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To finish this special issue, some friends, colleagues and students of Prof. Chow (Emeritus Professor, the Research School of Biology, the Australian National University) have written small tributes to acknowledge not only his eminent career but to describe his wonderful personality.

Dropshipping is a sales business model in which a seller, called a drop shipper, promotes goods sold by a supplier. If there is an order, the drop shipper directly orders the supplier. Suppliers will process orders and send orders to buyers on behalf of the drop shipper. Dropshipping is popular among people in the Indonesian marketplace. Some marketplace even supports dropshipping features for their drop shipper. Therefore, this paper proposes Naïve Bayes method predict whether a seller is a drop shipper or not. This research is part of recommendation system research goal. Since there is a drop shipping mechanism in the marketplace, there is a stock problem. This research is essential because by classifying a seller as a drop shipper or not, we can draw a stock of a product that the buyer searches for by eliminating stock of drop shippers and predicting stock from the supplier in the marketplace. By solving stock issues, we can afford better recommendations for a buyer. There are two scenarios for testing: 1) fixed datasets. In scenario 1) KNN has 88.61% accuracy for shopee dataset and 85.42% accuracy for tokopedia dataset. Naïve Bayes has 86.67% in bukalapak dataset; 2) growth datasets. In first until third testing accuracy of the Naïve Bayes method is 80%, and increased 1% (81.1%) using all of the data in the shopee dataset. In tokopedia dataset, Naïve Bayes shows a good trend line in three scenarios but down in the last data. Accuracy of Naïve Bayes decreased 1.06% from third testing to all data. Last in bukalapak dataset, Naïve Bayes leads the performance with 9% accuracy increasing from 87.50 to 96.

GANIL Facilities deliver ion beams from Proton to Uranium up to 95MeV/A. The Cyclotron facility combined with the SPIRAL1 facility provides Stable Ion Beams (SIB) since 1983 and Radioactive Ion Beams (RIB) since 2001 for Physics experiments. R&D of new stable and radioactive beams are continuously under progress matching physicist requirements. During the last ten years, the SPIRAL1 facility was upgraded to extend its capabilities to deliver RIBs of condensable elements. After a new one delivered in 2019, off-line R&D’s have been done on the Target Ions Source System and also on the Charge Breeder to improve efficiencies and achieve an operational and reliable facility. In parallel, the commissioning of the LINAC-SPIRAL2 facility started in 2019 with a proton beam production. In 2020, a 16 kW proton beam was delivered for the first time on the Neutron For Science physics area, and a 4 mA beam of 4He2+ was produced with the PhoenixV3 ECR ion source to mimic a deuteron beam. In this article, an overview of GANIL ion sources, beam properties and future developments are described.

A new resistive oven dedicated to the production of metal ion beams using ECR ion sources has been developed at GANIL. It aims to produce beams such as 58Niq+, 50Tiq+, 50Vq+ or 238Uq+ with ECR4 ion source on cyclotrons injectors and with Phoenix V3 ion source on Spiral 2facility. Typical intensity about 1.2 1013 pps is requested for the Super Separator Spectrometer (S3). Evaporated atom fluxes and their angular distributions have been measured on a dedicated test bench thanks to a quartz microbalance. These investigations were carried out using three crucibles with various exit caps geometries for which the fluxes and angular distributions display distinct behaviors. Based on those results, crucible is designed to obtain a high flux of atoms overlapping the ECR plasma while minimizing the atoms losses on the ion source chamber.

Induction of non-photochemical quenching (NPQ) of chlorophyll fluorescence in leaves affords photoprotection to the photosynthetic apparatus when, for whatever reason, photon capture in the antennae of photosystems exceeds their capacity to utilise this excitation in photochemistry and ultimately in CO2 assimilation. Here we augment traditional monitoring of NPQ using the fast time resolution, remote and relatively non-intrusive light induced fluorescence transient (LIFT) technique (Kolber et al . 2005 ; Osmond et al . 2017 ) that allows direct measurement of functional (σ'PSII ) and optical cross-sections (a 'PSII ) of PSII in situ , and calculates the half saturation light intensity for ETR (E k ). These parameters are obtained from the saturation and relaxation phases of fluorescence transients elicited by a sequence of 270, high intensity 1 μs flashlets at controlled time intervals over a period of 30 ms in the QA flash at intervals of a few seconds. We report that although σ'PSII undergoes large transient increases after transfer from dark to strong white light (WL) it declines little in steady-state as NPQ is induced in shade- and sun-grown spinach and Arabidopsis genotypes Col , OEpsbs , pgr 5bkg , stn 7 and stn 7/8. In contrast, σ'PSII increases by ~30% when induction of NPQ in spinach is inhibited by dithiothreitol and by inhibition of NPQ in Arabidopsis npq 1, npq 4 and pgr 5. We propose this increase in σ'PSII arises as some excitation from closed PSII reaction centres is transferred to open centres when excitation partitioning to photochemistry (Y II ) and NPQ (Y NP ) declines, and is indicated by an increased excitation dissipation from closed PSII centres (Y NO , including fluorescence emission). Although E k increases following dissipation of excitation as heat when NPQ is engaged, it declines when NPQ is inhibited. Evidently photochemistry becomes more easily light saturated when excitation is transferred from closed RCIIs to open centres with larger σ'PSII . The NPQ mutant pgr 5 is an exception; E k increases markedly in strong light as electron transport QA → PQ and PQ → PSI accelerate and the PQ pool becomes strongly reduced. These novel in situ observations are discussed in the context of contemporary evidence for functional and structural changes in the photosynthetic apparatus during induction of NPQ.

