in hydro-methanolic extracts of L. saligna and evaluate their antioxidant and antibacterial activities; in addition, in silico analysis based on molecular docking and ADMET was performed to predict the antibacterial activity of the identified phenolic compounds. Our results showed the identification of 29 among 30 detected phenolic compounds with an abundance of dicaffeoyltartaric acid, luteolin 7-glucoronide, 3,5-di-O-caffeoylquinic acid, and 5-caffeoylquinic acid with 472.77, 224.30, 196.79, and 171.74 mg/kg of dried extract, respectively. Additionally, antioxidant activity assessed by DPPH scavenging activity, ferric reducing antioxidant power (FRAP) assay, and ferrous ion-chelating (FIC) assay showed interesting antioxidant activity. Moreover, the results showed remarkable antibacterial activity against Escherichia coli, Salmonella typhimurium, Pseudomonas aeruginosa, Enterococcus faecalis, Staphylococcus aureus, and Listeria monocytogenes with minimum inhibitory concentrations between 1.30 ± 0.31 and 10.41 ± 0.23 mg/mL. Furthermore, in silico analysis identified three compounds, including Apigenin 7-O-glucuronide, Quercetin-3-O-glucuronide, and 3-p-Coumaroylquinic acid as potent candidates for developing new antibacterial agents with acceptable pharmacokinetic properties. Hence, L. saligna can be considered a source of phytochemical compounds with remarkable activities, while further in vitro and in vivo studies are required to explore the main biological activities of this plant.
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- 2024
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6. Elaboration and structural study of Ni(II), Cu(II), Zn(II) and Co(II) complexes based on the ligand [(N1Z,N2Z)-N1,N2-bis((1H-pyrrol-2-yl) methylene) ethane-1,2-diamine] with evaluation of antioxidant/antibacterial activities and cytotoxicity
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Ibtissam Elaaraj, Najia Moukrad, Aziz Bouymajane, Safae Er Raouan, Asmae Nakkabi, Oumayma Oulidi, Fouzia Rhazi Filai, Ibnsouda Koraichi Saad, Francesco Cacciola, Noureddine El Moualij, and Mohammed Fahim
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Ligand ,Complexes ,DPPH ,Antimitotic ,Genotoxic ,Hemolysis ,Chemistry ,QD1-999 - Abstract
In this contribution new Cobalt(II), Nickel(II), Copper(II) and Zinc(II) complexes based on the tetradentate ligand [(N1Z,N2Z)-N1,N2-bis((1H-pyrrol-2-yl)methylene)ethane-1,2-diamine] were synthesized and characterized by spectral techniques such as UV–Visible, infrared, nuclear magnetic resonance (NMR, 1H,13C), thermal analysis (DTA and TGA) and mass spectrometry. Results showed that Co (II) and Ni (II) complexes have an octahedral geometry, Zn (II) complex has a tetrahedral structure, and Copper (II) complex has a square planar geometry. Furthermore, based on the molar conductivity values, these complexes were considered as non-electrolytes except the copper complex. The synthesized ligand and their complexes were assessed for their antioxidant and antibacterial activities. All complexes showed greater antioxidant and antibacterial activities with respect to the ligand. In addition, the ligand and its complexes were also tested biologically first on the germination of Lepidium sativum seeds, which is a phytotoxic and antimitotic test, and on the hemolytic and genotoxic behavior of human blood cells. A total inhibition on germination and radicle growth of Lepidium sativum seeds treated with [CuL]Cl22H2O solution was recorded. However, the [CoLCl2]H2O, caused a complete hemolysis of red blood cells and total alteration of nuclei and membranes of leukocytes.
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- 2023
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7. A Comparative Study between Microwave Hydrodiffusion and Gravity (MHG) and Ultrasound-Assisted Extraction (UAE): Chemical and Biological Characterization of Polyphenol-Enriched Extracts from Aglianico Grape Pomace
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Giuseppina Crescente, Giovanni Cascone, Antonio Petruzziello, Aziz Bouymajane, Maria Grazia Volpe, Gian Luigi Russo, and Stefania Moccia
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grape pomace ,antioxidant compounds ,microwave hydrodiffusion and gravity extraction ,ultrasound-assisted extraction ,polyphenols ,antiproliferative activity ,Chemical technology ,TP1-1185 - Abstract
The wine industry produces large amounts of grape pomace (GP), a waste that needs to be disposed of properly. Bioactive compounds with high added value can be recovered from GP as an interesting strategy to reduce the environmental impact. Here, two different technologies were employed to recover polyphenol compounds from GP: microwave hydrodiffusion and gravity (MHG) and ultrasound-assisted extraction (UAE). The further purification of UAE and MHG extracts was carried out through solid-phase extraction (SPE) to obtain three fractions, F1, F2 and F3. ATR-FTIR analysis confirmed the presence of sugar and polysaccharide components in F1, as well as non-anthocyanin and anthocyanin compounds in F2 and F3, respectively. Also, the chemical profile was determined by HPLC-UV-DAD, identifying the presence of catechin in F2, and malvidin-3-O-glucoside chloride and cyanidin chloride derivative as the main anthocyanin compounds in F3. The fractions and their parental extracts were characterized for total phenolic content (TPC) and scavenger activity by in vitro assays. We found that F2-MHG and F3-MHG contained phenol contents 6.5 and 8.5 times higher than those of the parental non-fractionated extracts. Finally, F3-MHG (100 μg/mL, w/v) was shown to reduce the proliferation of HT-29 cells.
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- 2023
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8. Biodegradation of polyamide 6 by Lysinibacillus sp, Alcaligene faecalis and Enterococcus faecalis
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Oumayma Oulidi, Asmae Nakkabi, Aziz Bouymajane, Ibtissam Elaraaj, Fouzia Rhazi Filali, Mohammed Fahim, and Noureddine El Moualij
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Polyamide 6 ,Biodegradation ,Lysinibacillus sp ,Alcaligene faecalis ,Enterococcus faecalis ,Chemical engineering ,TP155-156 - Abstract
Polyamide 6 (PA6, polycaprolactam, Nylon 6) is a thermoplastic polymer widely used in construction, automotive, packaging, etc. It is a semi-crystalline polymer known for its good mechanical properties, and chemical, and thermal stability, with a low price, compared to the other polyamides. PA6 can be synthesized by anionic polymerization of ε-caprolactam, initiated by sodium hydride, and activated by N-acetylcaprolactam. This poorly biodegradable material has quickly proven to be a source of considerable pollution both visually and in terms of ecosystem preservation, particularly due to its increasing annual global production. This issue raises the need to develop environmentally friendly protocols for the degradation of this waste. One of the methods that can prove to be effective is the degradation by microorganisms. The objective of our research is to study the degradation of PA6 by the bacteria Lysinibacillus sp. (LB), Alcaligene faecalis (AF), and Enterococcus faecalis (EF) isolated from the landfill. The three bacteria, isolated and previously identified, were able to show proliferation in minimal media using PA6 as the sole source of carbon and nitrogen. The weight loss of the PA6 pellets was evaluated at 21% for L. bacillus, 27% for A. faecalis, and 13% for E. faecalis after 48 days of incubation. The biodegradation of PA6 was also confirmed by FTIR and SEM coupled to the elemental detector scanning EDS, which revealed the structural, morphological, and elemental changes that PA6 underwent.
