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Introduction: The aim of the present work is to perform dosimetric characterization of a novel vented PinPoint ionization chamber (PTW 31023, PTW-Freiburg, Germany). This chamber replaces the previous model (PTW 31014), where the diameter of the central electrode has been increased from 0.3 to 0.6mm and the guard ring has been redesigned. Correction factors for reference and non-reference measurement conditions were examined., Materials and Methods: Measurements and calculations of the correction factors were performed according to the DIN 6800-2. The shifts of the effective point of measurement (EPOM) from the chamber's reference point were determined by comparison of the measured PDD with the reference curve obtained with a Roos chamber. Its lateral dose response functions, which act according to a mathematical convolution model as the convolution kernel transforming the dose profile D(x) to the measured signal M(x), have been approximated by Gaussian functions with standard deviation σ. Additionally, the saturation correction factors k S have been determined using different dose-per-pulse (DPP) values. The polarity effect correction factors k P were measured for field sizes from 5cm×5cm to 40cm×40cm. The influence of the diameter of the central electrode and the new guard ring on the beam quality correction factors k Q was studied by Monte-Carlo simulations. The non-reference condition correction factors k NR have been computed for 6MV photo beam by varying the field size and measurement depth. Comparisons on these aspects have been made to the previous model., Results: The shifts of the EPOM from the reference point, Δz, are found to be -0.55 (6MV) and -0.56 (10MV) in the radial orientation and -0.97mm (6MV) and -0.91mm (10MV) in the axial orientation. All values of Δz have an uncertainty of 0.1mm. The σ values are 0.80mm (axial), 0.75mm (radial lateral) and 1.76mm (radial longitudinal) for 6MV photon beam and are 0.85mm (axial), 0.75mm (radial lateral) and 1.82mm (radial longitudinal) for 15MV photon beam. All σ values have an uncertainty of 0.05mm. The correction factor k S was found to be 1.0034±0.0009 for the PTW 31014 chamber and 1.0024±0.0007 for the PTW 31023 chamber at the highest DPP (0.827mGy) investigated in this study. Under reference conditions, the polarity effect correction factor k P of the PTW 31014 chamber is 1.0094 and 1.0116 for 6 and 10MV respectively, while the k P of the PTW 31023 chamber is 1.0005 and 1.0013 for 6 and 10MV respectively, all values have an uncertainty of 0.002. The k P of the new chamber also exhibits a weaker field size dependence. The k Q values of the PTW 31023 chamber are closer to unity than those of the PTW 31014 chamber due to the thicker central electrode and the new guard ring design. The k NR values of the PTW 31023 chamber for 6MV photon beam deviate by not more than 1% from unity for the conditions investigated., Discussions: Correction factors associated with the new chamber required to perform reference and relative dose measurements have been determined according to the DIN-protocol. The correction factor k S of the new chamber is 0.1% smaller than that of the PTW 31014 at the highest DPP investigated. Under reference conditions, the correction factor k P of the PTW 31023 chamber is approximately 1% smaller than that of the PTW 31014 chamber for both energies used. The dosimetric characteristics of the new chamber investigated in this work have been demonstrated to fulfil the requirements of the TG-51 addendum for reference-class dosimeters at reference conditions., (Copyright © 2019. Published by Elsevier GmbH.)