The design, realization and operation of a prototype or “demonstrator” version of an active target and time projection chamber (ACTAR TPC) for experiments in nuclear physics is presented in detail. The heart of the detection system features a micromegas gas amplifier coupled to a high-density pixelated pad plane with square pad sizes of 2 × 2 mm 2 . The detector has been thoroughly tested with several different gas mixtures over a wide range of pressures and using a variety of sources of ionizing radiation including laser light, an α -particle source and heavy-ion beams of 24Mg and 58Ni accelerated to energies of 4.0 MeV/u. Results from these tests and characterization of the detector response over a wide range of operating conditions will be described. These developments have served as the basis for the design of a larger detection system that is presently under construction.

International audience; The 1+/n+ method, based on an ECRIS charge breeder (CB) originally developed at the LPSC laboratory, is now implemented at GANIL for the production of Radioactive Ion Beams (RIBs). Prior to its installation in the middle of the low energy beam line of the SPIRAL1 facility, the 1+/n+ system CB has been modified based on the experiments performed on the CARIBU Facility at Argone National Laboratory. Later, it has been tested at the 1+/n+ LPSC test bench to validate its operation performances. Charge breeding efficiencies as well as charge breeding times have been measured for noble gases and alkali elements. The commissioning phase started at GANIL in the second half-year of 2017. It consisted of a stepwise process to test the upgrade of the SPIRAL1 facility from simple validation [operation of Charge Breeder (CB) as a stand-alone source] up to the production of the first 1+/n+ RIB. Thus, this year, a 38mK/38K RIB has been successfully delivered to a physics experiment over a period of 1 week. The yields on the physics target were in the range of ∼2–4 × 106 pps at 9 MeV/u. The target ion source system (TISS) was made of a FEBIAD ion source connected to a hot graphite target. This is the first time a RIB is accelerated with a cyclotron with the 1+/n+ method. Moreover, a production test with the FEBIAD TISS has confirmed the yields measured previously, which validates the extension of the GANIL/SPIRAL1 catalog for a number of isotopes. In parallel, R&D is being performed on new TISSs (e.g., a fast release one, using surface ionization source). Targets are also a subject of ongoing R&D for yield and release time optimization. This contribution will present the new acceleration scheme of the SPIRAL1 facility, which largely extends the palette of RIBs available for nuclear physicists. It will be compared to the ones used at similar ISOL facilities. This facility is more than a simple ISOL facility, and an overview of the new opportunities offered by the upgraded installation will be also discussed.

Since 2001, the SPIRAL 1 facility has been one of the pioneering facilities in ISOL techniques for reaccelerating radioactive ion beams: the fragmentation of the heavy ion beams of GANIL on graphite targets and subsequent ionization in the Nanogan ECR ion source has permitted to deliver beams of gaseous elements (He, N, O, F, Ne, Ar, Kr) to numerous experiments. Thanks to the CIME cyclotron, energies up to 20 AMeV could be obtained. In 2014, the facility was stopped to undertake a major upgrade, with the aim to extend the production capabilities of SPIRAL 1 to a number of new elements. This upgrade, which is presently under commissioning, consists in the integration of an ECR booster in the SPIRAL 1 beam line to charge breed the beam of different 1+ sources. A FEBIAD source (the so-called VADIS from ISOLDE) was chosen to be the future workhorse for producing many metallic ion beams. The charge breeder is an upgraded version of the Phoenix booster which was previously tested in ISOLDE. The performances of the aforementioned ingredients of the upgrade (targets, 1+ source and charge breeder) have been and are still being optimized in the frame of different European projects (EMILIE, ENSAR and ENSAR2). The upgraded SPIRAL 1 facility will provide soon its first new beams for physics and further beam development are undertaken to prepare for the next AGATA campaign. The results obtained during the on-line commissioning period permit to evaluate intensities for new beams from the upgraded facility., 9 pages, 12 figures, conference proceedings of EMIS 2018, CERN, Geneva, to appear in Nuclear Instruments and Methods B

International audience; SPIRAL1 Upgrade hardware is now almost completed. The FEBIAD 1+ source has been tested for the production of new radioactive isotopes, the SPIRAL1 Charge Breeder (SP1 CB) is in place reproducing nearly the charge breeding efficiencies measured at LPSC laboratory and the infrastructure is operational. The commissioning phase started in the first semester of 2017. It has consisted of a stepwise process to test the upgrade of the SPIRAL1 facility from simple validation (operation of SP1 CB as a stand-alone source) up to the production of the first 1+/N+ Radioactive Ion Beam (RIB) with the 37K9+ ion. This contribution will summarize the different steps completed successfully and especially the measurements performed to validate each of the commissioning stages. These include e.g. ionization efficiency measurements for CB; beam line optics for 1+/N+ and charge breeding tuning. The remaining effort required to ensure the reliability of the complete system for routine RIB operation is also presented. A section will be dedicated to the coupling of SP1 CB to the CIME cyclotron, leading to the delivery of stable beams at unprecedented energies at GANIL.