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- 2022
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9. Electronic, Optical, Thermoelectric and Elastic Properties of RbxCs1−xPbBr3 Perovskite
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Elmustafa Ouaaka, Mustapha Aazza, Aziz Bouymajane, and Francesco Cacciola
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band structure ,density functional theory ,optical conductivity ,electrical conductivity ,Organic chemistry ,QD241-441 - Abstract
Inorganic halide perovskites of the type AMX3, where A is an inorganic cation, M is a metal cation, and X is a halide anion, have attracted attention for optoelectronics applications due to their better optical and electronic properties, and stability, under a moist and elevated temperature environment. In this contribution, the electronic, optical, thermoelectric, and elastic properties of cesium lead bromide, CsPbBr3, and Rb-doped CsPbBr3, were evaluated using the density functional theory (DFT). The generalized gradient approximation (GGA) in the scheme of Perdew, Burke, and Ernzerhof (PBE) was employed for the exchange–correlation potential. The calculated value of the lattice parameter is in agreement with the available experimental and theoretical results. According to the electronic property results, as the doping content increases, so does the energy bandgap, which decreases after doping 0.75. These compounds undergo a direct band gap and present an energies gap values of about 1.70 eV (x = 0), 3.76 eV (x = 0.75), and 1.71 eV (x = 1). The optical properties, such as the real and imaginary parts of the dielectric function, the absorption coefficient, optical conductivity, refractive index, and extinction coefficient, were studied. The thermoelectric results show that after raising the temperature to 800 K, the thermal and electrical conductivities of the compound RbxCs1−xPbBr3 increases (x = 0, 0.25, 0.50 and 1). Rb0.75Cs0.25PbBr3 (x = 0.75), which has a large band gap, can work well for applications in the ultraviolet region of the spectrum, such as UV detectors, are potential candidates for solar cells; whereas, CsPbBr3 (x = 0) and RbPbBr3 (x = 1), have a narrow and direct band gap and outstanding absorption power in the visible ultraviolet energy range.
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- 2023
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10. HPLC-PDA/ESI-MS Analysis of Phenolic Compounds and Bioactivities of the Ethanolic Extract from Flowers of Moroccan Anacyclus clavatus
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Mounia Chroho, Mustapha Aazza, Aziz Bouymajane, Yassine Oulad El Majdoub, Francesco Cacciola, Luigi Mondello, Touriya Zair, and Latifa Bouissane
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Anacyclus clavatus ,flower ethanolic extract ,total polyphenol content ,flavonoid content ,phenolic composition ,FRAP activity ,Botany ,QK1-989 - Abstract
In this work, polyphenols were extracted from Anacylus clavatus flowers using a hydroethanolic solvent, and the obtained extract was studied for its total phenol and flavonoid contents and evaluated for its antioxidant and antibacterial capacities. The contents of total phenols and flavonoids were measured by employing gallic acid and quercetin as references, respectively, and the phenolic composition analysis was conducted using high-performance liquid chromatography combined with a photodiode array and electrospray ionization mass spectrometry (HPLC-PDA/ESI-MS). The antioxidant capacity of the extracts was tested using a potassium ferric reducing antioxidant power (PFRAP) assay, and the antibacterial activity assay was carried out against Gram-negative bacteria (Escherichia coli and Salmonella typhimirium) and Gram-positive bacteria (Staphyloccocus aureus and Listeria monocytogenes) using the broth microdilution assay. The phenolic and flavonoid contents of the extracts equaled 9.53 ± 0.48 mg GAE/g dm and 1.31 ± 0.06 mg QE/g dm, respectively. The chromatographic analysis of the phenolic profile detected 26 phenolic compounds belonging to phenolic acids, flavones and flavonols, and with the caffeoylquinic acid derivatives being the major phenolic compounds present in 12 isomers. Only one organic compound, viz. citric acid, was found. The extracts exhibited interesting antioxidant activity. Bacteriostatic activity towards Escherichia coli and bactericidal activity against Salmonella typhimirium, Staphyloccocus aureus and Listeria monocytogenes were determined. This study revealed that Anacyclus clavatus flower extracts contain phenolic compounds with interesting bioactivities.
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- 2022
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11. Flavonoid Composition and Antibacterial Properties of Crocus sativus L. Petal Extracts
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Nadia Naim, Aziz Bouymajane, Yassine Oulad El Majdoub, Said Ezrari, Rachid Lahlali, Abdessalem Tahiri, Said Ennahli, Roberto Laganà Vinci, Francesco Cacciola, Luigi Mondello, and Ilham Madani
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Crocus sativus L. by-product ,flavonoid composition ,HPLC-PDA-ESI/MS ,antibacterial activity ,Organic chemistry ,QD241-441 - Abstract
Saffron petals, which are the main by-products of Crocus sativus L. (Iridaceae family), are produced in large quantities and are known for their many beneficial properties. In this regard, this study aims to investigate the phenolic composition and antibacterial properties of hydroethanolic extracts from Crocus sativus L. petals collected from Serghina (province of Boulmane) in Morocco. The phenolic profiles were characterized using high-performance liquid chromatography coupled to a photodiode array and electrospray ionization mass spectrometry (HPLC-PDA-ESI/MS). The antibacterial potential was evaluated against four bacterial strains potentially causing food-borne disease (Staphylococcus aureus, Salmonella typhimurium, Escherichia coli, and Listeria monocytogenes) using disc diffusion and broth micro-dilution assays. Results showed that a total of 27 phenolic compounds was detected in the Crocus sativus L. petal extracts, which were assigned to flavonoids (kaempferol, quercetin, isorhamnetin, and myricetin derivatives). The most abundant compound was represented by kaempferol-sophoroside isomer (20.82 mg/g ± 0.152), followed by kaempferol-sophoroside-hexoside (2.63 mg/g ± 0.001). The hydroethanolic extracts of Crocus sativus L. petals demonstrated bactericidal effects against Staphylococcus aureus and Listeria monocetogenes and bacteriostatic effects against Escherichia coli and Salmonella typhimurium. Therefore, the by-product Crocus sativus L. petal extracts might be considered as valuable sources of natural antibacterial agents with potential applications in the food and pharmaceutical industries.
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- 2022
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12. Study of the Effectiveness of Alumina and HDTMA/Alumina Composite in the Removal of Para-Nitrophenol and the Deactivation of Bacterial Effect of Listeria monocytogenes and Salmonella spp.
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Mustapha Aazza, Chadia Mounir, Hammou Ahlafi, Hamou Moussout, Aziz Bouymajane, Mounia Chroho, Filippo Giarratana, Luca Nalbone, and Francesco Cacciola
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adsorption ,Al2O3 ,HDTMA+/Al2O3 ,para-nitrophenol ,antibacterial activity ,Science - Abstract
Removal of para-nitrophenol (p-NP) from an aqueous solution was studied under various batch adsorption experiments, using alumina (Al2O3) and its composite hexadecyltrimethylammonium bromide (HDTMA+-Br−) as adsorbents. These were later characterized, before and after adsorption of p-NP, by thermal analysis (DSC-TG), X-ray diffraction (XRD), Fourier transform infrared (FTIR), and UV/Visible spectroscopies. The results show that HDTMA+/Al2O3 adsorbents have a greater affinity toward p-NP than Al2O3 alone. Linear and non-linear forms of kinetics and isotherms were used to analyze the experimental data obtained at different concentrations and temperatures. The results indicate that the pseudo-second order kinetic model provided the best fit to the experimental data for the adsorption of p-NP on both adsorbents, and that the intra-particle diffusion was not only the rate controlling step. Both the Langmuir and Redlich-Peterson (R-P) models were found to fit the sorption isotherm data well, but the Langmuir model was better. Physical adsorption of p-NP onto the adsorbents proved to be an endothermic and spontaneous process at high temperatures, which mainly involves a hydrogen bonding mechanism of interactions between p-NP and functional groups of adsorbents. The antibacterial activity of Al2O3, HDTMA+-Br− and HDTMA+/Al2O3 were evaluated against Listeria monocytogenes and Salmonella spp. strains using both disc diffusion and broth microdilution methods. The HDTMA+-Br− and HDTMA+/Al2O3 displayed a bacteriostatic effect against all tested strains of Listeria monocytogenes and Salmonella spp., while Al2O3 exhibited no bacterial effect against all bacterial strains tested.