Purpose: Discrepancy between experimental and Monte Carlo simulated dose-response of the microDiamond (mD) detector (type 60019, PTW Freiburg, Germany) at small field sizes has been reported. In this work, the radiation-induced charge imbalance in the structural components of the detector has been investigated as the possible cause of this discrepancy., Materials and Methods: Output ratio (OR) measurements have been performed using standard and modified versions of the mD detector at nominal field sizes from 6 mm × 6 mm to 40 mm × 40 mm. In the first modified mD detector (mD_reversed), the type of charge carriers collected is reversed by connecting the opposite contact to the electrometer. In the second modified mD detector (mD_shortened), the detector's contacts have been shortened. The third modified mD detector (mD_noChip) is the same as the standard version but the diamond chip with the sensitive volume has been removed. Output correction factors were calculated from the measured OR and simulated using the EGSnrc package. An adapted Monte Carlo user-code has been used to study the underlying mechanisms of the field size-dependent charge imbalance and to identify the detector's structural components contributing to this effect., Results: At the smallest field size investigated, the OR measured using the standard mD detector is >3% higher than the OR obtained using the modified mD detector with reversed contact (mD_reversed). Combining the results obtained with the different versions of the detector, the OR have been corrected for the effect of radiation imbalance. The OR obtained using the modified mD detector with shortened contacts (mD_shortened) agree with the corrected OR, all showing an over-response of less than 2% at the field sizes investigated. The discrepancy between the experimental and simulated output correction factors has been eliminated after accounting for the effect of charge imbalance., Discussions and Conclusions: The role of radiation-induced charge imbalance on the dose-response of mD detector in small field dosimetry has been studied and quantified. It has been demonstrated that the effect is significant at small field sizes. Multiple methods were used to quantify the effect of charge imbalance with good agreement between Monte Carlo simulations and experimental results obtained with modified detectors. When this correction is applied to the Monte Carlo data, the discrepancy from experimental data is eliminated. Based on the detailed component analysis using an adapted Monte Carlo user-code, it has been demonstrated that the effect of charge imbalance can be minimized by modifying the design of the detector's contacts., (© 2019 The Authors. Medical Physics published by Wiley Periodicals, Inc. on behalf of American Association of Physicists in Medicine.)

Purpose: The recent developments of compact air-filled ionization chambers for use in small photon beams have raised the needs to address the associated polarity effect. The polarity effect of five compact ionization chambers has been quantified at small field sizes. The origins of the polarity effect are studied experimentally and through Monte-Carlo simulations. For this purpose, the one-dimensional lateral dose-response functions were determined using positive and negative chamber's polarity. Monte-Carlo simulations were performed to study the underlying mechanism of the polarity effect by quantifying the charge imbalance in the collecting electrode and cable., Methods: Five novel compact ionization chamber designs have been studied (PTW-Freiburg: Semiflex 3D 31021, PinPoint 3D 31022 and PinPoint 31023; IBA Dosimetry: Razor chamber CC01-G and Razor Nano-chamber CC003). Output ratios were measured down to a nominal field side length of 3 mm using both polarities to evaluate the polarity effect at different field sizes. The small field output correction factors were derived using a scintillator detector as reference. To identify the origins of the polarity effect, slit beam measurements were performed to obtain their lateral dose-response functions. All measurements were performed using three chamber orientations: axial, radial crossplane, and radial inplane. The chambers were modeled according to the manufacturers' blueprints using the Monte-Carlo package EGSnrc. The charge imbalance due to electrons entering and leaving the inner electrode and cable was studied using an adapted user-code., Results: The output ratios obtained using all five chambers show field size-dependent polarity effects at small field sizes in the axial orientation, whereas no significant field size dependence of the polarity effect has been observed in the radial orientations. The chambers' lateral dose-response functions reveal that the radiation-induced charge imbalance in the inner electrode and cable is the main cause of the observed polarity effect at small field sizes. The effect is weakest for the largest PTW 31021 chamber but intensifies for smaller chambers with decreasing sensitive air volume. Consistent results have been obtained between Monte-Carlo simulations and measurement data., Conclusions: Awareness needs to be raised so that the polarity effect of novel compact ionization chambers is appropriately accounted for in small field dosimetry. The results in this work are useful to identify the magnitude of the polarity effect correction and to assist in the decisions on choosing the appropriate chambers and setups during measurements. The origins of the observed polarity effect have been elucidated using the chambers' lateral dose-response functions. The adapted Monte-Carlo user-code has been used to compute the radiation-induced charge imbalance in the chamber's components. It opens the possibility to study the chamber's design with the aim to minimize its polarity effect. Small field output correction factors computed according to TRS 483 have been reported for these investigated chambers., (© 2018 American Association of Physicists in Medicine.)