International audience; The 1+/n+ method, originally developed at the Laboratoire de Physique Subatomique & Cosmology (LPSC) laboratory, is implemented at GANIL for the production of radioactive ion beams as well as for studying the production of intense stable metallic beams. Regarding radioactive ion beam production, the ECRIS type Phoenix charge breeder of the Système de Production d’Ions Radioactifs Accélérés en Ligne (SPIRAL1) has been installed in the midst of the SPIRAL1 Low Energy Beam Transport (LEBT). First beam of 40Arn+ was extracted during May 2017 demonstrating a distribution peaked on the 9+ charge state with 16% in particle of ionization efficiency. Concerning stable ion beam production, the Grenoble Test Source (GTS) ECR ion source, providing stable ion beam for the Accélérateur pour les Recherches Interdisciplinaires avec les Ions de Basse Energie (ARIBE) facility, is under modification to include a charge breeding capability to study a new injection process for producing high intensity metallic beams.

Laser ion sources are in use extensively to ionize the nuclear reaction products at many on-line radioactive ion beam facilities. They have proven to be reliable and to combine good production efficiencies with chemical selectivity. A Laser ion source is currently under development for the SPIRAL2 project at GANIL. A status update of this project called GISELE is presented.

International audience; The Resonance Ionization Laser Ion Source (RILIS) has emerged as an important technique in many Radioactive Ion Beam (RIB) facilities for its reliability, and ability to ionize target elements efficiently and element selectively. GISELE is an off-line RILIS test bench to study the implementation of an on-line laser ion source at the GANIL separator facility. The aim of this project is to determine the best technical solution which combines high selectivity and ionization efficiency with small ion beam emittance and stable long term operation. The ion source geometry was tested in several configurations in order to find a solution with optimal ionization efficiency and beam emittance. Furthermore, a low work function material was tested to reduce the contaminants and molecular sidebands generated inside the ion source. First results with ZrC ionizer tubes will be presented. Furthermore, a method to measure the energy distribution of the ion beam as a function of the time of flight will be discussed.

Joan Mary Anderson, known to most people as Jan, was born on May 12, 1932 in Dunedin, New Zealand. She died on August 28, 2015 in Canberra, Australia. To celebrate her life, we present here a brief biography, some comments on her discoveries in photosynthesis during a career spanning more than half a century, and reminiscences from family and friends. We remember this wonderful person who had an unflagging curiosity, creative ability to think laterally, enthusiasm, passion, generosity and love of color and culture.

ThuPE31; International audience; In the framework of the SPIRAL1 upgrade under progress at the GANIL lab, the charge breederbased on a LPSC Phoenix ECRIS, first tested at ISOLDE has been modified to benefit of thelast enhancements of this device from the 1+/n+ community. The modifications mainly concernthe 1+ optics, vacuum techniques, and the RF—buffer gas injection into the charge breeder. Priorto its installation in the midst of the low energy beam line of the SPIRAL1 facility, it has beendecided to qualify its performances and several operation modes at the test bench of LPSC lab. Thiscontribution shall present preliminary results of experiments conducted at LPSC concerning the 1+to n+ conversion efficiencies for noble gases as well as for alkali elements and the correspondingtransformation times.

SUMMARY The extent to which studies of photosynthesis and carbon economy are, or should be, at the forefront of comparative plant ecology is discussed from the points of view of carbon pathways, water-use efficiency, irradiance and nutrients, especially nitrogen. The ways in which new integrative and reductionist techniques can be applied to photosynthesis in an ecological control are illustrated and their value assessed. The study of photosynthetic physiology of plants at the edges of their geographical range is urged as a means of understanding both their responses to stress and disturbance, and their competitive interactions.

TuePS05; International audience; Resonant Ionization Laser Ion Source (RILIS) is nowadays an important technique in many RadioactiveIon Beam (RIB) facilities for its reliability and ability to ionize efficiently and element selectively.Grand Accélérateur National d’Ions Lourds (GANIL) Ion Source using Electron Laser Excitation(GISELE) is an off-line test bench for RILIS developed to study a fully operational resonant laserion source at GANIL facility. The ion source body has been designed as a modular system toinvestigate different experimental approaches by varying the design parameters, to develop the futureon-line laser ion source. The aim of this project is to determine the best technical solution whichcombines high selectivity and ionization efficiency with small ion beam emittance and stable longterm operation. Latest results concerning emittance and time profile development as a functionof the temperature for different ion source versions will be presented.

Infection with phloem limited Abutilon Mosaic Virus caused localized carbohydrate accumulation (high levels of starch, sucrose, and hexoses) in leaves of Abutilon striatum during early symptom development. In mature leaves with attenuated symptoms, tissues showing faint vein-clearing had markedly higher carbohydrate contents than uniformly green areas of the same leaf. A similar pattern of carbohydrate accumulation was found in pale-green mosaics in mature leaves with overt symptoms when compared to green-islands of the same leaf, but overnight carbohydrate loses were comparable to controls. Because leaves with attenuated symptoms showed no further symptom development whereas the pale-green mosaics became yellow and eventually necrotic in leaves with overt symptoms, it seems unlikely that carbohydrate accumulation following impaired translocation was responsible for symptom expression. High carbohydrate status in leaves with attenuated symptoms had little effect on nonphotochemical quenching during early stages of photosynthetic induction. In leaves with overt symptoms, areas of high carbohydrate status with pale-green mosaics showed markedly slower nonphotochemical quenching. Early symptom areas of young leaves, and advanced symptom areas of mature leaves had low starch contents but were otherwise similar to controls in carbohydrate status. Impaired nonphotochemical quenching in these tissues tended to reflect the state of symptom development, rather than carbohydrate status. Plants with overt symptoms grew about half as fast as plants with attenuated symptoms.