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- 2022
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13. Phenolic Composition, Antioxidant and Antibacterial Activities of Extract from Flowers of Rosa damascena from Morocco
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Mounia Chroho, Aziz Bouymajane, Yassine Oulad El Majdoub, Francesco Cacciola, Luigi Mondello, Mustapha Aazza, Touriya Zair, and Latifa Bouissane
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Rosa damascena ,flowers ,HPLC-PDA-ESI/MS ,antioxidant activity ,antibacterial activity ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
Rosa damascena is referred to as the queen of roses due to its ornamental, flavoring, and perfuming uses, along with its recognized use in therapy. This study aimed to investigate the total phenols and flavonoids contents, the phenolic compounds, and study the antioxidant and antibacterial properties of the hydroethanolic extract from Rosa damascena flowers, collected from the Middle Atlas of Morocco (Khenifra). The total phenols and flavonoids were assessed using gallic acid and quercetin as standards, and the phenolic compounds were characterized using HPLC-PDA-ESI/MS. The antioxidant activity was evaluated by two methods, namely ferric reducing assay power and total antioxidant capacity. The broth microdilution method was employed to evaluate the antibacterial activity of extract against four bacteria (Escherichia coli, Salmonella typhimurium, Staphylococcus aureus, and Listeria monocytogenes). Up to 16 phenolic compounds belonging to tannins and flavonoids were positively identified in the Rosa damascena extract. The latter displayed high antioxidant activity and exhibited a bacteriostatic effect against Escherichia coli and a bactericidal effect against Salmonella typhimuriumn, Staphylococcus aureus, and Listeria monocytogenes. As a result, the flowers of Rosa damascena might be employed as natural agents in the pharmaceutical field.
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- 2022
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14. Determination of the Phenolic Profile, and Evaluation of Biological Activities of Hydroethanolic Extract from Aerial Parts of Origanum compactum from Morocco
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Mounia Chroho, Aziz Bouymajane, Mustapha Aazza, Yassine Oulad El Majdoub, Francesco Cacciola, Luigi Mondello, Touriya Zair, and Latifa Bouissane
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Origanum compactum ,phenolic compounds ,HPLC-PDA-ESI/MS ,antioxidant activity ,antibacterial activity ,Organic chemistry ,QD241-441 - Abstract
Origanum compactum belonging to the family Lamiaceae is widely used in food and pharmaceutical fields due to its biologically active substances. We aimed to investigate the total phenol and flavonoid contents and the phenolic composition, and to evaluate the antioxidant and antibacterial properties of hydroethanolic extract from of Origanum compactum. Total phenol and flavonoid contents were evaluated using gallic acid and quercetin as standards, respectively, and the phenolic profile was characterized using high-performance liquid chromatography coupled to a photodiode array and electrospray ionization mass spectrometry (HPLC-PDA-ESI/MS). The antioxidant activity was determined by two methods: ferric reducing power (FRAP) assay and the phosphomolybdate method. The antibacterial effect was evaluated against four bacteria (Escherichia coli, Salmonella typhimurium, Staphylococcus aureus and Listeria monocytogenes) using the broth microdilution method. The findings show that the total phenolic and flavonoid contents were 107.789 ± 5.39 mg GAE/g dm and 14,977 ± 0.79 mg QE/g dm, respectively. A total of sixteen phenolic compounds belonging to phenolic acids and flavonoids were detected. Furthermore, the extract showed strong antioxidant activity, and displayed a bacteriostatic effect against Escherichia coli and Salmonella typhimuriumn, and a bactericidal effect against Staphylococcus aureus and Listeria monocytogenes. Therefore, this study reveals that Origanum compactum extracts display potential as antibacterial and natural antioxidant agents for fighting against pathogenic bacteria and preventing oxidative stress.
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- 2022
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15. Occurrence, antimicrobial resistance, serotyping and virulence genes of Listeria monocytogenes isolated from foods
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Aziz Bouymajane, Fouzia Rhazi Filali, Said Oulghazi, Nada Lafkih, Abdelaziz Ed-Dra, Amal Aboulkacem, Abdallah El Allaoui, Bouchra Ouhmidou, and Mohieddine Moumni
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Foods ,Listeria monocytogenes ,Serotypes ,Virulence genes ,Multidrug-resistance ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Listeria monocytogenes is a pathogen contaminated food, it is the cause of listeriosis worldwide. The aims of this study were to investigate the occurrence, antimicrobial resistance, serotyping and virulence genes of L. monocytogenes isolated from foods in Meknes city of Morocco. From June 2017 to May 2018, 520 food samples were randomly collected from a traditional market and two overcrowded popular neighborhoods (Lahdim and Hamria) and subjected to the detection of L. monocytogenes. Then, the antimicrobial susceptibility of the isolated strains were evaluated using the standard disk diffusion method and the determination of serotypes and virulence genes was performed by PCR. The results showed the detection of L. monocytogenes in fifteen (2.9%) of 520 samples, including three (5.7%) isolates in traditional whey, raw minced meat and raw sausage, two (3.8%) in raw milk and one (1.9%) in smen (traditional butter), raw bovine meat, raw poultry meat and raw fish, while salads and rayeb (traditional coagulated milk) were not contaminated. Among the fifteen isolated L. monocytogenes, nine (60%) belonged to the serogroup (1/2a, 1/2c, 3a and 3c), two (13.3%) belonged to the serogroup (1/2b, 3b, 4b and 4d) and four (26.6%) do not belong to any studied serogroup. Furthermore, fifteen (100%) isolates showed the presence of actA gene, fourteen (93.3%) harbored hlyA, prfA and plcB genes, thirteen (86.7%) carried inlA and inlC genes and twelve (80%) showed inlJ gene. The antimicrobial susceptibility analysis showed that the isolated strains were more resistant to amoxicillin/clavulanic acid (67.0%), erythromycin (60.0%), sulphamethoxazole (40.0%), ampicillin and sulphamethoxazole/trimethoprim (33.0%) and tetracycline (20.0%). Furthermore, 66.7% (10/15) were multidrug-resistant. From this study, we can conclude that foods marketed in Meknes city were contaminated by multidrug-resistant strains of L. monocytogenes harboring virulence genes, which may cause a serious risk to public health.
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- 2021
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16. Antioxidant and Antimicrobial Activity of Polyphenols Extracted after Adsorption onto Natural Clay 'Ghassoul'
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Safae Allaoui, Mohammed Naciri Bennani, Hamid Ziyat, Omar Qabaqous, Najib Tijani, Najim Ittobane, Mohammed Barbouchi, Aziz Bouymajane, and Fouzia Rhazi Filali
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Chemistry ,QD1-999 - Abstract
Natural polyphenols contained in olive mill wastewaters (OMW) have been usually associated with great bioactive properties as “antioxidants”. In this work, we recovered the polyphenols after adsorption onto natural clay “ghassoul” by different solvents: water, ethyl acetate, and methanol (PPW, PPA, and PPM, respectively) to avoid environmental pollution. Also, we tested the antioxidant activity of the extracted polyphenols by two methods: 1,1-diphenyl-2-picrylhydrazyl (DPPH) and total antioxidant capacity (TAC). Then, we analyzed antimicrobial activity by the microdilution technique to determine at the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). The OMW of the Fez-Meknes region has a very acidic pH, considerable amounts of mineral matter, and a high concentration of polyphenols and organic content. The results of the test from DPPH showed good antiradical potential for polyphenols extracted with water, but the TAC showed an important capacity for all extracts unless PPA. The antibacterial activity is not the same on the four bacteria studied (Escherichia coli, Salmonella sp, Staphylococcus aureus, and Enterococcus faecalis), and all extracts inhibit most tested germs that do not have the same MIC and the same sensitivity. Only the PPW showed the minimum bactericidal concentration (MBC) that is equal to 0.290 mg/mL for Salmonella sp and Staphylococcus aureus, which confirms that the extraction by water of the adsorbed polyphenols is an original solution to recover the polyphenols and also to obtain a natural phenolic antioxidant which can be used in the pharmaceutical, nourishment, and cosmetic industry.