Background: The denitrifying betaproteobacterium "Aromatoleum aromaticum" EbN1 anaerobically utilizes a multitude of aromatic compounds via specific peripheral degradation routes. Compound-specific formation of these catabolic modules is assumed to be mediated by specific transcriptional activators. In case of the recently elucidated p-ethylphenol/p-hydroxyacetophenone pathway, the highly substrate-specific regulation was implicated to involve the predicted σ(54)-dependent, NtrC-type regulator EbA324. The latter was suggested to control the expression of the two neighboring gene clusters encoding the catabolic enzymes as well as a corresponding putative solvent efflux system. In the present study, a molecular genetic approach was used to study the predicted function of EbA324., Results: An unmarked in frame ΔebA324 (here renamed as ΔetpR; p-ethylphenol regulator) deletion mutation was generated. The ΔetpR mutant was unable to grow anaerobically with either p-ethylphenol or p-hydroxyacetophenone. Growth similar to the wild type was restored in the ΔetpR mutant background by in trans expression of plasmid-born etpR. Furthermore, expression of the "p-ethylphenol" gene clusters as well as corresponding protein formation was shown to depend on the presence of both, EtpR and either p-ethylphenol or p-hydroxyacetophenone. In the wild type, the etpR gene appears to be constitutively expressed and its expression level not to be modulated upon effector presence. Comparison with the regulatory domains of known phenol- and alkylbenzene-responsive NtrC-type regulators of Pseudomonas spp. and Thauera aromatica allowed identifying >60 amino acid residues in the regulatory domain (in particular positions 149 to 192 of EtpR) that may contribute to the effector specificity viz. presumptively restricted effector spectrum of EtpR., Conclusions: This study provides experimental evidence for the genome predicted σ(54)-dependent regulator EtpR (formerly EbA324) of "A. aromaticum" EbN1 to be responsive to p-ethylphenol, as well as its degradation intermediate p-hydroxyacetophenone, and to control the expression of genes involved in the anaerobic degradation of these two aromatic growth substrates. Overall, the presented results advance our understanding on the regulation of anaerobic aromatic compound catabolism, foremost based on the sensory discrimination of structurally similar substrates.

The dehydrogenation of 1-(4-hydroxyphenyl)-ethanol to 4-hydroxyacetophenone represents the second reaction step during anaerobic degradation of p-ethylphenol in the denitrifying bacterium 'Aromatoleum aromaticum' EbN1. Previous proteogenomic studies identified two different proteins (ChnA and EbA309) as possible candidates for catalyzing this reaction [Wöhlbrand et al: J Bacteriol 2008;190:5699-5709]. Physiological-molecular characterization of newly generated unmarked in-frame deletion and complementation mutants allowed defining ChnA (renamed here as Hped) as the enzyme responsible for 1-(4-hydroxyphenyl)-ethanol oxidation. Hped [1-(4-hydroxyphenyl)-ethanol dehydrogenase] belongs to the 'classical' family within the short-chain alcohol dehydrogenase/reductase (SDR) superfamily. Hped was overproduced in Escherichia coli, purified and crystallized. The X-ray structures of the apo- and NAD(+)-soaked form were resolved at 1.5 and 1.1 Å, respectively, and revealed Hped as a typical homotetrameric SDR. Modeling of the substrate 4-hydroxyacetophenone (reductive direction of Hped) into the active site revealed the structural determinants of the strict (R)-specificity of Hped (Phe(187)), contrasting the (S)-specificity of previously reported 1-phenylethanol dehydrogenase (Ped; Tyr(93)) from strain EbN1 [Höffken et al: Biochemistry 2006;45:82-93]., (© 2015 S. Karger AG, Basel.)