After exposure to full sunlight under natural conditions, attached leaves of the common meadow weed Potentilla reptans show non-uniform (“patchy”) chlorophyll fluorescence quenching in the early stages of fluorescence transients. These areas of bright fluorescence can be readily reproduced in detached leaves that are allowed to wilt on the laboratory bench in weak light. The extent and duration of the patchiness increases with increasing water stress (higher relative saturation deficits). Images captured during saturating flashes show that the patches also display slow development of non-photochemical quenching, consistent with the possibility that photosynthetic metabolism is impaired in these areas. Wilted Potentilla leaves readily regain full turgor when petioles are placed in water, and uniform chlorophyll fluorescence is recovered with in 30mm. Epidermal impressions reveal closed stomata over areas of both low and high fluorescence in wilted leaves. Because highly fluorescent patches also persist when wilted tissues are exposed to high CO2 (i.e., patchiness is unlikely to be due to local differences in CO2 supply) the data suggest direct effects of water stress on metabolism in wilted leaves. Leaf transverse sections show that although major veins may isolate areas of the lamina, minor veins do not. Relationships to leaf anatomy are discussed.

Leaves of Stephania japonica and Smilax australis were characterized in situ on the coast of north-eastern New South Wales, Australia, where they were growing naturally in three different light environments: deep shade, in the understory of an open Eucalyptus forest where they received frequent sunflecks of high intensity, and in an exposed site receiving full sunlight. In deep shade the xanthophyll cycle remained epoxidized during the day and the vast majority of absorbed light was utilized for photosynthesis. In the exposed site both deepoxidation and epoxidation of the xanthophyll cycle and changes in the level of xanthophyll-dependent thermal energy dissipation largely tracked the diurnal changes in photon flux density (PFD). In the understory the xanthophyll cycle became largely deepoxidized to zeaxanthin and antheraxanthin upon exposure of the leaves to the first high intensity sunfleck and this high level of deepoxidation was maintained throughout the day both during and between subsequent sunflecks. In contrast, thermal energy dissipation activity, and the efficiency of photosystem II, fluctuated rapidly in response to the changes in incident PFD. These findings suggest a fine level of control over the engagement of zeaxanthin and antheraxanthin in energy dissipation activity, presumably through rapid changes in thylakoid acidification, such that they became rapidly engaged for photoprotection during the sunflecks and rapidly disengaged upon return to low light when continued engagement might limit carbon gain.

A portable Chi fluorescence imaging system was used to characterise nonuniform Chi fluorescence quenching in Abutilon striatum leaves infected with phloem-localised abutilon mosaic virus. The instrument was used to observe fluorescence emission at intervals during induction transients, and to map nonphotochemical quenching during saturating pulses applied in the course of these transients. Two symptom types were distinguished: yellow vein-associated motifs that showed lower maximum Chi fluorescence than nearby green tissues, but virtually zero nonphotochemical quenching, and vein-defined mosaics (pale green) that initially showed normal maximum Chi fluorescence but strongly impaired nonphotochemical quenching. Mature vein-defined mosaics (yellow to white areas) resembled vein-associated symptoms with zero nonphotochemical quenching. Islands of apparently healthy green tissue enclosed by mosaic symptoms showed slower nonphotochemical quenching than controls. Possible effects of localised carbohydrate accumulation, thought to follow from infection by the phloem-limited virus, on photosynthetic processes as well as the synthesis and stability of chloroplast protein complexes, are discussed in the context of symptom development.

The effects of introduced chloroplast gene mutations affecting D1 synthesis, turnover and function on photosynthesis, growth and competitive ability were examined in autotrophic cultures of Chlamydomonas reinhardtii (Chlorophyta) adapted to low or high irradiance. Few discernible effects were evident when the mutants were grown in low light (LL, 70 μmol m−2 s−1). The herbicide-resistant psbA mutation Ser264→ Ala (dr) slowed electron transfer and accelerated D1 degradation in cells grown under high light (HL, 600 μmol m−2 s−1). The maximum rate of light-and CO2-saturated photosynthesis, cell growth rate and competitive ability in the dr mutant were reduced compared to wild type under HL. However, the wild-type rate of D1 synthesis in dr was adequate to compensate for accelerated D1 degradation. 16S rRNA mutations conferring resistance to streptomycin and spectinomycin (spr/sr) that altered chloroplast ribosome structure and assembly were used to inhibit chloroplast protein synthesis. In spr/sr cells grown under HL, D1 synthesis was reduced by 40–60% compared to wild type and D1 degradation was accelerated, leading to a 4-fold reduction in D1 pool size. The reduced D1 levels were accompanied by an elevation of Fo and a decline in Fv/Fm, quantum yield and maximum rate of CO2-saturated photosynthesis. Chemostat experiments showed that the growth rate and competitive ability of spr/sr were reduced against both wild type and dr.