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- 2020
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17. Chemical characterization and adsorption of oil mill wastewater on Moroccan clay in order to be used in the agricultural field
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Younes Dehmani, Abdelaziz Ed-Dra, Omar Zennouhi, Aziz Bouymajane, Fouzia Rhazi Filali, Laila Nassiri, and Sadik Abouarnadasse
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Agricultural science ,Environmental science ,Materials science ,Oil mill wastewater ,Adsorption ,Moroccan clay ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Oil mill wastewater (OMW) is the main liquid discharge from oil mills, it is considered as a dangerous pollutant due to its toxic chemical compounds which are unloaded directly in the environment without any treatment. The aims of this study were to evaluate the effectiveness of OMW adsorption on clay as a good method for the elimination of toxic chemical compounds and to study the application of treated OMW as an irrigation source in agricultural field. For this, Clay was collected from the city of Agourai (Meknes region, Morocco) and characterized by X-ray diffraction, X-ray fluorescence spectrometry, BET and FTIR analysis. Moreover, the treated OMW was analyzed using UHPLC-ESI-MS and the determination of total phenolic content (TPC) was also performed. However, the application of the treated OMW in agricultural field was performed by the determination of its effect on the germination of Lepidium sativum seeds (in vitro) and as a source of irrigation of Vicia faba plants (in situ). The results of this study showed that OMW had the following physicochemical characteristics: average pH of 4.88, TPC of 4.75 g/l, COD of 80 g/l, BOD5 of 18.72 g/l, conductivity of 16.05 cm-1, dry matter of 135.7 g/l and volatile matter of 58.7 g/l. The adsorption on clay had increased the pH from 4.88 to 6.14 and reduced significantly the organic matter (42% of COD and 57.4% of phenolic compounds). UHPLC-ESI-MS analysis showed the presence of a wide variety of organic compounds in OMW, with the appearance of new compounds after adsorption. Moreover, the use of treated OMW as a source of irrigation showed a significant effect on the germination of Lepidium sativum seeds and the growth of Vicia faba plants. From this study, we can conclude that the adsorption on clay is a good method for the treatment of OMW, which became non-toxic for environment and can be used as a source of irrigation in agricultural field.
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- 2020
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18. Antibiotic Susceptibility profile of Staphylococcus aureus isolated from sausages in Meknes, Morocco
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Abdelaziz Ed-Dra, Fouzia Rhazi Filali, Aziz Bouymajane, Faouzia Benhallam, Abdellah El Allaoui, Abdellah Chaiba, and Filippo Giarratana
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antimicrobial resistance ,foodborne disease ,infection ,sausages ,Staphylococcus aureus ,Animal culture ,SF1-1100 ,Veterinary medicine ,SF600-1100 - Abstract
Background and Aim: Staphylococcus aureus is one of the most common causes of foodborne disease worldwide, due to the consumption of food contaminated by their toxins. This study aimed to determine the prevalence and the antimicrobial resistance of S. aureus isolated from sausages in Meknes city of Morocco. Materials and Methods: A total of 156 samples (Beef sausages, Turkey sausages, and Artisanal sausages "Merguez") were collected from different shopping sites (butchery, supermarket, street vendors, and weekly market "Souk") and used for the isolation of S. aureus. All the isolated strains were tested for their antimicrobials resistance to 16 antibiotics. Results: Our results showed the presence of S. aureus in 63 samples (40.38%). Furthermore, the antimicrobial resistance study showed that 84.13% of isolated S. aureus were resistant to streptomycin, 76.20% to tetracycline, 42.86% to ampicillin, 41.27% to doxycycline, 38.1% to penicillin G, and 19.05% to chloramphenicol with the presence of 25 different phenotypic profiles. However, all isolated strains were sensitive to oxacillin, cefoxitin, gentamicin, and vancomycin. Conclusion: The findings of this study revealed consumption of sausages as a potential risk of foodborne poisonings because of its contamination with the multi-resistant strains of S. aureus. Moreover, this contamination is related to the season, sampling sites and the origin of the raw material.
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- 2018
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19. Characterization of Phenolic Compounds, Vitamin E and Fatty Acids from Monovarietal Virgin Olive Oils of 'Picholine marocaine' Cultivar
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Aziz Bouymajane, Yassine Oulad El Majdoub, Francesco Cacciola, Marina Russo, Fabio Salafia, Alessandra Trozzi, Fouzia Rhazi Filali, Paola Dugo, and Luigi Mondello
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virgin olive oils ,Picholine marocaine ,phenolic compounds ,vitamin E ,fatty acids ,HPLC-PDA/ESI-MS ,Organic chemistry ,QD241-441 - Abstract
Olive oil is an important product in the Mediterranean diet, due to its health benefits and sensorial characteristics. Picholine marocaine is the most cultivated variety in Morocco. The present research aims to evaluate the phenolic compounds, vitamin E and fatty acids of commercial Picholine marocaine virgin olive oils (VOOs) from five different North Moroccan provinces (Chefchaouen, Taounate, Errachidia, Beni Mellal and Taza), using HPLC-photodiode array (PDA)/electrospray ionization (ESI)-MS, normal phase (NP)-HPLC/ fluorescence detector (FLD) and GC-flame ionization detector (FID)/MS, respectively. The obtained results showed an average content of 130.0 mg kg−1 of secoiridoids (oleuropein aglycone, 10-hydroxy-oleuropein aglycone and ligstroside aglycone, oleocanthal and oleacein), 108.1 mg kg−1 of phenolic alcohols (tyrosol and hydroxytyrosol), 34.7 mg kg−1 of phenolic acids (caffeic acid, ferulic acid and elenolic acid), and 8.24 mg kg−1 of flavonoids (luteolin, luteolin glucoside, apigenin). With regard to vitamin E, α-tocopherol was the most abundant vitamin E (57.9 mg kg−1), followed by α-tocotrienol (2.5 mg kg−1), γ-tocopherol (4.5 mg kg−1) and β-tocopherol (1.9 mg kg−1), while δ-tocopherol was not detected. Moreover, 14 fatty acids were found and, among them, oleic acid (76.1%), linoleic acid (8.1%) palmitic acid (8.7%) and stearic acid (2.5%) were the major fatty acids detected. Finally, heat map and principal component analysis allowed us to classify the studied provinces in terms of VOO chemical composition: Chefchaouen (tyrosol and hydroxytyrosol), Taounate (oleuropein aglycone), Errachidia (ferulic acid, w-3 and w-6), Beni Mellal (oleocanthal) and Taza (luteolin and oleic acid).
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- 2020
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20. Chemical profile, antibacterial, antioxidant and insecticidal properties of the essential oil from Tetraclinis articulata (Vahl) masters cones
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Fatima Zahra Sadiki, Aziz Bouymajane, Mohammed Sbiti, Souhail Channaoui, Giuseppe Micalizzi, Francesco Cacciola, Paola Dugo, Luigi Mondello, and Mostafa El Idrissi
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General Chemistry - Published
- 2022
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21. Chemical composition, antioxidant/antibacterial activities and ADMET study of the essential oil isolated from the aerial parts of Ziziphora hispanica grown in Morocco
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Mustapha Cherrate, Reda EL-Mernissi, Aziz Bouymajane, Fouzia Rhazi Filali, Emanuela Trovato, Ghizlane Echchgadda, Abdellah Maissour, Mohammed Aziz Ajana, Paola Dugo, Francesco Cacciola, Luigi Mondello, and Kacem Makroum
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General Chemistry - Abstract
The present study aimed to characterize the chemical composition and evaluate the antioxidant and antibacterial activities along with the absorption, distribution, metabolism, excretion, toxicity (ADMET) study of the essential oil of Ziziphora hispanica (ZH-EO), collected from the Middle Atlas of Morocco (Boulmane). Results showed that a total of 119 volatile components, characterized by GC-FID and GC-MS analysis, which represents the 88.7% of the total of ZH-EO. ZH-EO displayed a bactericidal effect against Listeria monocytogenes, Pseudomonas aeruginosa and Enterococcus faecalis, and a bacteriostatic effect against Staphylococcus aureus. Furthermore, ZH-EO exhibited strong antioxidant activity against DPPH radical (IC50 = 1.3 mg/mL). The ADMET prediction showed good pharmacokinetic properties of the tested components. The findings obtained from this study suggest that Ziziphora hispanica essential oil could represent a source of bioactive molecules with antioxidant and antibacterial potential in the prevention against diseases related to oxidative stress and pathogenic bacteria.