The consequences of biotic stress have been poorly understood, partly because its application is difficult to control and partly because its physiological consequences are highly variable. Many plant viruses are recognised on the basis of leaf symptoms that depend on localised changes to chloroplast structure and function. This paper reviews recent progress in understanding early interactions between plant viruses and the photosynthetic apparatus, using chlorophyll fluorescence analysis of novel, defined algal-virus systems and using high resolution imaging of chlorophyll fluorescence and other photosynthetic processes in higher plant systems. We then consider the consequences of viral effects on photosynthetic functioning for whole plants and populations with an emphasis on the potential interactions with other environmental factors. Early responses indicated by increase in both non-photochemical quenching of fluorescence and increased reduction state of the primary electron transport acceptor QA suggest that, not surprisingly, both photoprotective and photoinhibitory processes contribute to the accelerated local demise of the photosynthetic apparatus and symptom development. In other cases, localised accumulations of carbohydrate and source-sink imbalance following infection may inhibit gene expression, leading to altered levels of chloroplast protein complexes and enzymes of photosynthetic metabolism coincident with symptom development. Recent experiments suggest that much of the variability in plant responses to biotic stress may result from interactions with other environmental factors, such as light intensity and nutrition. Experiments suggest that virus infections may have greater effects on fitness and competitive ability in low N, high light environments than in shaded, high nutrient conditions. Some ecological implications of these observations are discussed.

The SPIRAL 2 facility, currently under construction, will provide either stable or radioactive beams at high intensity. In addition to the high intensity of stable beams, high charge states must be produced by the ion source to fulfill the RFQ LINAC injection requirements: Q/A = 1/3 at 60 kV ion source extraction voltage. Excepting deuterons and hydrogen, most of the stable beam requests concern metallic elements. The existing 18 GHz electron cyclotron resonance ion source (ECRIS) Phoenix V2 designed at LPSC Grenoble has been used for the tests and will be the source for the SPIRAL 2 commissioning. The tests performed at LPSC for calcium ((40)Ca(14+) and (40)Ca(16+)), nickel ((58)Ni(19+)), and sulfur ((32)S(11+)) are described and discussed. Due to the very high charge states required, the oven method has been chosen. An intensity of 1 pμA has been reached for those elements. The performance and the beam stability have been studied using different buffer gases, and some ionization efficiency preliminary results are given.

Freezing and thawing of the endemic moss species Grimmia antarctici Card, caused photoinhibition. When snow cover was removed from moss in the field, resulting in exposure to fluctuating temperatures and light conditions, photoinhibition, measured as a reduction in the ratio of variable to maximum chlorophyll a fluorescence (F-v/F-m), was observed. The extent of photoinhibition was highly variable and appeared to be reversible during periods of warmer temperatures, A series of controlled laboratory studies found that the light conditions that prevail between freezing and thawing events influenced the recovery from photoinhibition observed during freezing and thawing, with low light conditions facilitating the greatest rates of recovery, After four cycles of freezing and thawing, recovery from photoinhibition in hydrated moss was achieved within 12 h of transfer to 5 degrees C and 15 mu mol quanta m(-2) s(-1). These results favour the hypothesis that photoinhibition observed during freezing represents a protective process involving the down-regulation of photosystem II when photosynthetic carbon assimilation is limited by low temperatures.

Arabidopsis thaliana leaves were examined in short-term (1 h) and long-term (10 h) irradiance experiments involving growth, saturating and excess light. Changes in photosynthetic and chlorophyll fluorescence parameters and in populations of functional photosystem II (PSII) centers were independently measured. Xanthophyll pigments, 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU)-binding sites, the amounts of D1 protein, and the rates of D1 protein synthesis were determined. These comprehensive studies revealed that under growth or light-saturating conditions, photosynthetic parameters remained largely unaltered. Photoprotection occurred at light saturation indicated by a dark-reversible increase in non-photochemical quenching accompanied by a 5-fold increase in antheraxanthin and zeaxanthin. No consistent change in the concentrations of functional PSII centers, DCMU-binding sites, or D1 protein pool size occurred. D1 protein synthesis was rapid. In excess irradiance, quantum yield of O2 evolution and the efficiency of PSII were reduced, associated with a 15- to 20-fold increase in antheraxanthin and zeaxanthin and a sustained increase in nonphotochemical quenching. A decrease in functional PSII center concentration occurred, followed by a decline in the concentration of D1 protein; the latter, however, was not matched by a decrease in DCMU-binding sites. In the most extreme treatments, DCMU-binding site concentration remained 2 times greater than the concentration of D1 protein recognized by antibodies. D1 protein synthesis rates remained unaltered at excess irradiances.

The interaction between the moth, Cactoblastis cactorum, and the cactus, Opuntia stricta, is used as a model to examine the question of whether the CO2 sense of a herbivorous insect can detect the CO2 gradients associated with a plant's metabolic activity. Both the anatomical and the electrophysiological characteristics of CO2-sensitive receptor neurons in C. cactorum indicate an adaptation to the detection of small fluctuations around the atmospheric background. Evidence is provided that further rises in background will impair the function of the sensory organ. In the habitat of the plant, during the diurnal window of the moth's activity, two types of CO2 gradients occur that are detectable by the moth's sensors. The first gradient, associated with soil respiration, is vertical and extends from the soil surface to an altitude of approximately 1 m. Its magnitude is well above the detectability limit of the sensors. The notion that this gradient provides, to a flying insect, a cue for the maintenance of a flight altitude favourable for host detection is supported by field observations of behaviour. The second gradient, associated with CO2 fixation by the plant, extends from the surfaces of photosynthetic organs (cladodes) over a boundary layer distance of approximately 5 mm. Again, its magnitude is well above the detectability limit. The notion that this gradient provides, to a walking insect, a cue to the physiological condition of the plant is supported by the observation that females of C. cactorum, prior to oviposition, actively probe the plant surface with their CO2 sensors. In a simulation of probing, pronounced responses of the sensors to the CO2-fixing capacity of O. stricta are observed. We propose that by probing the boundary layer, females of C. cactorum can detect the healthiest, most active O. stricta cladodes, accounting for earlier observations that the most vigorous plants attract the greatest density of egg sticks.

International audience; The GANIL facility (Caen, France) is dedicated to the acceleration of heavy ion beams including radioactive beams produced by the Isotope Separation On-Line (ISOL) method at the SPIRAL1 facility. To extend the range of radioactive ion beams available at GANIL, using the ISOL method two projects are underway: SPIRAL1 upgrade and the construction of SPIRAL2. For SPIRAL1, a new target ion source system (TISS) using the VADIS FEBIAD ion source coupled to the SPIRAL1 carbon target will be tested on-line by the end of 2013 and installed in the cave of SPIRAL1 for operation in 2015. The SPIRAL2 project is under construction and is being design for using different production methods as fission, fusion or spallation reactions to cover a large area of the chart of nuclei. It will produce among others neutron rich beams obtained by the fission of uranium induced by fast neutrons. The production target made from uranium carbide and heated at 2000 °C will be associated with several types of ion sources. Developments currently in progress at GANIL for each of these projects are presented.

Disturbance or rainforest is often followed by mass mortality of understorey seedlings. Transitions of shade grown plants to full sunlight can cause reductions in the efficiency with which light is used in photosynthesis, called photoinhibition. In order to assess the influence of photoinhibition on mortality and growth after rainforest disturbance this study examined photoinhibition in both simulated and real forest disturbances in northern Papua New Guinea. In an experiment simulating rainforest disturbance, exposure of shade-grown plants to full sunlight resulted in abrupt decreases in the chlorophyll fluorescence parameter F v/F m that is characteristic of photoinhibition. However, in the well-watered plants used in these experiments there were no fatalities during 3 weeks after exposure to full sunlight. Thus, it is unlikely that photoinhibition, alone, is responsible for seedling fatalities after rainforest disturbances, but more likely that fatalities are due to photoinhibition in conjunction with other environmental stress. There were differences between the response of species to the simulated disturbance that concurred with their preferred habitats. For example, species form the genus Barringtonia, which is commonly found in shaded understorey environments, underwent greater reductions in F v/F m and were slower to recover than species that usually inhabit high solar radiation environments. The extent of photoinhibition and the rate of recovery appeared to be dependent on avoidance of direct solar radiation by altering leaf angles and on increasing maximum photosynthetic rates. A field survey of photoinhibition in man-made rainforest gaps corroborated the findings of the simulated disturbance experiment showing that plant species commonly found in shaded environments showed a greater degree of photoinhibition in forest gaps at midday than those species which are classified as species that benefit from gaps or specialist gap inhabitors.

Two direct but independent approaches were developed to identify the average δ18O value of the water fraction in the chloroplasts of transpiring leaves. In the first approach, we used the δ18O value of CO2 in isotopic equilibrium with leaf water to reconstruct the δ18O value of water in the chloroplasts. This method was based on the idea that the enzyme carbonic anhydrase facilitates isotopic equilibrium between CO2 and H2O predominantly in the chloroplasts, at a rate that is several orders of magnitude faster than the non-catalysed exchange in other leaf water fractions. In the second approach, we measured the δ18O value of O2 from photosynthetic water oxidation in the chloroplasts of intact leaves. Since O2 is produced from chloroplast water irreversibly and without discrimination, the δ18O value of the O2 should be identical to that of chloroplast water. In intact, transpiring leaves of sunflower (Helianthus annuus cv. giant mammoth) under the experimental conditions used, the average δ18O value of chloroplasts water was displaced by 3—10 % (depending on relative humidity and atmospheric composition) below the value predicted by the conventional Craig & Gordon model. Furthermore, this δ18O value was always lower than the δ18O value that was measured for bulk leaf water. Our results have implications for a variety of environmental studies since it is the δ18O value of water in the chloroplasts that is the relevant quantity in considering terrestrial plants influence on the δ18O values of atmospheric CO2 and O2, as well as in influencing the δ18O of plant organic matter.

Leaves of avocado (Persea americana) that develop and persist in deep shade canopies have very low rates of photosynthesis but contain high concentrations of lutein epoxide (Lx) that are partially deepoxidized to lutein (L) after 1 h of exposure to 120 to 350 μmol photons m−2 s−1, increasing the total L pool by 5% to 10% (ƊL). Deepoxidation of Lx to L was near stoichiometric and similar in kinetics to deepoxidation of violaxanthin (V) to antheraxanthin (A) and zeaxanthin (Z). Although the V pool was restored by epoxidation of A and Z overnight, the Lx pool was not. Depending on leaf age and pretreatment, the pool of ƊL persisted for up to 72 h in the dark. Metabolism of ƊL did not involve epoxidation to Lx. These contrasting kinetics enabled us to differentiate three states of the capacity for nonphotochemical chlorophyll fluorescence quenching (NPQ) in attached and detached leaves: ƊpH dependent (NPQƊpH) before deepoxidation; after deepoxidation in the presence of ƊL, A, and Z (NPQƊLAZ); and after epoxidation of A+Z but with residual ƊL (NPQƊL). The capacity of both NPQƊLAZ and NPQƊL was similar and 45% larger than NPQƊpH, but dark relaxation of NPQƊLAZ was slower. The enhanced capacity for NPQ was lost after metabolism of ƊL. The near equivalence of NPQƊLAZ and NPQƊL provides compelling evidence that the small dynamic pool ƊL replaces A+Z in avocado to “lock in” enhanced NPQ. The results are discussed in relation to data obtained with other Lx-rich species and in mutants of Arabidopsis (Arabidopsis thaliana) with increased L pools.