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- 2023
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22. First Survey on Behavior and Preferences Towards Honey Consumption by the Eastern Central Moroccan Population
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Toufik Bouddine, Mohamed Ben Aziz, Aziz Zouhri, Aziz Bouymajane, Mohamed Reda Kachmar, Rafik El mernissi, Mourad Akdad, Soukaina Arif, Farid Khallouki, and Lhoussain Hajji
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- 2023
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23. Development of a New Biocomposite Polystyrene- Acorn Pericarps Biodegradable by Enterococcus Faecalis
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Aziz Bouymajane, Maria Jabri, Ilyasse Loulidi, Abderahim Kali, Abdelouahed Amar, Fouzia Rhazi Filali, and Fatima Boukhlifi
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- 2023
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24. Performance of first derivative UV/Visible spectra for kinetic and isothermal study of simultaneous adsorption of o-nitrophenol and p-nitrophenol onto Al2O3 and HDTMA+/Al2O3 composite
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Mustapha Aazza, Chadia Mounir, Hammou Ahlafi, Aziz Bouymajane, and Francesco Cacciola
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Materials Chemistry ,Physical and Theoretical Chemistry ,Condensed Matter Physics ,Spectroscopy ,Atomic and Molecular Physics, and Optics ,Electronic, Optical and Magnetic Materials - Published
- 2023
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25. Phytochemical profile, in vivo anti-inflammatory and wound healing activities of the aqueous extract from aerial parts of Cistus ladanifer L
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Imad Adadi, Rachida El Ayadi, Amar Bentayeb, Hicham Aaziz, Aziz Bouymajane, Ammar B. Altemimi, Francesco Cacciola, and Hamid El Ibaoui
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Inflammation ,Wound Healing ,Plant Extracts ,Clinical Biochemistry ,Phytochemicals ,Anti-Inflammatory Agents ,Cistus ,Pharmaceutical Science ,Plant Components, Aerial ,Analytical Chemistry ,Rats ,Drug Discovery ,Animals ,Edema ,Spectroscopy - Abstract
Cistus ladanifer L. is a Mediterranean shrub, well recognized for its many pharmacological properties. The present study aimed to investigate the phytochemical profile, the in vivo anti-inflammatory and the wound healing activities of the aqueous extract from its aerial parts (AECL). The in vivo anti-inflammatory and the wound healing properties were evaluated by carrageenan-induced paw edema (CAECL) and burn wound in rats, respectively. The results showed that six compounds belonging to flavonoids and tannins were identified in the AECL extract. Moreover, the AECL administrated orally at a dose of 500 mg/kg significantly reduced edema with a percentage of 72 % in terms of inflammation inhibition, using indomethacin as reference drug. On the other hand, when administrated topically, both doses of CAECL cream (5 % and 10 %) manifested a significant anti-inflammatory action using diclofenac as reference drug with a percentage reduction of inflammation that reached 85 % (CAECL-10). As far as wound healing is concerned, the CAECL showed higher effect at a dose of 10 % (CAECL-10) producing a wound contraction of 96 %, similar to that of the reference drug. The findings of the present study indicate that the aqueous extract from aerial parts of Cistus ladanifer L. display strongly anti-inflammatory and wound healing effects to be advantageously used for medical purposes.
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- 2022
26. Prevalence, characterization and antimicrobial resistance of Listeria monocytogenes isolated from beef meat in Meknes city, Morocco
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Meryem Boukili, Aziz Bouymajane, Majida Sefiani, Mohieddine Moumni, Fouzia Rhazi Filali, and Nada Lafkih
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Microbiology (medical) ,Serotype ,Veterinary medicine ,Imipenem ,General Immunology and Microbiology ,Epidemiology ,Sulfamethoxazole ,Public Health, Environmental and Occupational Health ,Virulence ,Biology ,medicine.disease_cause ,Antimicrobial ,Infectious Diseases ,Antibiotic resistance ,Listeria monocytogenes ,Amikacin ,medicine ,medicine.drug - Abstract
Introduction Listeria monocytogenes is one of the most pathogenic bacteria related to the consumption of contaminated food. This study aims to determine the prevalence of L. monocytogenes in raw beef meat in Meknes city of Morocco, to evaluate its pathogenicity and resistance to antimicrobials. Methods During four seasons, a total of 140 samples were collected from supermarkets, butcheries and Souk (weekly traditional market). The PCR method was used to examine the presence of specific and virulence genes in the isolated strains, and also to identify their serotypes. The antimicrobial resistance was determined. Results The results show a prevalence of 7.14% which depends on retail sites and also on the season's variation. The majority of the strains were detected in butcheries (6 strains), and supermarkets (4 strains). Moreover, the majority of strains were detected during summer (50%). Concerning virulence genes, the seven researched genes were detected in 100% of isolated strains. The majority of strains were of the (1/2a, 1/2c, 3a and 3c) serogroup (70%), while two of them were of the (1/2b, 3b, 4b and 4d) serogroup (20%). All isolates were resistant to at least one antimicrobial, while three strains were resistant to nine tested antimicrobials. However, they were highly susceptible to amikacin, imipenem, gentamicin, sulfamethoxazole and chloramphenicol. Conclusions According to results, isolated L. monocytogenes from analyzed beef meat shows a high level of pathogenicity and resistance to the most used antimicrobials in listeriosis therapy, which calls for the severe application of quality systems at the slaughterhouses and retail sites level.
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- 2020
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27. Phenolic Compounds, Antioxidant and Antibacterial Activities of Extracts from Aerial Parts of Thymus zygis subsp. gracilis , Mentha suaveolens and Sideritis incana from Morocco
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Aziz Bouymajane, Fouzia Rhazi Filali, Yassine Oulad El Majdoub, Mohamed Ouadik, Rahou Abdelilah, Emilia Cavò, Natalizia Miceli, Maria Fernanda Taviano, Luigi Mondello, and Francesco Cacciola
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Molecular Medicine ,Bioengineering ,General Chemistry ,General Medicine ,Molecular Biology ,Biochemistry - Published
- 2022
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28. Preparation, spectral characterization, crystal structure and antibacterial activity of two new supramolecular complexes [Ni(phen)2Cl(H2O)]2(PF6)2.2caf.H2O (I), [Ni(phen)2(H2O)2]2(PF6)4. 3caf.4H2O(II) constructed via hydrogen bond linking
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Hicham EL Hamdani, Mohammed EL Amane, Aziz Bouymajane, and Najia El Hamzaoui
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Inorganic Chemistry ,Organic Chemistry ,Spectroscopy ,Analytical Chemistry - Published
- 2023
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29. Phenolic Compounds, Antioxidant and Antibacterial Activities of Extracts from Aerial Parts of Thymus zygis subsp. gracilis, Mentha suaveolens and Sideritis incana from Morocco
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Aziz, Bouymajane, Fouzia Rhazi, Filali, Yassine Oulad, El Majdoub, Mohamed, Ouadik, Rahou, Abdelilah, Emilia, Cavò, Natalizia, Miceli, Maria Fernanda, Taviano, Luigi, Mondello, and Francesco, Cacciola
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Morocco ,Lamiaceae ,Plant Extracts ,Sideritis ,Plant Components, Aerial ,Antioxidants ,Anti-Bacterial Agents ,Mentha - Abstract
Thymus zygis subsp. gracilis, Mentha suaveolens and Sideritis incana (Lamiaceae family) are well recognized for their medicinal, pharmaceutical and aromatic properties. The present study aimed to investigate for the first time the phenolic composition, the antioxidant and antibacterial properties of the extracts obtained from the aerial parts of these species collected from the Ifrane region of Morocco. The phenolic compounds were determined using high-performance liquid chromatography coupled to photodiode array and electrospray ionization mass spectrometry (HPLC-PDA-ESI/MS). The antioxidant activity was investigated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, reducing power and ferrous ion chelating assays. The antibacterial activity was evaluated against three Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa and Salmonella typhimurium) and three Gram-positive bacteria (Staphylococcus aureus, Enterococcus faecalis and Listeria monocytogenes). A total of thirty-three, sixteen and thirteen phenolic compounds were positively identified and characterized in T. zygis subsp. gracilis, M. suaveolens and S. incana extracts, respectively. Among the extracts, M. suaveolens exhibited the highest antioxidant activity, followed by S. incana and T. zygis subsp. gracilis in both DPPH and reducing power assays; all extracts showed the lowest activity in the chelating assay. In addition, all extracts demonstrated a bactericidal effect against Gram-positive bacteria and bacteriostatic effect against Gram-negative bacteria. Therefore, the aerial parts of Moroccan T. zygis subsp. gracilis, M. suaveolens and S. incana might be considered as a valuable source of natural antioxidant and antibacterial agents with potential application in food and pharmaceutical industries.