The contributions of Hubert Ziegler to our understanding of physiological plant ecology through natural abundance studies of the stable isotopes of carbon, hydrogen and nitrogen are reviewed. These have served to expand our knowledge of plant distributions and physiological functions, especially the processes linking pathways of photosynthetic carbon metabolism and transpiration. They have also served to indicate food chain relationships and organism to organism interactions. His leadership has placed stable isotope techniques firmly on centre-stage, as indicators and integrators of plant biospheric processes in the environmental context.

Synthesis of the photosystem II D1 protein as a precursor with a carboxyl-terminal extension occurs in almost all eukaryotic photosynthetic organisms examined so far, as well as in cyanobacteria. Processing of the D1 precursor has been recently postulated to play a regulatory role in the light-dependent migration of photosystem II units from the unstacked to the stacked thylakoids (Bowyer, J. M., Packer, J. C. L., McCormack, B. A., Whitelegge, J. P., Robinson, C., and Taylor, M. A. (1992) J. Biol. Chem. 267, 5424-5433). To test this hypothesis, site-directed mutagenesis and chloroplast transformation have been used to create a "preprocessed" mutant Chlamydomonas strain which synthesizes mature D1 protein directly. We have found that this strain is indistinguishable from wild type in terms of photosynthetic performance and cell doubling time under CO2- and light-saturated photoautotrophic growth conditions.

We have investigated the diurnal response of photosynthesis and variable photosystem II (PSII) chlorophyll fluorescence at 77 K for thalli of the chlorophyte macroalga, Ulva rotundata, grown in outdoor culture and transplanted to an intertidal sand flat in different seasons. The physiological response in summer indicated synergistic effects of high PFD and aerial exposure, the latter probably attributable to temperature, which usually increased by 8 to 10° C during midday emersion. Except at extreme emersed temperatures in summer (38° C), the light-saturated photosynthesis rate (Pm) did not decline at midday. In contrast, light-limited quantum yield of photosynthetic O2 exchange (τ) and the ratio of variable to maximum fluorescence yield (Fv/Fm) reversibly declined during midday low tides in all seasons. Shade-grown thalli exhibited a fluorescence response suggestive of greater photodamage to PSII, whereas sun-grown thalli had greater photoprotective capacity. The fluorescence decline was smaller when high tide occurred at midday, and was delayed during morning cloudiness. These results suggest that the diurnal response to PFD in this shallow water species is modified by tidal and meteorological factors. U. rotundata has a great capacity for photoprotection which allows it to tolerate and even thrive in the harsh intertidal environment.

Clonal tissue of the marine chlorophyte macroalga, Ulva rotundata Blid., was transferred from 100 to 1700 μmol photons · m−2 · s−1 under limiting (1.5 μM NH 4 + maximum, N/P=2) and sufficient (15 μM NH 4 + maximum, N/P=20) nitrogen supply at 18° C and 11 h light-13 h darkness daily. Photoinhibition was assayed by light-response curves (photosynthetic O2 exchange), and chlorophyll fluorescence at 77 K and room temperature. Daily surface-area growth rate (μSA) in N-sufficient plants increased sixfold over 3 d and was sustained at that level. During this period, respiration (R d) doubled and light-saturated net photosynthesis capacity (P m) increased by nearly 50%, indicating acclimation to high light. Quantum yield (ϕ) decreased by 25% on the first day, but recovered completely within one week. The ratio of variable to maximum fluorescence (F v/F m) also decreased markedly on the first day, because of an increase in initial fluorescence (F o) and a decrease in F m, and partially recovered over several days. Under the added stress of N deficiency, μSA accelerated fivefold over 4 d, despite chronic photoinhibition, then declined along with tissue-N. Respiration doubled, but P m decreased by 50% over one week, indicating inability to acclimate to high light. Both ϕ and F v/F m decreased markedly on the first day and did not significantly recover. Changes in F o, F m and xanthophyll-cycle components indicate concurrent photodamage to photosystem II (PSII) and photoprotection by thermal deexcitation in the antenna pigments. Increasing μSA coincided with photoinhibition of PSII. Insufficient diel-carbon balance because of elevated R d and declining P m and tissue-N, rather than photochemical damage per se, was the apparent proximate cause of decelerating growth rate and subsequent tissue degeneration under N deficiency in U. rotundata.

Thalli of the chlorophyte macroalga Ulva rotundata were acclimated to sun (100 % I,) and shade (9% I,) in outdoor tumble tanks under nutrient-sufficient conditions. Diurnal patterns were determined for area1 photosynthetic O2 exchange capacity (P,,) and quantum yleld (@), respiration (Rd) , and photosystem (PS) I1 chlorophyll fluorescence using 2 independent techniques. Net P, was maximal at midday in sun-grown thalli and at midmorning in shade-grown thalli exposed to 100 "L I,. A slight afternoon decline in net P, in shade-grown thalli exposed to 100 % I, could be explained entirely by a concurrent rise in Rd. Sun thalli exhibited a larger diurnal fluctuation of net P, which was inconsistent with photoinhibition or end product inhibition by accumulated photosynthate. This leaves endogenous control as the most likely cause, with slight modification by Rd Fluorescence data were consistent with simultaneous damage to PSI1 and photoprotection by thermal deexcitation, with sun thalli showing greater capacity for protection. Both of these processes reduced @ but apparently did not affect P,,. In that U. rotundata thalli were light-saturated in our tumble tanks for most of the day, we conclude that photoinhibition (E damage + protection) had little effect on ~ntegrated diurnal production under these conditions. Our results demonstrate the utility of fluorescence analysis and the importance of precise determination of P,, @ and Rd in ascribing mechanisms to diurnal fluctuations in photosynthetic performance. The validity of using photosynthetic light-response (P-I) curves determined at one time of day to predict integrated diurnal production will depend on the extent to which P,, @ and Rd vary. This in turn depends on species, irradiance history, and possibly other factors such as temperature and nutrition.