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- 2021
30. Chemical profile, antibacterial, antioxidant, and anisakicidal activities of Thymus zygis subsp. gracilis essential oil and its effect against Listeria monocytogenes
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Aziz, Bouymajane, Fouzia Rhazi, Filali, Abdelaziz, Ed-Dra, Mustapha, Aazza, Luca, Nalbone, Filippo, Giarratana, Filippo, Alibrando, Natalizia, Miceli, Luigi, Mondello, and Francesco, Cacciola
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Thymus Plant ,Lamiaceae ,Oils, Volatile ,Animals ,Microbial Sensitivity Tests ,General Medicine ,Listeria monocytogenes ,Microbiology ,Antioxidants ,Anti-Bacterial Agents ,Food Science - Abstract
In this work, the chemical composition of Thymus zygis subsp. gracilis collected from Ifrane, Morocco, along with the evaluation of the antibacterial, anti-biofilm of Listeria monocytogenes activities, larvicidal effect against L3 larvae of Anisakis, and antioxidant properties of its essential oil (TZG-EO), are reported. GC-MS and GC-FID analyses highlighted the presence of 84 volatile components and strong bactericidal and anti-biofilm activities against L. monocytogenes at a concentration of 0.02 % were demonstrated. Also, larvicidal effect against Anisakis larvae at concentrations of 0.01 and 0.005 % was attained leading to the death of all tested larvae within 4 h. The in situ antibacterial activity of TZG-EO (0.01 and 0.005 %) in smoked fish showed high efficiency against L. monocytogenes growth. TZG-EO could be used as potential antibacterial and larvicidal agents for fighting against foodborne pathogens and extending shelf life of food products.
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- 2022
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31. Chemical composition, antioxidant capacity and antibacterial action of five Moroccan essential oils against Listeria monocytogenes and different serotypes of Salmonella enterica
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Abdelaziz Ed-Dra, Badr Zekkori, Filippo Giarratana, Luca Nalbone, Aziz Bouymajane, Alessandro Giuffrida, Amar Bentayeb, Najla Trabelsi, Fouzia Rhazi Filali, Filippa Lamberta, and Vittorio Lo Presti
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0301 basic medicine ,Salmonella ,030106 microbiology ,Mentha suaveolens ,Mentha pulegium ,Origanum majorana ,Rosmarinus officinalis ,Salvia officinalis ,Sub-lethally injured bacteria ,Microbial Sensitivity Tests ,medicine.disease_cause ,Serogroup ,Microbiology ,Antioxidants ,03 medical and health sciences ,Camphor ,chemistry.chemical_compound ,food ,Listeria monocytogenes ,Anti-Infective Agents ,medicine ,Oils, Volatile ,Food science ,biology ,Salmonella enterica ,Origanum ,Antimicrobial ,biology.organism_classification ,food.food ,Anti-Bacterial Agents ,030104 developmental biology ,Infectious Diseases ,chemistry ,Antibacterial activity - Abstract
Essential oils (EOs) obtained from aromatic plants are rich in natural components with interesting antimicrobial effects. The aim of this study was to evaluate the chemical composition of EOs extracted from Origanum majorana (OM-EO), Mentha suaveolens (MS-EO), Rosmarinus officinalis (RO-EO), Salvia officinalis (SO-EO) and Mentha pulegium (MP-EO). Their antioxidant properties and antibacterial activity against Listeria monocytogenes and different serotypes of Salmonella enterica subsp. enterica were also studied. The EOs were extracted from plants by hydro-distillation and their chemical composition was determined by GC-MS. Terpinen-4-ol, 1,8-Cineole, Camphor, Limonene and Cinerone were the main chemical components found in OM-EO, RO-EO, SO-EO, MP-EO and MS-EO, respectively. To the best of our knowledge, Limonene and Cinerone were reported, for the first time, as the major components of MP-EO and MS-EO. Moreover, our results showed that MS-EO had the best antioxidant activity with an IC50 of 0.78 ± 0.05 mg/mL, EC50 of 1.53 ± 0.07 mg/mL, and RC50 of 0.98 ± 0.04 mg/mL, and the higher antibacterial activity using microdilution broth method with MIC of 0.5% for Salmonella and 0.25% for L. monocytogenes, while OM-EO had the best antibacterial activity using disc diffusion method (inhibition diameters ranged between 15.3 ± 0.3 mm and 18.5 ± 0.3 mm for Salmonella and between 20.1 ± 0.2 mm and 25.4 ± 0.4 mm for L. monocytogenes). However, OM-EO and MS-EO present the higher percentage of sub-lethally injured cells against S. enterica (5.50 ± 0.11%) and L. monocytogenes (5.23 ± 0.07%), respectively. From this study, we can conclude that the investigated EOs are rich in components with interesting antibacterial activity and they could be applied in food preparations as natural preservatives to extend the shelf life of food products and to inhibit the growth of food-borne pathogens.
- Published
- 2020
32. Comparative Study of Chemical -Composition and Physico-Chemical Characteristics of Thymus Vulgaris's Essential Oil Harvested from Deraa-Tafilalet's Region (Morocco) with Previous Studies
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Elgamouz Sabah, mosatafa Elidrissi, Aziz Bouymajane, Bouzekri Omayma, and M’barek Choukrad
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biology ,Gram-positive bacteria ,Thymus vulgaris ,biology.organism_classification ,Antimicrobial ,law.invention ,chemistry.chemical_compound ,chemistry ,law ,Lamiaceae ,Carvacrol ,Food science ,Medicinal plants ,Saponification ,Essential oil - Abstract
Thymus vulgaris which is known as Thym, Zitra in Arabic it belongs to the Lamiaceae family. It is one of many wild aromatic medicinal plants, which grows naturally in Morocco. In our study we chose the one harvested from South East Morocco precisely in Deraa-Tafilalet’s region. The essential oil is obtained by Hydrodistillation using Clevenger device with an yield of 1, 3 % using CG/MS analysis that Carvacrol (45,85%) and endo-Borneol (22,46%) are the components with the highest rate. Acid and saponification index resulting indicate that Thymus vulgaris’s essential oil contains not much of free acids reason why essential oil doesn’t regroup saturated compounds.The results of antimicrobial activity of putting thymus vulgaris’s essential oil against Gram positive bacteria ( Staphylococcus aureus, Salmonella ), Gram negative bacteria ( Escherichia coli ) and Candida albicans, showed that Thymus vulgaris’s essential oil has a bactericidal activity, against all the microorganisms.