Leaves of avocado (Persea americana) accumulate the α-xanthophyll lutein-epoxide (Lx) in excess of violaxanthin (V) in shaded habitats. On transfer to strong light both Lx and V are deepoxidised by violaxanthin de-epoxidase (VDE) to lutein (L) and to antheraxanthin (A) and zeaxanthin (Z) respectively, with about the same kinetics. However, whereas Z and A are rapidly epoxidised to form V by zeaxanthin epoxidase (ZE) when in shade or darkness, lutein is converted to Lx about 250 × more slowly, and hence Lx remains low in sun leaves. We investigated relationships between xanthophyll pool sizes and PSII efficiency (dark adapted Fv/Fm) in avocado leaves and discuss implications for the regulation of light harvesting efficiency arising from the differing epoxidation kinetics of these two co-located xanthophyll cycles

Attention is drawn to literature evidence that leaf virus infection may interfere with synthesis of ribulose-1,5-bisphosphate carboxylase oxygenase, and with the activity of photosystem II, two main requirements for shade-sun acclimation. Preliminary experiments which show that virus infections resulting in these interactions can lead to photosynthetic physiology diagnostic of nitrogen limited plants and/or shade ecotypes are presented. Other observations on the diversity of virus infections in sun populations of wild species commonly found in the shade are presented. Together, this evidence leads to the hypothesis that virus infection may well confine individuals of some species to shaded habitats because infection prevents acclimation to bright light, and predisposes them to photoinhibition.

Water stress experiments were performed with grapevines (Vitis vinifera L.) and other C3 plants in the field, in potted plants in the laboratory, and with detached leaves. It was found that, in all cases, the ratio of steady state chlorophyll fluorescence (Fs) normalized to dark-adapted intrinsic fluorescence (Fo) inversely correlated with non-photochemical quenching (NPQ). Also, at high irradiance, the ratio Fs/Fo was positively correlated with CO2 assimilation in air, with electron transport rate calculated from fluorescence, and with stomatal conductance, but no clear correlation was observed with qP. The significance of these relationships is discussed. The ratio Fs/Fo, measured with a portable instrument (PAM-2000) or with a remote sensing FIPAM system, provides a good method for the early detection of water stress, and may become a useful guide to irrigation requirements.

We found similarities between the effects of low night temperatures (5°C–10°C) and slowly imposed water stress on photosynthesis in grapevine (Vitis vinifera L.) leaves. Exposure of plants growing outdoors to successive chilling nights caused light- and CO2-saturated photosynthetic O2 evolution to decline to zero within 5 d. Plants recovered after four warm nights. These photosynthetic responses were confirmed in potted plants, even when roots were heated. The inhibitory effects of chilling were greater after a period of illumination, probably because transpiration induced higher water deficit. Stomatal closure only accounted for part of the inhibition of photosynthesis. Fluorescence measurements showed no evidence of photoinhibition, but nonphotochemical quenching increased in stressed plants. The most characteristic response to both stresses was an increase in the ratio of electron transport to net O2evolution, even at high external CO2 concentrations. Oxygen isotope exchange revealed that this imbalance was due to increased O2 uptake, which probably has two components: photorespiration and the Mehler reaction. Chilling- and drought-induced water stress enhanced both O2 uptake processes, and both processes maintained relatively high rates of electron flow as CO2 exchange approached zero in stressed leaves. Presumably, high electron transport associated with O2uptake processes also maintained a high ΔpH, thus affording photoprotection.

Transfer of Chlamydomonas reinhardtii cells grown photoautotrophically in low light to higher light intensities has a dramatic transient effect on the differential expression of the two major chloroplast encoded photosynthetic proteins. Synthesis of the D1 protein of Photosystem II increases more than 10-fold during the first six hours in high light (HL), whereas synthesis of the large subunit (LSU) of Rubisco drops dramatically within 15 min and only gradually resumes at about 6 h. Synthesis of the chloroplast-encoded ATP synthase beta subunit, the nuclear-encoded Rubisco small subunit and the nuclear-encoded beta-tubulin is not noticeably affected. Up regulation of psbA mRNA translation accounts for a substantial fraction of the increased D1 synthesis, since accumulation of psbA mRNA increases 4.2- and 6.3-fold less than D1 synthesis at 6 and 18 h in HL. Down-regulation of LSU synthesis is not correlated with a reduction in the steady-state level of the rbcL transcript. Primer extension mapping of the 5' ends of the rbcL mRNAs reveals transcripts with start points located at -93 and -168 relative to the first translated ATG. Transfer of low light (LL)-grown cells to HL temporarily decreases the ratio of the -93 to -168 transcripts, but this ratio normalizes after 6 h in HL, coincident with the recovery in the synthesis of LSU. These several distinct effects of temporary light stress were correlated with a rapid, sustained increase in the reduction state of QA, a transient decline in photosynthetic efficiency, a less rapid drop in total chlorophyll content and a delay in cell division.