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- 2020
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33. Antioxidant and Antimicrobial Activity of Polyphenols Extracted after Adsorption onto Natural Clay 'Ghassoul'
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Mohammed Barbouchi, Najib Tijani, Aziz Bouymajane, Safae Allaoui, Najim Ittobane, Omar Qabaqous, Hamid Ziyat, Fouzia Rhazi Filali, and Mohammed Naciri Bennani
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Minimum bactericidal concentration ,Antioxidant ,Article Subject ,Chemistry ,DPPH ,medicine.medical_treatment ,Ethyl acetate ,food and beverages ,Environmental pollution ,04 agricultural and veterinary sciences ,General Chemistry ,010501 environmental sciences ,040401 food science ,01 natural sciences ,chemistry.chemical_compound ,Minimum inhibitory concentration ,0404 agricultural biotechnology ,Polyphenol ,medicine ,Food science ,Antibacterial activity ,QD1-999 ,0105 earth and related environmental sciences - Abstract
Natural polyphenols contained in olive mill wastewaters (OMW) have been usually associated with great bioactive properties as “antioxidants”. In this work, we recovered the polyphenols after adsorption onto natural clay “ghassoul” by different solvents: water, ethyl acetate, and methanol (PPW, PPA, and PPM, respectively) to avoid environmental pollution. Also, we tested the antioxidant activity of the extracted polyphenols by two methods: 1,1-diphenyl-2-picrylhydrazyl (DPPH) and total antioxidant capacity (TAC). Then, we analyzed antimicrobial activity by the microdilution technique to determine at the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). The OMW of the Fez-Meknes region has a very acidic pH, considerable amounts of mineral matter, and a high concentration of polyphenols and organic content. The results of the test from DPPH showed good antiradical potential for polyphenols extracted with water, but the TAC showed an important capacity for all extracts unless PPA. The antibacterial activity is not the same on the four bacteria studied (Escherichia coli, Salmonella sp, Staphylococcus aureus, and Enterococcus faecalis), and all extracts inhibit most tested germs that do not have the same MIC and the same sensitivity. Only the PPW showed the minimum bactericidal concentration (MBC) that is equal to 0.290 mg/mL for Salmonella sp and Staphylococcus aureus, which confirms that the extraction by water of the adsorbed polyphenols is an original solution to recover the polyphenols and also to obtain a natural phenolic antioxidant which can be used in the pharmaceutical, nourishment, and cosmetic industry.
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- 2020
34. Antibiotic Susceptibility profile of Staphylococcus aureus isolated from sausages in Meknes, Morocco
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Faouzia Benhallam, Abdellah Chaiba, Abdellah El Allaoui, Aziz Bouymajane, Abdelaziz Ed-Dra, Filippo Giarratana, and Fouzia Rhazi Filali
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0301 basic medicine ,Staphylococcus aureus ,Veterinary medicine ,030106 microbiology ,Biology ,medicine.disease_cause ,SF1-1100 ,03 medical and health sciences ,Antibiotic resistance ,Ampicillin ,SF600-1100 ,medicine ,antimicrobial resistance ,Cefoxitin ,General Veterinary ,foodborne disease ,Antimicrobial ,infection ,Animal culture ,Penicillin ,030104 developmental biology ,sausages ,antimicrobial resistance, foodborne disease, infection, sausages, Staphylococcus aureus ,Vancomycin ,Gentamicin ,medicine.drug - Abstract
Background and aims Staphylococcus aureus is one of the most common causes of foodborne disease worldwide, due to the consumption of food contaminated by their toxins. This study aimed to determine the prevalence and the antimicrobial resistance of S. aureus isolated from sausages in Meknes city of Morocco. Materials and methods A total of 156 samples (Beef sausages, Turkey sausages, and Artisanal sausages "Merguez") were collected from different shopping sites (butchery, supermarket, street vendors, and weekly market "Souk") and used for the isolation of S. aureus. All the isolated strains were tested for their antimicrobials resistance to 16 antibiotics. Results Our results showed the presence of S. aureus in 63 samples (40.38%). Furthermore, the antimicrobial resistance study showed that 84.13% of isolated S. aureus were resistant to streptomycin, 76.20% to tetracycline, 42.86% to ampicillin, 41.27% to doxycycline, 38.1% to penicillin G, and 19.05% to chloramphenicol with the presence of 25 different phenotypic profiles. However, all isolated strains were sensitive to oxacillin, cefoxitin, gentamicin, and vancomycin. Conclusion The findings of this study revealed consumption of sausages as a potential risk of foodborne poisonings because of its contamination with the multi-resistant strains of S. aureus. Moreover, this contamination is related to the season, sampling sites and the origin of the raw material.
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- 2018
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35. Occurrence, molecular and antimicrobial resistance of Enterococcus spp. isolated from raw cow’s milk trade by street trading in Meknes city, Morocco
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Khalid Sendide, Faouzia Benhallam, Jaouad Anissi, Aziz Bouymajane, Abdallah El Allaoui, Abdelaziz Ed-Dra, Mohieddine Moumni, Bouchra Ouhmidou, Said Oulghazi, and Fouzia Rhazi Filali
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Microbiology (medical) ,education.field_of_study ,Veterinary medicine ,General Immunology and Microbiology ,biology ,Epidemiology ,Population ,Public Health, Environmental and Occupational Health ,biochemical phenomena, metabolism, and nutrition ,Raw milk ,biology.organism_classification ,Enterococcus durans ,Enterococcus faecalis ,Infectious Diseases ,Antibiotic resistance ,Enterococcus ,Enterococcus hirae ,education ,Enterococcus faecium - Abstract
Background Enterococcus spp. belongs to a group of pathogens which are responsible for serious infections. This study aims at highlighting the raw milk microbiological contamination and at providing data for prevalence and antimicrobial resistance of Enterococcus spp. isolated from raw cow's milk marketed (without any pasteurization) by street traders. Methods During the period of May 2015 through April 2016, 150 cow's raw milk samples were collected from street traders in Meknes city. They were examined for the identification of Enterococcus spp. using biochemical tests and 16S rRNA gene sequencing. The antimicrobial susceptibility of the isolates was determined. Results The results showed that 11.3% (17/150) of samples were positive for the presence of Enterococcus spp., of which 64.7% were identified as Enterococcus faecalis, 17.6% as Enterococcus faecium, 11.8% as Enterococcus durans and 5.9% as Enterococcus hirae. The antimicrobial susceptibility showed that all Enterococcus spp. were resistant to ampicillin. The species E. faecalis, E. faecium, E. durans and E. hirae were resistant to streptomycin, with percentages of 52.9% (9/17), 11.8% (2/17), 11.8% (2/17), and 5.9% (1/17) respectively. All isolated strains of E. faecalis and E. faecium were resistant to tetracycline. The multiple antibiotic resistance index was elevated in the majority of Enterococcus spp., reaching values higher than 0.5, indicating a risk for public health. Conclusion This study shows that the raw milk consumed by the population is contaminated with strains of Enterococcus resistant to antibiotics used in breeding for prophylactic purposes. This requires raising the awareness of those involved in the production and marketing of milk, so as to take measures to apply good hygienic practices and rationalize the use of zootechnical antibiotics.
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- 2018
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36. Synthesis, structural, spectral and antibacterial activity of organic-inorganic compound (C7H9N4O2).[ZnCl3(H2O)] with theophyllinium as Counter Cation
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Aziz Bouymajane, Hicham El Hamdani, and Mohamed El Amane
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010405 organic chemistry ,Hydrogen bond ,Chemistry ,Organic Chemistry ,Infrared spectroscopy ,Tetrahedral molecular geometry ,Protonation ,010402 general chemistry ,01 natural sciences ,0104 chemical sciences ,Analytical Chemistry ,Inorganic Chemistry ,Crystallography ,Proton NMR ,Molecule ,Antibacterial activity ,Spectroscopy ,Monoclinic crystal system - Abstract
Single crystals of organic-inorganic hybrid compound (C7H9N4O2).[ZnCl3(H2O)] has been synthesized by slow evaporation at room temperature and characterized by X-ray diffraction analysis, UV-visible, infrared spectroscopy (FTIR), and 1H NMR. The complex was crystallized in the monoclinic system with P 21/c space group. Moreover, the composition of the complex contains a distorted tetrahedral [ZnCl3(H2O)]−1 anion and one protonated theophyllinium cation. The Zn(II) ion is coordinated by three Cl atoms and one oxygen atom from the water molecule in a close to tetrahedral geometry. The intermolecular hydrogen bonds (O–H…O, O–H…Cl and N-H…Cl) are together playing a vital role in stabilizing of the crystal packing. The antibacterial activity of the complex was evaluated against some bacteria Escherichia coli and Staphylococcus aureus. The compound shown greater antibacterial activity with a MIC value 1.25 mg/µL.
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- 2021
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37. Salvia officinalis and Lippia triphylla: Chemical characterization and evaluation of antidepressant-like activity
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Aziz Bouymajane, Abdelfattah El Moussaoui, Imane Maliki, Hamza Mechchate, Yassine Oulad El Majdoub, Luigi Mondello, Imane Es-Safi, Khalid Elbadaoui, and Francesco Cacciola
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Antidepressant activity ,Aqueous extracts ,Forced swimming test ,Liquid chromatography ,Polyphenols ,Tail suspension test ,Animals ,Antidepressive Agents ,Depression ,Mice ,Plant Extracts ,Swimming ,Lippia ,Salvia officinalis ,Clinical Biochemistry ,Pharmaceutical Science ,01 natural sciences ,Analytical Chemistry ,food ,Drug Discovery ,Imipramine Hydrochloride ,Spectroscopy ,biology ,Traditional medicine ,010405 organic chemistry ,Chemistry ,Verbenaceae ,010401 analytical chemistry ,biology.organism_classification ,food.food ,0104 chemical sciences ,Phytochemical ,Lamiaceae ,Behavioural despair test - Abstract
Salvia officinalis (Lamiaceae) and Lippia triphylla (Verbenaceae) are two plants known for their therapeutic effects in conventional medicine for the treatment of a wide extent of diseases, including the ones on the central nervous system. In the present study, the impact of aqueous extract from the leaves of these two herbs for the treatment of depression was examined. The phytochemical profile highlighted the presence of eighteen and ten polyphenolic compounds in Salvia officinalis and Lippia triphylla, respectively. The antidepressant effects of such extracts were assessed using two tests: the forced swimming test (FST) and tail suspension test (TST) in swiss albino mice. Five mice were partitioned into each group: control (distilled water), standard (Imipramine hydrochloride, 25 mg/kg) and three test ones treated with increased doses of aqueous extracts (250-500-1000 mg/kg), orally administered for two weeks. The acute treatment of the mice with aqueous extracts of Salvia officinalis and Lippia triphylla reduced significantly the time of immobility in the forced swimming test (p < 0.001) as compared to control group, and also decreased significantly the time of immobility of mice in the tail suspension test (p < 0.001). The findings attained in this work show how both plants possess potential anti-depressant-like effects; however, the translation from the results presented in this work to a potential use as therapeutic agents would require the acquisition of a stronger scientific evidence.
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- 2021
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38. Prevalence, characterization and antimicrobial resistance of
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Meryem, Boukili, Fouzia Rhazi, Filali, Nada, Lafkih, Aziz, Bouymajane, Majida, Sefiani, and Mohieddine, Moumni
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Original Article - Abstract
INTRODUCTION: Listeria monocytogenes is one of the most pathogenic bacteria related to the consumption of contaminated food. This study aims to determine the prevalence of L. monocytogenes in raw beef meat in Meknes city of Morocco, to evaluate its pathogenicity and resistance to antimicrobials. METHODS: During four seasons, a total of 140 samples were collected from supermarkets, butcheries and Souk (weekly traditional market). The PCR method was used to examine the presence of specific and virulence genes in the isolated strains, and also to identify their serotypes. The antimicrobial resistance was determined. RESULTS: The results show a prevalence of 7.14% which depends on retail sites and also on the season’s variation. The majority of the strains were detected in butcheries (6 strains), and supermarkets (4 strains). Moreover, the majority of strains were detected during summer (50%). Concerning virulence genes, the seven researched genes were detected in 100% of isolated strains. The majority of strains were of the (1/2a, 1/2c, 3a and 3c) serogroup (70%), while two of them were of the (1/2b, 3b, 4b and 4d) serogroup (20%). All isolates were resistant to at least one antimicrobial, while three strains were resistant to nine tested antimicrobials. However, they were highly susceptible to amikacin, imipenem, gentamicin, sulfamethoxazole and chloramphenicol. CONCLUSIONS: According to results, isolated L. monocytogenes from analyzed beef meat shows a high level of pathogenicity and resistance to the most used antimicrobials in listeriosis therapy, which calls for the severe application of quality systems at the slaughterhouses and retail sites level.
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- 2019
39. Chemical characterization and adsorption of oil mill wastewater on Moroccan clay in order to be used in the agricultural field
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Omar Zennouhi, Laila Nassiri, Aziz Bouymajane, Younes Dehmani, Sadik Abouarnadasse, Abdelaziz Ed-Dra, and Fouzia Rhazi Filali
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Moroccan clay ,0301 basic medicine ,Irrigation ,Fluorescence spectrometry ,Article ,Environmental science ,Biological application ,03 medical and health sciences ,0302 clinical medicine ,Adsorption ,Organic matter ,Dry matter ,lcsh:Social sciences (General) ,lcsh:Science (General) ,Pollutant ,chemistry.chemical_classification ,Multidisciplinary ,Oil mill wastewater ,Pulp and paper industry ,Materials science ,030104 developmental biology ,chemistry ,Wastewater ,Agricultural science ,Germination ,lcsh:H1-99 ,Chemical characterization ,030217 neurology & neurosurgery ,lcsh:Q1-390 - Abstract
Oil mill wastewater (OMW) is the main liquid discharge from oil mills, it is considered as a dangerous pollutant due to its toxic chemical compounds which are unloaded directly in the environment without any treatment. The aims of this study were to evaluate the effectiveness of OMW adsorption on clay as a good method for the elimination of toxic chemical compounds and to study the application of treated OMW as an irrigation source in agricultural field. For this, Clay was collected from the city of Agourai (Meknes region, Morocco) and characterized by X-ray diffraction, X-ray fluorescence spectrometry, BET and FTIR analysis. Moreover, the treated OMW was analyzed using UHPLC-ESI-MS and the determination of total phenolic content (TPC) was also performed. However, the application of the treated OMW in agricultural field was performed by the determination of its effect on the germination of Lepidium sativum seeds (in vitro) and as a source of irrigation of Vicia faba plants (in situ). The results of this study showed that OMW had the following physicochemical characteristics: average pH of 4.88, TPC of 4.75 g/l, COD of 80 g/l, BOD5 of 18.72 g/l, conductivity of 16.05 cm-1, dry matter of 135.7 g/l and volatile matter of 58.7 g/l. The adsorption on clay had increased the pH from 4.88 to 6.14 and reduced significantly the organic matter (42% of COD and 57.4% of phenolic compounds). UHPLC-ESI-MS analysis showed the presence of a wide variety of organic compounds in OMW, with the appearance of new compounds after adsorption. Moreover, the use of treated OMW as a source of irrigation showed a significant effect on the germination of Lepidium sativum seeds and the growth of Vicia faba plants. From this study, we can conclude that the adsorption on clay is a good method for the treatment of OMW, which became non-toxic for environment and can be used as a source of irrigation in agricultural field., Agricultural science; Environmental science; Materials science, Oil mill wastewater; Adsorption; Moroccan clay; Chemical characterization; Biological application.
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- 2020
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40. Application of Mentha suaveolens essential oil as an antimicrobial agent in fresh turkey sausages
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Mohamed Bou-Idra, Najia Moukrad, Faouzia Benhallam, Amar Bentayeb, Abdelaziz Ed-Dra, Fouzia Rhazi Filai, Aziz Bouymajane, and Badr Zekkori
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Traditional medicine ,Mentha suaveolens ,Food preservation ,Plant Science ,Biology ,Antimicrobial ,General Biochemistry, Genetics and Molecular Biology ,food.food ,law.invention ,food ,law ,Antibacterial activity ,Agronomy and Crop Science ,Essential oil ,Food Science - Published
- 2018
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41. Occurrence, molecular and antimicrobial resistance of
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Aziz, Bouymajane, Fouzia, Rhazi Filali, Said, Oulghazi, Abdelaziz, Ed-Dra, Faouzia, Benhallam, Abdallah, El Allaoui, Jaouad, Anissi, Khalid, Sendide, Bouchra, Ouhmidou, and Mohieddine, Moumni
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During the period of May 2015 through April 2016, 150 cow's raw milk samples were collected from street traders in Meknes city. They were examined for the identification ofThe results showed that 11.3% (17/150) of samples were positive for the presence ofThis study shows that the raw milk consumed by the population is contaminated with strains of
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- 2017
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